##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364162_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1107325 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.313457205427493 31.0 31.0 34.0 26.0 34.0 2 30.343982119070734 31.0 31.0 34.0 26.0 34.0 3 30.530049443478653 33.0 31.0 34.0 26.0 34.0 4 33.752043889553654 37.0 35.0 37.0 28.0 37.0 5 33.87650418802068 37.0 35.0 37.0 30.0 37.0 6 33.73946944212404 37.0 35.0 37.0 28.0 37.0 7 33.65088117761272 37.0 35.0 37.0 28.0 37.0 8 33.5254997403653 37.0 35.0 37.0 27.0 37.0 9 34.832137809586165 39.0 35.0 39.0 26.0 39.0 10-11 34.822486171629826 39.0 35.0 39.0 26.5 39.0 12-13 34.704967376334864 39.0 35.0 39.0 25.5 39.0 14-15 35.866066421330686 40.0 36.0 41.0 25.0 41.0 16-17 35.92146975820107 40.0 36.0 41.0 26.0 41.0 18-19 35.870879371458244 40.0 36.0 41.0 25.5 41.0 20-21 35.91291355293161 40.0 36.0 41.0 27.0 41.0 22-23 35.92792089043415 40.0 36.0 41.0 27.0 41.0 24-25 35.94229968618066 40.0 36.0 41.0 27.0 41.0 26-27 35.758242611699366 40.0 36.0 41.0 25.5 41.0 28-29 35.64079922335358 40.0 36.0 41.0 25.0 41.0 30-31 35.709614611789675 40.0 36.0 41.0 26.0 41.0 32-33 35.40442191768451 39.5 35.0 41.0 25.5 41.0 34-35 35.44938206940148 40.0 35.0 41.0 25.5 41.0 36-37 35.463761316686615 40.0 35.0 41.0 25.5 41.0 38-39 35.32568125888967 40.0 35.0 41.0 25.0 41.0 40-41 34.95049059670828 39.0 34.0 41.0 24.5 41.0 42-43 34.7160693563317 39.0 34.0 41.0 24.0 41.0 44-45 34.45328742690719 39.0 33.0 41.0 23.0 41.0 46-47 34.166846228523696 38.0 33.0 40.0 23.0 41.0 48-49 33.89457656966112 38.0 33.0 40.0 20.0 41.0 50-51 33.814730544329805 38.0 33.0 40.0 19.0 41.0 52-53 33.65567471157971 38.0 33.0 40.0 18.0 41.0 54-55 33.35142799087892 38.0 32.5 40.0 14.5 41.0 56-57 32.982633373219244 38.0 32.0 40.0 10.0 41.0 58-59 32.577814101551034 37.0 31.0 40.0 8.0 41.0 60-61 32.295830040864246 37.0 31.0 40.0 6.0 41.0 62-63 31.7223461946583 36.0 30.0 39.0 2.0 41.0 64-65 31.484141060664214 36.0 30.0 39.0 2.0 41.0 66-67 31.02854491680401 35.0 30.0 39.0 2.0 41.0 68-69 30.60247578624162 35.0 29.5 38.0 2.0 40.0 70-71 29.94386155825977 35.0 28.5 37.5 2.0 40.0 72-73 29.671874111033347 34.0 28.5 37.0 2.0 39.0 74-75 29.38457453773734 34.0 28.5 36.5 2.0 39.0 76-77 28.846540988418035 34.0 28.0 36.0 2.0 39.0 78-79 28.431112816923665 34.0 27.0 35.5 2.0 37.0 80-81 28.168213487458516 34.0 27.0 35.0 2.0 37.0 82-83 27.921182128101506 34.0 26.5 35.0 2.0 36.5 84-85 27.608529112952382 34.0 26.0 35.0 2.0 36.0 86-87 27.208441965999143 33.5 26.0 35.0 2.0 36.0 88-89 26.858435418689183 33.0 25.0 35.0 2.0 36.0 90-91 26.687833291942294 33.0 25.0 35.0 2.0 35.0 92-93 26.286863386991172 33.0 24.0 35.0 2.0 35.0 94-95 26.197648838416903 33.0 24.0 35.0 2.0 35.0 96-97 26.09324272458402 33.0 23.5 35.0 2.0 35.0 98-99 25.837614069943335 33.0 22.0 35.0 2.0 35.0 100-101 25.22004831463211 32.5 18.0 35.0 2.0 35.0 102-103 23.860012643081298 30.0 15.0 34.0 2.0 35.0 104-105 24.660079470796738 32.0 17.0 35.0 2.0 35.0 106-107 24.70733659946267 32.0 16.0 35.0 2.0 35.0 108-109 24.624007405233336 32.0 13.0 35.0 2.0 35.0 110-111 24.65862687106315 32.0 10.0 35.0 2.0 35.0 112-113 24.542351161583092 32.0 7.5 35.0 2.0 35.0 114-115 24.34184859910144 32.0 3.5 35.0 2.0 35.0 116-117 23.935976339376424 32.0 2.0 35.0 2.0 35.0 118-119 23.650960196870837 32.0 2.0 35.0 2.0 35.0 120-121 23.415291355293164 32.0 2.0 35.0 2.0 35.0 122-123 22.87409748718759 32.0 2.0 35.0 2.0 35.0 124-125 21.797992459305082 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4049099832176388 1101 2 0.45127294126942985 1101 3 0.42584138892454604 1101 4 0.48686111183774017 1101 5 0.4546249539353866 1101 6 0.38751153583498876 1101 7 0.4510362872398943 1101 8 0.429026275397959 1101 9 0.629720248506267 1101 10-11 0.6777544873736474 1101 12-13 0.6869045887632765 1101 14-15 0.6461394555786057 1101 16-17 0.6642310032157894 1101 18-19 0.7967107692859585 1101 20-21 0.7958463182062516 1101 22-23 0.7111034655795976 1101 24-25 0.7576518520525184 1101 26-27 0.8322182937508487 1101 28-29 0.8982448987130951 1101 30-31 0.8563103113215504 1101 32-33 0.9161156918749782 1101 34-35 0.8810672840127651 1101 36-37 0.9745508086597638 1101 38-39 1.0179235796115123 1101 40-41 0.982094994793087 1101 42-43 0.9928178227412161 1101 44-45 0.9012798192998588 1101 46-47 0.9508588765901678 1101 48-49 0.9718139849174392 1101 50-51 0.9331560810279953 1101 52-53 0.9371077175997655 1101 