##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364167_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1350203 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.110192319229036 33.0 31.0 34.0 30.0 34.0 2 31.932850097355733 33.0 31.0 34.0 30.0 34.0 3 32.16559806192106 34.0 31.0 34.0 30.0 34.0 4 35.630001562727976 37.0 35.0 37.0 33.0 37.0 5 35.76630477046785 37.0 35.0 37.0 33.0 37.0 6 35.88464030964233 37.0 35.0 37.0 35.0 37.0 7 35.860840184772215 37.0 35.0 37.0 35.0 37.0 8 35.90511278674392 37.0 35.0 37.0 35.0 37.0 9 37.58568822614081 39.0 37.0 39.0 35.0 39.0 10-11 37.541885923820345 39.0 37.0 39.0 35.0 39.0 12-13 37.52541099375428 39.0 37.0 39.0 34.5 39.0 14-15 38.83695785004181 40.0 38.0 41.0 34.5 41.0 16-17 38.82192936913931 40.0 38.0 41.0 34.5 41.0 18-19 38.9124183548696 40.0 38.5 41.0 35.0 41.0 20-21 38.96595215682383 40.0 39.0 41.0 35.0 41.0 22-23 38.938913259709835 40.0 38.5 41.0 35.0 41.0 24-25 38.91465616651718 40.0 38.0 41.0 35.0 41.0 26-27 38.759428026748566 40.0 38.0 41.0 34.5 41.0 28-29 38.62635285212668 40.0 38.0 41.0 34.0 41.0 30-31 38.52001847129654 40.0 38.0 41.0 34.0 41.0 32-33 38.30240860078077 40.0 38.0 41.0 34.0 41.0 34-35 38.14416054474772 40.0 38.0 41.0 34.0 41.0 36-37 38.04228512305187 40.0 38.0 41.0 33.0 41.0 38-39 37.95765155313683 40.0 37.5 41.0 33.0 41.0 40-41 37.771161817889606 40.0 37.0 41.0 33.0 41.0 42-43 37.62499564880244 40.0 37.0 41.0 33.0 41.0 44-45 37.41098745892285 40.0 37.0 41.0 33.0 41.0 46-47 37.10054525134369 40.0 37.0 41.0 31.5 41.0 48-49 36.7938054499953 40.0 36.5 41.0 31.0 41.0 50-51 36.611840219581794 40.0 36.0 41.0 30.5 41.0 52-53 36.49364243747051 40.0 36.0 41.0 30.0 41.0 54-55 36.32165311438354 39.5 35.5 41.0 30.0 41.0 56-57 36.086633639534206 39.0 35.0 41.0 29.0 41.0 58-59 35.94441058122371 39.0 35.0 41.0 29.0 41.0 60-61 35.79333515034406 39.0 35.0 41.0 29.0 41.0 62-63 35.49378463830995 38.5 35.0 40.5 28.5 41.0 64-65 35.22059979129064 38.0 35.0 40.0 28.0 41.0 66-67 34.837463699902905 37.0 35.0 40.0 28.0 41.0 68-69 34.53119493883512 37.0 34.5 39.5 28.0 41.0 70-71 34.16183344282304 36.0 34.0 39.0 27.5 41.0 72-73 33.88488656890853 36.0 34.0 39.0 28.0 40.5 74-75 33.55164075327932 35.0 34.0 37.5 27.5 39.5 76-77 33.145957681918944 35.0 34.0 37.0 26.5 39.0 78-79 32.779994193465726 35.0 34.0 36.5 26.0 39.0 80-81 32.53568759660584 35.0 34.0 36.0 26.0 38.0 82-83 32.30990784348724 35.0 33.5 36.0 26.0 37.0 84-85 32.07174661884176 35.0 33.0 35.0 26.0 37.0 86-87 31.841644552707997 35.0 33.0 35.0 25.0 36.0 88-89 31.6715412423169 35.0 33.0 35.0 25.0 36.0 90-91 31.52459370924224 35.0 33.0 35.0 25.0 36.0 92-93 31.37388118675488 35.0 33.0 35.0 24.0 36.0 94-95 31.301328763156356 35.0 33.0 35.0 24.0 35.0 96-97 31.22492284493517 35.0 33.0 35.0 24.0 35.0 98-99 31.152360422840122 35.0 33.0 35.0 23.5 35.0 100-101 30.99842912510193 35.0 33.0 35.0 21.5 35.0 102-103 29.75699950303769 33.5 30.0 34.5 18.5 35.0 104-105 30.644074261425875 34.5 32.0 35.0 19.0 35.0 106-107 30.74435584871312 35.0 32.0 35.0 19.0 35.0 108-109 30.697067774253206 35.0 32.5 35.0 18.5 35.0 110-111 30.690886111199575 35.0 33.0 35.0 18.5 35.0 112-113 30.62213274596487 35.0 33.0 35.0 17.5 35.0 114-115 30.516571211884436 35.0 33.0 35.0 12.5 35.0 116-117 30.363393874846967 35.0 32.0 35.0 4.5 35.0 118-119 30.229193313894278 35.0 32.0 35.0 2.0 35.0 120-121 30.071798462897803 35.0 32.0 35.0 2.0 35.0 122-123 29.83099059919138 35.0 32.0 35.0 2.0 35.0 124-125 28.84090244207723 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2795436099785391 1101 2 0.5889835922249596 1101 3 0.3724096794269087 1101 4 0.5008650874206211 1101 5 0.21533363757762913 1101 6 0.22935905771306864 1101 7 0.2460166873953895 1101 8 0.19692907220269262 1101 9 0.29268269112427703 1101 10-11 0.2087061125196925 