##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364170_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1369068 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11959084574323 33.0 31.0 34.0 30.0 34.0 2 31.93767365828432 33.0 31.0 34.0 30.0 34.0 3 32.16573537618292 34.0 31.0 34.0 30.0 34.0 4 35.640464169785574 37.0 35.0 37.0 33.0 37.0 5 35.772115775111246 37.0 35.0 37.0 35.0 37.0 6 35.905168333494025 37.0 35.0 37.0 35.0 37.0 7 35.88059322108179 37.0 35.0 37.0 35.0 37.0 8 35.927440419321755 37.0 35.0 37.0 35.0 37.0 9 37.591186851200966 39.0 37.0 39.0 35.0 39.0 10-11 37.55541068814698 39.0 37.0 39.0 35.0 39.0 12-13 37.53277339036483 39.0 37.0 39.0 35.0 39.0 14-15 38.802092372329206 40.0 38.0 41.0 34.0 41.0 16-17 38.79699803077714 40.0 38.0 41.0 34.5 41.0 18-19 38.89028959847137 40.0 38.5 41.0 35.0 41.0 20-21 38.95414690869993 40.0 38.5 41.0 35.0 41.0 22-23 38.91543005898903 40.0 38.0 41.0 35.0 41.0 24-25 38.88257266987469 40.0 38.0 41.0 35.0 41.0 26-27 38.70605879328127 40.0 38.0 41.0 34.5 41.0 28-29 38.55426903557749 40.0 38.0 41.0 34.0 41.0 30-31 38.38616964241367 40.0 38.0 41.0 34.0 41.0 32-33 38.09633122679078 40.0 37.0 41.0 34.0 41.0 34-35 37.91384430868299 40.0 37.0 41.0 33.0 41.0 36-37 37.80925125705954 40.0 37.0 41.0 33.0 41.0 38-39 37.71141316574487 40.0 37.0 41.0 33.0 41.0 40-41 37.48818101073139 40.0 36.0 41.0 33.0 41.0 42-43 37.29554266113882 40.0 36.0 41.0 32.5 41.0 44-45 37.018766781489305 40.0 36.0 41.0 31.5 41.0 46-47 36.60767544051866 40.0 35.0 41.0 30.5 41.0 48-49 36.19907594071295 39.5 35.0 41.0 30.0 41.0 50-51 36.023986755953686 39.0 35.0 41.0 29.0 41.0 52-53 35.92946515439701 39.0 35.0 41.0 29.0 41.0 54-55 35.750505088132954 39.0 35.0 41.0 28.0 41.0 56-57 35.488269026812404 39.0 35.0 41.0 28.0 41.0 58-59 35.338000742110694 39.0 35.0 41.0 28.0 41.0 60-61 35.17592552013487 38.0 35.0 40.0 28.0 41.0 62-63 34.85569526130185 38.0 35.0 40.0 28.0 41.0 64-65 34.588594941960515 37.0 34.0 40.0 27.0 41.0 66-67 34.16766077360657 37.0 34.0 39.5 26.0 41.0 68-69 33.855784007806776 36.0 34.0 39.0 26.0 41.0 70-71 33.441884552118665 36.0 34.0 39.0 26.0 40.5 72-73 33.13376179999825 35.0 34.0 38.5 26.0 39.5 74-75 32.81185558350644 35.0 33.5 37.0 25.5 39.0 76-77 32.38654471509085 35.0 33.0 37.0 25.0 39.0 78-79 32.0046999126413 35.0 33.0 36.0 24.0 38.0 80-81 31.78482624676057 35.0 33.0 36.0 24.0 37.0 82-83 31.585062611937463 35.0 33.0 35.5 23.5 37.0 84-85 31.333201126605836 35.0 33.0 35.0 21.5 36.0 86-87 31.08840795343986 35.0 33.0 35.0 20.0 36.0 88-89 30.910009217949728 35.0 32.0 35.0 20.0 36.0 90-91 30.755988380416458 35.0 32.0 35.0 18.5 35.5 92-93 30.59131248411328 35.0 32.0 35.0 18.0 35.0 94-95 30.51732784638893 35.0 32.0 35.0 17.5 35.0 96-97 30.416528251335947 35.0 32.0 35.0 13.0 35.0 98-99 30.321092159045424 35.0 32.0 35.0 8.5 35.0 100-101 30.118696806878837 35.0 32.0 35.0 7.0 35.0 102-103 28.771786719140323 33.5 29.0 34.5 2.0 35.0 104-105 29.67830451080589 34.0 31.0 35.0 2.0 35.0 106-107 29.768798189717387 35.0 31.0 35.0 2.0 35.0 108-109 29.691229727084412 35.0 31.0 35.0 2.0 35.0 110-111 29.701001703348556 35.0 31.0 35.0 2.0 35.0 112-113 29.62591850806534 35.0 31.0 35.0 2.0 35.0 114-115 29.497300353233 35.0 31.0 35.0 2.0 35.0 116-117 29.306761241954383 35.0 31.0 35.0 2.0 35.0 118-119 29.13755196966111 35.0 31.0 35.0 2.0 35.0 120-121 28.961236037946982 34.5 31.0 35.0 2.0 35.0 122-123 28.69241483987647 34.0 30.5 35.0 2.0 35.0 124-125 27.699132913777838 33.5 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32096675107602124 1101 2 0.5971845886873872 1101 3 0.48329938516759796 1101 4 0.5724135803084636 1101 5 0.24361804272366783 1101 6 0.23649032479344356 1101 7 0.26161986294212625 