##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364173_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2128678 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.130122075767215 33.0 31.0 34.0 28.0 34.0 2 31.078775653245817 33.0 31.0 34.0 27.0 34.0 3 31.203987639276583 34.0 31.0 34.0 27.0 34.0 4 34.53713713393947 37.0 35.0 37.0 32.0 37.0 5 34.65097398479244 37.0 35.0 37.0 32.0 37.0 6 34.69715710877831 37.0 35.0 37.0 32.0 37.0 7 34.59436185275556 37.0 35.0 37.0 31.0 37.0 8 34.527152533168476 37.0 35.0 37.0 31.0 37.0 9 36.004194622202135 39.0 37.0 39.0 31.0 39.0 10-11 35.99643417181932 39.0 37.0 39.0 31.0 39.0 12-13 35.9793874414073 39.0 37.0 39.0 31.0 39.0 14-15 37.31552071285559 40.0 38.0 41.0 31.0 41.0 16-17 37.35948579353007 40.0 38.0 41.0 31.0 41.0 18-19 37.2690801520944 40.0 38.0 41.0 31.0 41.0 20-21 37.26412261506907 40.0 38.0 41.0 30.5 41.0 22-23 37.32383972587681 40.0 38.0 41.0 30.5 41.0 24-25 37.19172063600037 40.0 38.0 41.0 30.0 41.0 26-27 37.23563521584758 40.0 37.5 41.0 30.0 41.0 28-29 37.334152229693736 40.0 38.0 41.0 30.0 41.0 30-31 37.23664781615632 40.0 37.5 41.0 30.0 41.0 32-33 37.05904509747364 40.0 37.0 41.0 30.0 41.0 34-35 37.01464641434731 40.0 37.0 41.0 30.0 41.0 36-37 37.034358648889125 40.0 37.0 41.0 30.0 41.0 38-39 36.86998855627765 40.0 37.0 41.0 30.0 41.0 40-41 36.87523101192383 40.0 37.0 41.0 30.0 41.0 42-43 36.80974365310301 40.0 37.0 41.0 30.0 41.0 44-45 36.80537192567405 40.0 37.0 41.0 30.0 41.0 46-47 36.740655467853756 40.0 36.5 41.0 30.0 41.0 48-49 36.662215938718774 40.0 36.0 41.0 30.0 41.0 50-51 36.56381754309483 40.0 36.0 41.0 30.0 41.0 52-53 36.431023621233464 40.0 36.0 41.0 30.0 41.0 54-55 36.259213229995325 40.0 35.0 41.0 29.0 41.0 56-57 36.08066015620963 39.0 35.0 41.0 29.0 41.0 58-59 35.86945348239612 39.0 35.0 41.0 29.0 41.0 60-61 35.63212190852727 39.0 35.0 41.0 29.0 41.0 62-63 35.34965974186795 38.0 35.0 40.0 28.0 41.0 64-65 35.04577723826713 37.5 34.0 40.0 28.0 41.0 66-67 34.732623722329066 37.0 34.0 40.0 28.0 41.0 68-69 34.49228699690606 37.0 34.0 39.5 28.0 41.0 70-71 34.30692758604167 36.0 34.0 39.0 28.0 41.0 72-73 33.99172843426766 36.0 34.0 39.0 28.0 41.0 74-75 33.648319755265945 35.5 34.0 38.5 27.0 40.0 76-77 33.30816238999041 35.0 34.0 37.0 27.0 39.5 78-79 32.963109028232545 35.0 34.0 37.0 26.5 39.0 80-81 32.65649266821943 35.0 33.0 36.5 26.0 39.0 82-83 32.382268008595005 35.0 33.0 36.0 26.0 37.5 84-85 32.12860376252303 35.0 33.0 36.0 26.0 37.0 86-87 31.915363197252006 35.0 33.0 35.0 26.0 37.0 88-89 31.73825045403767 35.0 33.0 35.0 25.0 36.0 90-91 31.58443667853945 35.0 33.0 35.0 25.0 36.0 92-93 31.449626246900657 35.0 33.0 35.0 25.0 36.0 94-95 31.31747544720244 35.0 33.0 35.0 24.5 36.0 96-97 31.22147196522912 35.0 33.0 35.0 24.0 35.0 98-99 31.141104478930117 35.0 33.0 35.0 24.0 35.0 100-101 31.04214000426556 35.0 32.5 35.0 23.0 35.0 102-103 30.077670742122578 34.0 30.5 35.0 20.0 35.0 104-105 30.74340717572127 35.0 32.0 35.0 20.0 35.0 106-107 30.835881002199486 35.0 32.0 35.0 20.0 35.0 108-109 30.795038047088383 35.0 32.0 35.0 20.0 35.0 110-111 30.724077807916462 35.0 32.0 35.0 19.0 35.0 112-113 30.856419336320478 35.0 33.0 35.0 20.0 35.0 114-115 30.834529224241525 35.0 33.0 35.0 19.5 35.0 116-117 30.737829065739394 35.0 33.0 35.0 18.5 35.0 118-119 30.499106487688604 35.0 33.0 35.0 10.0 35.0 120-121 30.42944447210898 35.0 33.0 35.0 2.0 35.0 122-123 30.288021250748116 35.0 32.5 35.0 2.0 35.0 124-125 29.423346790825104 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5260529756301615 