##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364168_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1345680 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.312093514059807 31.0 31.0 34.0 26.0 34.0 2 30.360934248855596 31.0 31.0 34.0 26.0 34.0 3 30.543101628916236 33.0 31.0 34.0 26.0 34.0 4 33.76895027049522 37.0 35.0 37.0 28.0 37.0 5 33.93780542179419 37.0 35.0 37.0 30.0 37.0 6 33.77006048986386 37.0 35.0 37.0 28.0 37.0 7 33.711887670174185 37.0 35.0 37.0 28.0 37.0 8 33.58859238451935 37.0 35.0 37.0 27.0 37.0 9 34.91568797931158 39.0 35.0 39.0 27.0 39.0 10-11 34.947396483562216 39.0 35.0 39.0 27.0 39.0 12-13 34.8461220349563 39.0 35.0 39.0 26.0 39.0 14-15 36.02507245407526 40.0 37.0 41.0 26.0 41.0 16-17 36.07523965578741 40.0 37.0 41.0 27.0 41.0 18-19 36.009124011652105 40.0 36.0 41.0 27.0 41.0 20-21 36.037696554901615 40.0 36.5 41.0 27.0 41.0 22-23 36.06058907020986 40.0 36.0 41.0 27.0 41.0 24-25 36.05771245764223 40.0 36.0 41.0 27.0 41.0 26-27 35.862490711015994 40.0 36.0 41.0 26.5 41.0 28-29 35.742741216336725 40.0 36.0 41.0 25.5 41.0 30-31 35.77567698115451 40.0 36.0 41.0 26.0 41.0 32-33 35.427843543784554 39.5 35.0 41.0 25.5 41.0 34-35 35.45648259615956 40.0 35.0 41.0 25.0 41.0 36-37 35.467878321740685 40.0 35.0 41.0 25.0 41.0 38-39 35.321347943047385 40.0 35.0 41.0 25.0 41.0 40-41 34.95328569942334 39.0 35.0 41.0 24.5 41.0 42-43 34.7127051007669 39.0 34.0 41.0 24.0 41.0 44-45 34.48194853159741 39.0 33.5 41.0 23.5 41.0 46-47 34.202680429225374 38.0 33.0 40.0 23.0 41.0 48-49 33.90242702574163 38.0 33.0 40.0 19.5 41.0 50-51 33.78163605017538 38.0 33.0 40.0 19.0 41.0 52-53 33.633638383568154 38.0 33.0 40.0 17.5 41.0 54-55 33.307394031270434 38.0 32.5 40.0 13.5 41.0 56-57 32.91242940372154 38.0 32.0 40.0 8.5 41.0 58-59 32.49474206349206 37.0 31.0 40.0 7.5 41.0 60-61 32.14837405623922 37.0 31.0 40.0 4.0 41.0 62-63 31.50734424231615 36.0 29.5 39.0 2.0 41.0 64-65 31.248521936864634 36.0 30.0 39.0 2.0 41.0 66-67 30.808506108435882 35.0 29.5 39.0 2.0 40.5 68-69 30.424467183877297 35.0 29.0 38.0 2.0 40.0 70-71 29.724016482373223 35.0 28.0 37.5 2.0 40.0 72-73 29.44632416324832 34.0 28.0 37.0 2.0 39.0 74-75 29.10102438915641 34.0 28.0 36.5 2.0 39.0 76-77 28.343608064324357 34.0 26.0 36.0 2.0 39.0 78-79 27.857920159324653 34.0 26.0 35.0 2.0 37.0 80-81 27.712039637952557 34.0 26.0 35.0 2.0 37.0 82-83 27.552094108554783 34.0 26.0 35.0 2.0 36.5 84-85 27.27064420961893 34.0 26.0 35.0 2.0 36.0 86-87 26.9071911598597 33.0 25.0 35.0 2.0 36.0 88-89 26.52339932227573 33.0 24.5 35.0 2.0 35.5 90-91 26.296430800784734 33.0 24.0 35.0 2.0 35.0 92-93 25.88732611021937 33.0 21.5 35.0 2.0 35.0 94-95 25.842423533083647 33.0 20.0 35.0 2.0 35.0 96-97 25.78500646513287 33.0 21.5 35.0 2.0 35.0 98-99 25.604831757921644 33.0 20.0 35.0 2.0 35.0 100-101 24.984406768325307 32.5 18.0 35.0 2.0 35.0 102-103 23.748879005409904 30.0 12.0 34.0 2.0 35.0 104-105 24.511206601866714 32.0 12.5 35.0 2.0 35.0 106-107 24.355840170025566 32.0 8.5 35.0 2.0 35.0 108-109 24.282815008025683 32.0 7.5 35.0 2.0 35.0 110-111 24.46162943641876 32.5 7.0 35.0 2.0 35.0 112-113 24.399397702276914 32.5 6.0 35.0 2.0 35.0 114-115 24.234696213066997 32.5 2.0 35.0 2.0 35.0 116-117 23.87776886035313 32.0 2.0 35.0 2.0 35.0 118-119 23.56636235955056 32.0 2.0 35.0 2.0 35.0 120-121 23.30777933832709 32.0 2.0 35.0 2.0 35.0 122-123 22.774417023363654 32.0 2.0 35.0 2.0 35.0 124-125 21.742903587777185 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2924032820188991 1101 2 0.3583956474723564 1101 3 0.20222736912175066 1101 4 0.3440935175429871 1101 5 0.2707090963769758 1101 6 0.22800377499131486 1101 7 0.2233665026925138 1101 8 0.26432449591708007 1101 9 0.5091388004551405 1101 10-11 0.45918526090213163 1101 12-13 0.35064569665279777 1101 14-15 0.511325346734111 1101 16-17 0.45277266862999 1101 18-19 0.5724608039374175 1101 20-21 0.5631785598530783 1101 22-23 