1101 8 0.29005608581036313 1101 9 0.3474948637342763 1101 10-11 0.3435463273370374 1101 12-13 0.29591219563375404 1101 14-15 0.3971095594846048 1101 16-17 0.4690096565105222 1101 18-19 0.33240874533785103 1101 20-21 0.3481838475395236 1101 22-23 0.33006764392828103 1101 24-25 0.3744016682542082 1101 26-27 0.3996114691269028 1101 28-29 0.47558168737855055 1101 30-31 0.44922749638838866 1101 32-33 0.3514333709980022 1101 34-35 0.2804071486932642 1101 36-37 0.16889817023377418 1101 38-39 0.1948573523907342 1101 40-41 0.31858137621706817 1101 42-43 0.3182443774380772 1101 44-45 0.3257996720136873 1101 46-47 0.3439782742681601 1101 48-49 0.3575231078061023 1101 50-51 0.32915291438836647 1101 52-53 0.2845039321876044 1101 54-55 0.3657791266675474 1101 56-57 0.238007551482724 1101 58-59 0.3281738584362017 1101 60-61 0.3331443243064882 1101 62-63 0.30178593751401905 1101 64-65 0.34166035552151897 1101 66-67 0.3011534296697036 1101 68-69 0.4582635381975422 1101 70-71 0.42478900484725557 1101 72-73 0.32690063755084253 1101 74-75 0.34339027372936215 1101 76-77 0.2750953499424824 1101 78-79 0.32489185141884036 1101 80-81 0.3406398574834668 1101 82-83 0.45443551913781377 1101 84-85 0.38057234597847867 1101 86-87 0.3246309166503245 1101 88-89 0.23825091139289256 1101 90-91 0.29643405646407217 1101 92-93 0.2723405592476418 1101 94-95 0.26129440209378885 1101 96-97 0.175208001767583 1101 98-99 0.14195198177909063 1101 100-101 0.24553117708452277 1101 102-103 0.17738404766650362 1101 104-105 0.34866973133516765 1101 106-107 0.23270862239782986 1101 108-109 0.20920375852127648 1101 110-111 0.3114355457213769 1101 112-113 0.3741447534054494 1101 114-115 0.30304758744536286 1101 116-117 0.25795902706444807 1101 118-119 0.1932777688774472 1101 120-121 0.12373099322109482 1101 122-123 0.3547207190122492 1101 124-125 0.42354979294747963 1102 1 0.3543266862268055 1102 2 0.6319813808581038 1102 3 0.5048789320036136 1102 4 0.6021358402876231 1102 5 0.35858140802852745 1102 6 0.29236266477992956 1102 7 0.31749646460256287 1102 8 0.2807097862218626 1102 9 0.33017115909250805 1102 10-11 0.3146176359087036 1102 12-13 0.34581101452202745 1102 14-15 0.5308944956783819 1102 16-17 0.5706623561015363 1102 18-19 0.40190251678921385 1102 20-21 0.3170322445445066 1102 22-23 0.3531714121004015 1102 24-25 0.36487772415122066 1102 26-27 0.46284360789059775 1102 28-29 0.49168991774759263 1102 30-31 0.5140353330236138 1102 32-33 0.534951930139492 1102 34-35 0.5390273936183974 1102 36-37 0.520388365467781 1102 38-39 0.5273885938253358 1102 40-41 0.5527576686594173 1102 42-43 0.5691848615911539 1102 44-45 0.7153370830063892 1102 46-47 0.7572560129432091 1102 48-49 0.5995902260307702 1102 50-51 0.6178811007859153 1102 52-53 0.5996373382834079 1102 54-55 0.6115639946745262 1102 56-57 0.664598086708601 1102 58-59 0.6320168565731734 1102 60-61 0.5164277063350511 1102 62-63 0.5357447724351587 1102 64-65 0.5458337903993211 1102 66-67 0.5094500341024712 1102 68-69 0.6511417426959767 1102 70-71 0.4832524426005662 1102 72-73 0.3870409318575625 1102 74-75 0.46435183298165583 1102 76-77 0.45331350817260585 1102 78-79 0.4594106927279995 1102 80-81 0.48949597933325606 1102 82-83 0.48652491470235404 1102 84-85 0.4381883835099174 1102 86-87 0.3701037628339492 1102 88-89 0.40116528628831105 1102 90-91 0.4838342155366604 1102 92-93 0.4388529084572781 1102 94-95 0.5251155714881506 1102 96-97 0.5329371755276924 1102 98-99 0.5973799188911393 1102 100-101 0.517438872815358 1102 102-103 0.4589716871970744 1102 104-105 0.5255064343068128 1102 106-107 0.5312230701454439 1102 108-109 0.46547526034864006 1102 110-111 0.5895721265399878 1102 112-113 