FastQCFastQC Report
Thu 26 May 2016
SRR1364172_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364172_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057623
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.045.861521
GTATCAA27950.041.0831221
ATCAACG29800.037.5298423
CAACGCA30800.036.3131075
TCAACGC31050.035.827394
AACGCAG33100.033.598676
GTACATG63200.032.383911
ACATGGG61600.031.8689963
TATCAAC35900.031.817282
TACATGG64350.031.4330582
ACGCAGA40150.027.252677
CGCAGAG41850.025.861448
CATGGGG40350.024.178844
GAGTACA47700.020.9545751
GTGTAGC14750.020.5715071
CATGGGA27500.020.1180294
AGTACAT47750.019.8097952
TAGCCCT17350.019.2009944
GCAGAGT52000.019.1008769
GTAGCCC14400.018.5902483
Produced by FastQC (version 0.11.5)