##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364166_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1502201 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.057124845476736 33.0 31.0 34.0 30.0 34.0 2 31.864812365322617 33.0 31.0 34.0 30.0 34.0 3 32.11790233131252 34.0 31.0 34.0 30.0 34.0 4 35.54499431167999 37.0 35.0 37.0 33.0 37.0 5 35.69669704653372 37.0 35.0 37.0 33.0 37.0 6 35.86328726981276 37.0 35.0 37.0 35.0 37.0 7 35.83304963849711 37.0 35.0 37.0 35.0 37.0 8 35.90349560411689 37.0 35.0 37.0 35.0 37.0 9 37.56580178018787 39.0 37.0 39.0 35.0 39.0 10-11 37.52030886678946 39.0 37.0 39.0 34.5 39.0 12-13 37.506745768375865 39.0 37.0 39.0 34.5 39.0 14-15 38.7729631387544 40.0 38.0 41.0 34.0 41.0 16-17 38.74052506954795 40.0 38.0 41.0 34.0 41.0 18-19 38.804979160578384 40.0 38.0 41.0 34.0 41.0 20-21 38.84434073735805 40.0 38.0 41.0 35.0 41.0 22-23 38.80262328410113 40.0 38.0 41.0 35.0 41.0 24-25 38.77072542223045 40.0 38.0 41.0 34.5 41.0 26-27 38.55055481922859 40.0 38.0 41.0 34.0 41.0 28-29 38.378565851041245 40.0 38.0 41.0 34.0 41.0 30-31 38.21140812714144 40.0 37.5 41.0 34.0 41.0 32-33 37.84466559401838 40.0 37.0 41.0 33.5 41.0 34-35 37.5985317544057 40.0 36.5 41.0 33.0 41.0 36-37 37.492437430144165 40.0 37.0 41.0 33.0 41.0 38-39 37.38244149750932 40.0 36.5 41.0 33.0 41.0 40-41 37.104161493701575 40.0 36.0 41.0 32.0 41.0 42-43 36.917524352599955 40.0 36.0 41.0 31.0 41.0 44-45 36.6572605796428 40.0 35.0 41.0 30.5 41.0 46-47 36.281585154050624 39.0 35.0 41.0 30.0 41.0 48-49 35.901273531305065 39.0 35.0 41.0 28.5 41.0 50-51 35.74748618859926 39.0 35.0 41.0 28.0 41.0 52-53 35.680500478963864 39.0 35.0 41.0 28.0 41.0 54-55 35.48122687975844 39.0 35.0 41.0 27.0 41.0 56-57 35.18151066335331 39.0 35.0 41.0 26.0 41.0 58-59 35.02189919990734 39.0 35.0 41.0 26.0 41.0 60-61 34.84516885556593 38.0 35.0 40.0 26.0 41.0 62-63 34.45485357818295 38.0 34.0 40.0 25.5 41.0 64-65 34.18249022600837 37.5 34.0 40.0 25.0 41.0 66-67 33.74501914191244 37.0 34.0 39.5 23.5 41.0 68-69 33.41708732719523 36.5 33.5 39.0 22.5 41.0 70-71 32.91006429898529 36.0 33.0 39.0 22.0 40.5 72-73 32.55368655725832 35.0 33.0 38.5 21.5 39.5 74-75 32.16901633003839 35.0 33.0 37.0 20.5 39.0 76-77 31.649861103807012 35.0 32.0 37.0 20.0 39.0 78-79 31.25280837917163 35.0 32.0 36.0 19.0 37.0 80-81 31.005068895573896 35.0 32.0 36.0 17.5 37.0 82-83 30.854913556840927 35.0 32.0 35.5 16.5 37.0 84-85 30.594027363848113 35.0 32.0 35.0 11.5 36.0 86-87 30.280486432907445 35.0 31.0 35.0 7.0 36.0 88-89 30.086114641116602 34.5 31.0 35.0 7.0 36.0 90-91 29.89221715336363 34.0 31.0 35.0 3.5 35.5 92-93 29.638868899701173 34.0 30.5 35.0 2.0 35.0 94-95 29.578224884685874 34.0 31.0 35.0 2.0 35.0 96-97 29.50715217204622 34.0 31.0 35.0 2.0 35.0 98-99 29.367434517750954 34.0 30.0 35.0 2.0 35.0 100-101 29.096075691601854 34.0 30.0 35.0 2.0 35.0 102-103 27.6235004503392 32.5 26.0 34.5 2.0 35.0 104-105 28.47691121228118 34.0 29.0 35.0 2.0 35.0 106-107 28.55206859801052 34.0 29.0 35.0 2.0 35.0 108-109 28.50737917229452 34.0 29.0 35.0 2.0 35.0 110-111 28.46910300286047 34.0 29.0 35.0 2.0 35.0 112-113 28.43567239004634 34.0 29.0 35.0 2.0 35.0 114-115 28.271888382446825 34.0 29.0 35.0 2.0 35.0 116-117 27.970017660752454 34.0 29.0 35.0 2.0 35.0 118-119 27.76062490971581 34.0 29.0 35.0 2.0 35.0 120-121 27.509525023615346 34.0 29.0 35.0 2.0 35.0 122-123 27.088025836755534 34.0 26.0 35.0 2.0 35.0 124-125 25.949774364415948 33.