##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364164_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2704394 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98012456764806 33.0 31.0 34.0 30.0 34.0 2 31.77703507698952 33.0 31.0 34.0 30.0 34.0 3 32.017507064429225 33.0 31.0 34.0 30.0 34.0 4 35.49085747121166 37.0 35.0 37.0 33.0 37.0 5 35.653175905581804 37.0 35.0 37.0 33.0 37.0 6 35.794934465909925 37.0 35.0 37.0 33.0 37.0 7 35.76691118232033 37.0 35.0 37.0 33.0 37.0 8 35.82157148699487 37.0 35.0 37.0 33.0 37.0 9 37.451016382967865 39.0 37.0 39.0 34.0 39.0 10-11 37.41491476463858 39.0 37.0 39.0 34.0 39.0 12-13 37.39795625193666 39.0 37.0 39.0 34.0 39.0 14-15 38.60246029239822 40.0 38.0 41.0 34.0 41.0 16-17 38.60074068349508 40.0 38.0 41.0 34.0 41.0 18-19 38.72445157769171 40.0 38.0 41.0 34.0 41.0 20-21 38.78497493338618 40.0 38.0 41.0 34.0 41.0 22-23 38.71561836034247 40.0 38.0 41.0 34.0 41.0 24-25 38.66394097901416 40.0 38.0 41.0 34.0 41.0 26-27 38.45027388760661 40.0 38.0 41.0 34.0 41.0 28-29 38.275318980888144 40.0 38.0 41.0 34.0 41.0 30-31 38.049888810580114 40.0 37.0 41.0 34.0 41.0 32-33 37.6830001471679 40.0 36.5 41.0 33.0 41.0 34-35 37.423537768535205 40.0 36.0 41.0 33.0 41.0 36-37 37.24112241041801 40.0 36.0 41.0 33.0 41.0 38-39 37.0916367955261 40.0 35.5 41.0 32.5 41.0 40-41 36.840171587424024 40.0 35.0 41.0 31.0 41.0 42-43 36.598223853476966 40.0 35.0 41.0 31.0 41.0 44-45 36.23153264650047 40.0 35.0 41.0 30.0 41.0 46-47 35.714087518312795 40.0 35.0 41.0 28.0 41.0 48-49 35.234644988858875 39.5 35.0 41.0 24.0 41.0 50-51 34.99453056766137 39.0 35.0 41.0 23.0 41.0 52-53 34.86634214541225 39.0 35.0 41.0 23.0 41.0 54-55 34.68699309346197 39.0 35.0 41.0 22.0 41.0 56-57 34.43341317870103 39.0 35.0 41.0 20.0 41.0 58-59 34.29459242994919 39.0 35.0 41.0 19.0 41.0 60-61 34.1070738952978 39.0 34.5 40.5 18.5 41.0 62-63 33.795965935436925 38.0 34.0 40.0 17.0 41.0 64-65 33.52273596228952 38.0 34.0 40.0 12.5 41.0 66-67 33.082137255148474 37.0 34.0 40.0 8.0 41.0 68-69 32.74896205952239 36.5 33.5 39.0 7.0 41.0 70-71 32.33036883678932 36.0 33.0 39.0 2.0 40.5 72-73 31.997433805872962 35.0 33.0 38.5 2.0 40.0 74-75 31.64513565700856 35.0 33.0 37.0 2.0 39.0 76-77 31.208747504986327 35.0 33.0 37.0 2.0 39.0 78-79 30.8346967934406 35.0 32.0 36.0 2.0 38.0 80-81 30.58366643321942 35.0 32.0 36.0 2.0 37.0 82-83 30.35071609388277 35.0 32.0 36.0 2.0 37.0 84-85 30.100424531336778 35.0 32.0 35.0 2.0 36.0 86-87 29.87134696349718 35.0 32.0 35.0 2.0 36.0 88-89 29.71453419878908 35.0 31.5 35.0 2.0 36.0 90-91 29.55286618739725 35.0 31.0 35.0 2.0 35.5 92-93 29.388312132034017 35.0 31.0 35.0 2.0 35.0 94-95 29.305163744631884 35.0 31.0 35.0 2.0 35.0 96-97 29.215614847540706 35.0 31.0 35.0 2.0 35.0 98-99 29.121724497244113 35.0 31.0 35.0 2.0 35.0 100-101 28.972542277493588 35.0 31.0 35.0 2.0 35.0 102-103 27.74951930081194 33.5 28.0 34.5 2.0 35.0 104-105 28.560456427576753 34.0 30.0 35.0 2.0 35.0 106-107 28.641005896330196 34.0 30.5 35.0 2.0 35.0 108-109 28.561165643763445 35.0 30.0 35.0 2.0 35.0 110-111 28.463122976903513 35.0 30.0 35.0 2.0 35.0 112-113 28.317464467085788 34.5 30.0 35.0 2.0 35.0 114-115 28.082595768220163 34.0 30.0 35.0 2.0 35.0 116-117 27.81196656256448 34.0 29.0 35.0 2.0 35.0 118-119 27.595486086716654 34.0 29.0 35.0 2.0 35.0 120-121 27.348104787985775 34.0 29.0 35.0 2.0 35.0 122-123 27.019804251895252 34.0 27.0 35.0 2.0 35.0 124-125 25.999804947060227 33.