##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364168_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1345680 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02673443909399 33.0 31.0 34.0 30.0 34.0 2 31.839790291897035 33.0 31.0 34.0 30.0 34.0 3 32.08403929611795 34.0 31.0 34.0 30.0 34.0 4 35.52198145175673 37.0 35.0 37.0 33.0 37.0 5 35.67110085607277 37.0 35.0 37.0 33.0 37.0 6 35.83727037631532 37.0 35.0 37.0 35.0 37.0 7 35.80649337138101 37.0 35.0 37.0 35.0 37.0 8 35.87942229950657 37.0 35.0 37.0 35.0 37.0 9 37.531670976755244 39.0 37.0 39.0 35.0 39.0 10-11 37.490223530111166 39.0 37.0 39.0 34.5 39.0 12-13 37.47653565483621 39.0 37.0 39.0 34.5 39.0 14-15 38.732157719517275 40.0 38.0 41.0 34.0 41.0 16-17 38.694034986029365 40.0 38.0 41.0 34.0 41.0 18-19 38.760870340645624 40.0 38.0 41.0 34.0 41.0 20-21 38.81240785327864 40.0 38.0 41.0 34.5 41.0 22-23 38.78237954045538 40.0 38.0 41.0 34.5 41.0 24-25 38.75975269008977 40.0 38.0 41.0 34.5 41.0 26-27 38.535818322335174 40.0 38.0 41.0 34.0 41.0 28-29 38.3681094316628 40.0 38.0 41.0 34.0 41.0 30-31 38.208528773556864 40.0 37.5 41.0 34.0 41.0 32-33 37.856623045597765 40.0 37.0 41.0 33.5 41.0 34-35 37.63035305570418 40.0 37.0 41.0 33.0 41.0 36-37 37.552421080791866 40.0 37.0 41.0 33.0 41.0 38-39 37.45212680577849 40.0 36.5 41.0 33.0 41.0 40-41 37.17934315736282 40.0 36.0 41.0 32.0 41.0 42-43 36.99597155341537 40.0 36.0 41.0 31.5 41.0 44-45 36.745491127162474 40.0 35.5 41.0 31.0 41.0 46-47 36.37299952440402 39.0 35.0 41.0 30.0 41.0 48-49 36.014271595030024 39.0 35.0 41.0 29.5 41.0 50-51 35.85698828844896 39.0 35.0 41.0 28.0 41.0 52-53 35.787174142440996 39.0 35.0 41.0 28.0 41.0 54-55 35.60418190060044 39.0 35.0 41.0 28.0 41.0 56-57 35.28641356043042 39.0 35.0 41.0 27.0 41.0 58-59 35.12735828725997 38.5 35.0 40.0 26.5 41.0 60-61 34.932751099815704 38.0 35.0 40.0 26.0 41.0 62-63 34.53778907318234 38.0 34.0 40.0 26.0 41.0 64-65 34.2437068991142 37.5 34.0 40.0 26.0 41.0 66-67 33.78991810831698 37.0 34.0 39.0 25.0 41.0 68-69 33.45552843172226 36.0 33.5 39.0 24.0 41.0 70-71 32.94147754295226 36.0 33.0 39.0 22.5 40.5 72-73 32.56889639438797 35.0 33.0 37.5 22.5 39.0 74-75 32.212802077759946 35.0 32.5 37.0 21.5 39.0 76-77 31.66661241900006 35.0 32.0 36.5 20.0 39.0 78-79 31.263560801973725 35.0 31.5 36.0 20.0 37.0 80-81 31.01352699007193 35.0 31.5 36.0 18.5 37.0 82-83 30.84302954640033 35.0 32.0 35.0 18.0 36.5 84-85 30.604888606503778 35.0 32.0 35.0 16.0 36.0 86-87 30.285178125557337 35.0 31.0 35.0 11.0 36.0 88-89 30.0817791005291 34.0 31.0 35.0 7.5 35.5 90-91 29.902626181558766 34.0 31.0 35.0 7.0 35.0 92-93 29.650107380655133 34.0 30.5 35.0 3.5 35.0 94-95 29.561817445455084 34.0 30.5 35.0 2.0 35.0 96-97 29.492609684323167 34.0 30.5 35.0 2.0 35.0 98-99 29.352410305570416 34.0 30.0 35.0 2.0 35.0 100-101 29.052899649247962 34.0 30.0 35.0 2.0 35.0 102-103 27.563334150763925 32.5 26.0 34.5 2.0 35.0 104-105 28.45013896320076 34.0 29.0 35.0 2.0 35.0 106-107 28.459690268117235 34.0 29.0 35.0 2.0 35.0 108-109 28.44318560133167 34.0 29.0 35.0 2.0 35.0 110-111 28.415858153498604 34.0 29.0 35.0 2.0 35.0 112-113 28.364163099696807 34.0 29.0 35.0 2.0 35.0 114-115 28.245550205100766 34.0 29.0 35.0 2.0 35.0 116-117 27.930907793829142 34.0 29.0 35.0 2.0 35.0 118-119 27.696010938707566 34.0 29.0 35.0 2.0 35.0 120-121 27.464057576838478 34.0 28.0 35.0 2.0 35.0 122-123 27.03665990428631 34.0 26.0 35.0 2.0 35.0 124-125 25.88765679805006 32.