##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364163_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2650017 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.033047335168035 33.0 31.0 34.0 30.0 34.0 2 31.861362398807252 33.0 31.0 34.0 30.0 34.0 3 32.07955835755016 34.0 31.0 34.0 30.0 34.0 4 35.5762981897852 37.0 35.0 37.0 33.0 37.0 5 35.71388523167965 37.0 35.0 37.0 33.0 37.0 6 35.83837613117199 37.0 35.0 37.0 35.0 37.0 7 35.81309327449598 37.0 35.0 37.0 35.0 37.0 8 35.8604299519588 37.0 35.0 37.0 35.0 37.0 9 37.51244312772333 39.0 37.0 39.0 35.0 39.0 10-11 37.47763467177758 39.0 37.0 39.0 34.0 39.0 12-13 37.457591781486684 39.0 37.0 39.0 34.0 39.0 14-15 38.73094097132207 40.0 38.0 41.0 34.0 41.0 16-17 38.71697521185713 40.0 38.0 41.0 34.0 41.0 18-19 38.825261875678535 40.0 38.0 41.0 34.5 41.0 20-21 38.89875498911894 40.0 38.0 41.0 35.0 41.0 22-23 38.86308767075834 40.0 38.0 41.0 35.0 41.0 24-25 38.82939166050633 40.0 38.0 41.0 34.5 41.0 26-27 38.67808659340676 40.0 38.0 41.0 34.0 41.0 28-29 38.54118049054025 40.0 38.0 41.0 34.0 41.0 30-31 38.4141039472577 40.0 38.0 41.0 34.0 41.0 32-33 38.18910218311807 40.0 37.0 41.0 34.0 41.0 34-35 38.030750934805326 40.0 37.0 41.0 33.5 41.0 36-37 37.932503074508574 40.0 37.0 41.0 33.0 41.0 38-39 37.8400442336785 40.0 37.0 41.0 33.0 41.0 40-41 37.66364178041122 40.0 37.0 41.0 33.0 41.0 42-43 37.50632241227132 40.0 37.0 41.0 33.0 41.0 44-45 37.266859231469084 40.0 36.5 41.0 32.5 41.0 46-47 36.9126792394162 40.0 36.0 41.0 31.0 41.0 48-49 36.568377485880276 40.0 36.0 41.0 30.5 41.0 50-51 36.371288938901145 40.0 36.0 41.0 30.0 41.0 52-53 36.24801822026048 40.0 35.5 41.0 30.0 41.0 54-55 36.07990344967598 39.0 35.0 41.0 29.0 41.0 56-57 35.85841109698542 39.0 35.0 41.0 28.5 41.0 58-59 35.721753671768894 39.0 35.0 41.0 28.0 41.0 60-61 35.58332116360008 39.0 35.0 41.0 28.0 41.0 62-63 35.307886892801065 38.0 35.0 40.0 28.0 41.0 64-65 35.040325401686104 38.0 35.0 40.0 28.0 41.0 66-67 34.67955752736681 37.0 34.5 40.0 28.0 41.0 68-69 34.3716698798536 37.0 34.0 39.5 27.5 41.0 70-71 34.03531939606425 36.0 34.0 39.0 27.0 41.0 72-73 33.7651345255521 36.0 34.0 39.0 27.5 40.5 74-75 33.425427459521956 35.0 34.0 37.5 26.0 39.5 76-77 33.02894604072351 35.0 34.0 37.0 26.0 39.0 78-79 32.6851357934685 35.0 34.0 36.5 26.0 39.0 80-81 32.42666348932856 35.0 33.5 36.0 26.0 38.0 82-83 32.18938387942417 35.0 33.0 36.0 26.0 37.0 84-85 31.94974786954197 35.0 33.0 35.0 25.0 37.0 86-87 31.714135230075883 35.0 33.0 35.0 25.0 36.0 88-89 31.53836899914227 35.0 33.0 35.0 24.5 36.0 90-91 31.389600330865804 35.0 33.0 35.0 24.0 36.0 92-93 31.24657709742994 35.0 33.0 35.0 24.0 36.0 94-95 31.165312335732185 35.0 33.0 35.0 23.0 35.0 96-97 31.093867322360573 35.0 33.0 35.0 23.0 35.0 98-99 31.012152186193525 35.0 33.0 35.0 21.5 35.0 100-101 30.873472509798994 35.0 33.0 35.0 20.0 35.0 102-103 29.65541956900654 33.5 30.0 34.5 17.5 35.0 104-105 30.506904295330934 34.5 32.0 35.0 18.0 35.0 106-107 30.61097777863312 35.0 32.0 35.0 18.0 35.0 108-109 30.584824550182134 35.0 32.0 35.0 17.5 35.0 110-111 30.555082854185464 35.0 32.5 35.0 16.0 35.0 112-113 30.48341576676678 35.0 32.0 35.0 8.5 35.0 114-115 30.38222169895514 35.0 32.0 35.0 4.5 35.0 116-117 30.218651804875215 35.0 32.0 35.0 2.0 35.0 118-119 30.0892856536392 35.0 32.0 35.0 2.0 35.0 120-121 29.938666242518444 35.0 32.0 35.0 2.0 35.0 122-123 29.702809831031274 35.0 32.0 35.0 2.0 35.0 124-125 28.743053157772195 34.0 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33133203523670574 1101 2 0.6233708821777704 1101 3 0.5092438852973444 1101 4 0.5709972800861323 1101 5 0.2557043033747064 1101 6 0.2680574214652154 1101 7 0.3014899730381728 1101 8 0.24705904859563788 1101 9 0.34756933611043905 1101 10-11 0.320185907145202 1101 12-13 0.35643593606697976 