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37990635372916515 1101 2 0.6793331066514732 1101 3 0.5115642133758023 1101 4 0.5752423490793745 1101 5 0.2895581477289326 1101 6 0.2730920208501786 1101 7 0.28371571881111635 1101 8 0.25837936778086856 1101 9 0.38467939924505146 1101 10-11 0.33554172379813707 1101 12-13 0.2859323840300192 1101 14-15 0.40069612506658814 1101 16-17 0.4598568400089462 1101 18-19 0.38134935041835405 1101 20-21 0.3034853918271878 1101 22-23 0.3728978744559228 1101 24-25 0.35572590974047813 1101 26-27 0.38093042361612817 1101 28-29 0.39856998085342354 1101 30-31 0.3879794540163104 1101 32-33 0.456377064403668 1101 34-35 0.5816720125069637 1101 36-37 0.5211225222516163 1101 38-39 0.4793552307934945 1101 40-41 0.44857654843517736 1101 42-43 0.5156327915222718 1101 44-45 0.6320649680188808 1101 46-47 0.7097033526455547 1101 48-49 0.7613323673753882 1101 50-51 0.7487004884344515 1101 52-53 0.8370372714351149 1101 54-55 0.8923551584303979 1101 56-57 0.8904020860749782 1101 58-59 0.8559657817989645 1101 60-61 0.8337517011086675 1101 62-63 0.867682090769307 1101 64-65 0.9106929458626638 1101 66-67 0.9385698005364418 1101 68-69 0.9293784642943592 1101 70-71 0.875526874559597 1101 72-73 0.8635225047993771 1101 74-75 0.7680035890290817 1101 76-77 0.7413860565803851 1101 78-79 0.6392575305182397 1101 80-81 0.6366051398887045 1101 82-83 0.6592059395410779 1101 84-85 0.6567133125808589 1101 86-87 0.6965285456459078 1101 88-89 0.6495938322087014 1101 90-91 0.7362171274043234 1101 92-93 0.6454185026152182 1101 94-95 0.6460745079353174 1101 96-97 0.6735302423916707 1101 98-99 0.6721905281952303 1101 100-101 0.6552882466667782 1101 102-103 0.577554904986286 1101 104-105 0.5826080891746095 1101 106-107 0.6287769887901931 1101 108-109 0.6369113186912898 1101 110-111 0.5663290738001479 1101 112-113 0.5746313310789759 1101 114-115 0.5554990753767903 1101 116-117 0.5487971890109407 1101 118-119 0.6364100336065768 1101 120-121 0.5566942685429339 1101 122-123 0.6433272134613759 1101 124-125 0.7012646340905384 1102 1 0.358112862970156 1102 2 0.6642062018694013 1102 3 0.5340064496111374 1102 4 0.6098431164387677 1102 5 0.4113026194734033 1102 6 0.29836646681427936 1102 7 0.301310519164538 1102 8 0.3006773307684867 1102 9 0.40029044244230505 1102 10-11 0.33565489115267866 1102 12-13 0.3457343714182173 1102 14-15 0.47544104463909775 1102 16-17 0.5108233013202366 1102 18-19 0.4171507810473756 1102 20-21 0.39652559952256894 1102 22-23 0.35644261489721174 1102 24-25 0.42640104076044594 1102 26-27 0.47073688928466595 1102 28-29 0.562381844665282 1102 30-31 0.5316378459505628 1102 32-33 0.4841475464845004 1102 34-35 0.5366804244119336 1102 36-37 0.6261493919336587 1102 38-39 0.627396285731102 1102 40-41 0.6706792826068764 1102 42-43 0.6004825875789663 1102 44-45 0.6228521165646512 1102 46-47 0.6678683470174462 1102 48-49 0.6647010629854577 1102 50-51 0.7050218232040635 1102 52-53 0.5783497101613477 1102 54-55 0.614766228255256 1102 56-57 0.7066388781738411 1102 58-59 0.7049170169571042 1102 60-61 0.6952424238062846 1102 62-63 0.578729826817046 1102 64-65 0.5861828334215176 1102 66-67 0.603439204198942 1102 68-69 0.6306989518217421 1102 70-71 0.6295343302911931 1102 72-73 0.6002931200182786 1102 74-75 0.6502154358961043 1102 76-77 0.5994145844364986 1102 78-79 0.6081749365046107 1102 80-81 0.6566442340657268 1102 82-83 0.6535811589162961 1102 84-85 0.6605679621251639 1102 86-87 0.6915482742863226 1102 88-89 0.7154998972526982 1102 90-91 0.6769543491760288 1102 92-93 0.6870056898920609 1102 94-95 0.8338763379785235 1102 96-97 0.8042684929575046 1102 98-99 0.7992417621430192 1102 100-101 0.8100095039742428 1102 102-103 0.7037047337223221 1102 104-105 0.7762788871557511 1102 106-107 0.7651515182681692 1102 108-109 0.7569976163637975 1102 110-111 0.6841402235078533 1102 112-113 