1101 2 0.5089919202618667 1101 3 0.4366398692793183 1101 4 0.4595194422580491 1101 5 0.3437548451201238 1101 6 0.4213662028966638 1101 7 0.3476491307554923 1101 8 0.40127121658133547 1101 9 0.5158648657911797 1101 10-11 0.4951919472202846 1101 12-13 0.5347897515746425 1101 14-15 0.5511165602432015 1101 16-17 0.6177325247100782 1101 18-19 0.740974711722302 1101 20-21 0.7854531766772297 1101 22-23 0.8153773339277066 1101 24-25 0.9314686345760848 1101 26-27 0.9261833247260185 1101 28-29 0.8616055215693166 1101 30-31 0.9527324706056106 1101 32-33 1.1378013943409684 1101 34-35 0.9439793350982839 1101 36-37 0.8815610525537068 1101 38-39 0.9538232236786115 1101 40-41 0.8963086031546652 1101 42-43 0.8753056647171391 1101 44-45 0.8037237948243785 1101 46-47 0.68000985417072 1101 48-49 0.6529834425387904 1101 50-51 0.6512474296709456 1101 52-53 0.6126956160172838 1101 54-55 0.6187536615496967 1101 56-57 0.6009455030857396 1101 58-59 0.626231952735786 1101 60-61 0.4732979258669445 1101 62-63 0.4629638565539054 1101 64-65 0.4709610838131937 1101 66-67 0.5398028014980625 1101 68-69 0.6104642682433266 1101 70-71 0.5027483414952059 1101 72-73 0.42494835158655064 1101 74-75 0.5148318963111791 1101 76-77 0.5309639358027383 1101 78-79 0.5308328335757793 1101 80-81 0.6143672704052605 1101 82-83 0.5812551839939388 1101 84-85 0.48692911901252245 1101 86-87 0.5544567356528738 1101 88-89 0.6076722512837094 1101 90-91 0.5938175425835688 1101 92-93 0.5821401334368446 1101 94-95 0.6595484703356149 1101 96-97 0.6942653524173394 1101 98-99 0.6324428749402742 1101 100-101 0.5688731801278877 1101 102-103 0.46813585514096445 1101 104-105 0.548787763172033 1101 106-107 0.6074286841955008 1101 108-109 0.5663520876912109 1101 110-111 0.5296037956570316 1101 112-113 0.6411301208612379 1101 114-115 0.5979377009434899 1101 116-117 0.5613538764737775 1101 118-119 0.5142082099298229 1101 120-121 0.4990393291024482 1101 122-123 0.44260636260105457 1101 124-125 0.4244961676778445 1102 1 0.21520557721175848 1102 2 0.24412030832981912 1102 3 0.26971898889835444 1102 4 0.19800485482053176 1102 5 0.17457800305201943 1102 6 0.25021540805803966 1102 7 0.24618447161513046 1102 8 0.32462082025026007 1102 9 0.30658749609668234 1102 10-11 0.27206286312098626 1102 12-13 0.3890083012483956 1102 14-15 0.4431733712245318 1102 16-17 0.42562836868589926 1102 18-19 0.4790934688348898 1102 20-21 0.5378160155772207 1102 22-23 0.5559389987627341 1102 24-25 0.6679686912081308 1102 26-27 0.7342573430756758 1102 28-29 0.5616170563041649 1102 30-31 0.5829855582578602 1102 32-33 0.7210737920384531 1102 34-35 0.7181626139212156 1102 36-37 0.5124254153654135 1102 38-39 0.563905875755367 1102 40-41 0.5445667869229496 1102 42-43 0.5205522081617033 1102 44-45 0.32585305205433457 1102 46-47 0.33986439370838184 1102 48-49 0.35008700702405093 1102 50-51 0.3173910860218143 1102 52-53 0.26770337400363786 1102 54-55 0.32088486828142493 1102 56-57 0.32812808160116447 1102 58-59 0.2809694339027544 1102 60-61 0.2638677647940213 1102 62-63 0.32485366277583694 1102 64-65 0.35976256675027685 1102 66-67 0.3017092629122331 1102 68-69 0.29776785840762443 1102 70-71 0.3177228614632881 1102 72-73 0.28732471090626177 1102 74-75 0.30860032159729656 1102 76-77 0.2173169519769118 1102 78-79 0.2578321391891336 1102 80-81 0.32385198024801554 1102 82-83 0.27200050000893583 1102 84-85 0.17143290447785375 1102 86-87 0.18594931389527503 1102 88-89 0.13532211813537032 1102 90-91 0.11016051849472674 1102 92-93 0.23748170796542922 