0.5786147110713245 1102 114-115 0.6928073905291079 1102 116-117 0.6386677075559852 1102 118-119 0.7227164032397297 1102 120-121 0.6563542195236103 1102 122-123 0.5565839901565468 1102 124-125 0.6836613994594956 1103 1 0.22219040254348954 1103 2 0.5288220401987651 1103 3 0.3319343848016416 1103 4 0.4202906021923809 1103 5 0.18217015426579053 1103 6 0.14316757356750998 1103 7 0.21684977218606605 1103 8 0.17487686089636156 1103 9 0.22900697674020876 1103 10-11 0.1625460894683357 1103 12-13 0.10198813978949772 1103 14-15 0.30279850956877397 1103 16-17 0.33231131976164363 1103 18-19 0.22075089201135256 1103 20-21 0.15897828324178676 1103 22-23 0.24939790631575676 1103 24-25 0.27533553160779434 1103 26-27 0.12397791500261945 1103 28-29 0.20918132691598146 1103 30-31 0.22590858765677524 1103 32-33 0.19064533945295636 1103 34-35 0.2806541661857622 1103 36-37 0.2448157944744196 1103 38-39 0.1779906320511344 1103 40-41 0.2569130017751462 1103 42-43 0.2505094363563245 1103 44-45 0.1903595234372375 1103 46-47 0.22391587947931413 1103 48-49 0.33696805035166477 1103 50-51 0.3482158032121987 1103 52-53 0.25213411955494536 1103 54-55 0.2938835418765038 1103 56-57 0.17252001214289692 1103 58-59 0.25483322131404407 1103 60-61 0.30488346468561645 1103 62-63 0.26170529087711714 1103 64-65 0.08306620526168729 1103 66-67 0.2042775147900997 1103 68-69 0.2730514233618564 1103 70-71 0.1897049731558198 1103 72-73 0.23953306637321248 1103 74-75 0.3199892545534837 1103 76-77 0.25917896078929203 1103 78-79 0.16002803124757037 1103 80-81 0.03393630592085728 1103 82-83 0.06934302481006682 1103 84-85 0.06537802555104832 1103 86-87 0.0781138632945435 1103 88-89 0.07591764820032765 1103 90-91 0.030860518441677698 1103 92-93 0.0563916918685905 1103 94-95 -0.017929368308024607 1103 96-97 0.10811896568473145 1103 98-99 0.09117570643692474 1103 100-101 0.09209603289949797 1103 102-103 -0.06633919210513994 1103 104-105 0.07847605132345947 1103 106-107 0.09320252015088215 1103 108-109 0.12128268518453211 1103 110-111 0.12301426196232157 1103 112-113 0.21154411664924666 1103 114-115 0.2740376701504772 1103 116-117 0.22943857035654958 1103 118-119 0.34593046228299684 1103 120-121 0.42373693993850736 1103 122-123 0.3907317524602476 1103 124-125 0.4731113368982953 1104 1 0.22675361609393008 1104 2 0.4941294517652999 1104 3 -0.010494962007264519 1104 4 0.4749895073018706 1104 5 0.18590635115891985 1104 6 0.13292403061411306 1104 7 0.1341984059124357 1104 8 0.13456126104609467 1104 9 0.2111892713312642 1104 10-11 0.16456859555106007 1104 12-13 0.19676342988869777 1104 14-15 0.26536109145160935 1104 16-17 0.2914465200236691 1104 18-19 0.307421535844405 1104 20-21 0.23810342361236536 1104 22-23 0.20458050504306158 1104 24-25 0.16108148425611546 1104 26-27 0.17195306941025734 1104 28-29 0.32002319945949864 1104 30-31 0.17426274262589914 1104 32-33 0.14288132579022061 1104 34-35 0.19771854949102163 1104 36-37 0.321324778191439 1104 38-39 0.2814998965093025 1104 40-41 0.23749975079255137 1104 42-43 0.26889096358821973 1104 44-45 0.3591633321589285 1104 46-47 0.3220185371622577 1104 48-49 0.3537084115719509 1104 50-51 0.4025567633469578 1104 52-53 0.33020238644621003 1104 54-55 0.3271811976878496 1104 56-57 0.4217818968518685 1104 58-59 0.5825803905073883 1104 60-61 0.45109872296747966 1104 62-63 0.3636464989998913 1104 64-65 0.38420262206872735 1104 66-67 0.3246528891116398 1104 68-69 0.42267768669705674 1104 70-71 0.35937615507609166 1104 72-73 0.2611507203129477 1104 74-75 0.28136453529464234 1104 76-77 0.41022062745596344 1104 78-79 0.34073818939842226 1104 80-81 0.3946027152414615 1104 82-83 0.30484157431409287 1104 84-85 0.2715168988432026 1104 