54-55 1.1087346848333084 1101 56-57 0.9463697094180716 1101 58-59 0.9784327130440431 1101 60-61 0.9025352170905023 1101 62-63 1.2112611344179243 1101 64-65 1.1761291029526006 1101 66-67 1.173742814430092 1101 68-69 1.232419763322472 1101 70-71 1.108654371205116 1101 72-73 1.213155774631634 1101 74-75 1.1250573569374076 1101 76-77 1.0233147709164037 1101 78-79 1.0997832356921897 1101 80-81 1.0653194212803179 1101 82-83 1.0317245968373072 1101 84-85 1.03986471129895 1101 86-87 0.9378582934731483 1101 88-89 0.9518867518388632 1101 90-91 1.0321114873905373 1101 92-93 1.129758409470508 1101 94-95 1.1024168954298474 1101 96-97 1.1174330858829506 1101 98-99 1.177551202561716 1101 100-101 0.9765701881696494 1101 102-103 0.9159960276819561 1101 104-105 0.793546354026784 1101 106-107 0.6560189846177487 1101 108-109 0.7562977242375233 1101 110-111 0.7905085546304882 1101 112-113 0.544037180402043 1101 114-115 0.6693223428686004 1101 116-117 0.8537359846652777 1101 118-119 0.8695291864332049 1101 120-121 1.0006565899077238 1101 122-123 0.9282209232831455 1101 124-125 1.0081523966897308 1102 1 0.2603652808668464 1102 2 0.3642344445245804 1102 3 0.467137446863493 1102 4 0.4193560924753612 1102 5 0.37905828242766404 1102 6 0.4319953000038552 1102 7 0.4652623409179668 1102 8 0.46119121425098797 1102 9 0.4584399812799589 1102 10-11 0.39227333257107944 1102 12-13 0.4098566648927715 1102 14-15 0.40886505896453684 1102 16-17 0.5138866415127552 1102 18-19 0.5159285802342097 1102 20-21 0.4502761988317232 1102 22-23 0.5078197512255684 1102 24-25 0.5037613611805014 1102 26-27 0.5977136465215764 1102 28-29 0.6474635854860438 1102 30-31 0.6297941460737562 1102 32-33 0.7378413917124931 1102 34-35 0.6715107062921959 1102 36-37 0.7346986419544734 1102 38-39 0.6629422250691732 1102 40-41 0.6933572133789738 1102 42-43 0.9173738405991685 1102 44-45 0.9636442651042358 1102 46-47 0.8774802886191182 1102 48-49 0.9236323558761299 1102 50-51 0.8187973971892575 1102 52-53 0.7660325485600552 1102 54-55 0.784051506099658 1102 56-57 0.804196385559905 1102 58-59 0.8571871646909486 1102 60-61 0.8474238561661025 1102 62-63 0.8420030983568552 1102 64-65 0.7313311874892676 1102 66-67 0.7819882788961827 1102 68-69 0.7453002989871997 1102 70-71 0.826178551542224 1102 72-73 0.7814255700249433 1102 74-75 0.8156854045394013 1102 76-77 0.8682522270558799 1102 78-79 0.8820968245372107 1102 80-81 0.8687054629998805 1102 82-83 0.8372604740533376 1102 84-85 0.8009487900995182 1102 86-87 0.9317737139373641 1102 88-89 0.8755717491097634 1102 90-91 0.8734364438487461 1102 92-93 0.9072067367377379 1102 94-95 0.9016678990251599 1102 96-97 0.9827639536034916 1102 98-99 0.8848326685961609 1102 100-101 0.8546167739130688 1102 102-103 0.62446336773586 1102 104-105 0.8576515475932815 1102 106-107 0.7892568641464024 1102 108-109 0.7125072288939869 1102 110-111 0.7058983465057196 1102 112-113 0.7119481094007476 1102 114-115 0.518959503851459 1102 116-117 0.6158967365589234 1102 118-119 0.532726922149223 1102 120-121 0.36754491043442883 1102 122-123 0.4641907106573022 1102 124-125 0.4560596745711649 1103 1 0.2947648777627059 1103 2 0.3136579333082672 1103 3 0.3134543631463451 1103 4 0.30831580493168076 1103 5 0.14472596792085568 1103 6 0.22739523552490226 1103 7 0.32501729137467095 1103 8 0.26984142771764397 1103 9 0.35901918526732857 1103 10-11 0.3578027276714124 1103 12-13 0.3173125790664102 1103 14-15 0.18701539821680058 1103 16-17 0.19766789702279652 1103 18-19 0.23674041750821573 1103 20-21 0.29805440450188314 1103 22-23 0.3219137886927115 1103 24-25 0.3865289912690031 1103 26-27 0.5691278529914641 1103 28-29 0.48062483968288916 1103 30-31 0.3524044224258347 1103 32-33 0.5517698053162192 1103 34-35 0.3535886918928739 1103 36-37 0.4159071944904369 1103 38-39 0.38958778483725354 1103 40-41 0.553760642124935 1103 42-43 0.5186731210759916 1103 44-45 0.5083033197128941 1103 46-47 0.5172981835213761 1103 48-49 0.5867038043139914 1103 50-51 0.47749899462957757 1103 52-53 0.4562021267393064 1103 54-55 0.5411831073541222 1103 56-57 0.45571381737931205 1103 58-59 0.5813983487650844 1103 60-61 0.5629536201391829 1103 62-63 0.4587544582062435 1103 64-65 0.38948777544586477 1103 66-67 0.34694160542750296 1103 68-69 0.198191744901969 1103 70-71 0.39385895429224504 1103 72-73 0.2593011359679558 1103 74-75 0.14060917698623143 1103 76-77 0.11510334485096507 1103 