0.6460453783550939 1102 114-115 0.7199799579611188 1102 116-117 0.6161665624147972 1102 118-119 0.6095963300687366 1102 120-121 0.6164327127641371 1102 122-123 0.6138213977486622 1102 124-125 0.6474620294258635 1103 1 0.2181563300464937 1103 2 0.5627305330942924 1103 3 0.4238816019742977 1103 4 0.5324552521195116 1103 5 0.24880872001025267 1103 6 0.20190610046952884 1103 7 0.2033795327648278 1103 8 0.15270751730668053 1103 9 0.2742678963006071 1103 10-11 0.2616309169444264 1103 12-13 0.1877066659653508 1103 14-15 0.32812407877565875 1103 16-17 0.37904016548802844 1103 18-19 0.27376256091356055 1103 20-21 0.20085457653166117 1103 22-23 0.29543652517904917 1103 24-25 0.2643801582922478 1103 26-27 0.1538147814199533 1103 28-29 0.2413193054475471 1103 30-31 0.1972989758541317 1103 32-33 0.18783269146690884 1103 34-35 0.22563598846216593 1103 36-37 0.2286028896148551 1103 38-39 0.21019205057613988 1103 40-41 0.1854814088393013 1103 42-43 0.19235985800043665 1103 44-45 0.18219471309738822 1103 46-47 0.1947888441000103 1103 48-49 0.15271954851366587 1103 50-51 0.16901260808771212 1103 52-53 0.06365030134244165 1103 54-55 0.1844808192969083 1103 56-57 0.16671251736344317 1103 58-59 0.13159349256577002 1103 60-61 0.06541921315685073 1103 62-63 0.06917290009319998 1103 64-65 0.1475965471879377 1103 66-67 0.15242158656977978 1103 68-69 0.2393317824467971 1103 70-71 0.16092054055356186 1103 72-73 0.17618518078563028 1103 74-75 0.15731928566542663 1103 76-77 0.15366807105859337 1103 78-79 0.1375364296756132 1103 80-81 0.026013528711438028 1103 82-83 0.01985564821421093 1103 84-85 0.013589921653665016 1103 86-87 0.038333261315923295 1103 88-89 0.08087246875820142 1103 90-91 0.11391338482239632 1103 92-93 0.02754072199951807 1103 94-95 0.07881457530203306 1103 96-97 0.10880783330615529 1103 98-99 0.1224056508115261 1103 100-101 0.1938750565566636 1103 102-103 0.1300265367404947 1103 104-105 0.20602203952852705 1103 106-107 0.2259937649967192 1103 108-109 0.13878027136551196 1103 110-111 0.2060369435226619 1103 112-113 0.16061598470638216 1103 114-115 0.15195620733904747 1103 116-117 0.19583109900239393 1103 118-119 0.2618171057925629 1103 120-121 0.33624365021530167 1103 122-123 0.3233228486546693 1103 124-125 0.3430723475673574 1104 1 0.28324432477797856 1104 2 0.5929046409458998 1104 3 0.10411268737005841 1104 4 0.5596199577943324 1104 5 0.22178454718424945 1104 6 0.20395611605162145 1104 7 0.22914243797277578 1104 8 0.1625148285428395 1104 9 0.29334284911332276 1104 10-11 0.20031866941845777 1104 12-13 0.22222911888097485 1104 14-15 0.2700419111593675 1104 16-17 0.35843740304733984 1104 18-19 0.28271550298932624 1104 20-21 0.17759480244261994 1104 22-23 0.21614289384551455 1104 24-25 0.25150223370958 1104 26-27 0.19456560315492055 1104 28-29 0.3861339961282795 1104 30-31 0.36447955762483986 1104 32-33 0.3633685685036028 1104 34-35 0.33391942969414146 1104 36-37 0.4173218650774615 1104 38-39 0.38673511889627576 1104 40-41 0.4058303301467845 1104 42-43 0.4376999298544746 1104 44-45 0.35428988735223754 1104 46-47 0.4196553966939831 1104 48-49 0.3603889376367775 1104 50-51 0.2815156804062724 1104 52-53 0.3153919678621051 1104 54-55 0.3070752673673738 1104 56-57 0.4235656987566614 1104 58-59 0.47923418711556565 1104 60-61 0.40514479189934605 1104 62-63 0.43434944815955845 1104 64-65 0.4973407656430169 1104 66-67 0.43604974488385295 1104 68-69 0.46892317877883016 1104 70-71 0.39006611259281243 1104 72-73 0.3207973042428591 1104 74-75 0.3766202670433536 1104 76-77 0.35770736145590476 1104 78-79 0.33397142294297666 1104 80-81 0.29687748232971956 1104 82-83 0.2180475960935766 1104 84-85 0.2230508987979647 