86-87 0.33420828590356777 1104 88-89 0.4110401215299184 1104 90-91 0.41336683511769934 1104 92-93 0.28701294865316385 1104 94-95 0.3394979899176924 1104 96-97 0.3601753946572188 1104 98-99 0.3590413717934702 1104 100-101 0.375108268534909 1104 102-103 0.15885089305301037 1104 104-105 0.23921089130099915 1104 106-107 0.3478575594546456 1104 108-109 0.2911620834626518 1104 110-111 0.35215519402295925 1104 112-113 0.3306181387342164 1104 114-115 0.45616615601984023 1104 116-117 0.4117378404295451 1104 118-119 0.41127881526820786 1104 120-121 0.5450112708387493 1104 122-123 0.44297417244901993 1104 124-125 0.564649915412236 1105 1 0.12152125773204858 1105 2 0.43810868199254216 1105 3 0.04694956458144617 1105 4 0.31220988417050677 1105 5 0.05436052333561747 1105 6 -0.07122784140743477 1105 7 0.03426331145232098 1105 8 -0.04860356084547135 1105 9 0.06887675983395525 1105 10-11 0.012644163648076301 1105 12-13 0.1024626222138707 1105 14-15 0.14166640433449373 1105 16-17 0.14981916112380134 1105 18-19 0.07719429703713843 1105 20-21 0.01114471672507733 1105 22-23 0.09291277975662382 1105 24-25 0.06924361621220498 1105 26-27 0.10807595260458669 1105 28-29 0.15056142951667084 1105 30-31 0.166622631696427 1105 32-33 0.06996186072293398 1105 34-35 0.003810645939957169 1105 36-37 0.05906787026358984 1105 38-39 0.013732612372287178 1105 40-41 0.09922526630566608 1105 42-43 0.10468341857952623 1105 44-45 0.11189995103151773 1105 46-47 0.04562159292895984 1105 48-49 0.19891321111101945 1105 50-51 0.2971283000116429 1105 52-53 0.25160351309531137 1105 54-55 0.2932213697048809 1105 56-57 0.2229363130317381 1105 58-59 0.12673108242820774 1105 60-61 0.14437249592303658 1105 62-63 0.31860969026993047 1105 64-65 0.36477399907273167 1105 66-67 0.35254109886412976 1105 68-69 0.3518515120629715 1105 70-71 0.40037528388408816 1105 72-73 0.29316581171868705 1105 74-75 0.33025263744971767 1105 76-77 0.21488063696618553 1105 78-79 0.24129734888903442 1105 80-81 0.23241661335711683 1105 82-83 0.20549444768581182 1105 84-85 0.25898422914298536 1105 86-87 0.20176002811403393 1105 88-89 0.13833203332780286 1105 90-91 0.25148512980273097 1105 92-93 0.2072749310141333 1105 94-95 0.22788510460613054 1105 96-97 0.3175921541700433 1105 98-99 0.2361272865971351 1105 100-101 0.2261031975726766 1105 102-103 0.07280280741934675 1105 104-105 0.2621159521976679 1105 106-107 0.0976671814406167 1105 108-109 0.16170399265737245 1105 110-111 0.14096297991103768 1105 112-113 0.08503848367071853 1105 114-115 0.029733288132113955 1105 116-117 0.0332545475320174 1105 118-119 0.05118600381706884 1105 120-121 0.0623573631434553 1105 122-123 0.044203212645332 1105 124-125 0.021222208729742675 1106 1 0.10289831524077897 1106 2 0.4233069683944173 1106 3 0.31975608251243415 1106 4 0.40239550052879736 1106 5 0.1287701116097395 1106 6 0.03771915184135821 1106 7 -0.005529546738998192 1106 8 0.0024051205295236855 1106 9 0.14975914508941912 1106 10-11 0.15176743882138766 1106 12-13 0.17432435833065796 1106 14-15 0.20223376123946935 1106 16-17 0.14231283049674914 1106 18-19 0.10764393196585331 1106 20-21 0.05697122722016701 1106 22-23 0.12319420590340968 1106 24-25 0.14365408343489605 1106 26-27 0.10310044450638856 1106 28-29 0.13358014321059386 1106 30-31 0.04201035788285168 1106 32-33 0.12294707768699453 1106 34-35 0.08617812466720665 1106 36-37 0.19254258247385536 1106 38-39 0.13518528582619638 1106 40-41 0.16286707542792556 1106 42-43 0.17413200140680374 1106 44-45 0.20471417294669436 1106 46-47 0.28065375805175563 1106 48-49 0.2826571232935109 1106 50-51 0.23924470268611486 1106 52-53 0.21599466981798798 1106 54-55 0.36461660386996186 1106 56-57 0.3327032554245761 