0.40275414612994354 1101 24-25 0.5644025346610135 1101 26-27 0.5943629096346896 1101 28-29 0.6496480715719812 1101 30-31 0.6101666303091875 1101 32-33 0.48903867414330193 1101 34-35 0.47434516114351766 1101 36-37 0.40135201208358495 1101 38-39 0.5454018977535995 1101 40-41 0.6733293043443354 1101 42-43 0.726870936750025 1101 44-45 0.6525806486828287 1101 46-47 0.6831419618142078 1101 48-49 0.8908264445000498 1101 50-51 0.8976391917564399 1101 52-53 0.8416885630144293 1101 54-55 0.9884981235721142 1101 56-57 0.9207779019857938 1101 58-59 0.856781385368599 1101 60-61 0.8701249329513061 1101 62-63 0.8420066758226348 1101 64-65 0.7984199314325231 1101 66-67 0.8419535276912136 1101 68-69 0.8891375530863073 1101 70-71 0.9755267999748014 1101 72-73 1.0033609749776033 1101 74-75 0.7908155974416218 1101 76-77 0.7532675764387591 1101 78-79 0.8920583761325034 1101 80-81 0.8605576436280344 1101 82-83 0.7300465540371519 1101 84-85 0.7039043749370215 1101 86-87 0.6129513933813406 1101 88-89 0.554114149086228 1101 90-91 0.6954307940570139 1101 92-93 0.7142519822507474 1101 94-95 0.6727843231330795 1101 96-97 0.5204905626097798 1101 98-99 0.7030940873830396 1101 100-101 0.6319063388977675 1101 102-103 0.5382000135588108 1101 104-105 0.4670227298999947 1101 106-107 0.4965792351161582 1101 108-109 0.7330840218632702 1101 110-111 0.6477116248804791 1101 112-113 0.5633628607141077 1101 114-115 0.49205302528552153 1101 116-117 0.5895149574015264 1101 118-119 0.5894935365866409 1101 120-121 0.6813845696072427 1101 122-123 0.6354271168149097 1101 124-125 0.38572506768588966 1102 1 0.239617233443699 1102 2 0.2136945829681487 1102 3 0.2798773876997487 1102 4 0.25603281005680145 1102 5 0.2949573885532857 1102 6 0.16155113085660133 1102 7 0.2217009927690583 1102 8 0.32084341993557075 1102 9 0.4308530738651726 1102 10-11 0.42211126539089605 1102 12-13 0.3267100005587835 1102 14-15 0.4612794275338743 1102 16-17 0.34794394173856347 1102 18-19 0.4728153882960484 1102 20-21 0.39135987910118075 1102 22-23 0.5190904649624244 1102 24-25 0.5023202266227997 1102 26-27 0.4328460036573816 1102 28-29 0.5463032058593456 1102 30-31 0.4564831543108525 1102 32-33 0.6420128311979738 1102 34-35 0.5728909196015195 1102 36-37 0.3760346087567683 1102 38-39 0.5682147767622681 1102 40-41 0.421691210737265 1102 42-43 0.6262543074897309 1102 44-45 0.7010082540841651 1102 46-47 0.6661726390447882 1102 48-49 0.5395955144885249 1102 50-51 0.5197455810851608 1102 52-53 0.6739357595395887 1102 54-55 0.5470830360352039 1102 56-57 0.6497846401401901 1102 58-59 0.6894290604148878 1102 60-61 0.6192444811103783 1102 62-63 0.6482898695956472 1102 64-65 0.6434844436524401 1102 66-67 0.6884838739229835 1102 68-69 0.6910715676462473 1102 70-71 0.5308663216339404 1102 72-73 0.5818034377690289 1102 74-75 0.6113054506504056 1102 76-77 0.5964217097176103 1102 78-79 0.583854537948362 1102 80-81 0.5919917898864497 1102 82-83 0.664523185320494 1102 84-85 0.6419868151744588 1102 86-87 0.6236209724369743 1102 88-89 0.7064482165258568 1102 90-91 0.711665134207621 1102 92-93 0.7983469633873206 1102 94-95 0.6662823997345129 1102 96-97 0.7166815974993597 1102 98-99 0.7004273185624292 1102 100-101 0.549784080654593 1102 102-103 0.45687958867851464 1102 104-105 0.7204992189690245 1102 106-107 0.5736481279376378 1102 108-109 0.5762472939142498 1102 110-111 0.653803680608462 1102 112-113 0.6877920004276525 1102 114-115 0.7667108095664332 1102 116-117 0.6055780394661987 1102 118-119 0.6052902171843044 1102 120-121 0.5466843263406389 1102 122-123 0.5444705452615821 1102 124-125 0.41058227750108145 1103 1 0.19856243827213405 1103 2 0.17303483104342732 1103 3 0.18558977245405117 1103 4 0.2964341502335941 1103 5 0.28755849586247706 1103 6 0.2699890169891006 1103 7 0.20619628789309985 1103 8 0.35293697092882326 1103 9 0.3929615949182974 1103 10-11 0.42147266164600694 1103 12-13 0.42819601662188944 1103 