##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364166_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1502201 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.436289151718046 31.0 31.0 34.0 26.0 34.0 2 30.4809276521584 31.0 31.0 34.0 26.0 34.0 3 30.660897576289724 33.0 31.0 34.0 26.0 34.0 4 33.912045059216446 37.0 35.0 37.0 28.0 37.0 5 34.04849018207284 37.0 35.0 37.0 30.0 37.0 6 33.889115371378395 37.0 35.0 37.0 29.0 37.0 7 33.83692595065507 37.0 35.0 37.0 29.0 37.0 8 33.71485040949913 37.0 35.0 37.0 28.0 37.0 9 35.05621085327463 39.0 35.0 39.0 27.0 39.0 10-11 35.05848618127667 39.0 35.0 39.0 27.0 39.0 12-13 34.952244739552164 39.0 35.0 39.0 26.5 39.0 14-15 36.13466074113917 40.0 37.0 41.0 27.0 41.0 16-17 36.18521356329812 40.0 37.0 41.0 27.0 41.0 18-19 36.1123518091121 40.0 37.0 41.0 27.0 41.0 20-21 36.13579407815598 40.0 37.0 41.0 27.0 41.0 22-23 36.16381596071365 40.0 37.0 41.0 27.0 41.0 24-25 36.16322482810223 40.0 36.5 41.0 27.0 41.0 26-27 35.970614784572774 40.0 36.0 41.0 27.0 41.0 28-29 35.85182874994757 40.0 36.0 41.0 26.5 41.0 30-31 35.8848473007274 40.0 36.0 41.0 27.0 41.0 32-33 35.53501894886237 39.5 35.0 41.0 26.0 41.0 34-35 35.5690097397086 40.0 35.0 41.0 26.0 41.0 36-37 35.57993570767161 40.0 35.0 41.0 25.5 41.0 38-39 35.43456534777969 40.0 35.0 41.0 25.5 41.0 40-41 35.05112997528293 39.0 35.0 41.0 25.0 41.0 42-43 34.80529136913103 39.0 34.0 41.0 24.0 41.0 44-45 34.56887893164763 39.0 34.0 41.0 23.5 41.0 46-47 34.28963301182731 38.0 33.0 40.0 23.0 41.0 48-49 33.97879245187561 38.0 33.0 40.0 20.5 41.0 50-51 33.85994084679747 38.0 33.0 40.0 20.0 41.0 52-53 33.717548450573524 38.0 33.0 40.0 18.5 41.0 54-55 33.396540809119415 38.0 33.0 40.0 15.0 41.0 56-57 32.99466083433575 38.0 32.0 40.0 12.5 41.0 58-59 32.58010346152079 37.0 31.0 40.0 8.0 41.0 60-61 32.234128122668004 37.0 31.0 40.0 7.0 41.0 62-63 31.6028400992943 36.0 29.5 39.5 2.0 41.0 64-65 31.342305390556923 36.0 30.0 39.0 2.0 41.0 66-67 30.894323063291797 35.0 29.5 39.0 2.0 41.0 68-69 30.52441717186981 35.0 29.0 38.0 2.0 40.0 70-71 29.81048641293675 35.0 28.0 37.5 2.0 40.0 72-73 29.514314995130476 34.0 28.0 37.0 2.0 39.5 74-75 29.170278478046548 34.0 28.0 37.0 2.0 39.0 76-77 28.40700678537692 34.0 26.0 36.0 2.0 39.0 78-79 27.898310878504276 34.0 26.0 35.5 2.0 37.0 80-81 27.751038642631713 34.0 26.0 35.0 2.0 37.0 82-83 27.575334459236814 34.0 26.0 35.0 2.0 37.0 84-85 27.278285662171704 34.0 26.0 35.0 2.0 36.0 86-87 26.907416184651723 33.0 25.0 35.0 2.0 36.0 88-89 26.50915955987248 33.0 24.0 35.0 2.0 36.0 90-91 26.277022182783796 33.0 23.5 35.0 2.0 35.0 92-93 25.864054477396834 33.0 21.5 35.0 2.0 35.0 94-95 25.81838482333589 33.0 20.0 35.0 2.0 35.0 96-97 25.76515126803936 33.0 21.5 35.0 2.0 35.0 98-99 25.587669026981075 33.0 20.0 35.0 2.0 35.0 100-101 24.9586493418657 32.5 17.5 35.0 2.0 35.0 102-103 23.724140111742706 30.0 11.0 34.0 2.0 35.0 104-105 24.480132485599462 32.0 12.5 35.0 2.0 35.0 106-107 24.333970620442937 32.0 8.5 35.0 2.0 35.0 108-109 24.242464557006684 32.0 7.0 35.0 2.0 35.0 110-111 24.416227255873217 32.5 7.0 35.0 2.0 35.0 112-113 24.34526005507918 32.5 3.5 35.0 2.0 35.0 114-115 24.17451858972268 32.5 2.0 35.0 2.0 35.0 116-117 23.81980340846531 32.0 2.0 35.0 2.0 35.0 118-119 23.50098388963927 32.0 2.0 35.0 2.0 35.0 120-121 23.251194746908038 32.0 2.0 35.0 2.0 35.0 122-123 22.719404726797546 32.0 2.0 35.0 2.0 35.0 124-125 21.68812063099412 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3092639958298342 1101 2 0.41857386933303786 1101 3 0.40214621470779477 1101 4 0.4720487073264721 1101 5 0.37046198344567216 1101 6 0.3878364853907783 1101 7 0.40391422116717735 1101 8 0.30185662102236677 1101 9 0.43733294752496477 1101 10-11 0.4069731574667088 