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2533469204632013 1101 2 0.5674295349993557 1101 3 0.48178199113896625 1101 4 0.5212273235628615 1101 5 0.2286320812888647 1101 6 0.23878473362202612 1101 7 0.21896017469005358 1101 8 0.27405185929973186 1101 9 0.3654229764382819 1101 10-11 0.33302826525542883 1101 12-13 0.29495057012159975 1101 14-15 0.4095295511319321 1101 16-17 0.4457970230116004 1101 18-19 0.3143529453438916 1101 20-21 0.2610390814473291 1101 22-23 0.2607243207003904 1101 24-25 0.34810828257739956 1101 26-27 0.37858850432429136 1101 28-29 0.5034843327290801 1101 30-31 0.3726995031651512 1101 32-33 0.4179852058483391 1101 34-35 0.41891379809352003 1101 36-37 0.46453224291817463 1101 38-39 0.4467870815355184 1101 40-41 0.49194787972935217 1101 42-43 0.655972155936297 1101 44-45 0.6369204696912405 1101 46-47 0.6572546224845581 1101 48-49 0.6747289775785106 1101 50-51 0.6073680751887878 1101 52-53 0.6516081379895411 1101 54-55 0.7339469558846048 1101 56-57 0.6703912613172704 1101 58-59 0.6281907396227595 1101 60-61 0.6106897127686466 1101 62-63 0.8037105060588559 1101 64-65 0.6531519966799664 1101 66-67 0.7362474890409558 1101 68-69 0.692639141142493 1101 70-71 0.6583523887266409 1101 72-73 0.6420733008860822 1101 74-75 0.7216100014861624 1101 76-77 0.7390663055719386 1101 78-79 0.6741563477488945 1101 80-81 0.7058726656899594 1101 82-83 0.6119286878243422 1101 84-85 0.6385202117800475 1101 86-87 0.6073018789790403 1101 88-89 0.6174235545180551 1101 90-91 0.7125241202541979 1101 92-93 0.7113155961257824 1101 94-95 0.8074603807142076 1101 96-97 0.795331527629429 1101 98-99 0.7998724118462093 1101 100-101 0.7644408851811804 1101 102-103 0.714831626735549 1101 104-105 0.8642837726183821 1101 106-107 0.8928087195505476 1101 108-109 0.8501444433209677 1101 110-111 0.7373016588474322 1101 112-113 0.7164060924442417 1101 114-115 0.7259031949828731 1101 116-117 0.7152217355760939 1101 118-119 0.8003937548118571 1101 120-121 0.9271770522060869 1101 122-123 1.126668209241224 1101 124-125 1.0191368165072987 1102 1 0.34135368507336494 1102 2 0.5681900140014946 1102 3 0.4209420105328405 1102 4 0.4598526830469254 1102 5 0.21793265646337545 1102 6 0.18571392605310422 1102 7 0.2036260620594561 1102 8 0.16869438435568895 1102 9 0.2690293571559863 1102 10-11 0.20151645624525116 1102 12-13 0.12656798276614722 1102 14-15 0.3154119724439326 1102 16-17 0.36856274234503417 1102 18-19 0.22530674873515721 1102 20-21 0.26479622961786475 1102 22-23 0.12593004023308652 1102 24-25 0.1694266426658686 1102 26-27 0.13325474038494178 1102 28-29 0.2931575094059582 1102 30-31 0.36228691839996685 1102 32-33 0.31658869741001894 1102 34-35 0.25548781528234343 1102 36-37 0.17913842106287348 1102 38-39 0.22221891903875246 1102 40-41 0.2744838862515451 1102 42-43 0.2630328215712794 1102 44-45 0.3859991360966717 1102 46-47 0.4990851601996624 1102 48-49 0.429825473729629 1102 50-51 0.3796307658896936 1102 52-53 0.3300523357672134 1102 54-55 0.3623853006306632 1102 56-57 0.34045545371512986 1102 58-59 0.3999054869378327 1102 60-61 0.47493902202789684 1102 62-63 0.5166444191052122 1102 64-65 0.5392109055752456 1102 66-67 0.5196095617281387 1102 68-69 0.5591734158314949 1102 70-71 0.5078688701995162 1102 72-73 0.411205242332521 1102 74-75 0.4285125937565013 1102 76-77 0.3973731688119102 1102 78-79 0.3419361973799475 1102 80-81 0.334498016871855 1102 82-83 0.38971232324605865 1102 84-85 0.3839762252096399 1102 86-87 0.3876396777742315 1102 88-89 0.3972582416589816 1102 90-91 0.4432354753015879 1102 92-93 0.422639510367663 1102 94-95 0.49243465228159167 1102 96-97 0.49194918103697205 1102 98-99 0.43750977129042923 1102 100-101 0.4080701300819882 1102 102-103 0.3565775238018922 1102 104-105 0.4426278461955526 1102 106-107 0.48352738881191826 1102 108-109 0.41121003546512114 1102 110-111 0.29128155430647595 1102 112-113 0.2962819168499671 1102 114-115 0.3224315170141807 1102 