FastQCFastQC Report
Thu 26 May 2016
SRR1364165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2765578
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA505401.827466084847363No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG259630.938791095387655No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG127130.4596869081255347No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC120660.4362921602645089No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN100940.36498699367727105No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG94960.3433640273389505No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT86390.31237593009490244No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC61760.22331678947402678No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT57390.20751539099602329No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG50210.18155336786740423No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA49950.18061323889617287No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG48160.17414081251731103No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC42000.15186698766044565No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG40220.14543072008816965No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG35560.12858071621917733No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA34870.1260857585647557No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA33750.12203597222714385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG95900.025.8882884
ATGGGGG43900.022.9213225
ATGGGGA51050.021.3438535
CATGGGT50500.018.3915844
GTGTAGC12800.018.1391471
AGTACTC17650.016.5298485
ATTCGCG3700.016.07993580-81
ATGGGAT33700.016.0778875
TATACAG16350.016.0233155
GTATAGG9800.015.7946311
CCGTCTA2654.220362E-415.7250019
ATGGGTA28350.015.5416145
ATGGGAG32600.015.5245435
GTACTAT8350.014.9725251
TGGGGAG34250.014.947776
TACATGG896700.014.7517412
GTAATAC5251.4186298E-814.7424583
GTACATG906000.014.6796991
ACATGGG886450.014.3729343
CTCGCGC4150.014.33411460-61
Produced by FastQC (version 0.11.5)