1106 58-59 0.38925620937035177 1106 60-61 0.30278220988681426 1106 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2216 70-71 -1.350846427603912 2216 72-73 -1.0438578405593617 2216 74-75 -1.0667872174313189 2216 76-77 -1.0643472737786013 2216 78-79 -1.1136062280116867 2216 80-81 -1.0355585896157038 2216 82-83 -1.0303661394824282 2216 84-85 -1.233805260298908 2216 86-87 -1.2797612554139057 2216 88-89 -1.3731249681339577 2216 90-91 -1.3975784654139751 2216 92-93 -1.492774381255817 2216 94-95 -1.4957421650791538 2216 96-97 -1.4926478909154568 2216 98-99 -1.38540667722879 2216 100-101 -1.630768995590529 2216 102-103 -1.593507401981686 2216 104-105 -1.6546685355711297 2216 106-107 -1.8594417106183485 2216 108-109 -1.787248297721451 2216 110-111 -1.7437283781136514 2216 112-113 -1.768439264167469 2216 114-115 -1.8603806584345186 2216 116-117 -2.019018219766906 2216 118-119 -2.051630079027543 2216 120-121 -2.17711182549359 2216 122-123 -2.3110025780240804 2216 124-125 -2.5027178416392175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24.0 3 8.0 4 43.0 5 168.0 6 465.0 7 814.0 8 1387.0 9 2598.0 10 4574.0 11 6413.0 12 7207.0 13 10138.0 14 15341.0 15 15084.0 16 7770.0 17 6958.0 18 7055.0 19 6587.0 20 6555.0 21 6415.0 22 6803.0 23 7429.0 24 8517.0 25 9711.0 26 11918.0 27 14594.0 28 17904.0 29 22142.0 30 27558.0 31 34724.0 32 45543.0 33 59794.0 34 86019.0 35 141653.0 36 237316.0 37 373522.0 38 157580.0 39 737.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.234862417536114 18.305410099183444 12.52522015974109 22.934507323539354 2 13.533689050556402 22.362853480294945 37.41604671801558 26.687410751133072 3 21.307231744969727 28.350015083330838 28.50559617139046 21.83715700030897 4 12.96327136416891 19.050989432226885 33.59248773618257 34.393251467421635 5 11.713443013787481 38.61459036366345 34.27309673442079 15.398869888128274 6 31.86491388384271 32.08236822902172 18.14118535627232 17.91153253086325 7 24.409306184937492 33.493880508491905 22.569806612965902 19.5270066936047 8 28.09495218645093 32.31183549684895 21.382867761133852 18.21034455556627 9 28.788854899829662 14.786190313410291 20.506286028159302 35.91866875860074 10-11 24.087234559289445 27.390285723676882 25.294881369983585 23.22759834705009 12-13 27.80764346517292 25.128277986953247 26.172179025631493 20.891899522242337 14-15 27.064377829744046 23.3100970344045 26.12266676795341 23.502858367898043 16-17 21.911798702980107 27.347748804564294 30.527445260859352 20.213007231596244 18-19 23.62629175468275 24.661631124239264 32.90150671843911 18.810570402638877 20-21 21.582406698286754 24.53923896319134 33.15993050739318 20.718423831128728 22-23 20.22989362106192 25.666219647234467 29.83266718672849 24.27121954497512 24-25 21.416503782135 26.737861085059322 28.808722430149565 23.036912702656114 26-27 22.756539485255665 23.729135441044566 29.98119888858698 23.533126185112792 28-29 21.881020301804405 22.755083668385478 32.9210326313825 22.442863398427615 30-31 23.61131806455194 24.475373027490235 31.46764806423056 20.445660843727264 32-33 19.461962444524303 24.809761092947905 32.675623124636616 23.05265333789118 34-35 18.384972534204877 25.914701732896102 33.75796573796817 21.94235999493085 36-37 23.235478442268757 24.370557196574605 30.75701864333985 21.63694571781679 38-39 23.969444907046253 23.292488028352135 30.766915887304357 21.97115117729726 40-41 21.128659152546952 25.079524961159684 31.082189549525335 22.709626336768032 42-43 23.93011157955631 24.355839154811886 30.111506513920418 21.602542751711383 44-45 25.171686139768074 22.661730461890865 29.877734341902666 22.288849056438394 46-47 22.024435601445656 24.580590591555715 