##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364170_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1369068 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.411897728965982 31.0 31.0 34.0 26.0 34.0 2 30.520815620553545 31.0 31.0 34.0 26.0 34.0 3 30.679854470340405 33.0 31.0 34.0 26.0 34.0 4 33.98318198949943 37.0 35.0 37.0 30.0 37.0 5 34.10518688626131 37.0 35.0 37.0 30.0 37.0 6 33.919626344345204 37.0 35.0 37.0 29.0 37.0 7 33.887193331521885 37.0 35.0 37.0 29.0 37.0 8 33.758654792895605 37.0 35.0 37.0 28.0 37.0 9 35.138082257418915 39.0 37.0 39.0 27.0 39.0 10-11 35.15520083735797 39.0 35.0 39.0 27.0 39.0 12-13 35.06347931585575 39.0 35.0 39.0 27.0 39.0 14-15 36.26783403015774 40.0 37.0 41.0 27.0 41.0 16-17 36.33163619338119 40.0 37.0 41.0 27.0 41.0 18-19 36.290565552624116 40.0 37.0 41.0 27.0 41.0 20-21 36.298605693800454 40.0 37.0 41.0 27.0 41.0 22-23 36.2879261658296 40.0 37.0 41.0 27.0 41.0 24-25 36.262840487105095 40.0 36.5 41.0 27.0 41.0 26-27 36.11157480855589 40.0 36.0 41.0 27.0 41.0 28-29 36.014999985391526 40.0 36.0 41.0 27.0 41.0 30-31 36.049260153622754 40.0 36.0 41.0 27.0 41.0 32-33 35.78618556565488 40.0 35.5 41.0 26.5 41.0 34-35 35.84197205690295 40.0 35.5 41.0 27.0 41.0 36-37 35.820708321281344 40.0 35.5 41.0 27.0 41.0 38-39 35.69129729129598 40.0 35.0 41.0 26.5 41.0 40-41 35.43185291015494 40.0 35.0 41.0 25.0 41.0 42-43 35.22985308253498 39.0 35.0 41.0 25.0 41.0 44-45 35.016665717115586 39.0 35.0 41.0 25.0 41.0 46-47 34.768893144825526 39.0 34.0 41.0 24.0 41.0 48-49 34.520691813701 39.0 33.5 41.0 23.5 41.0 50-51 34.39788929403068 38.5 33.5 41.0 23.0 41.0 52-53 34.24310041575729 38.0 33.0 40.5 23.0 41.0 54-55 33.9602762609308 38.0 33.0 40.0 22.0 41.0 56-57 33.60396926960531 38.0 33.0 40.0 20.0 41.0 58-59 33.2747332491885 37.5 32.0 40.0 18.0 41.0 60-61 33.01355374605206 37.0 32.0 40.0 17.0 41.0 62-63 32.530335965781106 36.0 31.0 40.0 13.0 41.0 64-65 32.28164342457789 36.0 31.0 39.0 8.5 41.0 66-67 31.89629331778991 35.0 31.0 39.0 8.0 41.0 68-69 31.502342469475586 35.0 30.5 39.0 6.5 40.5 70-71 31.03376932336451 35.0 30.0 38.0 2.0 40.0 72-73 30.843478556214883 35.0 30.0 37.0 2.0 39.5 74-75 30.53455562470235 35.0 30.0 37.0 2.0 39.0 76-77 30.001131061422807 35.0 29.5 36.0 2.0 39.0 78-79 29.673972001390727 35.0 29.0 36.0 2.0 38.0 80-81 29.45996363949782 35.0 29.0 35.5 2.0 37.0 82-83 29.24032334405596 34.0 29.0 35.0 2.0 37.0 84-85 28.95291614441357 34.0 29.0 35.0 2.0 36.0 86-87 28.608349621786502 34.0 29.0 35.0 2.0 36.0 88-89 28.31484520856524 34.0 28.0 35.0 2.0 36.0 90-91 28.177097850508524 34.0 28.0 35.0 2.0 35.5 92-93 27.875236292134502 34.0 27.0 35.0 2.0 35.0 94-95 27.79764628199622 34.0 27.0 35.0 2.0 35.0 96-97 27.723665296391413 34.0 27.0 35.0 2.0 35.0 98-99 27.515658097333368 34.0 26.5 35.0 2.0 35.0 100-101 27.109634802654067 34.0 25.0 35.0 2.0 35.0 102-103 25.93325459363596 32.5 23.5 34.5 2.0 35.0 104-105 26.693578405163223 34.0 24.5 35.0 2.0 35.0 106-107 26.671536402866767 34.0 24.5 35.0 2.0 35.0 108-109 26.62494777469052 34.0 24.5 35.0 2.0 35.0 110-111 26.659172517362176 34.0 25.0 35.0 2.0 35.0 112-113 26.5642951993619 34.0 25.0 35.0 2.0 35.0 114-115 26.375985341853 34.0 24.5 35.0 2.0 35.0 116-117 26.071467231722604 34.0 23.5 35.0 2.0 35.0 118-119 25.83830642451653 34.0 20.0 35.0 2.0 35.0 120-121 25.662679647760374 34.0 19.5 35.0 2.0 35.0 122-123 25.238406711719215 34.0 7.5 35.0 2.0 35.0 124-125 24.242338583620388 32.