116-117 0.34376676323893207 1102 118-119 0.4445672071208584 1102 120-121 0.34583568679758514 1102 122-123 0.40876212507711784 1102 124-125 0.45293425512103624 1103 1 0.22234710907948596 1103 2 0.5197454734897207 1103 3 0.37691610560624866 1103 4 0.4347732889362277 1103 5 0.06269742543067025 1103 6 0.11684140556027955 1103 7 0.18487990706220359 1103 8 0.20732211326844663 1103 9 0.27925103715199384 1103 10-11 0.19526656016424937 1103 12-13 0.22773083629856217 1103 14-15 0.3512594526161905 1103 16-17 0.3444763981320733 1103 18-19 0.22478819295975683 1103 20-21 0.1653068875497894 1103 22-23 0.2578839874921144 1103 24-25 0.23759116305893002 1103 26-27 0.2042669529450123 1103 28-29 0.38058310340692003 1103 30-31 0.40884428999765987 1103 32-33 0.40129701758027636 1103 34-35 0.39665189505887355 1103 36-37 0.3968595191125175 1103 38-39 0.3256587416282457 1103 40-41 0.4140023048538737 1103 42-43 0.4495204427408197 1103 44-45 0.2754125965160057 1103 46-47 0.24445110054185193 1103 48-49 0.17214845811683688 1103 50-51 0.18647965104673148 1103 52-53 0.22863540029675278 1103 54-55 0.17917175125666773 1103 56-57 0.2524597601182066 1103 58-59 0.07923828543474798 1103 60-61 0.1031577050001431 1103 62-63 0.08588616378040825 1103 64-65 0.0951203630349653 1103 66-67 0.11805379048293929 1103 68-69 0.08807037807149243 1103 70-71 0.011801164897150329 1103 72-73 0.10527084431446099 1103 74-75 -0.07075207544855999 1103 76-77 0.02938718124893569 1103 78-79 -0.02771758610275299 1103 80-81 -0.18878638447160867 1103 82-83 -0.0826267270174803 1103 84-85 -0.046550498598616485 1103 86-87 -0.11057956636058108 1103 88-89 -0.11733780443116615 1103 90-91 -0.13082000497043111 1103 92-93 -0.16061560660355312 1103 94-95 -0.0386754727537415 1103 96-97 0.16571555867027854 1103 98-99 -0.03311139409872865 1103 100-101 -0.03889959208019178 1103 102-103 -0.11997315541264442 1103 104-105 0.03973484534757432 1103 106-107 0.09140902092206105 1103 108-109 0.08758311712050926 1103 110-111 -0.03027831134705039 1103 112-113 0.17337370226140436 1103 114-115 0.09653874894474868 1103 116-117 0.10822189517448777 1103 118-119 0.21896510047502815 1103 120-121 0.15444350483127067 1103 122-123 0.2962239868066625 1103 124-125 0.3286775876784418 1104 1 0.2133509192064551 1104 2 0.548943508158807 1104 3 0.0632139492582553 1104 4 0.3936535406293302 1104 5 0.15168891648760052 1104 6 0.13628837533462956 1104 7 0.15301069758693586 1104 8 0.08722037368500679 1104 9 0.2185944286757433 1104 10-11 0.2371212645984926 1104 12-13 0.251155966792858 1104 14-15 0.2788706153688807 1104 16-17 0.3115310576211314 1104 18-19 0.24335566693751076 1104 20-21 0.22587156266752118 1104 22-23 0.22627065919809297 1104 24-25 0.2174240486427621 1104 26-27 0.2615250916009444 1104 28-29 0.37315284507418056 1104 30-31 0.3233258369296408 1104 32-33 0.282436378336989 1104 34-35 0.35302083384147664 1104 36-37 0.38558338194795283 1104 38-39 0.41697461443334305 1104 40-41 0.4314691322336728 1104 42-43 0.39314231062236615 1104 44-45 0.24406740718436026 1104 46-47 0.24878218321036627 1104 48-49 0.42312295171725367 1104 50-51 0.34379601416306826 1104 52-53 0.40655658276403983 1104 54-55 0.403365546776449 1104 56-57 0.5138155728601745 1104 58-59 0.5316168057162685 1104 60-61 0.514209626918074 1104 62-63 0.49346862289559823 1104 64-65 0.48276962705735116 1104 66-67 0.45342617165906773 1104 68-69 0.3908319540135494 1104 70-71 0.3003191991576486 1104 72-73 0.25679744880694244 1104 74-75 0.3234055596168659 1104 76-77 0.284636065280111 1104 78-79 0.17066234382359724 1104 80-81 0.28469952418749145 1104 82-83 0.24893501644759297 1104 84-85 0.1976183727661862 1104 86-87 0.2010975023953563 1104 88-89 0.2690136670133114 