##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364164_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2704394 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.55767724673254 31.0 31.0 34.0 26.0 34.0 2 30.597603011987157 31.0 31.0 34.0 26.0 34.0 3 30.76364871390781 31.0 31.0 34.0 26.0 34.0 4 34.050034129642356 37.0 35.0 37.0 28.0 37.0 5 34.15902046817143 37.0 35.0 37.0 30.0 37.0 6 33.970736512505205 37.0 35.0 37.0 29.0 37.0 7 33.89440813727585 37.0 35.0 37.0 29.0 37.0 8 33.63709651774113 37.0 35.0 37.0 27.0 37.0 9 34.9213076940712 39.0 35.0 39.0 26.0 39.0 10-11 34.938753931564705 39.0 35.0 39.0 27.0 39.0 12-13 34.84541176322681 39.0 35.0 39.0 26.0 39.0 14-15 36.04246884884377 40.0 36.0 41.0 26.0 41.0 16-17 36.14670384566746 40.0 36.0 41.0 27.0 41.0 18-19 36.16443776313658 40.0 36.0 41.0 27.0 41.0 20-21 36.2277421485183 40.0 36.0 41.0 27.0 41.0 22-23 36.27832224150771 40.0 36.0 41.0 27.0 41.0 24-25 36.35256105434341 40.0 36.0 41.0 27.5 41.0 26-27 36.25674125145966 40.0 36.0 41.0 27.0 41.0 28-29 36.21312168271339 40.0 36.0 41.0 28.0 41.0 30-31 36.27630404445506 40.0 36.0 41.0 29.0 41.0 32-33 36.007453610679505 40.0 35.0 41.0 28.0 41.0 34-35 36.10039421031107 40.0 35.0 41.0 29.0 41.0 36-37 36.09092628514928 40.0 35.5 41.0 29.0 41.0 38-39 35.983716130120094 40.0 35.0 41.0 28.5 41.0 40-41 35.76426530305865 40.0 35.0 41.0 28.0 41.0 42-43 35.58460435128905 40.0 35.0 41.0 27.0 41.0 44-45 35.383548403080326 40.0 35.0 41.0 27.0 41.0 46-47 35.17397927225101 39.0 35.0 41.0 26.0 41.0 48-49 34.96972371629282 39.0 34.5 41.0 25.0 41.0 50-51 34.85215689725683 39.0 34.0 41.0 25.0 41.0 52-53 34.70791534073807 39.0 34.0 41.0 24.5 41.0 54-55 34.47805959486672 39.0 34.0 41.0 23.5 41.0 56-57 34.173129913762565 38.0 33.0 40.5 22.5 41.0 58-59 33.890390416485175 38.0 33.0 40.0 21.5 41.0 60-61 33.5558443037516 38.0 33.0 40.0 19.5 41.0 62-63 33.062587219169984 37.0 32.0 40.0 14.0 41.0 64-65 32.8391033259207 37.0 32.0 40.0 13.0 41.0 66-67 32.43253405383979 36.0 31.5 39.0 8.0 41.0 68-69 32.050448640249904 36.0 31.0 39.0 4.5 41.0 70-71 31.62277482497003 35.0 31.0 39.0 2.0 40.5 72-73 31.344603079285044 35.0 31.0 38.0 2.0 40.0 74-75 30.984103832503695 35.0 31.0 37.0 2.0 39.0 76-77 30.433354755261252 35.0 30.0 37.0 2.0 39.0 78-79 29.990442590835507 35.0 30.0 36.0 2.0 38.5 80-81 29.739099776142087 35.0 30.0 36.0 2.0 37.0 82-83 29.48186321963442 35.0 30.0 35.0 2.0 37.0 84-85 29.197569030252247 35.0 30.0 35.0 2.0 36.5 86-87 28.882495117205558 34.0 29.0 35.0 2.0 36.0 88-89 28.619621068527735 34.0 29.0 35.0 2.0 36.0 90-91 28.40255894666236 34.0 29.0 35.0 2.0 35.5 92-93 28.131458840686676 34.0 28.5 35.0 2.0 35.0 94-95 28.07248037822891 34.0 29.0 35.0 2.0 35.0 96-97 27.9996842176103 34.0 28.5 35.0 2.0 35.0 98-99 27.846984573993286 34.0 28.0 35.0 2.0 35.0 100-101 27.556649474891604 34.0 27.0 35.0 2.0 35.0 102-103 26.430108186898803 32.5 24.5 34.5 2.0 35.0 104-105 27.20641666857714 34.0 26.0 35.0 2.0 35.0 106-107 27.231592733898978 34.0 26.0 35.0 2.0 35.0 108-109 27.166211173371927 34.0 25.0 35.0 2.0 35.0 110-111 27.16026677325863 34.0 25.0 35.0 2.0 35.0 112-113 27.03713734019525 34.0 25.0 35.0 2.0 35.0 114-115 26.858917746452626 34.0 25.0 35.0 2.0 35.0 116-117 26.564211242888426 34.0 25.0 35.0 2.0 35.0 118-119 26.297759498061303 34.0 24.5 35.0 2.0 35.0 120-121 25.984689915744525 34.0 23.5 35.0 2.0 35.0 122-123 25.593936016719457 34.0 19.0 35.0 2.0 35.0 124-125 24.561454248160587 32.