1104 86-87 0.2602054646240326 1104 88-89 0.2632976894065351 1104 90-91 0.27690508809228476 1104 92-93 0.23065902243405034 1104 94-95 0.2699772935248461 1104 96-97 0.32368003073422713 1104 98-99 0.30806424418943124 1104 100-101 0.33303609308932636 1104 102-103 0.23638708875823156 1104 104-105 0.31901109678243245 1104 106-107 0.40324814121315455 1104 108-109 0.38271783714841234 1104 110-111 0.3542794009071031 1104 112-113 0.30137151046976385 1104 114-115 0.23686315577701222 1104 116-117 0.17574724969594158 1104 118-119 0.26036403141599607 1104 120-121 0.31324736051771396 1104 122-123 0.1614835735454463 1104 124-125 0.17415827252634486 1105 1 0.20627206324306613 1105 2 0.5226763489075061 1105 3 0.14135404704178356 1105 4 0.40442841402531116 1105 5 0.15662287473220715 1105 6 0.07931054250240521 1105 7 0.08919864958357238 1105 8 0.1251369549591601 1105 9 0.16488940878618763 1105 10-11 0.12469386341116007 1105 12-13 0.12157012744370377 1105 14-15 0.2334953657244938 1105 16-17 0.27400864571121275 1105 18-19 0.22104532902948648 1105 20-21 0.13580252342345034 1105 22-23 0.15932295039883826 1105 24-25 0.146189012021118 1105 26-27 0.20008914852999737 1105 28-29 0.2421350269592324 1105 30-31 0.20268650975385327 1105 32-33 0.13479934478765898 1105 34-35 0.13264581241718787 1105 36-37 0.23589766674786716 1105 38-39 0.2126803083043427 1105 40-41 0.39148193206379034 1105 42-43 0.30482169035428086 1105 44-45 0.4151203327452464 1105 46-47 0.4347534537769562 1105 48-49 0.40425409811323476 1105 50-51 0.3645477187463442 1105 52-53 0.28744406184426907 1105 54-55 0.36603230563626 1105 56-57 0.3883850328170837 1105 58-59 0.3862195191314086 1105 60-61 0.3856655323890834 1105 62-63 0.36146693726036716 1105 64-65 0.3543890011280979 1105 66-67 0.3382617315684797 1105 68-69 0.35754793068173285 1105 70-71 0.2881949550721643 1105 72-73 0.280457500214645 1105 74-75 0.25740099335203936 1105 76-77 0.17108524073477938 1105 78-79 0.2345529312024368 1105 80-81 0.2547981368219503 1105 82-83 0.2645993241592315 1105 84-85 0.28774308754823963 1105 86-87 0.28031261978687283 1105 88-89 0.26810816743303434 1105 90-91 0.2581908361423899 1105 92-93 0.23795263555350843 1105 94-95 0.26566693936891994 1105 96-97 0.2959394868997087 1105 98-99 0.30384239342072306 1105 100-101 0.28651970373137203 1105 102-103 0.20023046509980347 1105 104-105 0.25114144279422845 1105 106-107 0.24231811527152303 1105 108-109 0.35459958773857636 1105 110-111 0.39958513340931745 1105 112-113 0.4522251353213278 1105 114-115 0.36541224696568975 1105 116-117 0.4185404029281088 1105 118-119 0.3926507675830706 1105 120-121 0.4234011593576028 1105 122-123 0.39966783030778075 1105 124-125 0.3750677672767928 1106 1 0.19631389157354917 1106 2 0.5149468305618363 1106 3 0.3833303226853637 1106 4 0.496044590275595 1106 5 0.1953698268893973 1106 6 0.11201458399058595 1106 7 0.13060916234418585 1106 8 0.03592783165610314 1106 9 0.24624823659604544 1106 10-11 0.20628175699471996 1106 12-13 0.16333593316010564 1106 14-15 0.29325066738937977 1106 16-17 0.3222244166870709 1106 18-19 0.17719678850217946 1106 20-21 0.15963615440900725 1106 22-23 0.12285260639210804 1106 24-25 0.14203986511585498 1106 26-27 0.12628404121771553 1106 28-29 0.2006288236422762 1106 30-31 0.18271867865124847 1106 32-33 0.22043735349291893 1106 34-35 0.1223587489642739 1106 36-37 0.027374497391186026 1106 38-39 0.05666838614836678 1106 40-41 0.041494516440046425 1106 42-43 0.13480794476463132 1106 44-45 0.19496238033691782 1106 46-47 0.24361482653385025 1106 48-49 0.14509038537442365 1106 50-51 0.06410415714055517 1106 52-53 0.10949421695115547 1106 54-55 0.1319891988755657 