##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364165_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2765578 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.858184075806214 31.0 31.0 34.0 28.0 34.0 2 30.95125575919392 33.0 31.0 34.0 27.0 34.0 3 31.02905143156331 33.0 31.0 34.0 27.0 34.0 4 34.50943057834565 37.0 35.0 37.0 32.0 37.0 5 34.474702214148365 37.0 35.0 37.0 31.0 37.0 6 34.45605114012333 37.0 35.0 37.0 31.0 37.0 7 34.3748286976538 37.0 35.0 37.0 30.0 37.0 8 34.32391167415998 37.0 35.0 37.0 30.0 37.0 9 35.7919230627377 39.0 37.0 39.0 30.0 39.0 10-11 35.71303195932279 39.0 37.0 39.0 30.0 39.0 12-13 35.63644760697402 39.0 37.0 39.0 30.0 39.0 14-15 36.891384007249115 40.0 37.0 41.0 30.0 41.0 16-17 36.92976061423688 40.0 37.0 41.0 30.0 41.0 18-19 36.929316403297975 40.0 37.0 41.0 30.0 41.0 20-21 36.940785976746994 40.0 37.0 41.0 30.0 41.0 22-23 36.90975828560974 40.0 37.0 41.0 30.0 41.0 24-25 36.89290647380041 40.0 37.0 41.0 30.0 41.0 26-27 36.800280266909844 40.0 37.0 41.0 30.0 41.0 28-29 36.739810809892184 40.0 37.0 41.0 30.0 41.0 30-31 36.81733113294942 40.0 37.0 41.0 30.0 41.0 32-33 36.62226268794444 40.0 37.0 41.0 30.0 41.0 34-35 36.69326900199525 40.0 37.0 41.0 30.0 41.0 36-37 36.68753891591559 40.0 37.0 41.0 30.0 41.0 38-39 36.614207229013246 40.0 36.5 41.0 30.0 41.0 40-41 36.47117401859575 40.0 36.0 41.0 30.0 41.0 42-43 36.35021792912729 40.0 36.0 41.0 30.0 41.0 44-45 36.20711909047584 40.0 36.0 41.0 29.0 41.0 46-47 36.05046630396973 40.0 35.0 41.0 29.0 41.0 48-49 35.90436013737454 40.0 35.0 41.0 28.0 41.0 50-51 35.784526055674434 39.5 35.0 41.0 28.0 41.0 52-53 35.639254615129275 39.0 35.0 41.0 28.0 41.0 54-55 35.44410119692881 39.0 35.0 41.0 27.0 41.0 56-57 35.19734012202874 39.0 34.5 41.0 27.0 41.0 58-59 34.944866317276166 38.5 34.0 41.0 26.0 41.0 60-61 34.681358471899905 38.0 34.0 40.0 26.0 41.0 62-63 34.311558379477994 37.5 34.0 40.0 25.5 41.0 64-65 34.06392678130937 37.0 33.0 40.0 25.0 41.0 66-67 33.74305732834149 36.5 33.0 40.0 24.5 41.0 68-69 33.39920461473153 36.0 33.0 39.0 23.5 41.0 70-71 33.07607234364751 36.0 33.0 39.0 23.0 41.0 72-73 32.88640060052546 35.0 33.0 39.0 23.0 40.5 74-75 32.577222555285005 35.0 33.0 37.5 22.0 40.0 76-77 32.13028795427213 35.0 33.0 37.0 20.0 39.0 78-79 31.758426629080795 35.0 32.0 36.5 20.0 39.0 80-81 31.509976395531062 35.0 32.0 36.0 20.0 38.5 82-83 31.270657526202477 35.0 32.0 36.0 18.0 37.0 84-85 31.022552247667576 35.0 32.0 35.5 17.5 37.0 86-87 30.75879183302731 35.0 32.0 35.0 12.5 36.5 88-89 30.537264362097183 35.0 32.0 35.0 8.5 36.0 90-91 30.372389062973454 35.0 32.0 35.0 7.0 36.0 92-93 30.171254254987566 35.0 31.5 35.0 7.0 36.0 94-95 30.11224597534403 35.0 32.0 35.0 2.0 35.5 96-97 30.05710144497823 35.0 32.0 35.0 2.0 35.0 98-99 29.942780858106335 35.0 31.5 35.0 2.0 35.0 100-101 29.724141210264186 35.0 31.0 35.0 2.0 35.0 102-103 28.697956087298927 33.5 29.5 35.0 2.0 35.0 104-105 29.452779310509413 35.0 30.5 35.0 2.0 35.0 106-107 29.48061164790868 35.0 31.0 35.0 2.0 35.0 108-109 29.45249474070158 35.0 31.0 35.0 2.0 35.0 110-111 29.46467863137471 35.0 31.0 35.0 2.0 35.0 112-113 29.395466878894755 35.0 31.0 35.0 2.0 35.0 114-115 29.30493625563987 35.0 31.0 35.0 2.0 35.0 116-117 29.14183599233144 35.0 31.0 35.0 2.0 35.0 118-119 28.990806623425556 35.0 31.0 35.0 2.0 35.0 120-121 28.84050061144542 35.0 31.0 35.0 2.0 35.0 122-123 28.601069649816424 35.0 30.0 35.0 2.0 35.0 124-125 27.747865907235305 34.0 27.