##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364165_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2765578 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.050346437525896 33.0 31.0 34.0 30.0 34.0 2 31.88659513490489 33.0 31.0 34.0 30.0 34.0 3 32.109138487506044 34.0 31.0 34.0 30.0 34.0 4 35.605035186134685 37.0 35.0 37.0 33.0 37.0 5 35.733949648138655 37.0 35.0 37.0 33.0 37.0 6 35.843180702189564 37.0 35.0 37.0 35.0 37.0 7 35.82079731614874 37.0 35.0 37.0 35.0 37.0 8 35.858817216509536 37.0 35.0 37.0 35.0 37.0 9 37.529597791130826 39.0 37.0 39.0 35.0 39.0 10-11 37.486691751236094 39.0 37.0 39.0 34.0 39.0 12-13 37.463336778062306 39.0 37.0 39.0 34.0 39.0 14-15 38.76532518699527 40.0 38.0 41.0 34.0 41.0 16-17 38.75417905407116 40.0 38.0 41.0 34.0 41.0 18-19 38.85665058081891 40.0 38.0 41.0 34.5 41.0 20-21 38.91712166498287 40.0 38.0 41.0 35.0 41.0 22-23 38.8869975824222 40.0 38.0 41.0 35.0 41.0 24-25 38.85711847577613 40.0 38.0 41.0 35.0 41.0 26-27 38.71556596848832 40.0 38.0 41.0 34.0 41.0 28-29 38.59129104295739 40.0 38.0 41.0 34.0 41.0 30-31 38.495975705621035 40.0 38.0 41.0 34.0 41.0 32-33 38.31702559103377 40.0 38.0 41.0 34.0 41.0 34-35 38.172100371061674 40.0 38.0 41.0 34.0 41.0 36-37 38.07002279451167 40.0 38.0 41.0 33.0 41.0 38-39 37.989492250806165 40.0 37.5 41.0 33.0 41.0 40-41 37.83856448814678 40.0 37.0 41.0 33.0 41.0 42-43 37.70458255019385 40.0 37.0 41.0 33.0 41.0 44-45 37.493485267817434 40.0 37.0 41.0 33.0 41.0 46-47 37.20166381132624 40.0 37.0 41.0 32.0 41.0 48-49 36.90082362529641 40.0 36.5 41.0 31.0 41.0 50-51 36.70317796135202 40.0 36.0 41.0 31.0 41.0 52-53 36.562515864676385 40.0 36.0 41.0 31.0 41.0 54-55 36.393430053319776 39.5 35.5 41.0 30.0 41.0 56-57 36.17704129118759 39.0 35.0 41.0 30.0 41.0 58-59 36.031359448187686 39.0 35.0 41.0 30.0 41.0 60-61 35.89187847892917 39.0 35.0 41.0 30.0 41.0 62-63 35.6154971944382 38.5 35.0 40.5 29.0 41.0 64-65 35.33137430945719 38.0 35.0 40.0 29.0 41.0 66-67 34.97930342228641 37.0 35.0 40.0 29.0 41.0 68-69 34.67011398702188 37.0 34.5 39.5 28.5 41.0 70-71 34.35025589587421 36.0 34.0 39.0 28.5 41.0 72-73 34.0932072789124 36.0 34.0 39.0 29.0 40.5 74-75 33.74063288035991 35.0 34.0 38.0 28.0 40.0 76-77 33.35165885756974 35.0 34.0 37.0 27.5 39.0 78-79 33.040428619261505 35.0 34.0 37.0 27.0 39.0 80-81 32.80500531896045 35.0 34.0 36.0 27.0 38.5 82-83 32.570247340700575 35.0 34.0 36.0 27.0 37.0 84-85 32.3551966352061 35.0 34.0 35.5 27.0 37.0 86-87 32.14242411532056 35.0 33.0 35.0 26.0 36.5 88-89 31.972570833294164 35.0 33.0 35.0 26.0 36.0 90-91 31.84810571244058 35.0 33.0 35.0 26.0 36.0 92-93 31.718867448323643 35.0 33.0 35.0 25.0 36.0 94-95 31.643015131014202 35.0 33.0 35.0 25.0 35.5 96-97 31.580230606404882 35.0 33.0 35.0 25.0 35.0 98-99 31.513819353495002 35.0 33.0 35.0 25.0 35.0 100-101 31.40792557649793 35.0 33.0 35.0 24.5 35.0 102-103 30.24941115383475 34.0 31.0 35.0 21.5 35.0 104-105 31.076751948417293 35.0 32.5 35.0 23.5 35.0 106-107 31.164035510840773 35.0 33.0 35.0 23.5 35.0 108-109 31.171275227095386 35.0 33.0 35.0 23.5 35.0 110-111 31.16469034682804 35.0 33.0 35.0 23.5 35.0 112-113 31.098962314568602 35.0 33.0 35.0 23.0 35.0 114-115 31.021241310134805 35.0 33.0 35.0 20.0 35.0 116-117 30.89414274339758 35.0 33.0 35.0 19.0 35.0 118-119 30.775676549350624 35.0 33.0 35.0 18.0 35.0 120-121 30.6519463562409 35.0 33.0 35.0 11.0 35.0 122-123 30.452648054041507 35.0 33.0 35.0 2.0 35.0 124-125 29.536407217587065 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3674211829850833 1101 2 0.7156324159823733 1101 3 0.5586394045459784 1101 4 0.5600140668713749 1101 5 0.2659103758462962 1101 6 0.22777089638737635 1101 7 0.23013408716822426 1101 8 0.24054751476401037 1101 9 0.37149014379983925 