1104 90-91 0.21170070557228016 1104 92-93 0.03133767082067607 1104 94-95 0.1363217181714127 1104 96-97 0.13542504925732146 1104 98-99 0.22166352239513643 1104 100-101 0.19159112775800224 1104 102-103 0.08354431703288157 1104 104-105 0.14684231544369553 1104 106-107 0.21983634760213988 1104 108-109 0.18554168582026165 1104 110-111 0.12651198866214486 1104 112-113 0.11201073914711301 1104 114-115 0.1803962726686592 1104 116-117 0.13572529160134295 1104 118-119 0.1462156801659269 1104 120-121 0.086191157976927 1104 122-123 0.02949364777686725 1104 124-125 0.1299523969167211 1105 1 0.2117117410756748 1105 2 0.532321762495485 1105 3 0.21256624880436448 1105 4 0.3925818677555739 1105 5 0.1590010034531062 1105 6 0.10993601927185637 1105 7 0.10524690605937082 1105 8 0.05148118444577676 1105 9 0.1338110531497989 1105 10-11 0.1119439679269405 1105 12-13 0.18903626060155432 1105 14-15 0.21026766831719357 1105 16-17 0.22409247077158767 1105 18-19 0.18401063660365224 1105 20-21 0.1575497372962147 1105 22-23 0.11769328106458943 1105 24-25 0.11462471982685685 1105 26-27 0.1315926972237662 1105 28-29 0.22739052297986717 1105 30-31 0.21185724168054776 1105 32-33 0.14708111264046408 1105 34-35 0.17070031809767983 1105 36-37 0.19353235324722817 1105 38-39 0.1258107999093383 1105 40-41 0.1801016238584836 1105 42-43 0.19307712157413448 1105 44-45 0.3294465462991525 1105 46-47 0.2604973080439805 1105 48-49 0.25468214902472397 1105 50-51 0.34379562439297473 1105 52-53 0.31766512788870216 1105 54-55 0.3506863645920646 1105 56-57 0.3274307294600547 1105 58-59 0.3046959699712346 1105 60-61 0.3951854358371918 1105 62-63 0.3312806094953018 1105 64-65 0.3137462155166517 1105 66-67 0.464962879232111 1105 68-69 0.3959462248608929 1105 70-71 0.40303346065051215 1105 72-73 0.35712232218038764 1105 74-75 0.3839568309494652 1105 76-77 0.31856867013313916 1105 78-79 0.36033168236425794 1105 80-81 0.2941663576757563 1105 82-83 0.3038830503497927 1105 84-85 0.42994590437166735 1105 86-87 0.3272390902797433 1105 88-89 0.4457622243767503 1105 90-91 0.4127206364660445 1105 92-93 0.4856986135186254 1105 94-95 0.3581809994743388 1105 96-97 0.4061132663124951 1105 98-99 0.4669220666428089 1105 100-101 0.4625767393949829 1105 102-103 0.3563409954121397 1105 104-105 0.42174346000357943 1105 106-107 0.25199325734892497 1105 108-109 0.36407714418015047 1105 110-111 0.3512836321082595 1105 112-113 0.4171127322364896 1105 114-115 0.5544358304355157 1105 116-117 0.4638902734867756 1105 118-119 0.5869789084757109 1105 120-121 0.5291564898759056 1105 122-123 0.5972581019135959 1105 124-125 0.5132661443028255 1106 1 0.2842679439946991 1106 2 0.5335248173267217 1106 3 0.4130819892433024 1106 4 0.4846619642715808 1106 5 0.19695042308948985 1106 6 0.17723295991360288 1106 7 0.16607473906675807 1106 8 0.10666326107912738 1106 9 0.29433285979183665 1106 10-11 0.20267755975295643 1106 12-13 0.21784053953824412 1106 14-15 0.27624017347799423 1106 16-17 0.24777471264105344 1106 18-19 0.1562266341771732 1106 20-21 0.23803699147917712 1106 22-23 0.18308547920307205 1106 24-25 0.20307329970155052 1106 26-27 0.20792811072152517 1106 28-29 0.2751754534448807 1106 30-31 0.18023602626695379 1106 32-33 0.2748776775397772 1106 34-35 0.29965410177869245 1106 36-37 0.2941637023889001 1106 38-39 0.3683627247970662 1106 40-41 0.5125782191793604 1106 42-43 0.38841140784459327 1106 44-45 0.45702128735376846 1106 46-47 0.43705720500906864 1106 48-49 0.30738370314973906 1106 50-51 0.3102195863479196 1106 52-53 0.42353621654579854 1106 54-55 0.37819563365985687 1106 56-57 0.4764708886299829 1106 58-59 0.3578301371811463 1106 60-61 0.31670421616820477 1106 62-63 0.3501789258975947 1106 