1101 12-13 0.4479167457797786 1101 14-15 0.46581241725468914 1101 16-17 0.4527527209780331 1101 18-19 0.3419487964789738 1101 20-21 0.30043867504453203 1101 22-23 0.33522406688808815 1101 24-25 0.43477522343037833 1101 26-27 0.5592837702836846 1101 28-29 0.5918765602499221 1101 30-31 0.38130022698511823 1101 32-33 0.5925398001151123 1101 34-35 0.4468329561448172 1101 36-37 0.47077762780325116 1101 38-39 0.3966999711611834 1101 40-41 0.3897150159206788 1101 42-43 0.3998096002859839 1101 44-45 0.5667561866770754 1101 46-47 0.5717838362671657 1101 48-49 0.7630788070762122 1101 50-51 0.7220188182969807 1101 52-53 0.7157234602023124 1101 54-55 0.8595345297369406 1101 56-57 0.7982461312123519 1101 58-59 0.787776155282593 1101 60-61 0.7767325917789165 1101 62-63 0.8118090961824116 1101 64-65 0.8043384939026197 1101 66-67 0.7409495136922217 1101 68-69 0.7880506979065025 1101 70-71 0.8154537757453753 1101 72-73 0.8662999902284518 1101 74-75 0.6718546565002157 1101 76-77 0.6650751439682239 1101 78-79 0.8966049494091308 1101 80-81 0.8413296908366803 1101 82-83 0.6978102885589053 1101 84-85 0.9249362315195349 1101 86-87 0.8613098089716793 1101 88-89 0.7784183213322606 1101 90-91 0.764082560269447 1101 92-93 1.080682972323384 1101 94-95 1.002498182498556 1101 96-97 0.7939290782175199 1101 98-99 0.7868379865712853 1101 100-101 1.0082068736388692 1101 102-103 1.0180787297963825 1101 104-105 0.9536658940627163 1101 106-107 0.7287671517737522 1101 108-109 0.9208803785385022 1101 110-111 0.8187982050185951 1101 112-113 0.770735521729236 1101 114-115 0.839782337935354 1101 116-117 0.9836952414186868 1101 118-119 1.0303526948086592 1101 120-121 1.063420052340014 1101 122-123 1.0444368344034167 1101 124-125 0.9639772489053797 1102 1 0.0034107151433424576 1102 2 0.1277275956801489 1102 3 0.05203156170539813 1102 4 0.07491303986500952 1102 5 0.09475638180383328 1102 6 0.13816608163952537 1102 7 0.17517193388544428 1102 8 0.09532430564608063 1102 9 0.16411135589886783 1102 10-11 0.17016385670889633 1102 12-13 0.34801317551351474 1102 14-15 0.25632452846394216 1102 16-17 0.22167295702539747 1102 18-19 0.25976600588633403 1102 20-21 0.32952305334464427 1102 22-23 0.2912476666436774 1102 24-25 0.34317693899130575 1102 26-27 0.5261165910962404 1102 28-29 0.5144826703168093 1102 30-31 0.3547181234802679 1102 32-33 0.47980958087134695 1102 34-35 0.36090754618354026 1102 36-37 0.24395978410991148 1102 38-39 0.28040998789836635 1102 40-41 0.32438252221372466 1102 42-43 0.48391081539195113 1102 44-45 0.48212176454148903 1102 46-47 0.4889331358958984 1102 48-49 0.43260730179271434 1102 50-51 0.37925449372853137 1102 52-53 0.3563796971263926 1102 54-55 0.40870850508280654 1102 56-57 0.4244256623720659 1102 58-59 0.3676675462617922 1102 60-61 0.43699295621107836 1102 62-63 0.41936685439658206 1102 64-65 0.4353347379997743 1102 66-67 0.3443597140734589 1102 68-69 0.4236210239494227 1102 70-71 0.33446242993000297 1102 72-73 0.2683345621352302 1102 74-75 0.49320913103911224 1102 76-77 0.17300724714098692 1102 78-79 0.24271920058637875 1102 80-81 0.37368803086710045 1102 82-83 0.46102817741606117 1102 84-85 0.40880448766479915 1102 86-87 0.3937219055691159 1102 88-89 0.4557574999485219 1102 90-91 0.3159188347857622 1102 92-93 0.3596631583584653 1102 94-95 0.3798885855192218 1102 96-97 0.4436286641051588 1102 98-99 0.3488801217572224 1102 100-101 0.1314331144815739 1102 102-103 0.02052039873230882 1102 104-105 0.09158926334999151 1102 106-107 0.023994066752862864 1102 108-109 0.03508236541442855 1102 110-111 0.2508997173423424 1102 112-113 0.24107559531969613 1102 114-115 0.30918903978109213 1102 116-117 0.17446256141098004 1102 118-119 0.3924060794383486 1102 120-121 