14-15 0.4068332603444489 1103 16-17 0.4608550243352738 1103 18-19 0.4997986242051766 1103 20-21 0.4531737513566796 1103 22-23 0.432411145030855 1103 24-25 0.495972802423573 1103 26-27 0.5991592105768788 1103 28-29 0.6342092390311009 1103 30-31 0.46935770283285905 1103 32-33 0.4650661293635494 1103 34-35 0.345384622671574 1103 36-37 0.46665099503183427 1103 38-39 0.3963665090120472 1103 40-41 0.3822938150524493 1103 42-43 0.4224372392452693 1103 44-45 0.38518008898579836 1103 46-47 0.47162275072619053 1103 48-49 0.47662027449231914 1103 50-51 0.5510089472100503 1103 52-53 0.6039061758498292 1103 54-55 0.7074869306269704 1103 56-57 0.5160105217103776 1103 58-59 0.6912824964000706 1103 60-61 0.41496702411959774 1103 62-63 0.6012826934717062 1103 64-65 0.594515417419192 1103 66-67 0.614873653886864 1103 68-69 0.6028254408522145 1103 70-71 0.5378107660783868 1103 72-73 0.5236563391092055 1103 74-75 0.4858019932794342 1103 76-77 0.30968714313648604 1103 78-79 0.3508547636873587 1103 80-81 0.47165805259911764 1103 82-83 0.45429334737733384 1103 84-85 0.5321915248028226 1103 86-87 0.48706224465444237 1103 88-89 0.47025690866539094 1103 90-91 0.31027446316874574 1103 92-93 0.24893388478262324 1103 94-95 0.43835581243292765 1103 96-97 0.5301558394915666 1103 98-99 0.40347212181307057 1103 100-101 0.36092964666542926 1103 102-103 0.28038882021544254 1103 104-105 0.3310756850844143 1103 106-107 0.11315839312155518 1103 108-109 0.2538533912433998 1103 110-111 0.37082648024727405 1103 112-113 0.30678764010068704 1103 114-115 0.2867421754061823 1103 116-117 0.31565490953558495 1103 118-119 0.2944268249212101 1103 120-121 0.0760407919807875 1103 122-123 0.060532172008088025 1103 124-125 0.034521898259566086 1104 1 0.2760421547748848 1104 2 0.32379236659851074 1104 3 0.2830449011127456 1104 4 0.28887648377919817 1104 5 0.2604624853433606 1104 6 0.3739154067624426 1104 7 0.30437961768861044 1104 8 0.2355911903679626 1104 9 0.5683040263435686 1104 10-11 0.31736422158845556 1104 12-13 0.4629134480471606 1104 14-15 0.4668121955787683 1104 16-17 0.5100469274461972 1104 18-19 0.4281758412386054 1104 20-21 0.4786659504651425 1104 22-23 0.36453034300722464 1104 24-25 0.5179242340611196 1104 26-27 0.567991759693605 1104 28-29 0.7616440694516342 1104 30-31 0.6650982421144462 1104 32-33 0.695241811573041 1104 34-35 0.6069098060485345 1104 36-37 0.6016925054448237 1104 38-39 0.6552961860091457 1104 40-41 0.6605934211752356 1104 42-43 0.8204127958621683 1104 44-45 0.7768443234017042 1104 46-47 0.911645289583241 1104 48-49 1.047112797900894 1104 50-51 0.8414561717908242 1104 52-53 0.7974005684062604 1104 54-55 0.9538278673235965 1104 56-57 0.9586079296681191 1104 58-59 1.058446226676729 1104 60-61 0.902414767580531 1104 62-63 0.9353958211398172 1104 64-65 0.735595911652549 1104 66-67 0.8623334259333753 1104 68-69 0.8828099334110036 1104 70-71 0.913048094788973 1104 72-73 0.9483682279509225 1104 74-75 1.033658738131905 1104 76-77 1.0248783180462695 1104 78-79 1.1305809217335394 1104 80-81 0.9631577047135558 1104 82-83 0.9039837144850473 1104 84-85 0.9804295433881656 1104 86-87 0.8526932884290623 1104 88-89 0.8706621363830251 1104 90-91 0.6889412654518168 1104 92-93 0.8390745720629837 1104 94-95 0.6999005275205477 1104 96-97 0.7946063571924569 1104 98-99 0.7181578307920553 1104 100-101 0.8021797351109115 1104 102-103 0.6963512397300526 1104 104-105 0.7995743525058145 1104 106-107 0.9497332533776941 1104 108-109 1.0294133985548939 1104 110-111 0.930746451826792 1104 112-113 0.9426800697570457 1104 114-115 0.9006157337448215 1104 116-117 0.8777517455458685 1104 118-119 0.7540020802338319 1104 120-121 0.6306879387295297 1104 122-123 0.8267143785459794 1104 124-125 0.7282285599732852 1105 1 0.15325362847370627 1105 2 0.20922798477550586 1105 3 0.17658224787559007 1105 4 0.1844202270172488 1105 5 0.0905000156076241 1105 6 0.02411517725712997 1105 7 