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2364287570336998 1101 2 0.5662536149902557 1101 3 0.3899327769692391 1101 4 0.5652626646760552 1101 5 0.24728117517946657 1101 6 0.23035719506761865 1101 7 0.26410582371071456 1101 8 0.19656110437839658 1101 9 0.28770806936427107 1101 10-11 0.29640434427892615 1101 12-13 0.26318274800291874 1101 14-15 0.29475478403704614 1101 16-17 0.38992306199438076 1101 18-19 0.22291824582612918 1101 20-21 0.19838643219959806 1101 22-23 0.3208241851756881 1101 24-25 0.39315507425789775 1101 26-27 0.3083661247117746 1101 28-29 0.40360773240203685 1101 30-31 0.4685675869266106 1101 32-33 0.4782198798379511 1101 34-35 0.40900494552256816 1101 36-37 0.39097315154760537 1101 38-39 0.37358067981372756 1101 40-41 0.5209264415496051 1101 42-43 0.42889161441102175 1101 44-45 0.4477349373895265 1101 46-47 0.5132200560777633 1101 48-49 0.5421899243364905 1101 50-51 0.575189780460839 1101 52-53 0.6535385491137333 1101 54-55 0.7368570801639365 1101 56-57 0.7181254148665275 1101 58-59 0.7083046924260046 1101 60-61 0.7005854303356855 1101 62-63 0.7415252367032963 1101 64-65 0.6363145993269583 1101 66-67 0.6337489879490761 1101 68-69 0.5741468192543877 1101 70-71 0.641013823260181 1101 72-73 0.6921837475121109 1101 74-75 0.6710159614649882 1101 76-77 0.7173369058548609 1101 78-79 0.692192261031753 1101 80-81 0.6796394040067462 1101 82-83 0.6330198004052399 1101 84-85 0.7373952644659703 1101 86-87 0.7314011371337159 1101 88-89 0.6495719129198285 1101 90-91 0.7146879990615176 1101 92-93 0.6124348742214494 1101 94-95 0.7413070344959571 1101 96-97 0.7372337851279482 1101 98-99 0.7877034063960124 1101 100-101 0.6684100240113828 1101 102-103 0.4390368716012496 1101 104-105 0.7303305481689648 1101 106-107 0.8155710393440501 1101 108-109 0.7578656983311554 1101 110-111 0.7115977579884536 1101 112-113 0.6898042681093663 1101 114-115 0.7410403776208518 1101 116-117 0.8718556313319503 1101 118-119 0.9053271903117732 1101 120-121 0.8038245049648296 1101 122-123 0.7775550380866889 1101 124-125 0.7519400848031381 1102 1 0.28583971935391617 1102 2 0.6673381994590493 1102 3 0.3828914156377152 1102 4 0.5408671506364016 1102 5 0.244195775482126 1102 6 0.17108387016699567 1102 7 0.2377363348910606 1102 8 0.25752798146216094 1102 9 0.26817713624806316 1102 10-11 0.24539196862882307 1102 12-13 0.22412964497665655 1102 14-15 0.285542776541746 1102 16-17 0.37345511980550583 1102 18-19 0.2842789664320904 1102 20-21 0.30581119631912657 1102 22-23 0.3077239431109007 1102 24-25 0.37659098717867323 1102 26-27 0.3076691994453924 1102 28-29 0.38333630836601884 1102 30-31 0.4093859001245974 1102 32-33 0.41493538510438555 1102 34-35 0.506795816364928 1102 36-37 0.3846844244358465 1102 38-39 0.45951430678957905 1102 40-41 0.4859782746621022 1102 42-43 0.47376298556735463 1102 44-45 0.37830605806838946 1102 46-47 0.39308288435998406 1102 48-49 0.44673369680160135 1102 50-51 0.47273162602209595 1102 52-53 0.43918251456967283 1102 54-55 0.37657551083896124 1102 56-57 0.45754033815592265 1102 58-59 0.4473506847364135 1102 60-61 0.504900282818376 1102 62-63 0.6210441717383972 1102 64-65 0.5115649766455732 1102 66-67 0.49118864951906005 1102 68-69 0.47167952604352337 1102 70-71 0.49116086985939944 1102 72-73 0.37023119000794935 1102 74-75 0.3640314608225239 1102 76-77 0.3464457228420592 1102 78-79 0.2794446253447731 1102 80-81 0.38290409890194965 1102 82-83 0.4514618175250398 1102 84-85 0.46396783432875566 1102 86-87 0.44806489942348904 1102 88-89 0.5372084561905375 1102 90-91 0.5722941615483847 1102 92-93 0.4221224483957293 1102 94-95 0.4874850247272349 1102 96-97 0.5716464950818505 1102 98-99 0.5893690164703997 1102 100-101 0.46813658694172133 1102 102-103 0.4506023007029043 1102 104-105 0.4819403491390304 1102 106-107 0.6327560371357208 1102 108-109 0.6321693990080774 1102 110-111 0.632713504418323 1102 112-113 0.5806569080377422 1102 114-115 0.6811119404660602 1102 116-117 0.644142843160175 