78-79 0.19310210605720357 1103 80-81 0.22085259748571673 1103 82-83 0.1509762325184134 1103 84-85 0.17155007480952378 1103 86-87 0.18344396973586186 1103 88-89 0.09753188724523199 1103 90-91 0.21679961160985783 1103 92-93 0.14219343149775554 1103 94-95 0.20404192713768765 1103 96-97 0.21769125985123594 1103 98-99 0.20795486654655804 1103 100-101 0.4088193042325621 1103 102-103 0.304671168509163 1103 104-105 0.31492298850613665 1103 106-107 0.0964546411716789 1103 108-109 0.17300962619797033 1103 110-111 0.06495280761583544 1103 112-113 0.015049923443140045 1103 114-115 -0.056671397651403055 1103 116-117 -0.09115522506945695 1103 118-119 -0.12654310819424808 1103 120-121 -0.19963271876140354 1103 122-123 -0.14153504278372608 1103 124-125 0.01826932561017003 1104 1 0.2876233058171991 1104 2 0.3535741003610724 1104 3 0.2747095767900447 1104 4 0.28003014501562973 1104 5 0.3459894597163782 1104 6 0.3152187998245921 1104 7 0.18734636938255278 1104 8 0.10118482070846113 1104 9 0.38234017541721954 1104 10-11 0.40267449406463385 1104 12-13 0.37087714531864435 1104 14-15 0.557867344433987 1104 16-17 0.4429077746127703 1104 18-19 0.3366096923110149 1104 20-21 0.4071059119325966 1104 22-23 0.3338360303392136 1104 24-25 0.4493513153268154 1104 26-27 0.4241528318333039 1104 28-29 0.5355199818590322 1104 30-31 0.4853694594516895 1104 32-33 0.5555482855275145 1104 34-35 0.4961311729542004 1104 36-37 0.5498209240051608 1104 38-39 0.4440827176161477 1104 40-41 0.49033726352832474 1104 42-43 0.4438404426644169 1104 44-45 0.3896362354364129 1104 46-47 0.31979688219294644 1104 48-49 0.3660416336167245 1104 50-51 0.3893866575918068 1104 52-53 0.4120500708625272 1104 54-55 0.3516515633751425 1104 56-57 0.38060476864853854 1104 58-59 0.4253222371566423 1104 60-61 0.4851886026610259 1104 62-63 0.5004652737818986 1104 64-65 0.47292440056628493 1104 66-67 0.3483984268689504 1104 68-69 0.2722691715370331 1104 70-71 0.2557408047775702 1104 72-73 0.2911751673760641 1104 74-75 0.17610415937479473 1104 76-77 0.12200094085494229 1104 78-79 0.21389948163132644 1104 80-81 -0.09149861976541729 1104 82-83 0.160695471372712 1104 84-85 -0.016809718251487027 1104 86-87 0.02043638685993443 1104 88-89 0.08433367734002672 1104 90-91 0.18456054184310489 1104 92-93 0.2489060648607797 1104 94-95 0.19371293354030072 1104 96-97 0.0971101306313571 1104 98-99 -0.04529845452503878 1104 100-101 -0.09126732758885936 1104 102-103 -0.2894718909861673 1104 104-105 -0.20754482629131488 1104 106-107 -0.17424999392694218 1104 108-109 0.07717613552052072 1104 110-111 -0.04759229461341974 1104 112-113 0.07753830774486303 1104 114-115 0.033322219730646196 1104 116-117 -0.061339810084522384 1104 118-119 -0.012642254981869883 1104 120-121 0.21553621699258585 1104 122-123 0.18987776673066747 1104 124-125 0.03673152933119184 1105 1 0.21988620427552164 1105 2 0.29407344868830165 1105 3 0.22071507209387775 1105 4 0.1483716432340998 1105 5 0.05742637106936854 1105 6 -3.317823436432832E-4 1105 7 0.17397569227176746 1105 8 0.14002169866457592 1105 9 0.3375731836731859 1105 10-11 0.23468496420153429 1105 12-13 0.20331754599439478 1105 14-15 0.28781946112353296 1105 16-17 0.32874196997580896 1105 18-19 0.23787693019434641 1105 20-21 0.171050733356914 1105 22-23 0.2118810553977255 1105 24-25 0.23277367084737932 1105 26-27 0.3492180533261475 1105 28-29 0.26581853690207424 1105 30-31 0.2349281436148516 1105 32-33 0.06238434374216695 1105 34-35 0.1980147349693695 1105 36-37 0.2513049162761689 1105 38-39 0.2214920093204924 1105 40-41 0.21012014047385463 1105 42-43 0.2116763252201963 1105 44-45 0.3332508181598257 1105 46-47 0.5228124681237531 1105 48-49 0.5922512402058828 1105 50-51 0.48119932715142255 1105 52-53 0.22852015138369097 1105 54-55 0.2758628875178317 1105 56-57 0.38544785755578914 1105 58-59 0.17538838031646975 1105 60-61 0.29569332918616453 1105 62-63 0.34334545056847077 1105 64-65 0.34360639075974575 1105 66-67 0.26798244060938003 1105 68-69 0.20739194842206032 1105 70-71 0.13835187649193514 1105 72-73 0.200913066972916 1105 74-75 0.26606698908760507 1105 76-77 0.25564211095996825 1105 78-79 0.2823500618477972 1105 80-81 0.20901794746964697 1105 82-83 0.25946130506929066 1105 84-85 0.4706529000200703 1105 86-87 0.5766607834933559 1105 88-89 0.3322686011452767 1105 90-91 0.4752423802325936 1105 92-93 0.4221913847659664 1105 94-95 0.41436163529103354 