1101 10-11 0.3330182860697306 1101 12-13 0.3086296019634176 1101 14-15 0.42498919956244663 1101 16-17 0.5065811385361769 1101 18-19 0.3993714672538218 1101 20-21 0.35234997811836166 1101 22-23 0.3552891260416047 1101 24-25 0.3519419065048126 1101 26-27 0.3739292148512945 1101 28-29 0.4462268378841969 1101 30-31 0.3980009928498305 1101 32-33 0.38657223018044107 1101 34-35 0.47692300566947665 1101 36-37 0.4765559272730471 1101 38-39 0.44224590636904537 1101 40-41 0.4779090238962027 1101 42-43 0.5031381913143775 1101 44-45 0.5482990376023551 1101 46-47 0.5718755740058796 1101 48-49 0.5348822253732806 1101 50-51 0.6377228896963842 1101 52-53 0.6211508134589678 1101 54-55 0.5884500963835109 1101 56-57 0.5720173963372446 1101 58-59 0.556186683449944 1101 60-61 0.5548217758801854 1101 62-63 0.5459624668185086 1101 64-65 0.5674008709415972 1101 66-67 0.6193004684593717 1101 68-69 0.6484068920857169 1101 70-71 0.5983672415452972 1101 72-73 0.6500360993054244 1101 74-75 0.6258983523012702 1101 76-77 0.5538306683054728 1101 78-79 0.4622068774123491 1101 80-81 0.43267456195236775 1101 82-83 0.4867507129154305 1101 84-85 0.45135472061319604 1101 86-87 0.45796931582140843 1101 88-89 0.4585135993095335 1101 90-91 0.481776511892793 1101 92-93 0.4115663911556844 1101 94-95 0.459612198942299 1101 96-97 0.45780776963130876 1101 98-99 0.46447688794880193 1101 100-101 0.4341164497020493 1101 102-103 0.3708829676738361 1101 104-105 0.47459570445134247 1101 106-107 0.4618464366994104 1101 108-109 0.44612307106222815 1101 110-111 0.44802174931682615 1101 112-113 0.5281530999353521 1101 114-115 0.4988411278813203 1101 116-117 0.46101593965680365 1101 118-119 0.48671776676630785 1101 120-121 0.5453868352871005 1101 122-123 0.45371096142397604 1101 124-125 0.5502893516293206 1102 1 0.3048669915163771 1102 2 0.696433565328828 1102 3 0.4291211024791508 1102 4 0.4767421595602954 1102 5 0.32213654393340363 1102 6 0.2698967541557593 1102 7 0.24842640765202617 1102 8 0.24815206835144465 1102 9 0.3606057655021786 1102 10-11 0.23856136791133054 1102 12-13 0.23957375782788404 1102 14-15 0.376656004603106 1102 16-17 0.3785190122211688 1102 18-19 0.34350501139692824 1102 20-21 0.28365011613137625 1102 22-23 0.2545596544257265 1102 24-25 0.36838885932890264 1102 26-27 0.3426051321204895 1102 28-29 0.47337172508532177 1102 30-31 0.4077659856724267 1102 32-33 0.40628974267320217 1102 34-35 0.46500731995412536 1102 36-37 0.39962148887294546 1102 38-39 0.406784922421636 1102 40-41 0.3523025547313452 1102 42-43 0.32641636756916625 1102 44-45 0.3569457278768269 1102 46-47 0.3644548773469509 1102 48-49 0.3035923751661329 1102 50-51 0.2739482997346343 1102 52-53 0.3181651237367902 1102 54-55 0.2673812715637851 1102 56-57 0.2629945092781014 1102 58-59 0.258289787720166 1102 60-61 0.24466037163679744 1102 62-63 0.26123727156075915 1102 64-65 0.27549972051221516 1102 66-67 0.24946070554817368 1102 68-69 0.2634017000526825 1102 70-71 0.19631804297138444 1102 72-73 0.22785053616536288 1102 74-75 0.22052992250922898 1102 76-77 0.14632267570306823 1102 78-79 0.1505571104221346 1102 80-81 0.09896767898909786 1102 82-83 0.12422106779059305 1102 84-85 0.12650846615665046 1102 86-87 0.16939744376561805 1102 88-89 0.17153796155580636 1102 90-91 0.15490867349918247 1102 92-93 0.15956941372508737 1102 94-95 0.12135166838029576 1102 96-97 0.1254221778538529 1102 98-99 0.15019837598667252 1102 100-101 0.12339535272112911 1102 102-103 0.05667003295387474 1102 104-105 0.086240417782534 1102 106-107 0.15999108204685442 1102 108-109 0.12414759574754797 1102 110-111 0.1252530300223178 1102 112-113 0.1011354566490752 1102 114-115 0.1286520608710049 1102 116-117 0.14111565316246555 1102 118-119 0.15531878158916967 