FastQCFastQC Report
Thu 26 May 2016
SRR1364167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364167_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350203
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA357072.6445652986995287No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG195161.4454122824493798No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC122850.9098631835361052No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC98800.7317418195634285No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG97190.7198176866737817No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG83980.6219805466289143No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT63280.46867026661916766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52940.3920891895514971No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT49780.3686853013954198No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48880.36201963704716994No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC48290.35764992375220617No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA40730.3016583432269074No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG39670.2938076718834131No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG39050.289215769776841No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC36380.26944096554369973No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG34020.2519621123638445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33490.2480367766920974No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG33040.2447039445179725No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA27820.20604309129812334No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC19710.14597804922667185No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19560.14486710516863022No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG15870.11753788134080578No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14880.11020565055773095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTC10700.037.2582745
AAAGTAC12300.030.9592533
GTACGGG1454.021009E-628.766661
AAGTACT14250.027.9763874
GTTATCA8000.023.8352361
CATGGGG36750.020.8864234
CGTGTAC1754.784112E-420.3999373
TTATCAA9900.019.8332732
GAGTACT78050.019.74396912-13
ATAGCCC3101.9522486E-719.1941995
ATGGGGA20850.019.1205545
AAAAGTA19350.019.064542
AAAAAGT19100.019.030751
AGTACTT80100.018.83011812-13
GCACCGT3804.26553E-918.7908066
ACCGTGC1908.322894E-418.7894158
TAGTACA3554.0059604E-818.4372444
CGTACAG1650.00815859118.0309145
GTGAGCG1650.00815859118.0309149
GTGCTAT2351.6743861E-417.7496431
Produced by FastQC (version 0.11.5)