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18721953909290434 1101 2 0.3406034099832418 1101 3 0.31382999557781943 1101 4 0.4115837680528358 1101 5 0.246548005974482 1101 6 0.3323878634259074 1101 7 0.30846071812877796 1101 8 0.3731450926698159 1101 9 0.4671142517604565 1101 10-11 0.4225966350594845 1101 12-13 0.4904081312009936 1101 14-15 0.47728016286431085 1101 16-17 0.588803161539559 1101 18-19 0.4559390567342021 1101 20-21 0.4675802100222839 1101 22-23 0.49665118180173806 1101 24-25 0.577326992183842 1101 26-27 0.587524298063272 1101 28-29 0.6937063373067218 1101 30-31 0.7096072429399101 1101 32-33 0.7000778555438387 1101 34-35 0.62823066367244 1101 36-37 0.7293935090052344 1101 38-39 0.6866425412324659 1101 40-41 0.6182363493534737 1101 42-43 0.762560417349583 1101 44-45 0.7060479470305907 1101 46-47 0.8531217046285988 1101 48-49 0.7245336593790128 1101 50-51 0.7987528441108438 1101 52-53 0.8060695934374564 1101 54-55 0.7696930930473727 1101 56-57 0.736299956326711 1101 58-59 0.8606467511726308 1101 60-61 0.8522601905225571 1101 62-63 0.7383272433931651 1101 64-65 0.6166580736380496 1101 66-67 0.6990139769872421 1101 68-69 0.6435287503253839 1101 70-71 0.6906154300666607 1101 72-73 0.7098102046786288 1101 74-75 0.6942082863705608 1101 76-77 0.6539166493805872 1101 78-79 0.542770824628267 1101 80-81 0.5426653471609058 1101 82-83 0.500145820684331 1101 84-85 0.4742750052457225 1101 86-87 0.3406288817377501 1101 88-89 0.500057535744773 1101 90-91 0.520621835308674 1101 92-93 0.47752924133573416 1101 94-95 0.5847216969437952 1101 96-97 0.5125415161756521 1101 98-99 0.5081719325113063 1101 100-101 0.45250682459585434 1101 102-103 0.5749253931344995 1101 104-105 0.5479980783813581 1101 106-107 0.5736295771748559 1101 108-109 0.4214835847548457 1101 110-111 0.3359505602423347 1101 112-113 0.4609701010875682 1101 114-115 0.509606884842075 1101 116-117 0.41074807681903636 1101 118-119 0.4610216745123381 1101 120-121 0.43885721134985545 1101 122-123 0.3204597206401161 1101 124-125 0.3733063923590194 1102 1 0.5293870982438165 1102 2 0.6129293105085942 1102 3 0.5420242435740477 1102 4 0.6220965550933997 1102 5 0.528620130544553 1102 6 0.6063798541704486 1102 7 0.654182513361171 1102 8 0.6930012185569723 1102 9 0.7747149248357843 1102 10-11 0.736134156407104 1102 12-13 0.8536743409264957 1102 14-15 0.9464545779271276 1102 16-17 0.9276183533754505 1102 18-19 0.8884683467707148 1102 20-21 0.9095744536222554 1102 22-23 0.9266859346313794 1102 24-25 1.0236005171848461 1102 26-27 1.0152786067277972 1102 28-29 1.1151972188945365 1102 30-31 1.0092733520001858 1102 32-33 1.1841499612728228 1102 34-35 1.0599889271703873 1102 36-37 1.0631740938055927 1102 38-39 1.1236661029062915 1102 40-41 1.0648330886794355 1102 42-43 1.0558212304500643 1102 44-45 1.1848359134988442 1102 46-47 1.0912052791951012 1102 48-49 0.9816406736008858 1102 50-51 1.0260178228854642 1102 52-53 1.1314885496323797 1102 54-55 1.1115003164501545 1102 56-57 1.1298747426486173 1102 58-59 1.2418479914543354 1102 60-61 1.2626078040524291 1102 62-63 1.3489933834066505 1102 64-65 1.2245255275031752 1102 66-67 1.244136917309003 1102 68-69 1.2336930984171168 1102 70-71 1.2098863832595796 1102 72-73 1.1160936391930711 1102 74-75 1.00226237912522 1102 76-77 1.006521160205466 1102 78-79 0.9586082592620393 1102 80-81 1.0900380027355574 1102 82-83 1.1174347141788203 1102 84-85 1.0708539576903782 1102 86-87 1.1508260357470164 1102 88-89 1.2417476079734158 1102 90-91 1.1969205580176983 1102 92-93 1.232385755259525 1102 94-95 1.3495274251719103 1102 96-97 1.2323593318569088 1102 98-99 1.2087486267173624 1102 100-101 1.1599766642806486 1102 102-103 0.9989972790508368 1102 104-105 1.145036955165601 1102 106-107 1.069581659810055 1102 108-109 0.9307980339379718 1102 110-111 1.0685742575719672 1102 112-113 1.1212087054835536 1102 114-115 1.06777279046349 1102 116-117 0.9590759081069926 1102 118-119 0.9770269518104904 1102 120-121 1.0552973588306926 1102 122-123 0.9206994510152171 1102 124-125 0.8259044658132311 1103 1 0.026662285927418594 1103 2 -0.015653451613768965 1103 3 -0.03568923519141265 1103 4 