64-65 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64-65 -1.3844915238001363 2216 66-67 -1.3572794983112217 2216 68-69 -1.530640011423614 2216 70-71 -1.5198501738864323 2216 72-73 -1.2647989205095342 2216 74-75 -1.1382648034334686 2216 76-77 -1.1655818441942216 2216 78-79 -1.0329595116228774 2216 80-81 -1.1121941622931146 2216 82-83 -1.1778398332498234 2216 84-85 -1.1498163489780993 2216 86-87 -1.2310958974428523 2216 88-89 -1.3127834600768296 2216 90-91 -1.3203748664063077 2216 92-93 -1.290399137053253 2216 94-95 -1.3032205187923012 2216 96-97 -1.5055186005597179 2216 98-99 -1.4270271290271737 2216 100-101 -1.6651542469580107 2216 102-103 -1.7178882643521192 2216 104-105 -1.8117568662472259 2216 106-107 -1.8755653931513443 2216 108-109 -1.90186649763087 2216 110-111 -1.9232891993453585 2216 112-113 -1.890768935366939 2216 114-115 -2.0255507912736697 2216 116-117 -2.195838521913551 2216 118-119 -2.1898103680786356 2216 120-121 -2.2590169054843656 2216 122-123 -2.3660872669925155 2216 124-125 -2.6117885727669297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28.0 3 18.0 4 59.0 5 227.0 6 747.0 7 1275.0 8 2051.0 9 4141.0 10 8492.0 11 10856.0 12 9739.0 13 11958.0 14 16591.0 15 16533.0 16 9729.0 17 8506.0 18 8716.0 19 8923.0 20 9286.0 21 8907.0 22 9282.0 23 10059.0 24 11251.0 25 12867.0 26 15291.0 27 18551.0 28 22703.0 29 27939.0 30 34685.0 31 43328.0 32 55647.0 33 73557.0 34 103810.0 35 164849.0 36 248666.0 37 361066.0 38 150856.0 39 1012.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.51556314657557 17.930584832982007 10.950468594376868 23.603383426065555 2 11.886249349286846 24.205663967066922 35.86730910306098 28.040777580585246 3 22.932447698340898 29.82121543151074 28.580767261860778 18.665569608287583 4 13.708884496815008 19.694701308280315 32.56894383641071 34.02747035849397 5 10.449334010561834 39.70107861730887 36.02267605999464 13.82691131213466 6 31.896545411334575 29.036039748440068 20.121133284558702 18.946281555666655 7 21.56748664126838 33.33129188437499 25.09224797480497 20.008973499551658 8 28.347804321791827 31.520082865075977 24.147767176296647 15.98434563683555 9 29.32983002940352 13.866253583907879 21.634122198028095 35.16979418866051 10-11 23.039534336461745 27.671572469853388 26.531711312837402 22.75718188084746 12-13 27.792070497485394 25.52102039478086 26.46514985941578 20.221759248317966 14-15 28.460508674737618 22.577517444670338 26.64987105389641 22.312102826695636 16-17 21.770652486499728 26.735830040367674 33.39909279239938 18.094424680733223 18-19 24.14014502719676 24.662112460316564 33.982802567699 17.214939944787684 20-21 20.078671360675703 25.049252811795515 34.747072374256796 20.12500345327199 22-23 18.638983731205077 26.74891708899142 29.177486900887434 25.43461227891607 24-25 20.359958487579224 28.37469819285169 27.611584601528023 23.65375871804106 26-27 22.90365936382681 23.241796537214395 29.455711985280264 24.39883211367853 28-29 21.810140873573257 22.35381088245358 33.29627652280192 22.539771721171245 30-31 24.38295541009492 25.004310341958234 31.049173845577254 19.56356040236959 32-33 18.158056079046677 25.332961434588313 33.35419161616854 23.154790870196464 34-35 16.283051524430835 27.069597923046203 34.971641592331245 21.67570896019172 36-37 23.390844500835772 24.800476101400545 30.758866489903813 21.049812907859867 38-39 25.074540624057633 22.950224370773288 30.361649339868634 21.613585665300448 40-41 20.805478363241125 25.996130343452823 30.634658955312556 22.563732337993496 42-43 24.578202251230028 24.712272192602722 29.79082026972422 20.918705286443025 44-45 26.70890912734048 22.034401521500786 29.52797262150671 21.728716729652024 46-47 