##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364162_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1107325 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06110220576615 33.0 31.0 34.0 30.0 34.0 2 31.851131330007 33.0 31.0 34.0 30.0 34.0 3 32.097485381437245 34.0 31.0 34.0 30.0 34.0 4 35.54152665206692 37.0 35.0 37.0 33.0 37.0 5 35.69311268146208 37.0 35.0 37.0 33.0 37.0 6 35.86317296186756 37.0 35.0 37.0 35.0 37.0 7 35.832387058903215 37.0 35.0 37.0 35.0 37.0 8 35.89206691802316 37.0 35.0 37.0 35.0 37.0 9 37.54233761542456 39.0 37.0 39.0 35.0 39.0 10-11 37.50007540694918 39.0 37.0 39.0 34.5 39.0 12-13 37.48659291535908 39.0 37.0 39.0 34.5 39.0 14-15 38.74143905357506 40.0 38.0 41.0 34.0 41.0 16-17 38.71847470254893 40.0 38.0 41.0 34.0 41.0 18-19 38.78905289774908 40.0 38.0 41.0 34.0 41.0 20-21 38.82290926331474 40.0 38.0 41.0 35.0 41.0 22-23 38.7702607635518 40.0 38.0 41.0 34.5 41.0 24-25 38.740552683268234 40.0 38.0 41.0 34.5 41.0 26-27 38.521403382024246 40.0 38.0 41.0 34.0 41.0 28-29 38.33945002596347 40.0 38.0 41.0 34.0 41.0 30-31 38.15574199083377 40.0 37.5 41.0 34.0 41.0 32-33 37.760184679294696 40.0 37.0 41.0 33.0 41.0 34-35 37.48426884609306 40.0 36.0 41.0 33.0 41.0 36-37 37.35377915246201 40.0 36.5 41.0 33.0 41.0 38-39 37.23751834375635 40.0 36.0 41.0 32.5 41.0 40-41 36.95694308355722 40.0 36.0 41.0 31.0 41.0 42-43 36.7612327004267 40.0 35.5 41.0 31.0 41.0 44-45 36.484523513873526 40.0 35.0 41.0 30.5 41.0 46-47 36.07595511706138 39.0 35.0 41.0 30.0 41.0 48-49 35.67193597182399 39.0 35.0 41.0 27.5 41.0 50-51 35.516045876323574 39.0 35.0 41.0 27.0 41.0 52-53 35.430489693631046 39.0 35.0 41.0 27.0 41.0 54-55 35.24308491183709 39.0 35.0 41.0 26.0 41.0 56-57 34.93420675953311 39.0 35.0 41.0 25.0 41.0 58-59 34.755975887837806 38.5 35.0 40.0 24.0 41.0 60-61 34.5680157135439 38.0 34.5 40.0 25.0 41.0 62-63 34.17624003792925 38.0 34.0 40.0 22.5 41.0 64-65 33.912592960512946 37.5 34.0 40.0 22.0 41.0 66-67 33.44406700833089 37.0 33.5 39.5 22.0 41.0 68-69 33.10472128778814 36.0 33.0 39.0 21.0 41.0 70-71 32.58045605400402 36.0 33.0 39.0 19.5 40.5 72-73 32.21154584245818 35.0 32.0 38.0 18.5 39.0 74-75 31.875090420608224 35.0 32.0 37.0 18.0 39.0 76-77 31.340322398573136 35.0 32.0 36.5 14.0 39.0 78-79 30.93038042128553 35.0 31.5 36.0 11.0 37.0 80-81 30.6596152890976 35.0 31.0 36.0 7.5 37.0 82-83 30.489814643397374 35.0 31.0 35.0 7.0 36.5 84-85 30.258317567109927 35.0 31.0 35.0 4.0 36.0 86-87 29.945870453570542 35.0 31.0 35.0 2.0 36.0 88-89 29.73355699546204 34.0 31.0 35.0 2.0 36.0 90-91 29.55922696588626 34.0 31.0 35.0 2.0 35.5 92-93 29.300756778723503 34.0 30.0 35.0 2.0 35.0 94-95 29.225328155690516 34.0 30.0 35.0 2.0 35.0 96-97 29.15278305827106 34.0 30.0 35.0 2.0 35.0 98-99 29.008646964531643 34.0 30.0 35.0 2.0 35.0 100-101 28.711299302372836 34.0 29.0 35.0 2.0 35.0 102-103 27.240705303321064 32.5 26.0 34.5 2.0 35.0 104-105 28.141034023434855 34.0 29.0 35.0 2.0 35.0 106-107 28.146707606168018 34.0 28.0 35.0 2.0 35.0 108-109 28.116798139660894 34.0 29.0 35.0 2.0 35.0 110-111 28.07315061070598 34.0 29.0 35.0 2.0 35.0 112-113 28.0168058158174 34.0 29.0 35.0 2.0 35.0 114-115 27.908203102070303 34.0 29.0 35.0 2.0 35.0 116-117 27.598671573386312 34.0 27.0 35.0 2.0 35.0 118-119 27.38224775923961 34.0 27.0 35.0 2.0 35.0 120-121 27.16412706296706 34.0 27.0 35.0 2.0 35.0 122-123 26.74629173910099 34.0 25.0 35.0 2.0 35.0 124-125 25.629937913440045 32.