1101 12-13 0.21686965587726803 1101 14-15 0.3249308183338542 1101 16-17 0.38241189947777343 1101 18-19 0.33566131762424334 1101 20-21 0.2815574036970503 1101 22-23 0.16357587791434725 1101 24-25 0.1925130694498094 1101 26-27 0.18137002624760612 1101 28-29 0.23159411811641917 1101 30-31 0.17733451425208102 1101 32-33 0.11577264941434606 1101 34-35 0.19035942489661295 1101 36-37 0.27102983611837317 1101 38-39 0.32274277386355976 1101 40-41 0.27048013589651987 1101 42-43 0.2963520577073666 1101 44-45 0.21746301854578576 1101 46-47 0.3507665705246126 1101 48-49 0.35122450092736557 1101 50-51 0.4345467148262898 1101 52-53 0.4685170912119432 1101 54-55 0.47427336507603 1101 56-57 0.3087963280547328 1101 58-59 0.3356481281835002 1101 60-61 0.3339530753588562 1101 62-63 0.3553238281503326 1101 64-65 0.46507010269703386 1101 66-67 0.44681493874038125 1101 68-69 0.5411604997142945 1101 70-71 0.49573848653675157 1101 72-73 0.4843431798400317 1101 74-75 0.41103989312068023 1101 76-77 0.4199051333204551 1101 78-79 0.3907943669791365 1101 80-81 0.43568219861684554 1101 82-83 0.31747945850975867 1101 84-85 0.35242912057362474 1101 86-87 0.2570839450981417 1101 88-89 0.25318791343479674 1101 90-91 0.27772659247018794 1101 92-93 0.28737421498308535 1101 94-95 0.29798241405388026 1101 96-97 0.21054480539761045 1101 98-99 0.20886058276061092 1101 100-101 0.13383700051570102 1101 102-103 -0.0338067870549672 1101 104-105 0.14689247931646676 1101 106-107 0.25790941349512764 1101 108-109 0.2636401445678622 1101 110-111 0.3079722974023653 1101 112-113 0.18183729710329644 1101 114-115 0.2602621362186568 1101 116-117 0.26028745020548527 1101 118-119 0.08118680640039955 1101 120-121 0.10920843965127247 1101 122-123 0.12127958648299142 1101 124-125 0.18764644833738942 1102 1 0.4344661593178145 1102 2 0.6926402234778486 1102 3 0.4871452874842248 1102 4 0.5484382019312193 1102 5 0.3623039366474501 1102 6 0.3186078961229839 1102 7 0.28954206802558957 1102 8 0.20028194651547437 1102 9 0.29230851420029325 1102 10-11 0.3434949997480885 1102 12-13 0.3420026839818604 1102 14-15 0.5182039550346644 1102 16-17 0.6021787153623421 1102 18-19 0.43543579253118025 1102 20-21 0.38799304866258666 1102 22-23 0.36750583946638926 1102 24-25 0.34451975595181494 1102 26-27 0.42936342465715427 1102 28-29 0.5263187329575914 1102 30-31 0.506692854604772 1102 32-33 0.6036225133294337 1102 34-35 0.5837017362118075 1102 36-37 0.5761552674690833 1102 38-39 0.597338113690931 1102 40-41 0.7138189375340716 1102 42-43 0.7664228569628619 1102 44-45 0.9461254520883386 1102 46-47 0.8729637249685425 1102 48-49 0.8088970808348037 1102 50-51 0.8105338116662253 1102 52-53 0.7977257041873358 1102 54-55 0.7277772404394511 1102 56-57 0.7122282550262611 1102 58-59 0.9218419903233581 1102 60-61 0.938634872132674 1102 62-63 0.9115399484394402 1102 64-65 0.8957986891347289 1102 66-67 0.8908904896465515 1102 68-69 0.8747797019532442 1102 70-71 0.7750332564377516 1102 72-73 0.7383310510733523 1102 74-75 0.7063922874001278 1102 76-77 0.7458125265130491 1102 78-79 0.7328355219121505 1102 80-81 0.790530280521395 1102 82-83 0.7471551416515254 1102 84-85 0.7462483517957139 1102 86-87 0.7841345025708115 1102 88-89 0.848175782403807 1102 90-91 0.8623532703225436 1102 92-93 0.8425242706850646 1102 94-95 0.8509512333983231 1102 96-97 0.8215024152844848 1102 98-99 0.7422718649024631 1102 100-101 0.7041712237221454 1102 102-103 0.6281818189463557 1102 104-105 0.8294627974064781 1102 106-107 0.8137180998341016 1102 108-109 0.8542601630899078 1102 110-111 0.8539422817333531 1102 112-113 0.7923843596163174 1102 114-115 0.8504512285933714 1102 116-117 0.8404946230808825 1102 118-119 0.7636450412363303 1102 120-121 0.8699541385979153 1102 122-123 0.9559499937867422 1102 