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30343180914156775 1101 2 0.39783566627732014 1101 3 0.43384512801359065 1101 4 0.4434387432710096 1101 5 0.3707691646282498 1101 6 0.27041544432425013 1101 7 0.4377657773192283 1101 8 0.4728673234424363 1101 9 0.6400931281887665 1101 10-11 0.5734204892472903 1101 12-13 0.6371998520145894 1101 14-15 0.7075667271468333 1101 16-17 0.6694001064380117 1101 18-19 0.6226098076768452 1101 20-21 0.6652122768816113 1101 22-23 0.6025398416600751 1101 24-25 0.7187712547882796 1101 26-27 0.7854320813808329 1101 28-29 0.8652415447693897 1101 30-31 0.7199158268400438 1101 32-33 0.7391125528115481 1101 34-35 0.6994419161642753 1101 36-37 0.6573522261068945 1101 38-39 0.6750543408371641 1101 40-41 0.6492920596209117 1101 42-43 0.5945289625172379 1101 44-45 0.7015863046561392 1101 46-47 0.7389128313028479 1101 48-49 0.7227510576764189 1101 50-51 0.6971995262873065 1101 52-53 0.7237682063798019 1101 54-55 0.6745339543520359 1101 56-57 0.6509062778301242 1101 58-59 0.7040894400775031 1101 60-61 0.6799974385434524 1101 62-63 0.6719723063524228 1101 64-65 0.6842471916064454 1101 66-67 0.7404228248355835 1101 68-69 0.8161491295564005 1101 70-71 0.6646708261044765 1101 72-73 0.6156306363227344 1101 74-75 0.6555236646182863 1101 76-77 0.6217229853770903 1101 78-79 0.6051446820386097 1101 80-81 0.6523696623515391 1101 82-83 0.6128067425678232 1101 84-85 0.5984058786240638 1101 86-87 0.534945198107593 1101 88-89 0.5357032960118246 1101 90-91 0.548783603580766 1101 92-93 0.5458767575917705 1101 94-95 0.5496015787462909 1101 96-97 0.5483208500422023 1101 98-99 0.5599729598069985 1101 100-101 0.4280046261413126 1101 102-103 0.49417964499735234 1101 104-105 0.532101310839149 1101 106-107 0.47692127982515586 1101 108-109 0.4707275948442273 1101 110-111 0.6010237754468726 1101 112-113 0.5627823587607601 1101 114-115 0.4927366539839788 1101 116-117 0.48744140137801395 1101 118-119 0.521156694652305 1101 120-121 0.5248284518148871 1101 122-123 0.5148394286275746 1101 124-125 0.5210274586511154 1102 1 0.14864497127618392 1102 2 0.2978375914807607 1102 3 0.3255799889958553 1102 4 0.2888201200561795 1102 5 0.2604038693080568 1102 6 0.25304758246721804 1102 7 0.24437439910218473 1102 8 0.2724955185281175 1102 9 0.3910335299065295 1102 10-11 0.28511766437222263 1102 12-13 0.32040197070097776 1102 14-15 0.4189710306984722 1102 16-17 0.38219350443411315 1102 18-19 0.38848016207069946 1102 20-21 0.4060671995666496 1102 22-23 0.4056542191996826 1102 24-25 0.44301840999938946 1102 26-27 0.4935989146979196 1102 28-29 0.5484940797208537 1102 30-31 0.40241897635254986 1102 32-33 0.6134918852713227 1102 34-35 0.5009948935515496 1102 36-37 0.47155571529747675 1102 38-39 0.47169967384321154 1102 40-41 0.46561531812399437 1102 42-43 0.47689458030900056 1102 44-45 0.5705112349577561 1102 46-47 0.502412718798773 1102 48-49 0.4869323468648403 1102 50-51 0.5761103665062492 1102 52-53 0.5229874762223332 1102 54-55 0.42468075111433023 1102 56-57 0.4350985527106772 1102 58-59 0.5142276817170099 1102 60-61 0.5095396853280576 1102 62-63 0.5445024207456015 1102 64-65 0.4934656673025586 1102 66-67 0.4878662148300563 1102 68-69 0.49275616304652203 1102 70-71 0.49470105179848645 1102 72-73 0.41147514486041814 1102 74-75 0.3308193337528209 1102 76-77 0.32722744282468597 1102 78-79 0.3217605113019424 1102 80-81 0.36895637291284444 1102 82-83 0.3113465960839079 1102 84-85 0.2958277304050583 1102 86-87 0.2858071477851851 1102 88-89 0.27351429157062057 1102 90-91 0.36214836586025356 1102 92-93 0.3412376790903977 1102 94-95 0.21396125006593536 1102 96-97 0.22454145910437617 1102 98-99 0.23895064068286942 1102 100-101 0.3156733972959884 1102 102-103 0.15262912224445557 1102 104-105 0.16391926841489024 1102 106-107 0.20000892110199686 1102 108-109 0.1773787515906804 1102 110-111 0.21125214067240705 1102 112-113 0.2790427473390089 1102 114-115 0.29822173907195193 1102 116-117 0.3072474732290118 1102 118-119 0.36184003358075856 1102 120-121 0.3406663785633022 1102 122-123 0.3437340851051012 1102 124-125 0.2599834087931576 1103 1 