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5111833852962988 1101 2 0.5704615610187709 1101 3 0.5536836799812335 1101 4 0.7282784706842804 1101 5 0.5139500196415696 1101 6 0.5795133264494368 1101 7 0.6144766856372854 1101 8 0.6407046732241284 1101 9 0.8464412132910226 1101 10-11 0.7767864228911279 1101 12-13 0.6973556240001315 1101 14-15 0.8233643281723886 1101 16-17 0.7138871944842293 1101 18-19 0.7585513014553698 1101 20-21 0.7386794992231955 1101 22-23 0.7333217782856138 1101 24-25 0.7379667092784743 1101 26-27 0.8222169564125323 1101 28-29 0.866970622922203 1101 30-31 0.7695902586019159 1101 32-33 0.8640369460056192 1101 34-35 0.8272926374286769 1101 36-37 0.8268756837957412 1101 38-39 0.8268099536325266 1101 40-41 0.8810003087204592 1101 42-43 0.9494274769678412 1101 44-45 0.9393216678366372 1101 46-47 0.9257191371582962 1101 48-49 0.9546774233632647 1101 50-51 0.8799678878828487 1101 52-53 0.8687335788700281 1101 54-55 0.9460836094145648 1101 56-57 0.9270141479915281 1101 58-59 0.9768168080901347 1101 60-61 0.971625235838971 1101 62-63 1.0223157068513586 1101 64-65 0.9320246739766773 1101 66-67 0.9231695141541394 1101 68-69 0.9379599986337794 1101 70-71 0.9063266038434499 1101 72-73 0.8591009274789165 1101 74-75 0.8831412845087243 1101 76-77 0.8493526380413634 1101 78-79 0.8309452684538705 1101 80-81 0.8293696414782765 1101 82-83 0.81073026163374 1101 84-85 0.7884331863372189 1101 86-87 0.7682986347419956 1101 88-89 0.6399669279973601 1101 90-91 0.6040843648022864 1101 92-93 0.6993184620743129 1101 94-95 0.6335694923975836 1101 96-97 0.5544064087018796 1101 98-99 0.6531469459447621 1101 100-101 0.5952901150477352 1101 102-103 0.5255090123735968 1101 104-105 0.6038425400683458 1101 106-107 0.5988534018070091 1101 108-109 0.5986460189525147 1101 110-111 0.6594417709954961 1101 112-113 0.5914029820171791 1101 114-115 0.645540338505679 1101 116-117 0.6072463008153051 1101 118-119 0.6262213001636994 1101 120-121 0.5865038455607134 1101 122-123 0.5282093851926852 1101 124-125 0.4866750612810762 1102 1 0.4812811812561648 1102 2 0.5627328963935554 1102 3 0.5704530243367856 1102 4 0.6547880747800932 1102 5 0.5256891658634757 1102 6 0.6985708299896984 1102 7 0.7036229075421332 1102 8 0.6376775977832594 1102 9 0.7278406153800816 1102 10-11 0.7611517779461394 1102 12-13 0.8544328198704321 1102 14-15 0.9197895711493516 1102 16-17 0.8100600803122902 1102 18-19 0.8414648464378871 1102 20-21 0.7884455998513715 1102 22-23 0.8399702090719714 1102 24-25 0.8830666233439075 1102 26-27 0.9745323483831001 1102 28-29 0.9617700685837178 1102 30-31 0.8994286168885495 1102 32-33 1.008622160418419 1102 34-35 0.9211407511043816 1102 36-37 0.9511116528592893 1102 38-39 0.9461028694771656 1102 40-41 0.909594128693584 1102 42-43 0.9869730162375205 1102 44-45 0.9754765195087316 1102 46-47 0.9083582361766958 1102 48-49 0.928363895325603 1102 50-51 0.9141755953664159 1102 52-53 0.8950465017346758 1102 54-55 0.9040488966901918 1102 56-57 0.8839385376745454 1102 58-59 0.875923572714143 1102 60-61 0.8839268903820141 1102 62-63 0.9836487819775428 1102 64-65 1.003048687242071 1102 66-67 1.0162048804653665 1102 68-69 0.9618676613345443 1102 70-71 0.9494094762366636 1102 72-73 1.0085341829466579 1102 74-75 0.9911298709455849 1102 76-77 0.8943106390338436 1102 78-79 0.8295594222404397 1102 80-81 0.8997682084285685 1102 82-83 0.888836312153586 1102 84-85 0.8178706174987909 1102 86-87 0.8672196112491797 1102 88-89 0.9082401273050422 1102 90-91 0.9031442285173235 1102 92-93 0.9076825583694443 1102 94-95 0.942251304527673 1102 96-97 1.0081584814194713 1102 98-99 0.9877849314448142 1102 100-101 0.9091098461088443 1102 102-103 0.75142766501639 1102 104-105 0.8754299694143981 1102 106-107 0.9042285485614663 1102 108-109 0.8194022431570147 1102 110-111 0.8315466232727609 1102 112-113 0.8783130896169418 1102 114-115 0.7482539343227224 1102 116-117 0.7582881061674556 1102 118-119 0.7352828583631918 1102 120-121 0.6852087753001257 1102 122-123 0.7076095982135904 1102 124-125 0.6944491138827154 1103 1 0.29823273998840705 1103 2 