FastQCFastQC Report
Thu 26 May 2016
SRR1364163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2650017
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA661932.4978330327692237No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT509711.9234216233329822No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG340681.2855766585648318No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG160090.6041093321288127No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC156430.5902980999744529No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG126700.47811014042551425No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT109620.41365772370516873No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN95640.3609033451483519No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC76100.2871679691111415No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT73900.27886613557573403No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA64430.24313051576650263No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG63930.24124373541754637No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG60980.2301117313587045No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC57040.21524390220892922No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG51720.19516855929603472No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT47270.17837621419032407No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG45350.17113097765033206No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA43060.16248952365211242No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42320.1596970887356572No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC31760.11984828776570111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG79300.027.1013534
ATGGGAG45550.025.3582765
AGTACTC15550.024.887965
ATGGGAT48800.024.7675235
ATGGGGG34900.023.2017025
CGTATAG1552.0996682E-423.0445521
ATGGGGA43250.019.9612735
CATGGGT46200.018.8134234
GTATAGG7600.018.7995011
CGCTAGA1600.006815430718.6029662
TCGCGTG1000.001177388617.84480554-55
GTCGGGA8150.017.5301592
GTATTAG8850.017.4895551
CGTCAAT2050.001381435817.423931
ATGGGTA27400.017.1665445
CGCGTAC1454.5264445E-516.41212786-87
CGGGAGT9950.016.1546544
GATATAG8550.016.014391
AAGTACT22350.015.9819834
GTACACG4553.9295628E-815.7006831
Produced by FastQC (version 0.11.5)