FastQCFastQC Report
Thu 26 May 2016
SRR1364173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128678
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96080.45135995204535395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88270.4146705138118588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49860.23422988352395244No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23140.10870596680193059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49150.077.2817151
GTATCAA88500.051.5978551
ATCAACG110850.040.8530433
TCAACGC112400.040.2367364
CAACGCA113650.039.7857675
TATCAAC115750.039.074052
AACGCAG117850.038.6184736
TGGTATC21050.037.883362
GTGGTAT21400.037.001341
GTACATG104250.034.8364071
TACATGG104150.034.5122682
ACATGGG105550.032.7561873
ACGCAGA140350.032.38357
CGCAGAG140650.032.018358
GCAGAGT154700.028.9950499
GAGTACT103600.027.79287712-13
CAGAGTA150250.027.29999510-11
CATGGGA65800.027.1313114
GTACTTT108950.026.42841514-15
ATGGGAG29100.025.3520055
Produced by FastQC (version 0.11.5)