##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364169_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1586828 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.606806156684907 31.0 31.0 34.0 27.0 34.0 2 30.727686932673233 31.0 31.0 34.0 27.0 34.0 3 30.82568306079802 33.0 31.0 34.0 27.0 34.0 4 34.257169018948495 37.0 35.0 37.0 30.0 37.0 5 34.26661049590756 37.0 35.0 37.0 30.0 37.0 6 34.213914173432784 37.0 35.0 37.0 30.0 37.0 7 34.14294806998616 37.0 35.0 37.0 30.0 37.0 8 34.056781201239204 37.0 35.0 37.0 30.0 37.0 9 35.48193439994757 39.0 37.0 39.0 29.0 39.0 10-11 35.39441294204539 39.0 37.0 39.0 28.0 39.0 12-13 35.28089370744655 39.0 36.0 39.0 27.5 39.0 14-15 36.48523753046959 40.0 37.0 41.0 27.0 41.0 16-17 36.525204369975825 40.0 37.0 41.0 27.0 41.0 18-19 36.51927177992826 40.0 37.0 41.0 27.5 41.0 20-21 36.527013009601546 40.0 37.0 41.0 28.0 41.0 22-23 36.511118407287995 40.0 37.0 41.0 28.0 41.0 24-25 36.5066298930949 40.0 37.0 41.0 28.5 41.0 26-27 36.40516174405796 40.0 36.0 41.0 28.0 41.0 28-29 36.34794886402307 40.0 36.0 41.0 28.0 41.0 30-31 36.43434228536426 40.0 36.0 41.0 29.0 41.0 32-33 36.22802565873555 40.0 36.0 41.0 28.5 41.0 34-35 36.307597609822864 40.0 36.0 41.0 29.0 41.0 36-37 36.30805292066941 40.0 36.0 41.0 29.0 41.0 38-39 36.23536104732208 40.0 36.0 41.0 29.0 41.0 40-41 36.06697543779162 40.0 35.5 41.0 29.0 41.0 42-43 35.9340653177282 40.0 35.0 41.0 28.0 41.0 44-45 35.788875038756565 40.0 35.0 41.0 28.0 41.0 46-47 35.624473162812855 40.0 35.0 41.0 27.0 41.0 48-49 35.46612959942728 39.0 35.0 41.0 27.0 41.0 50-51 35.34881757821263 39.0 35.0 41.0 26.5 41.0 52-53 35.202448217450154 39.0 35.0 41.0 26.0 41.0 54-55 35.00244229368274 39.0 34.0 41.0 26.0 41.0 56-57 34.75022403184214 39.0 34.0 41.0 25.0 41.0 58-59 34.487217266143524 38.0 34.0 40.5 24.5 41.0 60-61 34.22391494226217 38.0 33.0 40.0 23.0 41.0 62-63 33.83386195605321 37.0 33.0 40.0 22.5 41.0 64-65 33.59665697857613 37.0 33.0 40.0 22.0 41.0 66-67 33.260741554850306 36.0 33.0 39.5 22.0 41.0 68-69 32.91280844552781 36.0 32.5 39.0 21.0 41.0 70-71 32.55058928882021 35.5 32.0 39.0 19.0 41.0 72-73 32.35719151666091 35.0 32.0 39.0 19.5 40.5 74-75 32.04176539612359 35.0 32.0 37.5 18.0 40.0 76-77 31.570220591015534 35.0 32.0 37.0 12.0 39.0 78-79 31.190966821860968 35.0 31.5 36.5 8.5 39.0 80-81 30.945927346883217 35.0 31.0 36.0 7.0 38.0 82-83 30.698675596851075 35.0 31.0 36.0 6.0 37.0 84-85 30.450926313374858 35.0 31.0 35.0 2.0 37.0 86-87 30.18013294446531 35.0 31.0 35.0 2.0 36.5 88-89 29.935093154393545 35.0 31.0 35.0 2.0 36.0 90-91 29.768183760306727 35.0 31.0 35.0 2.0 36.0 92-93 29.548872656645838 35.0 30.0 35.0 2.0 36.0 94-95 29.48832702725185 35.0 30.5 35.0 2.0 35.0 96-97 29.42776091674712 35.0 30.5 35.0 2.0 35.0 98-99 29.303986002263635 35.0 30.0 35.0 2.0 35.0 100-101 29.0472644168114 35.0 30.0 35.0 2.0 35.0 102-103 28.01853918635164 33.5 28.0 34.5 2.0 35.0 104-105 28.770985261162522 34.5 29.5 35.0 2.0 35.0 106-107 28.786355862135025 35.0 30.0 35.0 2.0 35.0 108-109 28.740195534739748 35.0 30.0 35.0 2.0 35.0 110-111 28.761596404903365 35.0 30.0 35.0 2.0 35.0 112-113 28.687571998981618 35.0 30.0 35.0 2.0 35.0 114-115 28.594920810573043 35.0 30.0 35.0 2.0 35.0 116-117 28.420643573216505 35.0 29.5 35.0 2.0 35.0 118-119 28.257431807354045 35.0 29.0 35.0 2.0 35.0 120-121 28.09579425117278 35.0 29.0 35.0 2.0 35.0 122-123 27.835715654122566 35.0 29.0 35.0 2.0 35.0 124-125 26.975920515645047 34.0 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2795271062231599 1101 2 0.31261634982488573 1101 3 0.39395709748616525 1101 4 0.3557022863707431 1101 5 0.34573371426534294 1101 6 0.2580568992105583 1101 7 0.24825140849659988 1101 8 0.3022884802791381 1101 9 0.36787362541620894 1101 10-11 0.41978955547941155 1101 12-13 0.40615724933699227 1101 14-15 0.43530647578490544 1101 16-17 0.4439092540839624 1101 18-19 0.40663521639681477 1101 20-21 0.4193845882372287 