1102 94-95 0.2504157563035143 1102 96-97 0.2733813531449485 1102 98-99 0.23651924146371073 1102 100-101 0.22770077739766492 1102 102-103 0.17563652480249914 1102 104-105 0.2784072188926281 1102 106-107 0.18353770454454832 1102 108-109 0.15372518230816468 1102 110-111 0.10010750502820542 1102 112-113 0.20543582905283841 1102 114-115 0.13859555801123946 1102 116-117 0.1999682763183479 1102 118-119 0.006898400657540549 1102 120-121 -0.01685464738664777 1102 122-123 0.08945039006994904 1102 124-125 0.0336961164952001 1103 1 0.34514211686503415 1103 2 0.028461280847430714 1103 3 0.2739115897034843 1103 4 0.3095741758380015 1103 5 0.17635271130074415 1103 6 0.15740003863662366 1103 7 0.2862642463794316 1103 8 0.27540142244347265 1103 9 0.45210643080860535 1103 10-11 0.30776581411854664 1103 12-13 0.3634289024504227 1103 14-15 0.4574191894715085 1103 16-17 0.37506417031115546 1103 18-19 0.4490089123891323 1103 20-21 0.5615417688868263 1103 22-23 0.5302271542055834 1103 24-25 0.7735519833054596 1103 26-27 0.7115211998074571 1103 28-29 0.574960100883743 1103 30-31 0.6666160739771954 1103 32-33 0.8152059904357145 1103 34-35 0.6962823364473536 1103 36-37 0.604510385379541 1103 38-39 0.7496343799114555 1103 40-41 0.7064523492423049 1103 42-43 0.6614753615956843 1103 44-45 0.6836389852975628 1103 46-47 0.619151813882489 1103 48-49 0.49680460831881135 1103 50-51 0.5094760127530122 1103 52-53 0.459219671270354 1103 54-55 0.4457518950115542 1103 56-57 0.5089722155579324 1103 58-59 0.42409969203456654 1103 60-61 0.32063516417934323 1103 62-63 0.34563060431542425 1103 64-65 0.4221565844436981 1103 66-67 0.43828724322045076 1103 68-69 0.4768241369263393 1103 70-71 0.4846427152232664 1103 72-73 0.42300300145567604 1103 74-75 0.4975010856383548 1103 76-77 0.4606940959291137 1103 78-79 0.4608903160970641 1103 80-81 0.4304310502039428 1103 82-83 0.36802524442595796 1103 84-85 0.34643952406063505 1103 86-87 0.3405141641109104 1103 88-89 0.301569950336976 1103 90-91 0.30863346433836725 1103 92-93 0.25989755756764055 1103 94-95 0.3331933278261161 1103 96-97 0.342988092037114 1103 98-99 0.34552642956774804 1103 100-101 0.3819155769344107 1103 102-103 0.2461760648932554 1103 104-105 0.30635451846535133 1103 106-107 0.35106232901375023 1103 108-109 0.3788556505785863 1103 110-111 0.4073113856889492 1103 112-113 0.37350771204090805 1103 114-115 0.4358928962518469 1103 116-117 0.4446630066976276 1103 118-119 0.2839651902188649 1103 120-121 0.28424482554632036 1103 122-123 0.2977506705757875 1103 124-125 0.1542794318571019 1104 1 0.24123934656915935 1104 2 0.27432631431631904 1104 3 0.3353969658652076 1104 4 0.2825969498044927 1104 5 0.28002978541150725 1104 6 0.24050647207186415 1104 7 0.22318476478604055 1104 8 0.22297310142766236 1104 9 0.36326157893327604 1104 10-11 0.31711940749919876 1104 12-13 0.351371203247254 1104 14-15 0.36363291876693893 1104 16-17 0.26985145935691435 1104 18-19 0.5221187041836117 1104 20-21 0.5039460142267558 1104 22-23 0.5752064390131366 1104 24-25 0.7432356220420147 1104 26-27 0.7058879823421123 1104 28-29 0.6235220603812053 1104 30-31 0.6389440754284479 1104 32-33 0.7568397886571603 1104 34-35 0.7565074782164203 1104 36-37 0.6560229109336575 1104 38-39 0.7346710945736987 1104 40-41 0.6280454414308565 1104 42-43 0.5625869232730807 1104 44-45 0.5846179969330763 1104 46-47 0.5280875984852713 1104 48-49 0.4044370372813333 1104 50-51 0.42367195230339405 1104 52-53 0.43319509555794156 1104 54-55 0.43674582065097667 1104 56-57 0.3928399514332739 1104 