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2891614803161886 1101 2 0.6041066997636513 1101 3 0.4219038642465591 1101 4 0.5302514959813251 1101 5 0.26949137199248696 1101 6 0.23407418690543125 1101 7 0.21402946841669035 1101 8 0.18505493184602528 1101 9 0.17706987350647552 1101 10-11 0.2381880610058147 1101 12-13 0.23562265345168498 1101 14-15 0.3660733553862343 1101 16-17 0.4107740742366346 1101 18-19 0.2927339773964377 1101 20-21 0.169382949770565 1101 22-23 0.15458046345439413 1101 24-25 0.21641938753887047 1101 26-27 0.3790721544271207 1101 28-29 0.36737205574599585 1101 30-31 0.37799035879876897 1101 32-33 0.3044452911119677 1101 34-35 0.19911780471716156 1101 36-37 0.21524458794714718 1101 38-39 0.22259277821530077 1101 40-41 0.3340018853349136 1101 42-43 0.3623446635524985 1101 44-45 0.48078866135632126 1101 46-47 0.4725536852278651 1101 48-49 0.366181844616392 1101 50-51 0.28390415977955996 1101 52-53 0.2053097994252937 1101 54-55 0.30366005689587183 1101 56-57 0.41018044340140136 1101 58-59 0.4902988990289998 1101 60-61 0.382457960252907 1101 62-63 0.3180978792388842 1101 64-65 0.38520204349059384 1101 66-67 0.32440071452341357 1101 68-69 0.3136162985784594 1101 70-71 0.28923285135041965 1101 72-73 0.291990456818894 1101 74-75 0.2666816232171527 1101 76-77 0.2702011494968062 1101 78-79 0.17255041463094756 1101 80-81 0.051056986546981875 1101 82-83 0.07941330437828142 1101 84-85 0.3277033345431768 1101 86-87 0.3491728553283764 1101 88-89 0.30311178321923293 1101 90-91 0.3247676604090408 1101 92-93 0.3386186928821324 1101 94-95 0.4400976895059472 1101 96-97 0.34690851727372873 1101 98-99 0.26393327752507645 1101 100-101 0.2518124945044704 1101 102-103 0.2957450422311396 1101 104-105 0.5686654397228423 1101 106-107 0.302979334810896 1101 108-109 0.29825419698158484 1101 110-111 0.08997638567635491 1101 112-113 0.24918526687601883 1101 114-115 0.4815300769019757 1101 116-117 0.579124815474497 1101 118-119 0.48960128953882887 1101 120-121 0.4721530908389404 1101 122-123 0.45387329572896107 1101 124-125 0.7299986296358263 1102 1 0.23727422593050562 1102 2 0.5956736536047309 1102 3 0.4084553359461509 1102 4 0.5233435673288866 1102 5 0.31023348005447104 1102 6 0.23625155359992078 1102 7 0.21571792537988443 1102 8 0.13990302799901144 1102 9 0.3185632322054701 1102 10-11 0.19842063126303344 1102 12-13 0.24991947524279112 1102 14-15 0.3752094633469696 1102 16-17 0.2944678814909025 1102 18-19 0.24726075305708406 1102 20-21 0.2309761524840681 1102 22-23 0.2442687918154789 1102 24-25 0.329677747322485 1102 26-27 0.28847104674909474 1102 28-29 0.3333763846111424 1102 30-31 0.36174528900810543 1102 32-33 0.35398444842080323 1102 34-35 0.22586519507370184 1102 36-37 0.23864536717243823 1102 38-39 0.23470133169658425 1102 40-41 0.3249773803513989 1102 42-43 0.3880211087833345 1102 44-45 0.4886211948067327 1102 46-47 0.3887766191962214 1102 48-49 0.47155353241926434 1102 50-51 0.48577768552086553 1102 52-53 0.5627440174154685 1102 54-55 0.5744988641335098 1102 56-57 0.5850144524501104 1102 58-59 0.6735136634377028 1102 60-61 0.6498703343623475 1102 62-63 0.520422436217558 1102 64-65 0.5554852748476264 1102 66-67 0.525416708116957 1102 68-69 0.4061889356718211 1102 70-71 0.5067123165873895 1102 72-73 0.48941113463329344 1102 74-75 0.48197947197738245 1102 76-77 0.4302958050815171 1102 78-79 0.49325709059539946 1102 80-81 0.4719803644512943 1102 82-83 0.521651187971937 1102 84-85 0.5015353188478038 1102 