1101 22-23 0.45178160025238157 1101 24-25 0.5242721005954607 1101 26-27 0.5411846794316659 1101 28-29 0.5950475616578359 1101 30-31 0.425304275791774 1101 32-33 0.42358234997879407 1101 34-35 0.4923053337739205 1101 36-37 0.43715095503058876 1101 38-39 0.3979155107030081 1101 40-41 0.432415154331359 1101 42-43 0.3831307904175816 1101 44-45 0.4240733182704375 1101 46-47 0.42426092493777645 1101 48-49 0.3925652392458687 1101 50-51 0.31040520467180244 1101 52-53 0.3133823820327919 1101 54-55 0.2931289380396862 1101 56-57 0.29304718530178064 1101 58-59 0.3803524869441972 1101 60-61 0.35579199031730013 1101 62-63 0.3131111611681803 1101 64-65 0.33531589363169445 1101 66-67 0.2647201734712752 1101 68-69 0.20140899613943475 1101 70-71 0.222532701438503 1101 72-73 0.17981898920420036 1101 74-75 0.2602195875804654 1101 76-77 0.3043436407678364 1101 78-79 0.18800268866257852 1101 80-81 0.20092284908209024 1101 82-83 0.1787529627227613 1101 84-85 0.23081133517565533 1101 86-87 0.2978257082275242 1101 88-89 0.32004214753320426 1101 90-91 0.331012196873683 1101 92-93 0.2390788780048645 1101 94-95 0.29343499476142654 1101 96-97 0.2784981201627623 1101 98-99 0.30984027593125774 1101 100-101 0.26927570979127324 1101 102-103 0.21151528516213958 1101 104-105 0.4305136128732059 1101 106-107 0.5042500092297892 1101 108-109 0.36445562654910546 1101 110-111 0.3496860827345891 1101 112-113 0.4271714056492826 1101 114-115 0.3627302383999904 1101 116-117 0.3328060290232777 1101 118-119 0.36736028446608415 1101 120-121 0.35179755474201 1101 122-123 0.2984661821661021 1101 124-125 0.3311223096259681 1102 1 0.14995297910173022 1102 2 0.22843755914042063 1102 3 0.299031145640285 1102 4 0.4255861826902816 1102 5 0.2286451158409264 1102 6 0.2633422754789265 1102 7 0.3635150651322405 1102 8 0.342643311557147 1102 9 0.4581995503117753 1102 10-11 0.2639718381128304 1102 12-13 0.23131782827604752 1102 14-15 0.38389839445871843 1102 16-17 0.3082117191680922 1102 18-19 0.3899979270185625 1102 20-21 0.36073922535707226 1102 22-23 0.4096358773102722 1102 24-25 0.4742309144340098 1102 26-27 0.4934387976855703 1102 28-29 0.5889527704575457 1102 30-31 0.5072600280169866 1102 32-33 0.5023906390831812 1102 34-35 0.47313946040270594 1102 36-37 0.33382096150735663 1102 38-39 0.4262654996186086 1102 40-41 0.39197320544712255 1102 42-43 0.3824811212833694 1102 44-45 0.4569367598831633 1102 46-47 0.4841531019578511 1102 48-49 0.5132932164221415 1102 50-51 0.5966606182793797 1102 52-53 0.5371715740885321 1102 54-55 0.6541761749807335 1102 56-57 0.6192144163311539 1102 58-59 0.606061707573069 1102 60-61 0.6210603922362878 1102 62-63 0.5165584827274188 1102 64-65 0.45149075333793576 1102 66-67 0.5035897672895118 1102 68-69 0.5188840272554529 1102 70-71 0.42060698577007827 1102 72-73 0.3698168737496417 1102 74-75 0.3820654231053169 1102 76-77 0.34677082059161535 1102 78-79 0.27063853779299407 1102 80-81 0.2964041077485682 1102 82-83 0.3070042062767797 1102 84-85 0.38107779551305754 1102 86-87 0.29018670764939714 1102 88-89 0.3321024119070657 1102 90-91 0.3109148352512143 1102 92-93 0.3350145609416977 1102 94-95 0.28223811838946133 1102 96-97 0.3073628455376429 1102 98-99 0.3767500387540288 1102 100-101 0.25580761765598226 1102 102-103 0.29729660511884504 1102 104-105 0.3317093880496458 1102 106-107 0.2972042415153915 1102 108-109 0.2822363480929795 1102 110-111 0.35700830848256615 1102 112-113 0.41843935984534397 1102 114-115 0.46399675814398833 1102 116-117 0.34161975890291174 1102 118-119 0.29469242821846464 1102 120-121 0.22364320914918423 1102 122-123 0.28579681901934606 1102 124-125 0.2941110919210601 1103 1 0.12466713917208949 1103 2 0.1663916325903827 1103 3 0.0905756790209189 1103 4 0.05410883185549409 1103 5 0.07870202514818203 1103 6 0.05430515139446612 1103 7 0.09769279955055055 1103 8 0.0357731756399815 1103 9 0.1447186962287219 1103 10-11 0.12236836294967901 1103 