FastQCFastQC Report
Thu 26 May 2016
SRR1364171_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364171_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502162
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25250.052.308521
GTATCAA41100.047.914511
ATCAACG50650.037.466983
CAACGCA50850.037.2041175
GTACATG102750.035.2048571
TCAACGC54150.035.0438884
AACGCAG54450.034.6246
TATCAAC58100.033.175312
TACATGG105100.032.433812
ACATGGG103050.031.866073
ACGCAGA63150.029.6650057
CGCAGAG64550.028.7452168
CATGGGG58950.026.3376354
GGTAAGG15350.025.9659293
GTAAGGT15550.025.2483844
TAAGGTG16600.024.3700055
AATACCG2001.8295577E-623.7965955
CATGGGA51800.023.4272064
GCAGAGT76650.023.1226379
AGGTAAG15450.023.1030272
Produced by FastQC (version 0.11.5)