0.08140225263193201 1105 8 0.14739274343390463 1105 9 0.23166889156244963 1105 10-11 0.0842071181737154 1105 12-13 0.26649910746606764 1105 14-15 0.1921656531054694 1105 16-17 0.1418122111315654 1105 18-19 0.167495972752441 1105 20-21 -6.438050776651494E-4 1105 22-23 0.14540185602113098 1105 24-25 0.20263610499951312 1105 26-27 0.3132818694443458 1105 28-29 0.3102739050936094 1105 30-31 0.14737731754149053 1105 32-33 0.06344690324173285 1105 34-35 0.2157315359466878 1105 36-37 0.2873861518396623 1105 38-39 0.37724525757029426 1105 40-41 0.2549595480122946 1105 42-43 0.3106541681737056 1105 44-45 0.33408281183964306 1105 46-47 0.35007695103376335 1105 48-49 0.43398900629877346 1105 50-51 0.2936997735405811 1105 52-53 0.4024984068598769 1105 54-55 0.4918066634220537 1105 56-57 0.39354045525242753 1105 58-59 0.39932747909902844 1105 60-61 0.32559463300628977 1105 62-63 0.4433523644773665 1105 64-65 0.38433097599556376 1105 66-67 0.4593041957114359 1105 68-69 0.5395565058272282 1105 70-71 0.4967379644086982 1105 72-73 0.5575092184962571 1105 74-75 0.6151246782357696 1105 76-77 0.3468920986481052 1105 78-79 0.4817444970316771 1105 80-81 0.5304592691641759 1105 82-83 0.5834701406436622 1105 84-85 0.42450457747487036 1105 86-87 0.5990186693811985 1105 88-89 0.5752045249668143 1105 90-91 0.5887714011596259 1105 92-93 0.5513656417991548 1105 94-95 0.39302898071985126 1105 96-97 0.2701425204384549 1105 98-99 0.25624080281488304 1105 100-101 0.3850996087679164 1105 102-103 0.23916240317803883 1105 104-105 0.333760092180583 1105 106-107 0.2946236262736406 1105 108-109 0.35823460451302225 1105 110-111 0.4788918877828401 1105 112-113 0.46967571199611413 1105 114-115 0.3746273294678062 1105 116-117 0.37919331509388243 1105 118-119 0.42091835290495183 1105 120-121 0.4548228194976751 1105 122-123 0.3682357681524273 1105 124-125 0.2789042100077914 1106 1 0.3480060502157478 1106 2 0.4244646512232464 1106 3 0.40739019425447154 1106 4 0.4166514329508786 1106 5 0.4780506426046216 1106 6 0.5019598239599077 1106 7 0.4434840001808098 1106 8 0.4580145322563851 1106 9 0.6837326294393336 1106 10-11 0.5124365588599034 1106 12-13 0.599787272588145 1106 14-15 0.6327965331827201 1106 16-17 0.6828566338368844 1106 18-19 0.6179454079769684 1106 20-21 0.692174334107257 1106 22-23 0.6619180964128049 1106 24-25 0.7590298842306638 1106 26-27 0.8327887754564429 1106 28-29 0.8374771516740154 1106 30-31 0.6565867931869533 1106 32-33 0.3292443122917348 1106 34-35 0.5543971379340888 1106 36-37 0.6150886684695109 1106 38-39 0.5749202469407848 1106 40-41 0.5989077566663923 1106 42-43 0.5681453505783836 1106 44-45 0.520431296112001 1106 46-47 0.4060921008205369 1106 48-49 0.4810255913976391 1106 50-51 0.4784013464774475 1106 52-53 0.38483982553347573 1106 54-55 0.394004926519969 1106 56-57 0.45049964369950857 1106 58-59 0.43443101454858635 1106 60-61 0.5619370679646423 1106 62-63 0.6125644672534811 1106 64-65 0.58248057725935 1106 66-67 0.6173049031931157 1106 68-69 0.6319227324495102 1106 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26-27 -8.809166297071876 2216 28-29 -9.223250655550608 2216 30-31 -4.3675680328787045 2216 32-33 -1.9377400833550809 2216 34-35 -1.388898632113282 2216 36-37 -1.248077040556069 2216 38-39 -1.406430249696335 2216 40-41 -1.516420439553528 2216 42-43 -1.513653529946616 2216 44-45 -1.6477690810055705 2216 46-47 -1.6332053321074653 2216 48-49 -1.4235740552402234 2216 50-51 -1.5328023118599674 2216 52-53 -1.6349261834045024 2216 54-55 -1.9162989946119815 2216 56-57 -2.0624470698841506 2216 58-59 -2.208915235804035 2216 60-61 -2.078104207108481 2216 62-63 -2.2135368199942995 2216 64-65 -2.1578873350830854 2216 66-67 -2.1494523310800453 2216 68-69 -2.072951586437547 2216 70-71 -2.171873348462416 2216 72-73 -2.1155184789071733 2216 74-75 -1.922156655025553 2216 76-77 -2.005064685448943 2216 78-79 -1.983102801659534 2216 80-81 -1.969066275266929 2216 82-83 -1.858115393474879 2216 84-85 -2.0569291696124594 2216 86-87 -2.1656852754298583 2216 88-89 -2.0440410059111898 2216 90-91 -2.028741240695684 2216 92-93 -2.234023621264054 2216 94-95 -2.118647373252582 2216 96-97 -2.2944171017215034 2216 98-99 -2.2120331597949168 2216 100-101 -2.2484777983311908 2216 102-103 -2.1257514500769012 2216 104-105 -2.219966248919306 2216 106-107 -2.2426122958497707 2216 108-109 -2.2668241574994354 2216 110-111 -2.4608801071320805 2216 112-113 -2.406723647355662 2216 114-115 -2.410829626464981 2216 116-117 -2.400523794103311 2216 118-119 -2.4556040403564694 2216 120-121 -2.3885493219922864 2216 122-123 -2.1560206819850514 2216 124-125 -2.254332625666617 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44678.0 3 8047.0 4 5981.0 5 4650.0 6 5985.0 7 5348.0 8 5187.0 9 5845.0 10 7071.0 11 8329.0 12 9244.0 13 10205.0 14 10717.0 15 10797.0 16 11224.0 17 11350.0 18 12088.0 19 12791.0 20 13675.0 21 14901.0 22 15997.0 23 19331.0 24 22013.0 25 21536.0 26 25131.0 27 26847.0 28 31101.0 29 38270.0 30 45249.0 31 53723.0 32 65811.0 33 77444.0 34 89992.0 35 106611.0 36 147061.0 37 233246.0 38 107588.0 39 616.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.580082930541 17.48037810830851 9.667538584804431 23.272000376346053 2 12.124590095390552 24.5420118625946 35.76682774150773 27.566570300507117 3 22.73999784996327 32.04995550618434 26.042095927472094 19.167950716380293 4 13.580217420841164 19.716436161556135 31.210163028304127 35.49318338929857 5 10.761488546540969 43.196606515227984 32.96919465953171 13.072710278699343 6 31.608320914577874 29.16896874057394 20.800270574567783 18.4224397702804 7 24.9362859049641 34.26638837006878 21.965947484954217 18.8313782400129 8 29.707596924462354 33.353245525543734 21.920262124098258 15.018895425895657 9 31.544551882353993 16.23001968533338 18.48158569990471 33.74384273240791 10-11 23.129085824580873 31.34537635982553 23.73539446656079 21.79014334903281 12-13 27.081554183309926 28.39185499984877 23.19893383559664 21.327656981244665 14-15 28.32836876228932 26.46862389855774 22.080289041445177 23.122718297707763 16-17 23.01270444524918 30.006122624216474 29.159781825512678 17.82139110502167 18-19 25.418914752294373 29.156840040822026 27.9296783137846 17.494566893099 20-21 21.274100875465574 30.025919395644664 28.425061662626756 20.274918066263012 22-23 19.76231396824555 31.99774179974229 22.03470265349732 26.205241578514837 24-25 20.905238470880356 33.628327173991366 20.671943827099884 24.794490528028398 26-27 24.0213268018758 27.935272857612237 22.241703754592432 25.80169658591953 28-29 23.286272445392754 27.896223368409657 25.843427809792622 22.974076376404966 30-31 25.960353279914926 30.334382210094233 23.81525281141166 19.89001169857918 32-33 19.103204515939687 30.826560643546795 26.82239061324985 23.247844227263663 34-35 16.913099792814716 32.509495836692146 27.98700210097604 22.590402269517103 36-37 24.407137821399207 29.533700801331037 24.649205405504258 21.4099559717655 38-39 27.00928415113401 27.506433544414772 23.750989560832718 21.7332927436185 40-41 21.595821352524514 31.905049323379625 23.93015791539149 22.56897140870437 42-43 25.37851047644772 29.762341346069547 23.242145602160242 21.617002575322495 44-45 27.523075131703283 27.328375209180194 23.249977234626655 21.898572424489867 46-47 22.695931480712904 29.295182629518713 29.834165887857445 18.174720001910945 48-49 25.080373851308934 28.9154400650306 27.890491513999272 18.113694569661195 50-51 20.169568373110128 30.323780807502203 28.517937341052974 20.98871347833469 52-53 18.923089093423425 32.05399741632729 22.51489417912419 26.508019311125093 54-55 20.382644998475673 33.24877708757094 21.190407157354098 25.17817075659929 56-57 23.55035952568651 27.880262817697925 22.490231638782735 26.079146017832834 58-59 22.98519624848752 27.63369436373941 25.853528040268653 