1102 118-119 0.7267967699683133 1102 120-121 0.6662779723401648 1102 122-123 0.5893201180622114 1102 124-125 0.6930007632455499 1103 1 0.15992488236123137 1103 2 0.5120594712012796 1103 3 0.2898899186317294 1103 4 0.4522378615954352 1103 5 0.16082679914907771 1103 6 0.1700205206755001 1103 7 0.17444030789743437 1103 8 0.1401526131511659 1103 9 0.3159268748660651 1103 10-11 0.22609128428322833 1103 12-13 0.21369070143516922 1103 14-15 0.36868155850164186 1103 16-17 0.3578820991800882 1103 18-19 0.2571100898994345 1103 20-21 0.15128245618035407 1103 22-23 0.2183342700760207 1103 24-25 0.17165561981815358 1103 26-27 0.09028699955945285 1103 28-29 0.19674668998373335 1103 30-31 0.3284926271467441 1103 32-33 0.4353172404413357 1103 34-35 0.429043582736945 1103 36-37 0.3913853083822332 1103 38-39 0.35774611697872416 1103 40-41 0.4612524287349089 1103 42-43 0.445698578666871 1103 44-45 0.5719856631439555 1103 46-47 0.6179115009363585 1103 48-49 0.5773058857570206 1103 50-51 0.5810744641553569 1103 52-53 0.5649473539385497 1103 54-55 0.5925750593609678 1103 56-57 0.5363574064003913 1103 58-59 0.496864751840306 1103 60-61 0.35660906762971223 1103 62-63 0.35898495683487397 1103 64-65 0.3878496097602948 1103 66-67 0.41259137977284155 1103 68-69 0.4683602716950688 1103 70-71 0.44025524064164756 1103 72-73 0.5286539278655695 1103 74-75 0.5771067982429159 1103 76-77 0.543495076515665 1103 78-79 0.42418342156186384 1103 80-81 0.41249818454020115 1103 82-83 0.3563752050357323 1103 84-85 0.3017298103766599 1103 86-87 0.21419632704443714 1103 88-89 0.26706125060171715 1103 90-91 0.34396213512504303 1103 92-93 0.2636848592882295 1103 94-95 0.3213411220563849 1103 96-97 0.2633093004235505 1103 98-99 0.36552949313611194 1103 100-101 0.2376288524107828 1103 102-103 0.05229326401437717 1103 104-105 0.3237062221549003 1103 106-107 0.2577694032606139 1103 108-109 0.33673051487158645 1103 110-111 0.30271279155832787 1103 112-113 0.33438308663292915 1103 114-115 0.45125766092989394 1103 116-117 0.4413118979078199 1103 118-119 0.3933401712609701 1103 120-121 0.5009612842876976 1103 122-123 0.3609955172638095 1103 124-125 0.15825180877354228 1104 1 0.24532194361268012 1104 2 0.5194667803359287 1104 3 0.08627736291107624 1104 4 0.49240464029304576 1104 5 0.17493385356073787 1104 6 0.18634903078187648 1104 7 0.1615818088762282 1104 8 0.12625709085111225 1104 9 0.2928573449243217 1104 10-11 0.2866808618676018 1104 12-13 0.2584629100957514 1104 14-15 0.23205576838866193 1104 16-17 0.2815190068901714 1104 18-19 0.17161647566324945 1104 20-21 0.23056977722566785 1104 22-23 0.19256199360723514 1104 24-25 0.15937349052264693 1104 26-27 0.3241114264327294 1104 28-29 0.34348695803250706 1104 30-31 0.3618494722374308 1104 32-33 0.3806299247027596 1104 34-35 0.5673774762250048 1104 36-37 0.5650391681430804 1104 38-39 0.54002156864032 1104 40-41 0.6296463619644612 1104 42-43 0.6190837852755138 1104 44-45 0.5077996711394235 1104 46-47 0.49725233149840165 1104 48-49 0.4869352402229268 1104 50-51 0.5072902947174001 1104 52-53 0.4975294166051256 1104 54-55 0.5318225946802002 1104 56-57 0.6495259805412843 1104 58-59 0.6271552657675201 1104 60-61 0.4039498805026511 1104 62-63 0.31624515165079004 1104 64-65 0.46735362223701316 1104 66-67 0.45605238415974014 1104 68-69 0.5357634681148014 1104 70-71 0.5008031751448172 1104 72-73 0.3652660089287991 1104 74-75 0.32450686958893726 1104 76-77 0.4051700275758314 1104 78-79 0.32624589650231073 1104 80-81 0.24710771247717744 1104 82-83 0.2279634205596821 1104 84-85 0.24234088021321298 1104 86-87 0.321839638207436 1104 88-89 0.2476364115724934 1104 90-91 0.32866032965793224 1104 92-93 0.24740094044580374 1104 94-95 0.25411076284980183 