0.02874096266602777 1103 5 -0.02470830669477664 1103 6 -0.03605885398607711 1103 7 -0.08033154930883057 1103 8 -0.04224272479340385 1103 9 0.018752343687559403 1103 10-11 -0.02296985838997756 1103 12-13 0.15275054318958325 1103 14-15 -0.007262260102827156 1103 16-17 -0.11354487695857074 1103 18-19 -0.07298760651691794 1103 20-21 0.03384196622516811 1103 22-23 0.12177057358978516 1103 24-25 1.5049361105923253E-4 1103 26-27 0.21941013938655374 1103 28-29 0.26421221998256783 1103 30-31 0.07027222317851312 1103 32-33 0.07207247605177258 1103 34-35 -0.04142277993439336 1103 36-37 0.017681220342424808 1103 38-39 0.02856072354966699 1103 40-41 -0.006576329048051832 1103 42-43 0.08535555750913915 1103 44-45 -0.17081703503777135 1103 46-47 -0.1618569329327002 1103 48-49 -0.1313147084757773 1103 50-51 -0.12239185324698099 1103 52-53 -0.09613686744229 1103 54-55 -0.16796906936205147 1103 56-57 -0.15350233819133052 1103 58-59 -0.09711165657090959 1103 60-61 -0.11615176146455752 1103 62-63 -0.2265615072472471 1103 64-65 -0.18245151978945984 1103 66-67 -0.12017951089974943 1103 68-69 -0.04075133091592775 1103 70-71 -0.2621356128960848 1103 72-73 -0.28927880395783845 1103 74-75 -0.37609586535307216 1103 76-77 -0.3277946786586412 1103 78-79 -0.269901401456778 1103 80-81 -0.22823553478302827 1103 82-83 -0.10951850998975488 1103 84-85 -0.12002072043677714 1103 86-87 -0.11641614863625449 1103 88-89 -0.03321023121709743 1103 90-91 0.09304210355137243 1103 92-93 -0.015755542386724386 1103 94-95 -0.02036091668425044 1103 96-97 0.06635885131434804 1103 98-99 0.1385031646711532 1103 100-101 -0.10469099067165999 1103 102-103 -0.0188383300595234 1103 104-105 -0.07731892740067892 1103 106-107 -0.05606718598111371 1103 108-109 -0.04737094023966648 1103 110-111 0.08040433137605163 1103 112-113 0.08889131165625841 1103 114-115 0.052480430183507565 1103 116-117 -0.09197391871635219 1103 118-119 -0.010444374305638604 1103 120-121 0.04087314855252089 1103 122-123 -0.05868719987514481 1103 124-125 -0.08500917706204447 1104 1 -0.06637650314238996 1104 2 0.05085178888781883 1104 3 0.12988838973840444 1104 4 -0.002742988297853799 1104 5 0.030312280990045792 1104 6 0.05065039175601527 1104 7 0.025838907363450403 1104 8 -0.07289147081623781 1104 9 0.22995742510249073 1104 10-11 0.1657490261318273 1104 12-13 0.16096982542802607 1104 14-15 0.32135520667613804 1104 16-17 0.18976435942548164 1104 18-19 0.28824671876850516 1104 20-21 0.25441385935636873 1104 22-23 0.2175021382538347 1104 24-25 0.40358723327042156 1104 26-27 0.33630022922372405 1104 28-29 0.45791016589310374 1104 30-31 0.5065129821154386 1104 32-33 0.3487218303471167 1104 34-35 0.2005527020046003 1104 36-37 0.2959939659909381 1104 38-39 0.341385330512054 1104 40-41 0.3770687439446476 1104 42-43 0.31620901062536433 1104 44-45 0.2529441946084958 1104 46-47 0.3256319543965134 1104 48-49 0.2976870227613446 1104 50-51 0.333831242392165 1104 52-53 0.5118823867220357 1104 54-55 0.5382873642894523 1104 56-57 0.5686142161788723 1104 58-59 0.30571505595014514 1104 60-61 0.4118498294019943 1104 62-63 0.4275326346206185 1104 64-65 0.3250974929374735 1104 66-67 0.38146574925371723 1104 68-69 0.3979476677739484 1104 70-71 0.40263839047280925 1104 72-73 0.3421273583105524 1104 74-75 0.4376072274786722 1104 76-77 0.3244740608135679 1104 78-79 0.4371340889044397 1104 80-81 0.42444437350819086 1104 82-83 0.4203231052077001 1104 84-85 0.4058753117548761 1104 86-87 0.3695666793629968 1104 88-89 0.3574948969880296 1104 90-91 0.40180532178083084 1104 92-93 0.3361441394399627 1104 94-95 0.2382192349159702 1104 96-97 0.27850441560407546 1104 98-99 0.25084349313830856 1104 100-101 0.28176675778426485 1104 102-103 0.1731593772210296 1104 104-105 0.1873369307314583 1104 106-107 0.2303724312751072 1104 