21.79585155382003 24.75713969036101 35.30346471610656 18.143544039712395 48-49 24.317085396694583 24.61974129959972 32.946423281571505 18.116750022134188 50-51 19.826075205648245 25.696228400859805 32.992755297060775 21.48494109643117 52-53 18.66591088675883 27.384717491201243 27.579365211446405 26.370006410593522 54-55 20.52268637818774 28.474618243497375 25.995489285388572 25.007206092926314 56-57 23.094046668854567 23.84108384963131 27.48610205957791 25.57876742193621 58-59 22.29798142858379 22.85270080368739 31.135813383162443 23.71350438456638 60-61 24.47641826892673 25.366245928474285 29.145300795299693 21.01203500729929 62-63 18.75837521077406 25.795283054664452 31.334255535710604 24.112086198850886 64-65 16.722495857744736 27.181149526594645 33.09879969458148 22.997554921079136 66-67 23.334127723254078 25.304170347376946 29.097038279165037 22.264663650203932 68-69 25.00407735050103 23.527677055201003 28.726382155250864 22.741863439047105 70-71 20.93591336978207 26.19835827562357 29.148263115255546 23.71746523933881 72-73 24.528075803437755 25.024713736710336 28.34304463916613 22.104165820685783 74-75 26.29957642153081 22.526479479110982 28.34913570154726 22.824808397810944 76-77 21.634588180942497 24.97335576264428 34.108817661551285 19.28323839486194 78-79 23.861820089322265 24.82164503951202 32.04644385138873 19.27009101977698 80-81 19.593749438324163 25.79029038058156 32.19615750488783 22.419802676206444 82-83 18.491932837216858 27.22761468005946 27.12110429962435 27.15934818309933 84-85 20.16148305053718 28.084890104586535 25.819813726658413 25.93381311821787 86-87 22.446197279858023 23.660914884226543 27.37020545186696 26.522682384048473 88-89 21.837557024659148 22.758938384410605 30.627625730511433 24.775878860418814 90-91 23.7177315152899 25.162478256904368 28.680283131218793 22.43950709658694 92-93 18.517062630100767 25.64264036570339 30.485500941618334 25.354796062577513 94-95 16.73320299004052 26.849586636018017 32.17765165056015 24.23955872338131 96-97 22.817577807357495 25.00260459053673 28.61654469194434 23.56327291016143 98-99 24.060699966880637 23.471641338954193 28.277643432630395 24.190015261534775 100-101 20.58133365641482 25.63275487101926 28.69679224018623 25.089119232379687 102-103 23.613448510928382 24.69343817902944 28.126963859656595 23.56614945038558 104-105 25.13095158037434 22.33917108498553 28.144086355134963 24.38579097950517 106-107 21.005992210126838 24.753154232830653 33.11028995639003 21.130563600652486 108-109 23.08367600764892 25.008289433735133 30.823410426176768 21.084624132439185 110-111 19.24214788792175 25.789821823826532 31.249090776537592 23.718939511714126 112-113 18.321470111805677 26.899241734748973 26.734825540402973 28.044462613042377 114-115 19.55635722377132 27.914425432757657 25.551564973418195 26.97765237005283 116-117 21.616239108769626 23.957560204567166 26.847392747581843 27.57880793908137 118-119 20.923066269648917 23.351015804360447 29.621190733648316 26.104727192342313 120-121 22.6619054688972 25.434503126824648 27.911395649675804 23.992195754602356 122-123 18.270542348216193 26.01312915528578 29.053132403956187 26.663196092541842 124-125 16.713454442331212 27.297088950473746 30.474019969737178 25.515436637457867 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2856.0 1 2194.5 2 4040.0 3 5292.0 4 2603.5 5 1355.0 6 2268.0 7 4751.5 8 6978.5 9 7033.5 10 5073.5 11 2833.0 12 2177.5 13 2219.5 14 2451.0 15 2822.0 16 2981.0 17 3293.5 18 3631.0 19 3608.5 20 3623.5 21 3975.0 22 4513.0 23 4988.5 24 5533.0 25 6312.0 26 7438.0 27 8753.5 28 9712.0 29 10847.0 30 12459.5 31 13942.0 32 15523.5 33 17435.0 34 19565.0 35 20991.0 36 22275.5 37 23915.5 38 24229.0 39 23572.5 40 23124.5 41 23492.0 42 24386.5 43 25307.5 44 26541.5 45 27949.5 46 31970.0 47 43531.0 48 57674.0 49 102304.5 50 259019.5 51 256134.5 52 88013.0 53 33100.5 54 22492.5 55 18350.0 56 16238.5 57 14532.0 58 13400.5 59 12215.5 60 10855.5 61 9526.0 62 8202.5 63 6237.5 64 4442.0 65 3478.5 66 2376.5 67 1714.0 68 1320.5 69 928.5 70 803.0 71 757.0 72 750.0 73 772.0 74 563.0 75 422.5 76 309.5 77 147.0 78 85.5 79 48.5 80 16.0 81 10.5 82 5.5 83 2.0 84 4.0 85 3.