1104 96-97 0.2955767919917456 1104 98-99 0.23934395515172469 1104 100-101 0.32158904309924097 1104 102-103 0.21775744064590086 1104 104-105 0.3180597151618123 1104 106-107 0.40945672194737526 1104 108-109 0.47051462534589206 1104 110-111 0.42693004904081633 1104 112-113 0.3645300977652717 1104 114-115 0.2889708882465776 1104 116-117 0.501183961517814 1104 118-119 0.44147699347375635 1104 120-121 0.5056920120171249 1104 122-123 0.5594771294480623 1104 124-125 0.6051764119481433 1105 1 0.2521833543774932 1105 2 0.6126835443599568 1105 3 0.20410045578776703 1105 4 0.4973321247909084 1105 5 0.23207000231269603 1105 6 0.15416466853072563 1105 7 0.20958132508862093 1105 8 0.0857144163037944 1105 9 0.2842838254894531 1105 10-11 0.2369353277829802 1105 12-13 0.25352685648542206 1105 14-15 0.29932379088478456 1105 16-17 0.3164025171511966 1105 18-19 0.23201843559065338 1105 20-21 0.23269087739198113 1105 22-23 0.17257062147931634 1105 24-25 0.16323852749102485 1105 26-27 0.16964778799247426 1105 28-29 0.3573794479888974 1105 30-31 0.2756721116856582 1105 32-33 0.13075374837784182 1105 34-35 0.17547181089095432 1105 36-37 0.15473182345885306 1105 38-39 0.06619873822330646 1105 40-41 0.16852075273116895 1105 42-43 0.21987111532859416 1105 44-45 0.3564409893921052 1105 46-47 0.1919309371422102 1105 48-49 0.10001006546812619 1105 50-51 0.008938747123444557 1105 52-53 0.01941799023228441 1105 54-55 0.18351020793019757 1105 56-57 0.23835303308243283 1105 58-59 0.37475169092966354 1105 60-61 0.3829837106241598 1105 62-63 0.3350037957429848 1105 64-65 0.3153714763500659 1105 66-67 0.3173095170277449 1105 68-69 0.28581114910485894 1105 70-71 0.2653045005416885 1105 72-73 0.2400295320287995 1105 74-75 0.1945899434617857 1105 76-77 0.15233542638109654 1105 78-79 0.19891549576409417 1105 80-81 0.1276137650861422 1105 82-83 0.11871764201052315 1105 84-85 0.16688984497873705 1105 86-87 0.24316698393464264 1105 88-89 0.16160215519885313 1105 90-91 0.23294542451923306 1105 92-93 0.17979284660080097 1105 94-95 0.2647969357544149 1105 96-97 0.18719074025437976 1105 98-99 0.15381747989944472 1105 100-101 0.22957305656303006 1105 102-103 0.13262037034710517 1105 104-105 0.18623677359116186 1105 106-107 0.16200352337924784 1105 108-109 0.18271351591513962 1105 110-111 0.20575489075094566 1105 112-113 0.23429537998164207 1105 114-115 0.4675537627039361 1105 116-117 0.2907388010220089 1105 118-119 0.1391496470120508 1105 120-121 0.27882573889306883 1105 122-123 0.10446900960192096 1105 124-125 0.17685214106332126 1106 1 0.2493965218328711 1106 2 0.5321435537853638 1106 3 0.41947907947088936 1106 4 0.5422887828963567 1106 5 0.23016579348806943 1106 6 0.12833859149357352 1106 7 0.09829284424996843 1106 8 0.03322134996989945 1106 9 0.16029639423558706 1106 10-11 0.25509145078338946 1106 12-13 0.24328922790869711 1106 14-15 0.38922178216685666 1106 16-17 0.4296235521715417 1106 18-19 0.30615479519413924 1106 20-21 0.17924858727148774 1106 22-23 0.27988591287765274 1106 24-25 0.2558991540616873 1106 26-27 0.35016358131102976 1106 28-29 0.3778888117508572 1106 30-31 0.3044558999850153 1106 32-33 0.29299151061560735 1106 34-35 0.2480336204769884 1106 36-37 0.3331602001571312 1106 38-39 0.2885656446732554 1106 40-41 0.2861736880311696 1106 42-43 0.19757898992228462 1106 44-45 0.2974237667070554 1106 46-47 0.2916797465796037 1106 48-49 0.24434162779276392 1106 50-51 0.24530745338834237 1106 52-53 0.3110904469566407 1106 54-55 0.3063994294353378 1106 56-57 0.4789942834122698 1106 58-59 0.45394239641795053 1106 60-61 0.3922675064131411 1106 62-63 0.3953291681790887 1106 64-65 0.41339732593301903 1106 66-67 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120-121 -2.088736007539371 2216 122-123 -1.9815066262932675 2216 124-125 -2.2608206483164324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24.0 3 8.0 4 40.0 5 208.0 6 588.0 7 1042.0 8 1757.0 9 3221.0 10 6611.0 11 8670.0 12 7935.0 13 9872.0 14 13925.0 15 13776.0 16 8690.0 17 7860.0 18 8025.0 19 8332.0 20 8486.0 21 8543.0 22 8929.0 23 9484.0 24 10600.0 25 12060.0 26 14177.0 27 17140.0 28 21101.0 29 25913.0 30 32091.0 31 40325.0 32 51552.0 33 68724.0 34 96306.0 35 151980.0 36 226896.0 37 316458.0 38 123646.0 39 685.