FastQCFastQC Report
Thu 26 May 2016
SRR1364172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057623
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56140.27283909637479753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53430.25966855930362365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28270.13739154354320493No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.071.5713041
GTATCAA63650.049.6658671
ATCAACG75150.041.414763
CAACGCA77250.040.2781525
TCAACGC78000.039.977824
AACGCAG79550.039.486586
TATCAAC81200.038.3309172
ACGCAGA93750.033.2510387
CGCAGAG95900.032.381518
TGGTATC16500.030.6576252
GTGGTAT17800.030.1012441
GTACATG60300.029.9148771
GAGTACT76100.028.2995612-13
GCAGAGT108500.028.182449
TACATGG61900.027.7850232
ACATGGG60300.027.7313753
CAGAGTA103200.027.1510410-11
GTACTTT82750.025.70181814-15
CATGGGG38100.024.9906434
AGAGTAC99550.024.3518310-11
Produced by FastQC (version 0.11.5)