124-125 1.0748861661140232 1103 1 0.1700779943957329 1103 2 0.3996280354555104 1103 3 0.25554232129364607 1103 4 0.41349630219861666 1103 5 0.05478416289194854 1103 6 0.05817327670632011 1103 7 0.12641123077251848 1103 8 0.11321848215725794 1103 9 0.20979603429017857 1103 10-11 0.08977607628484208 1103 12-13 0.12421756964195652 1103 14-15 0.2868901975161151 1103 16-17 0.19371654195448684 1103 18-19 0.04837264966477761 1103 20-21 0.07774930161127003 1103 22-23 0.1361099675819517 1103 24-25 0.035886950767007875 1103 26-27 -0.008078422163251275 1103 28-29 -0.0641000342338458 1103 30-31 0.030026863963932726 1103 32-33 0.030305368894680385 1103 34-35 0.04963271352815468 1103 36-37 -0.00248725480561518 1103 38-39 -0.09689116194248015 1103 40-41 -0.1538349411708282 1103 42-43 -0.3160708692584677 1103 44-45 -0.21050897341517327 1103 46-47 -0.29602000459897937 1103 48-49 -0.3188885452128929 1103 50-51 -0.30781469027572683 1103 52-53 -0.3275011149807554 1103 54-55 -0.3445021552309697 1103 56-57 -0.30661022722162556 1103 58-59 -0.43759000330895503 1103 60-61 -0.4427957342624964 1103 62-63 -0.34058464356736806 1103 64-65 -0.39778200356759186 1103 66-67 -0.461245381364499 1103 68-69 -0.4579706149171301 1103 70-71 -0.5079933960368379 1103 72-73 -0.43559347175912677 1103 74-75 -0.3498197514500063 1103 76-77 -0.3686670894328472 1103 78-79 -0.3972526552534106 1103 80-81 -0.3851218589128109 1103 82-83 -0.4031485743383243 1103 84-85 -0.3082577711497194 1103 86-87 -0.46094084573207184 1103 88-89 -0.45226407197111484 1103 90-91 -0.39627282372078554 1103 92-93 -0.4758161135887349 1103 94-95 -0.3541093691469861 1103 96-97 -0.3570219855418557 1103 98-99 -0.35586739409642476 1103 100-101 -0.366948524206844 1103 102-103 -0.41653059189659913 1103 104-105 -0.35860790078531934 1103 106-107 -0.2561068398392976 1103 108-109 -0.27059533304027283 1103 110-111 -0.3023118457526195 1103 112-113 -0.26984212118164663 1103 114-115 -0.25768856890429603 1103 116-117 -0.1428784903984912 1103 118-119 -0.0962845949233575 1103 120-121 -0.16075984916235697 1103 122-123 -0.18758270286778256 1103 124-125 -0.17202315899698206 1104 1 0.2029346656242197 1104 2 0.5962606593242405 1104 3 0.10202929282371542 1104 4 0.42501160834648744 1104 5 0.18700355105510624 1104 6 0.16802757716733652 1104 7 0.14405602367190795 1104 8 0.17998858746381075 1104 9 0.30049775366693865 1104 10-11 0.17944686187919956 1104 12-13 0.2275115492666302 1104 14-15 0.3131583588247935 1104 16-17 0.3265585810819829 1104 18-19 0.29104814244273314 1104 20-21 0.1965462282407202 1104 22-23 0.21413512168445692 1104 24-25 0.1794812241088124 1104 26-27 0.26078931806608807 1104 28-29 0.33712987514415715 1104 30-31 0.30712162097796636 1104 32-33 0.3296381660672765 1104 34-35 0.48750882473527213 1104 36-37 0.468467238049179 1104 38-39 0.348715182620424 1104 40-41 0.4370983220263298 1104 42-43 0.43522261633611947 1104 44-45 0.37316186409954355 1104 46-47 0.41114104527262185 1104 48-49 0.3261887361427469 1104 50-51 0.41075703835491595 1104 52-53 0.5127892404455636 1104 54-55 0.45692633640658187 1104 56-57 0.5119085845993254 1104 58-59 0.6432805437664229 1104 60-61 0.5998630023998075 1104 62-63 0.5899356902018695 1104 64-65 0.6437350096587338 1104 66-67 0.6012373610163877 1104 68-69 0.6600153838928975 1104 70-71 0.6798578694432322 1104 72-73 0.5133895316657728 1104 74-75 0.6173303951463325 1104 76-77 0.6104559442474766 1104 78-79 0.4802613193208174 1104 80-81 0.43364449239449243 1104 82-83 0.4193977363924333 1104 84-85 0.44177127016184414 1104 86-87 0.504545227156239 1104 88-89 0.6029407735094381 1104 90-91 0.5675904757445664 1104 92-93 0.5303747335863136 1104 94-95 0.4657756298814739 1104 96-97 0.5240640047983369 1104 98-99 0.5055767051155122 1104 100-101 0.6120542797547301 