0.09438424950141666 1103 2 0.012598769089869677 1103 3 0.08065519441134228 1103 4 0.04083342867875217 1103 5 0.06425830395262722 1103 6 0.005309956460202159 1103 7 0.04515581904307453 1103 8 0.07489217754758926 1103 9 0.13938167423054182 1103 10-11 0.18273246163470702 1103 12-13 0.12566584222819444 1103 14-15 0.08917150113111205 1103 16-17 0.14893393602174143 1103 18-19 0.16503523149963684 1103 20-21 0.17670210236227035 1103 22-23 0.19958035953479225 1103 24-25 0.2254394462466749 1103 26-27 0.37017064890831364 1103 28-29 0.39392599605228895 1103 30-31 0.21245509022702436 1103 32-33 0.3481982322448829 1103 34-35 0.166802882882223 1103 36-37 0.20131803611784704 1103 38-39 0.19146040673588516 1103 40-41 0.2681695010203953 1103 42-43 0.21914932403148413 1103 44-45 0.2955845164912958 1103 46-47 0.18317043100343966 1103 48-49 0.23390005755166499 1103 50-51 0.21278930879655888 1103 52-53 0.19801239484406352 1103 54-55 0.2658395226141934 1103 56-57 0.24380837233949393 1103 58-59 0.1499350757077309 1103 60-61 0.1572137579734587 1103 62-63 0.24793136569454077 1103 64-65 0.12630995757864127 1103 66-67 0.16102102835368726 1103 68-69 0.10404626849265242 1103 70-71 0.13379258072390598 1103 72-73 0.0822879463129027 1103 74-75 0.0569843457843362 1103 76-77 0.054201222440624974 1103 78-79 0.0863791742136577 1103 80-81 0.0043813478008658535 1103 82-83 0.12809381926484775 1103 84-85 0.0801454580587837 1103 86-87 0.09459539702360686 1103 88-89 0.07177085814709017 1103 90-91 0.068178781080384 1103 92-93 0.06475523862197718 1103 94-95 0.041470973403974654 1103 96-97 -0.0038966822903141463 1103 98-99 0.05717479371559264 1103 100-101 0.021719885147980733 1103 102-103 -0.019494444632677244 1103 104-105 0.11012247772067596 1103 106-107 0.10821485767963424 1103 108-109 0.057780726905054536 1103 110-111 0.08731988306944416 1103 112-113 0.06880649898615232 1103 114-115 0.14063937227177448 1103 116-117 0.09032325774263228 1103 118-119 0.048694953062916824 1103 120-121 0.05569196909354801 1103 122-123 0.061961210970483904 1103 124-125 0.04987894393687853 1104 1 0.2981021552148171 1104 2 0.3032379036013566 1104 3 0.35536694258697565 1104 4 0.3843048704992498 1104 5 0.32562425479161305 1104 6 0.2452635359929758 1104 7 0.27669103489790103 1104 8 0.2907810444762049 1104 9 0.4830016378847404 1104 10-11 0.3776741246245692 1104 12-13 0.39203415457013335 1104 14-15 0.46047214179897367 1104 16-17 0.496732634828966 1104 18-19 0.48935347510745686 1104 20-21 0.47332285078704217 1104 22-23 0.5145045246692632 1104 24-25 0.5037615914378151 1104 26-27 0.5732318920981143 1104 28-29 0.5679820443995354 1104 30-31 0.47695078639949173 1104 32-33 0.5036295498568251 1104 34-35 0.42050308312282425 1104 36-37 0.413957661719877 1104 38-39 0.471419829712616 1104 40-41 0.4659151877978758 1104 42-43 0.42656742998548935 1104 44-45 0.4646126281193972 1104 46-47 0.4198058727088778 1104 48-49 0.46273073778977647 1104 50-51 0.4356334456579489 1104 52-53 0.38850924764262373 1104 54-55 0.5259561154433001 1104 56-57 0.5085647501372748 1104 58-59 0.4979676050629749 1104 60-61 0.48580497647650844 1104 62-63 0.515184632710934 1104 64-65 0.4513239804522726 1104 66-67 0.49970665710036855 1104 68-69 0.5194015529006535 1104 70-71 0.4244895639386712 1104 72-73 0.3777375412153887 1104 74-75 0.3647927434558653 1104 76-77 0.42990106126456595 1104 78-79 0.4111143344409349 1104 80-81 0.46632467219389184 1104 82-83 0.5137354289469158 1104 84-85 0.4518934040383584 1104 86-87 0.4201734085082123 1104 88-89 0.40908522615276155 1104 90-91 0.3372696940726847 1104 92-93 0.34593452981848927 1104 94-95 0.36613459841771956 1104 96-97 0.4633391460242464 1104 98-99 0.44279520670487926 1104 100-101 0.48337019643489754 1104 102-103 0.33791145944947587 1104 104-105 0.4899503362179338 1104 106-107 