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.34277407049372 18.151130678228334 11.037535972066253 23.468559279211696 2 12.123200532669081 24.133669620324653 35.467378477434394 28.27575136957187 3 23.241832921273083 29.247137314526444 28.60827672613859 18.90275303806188 4 13.901150347779561 19.56676178586291 32.28806254087153 34.244025325486 5 10.640791272813745 39.914838594613876 35.58973901670531 13.854631115867072 6 32.37380399442638 28.980733859730606 19.49235485369252 19.153107292150487 7 21.869686701147376 33.432539682539684 24.46027287319422 20.23750074311872 8 28.631249628440642 31.796786754651922 23.579231317995365 15.992732298912074 9 29.323093157362823 13.743534867130371 21.50243742940372 35.43093454610309 10-11 23.398727780750253 27.67697372332204 26.007260269900716 22.91703822602699 12-13 27.859500256760683 25.616652955423223 26.12850221943786 20.39534456837823 14-15 28.64627224369864 22.634510003254903 26.208884549017508 22.510333204028946 16-17 21.910846295839413 26.87704915596325 32.9488301790327 18.26327436916464 18-19 24.35263212650853 24.6445291599786 33.53761666963914 17.46522204387373 20-21 20.28679212754012 25.042794397027222 34.38440162341897 20.286011852013687 22-23 18.87142559895369 26.824876642292374 28.867078354437904 25.436619404316037 24-25 20.64060549313358 28.357373223946258 27.16790024374294 23.83412103917722 26-27 23.03972712680578 23.355441115272576 29.004146602461212 24.600685155460436 28-29 22.07299528266834 22.369054661003094 32.95603845204938 22.601911604279188 30-31 24.620080554069318 25.087093514059806 30.68054812436835 19.612277807502526 32-33 18.491840556447297 25.313633256048984 33.07472801854824 23.119798168955473 34-35 16.41935675643541 27.055466381309078 34.73251441650318 21.792662445752335 36-37 23.513205219665895 24.83205516913382 30.431046013911185 21.223693597289103 38-39 25.23430533261994 22.866320373342848 30.26871172938589 21.630662564651328 40-41 21.04759010135404 25.984280054901653 30.396216333910242 22.57191350983407 42-43 24.641333749479816 24.876419356756436 29.498469175435467 20.98377771832828 44-45 26.83026425301706 21.997317341418466 29.368646334938468 21.803772070626003 46-47 21.96450864990191 24.761830450032697 35.118936151239524 18.154724748825874 48-49 24.50010403662089 24.507163664467036 32.794869508352654 18.19786279055942 50-51 19.972578919208132 25.604415611438085 32.94193270316866 21.481072766185125 52-53 18.781062362523038 27.355463408834197 27.518020628975687 26.345453599667085 54-55 20.654316033529515 28.518629986326616 25.849384697699307 24.977669282444566 56-57 23.14420961892872 23.80443344628738 27.393585399203374 25.657771535580526 58-59 22.3640835859937 22.74385440818025 31.144068426371796 23.747993579454256 60-61 24.659354378455504 25.392366684501518 29.098002496878898 20.85027644016408 62-63 18.860093038463823 25.66419951251412 31.3885544854646 24.087152963557457 64-65 16.782853278639795 27.077611319184353 33.179619225967535 22.95991617620831 66-67 23.383085131680637 25.29323464716723 29.0419341894061 22.28174603174603 68-69 25.105708638012008 23.38267641638428 28.826243980738365 22.68537096486535 70-71 21.182710599845432 