1101 14-15 0.4139664047107985 1101 16-17 0.4049488227372393 1101 18-19 0.3800582743437104 1101 20-21 0.278550479597115 1101 22-23 0.33720629409042147 1101 24-25 0.31942189115638797 1101 26-27 0.365977443663958 1101 28-29 0.45561403061584826 1101 30-31 0.4547163054013268 1101 32-33 0.4505078346082385 1101 34-35 0.4467470243174603 1101 36-37 0.4941170384479108 1101 38-39 0.4238242488234505 1101 40-41 0.6010512225681808 1101 42-43 0.537663334386302 1101 44-45 0.5576847741685071 1101 46-47 0.4940349528151913 1101 48-49 0.5374050347999741 1101 50-51 0.5739046687930269 1101 52-53 0.5861799493038262 1101 54-55 0.5570552572338485 1101 56-57 0.5890439307137854 1101 58-59 0.5136286784961754 1101 60-61 0.5224826191378185 1101 62-63 0.5609363056416043 1101 64-65 0.4928827462766776 1101 66-67 0.503336415257543 1101 68-69 0.5512675576766952 1101 70-71 0.5537937483647184 1101 72-73 0.5333317632269399 1101 74-75 0.5068677341047518 1101 76-77 0.49101403390087484 1101 78-79 0.4641830612164952 1101 80-81 0.4300241323335001 1101 82-83 0.4562225402725062 1101 84-85 0.4930747138225833 1101 86-87 0.557815384690926 1101 88-89 0.49899058833452514 1101 90-91 0.5361134644477126 1101 92-93 0.5202650214698963 1101 94-95 0.5218463275999738 1101 96-97 0.5473386341669304 1101 98-99 0.5114573743183186 1101 100-101 0.435772368036357 1101 102-103 0.29567178281500617 1101 104-105 0.45441714776644915 1101 106-107 0.4927461354391447 1101 108-109 0.4904068203339911 1101 110-111 0.5202930775563424 1101 112-113 0.605064568391704 1101 114-115 0.5818950730438708 1101 116-117 0.6169756952321883 1101 118-119 0.6424888896766205 1101 120-121 0.5825653470275611 1101 122-123 0.5564928844021004 1101 124-125 0.7248975855393951 1102 1 0.2803923921911604 1102 2 0.6280042565403541 1102 3 0.41393111054791376 1102 4 0.5362876352821928 1102 5 0.3079297737457267 1102 6 0.2693168728455575 1102 7 0.2860677381164223 1102 8 0.22923440467089762 1102 9 0.32931388508178117 1102 10-11 0.3098053242368195 1102 12-13 0.2706371845283897 1102 14-15 0.41139255206441305 1102 16-17 0.49628872075024333 1102 18-19 0.3766859998580401 1102 20-21 0.3382386939456268 1102 22-23 0.25268842438759265 1102 24-25 0.3001108528233445 1102 26-27 0.30877790963898377 1102 28-29 0.3994739303872592 1102 30-31 0.3125164416770403 1102 32-33 0.34806476268983744 1102 34-35 0.36350564045953604 1102 36-37 0.3255115566473137 1102 38-39 0.30853444312624845 1102 40-41 0.3122741872244177 1102 42-43 0.2347641784165475 1102 44-45 0.20078746451491725 1102 46-47 0.2594154258238106 1102 48-49 0.32124262491778666 1102 50-51 0.3185860693557103 1102 52-53 0.2436745642150271 1102 54-55 0.2702635612605775 1102 56-57 0.31226849990667915 1102 58-59 0.3178790521554049 1102 60-61 0.26521329875800603 1102 62-63 0.25574628893684803 1102 64-65 0.277945169346836 1102 66-67 0.2718171608175979 1102 68-69 0.29231182530462263 1102 70-71 0.17113758078954788 1102 72-73 0.15122784200309525 1102 74-75 0.15566927709753742 1102 76-77 0.1222299649717371 1102 78-79 0.10482069969020813 1102 80-81 0.11384084149474916 1102 82-83 0.1416470830894525 1102 84-85 0.10399655310515854 1102 86-87 0.11656725298703918 1102 88-89 0.11522278457156077 1102 90-91 0.10140107214949268 1102 92-93 0.09464793425343743 1102 94-95 0.11780696150185577 1102 96-97 0.07819079699443066 1102 98-99 0.09201610475615851 1102 100-101 0.12390044522996035 1102 102-103 0.12241973867926248 1102 104-105 0.15995774474200886 1102 106-107 0.1696688319399371 1102 108-109 0.16514251138375258 1102 110-111 0.24277087780251705 1102 112-113 0.19306076585961307 1102 114-115 0.18332486906989587 1102 116-117 0.1910503391569307 1102 118-119 0.28063784782678525 1102 120-121 0.29016326772388723 1102 122-123 0.32534069519865483 1102 124-125 0.3829340063236373 1103 1 0.28727790125329733 