0.32585629165511776 1102 122-123 0.4246138616279005 1102 124-125 0.23873889353254896 1103 1 0.44105661740960755 1103 2 0.4562652060421186 1103 3 0.4737973948031815 1103 4 0.36008483874434916 1103 5 0.4439593231270109 1103 6 0.41966392843798417 1103 7 0.45120281029666387 1103 8 0.32879993872058577 1103 9 0.5291548771731627 1103 10-11 0.5816688323502746 1103 12-13 0.501336193154799 1103 14-15 0.4650840682275046 1103 16-17 0.537256857063646 1103 18-19 0.5111641320190472 1103 20-21 0.5272616721365253 1103 22-23 0.5680495180644556 1103 24-25 0.5672846905168356 1103 26-27 0.7639073564388354 1103 28-29 0.730567355982636 1103 30-31 0.6885284796314437 1103 32-33 0.8515136860872516 1103 34-35 0.7969813619226969 1103 36-37 0.8216467135946388 1103 38-39 0.7386344487666321 1103 40-41 0.8760000213408077 1103 42-43 0.8967002297876405 1103 44-45 0.8893031904763475 1103 46-47 0.8547886801820184 1103 48-49 0.8869071579688281 1103 50-51 0.8515542626126873 1103 52-53 0.8195545470673622 1103 54-55 0.7900104645793391 1103 56-57 0.8793590087299137 1103 58-59 0.8141137245369023 1103 60-61 0.8168899309213558 1103 62-63 0.9513914665708931 1103 64-65 0.9510682813880322 1103 66-67 0.9733356547481833 1103 68-69 0.855287635192628 1103 70-71 0.7790453406594331 1103 72-73 0.8352657719185821 1103 74-75 0.9441573918508439 1103 76-77 0.6846394196298853 1103 78-79 0.8000633636643748 1103 80-81 0.8098785476354706 1103 82-83 0.8269614531089928 1103 84-85 0.8296376477998102 1103 86-87 0.7271520221246597 1103 88-89 0.6707252642768715 1103 90-91 0.5922431784333995 1103 92-93 0.5526703453960238 1103 94-95 0.7139423199521566 1103 96-97 0.6166264568657418 1103 98-99 0.6261310071470447 1103 100-101 0.45287929275668404 1103 102-103 0.3804596269051643 1103 104-105 0.46046043289590344 1103 106-107 0.3778872879091111 1103 108-109 0.5382677943088972 1103 110-111 0.49639877791464215 1103 112-113 0.3964725243056151 1103 114-115 0.3505885999959162 1103 116-117 0.3477412472098287 1103 118-119 0.48858071738457554 1103 120-121 0.463707122175709 1103 122-123 0.31023365528634983 1103 124-125 0.37064638900168134 1104 1 0.3189629883913554 1104 2 0.4498983102230447 1104 3 0.4470223148722354 1104 4 0.5060310883740584 1104 5 0.36680680568520785 1104 6 0.4478425243091593 1104 7 0.4709470312635986 1104 8 0.46530973983809076 1104 9 0.6433759462559792 1104 10-11 0.4641294797654183 1104 12-13 0.580475492622611 1104 14-15 0.6017853971752842 1104 16-17 0.472622598608325 1104 18-19 0.4943127474005209 1104 20-21 0.6014779402550587 1104 22-23 0.6141239636552314 1104 24-25 0.5503260503133589 1104 26-27 0.523818793970122 1104 28-29 0.6018703581175302 1104 30-31 0.65383946975372 1104 32-33 0.6031787013584022 1104 34-35 0.5251701726114817 1104 36-37 0.5271984669680805 1104 38-39 0.5802608520271946 1104 40-41 0.6529947198504686 1104 42-43 0.67191755003239 1104 44-45 0.5852611871463225 1104 46-47 0.6250334272120597 1104 48-49 0.6899542380632084 1104 50-51 0.631873884255846 1104 52-53 0.7331979682140783 1104 54-55 0.7332819642167081 1104 56-57 0.6898950823583192 1104 58-59 0.6889045761462498 1104 60-61 0.6083242956107782 1104 62-63 0.5267170638823551 1104 64-65 0.502724103323331 1104 66-67 0.6130268608117007 1104 68-69 0.7247887435003371 1104 70-71 0.7218556412815111 1104 72-73 0.6298789878496116 1104 74-75 0.5575730225647568 1104 76-77 0.4847022407582138 1104 78-79 0.49273065683985706 1104 80-81 0.5402277922497056 1104 82-83 0.6321035922960974 1104 84-85 0.5705674004990762 1104 86-87 0.5133640613086996 1104 88-89 0.4867681460812179 1104 90-91 0.5466682374239724 1104 92-93 0.328157847566672 1104 94-95 0.4653029336583998 1104 96-97 0.31930069611720313 1104 98-99 0.4694326404963789 1104 100-101 0.3684773192539197 1104 102-103 