1104 102-103 0.45500113472016324 1104 104-105 0.5485792141550796 1104 106-107 0.45698296428721363 1104 108-109 0.4238435245754921 1104 110-111 0.4637084037115926 1104 112-113 0.4630381239873351 1104 114-115 0.5294399284759557 1104 116-117 0.43667044755484596 1104 118-119 0.44922084583197375 1104 120-121 0.48934806913807805 1104 122-123 0.5669492495238693 1104 124-125 0.7038583398650964 1105 1 0.08434051507510532 1105 2 0.5422411798141162 1105 3 0.11575101682084465 1105 4 0.42988273539813804 1105 5 0.10514277652291071 1105 6 0.06281603100683952 1105 7 0.1698593522920504 1105 8 0.07790608818478262 1105 9 0.17386794282920448 1105 10-11 0.13864292599254213 1105 12-13 0.2037540590216267 1105 14-15 0.2652631495327711 1105 16-17 0.24790382383613974 1105 18-19 0.15534219637899582 1105 20-21 0.1322499501596468 1105 22-23 0.23380093517916833 1105 24-25 0.21840750695305644 1105 26-27 0.2511135384033665 1105 28-29 0.35743356837410545 1105 30-31 0.3079048198380008 1105 32-33 0.36605978695404673 1105 34-35 0.3544898455327683 1105 36-37 0.4199362732051597 1105 38-39 0.2587517735512108 1105 40-41 0.3882683471516657 1105 42-43 0.40475420643972626 1105 44-45 0.5187483860818389 1105 46-47 0.5196595698999005 1105 48-49 0.5602890995530103 1105 50-51 0.5796927075900982 1105 52-53 0.5157784660883067 1105 54-55 0.6508239077903823 1105 56-57 0.6814740116926075 1105 58-59 0.6439321682168213 1105 60-61 0.6176124277457617 1105 62-63 0.6428331011473674 1105 64-65 0.5986695873136156 1105 66-67 0.6645351480172792 1105 68-69 0.6290001842371709 1105 70-71 0.5669892674207091 1105 72-73 0.6176815563822728 1105 74-75 0.5180763083427067 1105 76-77 0.3792788318471949 1105 78-79 0.4241210302619649 1105 80-81 0.36426629331204197 1105 82-83 0.4168632109174766 1105 84-85 0.44087399016599704 1105 86-87 0.3374043678166565 1105 88-89 0.4160811210458135 1105 90-91 0.4594927893050027 1105 92-93 0.5461624132234917 1105 94-95 0.5165249917518331 1105 96-97 0.6338864030861942 1105 98-99 0.5962392203783935 1105 100-101 0.5729426931597743 1105 102-103 0.5031160095507907 1105 104-105 0.6317644794205854 1105 106-107 0.5797653794485882 1105 108-109 0.5416096185123713 1105 110-111 0.5947259024833187 1105 112-113 0.6041987227371166 1105 114-115 0.5777142420024042 1105 116-117 0.6122107023268875 1105 118-119 0.6365270765862476 1105 120-121 0.6526345354917638 1105 122-123 0.720536445247113 1105 124-125 0.8711281496232459 1106 1 0.27676288847743535 1106 2 0.549835728819577 1106 3 0.382819763855089 1106 4 0.5018173179218408 1106 5 0.23737234333401602 1106 6 0.1929033298491376 1106 7 0.15637016457257857 1106 8 0.07841202859316354 1106 9 0.24689060648546501 1106 10-11 0.16774785927026414 1106 12-13 0.20856890462444966 1106 14-15 0.34646707988568437 1106 16-17 0.22136879568903112 1106 18-19 0.17580507371281584 1106 20-21 0.12176300540140517 1106 22-23 0.16104212748649616 1106 24-25 0.1738642165145734 1106 26-27 0.20764892775976307 1106 28-29 0.2979891577414904 1106 30-31 0.23361594444086364 1106 32-33 0.2516211383518012 1106 34-35 0.24675138183999223 1106 36-37 0.2374727615284442 1106 38-39 0.20641007352216434 1106 40-41 0.2539929997846073 1106 42-43 0.2364006181718068 1106 44-45 0.2799294217035069 1106 46-47 0.36061565625168157 1106 48-49 0.26197661492296476 1106 50-51 0.3938038927573544 1106 52-53 0.28908883541738106 1106 54-55 0.3433604579645788 1106 56-57 0.3598875260660179 1106 58-59 0.27050522230073426 1106 60-61 0.26660086855370224 1106 62-63 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-0.9803553206641809 2216 84-85 -0.8937522578937873 2216 86-87 -1.013677655164198 2216 88-89 -0.9657728606861475 2216 90-91 -0.976269265643662 2216 92-93 -0.9808282846815395 2216 94-95 -0.9532419390442435 2216 96-97 -0.9828778309511179 2216 98-99 -0.9305749990784413 2216 100-101 -1.0510363580080515 2216 102-103 -1.0228139268245293 2216 104-105 -1.0699253857582853 2216 106-107 -1.0822054898422309 2216 108-109 -1.1103423171620754 2216 110-111 -1.0744599940314004 2216 112-113 -1.2065048212022376 2216 114-115 -1.1531933110230668 2216 116-117 -1.1830476159007333 2216 118-119 -1.2532751583032038 2216 120-121 -1.3342726544923273 2216 122-123 -1.4564548409194167 2216 124-125 -1.8238941917079252 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27.0 3 9.0 4 26.0 5 127.0 6 362.0 7 673.0 8 1232.0 9 2332.0 10 4561.0 11 6268.0 12 6300.0 13 8161.0 14 11685.0 15 11889.0 16 6301.0 17 5169.0 18 5352.0 19 5060.0 20 4955.0 21 4836.0 22 5210.0 23 5520.0 24 6427.0 25 7690.0 26 9522.0 27 11968.0 28 15231.0 29 19339.0 30 24904.0 31 32140.0 32 41837.0 33 56167.0 34 78896.0 35 123097.0 36 224022.0 37 403781.0 38 197723.0 39 1404.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.388301265409666 18.54047679093296 11.983340503493599 24.087881440163777 2 14.10279958524663 22.211894534143088 37.59124574137165 26.094060139238636 3 21.16976830822716 26.617631919442985 29.436697849724375 22.77590192260548 4 13.382950563730049 17.80013820144082 34.94037563240491 33.87653560242423 5 12.276005904297353 38.43614626837594 35.083465227080666 14.204382600246038 6 32.25652826650333 32.660404231476555 18.274406054954685 16.808661447065436 7 24.903218256810273 32.993261013343925 22.893964833436158 19.20955589640965 8 27.131920163116213 33.12153802057913 22.058016461228423 17.688525355076237 9 28.045486493512456 14.553811537968736 20.655930997042667 36.74477097147614 10-11 24.283070236897885 26.8295040297674 26.960299273738297 21.927126459596415 12-13 26.571856675497855 24.6449069318048 27.169239611625173 21.61399678107217 14-15 25.731492892820533 24.008949891274078 25.928577142078414 24.330980073826975 16-17 22.321413692619842 27.477707841351744 29.614918443231698 20.585960022796716 18-19 23.261465127836335 25.677138919110682 31.16031441198101 19.90108154107197 20-21 21.83502444082358 25.666753073618725 31.31554584505999 21.182676640497704 22-23 20.926112591958397 26.42232316177641 28.64635910303858 24.00520514322661 24-25 21.761986901228926 27.291303603976587 27.828185835759513 23.118523659034977 26-27 22.85271177741421 24.84581948047812 28.70449850874276 23.596970233364907 28-29 22.31873055843579 24.403680936157606 30.732335950229594 22.54525255517701 30-31 23.630816995666578 25.593855146226158 29.665391056011575 21.109936802095685 32-33 20.481586842867333 25.93313746155208 30.81132985188153 22.77394584369906 34-35 19.676981666446945 26.74125797473267 31.36382556091607 22.217934797904313 36-37 23.270648932049475 25.59418842944357 29.231011929317297 21.904150709189654 38-39 23.995910244607664 24.645145952127198 29.228641915326808 22.130301887938334 40-41 21.92982163786543 26.122452002569986 29.271424101199635 22.67630225836495 42-43 23.745762674205288 25.571784390939733 28.767526068302324 21.91492686655266 44-45 24.785347092252056 24.31089991653107 28.529154504915187 22.37459848630169 46-47 22.44047746894356 25.708319415673053 31.14157648886871 20.70962662651468 48-49 23.818529510007014 25.56663701680414 29.98526888179037 20.629564591398477 50-51 21.565683086172967 26.299600874831413 29.837957699694044 22.296758339301572 52-53 20.99669457111264 27.113441460284122 27.26382625427436 24.626037714328884 54-55 21.84849241188177 27.555152817761474 26.377737273580344 24.21861749677641 56-57 22.978729865064736 25.482427457204583 27.15317622609341 24.385666451637274 58-59 22.791757980096325 24.697915794884175 28.984197191089045 23.526129033930452 60-61 23.787719328130656 26.126886105274536 28.102292766346988 21.983101800247816 62-63 20.835866902976814 26.340631742041754 