1102 120-121 0.12462869017248579 1102 122-123 0.15528878596815332 1102 124-125 0.21377744725417713 1103 1 0.2762129934673325 1103 2 0.5505816820030809 1103 3 0.40302990370734193 1103 4 0.5474525553869185 1103 5 0.239155479185591 1103 6 0.22058531537242487 1103 7 0.22480459353769078 1103 8 0.08346451064706883 1103 9 0.2993078896031989 1103 10-11 0.2644419910363993 1103 12-13 0.22480590947232315 1103 14-15 0.3836735815366339 1103 16-17 0.39667051212401105 1103 18-19 0.3005226850079481 1103 20-21 0.17601526554160074 1103 22-23 0.20603662053156313 1103 24-25 0.13089735486288845 1103 26-27 0.18535267723745363 1103 28-29 0.22828523740734852 1103 30-31 0.1985876911212685 1103 32-33 0.16981667508694187 1103 34-35 0.24787554999240058 1103 36-37 0.2771746808251194 1103 38-39 0.2370053011653397 1103 40-41 0.3057172397512673 1103 42-43 0.26232124949211766 1103 44-45 0.2526855861669688 1103 46-47 0.23922025317583717 1103 48-49 0.2479453586816618 1103 50-51 0.2929792626327128 1103 52-53 0.30323485720663257 1103 54-55 0.3043543671865194 1103 56-57 0.28436820932846985 1103 58-59 0.21894676440252425 1103 60-61 0.14522286541318863 1103 62-63 0.172948854586906 1103 64-65 0.189629388705427 1103 66-67 0.20830516065936422 1103 68-69 0.2551059259222228 1103 70-71 0.2127264478218862 1103 72-73 0.2195272693751491 1103 74-75 0.2948002250227475 1103 76-77 0.28118196645770155 1103 78-79 0.16947701505946355 1103 80-81 0.1531107563494487 1103 82-83 0.09972824996093976 1103 84-85 0.07843848712600021 1103 86-87 0.06852832526125496 1103 88-89 0.13733008369427324 1103 90-91 0.1243909752354142 1103 92-93 0.06137596546156132 1103 94-95 0.08808941716771557 1103 96-97 0.08363895439448044 1103 98-99 0.04478541569308092 1103 100-101 -0.03601530616493065 1103 102-103 -0.09999558959595589 1103 104-105 0.1120442427305477 1103 106-107 0.14414135221208824 1103 108-109 0.10031919393271949 1103 110-111 0.15145564959544444 1103 112-113 0.23528132798874424 1103 114-115 0.16633724659328664 1103 116-117 0.1645333569368539 1103 118-119 0.11574252383925909 1103 120-121 0.055169240576741885 1103 122-123 0.030275432390553192 1103 124-125 0.11690594274949007 1104 1 0.2631346050237582 1104 2 0.5738347729506366 1104 3 0.0542444423072439 1104 4 0.446285815184261 1104 5 0.21821436458402843 1104 6 0.20419774912623012 1104 7 0.18024810142274816 1104 8 0.1492139839483997 1104 9 0.2750282523931489 1104 10-11 0.266307618966529 1104 12-13 0.21910112842633112 1104 14-15 0.3181424544687559 1104 16-17 0.38206697668963585 1104 18-19 0.29651218954235503 1104 20-21 0.24329348144235752 1104 22-23 0.18651101585458463 1104 24-25 0.17845676011674527 1104 26-27 0.20295526923051455 1104 28-29 0.29129613279741307 1104 30-31 0.349757574581794 1104 32-33 0.27697309032544837 1104 34-35 0.2625855038433471 1104 36-37 0.3021354828917353 1104 38-39 0.2592228858256931 1104 40-41 0.22586251277859049 1104 42-43 0.2827978346567761 1104 44-45 0.32727300747534116 1104 46-47 0.34337752486513295 1104 48-49 0.33026238163834876 1104 50-51 0.2693896343165818 1104 52-53 0.24463878927143412 1104 54-55 0.30274481038355816 1104 56-57 0.3032835464318566 1104 58-59 0.27342934008311914 1104 60-61 0.372160921659642 1104 62-63 0.2790698215318983 1104 64-65 0.2293571234209537 1104 66-67 0.1840636542988463 1104 68-69 0.22839919489648253 1104 70-71 0.16576069495015844 1104 72-73 0.1622690868137937 1104 74-75 0.08831868812507793 1104 76-77 0.16256550562223282 1104 78-79 0.13198983746377024 1104 80-81 0.13217918170948906 1104 82-83 0.014814849614744219 1104 84-85 0.07918173422504537 1104 86-87 0.05800673351547658 1104 88-89 0.12611108885942457 1104 90-91 0.10116543128942013 1104 92-93 0.16063880190033686 1104 94-95 0.10826559503111 1104 96-97 0.05952364229797169 