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14532010030615078 2 1.9970696331582792E-4 3 1.3313797554388525E-4 4 0.0 5 0.0 6 0.003594725339684902 7 0.0 8 0.0 9 0.0 10-11 3.328449388597131E-5 12-13 0.005092527564553612 14-15 0.0015310867187546806 16-17 5.991208899474837E-4 18-19 0.0 20-21 1.6642246942985659E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.9970696331582792E-4 30-31 0.0 32-33 0.0 34-35 6.656898777194263E-5 36-37 0.0 38-39 0.0 40-41 3.328449388597131E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 9.319658288071968E-4 96-97 0.00302888894362339 98-99 0.0038942857846586444 100-101 0.0 102-103 0.0037944323030007308 104-105 0.020636386209302218 106-107 0.0167088159307576 108-109 0.019238437466091423 110-111 0.015477289656976662 112-113 0.01464517730982738 114-115 0.040607082540885006 116-117 0.03937555626710407 118-119 0.022433748879144667 120-121 0.013180659578844643 122-123 0.0031953114130532467 124-125 0.0018639316576143936 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1502201.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.24010434704298 #Duplication Level Percentage of deduplicated Percentage of total 1 85.84782155096181 43.98851334237608 2 9.795461690368121 10.038409582838492 3 2.209613245796551 3.3966243984367086 4 0.7767960450889628 1.5921244162691504 5 0.37856625744225597 0.9698887266805366 6 0.21266605684146533 0.6538218566178514 7 0.1290801678275913 0.4629856888033719 8 0.10069362477592905 0.4127641472480478 9 0.07478698113147637 0.3448883445279465 >10 0.40428896040355605 3.8683954910875435 >50 0.033695677296625745 1.1767675216934428 >100 0.027097883253668423 2.960876701915763 >500 0.004042225205167571 1.387884501972908 >1k 0.002395392714173376 2.5813792555487702 >5k 0.0014971204463583598 6.252154255764646 >10k+ 0.0014971204463583598 19.91252176821875 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 79849 5.315467104601847 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 43058 2.8663274754843058 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 28865 1.9215138320371243 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 21895 1.457527987266684 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 20873 1.3894944817637587 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 19358 1.2886424652892656 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 13986 0.9310338629783896 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 10978 0.7307943477603862 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 10375 0.6906532481339048 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10336 0.6880570576107992 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9135 0.608107703296696 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 9075 0.6041135640303794 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 9047 0.602249632372765 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 9038 0.6016505114828176 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8519 0.5671012068291793 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 8075 0.5375445762584368 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 7643 0.5087867735409576 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7327 