1106 56-57 0.21240122626647207 1106 58-59 0.17452212915021903 1106 60-61 0.15513474010468542 1106 62-63 0.13264887995394048 1106 64-65 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-1.9756354885192309 2216 120-121 -2.122716226211004 2216 122-123 -2.1646443458036693 2216 124-125 -2.5601410299467062 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35.0 3 36.0 4 128.0 5 469.0 6 1554.0 7 2809.0 8 4732.0 9 9623.0 10 19634.0 11 25975.0 12 24912.0 13 31971.0 14 47253.0 15 44830.0 16 20457.0 17 15777.0 18 15803.0 19 15986.0 20 16084.0 21 14911.0 22 15009.0 23 15666.0 24 17280.0 25 19726.0 26 23697.0 27 28736.0 28 36505.0 29 44553.0 30 54801.0 31 69402.0 32 89290.0 33 115791.0 34 160708.0 35 241536.0 36 413023.0 37 723156.0 38 321280.0 39 1256.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.7390946270421 18.567129756496417 12.637590115878613 23.056185500582874 2 12.961680410385052 23.80365679911936 37.1496756936045 26.084987096891087 3 21.58265187833833 28.533622246832458 30.527901907457704 19.35582396737151 4 13.43942487670066 19.00085564455475 33.76272096447485 33.79699851426974 5 10.795061666310456 39.27567506805591 35.49615921348738 14.433104052146248 6 30.548427999480825 32.31070006097712 19.247285704618545 17.893586234923507 7 21.89340754342747 34.4245328158545 24.04982410107403 19.632235539644004 8 28.0863661138133 31.200261500358305 23.450170352396878 17.26320203343152 9 28.65651232771556 13.998810824162454 21.69147690758077 35.65319994054121 10-11 23.386244814451757 27.512626687487128 26.798880043603162 22.302248454457953 12-13 27.298538328771098 25.506130588042787 27.542634003477833 19.652697079708275 14-15 26.54204166393958 23.46132328139115 28.192140597030047 21.80449445763922 16-17 20.790144136548857 27.50072105251481 32.31853511710632 19.39059969383001 18-19 22.136900170611234 24.370820228117648 35.788923507447514 17.703356093823608 20-21 20.44155227554004 24.191121032424675 35.46635823000447 19.900968462030825 22-23 18.962658547534126 25.652327286630573 32.32798179555198 23.057032370283324 24-25 20.41657021868855 26.396301722308213 31.080123680203403 22.107004378799836 26-27 21.556640785329357 23.511514964165727 32.56474463410287 22.367099616402047 28-29 21.0042676930478 22.240739449971777 35.780657993479856 20.974334863500566 30-31 22.69820891482528 24.379361882920904 33.69523819384306 19.227191008410756 32-33 18.22482227071943 24.727887282696233 35.63661581855306 21.41067462803127 34-35 17.022806415858643 25.76197796732538 36.17805107801475 21.03716453880123 36-37 21.946802130162986 24.464112847462317 32.892378107627806 20.69670691474689 38-39 22.961206835986175 22.710522209411792 33.02656713481837 21.301703819783654 40-41 20.137938531862787 24.87854021216591 33.233391004931605 21.7501302510397 42-43 22.92561660763927 24.025234488761622 32.576503275780084 20.472645627819023 44-45 23.973189557438744 22.346374086024447 32.35042674994842 21.33000960658839 46-47 20.76113909437752 24.6878413426446 35.09292654842452 19.458093014553352 48-49 22.531369319707114 24.567093404289466 33.7712441308478 19.130293145155626 50-51 20.078435316747488 25.23435934261058 32.686583389846305 22.000621950795633 52-53 19.050663475810108 26.520617927713197 29.764024768580317 24.66469382789638 54-55 20.576661536743536 26.55343489151359 28.536947648900274 24.332955922842604 56-57 21.760253868334274 24.242270172171658 29.57414858929579 24.42332737019828 58-59 21.38288651727522 22.765247963129635 32.38566569811943 23.466199821475715 60-61 22.715292224431796 25.01785982367954 30.540169072997497 21.726678878891168 62-63 18.912018736914813 25.475929173042093 32.019225009373635 