26.06934783901076 29.118661197312885 23.629280363830926 72-73 24.50853843410023 25.07921645562095 28.354066345639378 22.05817876463944 74-75 26.43700582605077 22.347363414779146 28.330175078770587 22.8854556803995 76-77 21.771223470661674 24.870660186671422 34.038330063610964 19.319786279055943 78-79 23.95933654360621 24.601576897925213 32.11376404494382 19.32532251352476 80-81 19.739611200285356 25.644581178289044 32.23935110873313 22.376456512692467 82-83 18.705895903929612 26.980597170203914 27.26235063313715 27.051156292729328 84-85 20.339419475655433 27.921608406158967 25.807287022174663 25.931685096010938 86-87 22.62161137863385 23.508412103917724 27.335176267760534 26.53480024968789 88-89 21.950835265442006 22.596939837108376 30.673005469353782 24.779219428095832 90-91 23.852847630937518 25.078919208132692 28.719606444325546 22.348626716604244 92-93 18.719123417157125 25.371224956899113 30.547975744604962 25.3616758813388 94-95 16.889238983627084 26.604231728863187 32.30170157189647 24.204827715613256 96-97 22.919903198514017 24.71436464642674 28.688271495990197 23.677460659069048 98-99 24.235170667651584 23.154076129566363 28.46609796028171 24.14465524250034 100-101 20.824527376493666 25.393741454134712 28.762112835146542 25.019618334225076 102-103 23.724987626650336 24.45586987249133 28.247614135790265 23.571528365068065 104-105 25.418787828784772 21.91016455683971 28.25544032092263 24.415607293452883 106-107 21.277058296044117 24.31997734589467 33.26734557135653 21.135618786704686 108-109 23.270043142774004 24.34420865211628 31.108396544861762 21.277351660247962 110-111 19.375776451758075 25.18700804592618 31.66050212436086 23.776713377954884 112-113 18.391267622487366 26.453324493831364 27.06708520519326 28.088322678488016 114-115 19.69518262761779 27.411557723753727 25.791492903318375 27.10176674531011 116-117 21.79795036112154 23.383614040435205 27.036238537225444 27.78219706121781 118-119 21.13152754322955 22.770586066646054 29.86049996432224 26.237386425802157 120-121 22.968523664665657 24.71595786507042 28.232491283825112 24.083027186438812 122-123 18.45088404556372 25.210533043209438 29.577328719887756 26.76125419133909 124-125 16.912247743101485 26.559095516522845 30.91560819587828 25.613048544497392 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2852.0 1 2076.5 2 3299.0 3 4344.0 4 2209.0 5 1230.5 6 2268.5 7 4857.0 8 7031.5 9 6970.0 10 4912.5 11 2693.5 12 2028.0 13 2024.5 14 2269.0 15 2648.5 16 2709.0 17 2945.0 18 3212.5 19 3012.5 20 3055.5 21 3408.0 22 3792.0 23 4235.0 24 4752.5 25 5364.0 26 6145.0 27 7157.0 28 8162.0 29 9192.0 30 10745.5 31 12439.5 32 13875.5 33 15235.0 34 16325.5 35 17336.0 36 18884.0 37 20279.5 38 20443.0 39 20138.5 40 19890.0 41 20282.5 42 20876.5 43 21524.0 44 22646.0 45 23960.0 46 27693.0 47 38691.5 48 51035.5 49 90603.5 50 231686.5 51 227635.0 52 77169.5 53 33028.5 54 25596.0 55 19748.5 56 16630.0 57 14534.0 58 13347.0 59 12202.0 60 10826.0 61 9328.0 62 7779.5 63 6117.0 64 4527.0 65 3461.5 66 2418.5 67 1747.5 68 1294.5 69 851.0 70 661.5 71 577.5 72 542.0 73 533.0 74 402.0 75 307.0 76 219.0 77 94.5 78 40.0 79 15.0 80 6.5 81 4.5 82 3.5 83 4.5 84 2.5 85 2.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14334760121276974 2 2.972474882587242E-4 3 2.2293561619404317E-4 4 0.0 5 0.0 6 0.004087152963557458 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005127519172462992 14-15 0.001337613697164259 16-17 2.972474882587242E-4 18-19 0.0 20-21 1.1146780809702159E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.972474882587242E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.431187206468105E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 9.288984008085131E-4 96-97 0.0029353189465549014 98-99 0.003938529219428096 100-101 0.0 102-103 0.003418346114975329 104-105 0.022962368467986445 106-107 0.01698026276677962 108-109 0.019581178289043457 110-111 0.01608852030200345 112-113 0.015196777837227275 114-115 0.04306372986148267 116-117 0.04198620771654479 118-119 0.02259080910766304 120-121 0.014342191308483444 122-123 0.0035669698591046907 124-125 0.0020807324178110697 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1345680.