0.48775097340502366 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 7308 0.4864861626373567 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 6334 0.42164796854748465 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 4065 0.2706029352929468 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3882 0.25842081053068133 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3436 0.2287310419843949 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3174 0.21128996718814594 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2857 0.1901875980644401 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2367 0.1575687940561882 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 2239 0.14904796362137956 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 1945 0.12947668121642844 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.9956044497374187E-4 0.0 12-13 0.0 0.0 0.0 0.0010983882982370536 0.0 14-15 0.0 0.0 0.0 0.002230061090360078 0.0 16-17 0.0 0.0 0.0 0.0041272772418604435 0.0 18-19 0.0 0.0 0.0 0.005991208899474837 0.0 20-21 0.0 0.0 0.0 0.00838769245926477 0.0 22-23 0.0 0.0 0.0 0.012714676664441045 0.0 24-25 0.0 0.0 0.0 0.017574212771792858 0.0 26-27 0.0 0.0 0.0 0.02266674033634647 0.0 28-29 0.0 0.0 0.0 0.03205296761219038 0.0 30-31 0.0 0.0 0.0 0.04912791297569366 0.0 32-33 0.0 0.0 0.0 0.08038205273462073 0.0 34-35 0.0 0.0 0.0 0.12747961158327015 0.0 36-37 0.0 0.0 0.0 0.19448129777573042 0.0 38-39 0.0 0.0 0.0 0.2977963667977854 0.0 40-41 0.0 0.0 0.0 0.4513710215876571 0.0 42-43 0.0 0.0 0.0 0.5703963717238905 0.0 44-45 0.0 0.0 0.0 0.6850947376549477 0.0 46-47 0.0 0.0 0.0 0.841265582967925 0.0 48-49 0.0 0.0 0.0 0.9836566478121103 0.0 50-51 0.0 0.0 0.0 1.1253487382846903 0.0 52-53 0.0 0.0 0.0 1.30661609198769 0.0 54-55 0.0 0.0 0.0 1.492643128316384 0.0 56-57 0.0 0.0 0.0 1.6902531685173954 0.0 58-59 0.0 0.0 0.0 1.931033197288512 0.0 60-61 0.0 0.0 0.0 2.1546051427205812 0.0 62-63 0.0 0.0 0.0 2.3684580159379474 0.0 64-65 0.0 0.0 0.0 2.6235170925861455 0.0 66-67 0.0 0.0 0.0 2.9002776592479966 0.0 68-69 0.0 0.0 0.0 3.2428749548162994 0.0 70-71 0.0 0.0 0.0 3.756155135031863 0.0 72-73 0.0 0.0 0.0 4.152473603732124 0.0 74-75 0.0 0.0 0.0 4.511679861749526 0.0 76-77 0.0 0.0 0.0 4.936656279685607 0.0 78-79 0.0 0.0 0.0 5.341196018375703 0.0 80-81 0.0 0.0 0.0 5.774360421807734 0.0 82-83 0.0 0.0 0.0 6.337367635888938 0.0 84-85 0.0 0.0 0.0 6.862363957952365 0.0 86-87 0.0 0.0 0.0 7.371050878011665 0.0 88-89 0.0 0.0 0.0 7.968740534722052 0.0 90-91 0.0 0.0 0.0 8.531015489937765 0.0 92-93 0.0 0.0 0.0 9.08979557329545 0.0 94-95 0.0 0.0 0.0 9.716176463735547 0.0 96-97 0.0 0.0 0.0 10.331806462650471 0.0 98-99 0.0 0.0 0.0 11.053980126494391 0.0 100-101 0.0 0.0 0.0 12.120415310600912 0.0 102-103 0.0 0.0 0.0 12.975127829098769 0.0 104-105 0.0 0.0 0.0 13.72133289752836 0.0 106-107 0.0 0.0 0.0 14.531777039157877 0.0 108-109 0.0 0.0 0.0 15.24316652698274 0.0 110-111 0.0 0.0 0.0 15.992267346380412 0.0 112-113 0.0 0.0 0.0 16.93951075788127 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1635 0.0 47.671062 5 AAGTACT 1920 0.0 40.594887 4 GTTATCA 660 0.0 39.730038 1 AAAGTAC 1965 0.0 39.362446 3 AAAAGTA 2190 0.0 34.50332 2 AAAAAGT 2320 0.0 33.136906 1 TTATCAA 835 0.0 30.639612 2 CGCATGT 175 5.6233876E-7 27.243456 1 AGTGCGC 90 0.009530005 26.44437 8 TAGTACA 420 0.0 24.082464 4 CATATAG 275 2.1191227E-9 23.79914 3 CGTGTAC 240 3.5657285E-7 22.311693 3 GCAACGC 135 0.0025561799 22.036242 3 TTACTCT 775 0.0 21.496 4 CGGGAGT 610 0.0 21.458242 4 GTATAGA 205 5.8660065E-5 20.349531 1 GTATAGG 180 5.7228835E-4 19.865017 1 ACTTGAC 360 2.1809683E-9 19.833279 8 CCGGGTC 675 0.0 19.39254 9 TTTACTC 900 0.0 19.17153 3 >>END_MODULE