12-13 0.11933232167235275 1103 14-15 0.08457446963813453 1103 16-17 0.09455681129465177 1103 18-19 0.09573541990490497 1103 20-21 0.010279331637057965 1103 22-23 0.0845239064005412 1103 24-25 0.1116258724171999 1103 26-27 0.2373689027091146 1103 28-29 0.2647238176627056 1103 30-31 0.021524493697711478 1103 32-33 0.14710380139296575 1103 34-35 -0.03351042910454538 1103 36-37 0.10406496940074561 1103 38-39 0.0644342763641319 1103 40-41 0.04750486402083709 1103 42-43 0.027311428076266964 1103 44-45 0.004789542157027427 1103 46-47 -0.04423096934877435 1103 48-49 -0.02378393633870246 1103 50-51 0.004518352402058667 1103 52-53 0.06378628329675706 1103 54-55 0.17364303824319194 1103 56-57 0.17751134988029804 1103 58-59 0.1807470434488394 1103 60-61 0.10090433509046903 1103 62-63 0.051263736860732934 1103 64-65 0.10900278189605928 1103 66-67 0.20094219110864486 1103 68-69 0.08804010657371464 1103 70-71 0.07140371607169982 1103 72-73 0.03570584147394129 1103 74-75 0.046137727488812175 1103 76-77 0.01599378682296404 1103 78-79 0.11335155967721278 1103 80-81 0.08348033665702204 1103 82-83 0.07415534113705746 1103 84-85 0.13111659731345782 1103 86-87 0.11186638215772149 1103 88-89 0.13141506572848627 1103 90-91 0.04748423873820329 1103 92-93 -0.056005778592535194 1103 94-95 0.016467403771159184 1103 96-97 0.016713340352982442 1103 98-99 0.058828965311459314 1103 100-101 0.017652144384875612 1103 102-103 0.07555872790991458 1103 104-105 0.11684811372461468 1103 106-107 -0.016424059409199998 1103 108-109 -0.00436298821146508 1103 110-111 0.08402045898920818 1103 112-113 0.10618722944172987 1103 114-115 0.1477336302015182 1103 116-117 0.1317857197463539 1103 118-119 0.1568155888283549 1103 120-121 -0.012358025117610083 1103 122-123 -0.043389340932762366 1103 124-125 0.07538497350535422 1104 1 0.05658341021222668 1104 2 0.0764934376628652 1104 3 0.11572102416804242 1104 4 0.13552750204259922 1104 5 0.055034249880975494 1104 6 0.11405660484593483 1104 7 0.05899290871808205 1104 8 0.07609788558438169 1104 9 0.2452467624400967 1104 10-11 0.21765829160996475 1104 12-13 0.20178998751814703 1104 14-15 0.3053981767644842 1104 16-17 0.19651294117863216 1104 18-19 0.15603271102678917 1104 20-21 0.2530647539630806 1104 22-23 0.1825430234128973 1104 24-25 0.21247001672144705 1104 26-27 0.2767663233087774 1104 28-29 0.29450243605871407 1104 30-31 0.2178337424727559 1104 32-33 0.2268246625470951 1104 34-35 0.3203066264185708 1104 36-37 0.35737236486648527 1104 38-39 0.25761130548394107 1104 40-41 0.30111120081537734 1104 42-43 0.3402560318996706 1104 44-45 0.245538863033417 1104 46-47 0.21237874402297763 1104 48-49 0.22093413215758062 1104 50-51 0.2303206068385748 1104 52-53 0.24203655621559506 1104 54-55 0.2849171249171363 1104 56-57 0.30642424434321924 1104 58-59 0.3593324353095113 1104 60-61 0.3587751873588729 1104 62-63 0.41742751354965435 1104 64-65 0.5289220486964368 1104 66-67 0.3973326808392841 1104 68-69 0.4476119413499191 1104 70-71 0.3404001363920983 1104 72-73 0.24425835613538993 1104 74-75 0.20568925202611865 1104 76-77 0.25261759042295395 1104 78-79 0.24412918794906346 1104 80-81 0.271259285094402 1104 82-83 0.33653062357631924 1104 84-85 0.21613398795551575 1104 86-87 0.2340153323722518 1104 88-89 0.3127994623874564 1104 90-91 0.30131398816062926 1104 92-93 0.236053552692848 1104 94-95 0.3936786810313109 1104 96-97 0.35654171883428276 1104 98-99 0.3080044965511455 1104 100-101 0.22847750171814596 1104 102-103 0.14204683372625837 1104 104-105 0.15754792839368292 1104 106-107 0.16222480194803168 1104 108-109 0.18233253591963816 1104 110-111 0.22028883340846406 1104 112-113 0.17115968368022294 1104 114-115 0.1143055157533226 1104 116-117 0.1596875451290778 1104 118-119 0.02235419097616287 1104 120-121 -0.03702798373550209 1104 122-123 -0.038575179618689504 1104 124-125 0.22112743967611337 1105 1 0.1658400039044139 1105 2 0.325619361278914 