0.3001978859234704 1103 3 0.30560317567818274 1103 4 0.4224314283804347 1103 5 0.2902675857102395 1103 6 0.3828208666530273 1103 7 0.43004177821555345 1103 8 0.3884869557062629 1103 9 0.5221492652801842 1103 10-11 0.4944132271211501 1103 12-13 0.4342630025619769 1103 14-15 0.2603428147116347 1103 16-17 0.31083854487629026 1103 18-19 0.31051294631599546 1103 20-21 0.37136836404968676 1103 22-23 0.32995931826136626 1103 24-25 0.44167685493591335 1103 26-27 0.5635245757582155 1103 28-29 0.5503332148499496 1103 30-31 0.46788150872333034 1103 32-33 0.5666126616120764 1103 34-35 0.42300631330341076 1103 36-37 0.48172817371411014 1103 38-39 0.479342440864869 1103 40-41 0.5237395606837225 1103 42-43 0.5376857272319455 1103 44-45 0.45876814079701944 1103 46-47 0.4677051601838471 1103 48-49 0.48621285204364995 1103 50-51 0.54522576521979 1103 52-53 0.49491090753637934 1103 54-55 0.4600539886850967 1103 56-57 0.4835743085780493 1103 58-59 0.5419837919247641 1103 60-61 0.5757381668186667 1103 62-63 0.5613751509620499 1103 64-65 0.5070366352258233 1103 66-67 0.4983217727617415 1103 68-69 0.5351336663784494 1103 70-71 0.48312843562331054 1103 72-73 0.4895896419354244 1103 74-75 0.4047093053446815 1103 76-77 0.3119972072745085 1103 78-79 0.4562391434549191 1103 80-81 0.4927686387718566 1103 82-83 0.42485676668264816 1103 84-85 0.35330758422772135 1103 86-87 0.2719263407123087 1103 88-89 0.27524517842934415 1103 90-91 0.19593098981142987 1103 92-93 0.1342176698139319 1103 94-95 0.24389285982197606 1103 96-97 0.13867835534571782 1103 98-99 0.21977344200024618 1103 100-101 0.12991610267518539 1103 102-103 0.08057410675055365 1103 104-105 0.12619346812937593 1103 106-107 0.04824220895570264 1103 108-109 0.11820269129103167 1103 110-111 0.09912260050063182 1103 112-113 0.13791491388782262 1103 114-115 0.11085313566328026 1103 116-117 0.08981261529962836 1103 118-119 0.1995224730256382 1103 120-121 0.18809544114937893 1103 122-123 0.1426745824543687 1103 124-125 0.24785724039883306 1104 1 0.2543697918561989 1104 2 0.36588976030239095 1104 3 0.23641420897682508 1104 4 0.3063430632067039 1104 5 0.24110966559639735 1104 6 0.3455403830789052 1104 7 0.33894191757239867 1104 8 0.17331176004807958 1104 9 0.35997071212172926 1104 10-11 0.2613197404364911 1104 12-13 0.2805201247550002 1104 14-15 0.35857110121048663 1104 16-17 0.34528590973837225 1104 18-19 0.29974842409828284 1104 20-21 0.295931800812852 1104 22-23 0.2734564693841577 1104 24-25 0.30716063485794365 1104 26-27 0.367830466965799 1104 28-29 0.4705643590460582 1104 30-31 0.3959396857947226 1104 32-33 0.35195796669772506 1104 34-35 0.2792958344921175 1104 36-37 0.34333730423325903 1104 38-39 0.4435246749255626 1104 40-41 0.4269696405934056 1104 42-43 0.4295529913102243 1104 44-45 0.41623502149698055 1104 46-47 0.46810035737083666 1104 48-49 0.5158011319568558 1104 50-51 0.45910852907168476 1104 52-53 0.4720724379920185 1104 54-55 0.5344661321344333 1104 56-57 0.5365553101833029 1104 58-59 0.5520004912256269 1104 60-61 0.5798040937768718 1104 62-63 0.563617326104783 1104 64-65 0.5192873364200636 1104 66-67 0.423581876132765 1104 68-69 0.44807544814681677 1104 70-71 0.44142740014906323 1104 72-73 0.3663076413121402 1104 74-75 0.3836306128293785 1104 76-77 0.34507893388813926 1104 78-79 0.4028759395411683 1104 80-81 0.40008158014202166 1104 82-83 0.3647339915410832 1104 84-85 0.30020962871606827 1104 86-87 0.31686410053637815 1104 88-89 0.4086383705242227 1104 90-91 0.26590017842532987 1104 92-93 0.2667483782994111 1104 94-95 0.17272967119631844 1104 96-97 0.1992576564446935 1104 98-99 0.20410073653710725 1104 100-101 0.26399737652608124 1104 102-103 0.17723483458134837 1104 104-105 0.16006911713829908 1104 106-107 0.18172454238899505 1104 108-109 