1104 98-99 0.0450143457745007 1104 100-101 0.10150843065136073 1104 102-103 0.03854634239183241 1104 104-105 0.11428946834222842 1104 106-107 0.11457544540191122 1104 108-109 0.09291493687128849 1104 110-111 0.12730551228959186 1104 112-113 0.0869986312637181 1104 114-115 0.10405350505399014 1104 116-117 0.09180590917408793 1104 118-119 0.1345571259074525 1104 120-121 0.12880687026802562 1104 122-123 0.10697669461156423 1104 124-125 0.2216188519587483 1105 1 0.14837537571275305 1105 2 0.5028252048646245 1105 3 0.10326302043826985 1105 4 0.3946224803934868 1105 5 0.13458329219245257 1105 6 0.08392405158223681 1105 7 0.06994301261926239 1105 8 0.03617544102727521 1105 9 0.11324979195622831 1105 10-11 0.12196020220801529 1105 12-13 0.08849520132113753 1105 14-15 0.14161751567249325 1105 16-17 0.21323728981657553 1105 18-19 0.1343578722434131 1105 20-21 0.09037875950804874 1105 22-23 0.09815027055896763 1105 24-25 0.061524941387553156 1105 26-27 0.08634191977587591 1105 28-29 0.1654655007593675 1105 30-31 0.11748390272795461 1105 32-33 0.12660138873448545 1105 34-35 0.15838747309301482 1105 36-37 0.19312215463147453 1105 38-39 0.14885099709526628 1105 40-41 0.1849018384741612 1105 42-43 0.12659668366053722 1105 44-45 0.1973918305099005 1105 46-47 0.16065838082550243 1105 48-49 0.1141057355051629 1105 50-51 0.06645774039940022 1105 52-53 0.0843694590529509 1105 54-55 0.15608018637911414 1105 56-57 0.12339162002106718 1105 58-59 0.18673911894463657 1105 60-61 0.1247189048544044 1105 62-63 0.1539592057805237 1105 64-65 0.04221357079321564 1105 66-67 0.004242098967331742 1105 68-69 -0.02492893913434102 1105 70-71 0.07427898398394461 1105 72-73 0.01631578652368404 1105 74-75 0.03933665201789438 1105 76-77 0.015491029600248396 1105 78-79 -0.008678185059629584 1105 80-81 -0.06072677124642212 1105 82-83 -0.0322869886317676 1105 84-85 0.019937895002641426 1105 86-87 -0.0017511855425098588 1105 88-89 -0.0176985787980648 1105 90-91 -0.0083452283788219 1105 92-93 -0.057157889020082564 1105 94-95 -0.07149707781197279 1105 96-97 -0.045408080314626176 1105 98-99 -0.12047163518256454 1105 100-101 -0.10630558838603221 1105 102-103 -0.24213521286106499 1105 104-105 -0.05514105798970448 1105 106-107 -0.036608374985846126 1105 108-109 -0.06747254589097906 1105 110-111 -0.09198364347172472 1105 112-113 -0.08885152584207034 1105 114-115 -0.05977969677419992 1105 116-117 -0.12502903443520097 1105 118-119 -0.10660805085046832 1105 120-121 -0.08153713025419407 1105 122-123 -0.004342324620445481 1105 124-125 -4.947807796256143E-4 1106 1 0.14728608805350518 1106 2 0.43081876314524337 1106 3 0.3681083331624819 1106 4 0.4290011704216141 1106 5 0.11986067425418412 1106 6 0.13600201825680358 1106 7 0.09616652437635054 1106 8 0.008038409266454494 1106 9 0.17767568408941514 1106 10-11 0.13610249163760813 1106 12-13 0.1466895111788986 1106 14-15 0.26521019945425905 1106 16-17 0.1512658606832744 1106 18-19 0.0731540013768992 1106 20-21 0.06118180363538528 1106 22-23 0.09387896845338872 1106 24-25 0.12595793804047872 1106 26-27 0.1088185402330879 1106 28-29 0.20999619833568062 1106 30-31 0.09099528162933268 1106 32-33 0.11921555161351449 1106 34-35 -0.0017941897471160928 1106 36-37 0.023032280025432783 1106 38-39 -0.012397559309540895 1106 40-41 -0.015761810113275487 1106 42-43 -0.11512638875089465 1106 44-45 -0.016533795379380933 1106 46-47 -0.015061812175396483 1106 48-49 -0.08952879092646526 1106 50-51 -0.08640126962277606 1106 52-53 -0.12306798443505329 1106 54-55 -0.1535137949094718 1106 56-57 -0.14108736421521684 1106 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-0.867466728477261 2216 78-79 -0.8098651639353633 2216 80-81 -0.8508550116392897 2216 82-83 -0.8525681614470386 2216 