0.4939334249850198 1104 108-109 0.5297218730551769 1104 110-111 0.5183154850601603 1104 112-113 0.49826016481353363 1104 114-115 0.5684124100321633 1104 116-117 0.538252233569473 1104 118-119 0.4887271314403634 1104 120-121 0.5305535033652653 1104 122-123 0.4879986426481189 1104 124-125 0.5398151253634005 1105 1 -0.012313589480900333 1105 2 0.08935024031595162 1105 3 0.06408687161572146 1105 4 0.04774740487242468 1105 5 -0.034568924063819395 1105 6 -0.04024959458047306 1105 7 0.0776812351575984 1105 8 0.017530365140601134 1105 9 0.025925864620909067 1105 10-11 0.03034503928305554 1105 12-13 0.03721733170754504 1105 14-15 0.05497454933699686 1105 16-17 0.04531821766068589 1105 18-19 0.05697640039944929 1105 20-21 -0.0013628006052641695 1105 22-23 0.02330337339618893 1105 24-25 0.06994581295733582 1105 26-27 0.14729839426292557 1105 28-29 0.1850019792362474 1105 30-31 0.0732905379702018 1105 32-33 -0.008036851141952184 1105 34-35 -0.004722634467015041 1105 36-37 0.05891654834420734 1105 38-39 0.05482722062484413 1105 40-41 0.033254331054628494 1105 42-43 0.0281299226321039 1105 44-45 -0.03947451419855241 1105 46-47 -0.014425128359313533 1105 48-49 -0.05024306484575192 1105 50-51 -0.09637947117234802 1105 52-53 -0.037378351195933135 1105 54-55 0.04131649980337926 1105 56-57 -0.020494297731495692 1105 58-59 -0.04110157657341773 1105 60-61 0.0031434193444610514 1105 62-63 0.056014863063545306 1105 64-65 0.12787300376426458 1105 66-67 0.16943652351103822 1105 68-69 0.10029546160549785 1105 70-71 0.06276948215319322 1105 72-73 0.07870712845164007 1105 74-75 0.03150039071039856 1105 76-77 -0.017667750826767303 1105 78-79 -0.007171360735355847 1105 80-81 -0.05602946796481234 1105 82-83 -0.041666542450975896 1105 84-85 -0.025111780001392248 1105 86-87 -0.034464181264052485 1105 88-89 -0.12121273474140537 1105 90-91 -0.07109262695929175 1105 92-93 -0.07451666783804001 1105 94-95 -0.04633706698003337 1105 96-97 -0.01049870273130793 1105 98-99 -0.1209419449672744 1105 100-101 -0.1103685658546425 1105 102-103 -0.2673850466845771 1105 104-105 -0.11952940114678867 1105 106-107 -0.05867997474258857 1105 108-109 -0.0259393806119661 1105 110-111 -0.08555369515884337 1105 112-113 -0.07245548913062194 1105 114-115 -0.10708173917457131 1105 116-117 -0.11844652566825431 1105 118-119 -0.16425826609007288 1105 120-121 -0.1522430710082432 1105 122-123 -0.05169931094870961 1105 124-125 0.03766404590924921 1106 1 -0.12102738290329285 1106 2 -0.09465455207431361 1106 3 -0.12322154947148789 1106 4 -0.12189275093212615 1106 5 -0.2329478617506453 1106 6 -0.11244604843643202 1106 7 -0.06930890128375466 1106 8 -0.08723369348837906 1106 9 -0.036702502975479945 1106 10-11 -0.006730991072210202 1106 12-13 -0.039472663356569626 1106 14-15 -0.06832325635508596 1106 16-17 -0.04140168446010506 1106 18-19 0.02521879191802867 1106 20-21 -0.06729209556028337 1106 22-23 -0.005535709177451054 1106 24-25 0.013426846104700019 1106 26-27 0.09246568382219067 1106 28-29 0.23859139872081414 1106 30-31 0.10022885085692934 1106 32-33 -0.10994292085462831 1106 34-35 0.06768654978068867 1106 36-37 0.027705717749675784 1106 38-39 -0.011116121289376224 1106 40-41 -0.03041248501406102 1106 42-43 -0.03276116027175391 1106 44-45 -0.02272616266323979 1106 46-47 -0.03941253472012107 1106 48-49 -0.12683378572705095 1106 50-51 -0.16419451637329985 1106 52-53 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106-107 -0.7113923783263054 2216 108-109 -0.7381197572338145 2216 110-111 -0.7384783512234385 2216 112-113 -0.8039330198252053 2216 114-115 -0.8211346192273652 2216 116-117 -0.7486941746361673 2216 118-119 -0.7804366739332274 2216 120-121 -0.7941073263225 2216 122-123 -0.8256089711261971 2216 124-125 -1.040845773268206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62092.0 3 10169.0 4 6254.0 5 4796.0 6 5389.0 7 5713.0 8 6467.0 9 7055.0 10 8615.0 11 10017.0 12 11304.0 13 12112.0 14 12561.0 15 12288.0 16 11812.0 17 11638.0 18 11944.0 19 12418.0 20 12967.0 21 14172.0 22 15971.0 23 19137.0 24 23504.0 25 26518.0 26 30968.0 27 34610.0 28 40490.0 29 50067.0 30 59722.0 31 70867.0 32 90102.0 33 115878.0 34 154143.0 35 220312.0 36 398629.0 37 776058.0 38 386062.0 39 2757.