FastQCFastQC Report
Thu 26 May 2016
SRR1364169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586828
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA358252.2576485920339193No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT257721.624120572614045No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG175351.105034698152541No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC95930.6045393703665426No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG86270.5436632073545463No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC81430.5131621070462583No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG74560.4698681898731305No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT64220.4047067483054244No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG57760.36399660202618056No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57310.36116075592313723No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC41960.2644268944082156No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT39680.25005860748612957No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG38550.24293748282737637No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA34600.21804505592288517No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG34490.2173518490976968No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG31540.19876130242219067No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG27760.17494019515662693No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC24640.15527832884219336No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA22950.1446281512552085No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19850.12509232254535463No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC16340.10297272294161686No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT800.005355559329.758236
TACCGTA1004.4321976E-429.7582287
TATCACG900.00951571826.4525952
CATGGGG63700.025.229154
AGTACTC10650.024.03245
ATACGTG1350.00255160322.043137
ATAACGC1400.003149386321.2578953
ATGGGGG27650.020.6659015
ATGGGGA32150.019.9949845
GTATAGG4203.45608E-1119.8394451
CATGGGT23750.019.0470734
ATGGGGC13000.018.7723545
ACCGTCC1600.0068228718.5988948
GTAATAC2902.1842316E-618.4723783
GTATTAG5550.018.2308431
ATGGGAT21700.018.1034985
GCGTTAT3500.017.844772104-105
CATATAG3706.409027E-817.695763
ATGGGAG20600.017.6254775
AGCGTTA3900.017.539734104-105
Produced by FastQC (version 0.11.5)