58-59 0.3893286191269425 1104 60-61 0.4485931649338539 1104 62-63 0.37937271503889036 1104 64-65 0.37541713491721396 1104 66-67 0.4692988018390096 1104 68-69 0.40198360057448923 1104 70-71 0.32834646078156027 1104 72-73 0.3202197373429243 1104 74-75 0.36781935018444756 1104 76-77 0.3095871012803997 1104 78-79 0.39386210715401404 1104 80-81 0.32864106172559815 1104 82-83 0.3395906051914821 1104 84-85 0.39041544328667754 1104 86-87 0.3891064996596434 1104 88-89 0.42852177094578536 1104 90-91 0.39843967575228945 1104 92-93 0.38785263754799715 1104 94-95 0.46861996052825106 1104 96-97 0.39791654262908693 1104 98-99 0.3656694689062192 1104 100-101 0.4471425710053438 1104 102-103 0.42412130983043994 1104 104-105 0.4341452916229507 1104 106-107 0.45220507393819176 1104 108-109 0.48411881126832057 1104 110-111 0.48914722925950826 1104 112-113 0.5216638220030596 1104 114-115 0.48826805917794047 1104 116-117 0.4825897371129315 1104 118-119 0.4504301464518612 1104 120-121 0.41295394320487233 1104 122-123 0.36357838823399646 1104 124-125 0.30374750046704335 1105 1 0.3701342093606286 1105 2 0.3497111273365512 1105 3 0.33110333203789466 1105 4 0.3269535882202419 1105 5 0.28373380630818446 1105 6 0.2638956182530521 1105 7 0.3253568214152338 1105 8 0.23095613935064563 1105 9 0.28423162396126145 1105 10-11 0.17287525669004822 1105 12-13 0.24807921144254408 1105 14-15 0.32173313814247706 1105 16-17 0.33251934669802097 1105 18-19 0.44854853612745416 1105 20-21 0.5840489659147323 1105 22-23 0.6037956665543192 1105 24-25 0.6994053943328424 1105 26-27 0.6470816067817395 1105 28-29 0.5464750494552533 1105 30-31 0.499772057502895 1105 32-33 0.6486104061696523 1105 34-35 0.6288430000897449 1105 36-37 0.5851317348699752 1105 38-39 0.5893776221488025 1105 40-41 0.5361653626071856 1105 42-43 0.5261782218708575 1105 44-45 0.4739559464305856 1105 46-47 0.4183228342810352 1105 48-49 0.34328517983590956 1105 50-51 0.2094621159786243 1105 52-53 0.27050793874395396 1105 54-55 0.30894289465563674 1105 56-57 0.2409412289828623 1105 58-59 0.2746205969324578 1105 60-61 0.20885323282168855 1105 62-63 0.218362578802072 1105 64-65 0.2978501982034345 1105 66-67 0.3156779731857924 1105 68-69 0.29293629484207173 1105 70-71 0.3528281613701907 1105 72-73 0.3282771974287684 1105 74-75 0.36843325582661635 1105 76-77 0.3203939610480404 1105 78-79 0.28814053539087325 1105 80-81 0.20880027780391686 1105 82-83 0.2344066640737239 1105 84-85 0.28239296058802665 1105 86-87 0.2980113018757997 1105 88-89 0.25797890707391247 1105 90-91 0.24769073969070732 1105 92-93 0.31677607515607775 1105 94-95 0.389485011633532 1105 96-97 0.281832276565428 1105 98-99 0.3538770044229871 1105 100-101 0.33337475909516456 1105 102-103 0.31910144745070923 1105 104-105 0.3139338379800307 1105 106-107 0.21650720197972007 1105 108-109 0.28309796153397926 1105 110-111 0.31953839955882657 1105 112-113 0.2980646468023771 1105 114-115 0.3382291702524505 1105 116-117 0.2546326965086969 1105 118-119 0.09627668980488124 1105 120-121 0.22584355300628545 1105 122-123 0.12586998559095264 1105 124-125 0.07782147465583122 1106 1 0.2786369797769126 1106 2 0.2434624400564509 1106 3 0.3720166196346959 1106 4 0.29507101033755134 1106 5 0.2807318694062886 1106 6 0.18611071436121307 1106 7 0.22297671716870582 1106 8 0.31087332203587437 1106 9 0.36396407206790826 1106 10-11 0.3302394998666145 1106 12-13 0.356889886209693 1106 14-15 0.3549757239607203 1106 16-17 0.40769507546589523 1106 18-19 0.39098273146178286 1106 20-21 0.48429379947088336 