1105 96-97 0.5050513217720791 1105 98-99 0.49603885173513973 1105 100-101 0.48867535556805564 1105 102-103 0.32122519285950446 1105 104-105 0.5479938501081527 1105 106-107 0.5625343858779246 1105 108-109 0.5749088347469673 1105 110-111 0.6346397008931852 1105 112-113 0.51306444947339 1105 114-115 0.5433071260784352 1105 116-117 0.36678263500039066 1105 118-119 0.34778049739765393 1105 120-121 0.32952636274608693 1105 122-123 0.25598134908757686 1105 124-125 0.2926632695902569 1106 1 0.19444686470199457 1106 2 0.21092737025074726 1106 3 0.1755062399688576 1106 4 0.1786747053859088 1106 5 0.1048146469860356 1106 6 0.2119707767248542 1106 7 0.18988973088867311 1106 8 0.150051484279615 1106 9 0.20489301783786829 1106 10-11 0.21299177355328425 1106 12-13 0.3393120709345041 1106 14-15 0.18947280449050652 1106 16-17 0.23852837677682714 1106 18-19 0.20878795942012118 1106 20-21 0.201088590726485 1106 22-23 0.2801504417972325 1106 24-25 0.3362280594785432 1106 26-27 0.3265393659111524 1106 28-29 0.36002263878274476 1106 30-31 0.2868173685932831 1106 32-33 0.040319971499755525 1106 34-35 0.15750198937468696 1106 36-37 0.17392677909978005 1106 38-39 0.082496150999404 1106 40-41 0.11402702381668206 1106 42-43 0.08247938071560412 1106 44-45 0.10368857771543105 1106 46-47 0.30278936693044045 1106 48-49 0.07477364948447729 1106 50-51 0.16862010729490606 1106 52-53 0.07768063986497253 1106 54-55 0.1895587000849659 1106 56-57 0.2579507460807946 1106 58-59 0.4263696730443911 1106 60-61 0.2753319430024632 1106 62-63 0.1341200727479972 1106 64-65 0.22307116757082923 1106 66-67 0.2819846925728413 1106 68-69 0.3116743575324925 1106 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38-39 0.8893779063366338 2204 40-41 0.6884956983738348 2204 42-43 0.7560281697407731 2204 44-45 0.8685505416083572 2204 46-47 0.7775139145086527 2204 48-49 0.836951859147625 2204 50-51 0.8779884554043988 2204 52-53 0.9050547909359068 2204 54-55 0.9594466650668778 2204 56-57 0.9939960942815702 2204 58-59 1.1266846128108696 2204 60-61 1.1834479908653535 2204 62-63 1.1292768127324848 2204 64-65 1.2119614337760893 2204 66-67 1.152336560598627 2204 68-69 1.0268101488901387 2204 70-71 1.0138327386591648 2204 72-73 0.8158026568107744 2204 74-75 0.9153892703483706 2204 76-77 0.9728396965379211 2204 78-79 1.0410654991550707 2204 80-81 1.0297716483161352 2204 82-83 0.9505988896058035 2204 84-85 1.0440904412584828 2204 86-87 1.0538495667881378 2204 88-89 1.1311086114455193 2204 90-91 1.1997669392446255 2204 92-93 1.0625828081750477 2204 94-95 1.2120573131132026 2204 96-97 1.211539938365597 2204 98-99 1.1994771633516557 2204 100-101 1.1116956443314514 2204 102-103 1.0899157783397335 2204 104-105 1.0502548741831816 2204 106-107 1.237326223293902 2204 108-109 1.1735468347883398 2204 110-111 1.2290142287691026 2204 112-113 1.2768973730083708 2204 114-115 1.3083854942178803 2204 116-117 1.4002746448241368 2204 118-119 1.381515568646929 2204 120-121 1.4058431921551282 2204 122-123 1.3919678860168645 2204 124-125 1.556568772729193 2205 1 0.1616312115854015 2205 2 0.2130508412014933 2205 3 0.2860307755512075 2205 4 0.15777108429407605 2205 5 0.2286678153520043 2205 6 0.29934307885483236 2205 7 0.2402119521415429 2205 8 0.235893843288153 2205 9 0.3871525425293356 2205 10-11 0.22320134608452946 2205 12-13 0.18717980433038406 2205 14-15 0.2767964802441014 2205 16-17 0.20515798966796694 2205 18-19 0.2860366790447273 2205 20-21 0.3018411911649821 2205 22-23 0.4091551456840534 2205 24-25 0.48034877302770695 2205 26-27 0.5533801602945161 2205 28-29 0.5409762234763136 2205 30-31 0.44754077175313967 2205 32-33 0.5622173941587931 2205 34-35 0.4811343010297122 2205 36-37 0.43031220892120103 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104-105 1.354533225770023 2206 106-107 1.274016238108377 2206 108-109 1.4052394548576181 2206 110-111 1.1027321173014677 2206 112-113 1.1928341860829512 2206 114-115 1.2234543668341793 2206 116-117 1.3405888665051613 2206 118-119 1.2501956756093051 2206 120-121 1.1144493844193484 2206 122-123 1.0004018519457212 2206 124-125 1.0694031466115632 2207 1 0.060691140397800325 2207 2 0.005857757895739724 2207 3 -0.008756155194753035 2207 4 0.03879169153709938 2207 5 -0.03645084032877577 2207 6 0.1695287704952193 2207 7 0.17791844554246694 2207 8 0.25207653877824754 2207 9 0.14703664493264768 2207 10-11 0.09581705497021176 