29.222087345384363 23.601414009597075 64-65 19.808873184254516 26.83437231290406 30.009968871347493 23.34678563149393 66-67 23.009799267221297 26.163880542407327 27.920653412857177 22.905666777514195 68-69 23.810049303697294 25.046233788548832 27.945094182134095 23.198622725619778 70-71 21.85808356224953 26.355814644168323 27.993161028378694 23.792940765203454 72-73 23.573936659894844 25.95265304550501 27.549375908659663 22.924034385940484 74-75 24.45817406715879 24.658958689915515 27.509233796695753 23.373633446229938 76-77 22.157482985891754 25.863444237644266 30.271966511702313 21.70710626476167 78-79 23.19580833400607 25.657141926065936 29.45908874443324 21.687960995494752 80-81 21.08442212022933 26.222242136923114 29.366991482021593 23.326344260825966 82-83 20.622195329146802 26.876625218578244 26.9928299670494 25.50834948522556 84-85 21.382007001910083 27.14414054775467 26.230055776798007 25.24379667353724 86-87 22.39544720312427 25.039271872451774 27.087852715480558 25.477428208943397 88-89 22.258245611956127 24.492761458832486 28.605846676388662 24.643146252822724 90-91 23.051348574992055 25.801231370393936 27.716424863520523 23.430995191093487 92-93 20.484697486229848 25.871443034862164 28.631768704409634 25.012090774498354 94-95 19.535251454885135 26.369499164008097 29.428951797614044 24.666297583492724 96-97 22.52569130439613 25.542359637525784 27.6259938600097 24.305955198068386 98-99 23.164144348726474 24.66520312232668 27.44330802497802 24.727344503968826 100-101 21.369083019368198 25.780975157068976 27.67276476203949 25.17717706152334 102-103 22.765467193226225 25.13646652070736 27.540277065921664 24.557789220144755 104-105 23.548007858336593 23.961296507010868 27.57618319524944 24.914512439403097 106-107 21.450645976839503 25.173133348790454 29.987792330353763 23.38842834401628 108-109 22.4564712296579 25.157960665673784 28.84530836729471 23.54025973737361 110-111 20.44207305216631 25.645370433128477 29.030419328279187 24.882137186426025 112-113 19.969859303912145 26.17792418085306 26.939848978001514 26.91236753723328 114-115 20.515123090442174 26.749257960164723 26.18878263975022 26.546836309642885 116-117 21.37474317663002 24.82667895615424 26.842589594587686 26.955988272628055 118-119 21.098342973639294 24.457436468803337 28.183375213351034 26.260845344206334 120-121 21.91971585553521 25.53737266368769 27.36849707503792 25.17441440573919 122-123 19.72610704267079 25.721237258478148 28.015961041726463 26.536694657124606 124-125 18.85596735250115 26.32286065561629 28.68062036172955 26.140551630153013 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 584.0 1 428.0 2 617.0 3 859.5 4 510.0 5 347.0 6 690.0 7 1472.5 8 2241.5 9 2389.0 10 1904.5 11 1410.5 12 1335.5 13 1402.0 14 1567.5 15 1839.5 16 2003.0 17 2215.5 18 2474.5 19 2643.5 20 2884.0 21 3221.0 22 3702.5 23 4329.0 24 4959.5 25 5606.5 26 6728.5 27 8199.0 28 9288.0 29 10498.5 30 12519.0 31 14595.5 32 16639.0 33 18664.5 34 21002.0 35 23322.0 36 25449.0 37 27069.5 38 27641.0 39 28490.0 40 29261.0 41 30879.5 42 33037.0 43 34941.5 44 37379.5 45 39315.0 46 41597.0 47 46022.0 48 51968.5 49 71494.0 50 139952.0 51 137897.0 52 63674.5 53 40209.0 54 34673.0 55 30958.0 56 27476.5 57 24166.0 58 22254.0 59 20493.5 60 17947.0 61 14905.5 62 12470.0 63 10204.5 64 7925.5 65 6181.0 66 4516.0 67 3534.0 68 2700.0 69 1834.5 70 1487.5 71 1265.5 72 1086.0 73 977.0 74 742.5 75 575.0 76 379.5 77 170.0 78 84.0 79 55.0 80 31.5 81 16.5 82 9.0 83 3.5 84 0.5 85 1.