FastQCFastQC Report
Thu 26 May 2016
SRR1364167_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364167_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350203
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA397162.9414836139454588No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT287362.1282725634589763No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG199601.4782962265674124No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC112670.8344671134636791No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC102400.758404476956428No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG93410.6918218964111322No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC90740.6720470921779911No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG75690.5605823716878128No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT68820.5097011338295057No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49160.36409339928884765No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC45900.33994888176074267No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT44120.3267656789386485No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG39370.2915857837673298No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG37120.27492162289670513No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA37110.2748475599595024No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG33360.2470739585084613No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG28940.21433814026483425No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC28870.21381969970441483No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA25500.18886048986707923No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC18880.13983082543884143No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG13990.10361404914668386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTC9450.028.3501345
CATGGGG50350.023.8841694
GTGTAGC5800.023.6053031
AAGTACT13150.021.2779774
ATGGGGA27300.020.9354825
GTATAGG3751.6189006E-1020.6358221
ATGGGAG19150.020.2077985
CGGTTAC1551.9456456E-719.20206828-29
GCACCGC1550.005675310319.2013556
GCGTTAT4300.018.670801104-105
GAACCGT2251.2100148E-418.518646
GTAGCCC5500.018.4011293
ATGGGGG21800.018.297545
TAGACTT5251.8189894E-1218.1440875
TTAGTCG1201.9941885E-417.34959838-39
CTACACG2752.851932E-517.3187084
ATTAGAC3455.968559E-717.255963
AAAGTAC14600.017.1259523
TAGACAG7000.017.010085
GCGCTTA4200.016.990469102-103
Produced by FastQC (version 0.11.5)