23.52758134750442 60-61 25.26707428377823 30.113479051599906 24.097731090618314 20.52171557400355 62-63 18.875538123812227 30.63416610912369 26.751950529737346 23.738345237326733 64-65 16.664974831463358 32.00815365048277 28.04060105928788 23.286270458765994 66-67 23.674244332423207 29.377065648101812 24.84964637932958 22.099043640145396 68-69 26.245534636443935 27.427902080496512 23.87344843426339 22.453114848796158 70-71 21.22998308629572 31.078035920348846 24.17614734777293 23.515833645582507 72-73 24.83200261540747 29.245502715799105 23.58845249833365 22.334042170459778 74-75 26.87350758718602 26.923519045034944 23.521135066018857 22.68183830176018 76-77 22.15942762375188 28.767638589718903 29.715267297498617 19.3576664890306 78-79 24.39157178594671 28.67620499011887 27.803284952624317 19.1289382713101 80-81 19.94112593660738 29.98031809011583 28.35142919625111 21.727126777025678 82-83 18.814688541524003 31.19279373145992 22.783427940859816 27.209089786156266 84-85 20.11273460721076 32.41711431498147 21.62690853047585 25.84324254733193 86-87 23.090771618092372 27.28470355710574 22.949441284281097 26.675083540520788 88-89 22.67470128583012 26.903063282514328 26.088780078980474 24.333455352675077 90-91 24.580457522583572 29.35745034002233 24.509930502898698 21.552161634495402 92-93 19.08483750605513 29.793293341483967 26.576181188902897 24.545687963558006 94-95 17.145994044684645 30.76018529472243 28.03715298756579 24.05666767302714 96-97 23.50454756918912 28.40280505871604 25.249445707295397 22.843201664799437 98-99 25.615079527882685 26.87287548327277 24.19817559165716 23.313869397187375 100-101 21.1568514389592 29.658351155128315 24.522802324653306 24.66199508125918 102-103 24.255089065640465 28.379441629097634 24.12532208686184 23.24014721840006 104-105 25.984929365958653 26.286163051775425 24.043131830943686 23.685775751322232 106-107 21.64340429531463 28.014616094413075 29.373433976563824 20.968545633708473 108-109 23.38726462564278 28.35376520640879 27.897433066437095 20.361537101511335 110-111 19.565727520002923 29.713600123559818 28.25744464710278 22.463227709334475 112-113 18.61141462792279 30.7509886731132 23.191076071033006 27.446520627930997 114-115 19.600979030526997 32.44696824716455 22.22560453568235 25.72644818662611 116-117 22.372910053225766 27.693622156717662 23.24096146502136 26.692506325035204 118-119 21.81273893004871 27.422815540684027 25.876495161404094 24.88795036786317 120-121 23.520284859557744 29.598707083977953 24.736350012970217 22.144658043494083 122-123 19.149056822688337 30.125414993913363 26.023471335714266 24.702056847684037 124-125 17.700235504252543 31.138526712553872 27.100111876643968 24.061125906549616 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8162.0 1 5347.0 2 4199.0 3 6012.5 4 5512.0 5 4288.0 6 3254.0 7 2531.5 8 2218.0 9 2012.0 10 1714.5 11 1466.5 12 1321.0 13 1284.0 14 1269.0 15 1262.5 16 1299.0 17 1422.5 18 1555.0 19 1686.0 20 1929.5 21 2291.0 22 2717.5 23 3311.5 24 4081.5 25 4801.0 26 5798.0 27 6957.5 28 8055.0 29 9475.5 30 11179.0 31 12924.5 32 14462.0 33 15739.5 34 17254.0 35 18699.0 36 20359.5 37 21657.0 38 21834.0 39 21784.5 40 21610.5 41 22023.0 42 22334.5 43 23105.5 44 24709.5 45 25919.5 46 29248.0 47 38632.5 48 51283.0 49 85864.5 50 199318.0 51 212226.5 52 96935.0 53 43355.0 54 28912.5 55 22651.5 56 18779.5 57 15993.5 58 14574.5 59 13244.5 60 11454.5 61 9881.0 62 8491.5 63 6513.0 64 4743.0 65 3730.0 66 2687.5 67 1940.0 68 1429.0 69 995.5 70 790.0 71 673.0 72 620.5 73 566.5 74 399.5 75 303.5 76 236.0 77 132.5 78 86.5 79 48.5 80 31.0 81 21.5 82 16.5 83 18.0 84 17.5 85 16.0 86 12.0 87 7.5 88 7.5 89 6.5 90 6.5 91 6.0 92 4.5 93 3.5 94 3.0 95 5.0 96 3.5 97 2.0 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48206111408358604 2 0.4714345163783366 3 0.45835562689495274 4 0.457612508174306 5 0.45865287438321145 6 0.4687592889840081 7 0.4534510433386838 