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.16061248825534 18.341366573726688 11.352047767805564 24.145973170212407 2 15.18539858115629 21.429894821888904 38.16713554578401 25.217571051170793 3 20.64936914109039 26.551321578222435 28.390467085495768 24.408842195191408 4 13.230292787270745 16.818114620984197 35.33377574515335 34.6178168465917 5 12.997051201858637 39.12860435904869 34.009835139009056 13.864509300083618 6 32.31444744182059 33.45093574563586 18.654973267005186 15.57964354553836 7 27.454313395181053 32.64105151288639 21.464804406776953 18.439830685155602 8 27.129940735874996 34.06579073572967 21.143363086501207 17.660905441894126 9 28.24419020833674 15.684429885521078 19.795169756198593 36.27621014994359 10-11 24.737162358215752 27.56099205050698 26.611351679081256 21.090493912196013 12-13 25.746442617297603 25.282372191676238 26.496964156001212 22.474221035024943 14-15 24.66781174254974 25.756233252538642 24.27012311018885 25.30583189472277 16-17 23.092782194305208 28.92099385944756 26.853913705302528 21.132310240944708 18-19 23.478580521261105 28.001524883885615 27.748618641542045 20.77127595331124 20-21 23.01876654955813 27.857240682569255 27.439544275710144 21.68444849216247 22-23 22.356225156419182 28.411409698352603 25.424057669808377 23.808307475419845 24-25 22.5437693361796 29.06065220961036 25.050261351763343 23.345317102446693 26-27 23.18819964724363 27.574274036364532 25.421584555164188 23.815941761227645 28-29 22.957382220123893 27.342831643538524 26.77926192785385 22.920524208483737 30-31 23.91827119608525 28.15174707754538 26.024480633185483 21.905501093183886 32-33 21.667876934558876 28.39141317201434 26.99681030672252 22.943899586704262 34-35 21.20436677240307 28.871524727912394 27.157366907381846 22.766741592302687 36-37 23.439591950060404 27.986754247750987 26.07058360373294 22.503070198455667 38-39 24.13407725907726 27.305708755176838 26.072288439309716 22.487925546436184 40-41 22.597772724698668 28.53303523070646 26.024791306380674 22.8444007382142 42-43 23.610355536604928 28.00307058218536 25.818266769261434 22.56830711194828 44-45 24.342830164696483 27.251747743821614 25.799477038731816 22.605945052750087 46-47 22.737970497596553 28.087301399938617 27.541672190271072 21.633055912193758 48-49 23.569954948730096 27.657401154237903 27.309560824117362 21.463083072914632 50-51 21.90804014598739 28.161745254147952 27.397437613014308 22.53277698685035 52-53 21.62946924202149 28.7445444795916 25.592393399144836 24.03359287924207 54-55 22.10911624829742 28.908746736908693 25.152465206704512 23.82967180808938 56-57 22.873812723867946 27.662272265534522 25.512885862473833 23.951029148123702 58-59 22.931007471756494 27.295156619336524 26.51450610689337 23.25932980201361 60-61 23.550621428738715 28.128934594255334 26.12054067214194 22.199903304864005 62-63 21.4860000646338 28.409823320964556 26.883212895387288 23.220963719014357 64-65 20.80328969958757 28.59439101571799 27.269798837107324 23.332520447587118 66-67 22.882987992739686 28.171317112919592 26.031950246116793 22.91374464822393 68-69 23.679818580929574 27.25961337693298 26.01317430719594 23.047393734941508 70-71 22.031313043639315 28.38565792727858 26.029534974681766 23.55349405440034 72-73 23.271299752627122 27.80617863833606 25.939287871901417 22.983233737135404 74-75 23.89896688256833 