2207 12-13 0.12980802620808163 2207 14-15 0.12586468330876954 2207 16-17 0.06629498805956757 2207 18-19 0.13848135768438397 2207 20-21 0.0966782029115123 2207 22-23 0.14482544721522572 2207 24-25 0.2994772474878502 2207 26-27 0.4592823150840033 2207 28-29 0.3914372590062385 2207 30-31 0.1830034911659908 2207 32-33 0.39146876303946954 2207 34-35 0.17348453706364353 2207 36-37 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0.3153100658103156 2208 36-37 0.2626534180598057 2208 38-39 0.38022328964562035 2208 40-41 0.28971060944533633 2208 42-43 0.2750277305073041 2208 44-45 0.25718694964827904 2208 46-47 0.08529988841659275 2208 48-49 0.24298610922704 2208 50-51 0.16245417167694853 2208 52-53 0.1689886946214827 2208 54-55 0.2542204381257278 2208 56-57 0.34453008494550375 2208 58-59 0.312497552903622 2208 60-61 0.2659377716321387 2208 62-63 0.2673393951792207 2208 64-65 0.23440810888008912 2208 66-67 0.19131581676725062 2208 68-69 0.2017444628646352 2208 70-71 0.2774245540103415 2208 72-73 0.4855484802939145 2208 74-75 0.4592666670471033 2208 76-77 0.384946512037434 2208 78-79 0.3942987494899235 2208 80-81 0.46586987307486183 2208 82-83 0.5697261319939351 2208 84-85 0.5740259745219163 2208 86-87 0.6100296611907652 2208 88-89 0.5279810086978145 2208 90-91 0.4858692886009379 2208 92-93 0.39317455044124827 2208 94-95 0.48162292062662715 2208 96-97 0.35228549917700036 2208 98-99 0.41949748965797795 2208 100-101 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-2.7586897532930195 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37930.0 3 6297.0 4 4709.0 5 3347.0 6 4163.0 7 3608.0 8 4008.0 9 4661.0 10 5968.0 11 7021.0 12 7783.0 13 8704.0 14 9167.0 15 9248.0 16 9286.0 17 9141.0 18 9438.0 19 9964.0 20 10142.0 21 10840.0 22 12101.0 23 15113.0 24 17002.0 25 17729.0 26 20179.0 27 21403.0 28 24390.0 29 30307.0 30 35536.0 31 42680.0 32 53825.0 33 66966.0 34 80439.0 35 96184.0 36 124476.0 37 185729.0 38 87205.0 39 636.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.80101895891585 16.927350625142367 9.506501898069624 22.765128517872167 2 12.337784311323452 24.295352561921224 36.278419789383896 27.088443337371427 3 22.715568628660705 32.733914122764205 26.270076158297478 18.280441090277613 4 13.397674884928307 19.466147191960363 32.007759456838016 35.12841846627331 5 10.765734091249337 43.27615694849589 32.300922292438834 13.65718666781594 6 30.59857305019368 28.895597470580388 21.671743472063053 18.834086007162878 7 25.065436179198507 33.92011932292947 21.883037762912817 19.131406734959207 8 29.96135926805851 33.00515633252446 21.984380359836145 15.049104039580888 9 32.38257485339726 16.152305300389276 18.776678250877893 32.688441595335576 10-11 23.32341417965483 31.416608538943606 23.811233225972902 21.44874405542866 12-13 27.209381481195738 28.408615423714203 23.053020435763266 21.328982659326794 14-15 28.321084692117342 26.389825294661563 22.48434022033943 22.804749792881662 16-17 23.14306144634946 29.673766472293156 29.361924932985602 17.821247148371782 18-19 25.492164607881072 28.612847747554433 28.251552765444522 17.643434879119972 20-21 21.523013850777474 29.531175138289996 29.035726730258286 19.910084280674244 22-23 20.11238285133708 31.912037707201062 22.11785814603236 25.857721295429492 24-25 20.83947939754581 33.44614414344925 21.174236666764973 24.540139792239966 26-27 23.957466909707712 27.580393902555787 22.404518131197694 26.057621056538803 28-29 23.229163027217258 28.006555773304914 25.786540790495216 22.97774040898261 30-31 26.25158038713472 29.967679778396366 24.171731922776363 19.60900791169255 32-33 19.146581256117745 30.497528851245963 27.266906301463358 23.088983591172934 34-35 16.82063815392127 32.108352849194766 28.23479657295272 22.836212423931247 36-37 24.245970404882687 29.087517145283144 24.91159746866677 21.754914981167403 38-39 27.18396638973991 27.218833497996158 24.024299064573068 21.57290104769086 40-41 21.68954533131069 31.761749363238962 24.17864853420284 22.37005677124751 42-43 25.352514997671737 29.536447142133632 23.315751602011144 21.795286258183484 44-45 27.382024744845427 27.183044546708867 23.60997341185499 21.824957296590714 46-47 22.83748764502215 28.927726271298305 29.993418238510156 18.24136784516939 48-49 25.169257119322303 28.292088391996135 28.17401890744677 18.364635581234797 50-51 20.206936650028165 29.888213935524366 