1103 2 0.5678896297938998 1103 3 0.3987524202580701 1103 4 0.5138854577282927 1103 5 0.16491614618549022 1103 6 0.14942043092332113 1103 7 0.14256737363407268 1103 8 0.12615910497944327 1103 9 0.22664976871796938 1103 10-11 0.23718607436188677 1103 12-13 0.23233265281573523 1103 14-15 0.3937095889034339 1103 16-17 0.4114230451105527 1103 18-19 0.2440985334881134 1103 20-21 0.19073670647007646 1103 22-23 0.26259731856210067 1103 24-25 0.2501013851393452 1103 26-27 0.21101739601410685 1103 28-29 0.2823783393890551 1103 30-31 0.24704244150741062 1103 32-33 0.24990447258550574 1103 34-35 0.34073468621361513 1103 36-37 0.28393188397286195 1103 38-39 0.18561931302913592 1103 40-41 0.2407666018045731 1103 42-43 0.20118221605216746 1103 44-45 0.17664849367564983 1103 46-47 0.13139724089223392 1103 48-49 0.11113871389871122 1103 50-51 0.16640009451841564 1103 52-53 0.10716616152805614 1103 54-55 0.17361565147827918 1103 56-57 0.10880317323601929 1103 58-59 -0.005256995041577284 1103 60-61 -0.014679319857108908 1103 62-63 0.013646839739998029 1103 64-65 0.02152606617740105 1103 66-67 0.10384863727708193 1103 68-69 0.13638772566727653 1103 70-71 0.1050933053096017 1103 72-73 0.13589999039840706 1103 74-75 0.18119803819036662 1103 76-77 0.1842786563781118 1103 78-79 0.16281734312448037 1103 80-81 0.1224934390890553 1103 82-83 0.07876545691844683 1103 84-85 0.08841499977693346 1103 86-87 0.035774325393951756 1103 88-89 0.04364514768401406 1103 90-91 0.04289544843913262 1103 92-93 0.04320221018429038 1103 94-95 0.1230618616154402 1103 96-97 0.10643770936192354 1103 98-99 0.05722803594360926 1103 100-101 0.05262076192700604 1103 102-103 -0.09629352455966256 1103 104-105 0.039340490662919336 1103 106-107 0.08794242157038568 1103 108-109 0.09041265909841201 1103 110-111 0.15077021862089524 1103 112-113 0.18621434202729858 1103 114-115 0.1412565471487781 1103 116-117 0.1446631161152041 1103 118-119 0.11128235219520732 1103 120-121 0.1759892053936447 1103 122-123 0.2179775612731305 1103 124-125 0.2201772381709155 1104 1 0.2965001357449637 1104 2 0.6230974988250075 1104 3 0.089477498798189 1104 4 0.41128309495554305 1104 5 0.1905807583022039 1104 6 0.180495263881987 1104 7 0.19673574302211705 1104 8 0.19081496181704694 1104 9 0.300733597150284 1104 10-11 0.2922808652300901 1104 12-13 0.2851066713966972 1104 14-15 0.3126813104292978 1104 16-17 0.36002470449480484 1104 18-19 0.34563617484757003 1104 20-21 0.27438207105127077 1104 22-23 0.1530364003633906 1104 24-25 0.18974659577034458 1104 26-27 0.29043205212618517 1104 28-29 0.38651583813636137 1104 30-31 0.39202570714833485 1104 32-33 0.2912634067880546 1104 34-35 0.23726826790917244 1104 36-37 0.31209983234877114 1104 38-39 0.3118121583484168 1104 40-41 0.3488684187661235 1104 42-43 0.3900345361729265 1104 44-45 0.4696642731673819 1104 46-47 0.3745466252392262 1104 48-49 0.3147033526947496 1104 50-51 0.28766844797408453 1104 52-53 0.34530349987699793 1104 54-55 0.2939361567035803 1104 56-57 0.357988654470077 1104 58-59 0.3661738390057607 1104 60-61 0.32002505681521853 1104 62-63 0.25907501606427985 1104 64-65 0.24262944037335643 1104 66-67 0.2697259397795406 1104 68-69 0.2660078434915647 1104 70-71 0.2566910893403218 1104 72-73 0.24772206884422587 1104 74-75 0.30455580204903043 1104 76-77 0.3261248174164635 1104 78-79 0.28236910491382616 1104 80-81 0.2650111507497428 1104 82-83 0.23895469609551867 1104 84-85 0.16507863580106275 1104 86-87 0.2389550962357312 1104 88-89 0.19218639572303076 1104 90-91 0.21995180522528557 1104 92-93 0.14699429494126193 1104 94-95 0.12601707393102757 1104 96-97 0.14949929776788906 1104 98-99 0.2277553126667975 1104 100-101 0.18597699300060455 1104 102-103 0.12972258701312 1104 104-105 0.16803378399607283 1104 106-107 0.1751512763663392 1104 