84-85 -0.923976955264397 2216 86-87 -0.9182323822181218 2216 88-89 -0.9750653967020035 2216 90-91 -0.9384274245077435 2216 92-93 -0.9497680255489236 2216 94-95 -1.009554179261137 2216 96-97 -1.0705705042842837 2216 98-99 -1.0556649222816112 2216 100-101 -1.1635058743396378 2216 102-103 -1.2035909634917275 2216 104-105 -1.1421069135443283 2216 106-107 -1.2279238613072678 2216 108-109 -1.324957395685491 2216 110-111 -1.241244008022786 2216 112-113 -1.217268718570974 2216 114-115 -1.2750027313759027 2216 116-117 -1.4077551160276833 2216 118-119 -1.5728846769009444 2216 120-121 -1.6847586809142996 2216 122-123 -1.791435182859935 2216 124-125 -2.0627641922622786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67.0 3 19.0 4 49.0 5 221.0 6 658.0 7 1380.0 8 2252.0 9 4397.0 10 7876.0 11 10593.0 12 11970.0 13 16286.0 14 24190.0 15 23966.0 16 11827.0 17 9247.0 18 9076.0 19 8654.0 20 8357.0 21 8331.0 22 8683.0 23 9798.0 24 11530.0 25 14337.0 26 18136.0 27 22949.0 28 29788.0 29 38570.0 30 49911.0 31 64754.0 32 85734.0 33 114269.0 34 161268.0 35 248850.0 36 452389.0 37 851681.0 38 420526.0 39 2989.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42058104304429 18.987445823017573 12.421570342173656 24.170402791764484 2 14.949797076630691 21.562307454215688 38.09871795242655 25.389177516727074 3 20.758912106174364 25.40707790526634 29.8429181172448 23.991091871314495 4 13.351675490620767 17.198393970446684 35.718030733539244 33.7318998053933 5 12.703565041376521 37.98211440791039 34.85882517144698 14.455495379266106 6 32.34601543606299 34.039071811249585 17.499508221381376 16.115404531306048 7 25.808131247789795 33.08867079503814 22.16234725616128 18.940850701010785 8 26.65478970399678 33.95800082297444 21.091106452249765 18.29610302077902 9 27.27227364406283 14.824134412408545 20.643605061943653 37.25998688158497 10-11 24.641281585435504 26.669413534149953 26.97868019827345 21.710624682141088 12-13 26.237269675321578 24.541267152761005 27.3792562785578 21.84220689335962 14-15 24.77390467023222 24.50153442170544 26.016556639813533 24.708004268248807 16-17 22.325410102368075 27.930821954834094 28.48658652977815 21.25718141301968 18-19 22.658590717745078 25.963035575203445 30.858232890195104 20.520140816856365 20-21 22.06100396138307 25.661358968995412 30.91867530598073 21.358961763640792 22-23 21.49017312113417 26.148457935375536 29.22389099132261 23.137477952167686 24-25 21.95031201434203 26.750285112189932 28.69317010765923 22.606232765808812 26-27 22.49607496154511 25.397186410941945 29.202539216033685 22.904199411479265 28-29 22.189600887338877 24.923103625017966 30.6452461470455 22.242049340597653 30-31 23.049395099324627 25.76358359807606 29.869705356348653 21.317315946250655 32-33 21.02280246660915 25.94488385429737 30.6664827388705 22.365830940222985 34-35 20.613651217512434 26.408553005656145 30.902677650508796 22.07511812632262 36-37 22.796843914725965 25.75076530114139 29.504519489235157 21.947871294897485 38-39 23.272910762234876 25.106776955847927 29.637674294487443 21.98263798742975 40-41 22.07700237599604 25.989187789745145 29.53681998367791 22.39698985058091 42-43 23.110159973792097 25.75588177227328 29.209951771383775 21.924006482550844 44-45 23.964538335205155 24.901250299214126 28.934548221022876 22.199663144557846 46-47 22.385537489812258 26.018557422715975 30.274412799060453 21.32149228841132 48-49 23.329119627072533 25.868046390302496 29.599074768457083 21.20375921416789 50-51 21.849392785161005 26.341003580445026 29.38964296071201 22.419960673681956 52-53 21.61067234408142 26.968431192322186 27.660944656053815 23.75995180754258 54-55 22.187278753302202 27.079691840186754 27.18044835473814 23.552581051772904 56-57 22.677791044042152 