0.2513172629920746 1104 104-105 0.3708933368664056 1104 106-107 0.3873324190162961 1104 108-109 0.32150936453066237 1104 110-111 0.30203224982900423 1104 112-113 0.3842979939318525 1104 114-115 0.3517357240140129 1104 116-117 0.3123811238148342 1104 118-119 0.1701230526961801 1104 120-121 0.1790906375030481 1104 122-123 0.29113807523793156 1104 124-125 0.36069766738610554 1105 1 0.14392503452843997 1105 2 0.20787622195181044 1105 3 0.1294738908101678 1105 4 0.13195762060902183 1105 5 0.043409101820834906 1105 6 0.056863363367234854 1105 7 0.18252632121941303 1105 8 0.09202321789305756 1105 9 0.14694312215726768 1105 10-11 0.10144343762737407 1105 12-13 0.03848088314300924 1105 14-15 0.09737388180761997 1105 16-17 0.09238511168998542 1105 18-19 0.04641799312133088 1105 20-21 0.02609747592194367 1105 22-23 0.14149059894540272 1105 24-25 0.13651318283324088 1105 26-27 0.3130396045749819 1105 28-29 0.36937785631971565 1105 30-31 0.21279385301075138 1105 32-33 0.08933669856374848 1105 34-35 0.2162372267096231 1105 36-37 0.20506166935815173 1105 38-39 0.25766529501650126 1105 40-41 0.32803959213255496 1105 42-43 0.2667489062569217 1105 44-45 0.1859015794109382 1105 46-47 0.38249655008903005 1105 48-49 0.3660437916400383 1105 50-51 0.2633746160491981 1105 52-53 0.33919857625541994 1105 54-55 0.3277147805200187 1105 56-57 0.43979257282356343 1105 58-59 0.4131521737008299 1105 60-61 0.48968753600342296 1105 62-63 0.48630131561147394 1105 64-65 0.5589518498870234 1105 66-67 0.562473387278061 1105 68-69 0.5230553384493071 1105 70-71 0.5525102796430765 1105 72-73 0.29508041128999096 1105 74-75 0.26296519948919794 1105 76-77 0.30795660667047997 1105 78-79 0.24407770228510017 1105 80-81 0.21010148725071076 1105 82-83 0.26957024259242246 1105 84-85 0.3635977265612489 1105 86-87 0.448165268816453 1105 88-89 0.2144294475625408 1105 90-91 0.27506425553269054 1105 92-93 0.17632758464726095 1105 94-95 0.14984747755917738 1105 96-97 0.13507975883705825 1105 98-99 0.09111691502704744 1105 100-101 0.14492078407619502 1105 102-103 -0.002475394908664441 1105 104-105 -0.021148434794579174 1105 106-107 0.11797097936147338 1105 108-109 0.19908783986698353 1105 110-111 0.15230167840655895 1105 112-113 0.3270515832272096 1105 114-115 0.43211411282137746 1105 116-117 0.3447931380882139 1105 118-119 0.21107376042122894 1105 120-121 0.30144668079091375 1105 122-123 0.18992803398076674 1105 124-125 0.07700165298020067 1106 1 3.535121830822163E-4 1106 2 0.034221538299775034 1106 3 0.0433383338059663 1106 4 -0.004422168975217744 1106 5 -0.07566446673631333 1106 6 0.033917044737656 1106 7 0.0500895636502392 1106 8 0.05317292182613187 1106 9 0.1410065049256346 1106 10-11 0.11357867321335391 1106 12-13 0.20904191638942393 1106 14-15 0.16083049713791553 1106 16-17 0.0811414155614969 1106 18-19 0.10304079299095292 1106 20-21 0.07772358051415296 1106 22-23 0.07227590062582578 1106 24-25 0.16452381906024272 1106 26-27 0.19610816398756015 1106 28-29 0.22169608526003515 1106 30-31 0.11845336019965913 1106 32-33 -0.11810170004917353 1106 34-35 0.07913481199862105 1106 36-37 0.21358575988038808 1106 38-39 0.0785728148448328 1106 40-41 0.11245341130537412 1106 42-43 0.24167262601644524 1106 44-45 0.2024605596550373 1106 46-47 0.18430741357229152 1106 48-49 0.3154308900936087 1106 50-51 0.38231803286073074 1106 52-53 0.3000146956057037 1106 54-55 0.2980710228240824 1106 56-57 0.24492824753021125 1106 58-59 0.09384353977355175 1106 60-61 0.21178639700588775 1106 62-63 0.2502306647169341 1106 64-65 0.3409241920752031 1106 66-67 0.289013252191868 1106 68-69 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-2.3597922455616107 2216 98-99 -2.2057588297755046 2216 100-101 -2.3368827673693744 