27.119968155896647 25.831331575999833 23.149733385535193 76-77 22.48242858084451 27.74420364234833 27.626071266905267 22.147296509901892 78-79 23.21065030207024 27.611189534888997 27.241461012287065 21.9366991507537 80-81 21.70851583293356 28.12802892442383 27.247745635897548 22.91570960674506 82-83 21.428016337119594 28.477983853894116 25.658116684684657 24.43588312430163 84-85 21.947048745396323 28.622550546921353 25.190374823242834 24.240025884439486 86-87 22.615334527281377 27.107946860293737 25.79842929798143 24.47828931444346 88-89 22.751332357523893 26.787603129029435 26.696443258109344 23.764621255337328 90-91 23.333766060455307 27.813090933519497 26.149094768147528 22.704048237877664 92-93 21.58590284372668 27.71670228663879 26.931132097740964 23.766262771893572 94-95 20.946225914564728 27.861427242820263 27.364729542609602 23.82761730000541 96-97 22.967470967760672 27.293336344137863 26.408700741035474 23.33049194706599 98-99 23.53237824970212 26.61760590805109 26.12068924843147 23.729326593815316 100-101 22.152572465951 27.51092986810119 26.17758758230519 24.158910083642617 102-103 23.12288153890312 27.07479009069127 26.155376613894965 23.646951756510646 104-105 23.66006013590453 26.31736894168501 26.275293403847506 23.74727751856295 106-107 22.23897419834747 27.06216412746592 27.733124324806944 22.965737349379662 108-109 22.796211145585453 27.09914381995392 27.28576624266614 22.818878791794496 110-111 21.60571677924371 27.583318267395352 27.367766443836 23.44319850952494 112-113 21.37022772390267 27.909078879947113 25.89007372380066 24.830619672349556 114-115 21.606588518699553 28.522888709843407 25.483009868656893 24.387512902800147 116-117 22.256884349746997 27.154980477149426 25.791901183419714 24.796233989683863 118-119 22.279018296531802 27.052412673992833 26.46405304243671 24.204515987038654 120-121 22.7480242019036 27.81528187822811 26.147056147551922 23.28963777231636 122-123 21.466977664430992 27.920949692783825 26.57455324324079 24.037519399544394 124-125 21.007968507858696 28.285897763074928 26.87343064755425 23.832703081512125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11294.0 1 6287.0 2 1719.5 3 2245.5 4 2202.5 5 1922.0 6 1581.5 7 1376.0 8 1335.0 9 1255.0 10 1164.5 11 1091.0 12 1039.5 13 1102.0 14 1204.0 15 1301.5 16 1455.0 17 1700.5 18 2014.0 19 2360.0 20 2943.0 21 3812.5 22 4940.0 23 6522.5 24 8433.5 25 10546.5 26 13806.0 27 18025.0 28 21135.5 29 24303.5 30 28588.5 31 32815.5 32 37101.0 33 42121.0 34 48356.0 35 53303.5 36 58183.0 37 63296.5 38 65694.5 39 67309.5 40 68992.5 41 72624.5 42 76553.5 43 79899.5 44 83928.5 45 87489.0 46 91040.5 47 95916.5 48 104950.0 49 127417.5 50 198833.0 51 206020.5 52 129628.0 53 93638.5 54 82175.5 55 73829.0 56 65617.5 57 58359.0 58 54048.0 59 49450.5 60 43080.0 61 35884.5 62 29429.0 63 24042.5 64 18925.5 65 14624.0 66 10807.5 67 8507.5 68 6574.5 69 4578.0 70 3491.5 71 2949.5 72 2546.0 73 2304.5 74 1827.5 75 1419.5 76 1019.0 77 568.0 78 358.5 79 263.0 80 182.5 81 115.0 82 81.5 83 60.5 84 45.5 85 37.5 86 32.0 87 30.0 88 27.0 89 25.5 90 19.0 91 12.0 92 11.5 93 9.5 94 7.5 95 8.0 96 6.5 97 4.0 98 3.5 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47961041055432174 2 0.4669548282492846 3 0.45220203516226987 4 0.4515150178371393 5 0.4553116925286504 6 0.46283272429850103 7 0.4470674846270834 8 0.4756329418298815 9 0.48969871759176564 10-11 0.4932603600404689 12-13 0.49266373973180294 14-15 0.46724409870197114 16-17 0.46917859485431257 18-19 0.45493202505949937 20-21 0.4650384115002361 22-23 0.48300933837338883 24-25 0.46883508619174724 26-27 0.4792307430851706 28-29 0.474530098229014 30-31 0.4890478590732209 32-33 0.48570316946403247 34-35 0.4806770953486034 36-37 0.43721420983244735 38-39 0.39781919005719596 40-41 0.39131060487174835 