108-109 0.34391848049774865 1104 110-111 0.3628539149433365 1104 112-113 0.41284087182844686 1104 114-115 0.4669795411698452 1104 116-117 0.5029709858316558 1104 118-119 0.3875418927727843 1104 120-121 0.4529929782362174 1104 122-123 0.4164362092583005 1104 124-125 0.42054306418818754 1105 1 0.17885398094186655 1105 2 0.17947027273605798 1105 3 0.15025415154548583 1105 4 0.1926608664664542 1105 5 0.03382620444315876 1105 6 -0.04243664761423105 1105 7 0.018781210850647767 1105 8 0.12581458344757124 1105 9 0.1748606047797736 1105 10-11 0.1650701211264618 1105 12-13 0.24026391694136606 1105 14-15 0.17497065350044494 1105 16-17 0.19345633925270533 1105 18-19 0.28345099767489756 1105 20-21 0.2770120940645171 1105 22-23 0.040976424206121465 1105 24-25 0.12115025585546846 1105 26-27 0.2505312027027031 1105 28-29 0.3051131536304652 1105 30-31 0.1412485527809153 1105 32-33 0.14349745501954914 1105 34-35 0.19791205308803228 1105 36-37 0.21178579622303317 1105 38-39 0.26016975734754766 1105 40-41 0.22544489063811568 1105 42-43 0.25876899618648963 1105 44-45 0.18621435784419305 1105 46-47 0.23300446013215037 1105 48-49 0.1497738920582563 1105 50-51 0.17883716025021812 1105 52-53 0.2428069262224426 1105 54-55 0.33212203217787106 1105 56-57 0.3666719092207913 1105 58-59 0.32587256468809755 1105 60-61 0.19184642793519657 1105 62-63 0.21181681675875552 1105 64-65 0.27315848331964787 1105 66-67 0.3012039751458211 1105 68-69 0.2045628265148487 1105 70-71 0.11894772958169142 1105 72-73 0.08877326908799787 1105 74-75 0.1769868415857303 1105 76-77 0.10225797048726903 1105 78-79 0.14585642161648593 1105 80-81 0.25022540928953774 1105 82-83 0.13342083490216083 1105 84-85 0.09153465832616092 1105 86-87 0.11860090575491 1105 88-89 0.05578305675978612 1105 90-91 0.01886818991151884 1105 92-93 -0.022156654351316973 1105 94-95 0.10238200551480503 1105 96-97 0.12251557796857782 1105 98-99 0.059287460914617895 1105 100-101 0.06233848187671143 1105 102-103 -0.17399328269129555 1105 104-105 0.1135139944325978 1105 106-107 0.14556726937570375 1105 108-109 0.07506758622436038 1105 110-111 0.1776277347558377 1105 112-113 0.10601017116099598 1105 114-115 0.1100115670209938 1105 116-117 0.035119359201640776 1105 118-119 0.08215056790146491 1105 120-121 0.10597360028813796 1105 122-123 0.05298739959813403 1105 124-125 0.00547248375217535 1106 1 -0.047416165657963916 1106 2 -0.06905704894065678 1106 3 -0.08173377521700331 1106 4 -0.07893446033980211 1106 5 -0.15037658481222138 1106 6 -0.07928898337609525 1106 7 -0.00953939091269973 1106 8 0.04900656427242467 1106 9 -0.042028084294813084 1106 10-11 0.1454006804981205 1106 12-13 0.11804051546315009 1106 14-15 0.08151875440022849 1106 16-17 0.14763367415759632 1106 18-19 0.11142516186072982 1106 20-21 0.10024455047086178 1106 22-23 0.0037634997948146065 1106 24-25 0.00290003150384166 1106 26-27 0.09714456992828957 1106 28-29 0.20774938406617594 1106 30-31 0.0266053136093376 1106 32-33 -0.3656763509068526 1106 34-35 -0.01803660049460376 1106 36-37 0.021306984596648704 1106 38-39 -0.09897686709443576 1106 40-41 -0.11189633829019385 1106 42-43 -0.18284080250791845 1106 44-45 -0.1807461193545592 1106 46-47 -0.08084642888037763 1106 48-49 -0.02136607346938746 1106 50-51 -0.025566174304856304 1106 52-53 -0.019746546810978316 1106 54-55 0.04319570740461387 1106 56-57 0.02843371328030031 1106 58-59 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2216 90-91 -2.460627768243498 2216 92-93 -2.4145019811331636 2216 94-95 -2.319353081926419 2216 96-97 -2.2586731999677028 2216 98-99 -2.26426689705724 2216 100-101 -2.285591987822656 2216 102-103 -2.2924078932124203 2216 104-105 -2.238450218948639 2216 106-107 -2.331280267652531 2216 108-109 -2.3770403372007287 2216 110-111 -2.397445265015211 2216 112-113 -2.514083522151907 2216 114-115 -2.4326798262267495 2216 116-117 -2.3189142083649728 2216 118-119 -2.243680960250554 2216 120-121 -2.4017966045338994 2216 122-123 -2.3995104429900564 2216 124-125 -2.513749086843621 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40931.0 3 7153.0 4 5680.0 5 4862.0 6 5778.0 7 4626.0 8 4331.0 9 4664.0 10 5709.0 11 6552.0 12 7317.0 13 7854.0 14 8325.0 15 8332.0 16 8279.0 17 8579.0 18 8960.0 19 9101.0 20 9713.0 21 10551.0 22 12503.0 23 16476.0 24 19325.0 25 18488.0 26 21628.0 27 23010.0 28 26406.0 29 32390.0 30 39095.0 31 46102.0 32 57950.0 33 71079.0 34 88126.0 35 114431.0 36 178625.0 37 292057.0 38 133522.0 39 558.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.119536908987875 18.01223497493988 11.07658677377775 22.79164134229449 2 13.595441127035132 22.693810085126557 37.65951137736506 26.051237410473256 3 21.21574149972371 30.76618250212264 26.007436755654805 22.01063924249885 4 12.917105145354768 19.004657047288184 32.61672339370029 35.46151441365676 5 12.06697391789347 41.61057374763323 31.999970644787247 14.322481689686045 6 31.332363587258065 32.058792445961856 19.529545941598 17.079298025182084 7 27.12111161335392 33.9301543320399 20.7786165884929 18.170117466113286 8 28.87890790153615 33.87241919951144 20.29165394372152 16.957018955230893 9 30.422854911161636 17.275158512780493 18.428005429480258 33.87398114657761 10-11 23.879174749886666 30.372449082596592 23.811817132327434 21.93655903518931 12-13 26.88346974988382 27.459811016153413 24.059944483884397 21.59677475007837 14-15 26.81552539399733 26.55243848063546 22.56165698149144 24.07037914387577 16-17 23.036331414843993 30.238171344352992 27.178521531351574 19.546975709451438 18-19 24.714252271121115 28.978800912631463 27.39233686052135 18.91460995572607 20-21 22.894799130283207 29.25852056403152 26.989256979293767 20.8574233263915 22-23 21.474348269239027 30.492451422306704 22.999625993695112 25.033574314759154 24-25 22.13362466792737 31.611038958417666 22.184011264872396 24.07132510878257 26-27 23.8459358915259 28.26352329415495 23.162540849553263 24.727999964765885 28-29 23.236912290334054 27.849003450223524 25.93620481284345 22.977879446598973 30-31 25.06861146256477 29.43372097846167 24.544826216717887 20.952841342255667 32-33 20.35532152769978 29.89100233367175 26.38366767457208 23.37000846405639 34-35 19.15413079877941 30.92441335955387 26.979294438559748 22.942161403106972 36-37 24.27258443149272 28.897198634260373 24.756763855958273 22.073453078288637 38-39 25.782357904881557 27.638196851828827 24.235079778777955 22.344365464511657 40-41 21.965388503831846 30.533664489314866 24.487635372475307 23.01331163437798 42-43 24.836072547615974 28.94746106814688 23.881479351742104 22.33498703249504 44-45 26.02221114343037 27.509080390575104 23.942039576232542 22.526668889761986 46-47 22.836534933889027 28.91059976813466 28.205685103385527 20.047180194590787 48-49 24.68384545765046 28.483899973695827 27.061273320192253 19.770981248461464 50-51 21.456452243002484 29.5752060143536 27.07321143959694 21.89513030304698 52-53 20.689385767067662 30.642542248481558 23.274160578158547 25.39391140629224 54-55 21.604922874670056 31.230843382686306 22.496416272835713 24.667817469807922 56-57 23.60485555925153 28.14310689631271 23.19351418515276 25.058523359282997 58-59 23.12758632451173 27.503620808471794 25.797393898117104 23.571398968899373 60-61 24.76874462415385 29.29654130208725 24.552188576888582 21.382525496870315 62-63 20.30953522399195 29.818459652544988 26.160289419909393 23.71171570355367 64-65 18.83939622719032 30.41571928218154 27.04684573242267 23.69803875820547 66-67 23.69376295746357 29.10732337867559 24.740667324963283 22.458246338897553 68-69 25.169136527033963 27.701562614628422 24.25207248184286 