FastQCFastQC Report
Thu 26 May 2016
SRR1364165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2765578
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA467101.6889778556236708No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT404691.4633107437215656No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG263310.9520975362112369No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC136110.49215751643960143No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG133530.48282854434045974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116370.42078003223919197No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG110270.3987231602218415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109810.3970598551188938No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT86040.31111037186439866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70130.2535817105863585No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC68600.24804941317872792No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT67200.24298718025671304No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA58250.2106250483624038No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG55040.19901807144835545No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG53390.1930518683616951No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC50650.1831443553571803No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG44320.160255830788356No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43240.1563506796770874No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG41970.1517585112406882No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA39090.14134477494397193No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGA1358.247354E-526.4458358
GTTATCA15650.024.7437361
GAGTACT161850.022.07620412-13
AGTACTC17700.021.5125315
GCGCGAC1450.00386674320.5190629
AGTACTT165750.020.46187212-13
GTACTTT175450.020.39740414-15
CATGGGG74150.020.2999134
TTATCAA21000.018.4153042
ACTTTTT200550.017.93290516-17
GTGTAGC13400.017.7836741
ATGGGCG4501.8590072E-917.1876165
TACTTTT199200.016.9051814-15
AAAGTAC20500.016.2524553
GTATAGG9900.016.247811
AAGTACT23450.016.2376044
TAAAGCG2603.667139E-416.0180475
CGTACGC1150.002993414915.5203178-79
TAACGCA2350.003453393215.1903654
GTCTAGA8300.015.0732711
Produced by FastQC (version 0.11.5)