108-109 0.16077511776627773 1104 110-111 0.1340028126440238 1104 112-113 0.08494503880909221 1104 114-115 0.0714741891960422 1104 116-117 0.061715128443044165 1104 118-119 0.1618662412135059 1104 120-121 0.12238951807642806 1104 122-123 0.018132579444895214 1104 124-125 0.11693536414838235 1105 1 0.19537337185029457 1105 2 0.5327500219220234 1105 3 0.2282356801552723 1105 4 0.4382956660927775 1105 5 0.21276315871007512 1105 6 0.09729685751376849 1105 7 0.13300407182430263 1105 8 0.10491442451820632 1105 9 0.13913338217818705 1105 10-11 0.21752008081845986 1105 12-13 0.1934464324307683 1105 14-15 0.29950247624557846 1105 16-17 0.2577474588182298 1105 18-19 0.20752116871771165 1105 20-21 0.18988019924349686 1105 22-23 0.16854635464896717 1105 24-25 0.1526841447993661 1105 26-27 0.17342796949595396 1105 28-29 0.23388814589335993 1105 30-31 0.22727909433857718 1105 32-33 0.14892763508947127 1105 34-35 0.14343117365915248 1105 36-37 0.17755873733135985 1105 38-39 0.1581706085520267 1105 40-41 0.12733589503837806 1105 42-43 0.05049468289532655 1105 44-45 0.15205962209627444 1105 46-47 0.17935626455652454 1105 48-49 0.310856094574504 1105 50-51 0.2793630483112608 1105 52-53 0.15508844236933328 1105 54-55 0.13312043877430568 1105 56-57 0.17038348436486928 1105 58-59 0.1803208031818997 1105 60-61 0.15191924218791542 1105 62-63 0.1843576268452125 1105 64-65 0.20995775506307268 1105 66-67 0.16721723117579046 1105 68-69 0.2111402563168383 1105 70-71 0.14419849707379484 1105 72-73 0.18632319311149814 1105 74-75 0.1260146426451243 1105 76-77 0.13240066235798054 1105 78-79 0.22558273409222096 1105 80-81 0.17100185544036606 1105 82-83 0.14867958831381856 1105 84-85 0.18438243368524 1105 86-87 0.21758636390095631 1105 88-89 0.2463663284194162 1105 90-91 0.19851563130813332 1105 92-93 0.15163836895158056 1105 94-95 0.12065492610222961 1105 96-97 0.20730511068697766 1105 98-99 0.2169225126026717 1105 100-101 0.12014812839754896 1105 102-103 0.0023061876380623403 1105 104-105 0.1562410008038384 1105 106-107 0.13955549092321107 1105 108-109 0.128691682049638 1105 110-111 0.14385329140370473 1105 112-113 0.19389131418099126 1105 114-115 0.1804799563875683 1105 116-117 0.15298944256247538 1105 118-119 0.1792236283827222 1105 120-121 0.14707422128747538 1105 122-123 0.15813627838409516 1105 124-125 0.14641006222164776 1106 1 0.20732380525225125 1106 2 0.46966860823144785 1106 3 0.3890559745430977 1106 4 0.44903692921464256 1106 5 0.19428255866134236 1106 6 0.09029402403660924 1106 7 0.11408458535118626 1106 8 -0.005655315680058948 1106 9 0.15680118165330015 1106 10-11 0.09088206278347144 1106 12-13 0.13088427923403856 1106 14-15 0.21075144851555194 1106 16-17 0.18490641989602352 1106 18-19 0.1010646459445752 1106 20-21 0.2029482657908943 1106 22-23 0.15095501408131895 1106 24-25 0.1462015101606653 1106 26-27 0.0991966597836722 1106 28-29 0.18319589024558525 1106 30-31 0.07140992675702051 1106 32-33 0.06654709363873934 1106 34-35 0.05243730816794567 1106 36-37 0.033444471548811805 1106 38-39 0.10371517037582834 1106 40-41 0.058355367630461785 1106 42-43 0.018077400399555188 1106 44-45 0.13936320472835462 1106 46-47 0.10716743985256016 1106 48-49 0.09028800133280868 1106 50-51 0.04203366120236751 1106 52-53 -0.004704591166131422 1106 54-55 0.0418380484934886 1106 56-57 0.07083065410719769 1106 58-59 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-0.9009324983215876 2216 84-85 -0.9638276592631421 2216 86-87 -0.9583807829583968 2216 88-89 -0.9117797388370441 2216 90-91 -0.9927022119695366 2216 92-93 -0.9899231900048449 2216 94-95 -1.0197764875376443 2216 96-97 -1.0160183398283422 2216 98-99 -1.0241678689716238 2216 100-101 -1.2161471302879079 2216 102-103 -1.2381851579504186 2216 104-105 -1.3063660559704857 2216 106-107 -1.3505073157177279 2216 108-109 -1.3780983469070769 2216 110-111 -1.3138961023310536 2216 112-113 -1.4347339875341305 2216 114-115 -1.48973168220407 2216 116-117 -1.5035144212621567 2216 118-119 -1.597249793187224 2216 120-121 -1.6452000288969408 2216 122-123 -1.8859263196006708 2216 124-125 -2.2359037759348723 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55.0 3 17.0 4 64.0 5 261.0 6 737.0 7 1482.0 8 2449.0 9 4573.0 10 8064.0 11 11040.0 12 13311.0 13 18735.0 14 27511.0 15 26994.0 16 13192.0 17 10747.0 18 10329.0 19 9840.0 20 9876.0 21 9486.0 22 10164.0 23 11317.0 24 13150.0 25 15643.0 26 19131.0 27 24034.0 28 30537.0 29 38737.0 30 50085.0 31 64253.0 32 84243.0 33 111994.0 34 156587.0 35 247248.0 36 432007.0 37 777265.0 38 382474.0 39 2385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.9172324893515 19.020145139328996 12.577570331504637 23.48505203981487 2 14.42175689903057 22.079199701133202 37.77129142908895 25.72775197074728 3 20.936367691242964 26.283110302391766 30.21012027897295 22.570401727392316 4 13.177424899538382 17.874375900230074 35.30007543347835 33.648123766753194 5 11.949017685546922 38.626393717474265 35.06483165957049 14.359756937408328 6 31.908443037282048 33.64033457424427 17.656810450067642 16.794411938406036 7 24.722181027517937 33.99231023800979 22.31759267959413 18.96791605487814 8 27.117712829766756 33.446011855773 21.36574972915268 18.070525585307564 9 27.77948216935967 14.597415790162854 20.66624478258064 36.956857257896836 10-11 24.35208397837673 27.05731343835536 26.525165607521917 22.065436975745992 12-13 26.493700130118313 25.209649737197232 27.13736690043685 21.159283232247606 14-15 25.41168652559535 24.337962859014027 26.42550415879948 23.824846456591146 16-17 21.81780205946548 28.20061297928157 29.450401659164804 20.531183302088145 18-19 22.487629324642068 25.787174195486294 32.063530158485776 19.661666321385862 20-21 21.51377050368074 25.37896054810318 32.2968914857744 20.81037746244168 22-23 20.69432007417311 26.19917155248438 30.125561458662343 22.98094691468017 24-25 21.265561692623102 26.893921812577055 29.451056351713973 22.389460143085874 26-27 22.16412196601003 25.018141393055217 30.12982558225098 22.687911058683774 28-29 21.63419321655646 24.421728815465293 32.05694240514473 21.887135562833514 30-31 22.829891279942732 25.533817330228448 31.004253934974756 20.632037454854064 32-33 20.13996513984627 25.796513758213624 31.91330470710188 22.15021639483822 34-35 19.538508359668086 26.34139309751207 32.51345133641671 21.60664720640313 36-37 22.461893640682305 25.461930998933212 30.560992627594462 21.515182732790016 38-39 23.04617291134359 24.632219340479704 30.71291618129242 21.60869156688429 40-41 21.395554101476012 25.898867383983227 30.563179724797834 22.142398789742927 42-43 22.925475572420854 25.507006181469777 30.085656054281916 21.48186219182745 44-45 23.978355610548913 24.32993826077342 29.844676468113224 21.84702966056444 46-47 21.97729297585638 25.784815720050098 31.728532307528596 20.509358996564927 48-49 23.073814243455796 25.68951444462432 30.695199313815724 20.541471998104164 50-51 21.1558454153313 26.270850337941226 30.403521939670576 22.1697823070569 52-53 20.718999161137457 27.16918419768628 28.224252901019124 23.887563740157137 54-55 21.572465384184326 27.35597545223295 27.431729683243546 23.639829480339184 56-57 22.47602562549599 25.741212226185716 27.992178918097505 23.79058323022079 58-59 22.33434728909286 24.88238754694781 29.708752811774414 23.07451235218491 60-61 23.13666289687953 26.10077595728631 28.974436767764132 21.78812437807003 62-63 20.601056521524203 26.326227341183095 29.94839278389535 23.124323353397354 64-65 19.785325905456457 26.667451567291835 30.74189712745239 22.805325399799322 66-67 22.49711982979732 26.271076751583106 