1106 22-23 0.5557129705218173 1106 24-25 0.6401499710677783 1106 26-27 0.6437113051141878 1106 28-29 0.6204048238099915 1106 30-31 0.6679258040607081 1106 32-33 0.7510226381689762 1106 34-35 0.8039657582251962 1106 36-37 0.5911534797362634 1106 38-39 0.594511235448735 1106 40-41 0.5438302412305447 1106 42-43 0.5006723001236182 1106 44-45 0.40258073002005546 1106 46-47 0.4073328336424922 1106 48-49 0.31860082496721276 1106 50-51 0.3087846621512256 1106 52-53 0.3649077099943483 1106 54-55 0.3908143483039197 1106 56-57 0.35030219191586554 1106 58-59 0.31840821401301866 1106 60-61 0.33244909942565215 1106 62-63 0.3363403054342555 1106 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74-75 -2.4164903649751395 2216 76-77 -2.3204139480894455 2216 78-79 -2.2684535178961127 2216 80-81 -2.2898914446775613 2216 82-83 -2.287638136045942 2216 84-85 -2.368858780446992 2216 86-87 -2.2111718172848036 2216 88-89 -2.1451897766675287 2216 90-91 -2.3141143634527026 2216 92-93 -2.2869018633797253 2216 94-95 -2.183177737007263 2216 96-97 -2.252831952160154 2216 98-99 -2.2023400569657596 2216 100-101 -2.221921103687329 2216 102-103 -2.2439122111863696 2216 104-105 -2.172443280538939 2216 106-107 -2.214721094538472 2216 108-109 -2.187767133592306 2216 110-111 -2.2355175071013598 2216 112-113 -2.305188817061836 2216 114-115 -2.364079755459233 2216 116-117 -2.271397868291924 2216 118-119 -2.0891877425635776 2216 120-121 -1.9771417767029398 2216 122-123 -1.99917356078668 2216 124-125 -1.9073591435813597 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32279.0 3 6889.0 4 5027.0 5 4202.0 6 4718.0 7 4974.0 8 4834.0 9 5051.0 10 5890.0 11 6932.0 12 7854.0 13 7918.0 14 7804.0 15 7066.0 16 6473.0 17 5771.0 18 5440.0 19 4916.0 20 5016.0 21 5153.0 22 5947.0 23 8498.0 24 13800.0 25 20669.0 26 24484.0 27 21402.0 28 23962.0 29 29542.0 30 35239.0 31 42740.0 32 55688.0 33 75927.0 34 108036.0 35 170816.0 36 340831.0 37 685720.0 38 319766.0 39 1404.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.762327833403845 18.7753020260424 12.350632256851409 24.111737883702343 2 15.63223533990293 20.990910791519745 39.52879753409746 23.848056334479864 3 18.835689030159156 25.78542157910098 29.27170874929912 26.107180641440742 4 12.277186679563355 16.965208081298016 37.113228599440326 33.6443766396983 5 13.171246174865573 39.424252351009514 33.94369420920119 13.460807264923725 6 31.61178359718611 37.13592027759273 17.645892625433035 13.606403499788122 7 28.0190579183518 33.54546902313136 20.99584294223292 17.43963011628393 8 25.462798976641842 36.69676672563911 19.860401620160495 17.98003267755856 9 26.293947227858265 17.160881887721906 19.446497117201847 37.09867376721798 10-11 24.347945986106833 27.955128263852234 27.64779017950996 20.049135570530964 12-13 24.708098361949574 25.643466119960838 27.50081273007778 22.14762278801181 14-15 22.117352403932887 26.86959246647423 25.079985454627458 25.93306967496542 16-17 22.44602183907803 29.80810766434802 25.72348082591713 22.02238967065682 18-19 22.341250676508615 28.64936464565982 27.311427921765535 21.697956756066027 20-21 23.261790517033603 27.93853234414032 26.949112249227024 21.85056488959905 22-23 23.02176966061168 27.9461727956533 26.63544968846452 22.396607855270496 24-25 22.736442189657367 27.8932419183062 26.994391167556998 22.375924724479436 26-27 22.432371288199835 28.25108694349867 26.83610463694431 22.480437131357185 28-29 22.45446774364423 28.23146709788992 26.957388437074897 22.356676721390958 30-31 22.564012457417814 