22.877228376494756 70-71 21.764717751180815 30.10108680698858 24.477571422020453 23.656624019810153 72-73 24.31772600893423 28.88440273947778 24.069589690970503 22.728281560617493 74-75 25.576486152042293 27.369440171459114 23.885961491403748 23.16811218509484 76-77 22.370197281057642 28.68773981497106 28.150774377673272 20.791288526298025 78-79 24.060665283075068 28.320674864043415 27.03850507549231 20.580154777389208 80-81 20.96072704382028 29.829148810820843 26.971773273494698 22.238350871864178 82-83 20.338425973307565 30.441390254397234 23.220648173520253 25.999535598774948 84-85 21.193414581181333 30.847526763076345 22.646639355808055 25.312419299934263 86-87 23.243852396623453 27.613325257953008 23.551558136318633 25.591264209104907 88-89 22.81215279726423 27.347241800375826 25.752745618253332 24.08785978410661 90-91 24.095254619143287 28.914731042264187 24.809983387187113 22.180030951405413 92-93 20.242476400939708 29.099772703594628 26.162014353863377 24.495736541602287 94-95 18.932380555856152 29.773464682610285 27.10691051020427 24.187244251329297 96-97 23.51431963182962 28.266003027012438 25.18174360170472 23.037933739453223 98-99 24.643423457444175 27.389602699873695 24.385718240095912 23.581255602586218 100-101 21.48034221250245 29.216053264015805 24.739720151271566 24.563884372210172 102-103 24.096248635955927 27.9617946696255 24.521599456911602 23.42035723750697 104-105 24.829803799667747 26.78924116614639 24.480980400494424 23.899974633691443 106-107 22.032131635582378 28.024952979435607 28.196060171657138 21.746855213324874 108-109 23.568030119253823 28.146087767646442 26.806773898797783 21.479108214301952 110-111 20.62702797059186 29.329662205375843 27.086829412834668 22.956480411197624 112-113 19.92582984870037 29.828788183821402 23.90819417720674 26.337187790271482 114-115 20.551773555236764 31.03861014055253 23.118705426424814 25.290910877785894 116-117 22.344717149632 28.078271619059354 23.79529953822443 25.78171169308422 118-119 22.051967825074062 27.7927156994928 25.629841601775677 24.52547487365747 120-121 23.120788809905985 29.369704196285255 24.752414583811053 22.757092409997707 122-123 19.852171585112274 29.657878620072538 25.848078890718458 24.641870904096727 124-125 19.033858800163696 30.454071402273293 26.374308207051634 24.13776159051138 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8705.0 1 5596.0 2 4495.5 3 6364.0 4 5488.5 5 4043.5 6 2796.0 7 2194.0 8 2095.0 9 1866.5 10 1478.5 11 1204.0 12 1093.5 13 1059.0 14 1069.5 15 1110.5 16 1107.0 17 1160.0 18 1287.0 19 1367.0 20 1567.0 21 1904.5 22 2296.0 23 2926.0 24 3510.5 25 4128.0 26 5155.0 27 6258.0 28 7308.5 29 8783.0 30 10905.0 31 12998.0 32 15095.5 33 17564.5 34 19682.0 35 21364.5 36 23970.5 37 26373.0 38 26922.0 39 26644.0 40 25988.0 41 26756.5 42 27861.0 43 29164.5 44 30797.5 45 32017.0 46 33763.5 47 38554.0 48 46909.0 49 69143.0 50 149671.5 51 162007.0 52 82405.0 53 46791.5 54 37405.5 55 32265.0 56 27733.5 57 23608.5 58 21591.5 59 20181.0 60 18750.0 61 17470.0 62 15764.5 63 12699.0 64 9761.5 65 7767.5 66 5631.5 67 4342.5 68 3210.5 69 2096.5 70 1705.5 71 1520.5 72 1456.0 73 1507.0 74 1122.5 75 796.0 76 609.5 77 409.0 78 381.5 79 261.0 80 148.0 81 131.5 82 83.0 83 49.5 84 41.0 85 44.0 86 40.0 87 25.0 88 12.5 89 8.5 90 10.0 91 6.0 92 1.5 93 0.5 94 0.5 95 2.0 96 2.5 97 1.