0.13884790758813637 1104 110-111 0.17005246931703866 1104 112-113 0.1201161639833721 1104 114-115 0.14478429220746492 1104 116-117 0.06704441217967982 1104 118-119 0.06108409285781846 1104 120-121 -0.0968542551467877 1104 122-123 -0.034421679607053335 1104 124-125 -0.0475421022131961 1105 1 0.23805350330326291 1105 2 0.28894227288166263 1105 3 0.3034721547756192 1105 4 0.30217901870442176 1105 5 0.2260178447559582 1105 6 0.23739260406400575 1105 7 0.27733843438889494 1105 8 0.19668855723921297 1105 9 0.31052693369912276 1105 10-11 0.36610908927595176 1105 12-13 0.3506862821759853 1105 14-15 0.4378449633809822 1105 16-17 0.40243910773479996 1105 18-19 0.3632601690217214 1105 20-21 0.39212763591406485 1105 22-23 0.3827234973162632 1105 24-25 0.41788848124704003 1105 26-27 0.464512415748878 1105 28-29 0.6006717420168499 1105 30-31 0.5258383966865026 1105 32-33 0.3426218844818365 1105 34-35 0.41727708589812096 1105 36-37 0.4651979825883714 1105 38-39 0.5228509044477647 1105 40-41 0.5051875844558325 1105 42-43 0.511480958437545 1105 44-45 0.4971335245140125 1105 46-47 0.4774120356604499 1105 48-49 0.5334909600472884 1105 50-51 0.6022980681307786 1105 52-53 0.5045595027720537 1105 54-55 0.5244790317594052 1105 56-57 0.5579832673440137 1105 58-59 0.5714013563262981 1105 60-61 0.5673213003958324 1105 62-63 0.5019309777807379 1105 64-65 0.5347295543582291 1105 66-67 0.5494045048656133 1105 68-69 0.5347460350085598 1105 70-71 0.560036120067732 1105 72-73 0.47128382114373224 1105 74-75 0.4389715986113174 1105 76-77 0.42162285953162737 1105 78-79 0.33903726173849336 1105 80-81 0.4228173542476661 1105 82-83 0.38860071819717845 1105 84-85 0.3771339366782449 1105 86-87 0.3224185708401883 1105 88-89 0.30145453235898856 1105 90-91 0.28680782223621293 1105 92-93 0.20448803937813764 1105 94-95 0.24651079901650874 1105 96-97 0.30523174898597816 1105 98-99 0.19910616121383384 1105 100-101 0.1836048064822542 1105 102-103 0.06968328591445072 1105 104-105 0.07616967156588927 1105 106-107 0.10512807853784523 1105 108-109 0.12947765418027402 1105 110-111 0.24010691541625206 1105 112-113 0.2966107122544521 1105 114-115 0.36635648027431245 1105 116-117 0.25394952732235865 1105 118-119 0.29367159246627494 1105 120-121 0.2075399038574659 1105 122-123 0.20903559074117695 1105 124-125 0.29889233323600095 1106 1 0.11428239708592258 1106 2 0.08060530847422243 1106 3 0.12180727603066899 1106 4 0.030269126672621383 1106 5 0.02259593512410163 1106 6 0.09546932247496187 1106 7 0.032118703511052615 1106 8 -0.022886707053231703 1106 9 0.13698616675602437 1106 10-11 0.14409305850598741 1106 12-13 0.21097817945351238 1106 14-15 0.09557315345189465 1106 16-17 0.16792729800290118 1106 18-19 0.14854890993502323 1106 20-21 0.13115133480047803 1106 22-23 0.19130105746998538 1106 24-25 0.20509568909575648 1106 26-27 0.3005919183332608 1106 28-29 0.37405037727889834 1106 30-31 0.2126907223860428 1106 32-33 0.045695977838455804 1106 34-35 0.286140408617122 1106 36-37 0.2519042254523214 1106 38-39 0.1577911033274617 1106 40-41 0.2332071980072996 1106 42-43 0.2736673863178396 1106 44-45 0.2680996294071889 1106 46-47 0.29718815700505985 1106 48-49 0.2380277440832259 1106 50-51 0.3477220794493263 1106 52-53 0.31633579501222187 1106 54-55 0.3614817263769936 1106 56-57 0.3126412530276781 1106 58-59 0.39601730006003777 1106 60-61 0.3666088795479965 1106 62-63 0.4073560352868171 1106 64-65 0.49662291279275905 1106 66-67 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-1.6116070297838512 2216 82-83 -1.6098564308084384 2216 84-85 -1.711632494459952 2216 86-87 -1.7823629951792235 2216 88-89 -1.8355681074841534 2216 90-91 -1.8495013026103209 2216 92-93 -1.9772658588454242 2216 94-95 -2.079902194356883 2216 96-97 -1.969083791416331 