1105 3 0.3063660654656495 1105 4 0.30831480521337085 1105 5 0.2840014313839063 1105 6 0.22244913150387902 1105 7 0.2814405201344883 1105 8 0.27000253554904674 1105 9 0.27854711930207543 1105 10-11 0.35636909442330733 1105 12-13 0.36385820313241624 1105 14-15 0.3816830024668789 1105 16-17 0.45325928697229756 1105 18-19 0.4290980007361682 1105 20-21 0.37449747905131403 1105 22-23 0.36789395650476564 1105 24-25 0.4072100725868566 1105 26-27 0.6315508541983377 1105 28-29 0.7320157855328162 1105 30-31 0.5724948982060667 1105 32-33 0.3752141096764703 1105 34-35 0.5141040396498582 1105 36-37 0.5500455561593611 1105 38-39 0.5984077922110131 1105 40-41 0.6128158433369606 1105 42-43 0.5714068390236449 1105 44-45 0.543160570993571 1105 46-47 0.5953352290145162 1105 48-49 0.6532271868682429 1105 50-51 0.5428301421772304 1105 52-53 0.6006834614569456 1105 54-55 0.5052309369907562 1105 56-57 0.613154548720928 1105 58-59 0.5017269433777543 1105 60-61 0.47098423055498984 1105 62-63 0.4480573582733314 1105 64-65 0.5859661704390504 1105 66-67 0.5280744539034217 1105 68-69 0.5460575554821787 1105 70-71 0.5399967110956183 1105 72-73 0.4701770512901078 1105 74-75 0.4989071106814009 1105 76-77 0.49203673771807743 1105 78-79 0.4160559516097102 1105 80-81 0.4284825345120531 1105 82-83 0.5438761105236836 1105 84-85 0.5819146934215382 1105 86-87 0.5927708853566358 1105 88-89 0.5240991275019518 1105 90-91 0.45432334544749864 1105 92-93 0.36992556247679076 1105 94-95 0.5088558712137186 1105 96-97 0.4328359806637039 1105 98-99 0.331250263213537 1105 100-101 0.3074510063080709 1105 102-103 0.26144849861181996 1105 104-105 0.3201570016443114 1105 106-107 0.2840814472478961 1105 108-109 0.38196412130009705 1105 110-111 0.31480408016141936 1105 112-113 0.32835912231003306 1105 114-115 0.36570281215301037 1105 116-117 0.2848403089706828 1105 118-119 0.3371475336209855 1105 120-121 0.4021322785641388 1105 122-123 0.4548115945292892 1105 124-125 0.5126621401954878 1106 1 0.30513490149825984 1106 2 0.2689671760821142 1106 3 0.30653586840640656 1106 4 0.2973849033460354 1106 5 0.17628884748773999 1106 6 0.24138405375187233 1106 7 0.33781699081997374 1106 8 0.3171531612625458 1106 9 0.3965295090494223 1106 10-11 0.28174406013121 1106 12-13 0.29265040996961744 1106 14-15 0.3429276339126872 1106 16-17 0.34315129333526073 1106 18-19 0.3763791818344515 1106 20-21 0.33029308935253 1106 22-23 0.33519629925148564 1106 24-25 0.4839232869582375 1106 26-27 0.5981538361515177 1106 28-29 0.5674815579269605 1106 30-31 0.5090390570451788 1106 32-33 0.3305431063184727 1106 34-35 0.49033627771977706 1106 36-37 0.46272551326431 1106 38-39 0.3580425161202143 1106 40-41 0.39369608387161037 1106 42-43 0.4063718132923029 1106 44-45 0.29314072333745855 1106 46-47 0.3562028324905455 1106 48-49 0.25359639632533515 1106 50-51 0.33416127151660646 1106 52-53 0.25430957225836437 1106 54-55 0.21298948478641222 1106 56-57 0.32000285975858134 1106 58-59 0.31048353981729804 1106 60-61 0.38261462982783456 1106 62-63 0.4144655662650578 1106 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-1.2591667416841013 2216 90-91 -1.3162439998539917 2216 92-93 -1.4379852463908662 2216 94-95 -1.3688796345235303 2216 96-97 -1.4210388530353875 2216 98-99 -1.4370470102289374 2216 100-101 -1.5112265436542174 2216 102-103 -1.4652152428718317 2216 104-105 -1.5276349297931517 2216 106-107 -1.526869871694867 2216 108-109 -1.4193441588935904 2216 110-111 -1.557751936793295 2216 112-113 -1.6319577041424367 2216 114-115 -1.6607000195899957 2216 116-117 -1.7362805816640794 2216 118-119 -1.7001778453094154 2216 120-121 -1.6730603637222572 2216 122-123 -1.6371022165515683 2216 124-125 -1.918087697902017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41450.0 3 7314.0 4 4911.0 5 3445.0 6 3502.0 7 3415.0 8 3870.0 9 4500.0 10 5452.0 11 6459.0 12 7068.0 13 7846.0 14 8109.0 15 8028.0 16 7783.0 17 7646.0 18 7753.0 19 7969.0 