29.11009258360598 20.794756830841486 52-53 19.267202948856674 31.967322401496922 22.615640949126963 26.149833700519444 54-55 20.278263030626256 33.11428929051499 21.56631456357175 25.041133115287007 56-57 23.437264074587457 27.603063555953973 22.632108028843604 26.327564340614966 58-59 22.955803873254286 27.723074425629918 25.773342662251302 23.547779038864498 60-61 25.47192485844295 29.855409098288614 24.400689668308 20.27197637496043 62-63 18.888288257238038 30.382624532557912 27.131866965732854 23.59722024447119 64-65 16.554932213222084 31.651442932427305 28.202619670615814 23.5910051837348 66-67 23.423763357812735 29.01795614750771 25.04082797917263 22.51745251550692 68-69 26.16470842209192 27.300334565257913 24.186977397683666 22.3479796149665 70-71 21.15986899188978 31.053466857225015 24.431851426171413 23.35481272471379 72-73 24.55528346242764 29.186213753317315 23.746064691237294 22.51243809301775 74-75 26.368405630654536 26.852519633537536 24.036421266724908 22.742653469083017 76-77 22.054060575384053 28.405137283128852 30.17362391448202 19.36717822700507 78-79 24.237960566057932 28.03523258914723 28.33364474622268 19.393162098572155 80-81 19.622484735840004 29.589883701206166 29.176111805039646 21.611519757914188 82-83 18.728201741774374 31.325270604541384 23.038100067214604 26.908427586469635 84-85 19.73661653337397 32.361915750307716 22.171124420727807 25.7303432955905 86-87 22.466132552481614 27.16185619193277 23.34041796612076 27.03159328946485 88-89 22.124915591751144 27.04365465382528 26.338910966927386 24.492518787496195 90-91 24.376725648626195 29.069174700041 25.000045352132094 21.554054299200715 92-93 18.56504756081852 29.512245345970257 27.34139287975631 24.581314213454913 94-95 16.590288341783026 30.535274177322897 28.48226653132645 24.392170949567628 96-97 22.919272822900773 28.060657034751518 25.73045297810262 23.289617164245087 98-99 25.047515483044236 26.796873973028703 24.855685743723267 23.299924800203797 100-101 20.585316943177638 29.611249790922617 25.118522510777968 24.684910755121773 102-103 23.55177078683257 28.235965928017386 24.64676037443831 23.565502910711736 104-105 24.89010832131198 26.26958369646647 24.896543878946254 23.943764103275292 106-107 21.118636907184904 27.69607287621904 30.3366844330253 20.848605783570758 108-109 22.94011595198904 27.801413745691693 28.462908555323807 20.79556174699546 110-111 18.79918622402598 29.26886663060565 29.40234983026686 22.52959731510151 112-113 18.165248923430983 30.636402133098294 23.803895325482472 27.394453617988248 114-115 18.776063509047138 32.28347848952926 22.977916007380976 25.962541994042628 116-117 21.22876389338366 27.53552835424196 23.9483362386947 27.287371513679677 118-119 20.76203764013448 27.561700900090173 26.41517906551009 25.261082394265262 120-121 22.636415225032785 29.3568476494227 25.43029012007706 22.57644700546745 122-123 17.946584631009568 29.828001695725998 27.11066879161386 25.11474488165057 124-125 16.498939777455277 30.813326931647495 27.933293708167454 24.754439582729777 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6416.0 1 4026.0 2 2609.5 3 3707.5 4 3524.5 5 2793.0 6 2075.0 7 1700.0 8 1556.0 9 1440.5 10 1271.5 11 1117.5 12 1036.0 13 1033.5 14 1085.0 15 1123.5 16 1165.0 17 1238.0 18 1337.5 19 1490.0 20 1776.0 21 2105.0 22 2519.0 23 2990.5 24 3489.5 25 4200.0 26 5060.5 27 6045.5 28 7031.0 29 8104.0 30 9519.0 31 10860.5 32 12157.0 33 13831.5 34 15501.5 35 16373.5 36 17765.5 37 19430.5 38 19573.5 39 19125.0 40 18809.5 41 19055.0 42 19501.5 43 20356.0 44 21409.0 45 22182.0 46 24609.5 47 31656.0 48 42214.0 49 71981.0 50 169927.0 51 178120.0 52 75487.5 53 30737.5 54 20630.0 55 16346.0 56 13603.0 57 11644.0 58 10400.5 59 9450.0 60 8520.0 61 7547.0 62 6477.0 63 5034.0 64 3658.5 65 2835.5 66 1944.0 67 1461.0 68 1171.0 69 775.0 70 620.0 71 582.0 72 570.5 73 577.0 74 395.5 75 294.5 76 229.0 77 119.5 78 88.5 79 57.0 80 40.5 81 34.5 82 22.0 83 22.0 84 22.5 85 18.0 86 18.0 87 15.5 88 10.5 89 11.5 90 12.5 91 6.5 92 2.5 93 3.0 94 4.5 95 3.0 96 0.5 97 1.5 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48910663084460293 2 0.4804370893820694 3 0.4648138532047953 4 0.4683358544239496 5 0.46779400808254124 6 0.4744767796265776 7 0.46111123653850494 8 0.48576524507258484 9 0.5001241731199061 10-11 0.5043234822658208 12-13 0.5039170975097645 14-15 0.47845031946357214 16-17 0.48070801255277357 