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1498293219612162 2 2.2218881160832854E-4 3 2.9625174881110474E-4 4 0.0 5 0.0 6 0.002666265739299942 7 0.0 8 0.0 9 0.0 10-11 7.406293720277618E-5 12-13 0.004740027980977676 14-15 0.0014071958068527473 16-17 6.295349662235975E-4 18-19 0.0 20-21 2.2218881160832854E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.2218881160832854E-4 30-31 0.0 32-33 0.0 34-35 7.406293720277618E-5 36-37 0.0 38-39 0.0 40-41 3.703146860138809E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 7.7766084062915E-4 96-97 0.0028143916137054947 98-99 0.004147524483355466 100-101 0.0 102-103 0.0040734615461526895 104-105 0.022144818223630076 106-107 0.01718260143104407 108-109 0.019441521015728744 110-111 0.0164790035276177 112-113 0.014886650377758012 114-115 0.040364300775513014 116-117 0.03999398608949913 118-119 0.023329825218874497 120-121 0.01373867485111498 122-123 0.0029995489567124353 124-125 0.0017034475556638518 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1350203.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.24343946628086 #Duplication Level Percentage of deduplicated Percentage of total 1 87.62059818547355 56.290485955277894 2 8.541357707167506 10.974523936405344 3 1.9194520069143228 3.6993659644389485 4 0.6944415460128966 1.7845325369660006 5 0.34374306840708985 1.1041618503582258 6 0.18882022845744606 0.7278276550149154 7 0.12589970706182996 0.5661761146614416 8 0.09467447105433305 0.4865770920144964 9 0.06657354722491678 0.38492222860795783 >10 0.35545978171463294 4.203139160223149 >50 0.026718536850741482 1.191143523982079 >100 0.017348394244463224 2.3189916696780872 >500 9.211536405474984E-4 0.4235177792624218 >1k 0.0027634609216424947 5.278684570592644 >5k 7.676280337895818E-4 3.907627130088004 >10k+ 4.605768202737492E-4 6.658322832428404 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 35707 2.6445652986995287 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 19516 1.4454122824493798 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 12285 0.9098631835361052 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 9880 0.7317418195634285 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 9719 0.7198176866737817 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 8398 0.6219805466289143 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 6328 0.46867026661916766 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5294 0.3920891895514971 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 4978 0.3686853013954198 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4888 0.36201963704716994 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 4829 0.35764992375220617 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 4073 0.3016583432269074 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 3967 0.2938076718834131 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 3905 0.289215769776841 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 3638 0.26944096554369973 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 3402 0.2519621123638445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3349 0.2480367766920974 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 3304 0.2447039445179725 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 2782 0.20604309129812334 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 1971 0.14597804922667185 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1956 0.14486710516863022 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 1587 0.11753788134080578 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1488 0.11020565055773095 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.406293720277617E-5 0.0 0.0 0.0 0.0 3 7.406293720277617E-5 0.0 0.0 0.0 0.0 4 7.406293720277617E-5 0.0 0.0 0.0 0.0 5 7.406293720277617E-5 0.0 0.0 0.0 0.0 6 1.4812587440555234E-4 0.0 0.0 0.0 0.0 7 2.2218881160832854E-4 0.0 0.0 0.0 0.0 8 2.2218881160832854E-4 0.0 0.0 0.0 0.0 9 2.2218881160832854E-4 0.0 0.0 0.0 0.0 10-11 2.2218881160832854E-4 