27.919583975990953 26.99704631973883 22.519357246852405 32-33 22.279377117861753 28.08522512932081 26.939131014868313 22.696266737949124 34-35 22.783682757295356 28.064146378651202 26.78830900979825 22.36386185425519 36-37 22.801153482471648 27.73284312285998 26.644364439380503 22.82163895528787 38-39 22.477466989059675 27.971595518796715 26.965836475694 22.58510101644961 40-41 22.701375822451485 27.773737459156372 27.06087961494824 22.464007103443905 42-43 22.51771254644677 27.80931451041777 26.91661109536036 22.7563618477751 44-45 22.595660580869197 27.95945077494325 26.802179950692263 22.642708693495287 46-47 22.619700285958707 28.10052855617395 26.575110082593067 22.704661075274274 48-49 22.579710887058223 28.06092883422527 26.64489037352701 22.714469905189503 50-51 22.45440223466574 28.046563052802952 26.686751473534397 22.81228323899691 52-53 22.772693288184037 27.848371837155295 26.787859768777412 22.59107510588326 54-55 22.444905266985536 27.77843810912631 26.946115239356544 22.830541384531617 56-57 22.451572531800508 28.269351784711372 26.695422366071185 22.58365331741693 58-59 22.57643503169123 27.86197085562791 26.651474484227677 22.91011962845318 60-61 22.555662707777095 27.911052431892102 26.921733328791436 22.611551531539362 62-63 22.420073871750315 27.88749017169321 26.90732579142683 22.785110165129648 64-65 22.265343403363623 27.889885801947877 26.843741727353294 23.001029067335203 66-67 22.245521345750277 28.29600906136765 26.59437178573492 22.86409780714715 68-69 22.46718998565164 27.87653173694106 26.86225666747789 22.794021609929406 70-71 22.123042578110137 27.989917453910472 26.986666065340646 22.90037390263874 72-73 22.399695764116657 27.893652740295728 26.809542276737673 22.89710921884994 74-75 22.23105671879907 28.129512101681325 26.7041019650713 22.93532921444831 76-77 22.414564590698557 27.94600101142991 26.84991728401239 22.78951711385915 78-79 22.528219026720166 28.041376161141248 26.72913915467179 22.701265657466795 80-81 22.359264519141735 28.186887191284875 26.751427332531247 22.70242095704214 82-83 22.496405576412595 28.122168826217813 26.653675613753258 22.727749983616334 84-85 22.51642872210923 27.87103870116898 26.652139574720792 22.960393002001 86-87 22.46694389888283 27.752889832258298 26.885018161505503 22.89514810735337 88-89 22.47284176345791 27.758574035203765 26.832840372896072 22.935743828442252 90-91 22.4407661253479 28.00985784715686 26.840248522603076 22.709127504892162 92-93 22.5130612116426 27.741022281456956 26.888455537855084 22.857460969045363 94-95 22.381308962651936 27.60347032289524 26.899394835667962 23.11582587878486 96-97 22.525545641561916 27.587907026126057 26.84893789041929 23.037609441892737 98-99 22.21679234355782 27.670562474082445 26.750370334693287 23.362274847666452 100-101 22.131022762564466 27.761993607293494 27.00310050494206 23.103883125199978 102-103 22.40105612173588 27.741564944087326 26.80566631861943 23.05171261555736 104-105 22.253709327426506 27.81849332204778 26.851468801807194 23.07632854871852 106-107 22.03356258209134 28.029079425648444 26.87572398982126 23.06163400243895 108-109 22.245849651409948 28.108119408271875 26.45671791910963 23.189313021208548 110-111 22.082403943182392 28.23796553285766 26.657375897053328 23.022254626906623 112-113 22.22554251557327 28.202117532746396 26.740499340796475 22.83184061088386 114-115 22.044236359586424 28.394255015794336 26.57717795954657 22.984330665072665 116-117 22.133255874563794 28.240889252995238 26.6351708405334 