2216 98-99 -1.946360448076458 2216 100-101 -2.022508504267389 2216 102-103 -1.9663013082056793 2216 104-105 -2.0406716156411697 2216 106-107 -2.164276876309888 2216 108-109 -2.0207390217527745 2216 110-111 -2.0996658687990326 2216 112-113 -2.1099199125260526 2216 114-115 -2.142635108857995 2216 116-117 -2.204546178550771 2216 118-119 -2.131451079051942 2216 120-121 -2.0156042596250003 2216 122-123 -2.0584257780704895 2216 124-125 -2.1823913654570717 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61219.0 3 11198.0 4 8465.0 5 8884.0 6 12205.0 7 11679.0 8 12320.0 9 13798.0 10 16789.0 11 19310.0 12 19787.0 13 20025.0 14 20447.0 15 20141.0 16 19197.0 17 18682.0 18 17971.0 19 17372.0 20 17458.0 21 18643.0 22 20103.0 23 24617.0 24 30124.0 25 31356.0 26 36751.0 27 36788.0 28 42227.0 29 53745.0 30 63451.0 31 75216.0 32 97858.0 33 128463.0 34 168194.0 35 232156.0 36 369312.0 37 641737.0 38 285536.0 39 1170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.536686813109235 18.24798777093844 10.73424159530694 23.481083820645384 2 13.289703445559859 23.95124796587878 36.86983110292096 25.889217485640405 3 21.345828234851847 30.749025437800753 27.822515637200603 20.0826306901468 4 13.454899774180332 19.08601541398477 32.85269417835482 34.606390633480075 5 11.410089575902882 42.181434945560895 33.193605871228094 13.214869607308128 6 30.400123639657274 31.697597014221163 20.901566411919934 17.000712934201633 7 25.620236256068264 34.53152749232099 21.640523922217735 18.207712329393008 8 29.142196404645677 32.942614185679965 21.95877031777962 15.956419091894736 9 30.926121085731527 16.512873032553813 18.802646697276753 33.75835918443791 10-11 23.542452291166725 30.576519477913326 24.695546989751694 21.185481241168258 12-13 26.41681009598724 28.031920556848274 24.515831977080047 21.035437370084438 14-15 26.41106291156002 27.267713335027498 23.26706390428253 23.054159849129956 16-17 22.382533261181294 30.800228196624346 27.814017893483594 19.003220648710766 18-19 23.81630869559968 29.685046755878496 28.282695449230705 18.215949099291116 20-21 22.24272382098779 29.751788858919774 27.40892237503994 20.596564945052496 22-23 20.702878462263623 31.386625886484964 23.392482804453742 24.518012846797674 24-25 21.45120454431883 32.329544852912086 22.569357688443013 23.649892914326074 26-27 23.09591758666515 28.953798095206956 23.757019704685177 24.193264613442718 28-29 22.674747281087633 28.47948421989541 26.65335750681017 22.192410992206785 30-31 24.52730241497352 30.415519032762006 24.86681748793405 20.19036106433042 32-33 19.408634445440114 31.088737719443788 27.169290104561583 22.33333773055451 34-35 18.093595906272927 32.09874111910144 27.53868617044302 22.268976804182607 36-37 23.243994708803108 30.178913098423653 25.06803882711926 21.509053365653983 38-39 24.917855122053364 28.50702009198741 24.61281452670442 21.962310259254807 40-41 21.157302004664775 31.549315644441396 24.981896853365903 22.31148549752793 42-43 23.939210209453147 30.04364086766391 24.617641172860385 21.399507750022558 44-45 24.877458149137542 28.61090141961914 24.738179667384415 21.773460763858903 46-47 21.418176496501435 30.305217279550234 28.616292864767928 19.6603133591804 48-49 22.87833966213891 29.87288470034853 28.079003996368186 19.169771641144372 50-51 20.120359172359393 30.623998562033027 27.51809017783514 21.737552087772436 52-53 19.137339686934617 31.864087793777312 24.16369460528153 24.834877914006544 54-55 20.263691676037322 32.111211956013136 23.18729844429821 24.43779792365133 56-57 22.04853382304291 29.22405141356486 24.09372230392712 24.63369245946511 58-59 21.789440148762935 28.378968967125413 26.593472164874953 23.2381187192367 60-61 23.002833744847102 30.46738036980909 25.091304811201887 21.438481074141922 