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4925978841080209 2 0.48397888198394823 3 0.46579132665433715 4 0.4676173864263864 5 0.47097733640695716 6 0.47565204942340333 7 0.4595827234293695 8 0.4893840189092141 9 0.5021664373135593 10-11 0.5065855019619185 12-13 0.5069872351117695 14-15 0.48138587710763825 16-17 0.48386931839762526 18-19 0.46856693750785205 20-21 0.47882939342676917 22-23 0.49606739767491465 24-25 0.4822623857982219 26-27 0.4931822232350767 28-29 0.4888362009775994 30-31 0.5016916617728265 32-33 0.4993908264600443 34-35 0.49500828300712596 36-37 0.44873592838339654 38-39 0.4120686481606465 40-41 0.4062252568900887 42-43 0.41341993239196295 44-45 0.44581423274811766 46-47 0.45344716259528384 48-49 0.450415903373682 50-51 0.43792565453286464 52-53 0.43186313608966104 54-55 0.43547873443831864 56-57 0.4180581242129683 58-59 0.42127198941177507 60-61 0.43171705130789706 62-63 0.43295877195289056 64-65 0.4459237963344406 66-67 0.4348943953112629 68-69 0.43591698878361046 70-71 0.44778637730193094 72-73 0.44610640231164556 74-75 0.45121936967338366 76-77 0.44102995614534846 78-79 0.434529183356853 80-81 0.44329427026268964 82-83 0.4398977990866779 84-85 0.4379621757283057 86-87 0.44391513058518645 88-89 0.4339813654252382 90-91 0.43558829802464155 92-93 0.44482816047121104 94-95 0.4408473501681436 96-97 0.4146981742323975 98-99 0.387161192869894 100-101 0.3867959809154841 102-103 0.36649019625029583 104-105 0.3691562435174878 106-107 0.3873437988470989 108-109 0.3960358433620536 110-111 0.4116669150107956 112-113 0.40768610470772815 114-115 0.4084530498119888 116-117 0.37996651736801973 118-119 0.4532645566180789 120-121 0.45691667616217746 122-123 0.4136390595646089 124-125 0.40772262590316916 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1369068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.582300732452296 #Duplication Level Percentage of deduplicated Percentage of total 1 81.0072826618426 36.92498319810878 2 11.027941947465726 10.053579326188169 3 3.4793016639613548 4.757837243568245 4 1.5303106166674856 2.790203149720074 5 0.7870443927853095 1.793764710086514 6 0.48272831415519785 1.3202320312735163 7 0.32808822372833224 1.0468511256532653 8 0.21622214776230958 0.7884722371454665 9 0.15670532974571158 0.6428690520162416 >10 0.8773495069488376 7.369435709785778 >50 0.0670330011214527 2.08997065210875 >100 0.030903058858630667 2.6159134547625382 >500 0.003181197234999615 0.9764102049439055 >1k 0.0022722837392854392 3.1406290690897536 >5k 0.0020450553653568954 5.832877977237302 >10k+ 0.0015905986174998075 17.855970858311725 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 54538 3.9835859139210035 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 41978 3.0661734844434316 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 24849 1.8150303710261286 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 14414 1.0528330221727482 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 14378 1.0502034961009972 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 11926 0.8711035536584012 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 10312 0.753213134774898 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 9094 0.6642475026806557 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 8287 0.605302293238904 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 6436 0.4701008277163735 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 6012 0.43913085398241725 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 5459 0.39873841182468656 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 5418 0.3957436737985257 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 5292 0.38654033254739717 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5202 0.37996651736801973 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5115 0.3736118293612881 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 4440 0.32430821551595684 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 3884 0.28369664618558027 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3480 0.25418752026926345 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 3387 0.24739457791724007 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 