33.473793850999364 19.921179955999264 48-49 24.102016846497033 24.516459372361343 31.60357995366191 19.777943827479717 50-51 21.10578145132309 25.3934063172903 31.26367718769265 22.23713504369396 52-53 20.28814492779029 26.596012761966538 27.73759228906088 25.378250021182293 54-55 21.523364799995328 27.178087574904975 26.816272091671124 24.48227553342858 56-57 23.109845529951762 24.511492489781368 27.639825048865358 24.738836931401508 58-59 22.414774138318915 23.210790114150647 30.595010620363635 23.779425127166803 60-61 24.10596113560466 25.080200545188408 29.28981613769367 21.524022181513264 62-63 20.07172032360701 25.392201117840752 30.670828622099123 23.86524993645312 64-65 18.747936552457585 26.052139119459373 31.944907046253363 23.25501728182968 66-67 23.327292727607393 25.242865949682557 28.99439618777154 22.435445134938515 68-69 24.119875711067674 24.024263221403174 28.93304057943068 22.922820488098473 70-71 21.612914771216623 25.687840194935536 29.09198082198985 23.607264211857995 72-73 23.960716341335857 25.17559390768026 28.49303321675768 22.370656534226203 74-75 25.154009881174638 23.307717366851026 28.381205316317377 23.15706743565696 76-77 21.941861178553584 24.98626803051419 32.34086984722453 20.731000943707688 78-79 23.636152477451812 24.832002500971466 30.996999418582565 20.534845602994153 80-81 20.673443539692695 25.973910718824776 30.71428884467389 22.63835689680863 82-83 20.01317684731511 26.615295953159375 27.405139846961585 25.966387352563935 84-85 21.137554891356746 26.902389070520965 26.62406104006521 25.33599499805707 86-87 22.742661430988086 24.124148690934273 27.60538556156451 25.52780431651313 88-89 22.075565275063035 23.396573435358945 30.005375919968913 24.522485369609107 90-91 23.357605319823413 25.078812739761645 28.80090689432519 22.762675046089747 92-93 19.84857581946258 25.095795095641705 29.924079738917282 25.13154934597843 94-95 18.536972671365028 25.865768336415133 31.22145620512199 24.37580278709785 96-97 22.910705187787723 24.85934409884353 28.53580904763035 23.694141665738396 98-99 23.456284666918187 23.991379174958848 28.29036008124116 24.261976076881805 100-101 20.989205795475463 25.311416233525293 28.73973389196154 24.95964407903771 102-103 23.453815994433924 24.337083617146 28.412627396124563 23.796472992295513 104-105 24.280001052053258 22.760770395221343 28.451319157889817 24.507909394835586 106-107 21.37723682542744 24.36345744383577 31.95305739503463 22.30624833570216 108-109 23.152793393839637 24.42951147113896 30.198066457575816 22.219628677445584 110-111 20.239891615190093 25.128969960765662 30.471538942298388 24.15959948174586 112-113 19.410842723490862 25.705416978515235 27.66862925798853 27.215111040005375 114-115 20.272924069016543 26.48967400736201 26.737205542514225 26.500196381107223 116-117 21.6987215671918 23.866624430996744 27.5665568027744 26.868097199037056 118-119 21.122482452878298 23.29867623067296 29.54659859447683 26.03224272197191 120-121 22.300481744727527 24.75778529650367 28.406063796019883 24.53566916274892 122-123 19.179080302010444 24.991864651169756 29.27600691148558 26.553048135334222 124-125 18.17796014367706 25.895361173959174 30.175432829879277 25.751245852484494 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2584.0 1 1825.0 2 3081.0 3 3993.0 4 1862.0 5 971.5 6 1817.5 7 4749.5 8 7606.0 9 7631.5 10 5436.0 11 3053.0 12 2128.0 13 2037.5 14 2286.0 15 2606.0 16 2777.5 17 3044.5 18 3324.5 19 3279.0 20 3274.5 21 3538.5 22 3862.0 23 4227.0 24 4630.5 25 5020.5 26 5690.0 27 6709.5 28 7654.0 29 8873.5 30 10640.0 31 12516.0 32 14545.5 33 16842.5 34 18645.5 35 19993.0 36 22606.5 37 25314.0 38 25889.5 39 25225.0 40 24480.0 41 25267.5 42 26313.5 43 27646.0 44 29167.5 45 30758.5 46 32963.0 47 38500.0 48 47200.0 49 73595.0 50 172252.0 51 172486.0 52 68348.5 53 37349.5 54 32693.0 55 29340.5 56 25756.0 57 22248.5 58 20482.5 59 19305.5 60 18126.5 61 16469.0 62 14722.0 63 12212.0 64 9382.5 65 7385.5 66 5278.0 67 4068.0 68 3032.5 69 1954.0 70 1597.0 71 1468.5 72 1383.5 73 1386.5 74 1115.5 75 837.5 76 584.0 77 378.5 78 335.5 79 222.5 80 146.5 81 118.5 82 69.0 83 41.5 84 28.5 85 35.5 86 25.5 87 8.0 88 2.