42-43 0.3996090509831941 44-45 0.43491812561424775 46-47 0.4426922690301991 48-49 0.43967300868028314 50-51 0.42589650337108553 52-53 0.41949639460539534 54-55 0.4220094316631099 56-57 0.40367691672409894 58-59 0.4076001472386604 60-61 0.4180500423419625 62-63 0.4194783152021024 64-65 0.4343938229187533 66-67 0.4231484340705632 68-69 0.42425127767143067 70-71 0.43495428442083356 72-73 0.4354424283097421 74-75 0.4398357233099193 76-77 0.42983781328893994 78-79 0.42181055822688784 80-81 0.43097681569639334 82-83 0.4284456992353859 84-85 0.42667391771268065 86-87 0.43276667662239143 88-89 0.42034612656016207 90-91 0.42398008662203707 92-93 0.4329293912520276 94-95 0.4299643691119903 96-97 0.3996994479996587 98-99 0.37196564334833443 100-101 0.37185716692857695 102-103 0.35236757017881976 104-105 0.3550794806727563 106-107 0.37415325114677656 108-109 0.3814754094804052 110-111 0.39847004857574075 112-113 0.3942937064150785 114-115 0.39219649563310094 116-117 0.36480619964434197 118-119 0.439691088083576 120-121 0.4456030529603577 122-123 0.39785534886378177 124-125 0.3926484807154237 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2765578.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.53706308446828 #Duplication Level Percentage of deduplicated Percentage of total 1 84.17582257491958 50.957570814049156 2 10.225565728661104 12.380514351806681 3 2.763533365185545 5.018885809928108 4 1.0407564581196727 2.520173574430336 5 0.5025642327214881 1.5211881330129062 6 0.28153046172005697 1.0225816392807943 7 0.18933355003859662 0.8023187943885 8 0.12265827164092094 0.5940297222526622 9 0.09728680624860071 0.5300511774442186 >10 0.5199115062397687 6.004113933041416 >50 0.049813152521755236 2.1011500631012145 >100 0.02774124320889749 2.9469674031373883 >500 9.607306962772728E-4 0.3822553825608555 >1k 0.0013210047073812501 2.571536209954846 >5k 6.004566851732956E-4 2.5496535796484907 >10k+ 6.004566851732956E-4 8.097009411962313 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 50540 1.827466084847363 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 25963 0.938791095387655 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 12713 0.4596869081255347 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 12066 0.4362921602645089 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 10094 0.36498699367727105 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 9496 0.3433640273389505 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 8639 0.31237593009490244 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 6176 0.22331678947402678 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 5739 0.20751539099602329 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 5021 0.18155336786740423 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 4995 0.18061323889617287 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 4816 0.17414081251731103 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 4200 0.15186698766044565 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 4022 0.14543072008816965 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 3556 0.12858071621917733 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 3487 0.1260857585647557 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 3375 0.12203597222714385 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.6158806585820394E-5 0.0 5 0.0 0.0 0.0 3.6158806585820394E-5 0.0 6 7.231761317164079E-5 0.0 0.0 7.231761317164079E-5 0.0 7 2.5311164610074277E-4 0.0 0.0 7.231761317164079E-5 0.0 8 2.5311164610074277E-4 0.0 0.0 7.231761317164079E-5 0.0 9 2.5311164610074277E-4 0.0 0.0 1.4463522634328157E-4 0.0 10-11 2.5311164610074277E-4 0.0 0.0 2.5311164610074277E-4 0.0 12-13 2.5311164610074277E-4 0.0 0.0 3.977468724440244E-4 