62-63 18.93780458844087 30.828699655814017 26.795167470609023 23.43832828513609 64-65 17.653029730726505 31.440608185727065 27.366686388356847 23.53967569518958 66-67 22.00128232575939 30.261940939113295 24.97087100296777 22.765905732159545 68-69 23.582092323689977 28.4385932335574 24.71443161139377 23.26488283135886 70-71 20.47422501983319 30.81125559712025 25.06547925017159 23.649040132874966 72-73 23.054511271214075 29.66067134793276 24.656871811899567 22.627945568953596 74-75 23.979959377216364 28.291649820982297 24.778709456840662 22.94968134496068 76-77 20.888116968151525 29.49102007678932 28.31127008403149 21.309592871027668 78-79 22.307344228677827 29.50156184924882 27.51829275199082 20.672801170082533 80-81 20.399763796196225 30.009321879677188 27.124014424773176 22.46689989935341 82-83 19.516883632963776 30.607558244796664 24.409567252743376 25.465990869496185 84-85 20.497184939799308 30.937530174622864 23.69469223741579 24.87059264816204 86-87 22.105222694747294 28.01837022984329 24.571194482765794 25.305212592643628 88-89 21.946395544207828 27.159673870236954 26.90824922029764 23.985681365257573 90-91 22.931437997917346 28.99839790011275 25.5744200279569 22.495744074013004 92-93 20.344603207145212 28.882654258811925 26.712783863846372 24.059958670196487 94-95 19.215124079512652 29.168262905392794 27.531919358298108 24.08469365679645 96-97 22.753676082924056 28.190944680168535 25.655631858543764 23.399747378363646 98-99 23.707099741731742 27.119076588899144 25.046082213787322 24.127741455581795 100-101 21.254625119635563 28.395511038021887 25.609276054755554 24.740587787587 102-103 22.992758766253345 28.082886844705456 25.464074481261395 23.460279907779803 104-105 23.531456171781713 27.04563694004544 25.529242470772374 23.89366441740047 106-107 21.063553214779258 28.2636179494765 27.96930521184838 22.70352362389586 108-109 21.73291265240759 29.082090132602094 27.29771651478264 21.887280700207672 110-111 20.4590627723649 29.921447526334987 26.661821530042168 22.957668171257943 112-113 19.719737632248922 30.586243606247894 24.560955394326626 25.133063367176554 114-115 20.040322408488905 32.010204416902 23.747656197356676 24.20181697725242 116-117 21.31195730237409 29.94113487943971 24.084797692902967 24.662110125283238 118-119 20.98548699571823 29.36049405276592 25.746134223561327 23.907884727954524 120-121 21.519496081617067 31.112235354073388 24.86657662817744 22.501691936132104 122-123 20.41875943043163 31.229357007924534 25.04204710166451 23.309836459979326 124-125 19.748441379033824 31.763587094733488 25.45925161690799 23.0287199093247 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15145.0 1 9256.5 2 5763.5 3 7969.0 4 6945.0 5 5348.0 6 4090.0 7 3331.5 8 3004.0 9 2836.0 10 2531.5 11 2253.0 12 2170.5 13 2134.5 14 2213.0 15 2334.0 16 2469.0 17 2769.0 18 3136.0 19 3567.5 20 4200.0 21 5212.5 22 6485.0 23 7983.0 24 9537.0 25 11757.0 26 15627.5 27 19749.5 28 22373.0 29 24883.5 30 28772.5 31 33112.0 32 37733.5 33 42791.0 34 48771.0 35 53001.0 36 56547.0 37 61219.5 38 61113.5 39 57495.5 40 55691.5 41 57858.0 42 60020.5 43 62652.0 44 65646.0 45 67023.5 46 70064.5 47 81264.5 48 105434.5 49 161118.0 50 303774.5 51 315089.0 52 160535.0 53 82215.0 54 59932.0 55 47309.0 56 39029.5 57 33381.0 58 30305.5 59 27016.0 60 23935.5 61 21855.5 62 19178.0 63 14618.5 64 10882.0 65 8730.0 66 6400.5 67 5301.0 68 3967.5 69 2458.5 70 2129.5 71 1965.5 72 1859.5 73 1801.5 74 1366.0 75 1016.0 76 679.0 77 338.0 78 184.5 79 91.0 80 54.5 81 29.5 82 17.5 83 15.0 84 13.0 85 12.0 86 10.0 87 5.0 88 6.0 89 7.5 90 5.5 91 6.5 92 6.0 93 4.0 94 3.5 95 5.0 96 5.0 97 3.0 98 2.5 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48439687412411064 2 0.47382149198674456 3 0.4591047014599204 4 