8 0.48057487664229237 9 0.49135009809167113 10-11 0.49606890196777836 12-13 0.49599459009571367 14-15 0.4723634147791451 16-17 0.47448130313298853 18-19 0.46125378990547533 20-21 0.4708028654657868 22-23 0.48752303668034 24-25 0.47459277094108554 26-27 0.4845134058617205 28-29 0.4798689138576779 30-31 0.49257624398073835 32-33 0.49053266749895963 34-35 0.4860367992390464 36-37 0.4437533440342429 38-39 0.40418227215980024 40-41 0.39697402056952624 42-43 0.4057056655371262 44-45 0.44037215385529993 46-47 0.4479519648058974 48-49 0.44609416800428037 50-51 0.4315661970156352 52-53 0.42558409131442837 54-55 0.4268102372034956 56-57 0.40956988288448964 58-59 0.41469740205695266 60-61 0.42335473515248795 62-63 0.42532399976220203 64-65 0.43933178764639436 66-67 0.4277019796682718 68-69 0.4297827120860829 70-71 0.4412638963200761 72-73 0.4404464657273646 74-75 0.44490517805124546 76-77 0.4356161940431603 78-79 0.4274418881160454 80-81 0.4362850008917425 82-83 0.4344643600261578 84-85 0.4315661970156352 86-87 0.43925747577432966 88-89 0.42569555912252544 90-91 0.4286308780690803 92-93 0.438960228286071 94-95 0.4360992212115808 96-97 0.40563135366506153 98-99 0.37757862196064446 100-101 0.3784703644254206 102-103 0.35781166399143927 104-105 0.36119285417038227 106-107 0.3792134831460674 108-109 0.3870905415849236 110-111 0.40414511622376786 112-113 0.3993148445395636 114-115 0.39938915641162837 116-117 0.3697758753938529 118-119 0.44464508649901907 120-121 0.45125884311277575 122-123 0.40496254681647936 124-125 0.3986460376909815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1345680.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.76482369418946 #Duplication Level Percentage of deduplicated Percentage of total 1 86.1453762631339 46.31590966857007 2 9.506032914933883 10.221803674051642 3 2.231666550128704 3.5995547583566943 4 0.823144492939328 1.7702487415090382 5 0.3712045022839644 0.9978872309893347 6 0.2122645375079316 0.6847419261385564 7 0.14369447306078328 0.5407995606959729 8 0.10825264050088204 0.46561473047683166 9 0.06559177733654376 0.31738773098590256 >10 0.34886273781054883 3.4129192706506024 >50 0.021956410893755395 0.823337679572875 >100 0.014687777568185274 1.5618795460650137 >500 0.003316594781231146 1.2335230828284502 >1k 0.0014213977633847769 2.098611068500389 >5k 0.0014213977633847769 5.346851724555977 >10k+ 0.0011055315937437153 20.608929606052694 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 78592 5.840318649307413 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 61735 4.587643421913085 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 38667 2.873417157125022 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 17669 1.3130164675108495 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 17645 1.3112329825812972 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 15298 1.1368230188454909 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 13102 0.9736341477914511 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8696 0.6462160394744665 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 8540 0.6346233874323761 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7780 0.5781463646632186 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7560 0.5617977528089888 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7358 0.5467867546519232 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 6472 0.4809464360026158 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5908 0.4390345401581357 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 5592 0.4155519885856965 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5063 0.37624100826348017 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4983 0.37029605849830566 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4328 0.3216217822959396 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3596 0.26722549194459305 