3385 0.24724849313547612 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3277 0.2393599149202231 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 3099 0.22635836934323203 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2505 0.18297118915934052 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 1687 0.1232225134178872 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.4608478176394453E-4 0.0 12-13 0.0 0.0 0.0 3.652119544098613E-4 0.0 14-15 0.0 0.0 0.0 8.765086905836671E-4 0.0 16-17 0.0 0.0 0.0 0.0017895385766083204 0.0 18-19 0.0 0.0 0.0 0.00273908965807396 0.0 20-21 0.0 0.0 0.0 0.004565149430123266 0.0 22-23 0.0 0.0 0.0 0.007669451042607088 0.0 24-25 0.0 0.0 0.0 0.011358091782146687 0.0 26-27 0.0 0.0 0.0 0.014206745026543604 0.0 28-29 0.0 0.0 0.0 0.019319712388281663 0.0 30-31 0.0 0.0 0.0 0.02797523570779538 0.0 32-33 0.0 0.0 0.0 0.042035895952575036 0.0 34-35 0.0 0.0 0.0 0.06164777790438458 0.0 36-37 0.0 0.0 0.0 0.08998822556658984 0.0 38-39 0.0 0.0 0.0 0.13626058019031925 0.0 40-41 0.0 0.0 0.0 0.21591330744711001 0.0 42-43 0.0 0.0 0.0 0.276575013074588 0.0 44-45 0.0 0.0 0.0 0.3358854344707495 0.0 46-47 0.0 0.0 0.0 0.413456453587404 0.0 48-49 0.0 0.0 0.0 0.4904796547724437 0.0 50-51 0.0 0.0 0.0 0.5798470200165369 0.0 52-53 0.0 0.0 0.0 0.6893375639486132 0.0 54-55 0.0 0.0 0.0 0.7974403024539322 0.0 56-57 0.0 0.0 0.0 0.9259949104062033 0.0 58-59 0.0 0.0 0.0 1.0770465747501219 0.0 60-61 0.0 0.0 0.0 1.2154984266669002 0.0 62-63 0.0 0.0 0.0 1.3543520117335297 0.0 64-65 0.0 0.0 0.0 1.5158122167781294 0.0 66-67 0.0 0.0 0.0 1.689872234249869 0.0 68-69 0.0 0.0 0.0 1.9094741824365187 0.0 70-71 0.0 3.652119544098613E-5 0.0 2.239625789223034 0.0 72-73 0.0 7.304239088197227E-5 0.0 2.503783595847686 0.0 74-75 0.0 7.304239088197227E-5 0.0 2.7580076373123905 0.0 76-77 0.0 7.304239088197227E-5 0.0 3.0399877873122447 0.0 78-79 0.0 7.304239088197227E-5 0.0 3.329016528032209 0.0 80-81 0.0 7.304239088197227E-5 0.0 3.6455822501146766 0.0 82-83 0.0 7.304239088197227E-5 0.0 4.036578168505875 0.0 84-85 0.0 7.304239088197227E-5 0.0 4.402995322365288 0.0 86-87 0.0 7.304239088197227E-5 0.0 4.7664907805894225 0.0 88-89 0.0 7.304239088197227E-5 0.0 5.200837357969071 0.0 90-91 0.0 7.304239088197227E-5 0.0 5.625432776165976 0.0 92-93 7.304239088197227E-5 7.304239088197227E-5 0.0 6.065549702425299 0.0 94-95 7.304239088197227E-5 7.304239088197227E-5 0.0 6.5443425746566275 0.0 96-97 7.304239088197227E-5 7.304239088197227E-5 0.0 7.015465995845348 0.0 98-99 7.304239088197227E-5 7.304239088197227E-5 0.0 7.5492232672153605 0.0 100-101 7.304239088197227E-5 7.304239088197227E-5 0.0 8.319637885042964 0.0 102-103 7.304239088197227E-5 7.304239088197227E-5 0.0 8.979174153511732 0.0 104-105 7.304239088197227E-5 7.304239088197227E-5 0.0 9.57826784352567 0.0 106-107 7.304239088197227E-5 7.304239088197227E-5 0.0 10.205957629569898 0.0 108-109 7.304239088197227E-5 7.304239088197227E-5 0.0 10.786681158277016 0.0 110-111 7.304239088197227E-5 7.304239088197227E-5 0.0 11.416963949197557 0.0 112-113 7.304239088197227E-5 7.304239088197227E-5 0.0 12.17671437795639 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1110 0.0 42.907887 5 AAGTACT 1370 0.0 33.46111 4 GAACAAA 1525 0.0 32.78446 1 CGCAAAA 420 0.0 29.761887 2 GTGTAAG 265 1.8189894E-12 29.198196 1 AAAGTAC 1650 0.0 27.060232 3 CATGGGG 4445 0.0 26.921202 4 ATGGGGC 1050 0.0 26.648863 5 ATGGGAG 2850 0.0 25.902817 5 ATGGGAT 2700 0.0 24.916372 5 AACGGTC 120 0.0012844402 24.803392 7 AAAAAGT 1800 0.0 24.79975 1 CGTATAG 125 0.0016305852 23.807758 1 AAAAGTA 1890 0.0 23.620544 2 CAAGACT 745 0.0 23.174582 4 TGGGAGT 645 0.0 22.150007 6 ACTTGAC 600 0.0 21.826986 8 GGACCGA 165 3.2054388E-4 21.646597 6 CCAAGAC 835 0.0 21.388927 3 ATGGGGG 1650 0.0 21.288166 5 >>END_MODULE