23.59282708066946 64-65 17.63287819748158 26.09063250399165 32.68268233105088 23.593806967475892 66-67 21.657735522264876 25.500980996112254 29.883053282916617 22.958230198706254 68-69 22.767984250815523 23.676468739392263 29.983815228106554 23.571731781685656 70-71 20.27933060049682 25.41351223231526 30.283882452039162 24.023274715148755 72-73 22.588849849541155 24.757820051368252 29.823354141445368 22.829975957645225 74-75 23.268318151866925 23.39598076315803 29.928664240491585 23.407036844483457 76-77 20.465435139998092 24.941835398244486 32.75266103977453 21.840068421982892 78-79 21.42189710522949 24.901844923483782 32.07237924651511 21.603878724771615 80-81 19.528238119149798 25.36216986134417 31.56145147489604 23.548140544609993 82-83 18.682521851475784 26.092740185047003 28.993778273432053 26.230959690045164 84-85 19.79924153063496 25.928858738778448 28.13340068052214 26.13849905006445 86-87 20.808913198298768 23.737055325518398 29.141371412597422 26.312660063585408 88-89 20.569377095201364 22.745650227000947 31.350701857791428 25.334270820006253 90-91 21.34130603750785 24.436934854906497 29.959743291842834 24.262015815742824 92-93 18.66554947245113 24.633559311254203 30.955567125204393 25.74532409109028 94-95 17.70725541155635 24.95717586451137 31.597177092151995 25.73839163178029 96-97 20.7518525914093 24.311361081528812 29.477558129215474 25.459228197846407 98-99 21.375390005685414 23.12973380234165 29.49801010432508 25.996866087647856 100-101 19.63441717442059 24.24295424409313 29.856208082106377 26.2664204993799 102-103 21.10521350988666 23.8089436050203 29.639267226540237 25.4465756585528 104-105 21.578578200769915 22.8595130605685 29.892560728735578 25.669348009926008 106-107 19.70213554402673 24.450913996866806 31.37889486040412 24.468055598702342 108-109 20.120963700534308 25.034866703971236 30.50868958518331 24.33548001031115 110-111 18.64181311015552 25.403753181661425 30.430930787942557 25.5235029202405 112-113 18.1054379868025 26.01461835941386 28.438973859808275 27.440969793975363 114-115 18.510585840403845 26.381434277936506 27.648975540861194 27.459004340798455 116-117 19.370778461853174 24.859520194486457 28.322201052814783 27.44750029084559 118-119 19.305731798561364 24.346265326977367 29.57057649369723 26.77742638076404 120-121 19.67216479480807 25.738362373506472 28.576059206191918 26.01341362549354 122-123 17.989717921382738 26.178181034250052 28.941207706049255 26.890893338317955 124-125 17.33161215222395 26.825095693244705 29.14700771295965 26.6962844415717 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4060.0 1 3024.0 2 4165.5 3 5338.0 4 2969.5 5 1978.5 6 3549.5 7 6984.0 8 9954.0 9 10074.5 10 7567.0 11 5122.5 12 4673.5 13 4906.5 14 5395.5 15 6118.5 16 6775.5 17 7637.0 18 8506.5 19 8830.5 20 9183.5 21 10253.0 22 11505.0 23 12586.5 24 13756.5 25 15573.0 26 18584.5 27 21822.5 28 23668.5 29 25703.0 30 29097.5 31 32450.0 32 36290.0 33 40504.0 34 45480.0 35 49597.5 36 52940.0 37 57012.5 38 56299.5 39 52245.0 40 51163.5 41 53416.5 42 55722.0 43 59022.5 44 62019.5 45 63739.0 46 66977.5 47 79968.0 48 104465.5 49 164234.0 50 327980.0 51 334867.5 52 152546.5 53 65922.0 54 48356.5 55 42278.5 56 35897.5 57 30663.5 58 28048.5 59 25528.5 60 22686.5 61 20337.5 62 17854.5 63 13923.0 64 10564.0 65 8377.5 66 5875.0 67 4910.5 68 3778.0 69 2342.0 70 2008.5 71 1871.0 72 1743.5 73 1621.0 74 1340.0 75 1090.0 76 621.5 77 248.0 78 119.0 79 61.0 80 30.5 81 9.5 82 3.5 83 2.5 84 3.0 85 1.