0.45936353948426156 5 0.46173005856395183 6 0.46964310673666637 7 0.45537003853728414 8 0.48188244760193966 9 0.4933452743941896 10-11 0.4977640092382989 12-13 0.4978379629595392 14-15 0.4735256771017832 16-17 0.4753560317024812 18-19 0.4617115701336417 20-21 0.47086334313713163 22-23 0.4887416552469795 24-25 0.47542998542372156 26-27 0.48552466837302555 28-29 0.48123535254108685 30-31 0.49364108927915085 32-33 0.49181073467845293 34-35 0.4864121130279094 36-37 0.443629885290383 38-39 0.40607988333060935 40-41 0.3994055599886703 42-43 0.4084833792709198 44-45 0.4417440653987548 46-47 0.44919490281371727 48-49 0.4464771035581354 50-51 0.4327586882680556 52-53 0.4266390178354189 54-55 0.42856181458766734 56-57 0.4103322223019279 58-59 0.4159896819768126 60-61 0.4249011053862714 62-63 0.42591796905332585 64-65 0.44102301661666166 66-67 0.4300593774427839 68-69 0.43240740809216405 70-71 0.44142976208348345 72-73 0.4422432530171269 74-75 0.44603338123069347 76-77 0.4375102148577463 78-79 0.42865425673921775 80-81 0.4364748627603818 82-83 0.43527311479022657 84-85 0.43388648251697054 86-87 0.43948847690092496 88-89 0.4266944831263492 90-91 0.43181577832224155 92-93 0.44024650254363823 94-95 0.4387859165491419 96-97 0.4081505875253384 98-99 0.38071375694517884 100-101 0.37991875444184536 102-103 0.36076474064060193 104-105 0.36279846797471077 106-107 0.3816381784606829 108-109 0.3885343629663429 110-111 0.4056916262940977 112-113 0.4010695187165776 114-115 0.4013653336015388 116-117 0.3734293154030071 118-119 0.4455711704729415 120-121 0.45156142189340753 122-123 0.40622779077309 124-125 0.4003484699344844 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2704394.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.94187176953013 #Duplication Level Percentage of deduplicated Percentage of total 1 84.89942184954879 38.155389300696655 2 9.504147546560542 8.542683606324363 3 2.443456652332913 3.294405466306533 4 1.0115273294143194 1.8183972611965444 5 0.5050189758240253 1.134824902633139 6 0.3282728068212998 0.8851916633751953 7 0.21284162212137483 0.669585062201833 8 0.1472355293190585 0.5293632222860817 9 0.10984682783599078 0.4443049845805276 >10 0.6893014882435484 6.031509478284557 >50 0.08168441973403466 2.5704166760168743 >100 0.05802937335749701 5.076633166819377 >500 0.004607789443337608 1.4170279669997363 >1k 0.0015839276211473028 1.5173336412404814 >5k 8.639605206258015E-4 3.2730712204828363 >10k+ 0.002159901301564504 24.63986238055522 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 102545 3.791792172294422 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 50524 1.86821890597302 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 30470 1.1266849430963093 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 26884 0.9940859209124113 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 26538 0.9812919271378358 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 25371 0.9381399307941077 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 20827 0.7701170761360956 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 20235 0.7482267746489601 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 13257 0.49020224124147593 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 12281 0.4541128252761987 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 10707 0.39591124666006505 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 10677 0.3948019408414602 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 10222 0.37797746925928694 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 10117 0.37409489889417 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 10099 0.3734293154030071 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 9630 0.35608716777215155 