20 8448.0 21 9228.0 22 10579.0 23 12765.0 24 15401.0 25 17248.0 26 19900.0 27 21673.0 28 25328.0 29 30996.0 30 36633.0 31 42775.0 32 53866.0 33 69256.0 34 90298.0 35 125827.0 36 217357.0 37 419436.0 38 211955.0 39 1339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.30385514521697 17.985970928745285 11.091615736671875 23.618558189365867 2 15.171458853294128 22.225641142527188 37.65662137230396 24.946278631874723 3 20.602962587833133 28.23536114452111 28.425777046274607 22.73589922137115 4 13.264332016610958 17.54640180289679 34.89668793679733 34.292578243694926 5 12.525884307984489 40.04171876236449 33.46971591358708 13.962681016063938 6 31.059634807790076 33.230515790786555 19.654231695280206 16.055617706143156 7 26.78688422893033 33.19139077333269 21.90489996182933 18.11682503590765 8 27.408907344018907 33.84741339026902 21.53792187300152 17.20575739271056 9 29.12646320342208 16.047823044602968 19.663490917611 35.16222283436395 10-11 24.348174114921726 28.414393539037043 26.42510671538269 20.812325630658542 12-13 25.748104447432098 26.19408887771509 26.044984107945645 22.012822566907165 14-15 24.9344156064107 26.33087963653251 24.220814351293026 24.513890405763767 16-17 22.849734174738717 29.350217579733336 27.137413014009564 20.662635231518387 18-19 23.534956655728383 28.420054057287807 27.800000063306346 20.24498922367746 20-21 22.910167323367435 28.37235392641849 27.44486894949719 21.27260980071688 22-23 22.182483646886734 29.265202221363847 24.88639889565036 23.66591523609906 24-25 22.19745925639135 29.929318968139878 24.57187593289813 23.30134584257064 26-27 23.135331085466433 27.82647100726599 25.12250874610185 23.915689161165723 28-29 22.898533472927202 27.8838327444283 26.546251654564063 22.671382128080435 30-31 24.219187589704653 28.71052322450092 25.777851939076985 21.292437246717437 32-33 21.367268610016826 28.950621310149742 27.074944131796403 22.607165948037032 34-35 20.590669447747718 29.59875687196769 27.180128188080104 22.630445492204487 36-37 23.492037270693555 28.307745571154108 26.001096867030032 22.199120291122306 38-39 24.516633746303576 27.578259147579814 25.711858037102786 22.193249069013817 40-41 22.407374018226275 29.282368087253314 25.799605867076615 22.510652027443797 42-43 23.814842934421563 28.536522096738477 25.411407421467207 22.23722754737275 44-45 24.44523557492243 27.737794043420404 25.46826221861467 22.348708163042495 46-47 22.562440482199804 28.34928755867913 27.833458711696963 21.2548132474241 48-49 23.61231896441506 27.98495890345064 27.352339142422043 21.050382989712254 50-51 21.66781634654938 28.674113217099645 27.548334018922255 22.109736417428724 52-53 21.43694272352759 29.36417483530878 25.226675863640104 23.972206577523526 54-55 21.758589812145814 29.690928133197065 24.745714981822093 23.804767072835027 56-57 22.771740368335404 27.935845002518167 25.171716072472535 24.120698556673897 58-59 22.80447329283212 27.673192354185982 26.3951674416059 23.127166911375994 60-61 23.79753988425553 28.590168332867698 25.92866569237384 21.683626090502923 62-63 21.17586346877129 28.91714287793498 26.940959716655744 22.966033936637984 64-65 20.22219746891843 29.31895222640497 27.203240938814055 23.25560936586255 66-67 22.811109339165565 28.547371698485748 25.883694557089555 22.757824405259132 68-69 23.8084238644623 27.51645318164458 25.792076842583896 22.883046111309227 70-71 21.891463899360243 29.00820394285468 25.896724752084214 23.20360740570086 72-73 23.321705618929748 28.32873256436198 25.595170094484804 22.75439172222347 74-75 23.91080617495712 27.544036761122324 25.555151177583816 22.990005886336736 76-77 22.236739844766568 28.109510199535208 27.806074659888917 21.847675295809303 78-79 23.099544733681228 28.045076048587052 27.239943196129033 21.615436021602687 80-81 21.509366971161924 28.531628657764184 27.344923076630973 22.61408129444292 82-83 21.215197371793614 29.1365549662076 