28.700268715257298 22.53153470336228 68-69 23.15653069395404 25.250875749098967 28.81060763006426 22.781985926882733 70-71 21.506880899254607 26.382585470206415 28.847739467331717 23.262794163207253 72-73 22.961796094138265 26.0939269446196 28.386232993977018 22.558043967265114 74-75 23.701659272374478 24.908500586977368 28.440232647564144 22.949607493084006 76-77 21.757596272023918 26.052625322780948 30.67131267459794 21.5184657305972 78-79 22.63419442214899 25.775532760733235 30.046014799150345 21.544258017967433 80-81 20.77356484882927 26.458754792893778 29.78963908533417 22.97804127294278 82-83 20.34192610839855 27.03409072470101 27.850802466550213 24.773180700350224 84-85 21.10537404099672 27.088241320716055 27.184957681403553 24.621426956883674 86-87 21.868935180415825 25.300309394241623 27.943971680181672 24.88678374516088 88-89 21.810935552488907 24.718313127802578 29.334208044703107 24.13654327500541 90-91 22.441214528057746 25.997267187342572 28.392082013058783 23.1694362715409 92-93 20.22762495485878 25.964173060021878 29.345075899513095 24.463126085606245 94-95 19.41767814656254 26.370615793614704 30.073245421217777 24.13846063860498 96-97 22.000613974659487 25.841917940607477 28.25711764369583 23.900350441037205 98-99 22.51688305366142 24.941201066220668 28.28307490734156 24.25884097277635 100-101 20.97969560195274 25.912758295512823 28.36887461476662 24.738671487767814 102-103 22.266355638350525 25.382638982052097 28.163214014614407 24.187791364982974 104-105 22.865626045031366 24.24008527078532 28.417398833414737 24.476889850768575 106-107 21.010842165292917 25.518811970124883 30.312470396061542 23.157875468520658 108-109 21.887175008364753 25.471516342358335 29.39739246173963 23.243916187537284 110-111 20.207148287882752 25.905877473180382 29.41338870270771 24.473585536229155 112-113 19.691948458176046 26.40759887894797 27.633683492259742 26.26676917061624 114-115 20.120866005666738 26.704678975743935 27.071086025436713 26.103368993152614 116-117 20.891164478751946 25.26616341702393 27.54401829984974 26.298653804374396 118-119 20.617460182061528 24.838739142020522 28.740931081883904 25.802869594034046 120-121 21.295175431230426 25.8681266798168 27.991043367319502 24.845654521633275 122-123 19.451651632046694 26.032103751802637 28.522820992705682 25.99342362344499 124-125 18.663482739661514 26.566740013215206 29.149366202046284 25.620411045076995 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3275.0 1 2322.5 2 1995.5 3 2540.0 4 1629.5 5 1206.5 6 2649.5 7 5537.5 8 8378.5 9 9095.5 10 6886.5 11 4119.5 12 3348.5 13 3485.0 14 3806.0 15 4178.5 16 4506.5 17 5039.0 18 5603.0 19 5783.0 20 6031.0 21 6769.5 22 7755.0 23 8858.0 24 10281.0 25 12111.0 26 14797.5 27 18048.5 28 20695.0 29 23667.0 30 27665.5 31 31684.5 32 35671.5 33 40149.0 34 45364.5 35 49140.0 36 53329.5 37 57596.5 38 58707.0 39 59509.0 40 60912.0 41 64564.0 42 68422.5 43 71514.0 44 75242.0 45 78419.0 46 82366.0 47 89965.0 48 99553.5 49 131914.0 50 250279.0 51 245695.5 52 114249.5 53 72591.5 54 64817.0 55 59042.0 56 51414.5 57 44822.5 58 41827.0 59 38515.5 60 33970.5 61 29287.5 62 25098.5 63 20126.0 64 15440.5 65 12004.0 66 8437.5 67 6455.5 68 4841.5 69 3074.0 70 2503.5 71 2267.0 72 2043.0 73 1959.5 74 1588.0 75 1271.0 76 840.0 77 422.5 78 263.0 79 159.5 80 107.0 81 68.0 82 32.0 83 12.0 84 8.0 85 13.5 86 11.5 87 5.5 88 2.