26.03452153582361 27.620953015969903 23.666734404164337 58-59 22.71134641655379 25.37697363806047 28.796602373897972 23.11507757148777 60-61 23.342407988492823 26.232979145769892 28.314153497026663 22.11045936871063 62-63 21.391568055574638 26.403160568966054 29.05521015859976 23.150061216859548 64-65 20.702345043242318 26.59726104271874 29.577849548991203 23.12254436504774 66-67 22.695707732705422 26.387467646907808 28.01819366512172 22.898630955265048 68-69 23.204570617787674 25.5242846160911 28.17989584817351 23.091248917947713 70-71 21.874016932445947 26.42257784810264 28.146702063727723 23.556703155723685 72-73 23.054059585374194 26.061188655680656 27.925518643842263 22.959233115102883 74-75 23.570985884325086 25.2374006446392 27.878873783346553 23.312739687689156 76-77 22.177497796120736 26.09145357679299 29.526775234688735 22.20427339239754 78-79 22.799375031186973 25.943256708001005 29.14314837621647 22.114219884595553 80-81 21.34956960172521 26.3747397469896 28.943117858183715 23.332572793101477 82-83 21.016203484407235 26.842761260033164 27.427991544624668 24.71304371093493 84-85 21.631644451901195 26.851656326453277 26.908082144130454 24.60861707751508 86-87 22.072908448071253 25.540194490988867 27.583799842202968 24.803097218736916 88-89 22.094007111714077 25.104209680580336 28.499250427939476 24.30253277976611 90-91 22.535668854756583 26.10754424572368 27.897133980672393 23.459652918847343 92-93 20.876286982323407 25.975727316315073 28.62459493096922 24.5233907703923 94-95 20.22273238594267 26.199512503972972 29.129333975999806 24.44842113408455 96-97 22.12344996888457 25.838986602466306 27.830880318957558 24.20668310969156 98-99 22.45813986999408 25.096388903302653 27.810845060160233 24.63462616654303 100-101 21.186045229251395 25.89701427640339 27.877848297507484 25.039092196837732 102-103 22.120782910994645 25.478724442898397 27.809916130110064 24.590576515996894 104-105 22.64451804653713 24.53275168552248 28.036815673532683 24.785914594407704 106-107 21.19323671235522 25.49507085460038 29.42256515912698 23.889127273917417 108-109 21.750094075427064 25.472316508614522 28.685835450369495 24.091753965588918 110-111 20.467654590031938 25.73197830650337 28.82122032337725 24.97914678008745 112-113 20.17562924924056 26.036308404455372 27.475860697237092 26.312201649066974 114-115 20.442740691304166 26.3836121375229 27.06326856539283 26.1103786057801 116-117 20.844426341940544 25.268547269238894 27.440130309089806 26.44689607973076 118-119 20.70515148183404 24.92623741750309 28.251268014779278 26.117343085883586 120-121 21.102100205425987 25.691665013699588 27.768074946401374 25.43815983447305 122-123 19.64461928040114 25.786517727452374 28.231000902906622 26.33786208923986 124-125 19.083982576913037 26.127579346569153 28.631117552320966 26.15732052419684 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1413.0 1 1038.5 2 1088.0 3 1465.5 4 956.5 5 677.0 6 1486.5 7 3138.0 8 4751.5 9 5210.5 10 4152.5 11 2878.0 12 2556.5 13 2756.5 14 3147.5 15 3588.5 16 3993.0 17 4439.0 18 4908.5 19 5267.5 20 5730.0 21 6450.5 22 7375.0 23 8655.5 24 10298.5 25 12291.5 26 15316.0 27 19104.0 28 21613.0 29 24172.0 30 28259.5 31 32259.0 32 36352.0 33 40970.0 34 46636.5 35 51241.0 36 55749.0 37 60800.5 38 63779.0 39 65217.0 40 66371.5 41 70443.5 42 74513.0 43 77699.0 44 82071.0 45 85922.0 46 89336.0 47 95283.0 48 104428.5 49 130560.5 50 220594.5 51 218427.5 52 118451.5 53 84533.0 54 77120.0 55 70969.0 56 63799.5 57 57143.0 58 53099.5 59 48493.5 60 41942.5 61 34833.5 62 28575.0 63 23214.5 64 18259.0 65 14082.0 66 10269.0 67 8176.0 68 6321.0 69 4387.5 70 3370.0 71 2744.0 72 2371.0 73 2213.5 74 1753.0 75 1369.5 76 902.5 77 458.5 78 263.0 79 156.5 80 93.5 81 44.0 82 19.0 83 9.0 