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15382727519743358 2 2.191271726459168E-4 3 5.112967361738059E-4 4 0.0 5 0.0 6 0.0032869075896887518 7 0.0 8 0.0 9 0.0 10-11 3.652119544098613E-5 12-13 0.005003403775415099 14-15 0.0012782418404345146 16-17 4.7477554073281967E-4 18-19 0.0 20-21 1.095635863229584E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.8260597720493065E-4 30-31 0.0 32-33 0.0 34-35 1.095635863229584E-4 36-37 0.0 38-39 0.0 40-41 7.304239088197227E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 6.939027133787364E-4 96-97 0.0025930048763100153 98-99 0.004199937475713405 100-101 0.0 102-103 0.004419064648359321 104-105 0.023081395518703234 106-107 0.018077991743288134 108-109 0.020817081401362094 110-111 0.0176397373979963 112-113 0.01566759284418305 114-115 0.04057504813493559 116-117 0.04064809052581756 118-119 0.023738777036640984 120-121 0.013841533072133743 122-123 0.0031043016124838213 124-125 0.0018625809674902925 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1369068.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.78805090128787 #Duplication Level Percentage of deduplicated Percentage of total 1 80.56802221127055 35.27916657603206 2 11.361089648352273 9.949599436322881 3 3.513886204805192 4.61598683992029 4 1.5342773713279203 2.687320625296045 5 0.8239770635368052 1.8040174799821667 6 0.4747241192229101 1.2472346337961102 7 0.30753929524152235 0.9426582419927384 8 0.2127931944262802 0.7454239383188483 9 0.1600559419486882 0.630768395979246 >10 0.9082840203702366 7.481538220159292 >50 0.07773562313375881 2.3448765056571506 >100 0.047098226632075524 4.112365120817123 >500 0.0034294825212144915 0.9627922039980222 >1k 0.0032008503531335253 3.7406434345619326 >5k 0.002286321680809661 6.582767577153162 >10k+ 0.0016004251765667627 16.872840770012964 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 47778 3.4898193515588707 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 45545 3.3267156927194264 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 24530 1.7917298483347797 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 14431 1.0540747428177417 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 13273 0.9694916541764178 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11537 0.842690063605314 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 11443 0.8358240788624085 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9453 0.6904697210072838 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7954 0.5809791770752074 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 7717 0.56366813043618 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 6695 0.48901880695480426 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 6514 0.4757981342051673 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5790 0.42291544320661945 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 5575 0.4072113291669953 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 5485 0.4006375139876179 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 5464 0.3991036237790965 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 5106 0.37295444784335036 