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1460367990092139 2 2.6414924885387526E-4 3 4.1509167677037545E-4 4 0.0 5 0.0 6 0.003094319772288253 7 0.0 8 0.0 9 0.0 10-11 5.660341046868756E-5 12-13 0.00456600844447413 14-15 0.0012641428338006889 16-17 3.396204628121254E-4 18-19 0.0 20-21 1.8867803489562522E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.2641364187475025E-4 30-31 0.0 32-33 0.0 34-35 5.660341046868756E-5 36-37 0.0 38-39 0.0 40-41 5.660341046868756E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 8.301833535407509E-4 96-97 0.0026603602920283152 98-99 0.0036414860734855664 100-101 0.0 102-103 0.003679221680464691 104-105 0.021697974012996897 106-107 0.017131965568522766 108-109 0.019113084934926833 110-111 0.015754615913784704 112-113 0.013698025333422388 114-115 0.03964125513157085 116-117 0.03899974981292573 118-119 0.021999858868829898 120-121 0.013603686315974577 122-123 0.00292450954088219 124-125 0.0015094242791650018 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2650017.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.87082527535617 #Duplication Level Percentage of deduplicated Percentage of total 1 81.14105440742831 40.46571347011026 2 11.5963834814592 11.566424288597563 3 3.459544417839898 5.17591005583282 4 1.3957389795652082 2.784266191196017 5 0.699971656171528 1.7454082081315978 6 0.4011407692775919 1.2003132729278847 7 0.25860595886178794 0.902782481269352 8 0.15870562395811955 0.6331824354105408 9 0.11918096612443502 0.5349287823965857 >10 0.6556383664994283 6.13319971396016 >50 0.06598287001608386 2.2696900627344387 >100 0.03993284241737444 3.9614556836905774 >500 0.0030615112912573744 1.0714159687883047 >1k 0.0023959653583753363 2.641763933946677 >5k 0.0011979826791876681 4.008383546675601 >10k+ 0.0014642010523404833 14.90516190433167 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 58746 2.2168159675956796 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 34053 1.2850106244601451 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 17399 0.6565618258297966 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 16518 0.6233167560811874 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16510 0.6230148712253544 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 16095 0.6073545943290175 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15714 0.5929773280699708 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 14166 0.5345626084662853 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 14083 0.5314305530870179 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 10775 0.4066011652000723 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10475 0.39528048310633485 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 8431 0.31814890244100325 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8281 0.31248856139413445 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7416 0.2798472613571913 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6904 0.26052663058387926 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 6766 0.25531911682076 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 6364 0.24014940281515176 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5615 0.2118854331877871 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5325 0.20094210716384084 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5281 0.19928174045675934 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 4857 0.18328184309761034 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4425 0.1669800608826283 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3328 0.12558410002652812 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3288 0.12407467574736314 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 2943 0.11105589133956499 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2827 0.1066785609299865 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2660 0.10037671456447261 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.773560697912504E-5 7 0.0 0.0 0.0 0.0 3.773560697912504E-5 8 0.0 0.0 0.0 0.0 3.773560697912504E-5 9 0.0 0.0 0.0 3.773560697912504E-5 3.773560697912504E-5 10-11 0.0 0.0 0.0 1.3207462442693763E-4 