0.0 14-15 2.5311164610074277E-4 0.0 0.0 6.689379218376772E-4 0.0 16-17 2.5311164610074277E-4 0.0 0.0 0.0014101934568469955 0.0 18-19 2.5311164610074277E-4 0.0 0.0 0.0020610519753917625 0.0 20-21 2.5311164610074277E-4 0.0 0.0 0.003181974979552195 0.0 22-23 2.5311164610074277E-4 0.0 0.0 0.00462832724298501 0.0 24-25 2.5311164610074277E-4 0.0 0.0 0.006201235329468198 0.0 26-27 2.5311164610074277E-4 0.0 0.0 0.008551557757546523 0.0 28-29 2.8927045268656315E-4 0.0 0.0 0.012836376337966242 0.0 30-31 2.8927045268656315E-4 0.0 0.0 0.024172162202620938 0.0 32-33 3.0734985597947334E-4 0.0 0.0 0.04664486049570832 0.0 34-35 3.6158806585820396E-4 0.0 0.0 0.08343644619678056 0.0 36-37 3.6158806585820396E-4 0.0 0.0 0.14031424895627603 0.0 38-39 3.6158806585820396E-4 0.0 0.0 0.21836303297176937 0.0 40-41 3.6158806585820396E-4 0.0 0.0 0.3087600494363204 0.0 42-43 3.6158806585820396E-4 0.0 0.0 0.38888796483049837 0.0 44-45 3.9774687244402434E-4 0.0 0.0 0.487601506809788 0.0 46-47 3.9774687244402434E-4 0.0 0.0 0.6162906994487228 0.0 48-49 3.9774687244402434E-4 0.0 0.0 0.7422499021904282 0.0 50-51 3.9774687244402434E-4 0.0 0.0 0.8644124302406224 0.0 52-53 3.9774687244402434E-4 0.0 0.0 0.9943671811100608 0.0 54-55 3.9774687244402434E-4 0.0 0.0 1.1385504223710197 0.0 56-57 4.7006448561566516E-4 0.0 0.0 1.3147522868637225 0.0 58-59 4.7006448561566516E-4 0.0 0.0 1.513191817406705 0.0 60-61 4.7006448561566516E-4 0.0 0.0 1.7051408421675325 0.0 62-63 4.7006448561566516E-4 0.0 0.0 1.8904004877099831 0.0 64-65 4.7006448561566516E-4 0.0 0.0 2.1006097097966503 0.0 66-67 4.7006448561566516E-4 0.0 0.0 2.3592536533050232 0.0 68-69 4.7006448561566516E-4 0.0 0.0 2.6591728745311105 0.0 70-71 4.7006448561566516E-4 0.0 0.0 3.0125528912943333 0.0 72-73 4.7006448561566516E-4 0.0 0.0 3.333986602438984 0.0 74-75 4.8814388890857535E-4 0.0 0.0 3.6499060955792966 0.0 76-77 5.062232922014855E-4 0.0 0.0 4.020895451149814 0.0 78-79 5.062232922014855E-4 0.0 0.0 4.417051336104063 0.0 80-81 6.870173251305875E-4 0.0 0.0 4.836674286532508 0.0 82-83 7.231761317164079E-4 0.0 0.0 5.291479755768957 0.0 84-85 7.412555350093181E-4 0.0 0.0 5.745833239923083 0.0 86-87 7.593349383022283E-4 0.0 0.0 6.225895635559727 0.0 88-89 7.593349383022283E-4 0.0 0.0 6.763884439346857 0.0 90-91 7.593349383022283E-4 0.0 0.0 7.3199707258301885 0.0 92-93 7.593349383022283E-4 0.0 0.0 7.878298858321841 0.0 94-95 7.593349383022283E-4 0.0 0.0 8.464143842625303 0.0 96-97 7.593349383022283E-4 0.0 0.0 9.068050150818383 0.0 98-99 8.497319547667794E-4 0.0 0.0 9.732630936462469 3.6158806585820394E-5 100-101 9.039701646455099E-4 0.0 0.0 10.53069195661811 3.6158806585820394E-5 102-103 9.039701646455099E-4 0.0 0.0 11.279992826092773 3.6158806585820394E-5 104-105 0.0010486053909887915 0.0 0.0 11.99608183171836 3.6158806585820394E-5 106-107 0.0010486053909887915 0.0 0.0 12.744695683867892 3.6158806585820394E-5 108-109 0.001102843600867522 0.0 0.0 13.488174985482239 3.6158806585820394E-5 110-111 0.0011570818107462526 0.0 0.0 14.272115991666118 3.6158806585820394E-5 112-113 0.0011570818107462526 0.0 0.0 15.125590382914531 3.6158806585820394E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 9590 0.0 25.888288 4 ATGGGGG 4390 0.0 22.921322 5 ATGGGGA 5105 0.0 21.343853 5 CATGGGT 5050 0.0 18.391584 4 GTGTAGC 1280 0.0 18.139147 1 AGTACTC 1765 0.0 16.529848 5 ATTCGCG 370 0.0 16.079935 80-81 ATGGGAT 3370 0.0 16.077887 5 TATACAG 1635 0.0 16.023315 5 GTATAGG 980 0.0 15.794631 1 CCGTCTA 265 4.220362E-4 15.725001 9 ATGGGTA 2835 0.0 15.541614 5 ATGGGAG 3260 0.0 15.524543 5 GTACTAT 835 0.0 14.972525 1 TGGGGAG 3425 0.0 14.94777 6 TACATGG 89670 0.0 14.751741 2 GTAATAC 525 1.4186298E-8 14.742458 3 GTACATG 90600 0.0 14.679699 1 ACATGGG 88645 0.0 14.372934 3 CTCGCGC 415 0.0 14.334114 60-61 >>END_MODULE