FastQCFastQC Report
Thu 26 May 2016
SRR1364171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502162
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58330.23311839920836464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53880.21533377934762019No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31550.12609095654078353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41350.072.71261
GTATCAA80100.057.085061
ATCAACG103900.043.8114743
CAACGCA106300.042.7043845
TATCAAC108900.041.6368372
TCAACGC109200.041.576114
AACGCAG109800.041.4506766
ACGCAGA127800.035.564587
CGCAGAG129350.034.954448
GTACATG101000.032.1858561
GCAGAGT146150.030.6921779
TACATGG105500.029.7805082
ACATGGG101950.029.6495743
GTGGTAT19500.027.1737251
AGAGTAC137150.026.76360110-11
GAGTACT94200.026.30384812-13
CAGAGTA142700.025.70184510-11
AGTACTT100250.024.83512112-13
CATGGGG55150.024.707684
TAAGGTG16250.024.530295
Produced by FastQC (version 0.11.5)