18-19 0.46765854649718913 20-21 0.4779987808457318 22-23 0.4936671708847899 24-25 0.48156593592667013 26-27 0.4924931704784052 28-29 0.4872553225114578 30-31 0.5001241731199061 32-33 0.4975504029982164 34-35 0.49159009324272457 36-37 0.44982277109249763 38-39 0.41239021967353756 40-41 0.4043979861377644 42-43 0.4127514505678098 44-45 0.4473844625561601 46-47 0.45483484975052496 48-49 0.45226107962883527 50-51 0.4390761519878988 52-53 0.43311584223240696 54-55 0.43410922719165557 56-57 0.4171765290226446 58-59 0.42087914568893503 60-61 0.43040661052536516 62-63 0.43185153410245414 64-65 0.44562346194658303 66-67 0.4365023818662091 68-69 0.4379473054432981 70-71 0.4470683855236719 72-73 0.44842300137719276 74-75 0.45171923328742686 76-77 0.4437269997516538 78-79 0.4355541507687445 80-81 0.44304969182489334 82-83 0.44124353735353217 84-85 0.4392567674350349 86-87 0.44602984670263923 88-89 0.43316099609419095 90-91 0.43717968979296956 92-93 0.44675230849118375 94-95 0.44413338450771 96-97 0.4124805273971056 98-99 0.385026979432416 100-101 0.38511728715598403 102-103 0.36777820423091684 104-105 0.36841035829589325 106-107 0.38868444223692233 108-109 0.394509290407062 110-111 0.41220960422640146 112-113 0.40782967963335065 114-115 0.40548167882058117 116-117 0.3785699771972998 118-119 0.4507258483281783 120-121 0.4582665432461111 122-123 0.41112591154358474 124-125 0.4055268326823651 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1107325.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.462290948608114 #Duplication Level Percentage of deduplicated Percentage of total 1 88.55118949420222 47.34149456584369 2 8.045190366275804 8.602286161975522 3 1.7148049336484912 2.750322008484728 4 0.5901798449902731 1.2620946633949768 5 0.2812505773792798 0.7518150098657537 6 0.17290241599258552 0.5546255561707728 7 0.11769381965387286 0.4404526860431853 8 0.07941801211540661 0.33966950962191617 9 0.06331641398505439 0.3046536491662348 >10 0.3306815670340387 3.232351054414697 >50 0.028387004230009363 1.0576022480262666 >100 0.017336912640497977 1.912534830053366 >500 0.002379576220784689 0.8056508631088045 >1k 0.002039636760672591 3.2095182139637912 >5k 0.001529727570504443 5.051356398528687 >10k+ 0.0016996973005604925 22.383572581337624 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 66437 5.999774230691079 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 48134 4.346871966224911 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 31464 2.841442214345382 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 19633 1.7730115368116857 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 17758 1.6036845551215768 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 14886 1.3443207730341138 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 14196 1.2820084437721537 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 12892 1.1642471722394057 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 10697 0.9660217190075182 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 10229 0.9237577043776668 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 7327 0.6616846905831621 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 7006 0.6326959113178154 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 6791 0.613279750750683 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 6211 0.5609012710812092 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6073 0.5484388052288172 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 6058 0.5470841893752962 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5522 0.49867924954281717 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 5447 0.4919061702752128 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 5154 0.46544600726977176 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 4913 0.4436818458898697 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 4414 0.3986182918294087 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 4308 0.38904567313119454 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4189 0.3782990540265956 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 4026 0.3635788950850022 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3056 0.2759804032239857 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3035 0.27408394102905653 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2147 