25.32224450648033 24.326003155518457 84-85 21.576087162889955 29.466553848373735 24.823589570931865 24.133769417804448 86-87 22.460964756969002 27.491393275188297 25.439576662112913 24.60806530572979 88-89 22.498909976389676 27.229485882216697 26.591545213673108 23.68005892772052 90-91 23.334068491187125 28.288473410437366 26.009747750148005 22.367710348227504 92-93 21.216432599381026 28.30193097599413 26.8671797370936 23.61445668753125 94-95 20.372210446904425 28.551492455604166 27.30254226614778 23.773754831343624 96-97 22.803328484776394 27.755626403397827 26.273537065520053 23.16750804630572 98-99 23.604498697336037 27.030198147870543 25.82838864990376 23.536914504889655 100-101 21.781322767938608 28.15420896951665 26.086322639855965 23.97814562268878 102-103 23.005657963550497 27.587678831045885 25.943283768973973 23.463379436429648 104-105 23.614114581768796 26.807808268340843 26.023629703292762 23.5544474465976 106-107 22.024324584174597 27.510417773594643 27.84581695079926 22.619440691431492 108-109 22.614673458382292 27.587037965395144 27.29273380259639 22.505554773626173 110-111 21.266377300224377 28.157489072836896 27.446863634508734 23.12926999242999 112-113 20.933207478414026 28.655033100181193 25.600383638185665 24.81137578321912 114-115 21.11012277196654 29.377302053453825 25.130230904423108 24.38234427015653 116-117 22.03828113734052 27.655822684574964 25.516446860777236 24.78944931730728 118-119 22.01457973679255 27.4619960029938 26.36277182727743 24.160652432936217 120-121 22.572496047828004 28.40094883776868 26.03483608178741 22.991719032615904 122-123 21.063298811425224 28.538036891908437 26.45818129363678 23.940483003029563 124-125 20.44672871003629 28.92795042097695 26.77740462342357 23.84791624556319 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7137.0 1 4227.5 2 1856.5 3 2447.5 4 2267.0 5 1777.0 6 1375.0 7 1199.0 8 1164.0 9 1103.5 10 975.5 11 897.0 12 900.0 13 946.0 14 991.0 15 1020.0 16 1115.5 17 1248.0 18 1413.5 19 1645.5 20 1970.5 21 2495.5 22 3203.5 23 4091.0 24 5166.0 25 6416.0 26 8312.5 27 10438.5 28 11908.5 29 13648.0 30 16568.0 31 19248.0 32 21567.5 33 24438.5 34 27937.5 35 30463.5 36 32868.0 37 35480.5 38 36611.5 39 37060.0 40 37385.0 41 39707.5 42 42168.5 43 44210.0 44 46453.5 45 48294.5 46 50290.5 47 53793.5 48 60612.5 49 76802.5 50 125436.0 51 130654.0 52 78936.5 53 53776.5 54 45959.0 55 40490.0 56 35548.0 57 31221.0 58 28412.0 59 25607.5 60 22090.0 61 18650.0 62 15550.0 63 12255.5 64 9541.0 65 7541.5 66 5424.0 67 4315.5 68 3345.5 69 2191.5 70 1696.0 71 1436.5 72 1302.0 73 1133.0 74 817.5 75 636.0 76 436.0 77 214.0 78 134.0 79 90.0 80 51.5 81 32.5 82 22.0 83 14.0 84 19.0 85 17.5 86 10.0 87 8.0 88 9.5 89 11.5 90 8.0 91 5.5 92 7.0 93 7.5 94 4.0 95 2.0 96 2.0 97 1.5 98 1.0 99 1.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47490969405631867 2 0.46520479850368157 3 0.44919802272205933 4 0.4504583987678563 5 0.45392443289379825 6 0.4619908395868991 7 0.4462361390144364 8 0.4727670547784637 9 0.4835432699700283 10-11 0.48804911433375264 12-13 0.4875764733165787 14-15 0.4647321574865077 16-17 0.4667802685609278 18-19 0.45420801750410256 20-21 0.4626525370109426 22-23 0.48007723584408646 24-25 0.4668747967643626 26-27 0.47598101369524615 28-29 0.4716642257383913 30-31 0.48445704260323114 32-33 0.4830391195517095 34-35 0.4780606341708112 36-37 0.4331597375392922 38-39 0.3936154391024106 40-41 0.38712450246655594 42-43 0.3956320407756858 44-45 0.4291895529950316 46-47 0.4375710536995818 48-49 0.4341995477770748 50-51 0.4208395616916263 52-53 0.41579805750843823 54-55 0.416743339542786 56-57 0.3977746800535408 58-59 0.40215448681268545 60-61 0.4119224011676124 62-63 0.41337183362027896 64-65 0.42802370515266935 66-67 0.41869692241377143 68-69 0.4198627702561336 70-71 0.4320569084992198 72-73 0.4309540794591474 74-75 0.4347036981953936 76-77 0.42575502827023476 78-79 0.41932711043666987 