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 2721 0.20220260388799713 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2515 0.18689435824267284 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2149 0.15969621306699958 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 2110 0.156798050056477 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.7155936032340524E-5 0.0 12-13 0.0 0.0 0.0 7.431187206468105E-4 0.0 14-15 0.0 0.0 0.0 0.0017463289935200049 0.0 16-17 0.0 0.0 0.0 0.0033068783068783067 0.0 18-19 0.0 0.0 0.0 0.004458712323880863 0.0 20-21 0.0 0.0 0.0 0.0064651328696272515 0.0 22-23 0.0 0.0 0.0 0.010477973961120029 0.0 24-25 0.0 0.0 0.0 0.013896320076095356 0.0 26-27 0.0 0.0 0.0 0.018466500208073243 0.0 28-29 0.0 0.0 0.0 0.027086677367576244 0.0 30-31 0.0 0.0 0.0 0.04146602461209203 0.0 32-33 0.0 0.0 0.0 0.06680637298614826 0.0 34-35 0.0 0.0 0.0 0.10411093276261815 0.0 36-37 0.0 1.486237441293621E-4 0.0 0.161814101420843 0.0 38-39 0.0 1.486237441293621E-4 0.0 0.25143421913084835 0.0 40-41 0.0 1.486237441293621E-4 0.0 0.39054604363593126 0.0 42-43 0.0 1.486237441293621E-4 0.0 0.49499137982284047 0.0 44-45 0.0 1.486237441293621E-4 0.0 0.5992509363295879 0.0 46-47 0.0 1.486237441293621E-4 0.0 0.7347957909755662 0.0 48-49 0.0 1.486237441293621E-4 0.0 0.8570016645859343 0.0 50-51 0.0 1.486237441293621E-4 0.0 0.9776841448189764 0.0 52-53 0.0 1.486237441293621E-4 0.0 1.141207419297307 0.0 54-55 0.0 1.486237441293621E-4 0.0 1.3048421615837347 0.0 56-57 0.0 1.486237441293621E-4 0.0 1.4885411093276262 0.0 58-59 0.0 1.486237441293621E-4 0.0 1.7050859045241067 0.0 60-61 0.0 1.486237441293621E-4 0.0 1.9024210807918673 0.0 62-63 0.0 1.486237441293621E-4 0.0 2.0884236965697642 0.0 64-65 0.0 1.486237441293621E-4 0.0 2.318084537185661 0.0 66-67 0.0 1.486237441293621E-4 0.0 2.5669921526663098 0.0 68-69 0.0 1.486237441293621E-4 0.0 2.877318530408418 0.0 70-71 0.0 1.486237441293621E-4 0.0 3.3553668034005115 0.0 72-73 0.0 1.486237441293621E-4 0.0 3.727706438380596 0.0 74-75 0.0 1.486237441293621E-4 0.0 4.052337851495155 0.0 76-77 0.0 1.486237441293621E-4 0.0 4.44262380357886 0.0 78-79 0.0 2.2293561619404317E-4 0.0 4.8142946317103625 0.0 80-81 0.0 2.2293561619404317E-4 0.0 5.2160617680280605 0.0 82-83 0.0 2.2293561619404317E-4 0.0 5.729371024314844 0.0 84-85 0.0 2.2293561619404317E-4 0.0 6.207530765115035 0.0 86-87 0.0 2.2293561619404317E-4 0.0 6.674320789489329 0.0 88-89 0.0 2.2293561619404317E-4 0.0 7.231994233398728 0.0 90-91 0.0 2.2293561619404317E-4 0.0 7.7419222995065695 0.0 92-93 0.0 2.600915522263837E-4 0.0 8.25991320373343 0.0 94-95 0.0 2.972474882587242E-4 0.0 8.848128827061412 0.0 96-97 0.0 2.972474882587242E-4 0.0 9.417357767076869 0.0 98-99 0.0 2.972474882587242E-4 0.0 10.094190297841983 0.0 100-101 0.0 2.972474882587242E-4 0.0 11.078525355210747 0.0 102-103 0.0 3.7155936032340527E-4 0.0 11.873550918494738 0.0 104-105 0.0 4.4587123238808634E-4 0.0 12.566954996730278 0.0 106-107 0.0 4.4587123238808634E-4 0.0 13.304611794780335 0.0 108-109 0.0 4.4587123238808634E-4 0.0 13.969034242910647 0.0 110-111 0.0 4.4587123238808634E-4 0.0 14.671021342369656 0.0 112-113 0.0 5.573390404851079E-4 0.0 15.560831698472148 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1445 0.0 48.20398 5 AAGTACT 1715 0.0 40.263367 4 GAACAAA 1540 0.0 35.556454 1 AAAGTAC 2035 0.0 33.054474 3 AAAAGTA 2130 0.0 31.577852 2 AAAAAGT 2150 0.0 31.281773 1 ACCGACT 85 0.0071959696 28.011803 8 GTTAGAC 110 7.731157E-4 27.057865 3 GTACAAA 1325 0.0 26.502539 1 GATATAG 280 9.458745E-11 25.507889 1 TGGGATA 930 0.0 24.962132 6 ATATAGG 290 1.4551915E-10 24.630148 2 GGGATAG 325 2.3646862E-11 23.810034 7 TCGCGCT 100 1.828379E-6 23.798475 60-61 GTACATA 835 0.0 23.522245 1 ATGGGAT 2955 0.0 23.370361 5 ATGGGAG 2375 0.0 23.061583 5 ACCTATC 130 0.0020473136 22.894262 7 CGCAAAA 235 2.898596E-7 22.795988 2 GTCGGGA 395 0.0 22.603617 2 >>END_MODULE