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.626370672542365 #Duplication Level Percentage of deduplicated Percentage of total 1 86.24300270284517 41.936842173416224 2 9.28180394627408 9.026808784027796 3 2.2648184542920626 3.303897049932609 4 0.8294238100698077 1.6132747853234735 5 0.3957440233639222 0.9621797785768672 6 0.22369590863498798 0.6526512102729656 7 0.13834299541342834 0.47089824424462357 8 0.10553616314645979 0.4105472470814127 9 0.06704602798765819 0.2934184508144565 >10 0.3866855075480742 3.5127138359210512 >50 0.02836744654223773 0.9492807198498648 >100 0.02549453941696051 2.683732986615816 >500 0.004620885071284424 1.5494786249962933 >1k 0.0019120903743245889 2.21992759699814 >5k 0.0019120903743245889 7.254377198846283 >10k+ 0.0015934086452704908 23.15997131308216 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 71355 5.302523631175317 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 65151 4.841492776886035 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 39347 2.9239492301290055 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 22654 1.6834611497532848 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 21160 1.5724392128886509 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 20187 1.5001337613697163 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 18371 1.3651834017002558 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 17600 1.3078889483383866 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 12927 0.960629570180132 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 10157 0.7547856845609655 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9802 0.7284049699780036 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 9553 0.7099013138338981 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8726 0.6484453956364069 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 8289 0.6159711075441413 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8267 0.6143362463587183 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 8213 0.6103234052672255 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7245 0.5383895131086143 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 6998 0.520034480708638 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 6979 0.5186225551394091 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 6852 0.5091849473871946 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6728 0.4999702752511741 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5975 0.4440134355864693 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3966 0.2947208846085251 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3351 0.2490190832887462 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3077 0.2286576303430236 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3068 0.2279888234944415 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2997 0.2227126805778491 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2311 0.1717347363414779 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 2118 0.15739254503299446 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 2116 0.1572439212888651 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1768 0.1313833898103561 