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 8659 0.3201826361099751 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 8591 0.3176682095878041 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8189 0.30280351161849933 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 8112 0.29995629335074697 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 7397 0.27351783800733176 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 4598 0.17001960513150083 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3511 0.12982575763738569 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.6976860620161114E-5 0.0 8 0.0 0.0 0.0 3.6976860620161114E-5 0.0 9 0.0 0.0 0.0 3.6976860620161114E-5 0.0 10-11 0.0 0.0 0.0 2.218611637209667E-4 0.0 12-13 0.0 0.0 0.0 6.101182002326585E-4 0.0 14-15 0.0 0.0 0.0 0.0012387248307753973 0.0 16-17 0.0 0.0 0.0 0.0022371000675197473 0.0 18-19 0.0 0.0 0.0 0.0031060562920935337 0.0 20-21 0.0 0.0 0.0 0.004770015020000784 0.0 22-23 0.0 0.0 0.0 0.007876071312094318 0.0 24-25 0.0 0.0 0.0 0.013625973138529372 0.0 26-27 0.0 0.0 0.0 0.020651576656359985 0.0 28-29 0.0 0.0 0.0 0.041229199591479644 0.0 30-31 0.0 0.0 0.0 0.09950473192885356 0.0 32-33 0.0 0.0 0.0 0.20877135506142966 0.0 34-35 0.0 0.0 0.0 0.37483443610657324 0.0 36-37 0.0 0.0 0.0 0.5788357761480021 0.0 38-39 0.0 0.0 0.0 0.930393278494184 0.0 40-41 0.0 0.0 0.0 1.5001512353599364 0.0 42-43 0.0 0.0 0.0 1.9022930830344986 0.0 44-45 0.0 0.0 0.0 2.2787729894386692 0.0 46-47 0.0 0.0 0.0 2.725619861603006 0.0 48-49 0.0 0.0 0.0 3.117038419697722 0.0 50-51 0.0 0.0 0.0 3.5762355633091922 0.0 52-53 0.0 0.0 0.0 4.140724317536572 0.0 54-55 0.0 0.0 0.0 4.663059450656968 0.0 56-57 0.0 0.0 0.0 5.1877611028570545 0.0 58-59 0.0 0.0 0.0 5.7843272836724235 0.0 60-61 0.0 0.0 0.0 6.34796557010554 0.0 62-63 0.0 0.0 0.0 6.894668454374622 0.0 64-65 0.0 0.0 0.0 7.521278334443872 0.0 66-67 0.0 0.0 0.0 8.164620243943745 0.0 68-69 0.0 0.0 0.0 8.942927694707206 0.0 70-71 0.0 0.0 0.0 10.10010005938484 0.0 72-73 0.0 0.0 0.0 11.00457255858429 0.0 74-75 0.0 0.0 0.0 11.812017775516438 0.0 76-77 0.0 0.0 0.0 12.735662776947443 0.0 78-79 0.0 0.0 0.0 13.579123456123627 0.0 80-81 0.0 0.0 0.0 14.502602061681841 0.0 82-83 0.0 0.0 0.0 15.679094836033507 0.0 84-85 0.0 0.0 0.0 16.74402842189415 0.0 86-87 0.0 0.0 0.0 17.77459201580835 0.0 88-89 0.0 1.8488430310080557E-5 0.0 18.92089318346365 0.0 90-91 0.0 3.6976860620161114E-5 0.0 19.969797300245453 0.0 92-93 0.0 3.6976860620161114E-5 0.0 21.016501293820355 0.0 94-95 0.0 7.395372124032223E-5 0.0 22.20205339902396 0.0 96-97 0.0 7.395372124032223E-5 0.0 23.349482360928178 0.0 98-99 0.0 7.395372124032223E-5 0.0 24.624241142377922 0.0 100-101 0.0 7.395372124032223E-5 0.0 26.39328810816767 0.0 102-103 0.0 7.395372124032223E-5 0.0 27.814308861800463 0.0 104-105 0.0 7.395372124032223E-5 0.0 29.074535737026483 0.0 106-107 0.0 7.395372124032223E-5 0.0 30.419476599933294 0.0 108-109 0.0 7.395372124032223E-5 0.0 31.601848695123564 0.0 110-111 0.0 7.395372124032223E-5 0.0 32.82574580479027 0.0 112-113 0.0 7.395372124032223E-5 3.6976860620161114E-5 34.31913027465673 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 195 0.0 39.68325 2 TATCACG 125 2.9667717E-8 38.095924 2 ATAACGC 235 0.0 32.931717 3 ATCACGC 205 2.415618E-9 29.039225 3 CATGGGG 11280 0.0 28.129177 4 GTCGGGA 835 0.0 27.089167 2 CATTCCG 455 0.0 26.165268 9 GTACAAA 2125 0.0 26.04992 1 ATGGGGA 5865 0.0 25.883266 5 ATGGGGG 3900 0.0 24.728476 5 GAACAAA 2915 0.0 24.707521 1 GTACATA 1030 0.0 24.27138 1 ATGGGAG 4245 0.0 23.700415 5 TAGGACG 505 0.0 23.576399 4 CGGGAGT 945 0.0 23.308203 4 CGCAAAA 890 0.0 22.739841 2 AGTACTC 1480 0.0 22.1232 5 TATAGGG 300 5.7661964E-9 21.825792 2 ATGGGAT 4675 0.0 21.393145 5 TCGGGAG 1105 0.0 21.010733 3 >>END_MODULE