22.99068403190756 118-119 22.146106881025908 28.444614695551728 26.292048749440134 23.117229673982227 120-121 21.995912742460444 28.427746317235155 26.50784057343894 23.068500366865464 122-123 22.267230351590445 28.34548920889507 26.43369047213507 22.95358996737942 124-125 22.242246256823798 28.42980647718348 26.324188125281438 23.00375914071128 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2862.0 1 2347.0 2 1722.5 3 1398.0 4 999.5 5 751.0 6 657.5 7 617.0 8 600.0 9 608.5 10 556.5 11 492.5 12 485.0 13 509.5 14 572.5 15 652.5 16 745.0 17 870.0 18 1112.5 19 1398.5 20 1846.0 21 2555.5 22 3430.0 23 4567.5 24 6017.0 25 8004.5 26 11066.5 27 14586.5 28 17521.5 29 20919.5 30 25512.5 31 30141.5 32 34548.5 33 38191.0 34 42430.0 35 46951.0 36 51609.0 37 57383.5 38 60376.5 39 61312.0 40 62817.5 41 65626.5 42 68644.5 43 71770.0 44 75598.0 45 78776.0 46 79495.5 47 80021.5 48 83751.0 49 85550.0 50 83393.5 51 80163.5 52 76435.0 53 74578.5 54 71938.0 55 64794.0 56 57563.5 57 51770.5 58 47279.5 59 42846.0 60 36863.0 61 29886.0 62 23922.0 63 18489.5 64 13511.5 65 10450.0 66 8202.0 67 6777.5 68 5204.0 69 3488.5 70 2538.5 71 2105.5 72 1812.5 73 1496.5 74 1109.5 75 894.0 76 635.5 77 362.0 78 237.5 79 124.5 80 67.5 81 49.5 82 25.5 83 18.0 84 16.0 85 10.5 86 11.0 87 11.5 88 10.5 89 7.0 90 4.5 91 5.5 92 5.0 93 5.5 94 4.5 95 1.0 96 0.5 97 0.5 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06473501393822832 2 0.05167526511759881 3 0.04693053622952837 4 0.046977513743271644 5 0.045803075899689856 6 0.004321931264380991 7 4.227976236894448E-4 8 0.0 9 7.98617733635618E-4 10-11 0.0036407573151035527 12-13 0.0023488756871635824 14-15 0.007798267281383093 16-17 0.0029360946089544778 18-19 0.004791706401813708 20-21 0.009818300372343774 22-23 0.016395152296401806 24-25 0.003335403475772287 26-27 0.016324686025786895 28-29 0.007492913442051827 30-31 0.006905694520260932 32-33 0.002677718283366484 34-35 0.018297741603004306 36-37 0.015995843429583996 38-39 0.02976025495636259 40-41 0.006248009327855129 42-43 0.0014797916829130569 44-45 2.8186508245962985E-4 46-47 0.002301898173420311 48-49 0.0029830721226977493 50-51 0.0017616567653726866 52-53 0.0017146792516294152 54-55 0.005496369107962783 56-57 0.002654229526494848 58-59 0.008033154850099452 60-61 0.016230730998300354 62-63 0.01219066481637899 64-65 0.0024193419577784895 66-67 0.0038051786132050035 68-69 0.009959232913573588 70-71 0.002301898173420311 72-73 0.0031240046639275645 74-75 0.0012918816279399701 76-77 3.99308866817809E-4 78-79 0.00286562833833957 80-81 0.002677718283366484 82-83 0.0015737467103996 84-85 0.011321580812128466 86-87 0.006459408139699851 88-89 0.001973055577217409 90-91 8.690840042505254E-4 92-93 0.002207943145933767 94-95 0.0 96-97 0.0017381680085010508 98-99 0.025156458609521967 100-101 0.014797916829130568 102-103 0.006365453112213307 104-105 0.013787900283650229 106-107 0.03349496729895268 108-109 0.0014797916829130569 110-111 0.001973055577217409 112-113 0.01653608483763162 114-115 0.03288425962029015 116-117 0.05141688879201082 118-119 0.3583444748336761 120-121 0.12016848015528887 122-123 0.019754044529045726 124-125 0.31867196447748325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2128678.