80-81 0.427362007728626 82-83 0.4246837086313072 84-85 0.42260408815574213 86-87 0.42937860940190115 88-89 0.41510485068324987 90-91 0.42024088306987273 92-93 0.42840181796640847 94-95 0.427330498327481 96-97 0.3963252476008742 98-99 0.3678407489658615 100-101 0.36661188232120934 102-103 0.3476747322331091 104-105 0.35010095612126835 106-107 0.37029848225516565 108-109 0.3768839470944551 110-111 0.3937729861081352 112-113 0.3889520477329616 114-115 0.38907808533754135 116-117 0.3614758499345865 118-119 0.43454615118966894 120-121 0.44116312543010333 122-123 0.39307977928294685 124-125 0.3881643127043385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1586828.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.48818122458949 #Duplication Level Percentage of deduplicated Percentage of total 1 87.05424247852824 55.269155228461564 2 8.890707949459301 11.289097550203408 3 1.9862613693834748 3.78312365336458 4 0.7183250260549715 1.8242059772934398 5 0.34193769579655825 1.085450119912522 6 0.21226872303534605 0.8085933093828147 7 0.13914934841739618 0.6184037334725034 8 0.10319862049303286 0.5241514175991444 9 0.07414781113509797 0.4236758703676561 >10 0.4216558404617073 5.01678095992608 >50 0.039485357032906857 1.733229320739465 >100 0.014746866267122396 1.694710102832976 >500 4.468746847866847E-4 0.1900874485152029 >1k 0.001936456967408967 3.3618998543616656 >5k 0.0010427075978355978 4.8938533760023475 >10k+ 4.468746847866847E-4 7.4835820775646935 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 35825 2.2576485920339193 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 25772 1.624120572614045 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 17535 1.105034698152541 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 9593 0.6045393703665426 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 8627 0.5436632073545463 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 8143 0.5131621070462583 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 7456 0.4698681898731305 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 6422 0.4047067483054244 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 5776 0.36399660202618056 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5731 0.36116075592313723 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 4196 0.2644268944082156 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 3968 0.25005860748612957 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 3855 0.24293748282737637 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 3460 0.21804505592288517 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 3449 0.2173518490976968 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 3154 0.19876130242219067 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 2776 0.17494019515662693 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 2464 0.15527832884219336 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 2295 0.1446281512552085 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1985 0.12509232254535463 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 1634 0.10297272294161686 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.205658080144792E-4 0.0 14-15 0.0 0.0 0.0 6.932068251883632E-4 0.0 16-17 0.0 0.0 0.0 0.0013864136503767264 0.0 18-19 0.0 0.0 0.0 0.001890564068695536 0.0 20-21 0.0 0.0 0.0 0.002457733289304197 0.0 22-23 0.0 0.0 0.0 0.003970184544260625 0.0 24-25 0.0 0.0 0.0 0.005955276816390938 0.0 26-27 0.0 0.0 0.0 0.007656784478216921 0.0 28-29 0.0 0.0 0.0 0.012477722853390537 0.0 30-31 0.0 0.0 0.0 0.023222428643810168 0.0 32-33 0.0 0.0 0.0 0.04546806585212764 0.0 34-35 3.15094011449256E-5 6.30188022898512E-5 0.0 0.08031746351841536 0.0 36-37 6.30188022898512E-5 6.30188022898512E-5 0.0 0.13558495312661484 0.0 38-39 6.30188022898512E-5 6.30188022898512E-5 0.0 0.21549279443014618 0.0 40-41 6.30188022898512E-5 6.30188022898512E-5 0.0 