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT 1348 0.10017240354319006 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.7155936032340524E-5 0.0 12-13 0.0 0.0 0.0 7.0596278461447E-4 0.0 14-15 0.0 0.0 0.0 0.0017091730574876644 0.0 16-17 0.0 0.0 0.0 0.003381190178942988 0.0 18-19 0.0 0.0 0.0 0.004644492004042566 0.0 20-21 0.0 0.0 0.0 0.006650912549788954 0.0 22-23 0.0 0.0 0.0 0.010700909577314073 0.0 24-25 0.0 0.0 0.0 0.014044943820224719 0.0 26-27 0.0 0.0 0.0 0.018615123952202606 0.0 28-29 0.0 1.486237441293621E-4 0.0 0.027235301111705607 0.0 30-31 0.0 1.486237441293621E-4 0.0 0.04187473990844777 0.0 32-33 0.0 1.486237441293621E-4 0.0 0.06762380357885976 0.0 34-35 0.0 1.486237441293621E-4 0.0 0.10585726175613816 0.0 36-37 0.0 1.486237441293621E-4 0.0 0.16419208132691276 0.0 38-39 0.0 2.2293561619404317E-4 0.0 0.25392366684501516 0.0 40-41 0.0 2.2293561619404317E-4 0.0 0.3937786100707449 0.0 42-43 0.0 2.2293561619404317E-4 0.0 0.4990785327863979 0.0 44-45 0.0 2.2293561619404317E-4 0.0 0.6038582723975983 0.0 46-47 0.0 2.2293561619404317E-4 0.0 0.7407778966767731 0.0 48-49 0.0 3.7155936032340527E-4 0.0 0.8636525771357232 0.0 50-51 0.0 3.7155936032340527E-4 0.0 0.9855240473218001 0.0 52-53 0.0 3.7155936032340527E-4 0.0 1.14919594554426 0.0 54-55 0.0 3.7155936032340527E-4 0.0 1.3136852743594316 0.0 56-57 0.0 3.7155936032340527E-4 0.0 1.498907615480649 0.0 58-59 0.0 3.7155936032340527E-4 0.0 1.7167528684382618 0.0 60-61 0.0 3.7155936032340527E-4 0.0 1.9156114380833482 0.0 62-63 0.0 3.7155936032340527E-4 0.0 2.1023571725818915 0.0 64-65 0.0 3.7155936032340527E-4 0.0 2.33395012187147 0.0 66-67 0.0 3.7155936032340527E-4 0.0 2.583043517032281 0.0 68-69 0.0 3.7155936032340527E-4 0.0 2.89496760002378 0.0 70-71 0.0 3.7155936032340527E-4 0.0 3.3733131205041316 0.0 72-73 0.0 3.7155936032340527E-4 0.0 3.7458013792283458 0.0 74-75 0.0 3.7155936032340527E-4 0.0 4.071101599191486 0.0 76-77 0.0 3.7155936032340527E-4 0.0 4.4645829617739725 0.0 78-79 0.0 3.7155936032340527E-4 0.0 4.8386689257475775 0.0 80-81 0.0 3.7155936032340527E-4 0.0 5.244114499732477 0.0 82-83 0.0 3.7155936032340527E-4 0.0 5.760990725878367 0.0 84-85 0.0 3.7155936032340527E-4 0.0 6.242271565305273 0.0 86-87 0.0 3.7155936032340527E-4 0.0 6.712740027346769 0.0 88-89 0.0 3.7155936032340527E-4 0.0 7.2750951191962425 0.0 90-91 0.0 3.7155936032340527E-4 0.0 7.791488318173712 0.0 92-93 0.0 3.7155936032340527E-4 0.0 8.31501545686939 0.0 94-95 0.0 3.7155936032340527E-4 0.0 8.908358599369835 0.0 96-97 0.0 3.7155936032340527E-4 0.0 9.482157719517271 0.0 98-99 0.0 3.7155936032340527E-4 0.0 10.167759051186017 0.0 100-101 0.0 3.7155936032340527E-4 0.0 11.160119790737768 0.0 102-103 0.0 3.7155936032340527E-4 0.0 11.962279293739968 0.0 104-105 0.0 3.7155936032340527E-4 0.0 12.663857677902623 0.0 106-107 0.0 3.7155936032340527E-4 0.0 13.413701622971285 0.0 108-109 0.0 3.7155936032340527E-4 0.0 14.087301587301587 0.0 110-111 0.0 3.7155936032340527E-4 0.0 14.800546935378396 0.0 112-113 0.0 3.7155936032340527E-4 0.0 15.706965994887343 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1375 0.0 53.219933 5 AAAGTAC 1535 0.0 46.897415 3 AAGTACT 1710 0.0 43.489643 4 GTACCGT 70 5.4546115E-5 42.49717 6 AAAAGTA 1925 0.0 37.70518 2 GTTATCA 675 0.0 37.081768 1 AAAAAGT 1935 0.0 36.95857 1 ATAACGC 90 2.3946661E-4 33.052128 3 TATAACG 95 3.2886112E-4 31.312542 2 TTATCAA 865 0.0 28.887178 2 GCAACGC 85 0.007214451 27.997095 4 TCGTAGC 90 0.00953262 26.442684 7 GTACGTG 140 1.0445814E-4 25.54101 1 TTACTCT 845 0.0 24.642416 4 ATTTACT 780 0.0 24.407726 2 ACTTGAC 330 2.910383E-11 23.437834 8 TTTACTC 845 0.0 23.234278 3 CCAAGAC 565 0.0 23.16574 3 GTATAAC 185 2.6577243E-5 22.54972 1 GTCGGGA 425 0.0 22.397678 2 >>END_MODULE