2216 102-103 -2.2164848330466427 2216 104-105 -2.3123683508825614 2216 106-107 -2.3585827016851972 2216 108-109 -2.3075413284737145 2216 110-111 -2.4002962016354665 2216 112-113 -2.4752644395197265 2216 114-115 -2.624185234030982 2216 116-117 -2.5357323692767437 2216 118-119 -2.447397175320546 2216 120-121 -2.462542773721591 2216 122-123 -2.3923370178622925 2216 124-125 -2.4450062639926813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47442.0 3 8339.0 4 6394.0 5 4848.0 6 6166.0 7 5580.0 8 5625.0 9 6359.0 10 7677.0 11 9193.0 12 10686.0 13 11695.0 14 12398.0 15 12728.0 16 12850.0 17 13399.0 18 13968.0 19 14995.0 20 15857.0 21 17168.0 22 18425.0 23 21600.0 24 24625.0 25 24513.0 26 27969.0 27 30819.0 28 35110.0 29 42577.0 30 50497.0 31 60343.0 32 72305.0 33 85828.0 34 98364.0 35 117122.0 36 158702.0 37 259780.0 38 129410.0 39 845.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.740698004983365 17.34236358467533 9.640361878961187 23.276576531380123 2 12.18480734799088 24.517625908182218 36.10309820306497 27.19446854076194 3 22.879816183084003 32.25549534873923 26.062440776754865 18.8022476914219 4 13.557771524560522 19.774268962049153 31.59371890480725 35.074240608583075 5 10.74759136495895 43.23906994286952 33.12082998684588 12.892508705325648 6 31.22438156281746 29.070978243931993 21.199910918562043 18.5047292746885 7 24.78757699017642 34.33225252309789 22.22365616994643 18.65651431677926 8 29.730722178783243 32.88448122600033 22.308342134464336 15.076454460752084 9 32.05674252270898 16.120016616573896 18.41362559059411 33.40961527012301 10-11 23.1433290563584 31.274476092869673 23.87037019693262 21.711824653839308 12-13 27.306181973869936 28.316096144712105 23.249968223811404 21.12775365760655 14-15 28.49183046970619 26.407948153746347 22.24071857088397 22.859502805663496 16-17 23.02275124383766 29.895376515462114 29.288155000331066 17.793717240369162 18-19 25.331950394769443 28.98623849069776 28.356058531470378 17.32575258306241 20-21 21.41205921428612 29.81099354707538 28.62139754623671 20.155549692401785 22-23 19.75026087654519 31.87603681703858 22.261632418258685 26.112069888157542 24-25 20.81035076630851 33.50806438128488 20.949399894994166 24.732184957412443 26-27 23.998937801168154 27.881615665961213 22.428482179407254 25.690964353463386 28-29 23.23648538150749 27.822675175935927 25.970026262887725 22.970813179668852 30-31 25.9797471538249 30.26842580210997 23.951786773839874 19.800040270225253 32-33 19.049632234278356 30.789404413857596 26.98762563921265 23.173337712651403 34-35 16.836673434830143 32.431063897104295 28.188492293758582 22.543770374306977 36-37 24.342109222004332 29.386668413954858 24.907983212661243 21.363239151379567 38-39 27.098735253338646 27.287175722157407 23.850367774708207 21.763721249795736 40-41 21.612838874834136 31.79324353810873 24.10396633631407 22.48995125074307 42-43 25.460190662949252 29.56144580890061 23.430349074204962 21.54801445394518 44-45 27.578274833010237 27.296763235073385 23.4388074151111 21.686154516805285 46-47 22.70415276160954 29.197243305011707 29.934726747657564 18.16387718572119 48-49 24.896855811428253 28.74611234751526 28.25346741994269 18.103564421113795 50-51 20.19172336427105 30.196129030532802 28.648496691739073 20.963650913457073 52-53 18.85681816282704 31.952604179723075 22.770750294552656 26.41982736289723 54-55 20.260375076752453 33.174942784533926 21.326269043549935 25.238413095163686 56-57 23.52851731357599 27.838138157707853 22.672220913309303 25.96112361540685 58-59 23.037106254871055 27.60176247023893 25.9276229511484 23.433508323741613 60-61 25.209495316082148 30.067119388013968 24.224246473650098 20.499138822253784 62-63 18.85945487939595 30.65136046179326 