0.31317193797941556 0.0 42-43 9.45282034347768E-5 6.30188022898512E-5 0.0 0.39834184927414945 0.0 44-45 1.260376045797024E-4 6.30188022898512E-5 0.0 0.5006843841928678 0.0 46-47 1.260376045797024E-4 6.30188022898512E-5 0.0 0.6253670845233383 0.0 48-49 1.260376045797024E-4 6.30188022898512E-5 0.0 0.7456384686935194 0.0 50-51 1.260376045797024E-4 6.30188022898512E-5 0.0 0.8678319263335409 3.15094011449256E-5 52-53 1.260376045797024E-4 6.30188022898512E-5 0.0 1.0107585699269235 6.30188022898512E-5 54-55 1.260376045797024E-4 6.30188022898512E-5 0.0 1.1706687807374208 6.30188022898512E-5 56-57 1.890564068695536E-4 6.30188022898512E-5 0.0 1.3540850048020328 6.30188022898512E-5 58-59 1.890564068695536E-4 6.30188022898512E-5 0.0 1.56069214810931 6.30188022898512E-5 60-61 3.1509401144925596E-4 6.30188022898512E-5 0.0 1.7598315633452397 6.30188022898512E-5 62-63 3.781128137391072E-4 6.30188022898512E-5 0.0 1.9574270179250681 6.30188022898512E-5 64-65 3.781128137391072E-4 6.30188022898512E-5 0.0 2.183948102755938 6.30188022898512E-5 66-67 3.781128137391072E-4 6.30188022898512E-5 0.0 2.445822735671415 6.30188022898512E-5 68-69 3.781128137391072E-4 6.30188022898512E-5 0.0 2.7458237439722515 6.30188022898512E-5 70-71 3.781128137391072E-4 6.30188022898512E-5 0.0 3.1373910720002423 6.30188022898512E-5 72-73 3.781128137391072E-4 6.30188022898512E-5 0.0 3.47488826766354 6.30188022898512E-5 74-75 3.781128137391072E-4 6.30188022898512E-5 0.0 3.8104318804558526 6.30188022898512E-5 76-77 3.781128137391072E-4 6.30188022898512E-5 0.0 4.205937883626959 6.30188022898512E-5 78-79 3.781128137391072E-4 6.30188022898512E-5 0.0 4.612755761809094 6.30188022898512E-5 80-81 4.411316160289584E-4 6.30188022898512E-5 0.0 5.037502489242691 6.30188022898512E-5 82-83 4.411316160289584E-4 6.30188022898512E-5 0.0 5.519942930172646 6.30188022898512E-5 84-85 4.411316160289584E-4 6.30188022898512E-5 0.0 6.002540918108327 6.30188022898512E-5 86-87 4.411316160289584E-4 6.30188022898512E-5 0.0 6.52001981311144 6.30188022898512E-5 88-89 4.411316160289584E-4 6.30188022898512E-5 0.0 7.093270348141071 6.30188022898512E-5 90-91 4.411316160289584E-4 6.30188022898512E-5 0.0 7.663023339643616 6.30188022898512E-5 92-93 4.411316160289584E-4 6.30188022898512E-5 0.0 8.240685190833538 6.30188022898512E-5 94-95 4.411316160289584E-4 6.30188022898512E-5 0.0 8.852125120050818 6.30188022898512E-5 96-97 4.411316160289584E-4 6.30188022898512E-5 0.0 9.488930117189765 6.30188022898512E-5 98-99 4.411316160289584E-4 6.30188022898512E-5 0.0 10.19187334733191 6.30188022898512E-5 100-101 4.411316160289584E-4 6.30188022898512E-5 0.0 11.061343762524988 6.30188022898512E-5 102-103 4.411316160289584E-4 6.30188022898512E-5 0.0 11.861556514001517 6.30188022898512E-5 104-105 5.041504183188096E-4 6.30188022898512E-5 0.0 12.62480873793505 6.30188022898512E-5 106-107 5.671692206086608E-4 6.30188022898512E-5 0.0 13.414245274220017 6.30188022898512E-5 108-109 5.671692206086608E-4 6.30188022898512E-5 0.0 14.188242203943968 6.30188022898512E-5 110-111 5.671692206086608E-4 6.30188022898512E-5 0.0 15.01552153100399 6.30188022898512E-5 112-113 5.671692206086608E-4 6.30188022898512E-5 0.0 15.920250966078239 6.30188022898512E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 80 0.0053555593 29.75823 6 TACCGTA 100 4.4321976E-4 29.758228 7 TATCACG 90 0.009515718 26.452595 2 CATGGGG 6370 0.0 25.22915 4 AGTACTC 1065 0.0 24.0324 5 ATACGTG 135 0.002551603 22.04313 7 ATAACGC 140 0.0031493863 21.257895 3 ATGGGGG 2765 0.0 20.665901 5 ATGGGGA 3215 0.0 19.994984 5 GTATAGG 420 3.45608E-11 19.839445 1 CATGGGT 2375 0.0 19.047073 4 ATGGGGC 1300 0.0 18.772354 5 ACCGTCC 160 0.00682287 18.598894 8 GTAATAC 290 2.1842316E-6 18.472378 3 GTATTAG 555 0.0 18.230843 1 ATGGGAT 2170 0.0 18.103498 5 GCGTTAT 350 0.0 17.844772 104-105 CATATAG 370 6.409027E-8 17.69576 3 ATGGGAG 2060 0.0 17.625477 5 AGCGTTA 390 0.0 17.539734 104-105 >>END_MODULE