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14724185898948156 2 2.588380243411278E-4 3 3.3279174558145E-4 4 0.0 5 0.0 6 0.003734662922636273 7 0.0 8 0.0 9 0.0 10-11 1.1093058186048335E-4 12-13 0.004677572868450381 14-15 0.0015160512854266058 16-17 5.176760486822556E-4 18-19 0.0 20-21 1.1093058186048335E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.0337273341088614E-4 30-31 0.0 32-33 0.0 34-35 5.5465290930241674E-5 36-37 0.0 38-39 0.0 40-41 3.6976860620161114E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 7.210487820931418E-4 96-97 0.0027362876858919223 98-99 0.003938035656047159 100-101 0.0 102-103 0.003438848037674984 104-105 0.021723905614344657 106-107 0.016491679836591857 108-109 0.019412851825584585 110-111 0.01558574675139791 112-113 0.014679813666203963 114-115 0.04028628964566554 116-117 0.03954675243326231 118-119 0.021557509741553932 120-121 0.013034343368606793 122-123 0.002847218267752406 124-125 0.0018303546006979753 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2704394.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.77594595490473 #Duplication Level Percentage of deduplicated Percentage of total 1 85.18792959438858 36.43994272339795 2 9.279540350106453 7.938822330050227 3 2.4101088780459263 3.092840613381859 4 0.9642646504107707 1.649893302887849 5 0.5285674186320598 1.130498566646425 6 0.3059715975756879 0.7852934712980091 7 0.20468103984280514 0.6128797568816674 8 0.1538904752735789 0.526624852262177 9 0.11425946524921068 0.4398801039302896 >10 0.6751822737323488 5.693622064140134 >50 0.08881699809534137 2.6485972543466696 >100 0.07140545915586753 5.981018869339784 >500 0.006914753899851668 2.095969714332705 >1k 0.005080227354993062 3.8630746454246823 >5k 0.0011289394122206806 3.632543329860008 >10k+ 0.002257878824441361 23.468498401819694 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 88485 3.2718975119749563 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 48902 1.808242438047119 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 29731 1.0993590430980102 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 28480 1.0531009904621884 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 26735 0.9885763686800074 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21780 0.8053560243071091 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 20905 0.7730012712644682 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 18859 0.6973466144356185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18348 0.6784514386587162 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14705 0.5437447354194692 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 14646 0.5415631006428797 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 13910 0.5143481312264412 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 11250 0.4159896819768126 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 11070 0.40933384706518355 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 10662 0.3942472879321578 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 10313 0.3813423635757216 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 9956 0.36814162433432407 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 9448 0.3493573791392822 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9131 0.3376357143226912 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 8363 0.3092374853664074 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 6617 0.2446758867236061 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5796 0.21431788415445385 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5659 0.20925205424949175 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 5207 0.19253851324917892 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4645 0.1717575175806484 