26.87836647653682 23.61081818227397 64-65 16.590782059407953 31.92615859414906 28.170940273799687 23.3121190726433 66-67 23.61457204178587 29.336948634050746 24.998454071207654 22.05002525295573 68-69 26.338974881278805 27.314482565440805 23.896595650661016 22.44994690261937 70-71 21.108469790719838 31.181249663613308 24.266610148130493 23.443670397536362 72-73 24.88579643634737 29.169487281575762 23.683905262105746 22.260811019971126 74-75 26.84179523216676 26.998052963146712 23.619322201167954 22.540829603518574 76-77 22.137378552338866 28.77465097116344 29.722765889524204 19.36520458697349 78-79 24.30669408838658 28.635207897348376 27.946355842607883 19.11174217165716 80-81 20.010008346974747 29.89246542624226 28.374800225839653 21.72272600094334 82-83 18.73052027495912 31.268777752967335 22.95321656974995 27.047485402323595 84-85 20.02781762043355 32.42955593758451 21.783784018673437 25.758842423308504 86-87 23.004720103227186 27.304827742975 23.10868271680049 26.58176943699732 88-89 22.77458587210443 26.895224865288732 26.091720316642093 24.238468945964744 90-91 24.62395088807456 29.315736185850373 24.548174497617907 21.51213842845716 92-93 19.12159663994769 29.87796276102484 26.659684386681477 24.340756212345998 94-95 17.090930849579124 30.77222644345148 28.10432783897539 24.03251486799401 96-97 23.49336371240448 28.413175863876987 25.399919997620646 22.693540426097886 98-99 25.646735037915107 26.79711726254867 24.222304332458258 23.333843367077968 100-101 21.168342149230256 29.658730744701355 24.568139093505525 24.60478801256286 102-103 24.309555397899974 28.349078850503762 24.248096442461726 23.093269309134538 104-105 25.94517696368936 26.33134454191326 24.17503903095019 23.548439463447192 106-107 21.542639201819586 28.021340314471963 29.375973871511768 21.060046612196683 108-109 23.237804399287 28.412738453416907 28.041470304729227 20.30798684256687 110-111 19.682423624308555 29.64246675914301 28.289941743913154 22.38516787263528 112-113 18.62865296288685 30.819837566267516 23.307252377109574 27.244257093736056 114-115 19.552386582089916 32.502547093239485 22.333287776926426 25.611778547744173 116-117 22.32061400212128 27.817135603270447 23.32399635874694 26.538254035861332 118-119 21.867661925724647 27.508351029403112 25.91506956293828 24.708917481933963 120-121 23.39750608567385 29.76264785404145 24.72914948340728 22.110696576877416 122-123 19.213342079769234 30.300819694318083 25.983431006694495 24.502407219218185 124-125 17.665940440547477 31.21905742087254 27.128154405474763 23.98684773310522 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8480.0 1 5421.0 2 4041.0 3 5929.5 4 5666.0 5 4483.5 6 3300.5 7 2638.5 8 2420.5 9 2202.0 10 1783.0 11 1504.5 12 1415.0 13 1345.5 14 1365.5 15 1426.5 16 1459.5 17 1562.5 18 1724.5 19 1895.5 20 2140.0 21 2502.0 22 2989.0 23 3602.5 24 4344.0 25 5409.0 26 6794.0 27 8257.5 28 9566.0 29 10924.5 30 12714.0 31 14535.0 32 16348.0 33 18276.5 34 20464.5 35 22250.5 36 23693.0 37 25287.5 38 25648.0 39 25160.5 40 25035.0 41 25635.0 42 26269.5 43 27034.0 44 28575.5 45 30027.5 46 33689.5 47 43368.5 48 57656.5 49 97196.0 50 224110.5 51 238462.0 52 108117.0 53 46458.5 54 29457.0 55 22243.0 56 18693.0 57 16266.5 58 14760.0 59 13480.5 60 11894.0 61 10477.5 62 8995.0 63 6663.5 64 4701.5 65 3710.5 66 2635.5 67 1887.0 68 1414.0 69 1029.0 70 919.5 71 858.5 72 854.5 73 865.0 74 615.5 75 457.0 76 343.0 77 197.0 78 124.0 79 73.0 80 46.5 81 31.0 82 23.5 83 21.0 84 17.5 85 12.5 86 12.0 87 12.0 88 7.0 89 6.5 90 9.0 91 6.5 92 3.5 93 3.5 94 3.0 95 1.0 96 1.0 97 1.