84 5.5 85 3.0 86 1.5 87 0.5 88 0.5 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14561151412109874 2 7.231761317164079E-5 3 5.062232922014855E-4 4 0.0 5 0.0 6 0.003832833498096962 7 0.0 8 0.0 9 0.0 10-11 1.2655582305037138E-4 12-13 0.004845280082499933 14-15 0.0014825110700186362 16-17 6.689379218376774E-4 18-19 0.0 20-21 1.2655582305037138E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.988734362220122E-4 30-31 0.0 32-33 0.0 34-35 1.8079403292910197E-5 36-37 0.0 38-39 0.0 40-41 3.6158806585820394E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 6.146997119589467E-4 96-97 0.0024949576544216075 98-99 0.003200054382845105 100-101 1.8079403292910197E-5 102-103 0.0037605158849253213 104-105 0.020248931688059423 106-107 0.016036430720811345 108-109 0.018422911955475494 110-111 0.015096301749580016 112-113 0.01366802888944011 114-115 0.039521575598301696 116-117 0.0388345582731711 118-119 0.02263541292272357 120-121 0.012601344095158408 122-123 0.0023503224280783256 124-125 0.0018802579424626606 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2765578.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.785300138010726 #Duplication Level Percentage of deduplicated Percentage of total 1 83.9196909629115 46.81485147855125 2 10.486794502370868 11.700179576007995 3 2.6965011895050828 4.51275384537132 4 1.0333372637818847 2.305801176154528 5 0.5062335876517867 1.4120196313548443 6 0.29307483481372865 0.9809560573789063 7 0.18816908934763577 0.7347948388167814 8 0.12268630628615552 0.5475273935197679 9 0.09338714076448451 0.46886667089196826 >10 0.5602135008239867 5.929511167991013 >50 0.05506455222523206 2.091861483828159 >100 0.038474728604407135 3.983166141862535 >500 0.0027653554906878824 1.09227166939194 >1k 0.0016832598638969719 2.3308949987539886 >5k 9.618627793696982E-4 3.4159899368824997 >10k+ 9.618627793696982E-4 11.678553933242569 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 46710 1.6889778556236708 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 40469 1.4633107437215656 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 26331 0.9520975362112369 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 13611 0.49215751643960143 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 13353 0.48282854434045974 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11637 0.42078003223919197 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 11027 0.3987231602218415 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10981 0.3970598551188938 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 8604 0.31111037186439866 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7013 0.2535817105863585 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 6860 0.24804941317872792 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 6720 0.24298718025671304 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 5825 0.2106250483624038 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 5504 0.19901807144835545 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 5339 0.1930518683616951 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 5065 0.1831443553571803 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 4432 0.160255830788356 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4324 0.1563506796770874 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 4197 0.1517585112406882 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 3909 0.14134477494397193 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.6158806585820394E-5 0.0 0.0 0.0 0.0 7 7.231761317164079E-5 0.0 0.0 0.0 0.0 8 7.231761317164079E-5 0.0 0.0 0.0 0.0 9 7.231761317164079E-5 0.0 0.0 3.6158806585820394E-5 0.0 10-11 7.231761317164079E-5 0.0 0.0 