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3859 0.14269370513320176 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT 3777 0.13966160256234852 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3632 0.13429995777242518 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.6976860620161114E-5 0.0 8 0.0 0.0 0.0 3.6976860620161114E-5 0.0 9 0.0 0.0 0.0 3.6976860620161114E-5 0.0 10-11 0.0 0.0 0.0 2.218611637209667E-4 0.0 12-13 0.0 0.0 0.0 5.731413396124973E-4 0.0 14-15 0.0 0.0 0.0 0.001257213261085478 0.0 16-17 0.0 0.0 0.0 0.0023665190796903113 0.0 18-19 0.0 0.0 0.0 0.0032354753042640977 0.0 20-21 0.0 0.0 0.0 0.00497338775341167 0.0 22-23 0.0 0.0 0.0 0.008116420906125365 0.0 24-25 0.0 0.0 0.0 0.01384783430225034 0.0 26-27 0.0 0.0 0.0 0.021095298983801913 0.0 28-29 0.0 0.0 0.0 0.041950248373572786 0.0 30-31 0.0 0.0 0.0 0.10065101460807856 0.0 32-33 0.0 0.0 0.0 0.2111933394320502 0.0 34-35 0.0 0.0 0.0 0.37936410153254296 0.0 36-37 0.0 0.0 0.0 0.5846041664047472 0.0 38-39 0.0 0.0 0.0 0.9385836531215497 0.0 40-41 0.0 0.0 0.0 1.5131486018679232 0.0 42-43 0.0 0.0 0.0 1.9179342950768268 0.0 44-45 0.0 0.0 0.0 2.2964849056757264 0.0 46-47 0.0 0.0 0.0 2.746511787853397 0.0 48-49 0.0 0.0 0.0 3.1406296567733847 0.0 50-51 0.0 0.0 0.0 3.6026000649313676 0.0 52-53 0.0 0.0 0.0 4.170453713475181 0.0 54-55 0.0 0.0 0.0 4.6967823475425545 0.0 56-57 0.0 0.0 0.0 5.225459012259309 0.0 58-59 0.0 0.0 0.0 5.8280154444951435 0.0 60-61 0.0 0.0 0.0 6.397052352578804 0.0 62-63 0.0 0.0 0.0 6.949061416346878 0.0 64-65 0.0 0.0 0.0 7.581180848648533 0.0 66-67 0.0 0.0 3.6976860620161114E-5 8.228516259095382 0.0 68-69 0.0 0.0 3.6976860620161114E-5 9.01285093813993 0.0 70-71 0.0 0.0 3.6976860620161114E-5 10.177363209650665 0.0 72-73 0.0 0.0 3.6976860620161114E-5 11.08775200654934 0.0 74-75 0.0 0.0 3.6976860620161114E-5 11.900152122804592 0.0 76-77 0.0 0.0 3.6976860620161114E-5 12.830009236819784 0.0 78-79 0.0 0.0 3.6976860620161114E-5 13.680384588931938 0.0 80-81 0.0 0.0 3.6976860620161114E-5 14.610777867426123 0.0 82-83 0.0 0.0 3.6976860620161114E-5 15.79710648670275 0.0 84-85 0.0 0.0 3.6976860620161114E-5 16.870840565390992 0.0 86-87 0.0 0.0 3.6976860620161114E-5 17.911387911672634 0.0 88-89 0.0 0.0 3.6976860620161114E-5 19.06698875977391 0.0 90-91 0.0 0.0 3.6976860620161114E-5 20.12770698352385 0.0 92-93 0.0 0.0 3.6976860620161114E-5 21.18622508406689 0.0 94-95 0.0 0.0 3.6976860620161114E-5 22.385107347524066 0.0 96-97 0.0 0.0 3.6976860620161114E-5 23.546125305706195 0.0 98-99 0.0 0.0 3.6976860620161114E-5 24.83903972572044 0.0 100-101 0.0 0.0 3.6976860620161114E-5 26.627092797868947 0.0 102-103 0.0 0.0 3.6976860620161114E-5 28.06382871726531 0.0 104-105 0.0 0.0 3.6976860620161114E-5 29.341989369892108 0.0 106-107 0.0 0.0 3.6976860620161114E-5 30.711168564935434 0.0 108-109 0.0 0.0 3.6976860620161114E-5 31.915061193006643 0.0 110-111 0.0 0.0 3.6976860620161114E-5 33.16426896376785 0.0 112-113 0.0 0.0 3.6976860620161114E-5 34.68446165758392 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 160 0.0 52.058163 2 TATAACG 130 0.0 50.34196 2 ATAACGC 170 0.0 45.496212 3 GTTATCA 1565 0.0 44.53801 1 ACCGTCC 165 2.3646862E-10 36.058937 8 CGCATGT 175 4.4019544E-10 34.042484 1 TTATCAA 2115 0.0 32.06825 2 ATCACGC 280 0.0 31.872345 3 TACCGTC 75 0.0039123013 31.731865 7 GTACCGT 80 0.005364201 29.74917 6 TCACGCA 305 0.0 29.259857 4 AGTACTC 1455 0.0 29.031944 5 TAACGCA 270 1.715307E-9 24.238722 4 CACGCAG 380 0.0 23.484886 5 GCAACGC 205 2.266639E-6 23.217579 4 AAGTACT 1870 0.0 22.589027 4 ATACGCA 135 0.0025574046 22.035202 4 GTGTAGC 670 0.0 21.340065 1 ACAACGC 340 1.0732037E-9 20.998251 3 GTGTTAT 655 0.0 20.919237 1 >>END_MODULE