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48102750564005753 2 0.46891194986556395 3 0.4526691168492099 4 0.4522031339348064 5 0.4561307042133509 6 0.46198877513728187 7 0.4472104598519106 8 0.47450374483840707 9 0.4864861626373567 10-11 0.49094628481807695 12-13 0.4906800088669892 14-15 0.4667151732690898 16-17 0.4679799840367567 18-19 0.45496574692734193 20-21 0.46515080205644915 22-23 0.48202604045663666 24-25 0.46904508784110777 26-27 0.4785644530924956 28-29 0.4743373223689773 30-31 0.4859536107351813 32-33 0.48565405029020753 34-35 0.48006225531736435 36-37 0.43602686990622425 38-39 0.39758327946792743 40-41 0.3907932427151892 42-43 0.39944721112554177 44-45 0.43439592970581165 46-47 0.442151216781243 48-49 0.4393886037887073 50-51 0.4260415217404329 52-53 0.4186190796038613 54-55 0.4207492872125634 56-57 0.40264252253859506 58-59 0.407335636176517 60-61 0.41835280365277344 62-63 0.418951924542721 64-65 0.4332975414075746 66-67 0.42181439101691454 68-69 0.42367832267452893 70-71 0.4344624986935836 72-73 0.4337968088158642 74-75 0.43902247435596164 76-77 0.42893727270851234 78-79 0.41981732138375627 80-81 0.429336686635144 82-83 0.427040056557012 84-85 0.42514284040551165 86-87 0.43076791987224083 88-89 0.42048301126147564 90-91 0.42287949482126563 92-93 0.43336411039534656 94-95 0.4296695315740037 96-97 0.39911436618668206 98-99 0.37065612391417657 100-101 0.3706228394202906 102-103 0.3506188585948219 104-105 0.3536144630445593 106-107 0.37258662455956293 108-109 0.38000906669613455 110-111 0.396917589590208 112-113 0.3926571743728036 114-115 0.39192491550731223 116-117 0.3636996646920086 118-119 0.4378242325760667 120-121 0.4447141228104628 122-123 0.39758327946792743 124-125 0.3914922170867946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1502201.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.22188958026204 #Duplication Level Percentage of deduplicated Percentage of total 1 85.98638260681696 44.90371377899359 2 9.642100998003492 10.070574672789453 3 2.2649291620707794 3.54836641826327 4 0.8086091241084288 1.6890838557113108 5 0.36514777368804885 0.9534353359007899 6 0.21288764255915504 0.6670436977635893 7 0.1356607903197169 0.4959123968713135 8 0.09547628957032081 0.3988761801179534 9 0.06761958140967457 0.3178100082455608 >10 0.3683767867442323 3.5503956782490222 >50 0.02841672523460729 1.03344192153568 >100 0.0174019810950739 1.8917831251215373 >500 0.002082288027902146 0.7717505212029718 >1k 0.0017848183096304108 1.8784233700455586 >5k 0.0017848183096304108 6.9333271462019 >10k+ 0.001338613732222808 20.89606189298654 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 89903 5.984751707660959 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 44804 2.9825569281341178 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 26543 1.7669406424306735 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 20811 1.3853672045218983 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 19993 1.330913772524449 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 19607 1.3052181432444794 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 17475 1.1632930613146975 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 15189 1.0111163552680367 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 14704 0.9788303961986445 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 9914 0.6599649447710393 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 9796 0.65210980421395 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 9132 0.6079079963333802 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 8680 0.5778188138604621 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 8353 0.5560507548590369 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 7902 0.5260281413738908 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 6800 0.4526691168492099 