0.1938906825006209 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 1807 0.1631860564874811 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 1254 0.11324588535434493 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1153 0.10412480527397107 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.030772356805816E-5 0.0 10-11 9.030772356805816E-5 0.0 0.0 9.030772356805816E-5 0.0 12-13 9.030772356805816E-5 0.0 0.0 4.966924796243198E-4 0.0 14-15 9.030772356805816E-5 0.0 0.0 0.0014449235770889305 0.0 16-17 9.030772356805816E-5 0.0 0.0 0.003386539633802181 0.0 18-19 1.8061544713611632E-4 0.0 0.0 0.0047863093491070825 0.0 20-21 2.709231707041745E-4 0.0 0.0 0.006908540852956449 0.0 22-23 2.709231707041745E-4 0.0 0.0 0.011198157722439211 0.0 24-25 2.709231707041745E-4 0.0 0.0 0.01571354390084212 0.0 26-27 2.709231707041745E-4 0.0 0.0 0.020183776217460998 0.0 28-29 4.0638475605626174E-4 0.0 0.0 0.02785993272074594 0.0 30-31 4.515386178402908E-4 0.0 0.0 0.04172216828844287 0.0 32-33 4.515386178402908E-4 0.0 0.0 0.06791140812317974 0.0 34-35 4.515386178402908E-4 0.0 0.0 0.1074661910459892 0.0 36-37 5.41846341408349E-4 0.0 0.0 0.16232813311358454 0.0 38-39 5.41846341408349E-4 0.0 0.0 0.24933962477140859 0.0 40-41 5.41846341408349E-4 0.0 0.0 0.38480121012349583 0.0 42-43 5.41846341408349E-4 0.0 0.0 0.4842751676337119 0.0 44-45 5.41846341408349E-4 0.0 0.0 0.5769760458763236 0.0 46-47 6.321540649764071E-4 0.0 0.0 0.7016007044002438 0.0 48-49 6.321540649764071E-4 0.0 0.0 0.8189104373151515 0.0 50-51 6.321540649764071E-4 0.0 0.0 0.9407355564084618 0.0 52-53 6.321540649764071E-4 0.0 0.0 1.1003093039532206 0.0 54-55 6.321540649764071E-4 0.0 0.0 1.2586187433680265 0.0 56-57 7.224617885444653E-4 0.0 0.0 1.4250107240421737 0.0 58-59 7.224617885444653E-4 0.0 0.0 1.6254938703632629 0.0 60-61 7.224617885444653E-4 0.0 0.0 1.8115729347752465 0.0 62-63 7.224617885444653E-4 0.0 0.0 1.9971101528458222 0.0 64-65 7.224617885444653E-4 0.0 0.0 2.212539227417425 0.0 66-67 7.224617885444653E-4 0.0 0.0 2.4588535434493037 0.0 68-69 7.224617885444653E-4 0.0 0.0 2.7650870340685887 0.0 70-71 7.224617885444653E-4 0.0 0.0 3.225295193371413 0.0 72-73 7.224617885444653E-4 0.0 0.0 3.583500778904116 0.0 74-75 8.127695121125235E-4 0.0 0.0 3.913214277651096 0.0 76-77 9.030772356805816E-4 0.0 0.0 4.303975797530084 0.0 78-79 9.030772356805816E-4 0.0 0.0 4.669631770257151 0.0 80-81 9.030772356805816E-4 9.030772356805816E-5 0.0 5.057322827534826 0.0 82-83 9.933849592486397E-4 9.030772356805816E-5 0.0 5.547151920167972 0.0 84-85 0.0010385388210326688 9.030772356805816E-5 0.0 6.013184927640936 0.0 86-87 0.001083692682816698 9.030772356805816E-5 0.0 6.473664010114465 0.0 88-89 0.001083692682816698 9.030772356805816E-5 0.0 7.018580814124128 0.0 90-91 0.001174000406384756 9.030772356805816E-5 0.0 7.5248007585848775 0.0 92-93 0.001174000406384756 9.030772356805816E-5 0.0 8.030207933533516 0.0 94-95 0.001174000406384756 9.030772356805816E-5 0.0 8.586458356850969 0.0 96-97 0.001174000406384756 9.030772356805816E-5 0.0 9.139141625087486 0.0 98-99 0.001219154268168785 9.030772356805816E-5 0.0 9.785519156525861 0.0 100-101 0.0013546158535208725 9.030772356805816E-5 0.0 10.748696182240986 0.0 102-103 0.0014449235770889305 9.030772356805816E-5 0.0 11.512834985212109 0.0 104-105 0.0015352313006569886 9.030772356805816E-5 0.0 12.205991917458741 0.0 106-107 0.0015352313006569886 9.030772356805816E-5 0.0 12.947598943399635 0.0 108-109 0.0015352313006569886 9.030772356805816E-5 0.0 13.595782629309372 0.0 110-111 0.0015352313006569886 9.030772356805816E-5 0.0 14.280360327817036 0.0 112-113 0.001625539024225047 9.030772356805816E-5 0.0 15.1379676246811 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCACGC 50 7.4246364E-6 59.536644 3 GTCGCGA 15 0.004228269 59.531242 22-23 TATCACG 55 1.3076584E-5 54.111942 2 AGTACTC 1280 0.0 48.375717 5 AAGTACT 1490 0.0 41.156204 4 TATAACG 135 1.3205863E-9 39.68209 2 ATAACGC 145 2.6520866E-9 36.953777 3 AAAAAGT 1765 0.0 33.721096 1 AAAGTAC 1755 0.0 33.584778 3 TCACGCA 90 2.3842951E-4 33.075912 4 TGTATCG 75 0.0038995151 31.751434 9 AAAAGTA 2015 0.0 29.54002 2 GTACAAA 910 0.0 27.469725 1 GTACAAG 625 0.0 26.663946 1 GAACAAA 1260 0.0 26.452326 1 CATGGGG 4160 0.0 24.329878 4 ATGGGAG 2235 0.0 24.241972 5 TAACGCA 225 1.9003164E-7 23.814657 4 GTACATA 625 0.0 23.807095 1 GTCGGGA 335 3.45608E-11 23.09853 2 >>END_MODULE