1.8079403292910198E-4 0.0 12-13 7.231761317164079E-5 0.0 0.0 3.6158806585820396E-4 0.0 14-15 7.231761317164079E-5 0.0 0.0 6.327791152518569E-4 0.0 16-17 1.4463522634328157E-4 0.0 0.0 0.0014463522634328158 0.0 18-19 1.988734362220122E-4 0.0 0.0 0.002115290185270493 0.0 20-21 2.169528395149224E-4 0.0 0.0 0.003254292592723836 0.0 22-23 2.169528395149224E-4 0.0 0.0 0.004845280082499933 0.0 24-25 3.254292592723836E-4 0.0 0.0 0.006526664588740582 0.0 26-27 3.6158806585820396E-4 0.0 0.0 0.008985463436576368 0.0 28-29 3.977468724440244E-4 0.0 0.0 0.013360679033460637 0.0 30-31 4.339056790298448E-4 0.0 0.0 0.025003814754094807 0.0 32-33 4.339056790298448E-4 0.0 0.0 0.04751267185376801 0.0 34-35 4.339056790298448E-4 0.0 0.0 0.084756242637163 0.0 36-37 4.7006448561566516E-4 0.0 0.0 0.14214026868885998 0.0 38-39 5.062232922014855E-4 0.0 0.0 0.2212376580953421 0.0 40-41 5.062232922014855E-4 0.0 0.0 0.31224937427185206 0.0 42-43 5.423820987873059E-4 0.0 0.0 0.3930281481845748 0.0 44-45 6.146997119589468E-4 0.0 0.0 0.49190440479350067 0.0 46-47 7.593349383022283E-4 0.0 0.0 0.6209371060950007 0.0 48-49 8.316525514738691E-4 0.0 0.0 0.747131341079514 0.0 50-51 8.316525514738691E-4 0.0 0.0 0.8696735365988593 0.0 52-53 8.316525514738691E-4 0.0 0.0 1.0001887489703778 0.0 54-55 8.316525514738691E-4 0.0 0.0 1.1450228487498815 0.0 56-57 8.678113580596896E-4 0.0 0.0 1.3226168272961383 0.0 58-59 8.678113580596896E-4 0.0 0.0 1.522448471892675 0.0 60-61 8.678113580596896E-4 0.0 0.0 1.7156630548840062 0.0 62-63 8.678113580596896E-4 0.0 0.0 1.902332893883304 0.0 64-65 8.678113580596896E-4 0.0 0.0 2.113898071216939 0.0 66-67 9.039701646455099E-4 0.0 0.0 2.3744765108776535 0.0 68-69 9.039701646455099E-4 0.0 0.0 2.6753539404782654 0.0 70-71 9.220495679384201E-4 0.0 0.0 3.0309758032498086 0.0 72-73 9.582083745242405E-4 0.0 0.0 3.353765469641427 0.0 74-75 0.001012446584402971 0.0 0.0 3.6698657568146693 0.0 76-77 0.001012446584402971 0.0 0.0 4.043024640780336 0.0 78-79 0.001012446584402971 0.0 0.0 4.441856277421936 0.0 80-81 0.0012293994239178936 0.0 0.0 4.864498488200296 0.0 82-83 0.0012293994239178936 0.0 0.0 5.321220374185794 0.0 84-85 0.0012474788272108038 0.0 0.0 5.778159213010806 0.0 86-87 0.001265558230503714 0.0 0.0 6.261313186610538 0.0 88-89 0.001265558230503714 0.0 0.0 6.801579995212574 0.0 90-91 0.0013017170370895343 0.0 0.0 7.361209844741316 0.0 92-93 0.0013378758436753547 0.0 0.0 7.9233708107310665 0.0 94-95 0.0013378758436753547 0.0 0.0 8.512235055384444 0.0 96-97 0.0013378758436753547 0.0 0.0 9.119124465120855 0.0 98-99 0.0014101934568469955 0.0 0.0 9.78849629263756 0.0 100-101 0.0014463522634328158 0.0 0.0 10.593445565447801 0.0 102-103 0.0014825110700186362 0.0 0.0 11.347880985457651 0.0 104-105 0.001554828683190277 0.0 0.0 12.070858243737836 0.0 106-107 0.001554828683190277 0.0 0.0 12.826414586751847 0.0 108-109 0.001554828683190277 0.0 0.0 13.576131282502248 0.0 110-111 0.001554828683190277 0.0 0.0 14.367756035085613 0.0 112-113 0.001554828683190277 0.0 0.0 15.231680321437327 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGCGA 135 8.247354E-5 26.445835 8 GTTATCA 1565 0.0 24.743736 1 GAGTACT 16185 0.0 22.076204 12-13 AGTACTC 1770 0.0 21.512531 5 GCGCGAC 145 0.003866743 20.519062 9 AGTACTT 16575 0.0 20.461872 12-13 GTACTTT 17545 0.0 20.397404 14-15 CATGGGG 7415 0.0 20.299913 4 TTATCAA 2100 0.0 18.415304 2 ACTTTTT 20055 0.0 17.932905 16-17 GTGTAGC 1340 0.0 17.783674 1 ATGGGCG 450 1.8590072E-9 17.187616 5 TACTTTT 19920 0.0 16.90518 14-15 AAAGTAC 2050 0.0 16.252455 3 GTATAGG 990 0.0 16.24781 1 AAGTACT 2345 0.0 16.237604 4 TAAAGCG 260 3.667139E-4 16.018047 5 CGTACGC 115 0.0029934149 15.52031 78-79 TAACGCA 235 0.0034533932 15.190365 4 GTCTAGA 830 0.0 15.073271 1 >>END_MODULE