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 4544 0.33190462416768196 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 3872 0.28282013749499657 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 3845 0.28084799294118334 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3805 0.27792629730590446 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 3774 0.2756619831885633 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3092 0.22584707260705825 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2816 0.20568737272363388 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2648 0.19341625105546253 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2513 0.1835555282863963 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 1759 0.1284815655613892 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.4608478176394453E-4 0.0 12-13 0.0 0.0 0.0 4.3825434529183355E-4 0.0 14-15 0.0 0.0 0.0 9.130298860246533E-4 0.0 16-17 0.0 0.0 0.0 0.0018260597720493065 0.0 18-19 0.0 0.0 0.0 0.0029216956352788905 0.0 20-21 0.0 0.0 0.0 0.0048573189936511555 0.0 22-23 0.0 0.0 0.0 0.00803466299701695 0.0 24-25 0.0 0.0 0.0 0.01208851569096641 0.0 26-27 0.0 0.0 0.0 0.01511977491256826 0.0 28-29 0.0 0.0 0.0 0.02048839064239322 0.0 30-31 0.0 0.0 0.0 0.029399562329993835 0.0 32-33 0.0 0.0 0.0 0.043606307356537435 0.0 34-35 0.0 0.0 0.0 0.06340079528555193 0.0 36-37 0.0 0.0 0.0 0.09185080653408012 0.0 38-39 0.0 0.0 0.0 0.13830576713501447 0.0 40-41 0.0 0.0 0.0 0.21876196069150694 0.0 42-43 0.0 0.0 0.0 0.2801175690323636 0.0 44-45 0.0 0.0 0.0 0.3399392871646989 0.0 46-47 0.0 0.0 0.0 0.4173277003041485 0.0 48-49 0.0 0.0 0.0 0.4954830585478589 0.0 50-51 0.0 0.0 0.0 0.5853251993326847 0.0 52-53 0.0 0.0 0.0 0.695071391632848 0.0 54-55 0.0 0.0 0.0 0.8037584692652228 0.0 56-57 0.0 0.0 0.0 0.9331895859080777 0.0 58-59 0.0 0.0 0.0 1.085154280138021 0.0 60-61 0.0 0.0 0.0 1.2256513189994944 0.0 62-63 0.0 0.0 0.0 1.3650892431931796 0.0 64-65 0.0 0.0 0.0 1.5268781389967483 0.0 66-67 0.0 0.0 0.0 1.7019607499408358 0.0 68-69 0.0 0.0 0.0 1.9219279100818951 0.0 70-71 0.0 0.0 0.0 2.2527003771909064 0.0 72-73 0.0 0.0 0.0 2.5193051039101055 0.0 74-75 0.0 0.0 0.0 2.774843908410685 0.0 76-77 0.0 0.0 0.0 3.05945358448229 0.0 78-79 0.0 0.0 0.0 3.3502353425834217 0.0 80-81 0.0 0.0 0.0 3.6707088325780752 0.0 82-83 0.0 0.0 0.0 4.064991658558961 0.0 84-85 0.0 0.0 0.0 4.433855732512921 0.0 86-87 0.0 0.0 0.0 4.800930267890273 0.0 88-89 0.0 0.0 0.0 5.239915037090926 0.0 90-91 0.0 0.0 0.0 5.669952113408538 0.0 92-93 0.0 0.0 0.0 6.116021994524742 0.0 94-95 0.0 0.0 0.0 6.599745228140604 0.0 96-97 0.0 0.0 0.0 7.075725968322976 0.0 98-99 0.0 0.0 0.0 7.614997940204577 0.0 100-101 0.0 0.0 0.0 8.394908068846835 0.0 102-103 0.0 0.0 0.0 9.06090858890866 0.0 104-105 0.0 0.0 0.0 9.669242141369164 0.0 106-107 0.0 0.0 0.0 10.305550929537468 0.0 108-109 0.0 0.0 0.0 10.895222151127628 0.0 110-111 0.0 0.0 0.0 11.538798657188686 0.0 112-113 0.0 0.0 0.0 12.313011479342151 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1165 0.0 50.55927 5 AAAGTAC 1250 0.0 45.217354 3 AAGTACT 1490 0.0 38.333324 4 GTTATCA 825 0.0 35.392906 1 AAAAGTA 1600 0.0 34.582348 2 GTATTAC 155 1.9453182E-7 30.756182 1 AAAAAGT 1835 0.0 30.200977 1 CGTACAC 85 0.0072129085 27.998358 3 TATAACG 90 0.009532311 26.442894 2 GTATACA 270 1.6825652E-9 24.277447 1 TGCACCG 400 0.0 23.798605 5 CTTAACG 75 1.6885137E-4 23.797737 20-21 TTATCAA 1255 0.0 23.703789 2 GAGTACT 11970 0.0 22.918886 12-13 ATAGTAC 350 6.366463E-11 22.098705 3 ATGGGAG 1670 0.0 22.088526 5 TAGTACA 390 1.2732926E-11 21.357723 4 GTATAGG 280 6.56928E-8 21.282179 1 GTACTTT 13175 0.0 20.88816 14-15 TAGGGTC 285 8.052848E-8 20.875969 4 >>END_MODULE