0.0 0.0 1.8515734300694043E-4 0.0 12-13 2.2218881160832854E-4 0.0 0.0 4.8140909181804516E-4 0.0 14-15 2.2218881160832854E-4 0.0 0.0 0.0011109440580416427 0.0 16-17 2.2218881160832854E-4 0.0 0.0 0.0019996993044749567 0.0 18-19 2.2218881160832854E-4 0.0 0.0 0.0029254860195096592 0.0 20-21 2.2218881160832854E-4 0.0 0.0 0.003962367140348526 0.0 22-23 2.2218881160832854E-4 0.0 0.0 0.006221286725033198 0.0 24-25 4.443776232166571E-4 0.0 0.0 0.008295048966710931 0.0 26-27 4.443776232166571E-4 0.0 0.0 0.011109440580416425 0.0 28-29 6.665664348249855E-4 0.0 0.0 0.015923531498596877 0.0 30-31 6.665664348249855E-4 0.0 0.0 0.027773601451041068 0.0 32-33 6.665664348249855E-4 0.0 0.0 0.051436709887328055 0.0 34-35 7.776608406291498E-4 0.0 0.0 0.09031975191878555 0.0 36-37 8.887552464333142E-4 0.0 0.0 0.1464224268498885 0.0 38-39 0.0010368811208388664 0.0 0.0 0.23215027666210192 0.0 40-41 0.0010739125894402546 0.0 0.0 0.34628126289158 0.0 42-43 0.001259069932447195 0.0 0.0 0.4388599343950502 0.0 44-45 0.0014071958068527473 0.0 0.0 0.5486952702667673 0.0 46-47 0.0015923531498596877 0.0 0.0 0.6846748229710644 0.0 48-49 0.0016293846184610758 0.0 0.0 0.8163957567862018 0.0 50-51 0.0016293846184610758 0.0 0.0 0.9451171416446268 0.0 52-53 0.0016293846184610758 0.0 0.0 1.091317379682907 0.0 54-55 0.0016293846184610758 0.0 0.0 1.2545520932778256 0.0 56-57 0.0017775104928666283 0.0 0.0 1.4478563593770715 0.0 58-59 0.0017775104928666283 0.0 0.0 1.6615649646756823 0.0 60-61 0.0017775104928666283 0.0 0.0 1.8629791223986318 0.0 62-63 0.0017775104928666283 0.0 0.0 2.057246206681514 0.0 64-65 0.0017775104928666283 0.0 0.0 2.288766948377392 0.0 66-67 0.0019256363672721807 0.0 0.0 2.5608371481917906 0.0 68-69 0.0019996993044749567 0.0 0.0 2.87908558935212 0.0 70-71 0.0019996993044749567 0.0 0.0 3.2794698278703276 0.0 72-73 0.0020367307730763447 0.0 0.0 3.6266028145397398 0.0 74-75 0.002110793710279121 0.0 0.0 3.9601082207638405 0.0 76-77 0.0021478251788805093 0.0 0.0 4.34771660261457 0.0 78-79 0.0021848566474818973 0.0 0.0 4.763913278225571 0.0 80-81 0.0028884545509082708 0.0 0.0 5.19181189791461 0.0 82-83 0.0028884545509082708 0.0 0.0 5.680664314921534 0.0 84-85 0.0029995489567124353 0.0 0.0 6.156740875260979 0.0 86-87 0.0031106433625165994 0.0 0.0 6.6561842922879 0.0 88-89 0.0031106433625165994 0.0 0.0 7.231394094073261 0.0 90-91 0.003332832174124928 0.0 0.0 7.796198053181632 0.0 92-93 0.003406895111327704 0.0 0.0 8.363779372435108 0.0 94-95 0.003406895111327704 0.0 0.0 8.969762324628222 0.0 96-97 0.003406895111327704 0.0 0.0 9.578411542560637 0.0 98-99 0.00348095804853048 0.0 0.0 10.268233739667295 0.0 100-101 0.0038512727345443613 0.0 0.0 11.158211024564455 0.0 102-103 0.0041475244833554656 0.0 0.0 11.952461963127025 0.0 104-105 0.004480807700767959 0.0 0.0 12.692202579908354 0.0 106-107 0.004591902106572123 0.0 0.0 13.467123091861001 0.0 108-109 0.004591902106572123 0.0 0.0 14.216269701667084 0.0 110-111 0.004591902106572123 0.0 0.0 15.010557671698255 0.0 112-113 0.004591902106572123 0.0 0.0 15.911903617456042 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1070 0.0 37.258274 5 AAAGTAC 1230 0.0 30.959253 3 GTACGGG 145 4.021009E-6 28.76666 1 AAGTACT 1425 0.0 27.976387 4 GTTATCA 800 0.0 23.835236 1 CATGGGG 3675 0.0 20.886423 4 CGTGTAC 175 4.784112E-4 20.399937 3 TTATCAA 990 0.0 19.833273 2 GAGTACT 7805 0.0 19.743969 12-13 ATAGCCC 310 1.9522486E-7 19.194199 5 ATGGGGA 2085 0.0 19.120554 5 AAAAGTA 1935 0.0 19.06454 2 AAAAAGT 1910 0.0 19.03075 1 AGTACTT 8010 0.0 18.830118 12-13 GCACCGT 380 4.26553E-9 18.790806 6 ACCGTGC 190 8.322894E-4 18.789415 8 TAGTACA 355 4.0059604E-8 18.437244 4 CGTACAG 165 0.008158591 18.030914 5 GTGAGCG 165 0.008158591 18.030914 9 GTGCTAT 235 1.6743861E-4 17.749643 1 >>END_MODULE