3.773560697912504E-5 12-13 0.0 0.0 0.0 4.5282728374950045E-4 3.773560697912504E-5 14-15 0.0 0.0 0.0 0.0011509360128633137 9.43390174478126E-5 16-17 0.0 0.0 0.0 0.002132061794320565 1.1320682093737511E-4 18-19 0.0 0.0 0.0 0.0027169637024970026 1.1320682093737511E-4 20-21 0.0 0.0 0.0 0.003490543645569066 1.1320682093737511E-4 22-23 0.0 0.0 0.0 0.005377323994525318 1.1320682093737511E-4 24-25 0.0 0.0 0.0 0.00809428769702232 1.3207462442693763E-4 26-27 0.0 0.0 0.0 0.010622573364623699 1.6981023140606266E-4 28-29 0.0 0.0 0.0 0.016113104180086393 1.886780348956252E-4 30-31 0.0 0.0 0.0 0.03088659431241384 1.886780348956252E-4 32-33 0.0 0.0 0.0 0.05832038058623775 2.2641364187475023E-4 34-35 0.0 0.0 0.0 0.10045218577843085 2.6414924885387526E-4 36-37 0.0 0.0 0.0 0.16828193932340813 2.830170523434378E-4 38-39 0.0 0.0 0.0 0.2592247521430995 3.018848558330003E-4 40-41 0.0 0.0 0.0 0.37052592492802877 3.2075265932256285E-4 42-43 0.0 0.0 0.0 0.46814794018302525 3.396204628121254E-4 44-45 0.0 0.0 0.0 0.58697736656029 3.396204628121254E-4 46-47 0.0 0.0 0.0 0.7294858863169558 3.396204628121254E-4 48-49 0.0 0.0 0.0 0.8692208389606557 3.396204628121254E-4 50-51 0.0 0.0 0.0 1.0084274931066481 3.396204628121254E-4 52-53 0.0 0.0 0.0 1.1582378528137744 3.962238732808129E-4 54-55 0.0 0.0 0.0 1.3264065853162452 4.5282728374950045E-4 56-57 0.0 0.0 0.0 1.534292044164245 4.5282728374950045E-4 58-59 0.0 0.0 0.0 1.7621962425146707 4.5282728374950045E-4 60-61 0.0 0.0 0.0 1.987440080573068 4.5282728374950045E-4 62-63 0.0 0.0 0.0 2.200250036131844 4.5282728374950045E-4 64-65 0.0 0.0 0.0 2.443606965540221 4.5282728374950045E-4 66-67 0.0 0.0 0.0 2.730982480489748 4.5282728374950045E-4 68-69 0.0 0.0 0.0 3.0685274849180213 4.7169508723906304E-4 70-71 0.0 0.0 0.0 3.4908643982283887 4.905628907286256E-4 72-73 0.0 0.0 0.0 3.862012960671573 4.905628907286256E-4 74-75 0.0 0.0 0.0 4.221293674719822 4.905628907286256E-4 76-77 0.0 0.0 0.0 4.637574023110041 4.905628907286256E-4 78-79 0.0 0.0 0.0 5.071948595046749 4.905628907286256E-4 80-81 0.0 0.0 0.0 5.541926712168261 4.905628907286256E-4 82-83 0.0 0.0 0.0 6.067074286693256 4.905628907286256E-4 84-85 0.0 0.0 0.0 6.575561590736966 4.905628907286256E-4 86-87 0.0 0.0 0.0 7.10453932937034 4.905628907286256E-4 88-89 0.0 0.0 0.0 7.697705335475206 5.282984977077505E-4 90-91 0.0 0.0 0.0 8.302776925581988 5.282984977077505E-4 92-93 0.0 0.0 0.0 8.917131475005633 5.282984977077505E-4 94-95 0.0 0.0 0.0 9.566089576029135 5.660341046868756E-4 96-97 0.0 0.0 0.0 10.217255210060916 5.660341046868756E-4 98-99 0.0 0.0 0.0 10.950005226381567 5.660341046868756E-4 100-101 0.0 0.0 0.0 11.872697420431642 6.037697116660006E-4 102-103 0.0 0.0 0.0 12.702560021313072 6.415053186451257E-4 104-105 0.0 0.0 0.0 13.48232860392971 6.792409256242508E-4 106-107 0.0 3.773560697912504E-5 0.0 14.288700789466635 6.981087291138132E-4 108-109 0.0 3.773560697912504E-5 0.0 15.076563659780295 7.358443360929382E-4 110-111 0.0 3.773560697912504E-5 0.0 15.9182186378427 7.924477465616258E-4 112-113 0.0 3.773560697912504E-5 0.0 16.858495624745046 7.924477465616258E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTATCA 1575 0.0 29.133501 1 AGTACTC 1720 0.0 25.59746 5 CCCTATA 400 0.0 23.79917 2 GAGTACT 18150 0.0 23.684883 12-13 GTATAGG 925 0.0 23.192272 1 GTACTTT 19700 0.0 21.865004 14-15 TTATCAA 2080 0.0 21.739626 2 CGCTAGG 140 0.0031573747 21.249258 2 CATGGGG 6430 0.0 20.634195 4 AGTACTT 18600 0.0 20.488785 12-13 ATGGGAG 3500 0.0 20.228912 5 ACTTTTT 22515 0.0 19.263393 16-17 TAACACG 155 0.0056918557 19.192518 4 TATGTCG 155 0.0056918557 19.192518 5 AAGTACT 2315 0.0 18.504404 4 CCTATAC 625 0.0 18.08737 3 AAAGTAC 2315 0.0 17.990732 3 TACTTTT 21915 0.0 17.456081 14-15 TATAGGG 485 2.7102942E-10 17.17466 2 ATGGGAT 3445 0.0 17.097775 5 >>END_MODULE