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 6424 0.4276391774469595 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 6119 0.407335636176517 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5542 0.3689253302321061 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5455 0.3631338282959471 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5147 0.34263058006218877 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4349 0.28950852782017855 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3880 0.2582876725551374 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3179 0.21162281212700565 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2138 0.14232449585641335 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 1931 0.12854471538762122 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1700 0.11316727921230248 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.9941392663165584E-4 0.0 12-13 0.0 0.0 0.0 0.0012315262737809386 0.0 14-15 0.0 0.0 0.0 0.002329914572017992 0.0 16-17 0.0 0.0 0.0 0.0041272772418604435 0.0 18-19 0.0 0.0 0.0 0.005924639911702894 0.0 20-21 0.0 0.0 0.0 0.008221269989834915 0.0 22-23 0.0 0.0 0.0 0.012514969701125216 0.0 24-25 0.0 0.0 0.0 0.017174798845161203 0.0 26-27 0.0 0.0 0.0 0.022167472928056896 0.0 28-29 0.0 0.0 0.0 0.03168683817944469 0.0 30-31 0.0 0.0 0.0 0.04876178354294798 0.0 32-33 0.0 0.0 0.0 0.07988278532633117 0.0 34-35 0.0 0.0 0.0 0.12678063721166474 0.0 36-37 0.0 0.0 0.0 0.1927505040936599 0.0 38-39 0.0 0.0 0.0 0.2950670382991357 0.0 40-41 0.0 0.0 0.0 0.44797600321128794 0.0 42-43 0.0 0.0 0.0 0.5669014998658635 0.0 44-45 0.0 0.0 0.0 0.6808010379436573 0.0 46-47 0.0 0.0 0.0 0.8359067794522836 0.0 48-49 0.0 0.0 0.0 0.9779649993576093 0.0 50-51 0.0 0.0 0.0 1.118725124001382 0.0 52-53 0.0 0.0 0.0 1.2994266413083202 0.0 54-55 0.0 0.0 0.0 1.4850875482042682 0.0 56-57 0.0 0.0 0.0 1.681366208649841 0.0 58-59 0.0 0.0 0.0 1.9199827453183695 0.0 60-61 0.0 0.0 0.0 2.141025069215105 0.0 62-63 0.0 0.0 0.0 2.3536464161586896 0.0 64-65 0.0 0.0 0.0 2.6066751386798437 0.0 66-67 0.0 0.0 0.0 2.881838049635169 0.0 68-69 0.0 0.0 0.0 3.2222052841131115 0.0 70-71 0.0 0.0 0.0 3.7340875155854643 0.0 72-73 0.0 0.0 0.0 4.128175923195364 0.0 74-75 0.0 0.0 0.0 4.485285258097951 0.0 76-77 0.0 0.0 0.0 4.907132933608752 0.0 78-79 0.0 0.0 0.0 5.308843490318539 0.0 80-81 0.0 0.0 0.0 5.738446452904771 0.0 82-83 0.0 0.0 0.0 6.298324924560694 0.0 84-85 0.0 0.0 0.0 6.8188611244434 0.0 86-87 0.0 0.0 0.0 7.323553905236383 0.0 88-89 0.0 0.0 0.0 7.917182853692681 0.0 90-91 0.0 0.0 0.0 8.472967332600632 0.0 92-93 0.0 0.0 0.0 9.024624534266719 0.0 94-95 0.0 0.0 0.0 9.646445449044435 0.0 96-97 0.0 0.0 0.0 10.257149342864238 0.0 98-99 0.0 0.0 0.0 10.969936779432313 0.0 100-101 0.0 0.0 0.0 12.024489399221544 0.0 102-103 0.0 0.0 0.0 12.868817155626976 0.0 104-105 0.0 0.0 0.0 13.602973237269847 0.0 106-107 0.0 0.0 0.0 14.400669417741035 0.0 108-109 0.0 0.0 0.0 15.10024291023638 0.0 110-111 0.0 0.0 0.0 15.835197819732514 0.0 112-113 0.0 0.0 0.0 16.761338862109664 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1545 0.0 48.158695 5 TATCACG 65 3.5122786E-5 45.7878 2 AAGTACT 1735 0.0 42.883396 4 AAAGTAC 2260 0.0 32.658173 3 AAAAAGT 2425 0.0 30.6846 1 GAACAAA 1725 0.0 29.67779 1 AAAAGTA 2545 0.0 29.001944 2 GTACAAA 1310 0.0 28.628029 1 ATCACGC 105 5.8936415E-4 28.343882 3 GTCGGGA 555 0.0 27.885183 2 CGGGAGT 575 0.0 25.879196 4 TACAACG 210 9.777068E-8 25.510347 2 TCACGCA 120 0.0012852522 24.800898 4 TCGGGAG 660 0.0 23.44812 3 AGACCGT 155 2.1037532E-4 23.036982 6 CGTGAAC 130 0.00204801 22.893135 3 ATAACGC 130 0.00204801 22.893135 3 GTACATA 1010 0.0 22.39672 1 GTCCTAC 215 3.409472E-6 22.15 1 ATGGGAG 2880 0.0 21.908192 5 >>END_MODULE