FastQCFastQC Report
Thu 26 May 2016
SRR1364168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345680
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA785925.840318649307413No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT617354.587643421913085No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG386672.873417157125022No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC176691.3130164675108495No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG176451.3112329825812972No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG152981.1368230188454909No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT131020.9736341477914511No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT86960.6462160394744665No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC85400.6346233874323761No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG77800.5781463646632186No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG75600.5617977528089888No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA73580.5467867546519232No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG64720.4809464360026158No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG59080.4390345401581357No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC55920.4155519885856965No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA50630.37624100826348017No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49830.37029605849830566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43280.3216217822959396No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC35960.26722549194459305No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA27210.20220260388799713No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG25150.18689435824267284No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC21490.15969621306699958No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA21100.156798050056477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTC14450.048.203985
AAGTACT17150.040.2633674
GAACAAA15400.035.5564541
AAAGTAC20350.033.0544743
AAAAGTA21300.031.5778522
AAAAAGT21500.031.2817731
ACCGACT850.007195969628.0118038
GTTAGAC1107.731157E-427.0578653
GTACAAA13250.026.5025391
GATATAG2809.458745E-1125.5078891
TGGGATA9300.024.9621326
ATATAGG2901.4551915E-1024.6301482
GGGATAG3252.3646862E-1123.8100347
TCGCGCT1001.828379E-623.79847560-61
GTACATA8350.023.5222451
ATGGGAT29550.023.3703615
ATGGGAG23750.023.0615835
ACCTATC1300.002047313622.8942627
CGCAAAA2352.898596E-722.7959882
GTCGGGA3950.022.6036172
Produced by FastQC (version 0.11.5)