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.20009995289548 #Duplication Level Percentage of deduplicated Percentage of total 1 84.63144267648623 61.10398620400049 2 9.701216247086155 14.008575654085481 3 2.7499747986197995 5.956453659848796 4 1.111857567403087 3.2110490999954444 5 0.5544855265320416 2.001695521902364 6 0.3100139206953493 1.3429821636595942 7 0.19038025496137573 0.9621831406088337 8 0.13844487850855494 0.7996587253027311 9 0.09459237961093996 0.6146621336422887 >10 0.4643866932618977 6.055998462342569 >50 0.038109686804262306 1.8544932218236485 >100 0.014959375879967474 1.7283051257632638 >500 0.0 0.0 >1k 1.359941502980923E-4 0.35995688702451945 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3666 0.17221956538283387 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.046627061490748E-5 0.0 18-19 0.0 0.0 0.0 9.395502748654329E-5 0.0 20-21 0.0 0.0 0.0 2.1139881184472237E-4 0.0 22-23 0.0 0.0 0.0 4.932638943043522E-4 0.0 24-25 0.0 0.0 0.0 8.455952473788896E-4 0.0 26-27 0.0 0.0 0.0 0.0019025893066025017 0.0 28-29 0.0 0.0 0.0 0.0047212401311988 0.0 30-31 0.0 0.0 0.0 0.013811389040521864 0.0 32-33 0.0 0.0 0.0 0.031451445451120365 0.0 34-35 0.0 0.0 0.0 0.060530526458205516 0.0 36-37 0.0 0.0 0.0 0.10325657520771107 0.0 38-39 0.0 0.0 0.0 0.16209591117115882 0.0 40-41 0.0 0.0 0.0 0.2227438814137225 0.0 42-43 0.0 0.0 0.0 0.2836502279818742 0.0 44-45 0.0 0.0 0.0 0.35963635646161607 0.0 46-47 0.0 0.0 0.0 0.45276927745765216 0.0 48-49 0.0 0.0 0.0 0.553136735570152 0.0 50-51 0.0 0.0 0.0 0.6590945178181011 0.0 52-53 0.0 0.0 0.0 0.7658039402859427 0.0 54-55 0.0 0.0 0.0 0.8791841697053289 0.0 56-57 0.0 0.0 0.0 1.0110970282964358 0.0 58-59 0.0 0.0 0.0 1.1687065869051119 0.0 60-61 0.0 0.0 0.0 1.3436978255987988 0.0 62-63 0.0 0.0 0.0 1.517373693907674 0.0 64-65 0.0 0.0 0.0 1.6941970556373485 0.0 66-67 0.0 0.0 0.0 1.9044214296384894 0.0 68-69 0.0 0.0 0.0 2.1506775566807192 0.0 70-71 0.0 0.0 0.0 2.4232880689329246 0.0 72-73 0.0 0.0 0.0 2.7009721526694035 0.0 74-75 0.0 0.0 0.0 2.9786327476490104 0.0 76-77 0.0 0.0 0.0 3.2818256213480854 0.0 78-79 0.0 0.0 0.0 3.6319960087904324 0.0 80-81 4.6977513743271646E-5 0.0 0.0 4.024070338491778 0.0 82-83 4.6977513743271646E-5 0.0 0.0 4.423073851470255 0.0 84-85 4.6977513743271646E-5 0.0 0.0 4.827080469662391 0.0 86-87 4.6977513743271646E-5 0.0 0.0 5.246589667389808 0.0 88-89 4.6977513743271646E-5 0.0 0.0 5.71157309842071 0.0 90-91 4.6977513743271646E-5 0.0 0.0 6.224872902336568 0.0 92-93 4.6977513743271646E-5 0.0 0.0 6.771667673551377 0.0 94-95 4.6977513743271646E-5 0.0 0.0 7.317828248330654 0.0 96-97 4.6977513743271646E-5 0.0 0.0 7.87230384304249 0.0 98-99 4.6977513743271646E-5 0.0 0.0 8.45557665367895 0.0 100-101 4.6977513743271646E-5 0.0 0.0 9.104970314909066 0.0 102-103 4.6977513743271646E-5 0.0 0.0 9.788328718575567 0.0 104-105 1.4093254122981492E-4 0.0 0.0 10.462972793442692 0.0 106-107 1.4093254122981492E-4 0.0 0.0 11.132190965472468 0.0 108-109 1.4093254122981492E-4 0.0 0.0 11.817945222339874 0.0 110-111 1.4093254122981492E-4 0.0 0.0 12.557817575039532 0.0 112-113 1.4093254122981492E-4 4.6977513743271646E-5 0.0 13.348895417719355 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2425 0.0 59.133045 1 GTATCAA 4070 0.0 46.4898 1 ATCAACG 4575 0.0 38.49055 3 GTACATG 10650 0.0 38.04718 1 TCAACGC 4625 0.0 37.686775 4 CAACGCA 4770 0.0 36.4173 5 TACATGG 10720 0.0 36.240345 2 AACGCAG 4950 0.0 34.962173 6 ACATGGG 10870 0.0 34.58924 3 TATCAAC 5480 0.0 33.655426 2 ACCGTAC 100 4.4547138E-4 29.733078 8 CATGGGA 6590 0.0 28.97695 4 CATGGGG 4740 0.0 28.865734 4 ACGCAGA 6135 0.0 27.819374 7 CGCAGAG 6195 0.0 27.4533 8 CATACCG 115 0.0010060901 25.865177 5 ATGGGAG 3230 0.0 23.759127 5 GCAGAGT 6875 0.0 23.702745 9 GTACCGT 205 2.2739932E-6 23.208483 6 GAGTACA 7840 0.0 22.540592 1 >>END_MODULE