86-87 0.46876597748079263 1102 88-89 0.43544020934748673 1102 90-91 0.4754021159435702 1102 92-93 0.4582578235466741 1102 94-95 0.45337807424797205 1102 96-97 0.5785519524133669 1102 98-99 0.45670007423507997 1102 100-101 0.4282963429298903 1102 102-103 0.43898617007160823 1102 104-105 0.5978989526926242 1102 106-107 0.6746518015064602 1102 108-109 0.6466572581551695 1102 110-111 0.5273368126649096 1102 112-113 0.4331149185675578 1102 114-115 0.5370486778293113 1102 116-117 0.4888614730983676 1102 118-119 0.41950630505792574 1102 120-121 0.40868037573435956 1102 122-123 0.4503598344513122 1102 124-125 0.650225967117688 1103 1 0.16395265226034894 1103 2 0.5060405270614616 1103 3 0.3164285941474816 1103 4 0.4893748138015468 1103 5 0.2434140262959943 1103 6 0.1919657982112497 1103 7 0.18902076031449155 1103 8 0.12899546086971014 1103 9 0.16174768112065152 1103 10-11 0.18275202358796605 1103 12-13 0.182029183899715 1103 14-15 0.34915985438896513 1103 16-17 0.3758911917401804 1103 18-19 0.1783379868951087 1103 20-21 0.09068185842904342 1103 22-23 -0.03061849911667025 1103 24-25 -0.02657733851051347 1103 26-27 -0.05093393200014873 1103 28-29 0.060723724780430643 1103 30-31 0.05527363798533713 1103 32-33 0.09496366539249124 1103 34-35 0.17711884599172123 1103 36-37 0.200454680619103 1103 38-39 0.1335630641738419 1103 40-41 0.16357508109378927 1103 42-43 0.2301859934257635 1103 44-45 0.15449773604928652 1103 46-47 0.2144366616460971 1103 48-49 0.3769111139714951 1103 50-51 0.25283749676130185 1103 52-53 0.06308356477909882 1103 54-55 0.18772970248536325 1103 56-57 0.1871176910790524 1103 58-59 0.205102081128544 1103 60-61 0.2797520885145701 1103 62-63 0.13480286648548656 1103 64-65 0.21742705049774713 1103 66-67 0.15455260157470718 1103 68-69 0.3202841904190237 1103 70-71 0.181177875307867 1103 72-73 0.20647338043385588 1103 74-75 0.2620167797670163 1103 76-77 0.14315493036619742 1103 78-79 0.11933226243476724 1103 80-81 0.11458712175737062 1103 82-83 0.08550342935341249 1103 84-85 0.1290253021083565 1103 86-87 -0.023942330492541686 1103 88-89 -0.00424928095153021 1103 90-91 0.14467726624077315 1103 92-93 0.12554714741030537 1103 94-95 0.11607253473837886 1103 96-97 0.07451334914264862 1103 98-99 0.023268141216469473 1103 100-101 -0.06334444731357536 1103 102-103 -0.03805592789951717 1103 104-105 0.27673694451987885 1103 106-107 0.16855249063452504 1103 108-109 0.131707952336793 1103 110-111 0.25681992603942305 1103 112-113 0.08443684783104644 1103 114-115 0.1288291970693436 1103 116-117 0.11869176950237659 1103 118-119 0.11082861057161253 1103 120-121 0.19434041080678455 1103 122-123 0.23700512863101864 1103 124-125 0.2346072679252984 1104 1 0.25060520608499814 1104 2 0.5583691089725882 1104 3 0.10324137981961456 1104 4 0.4633341768133121 1104 5 0.17195869314480916 1104 6 0.19154337552644307 1104 7 0.14828270162254142 1104 8 0.14799374526346298 1104 9 0.2413478863714289 1104 10-11 0.27078920566954423 1104 12-13 0.17680276756199476 1104 14-15 0.3043588002944233 1104 16-17 0.31191858960904284 1104 18-19 0.22490777401036866 1104 20-21 0.17671262531317922 1104 22-23 0.15024875205563148 1104 24-25 0.12195365976202766 1104 26-27 0.16082718926737272 1104 28-29 0.3231888961966689 1104 30-31 0.2595429578070636 1104 32-33 0.2765382246623531 1104 34-35 0.2505946627557947 1104 36-37 0.38479331330240285 1104 38-39 0.4934816302617051 1104 40-41 0.5330766445390083 1104 42-43 0.4475704084351335 1104 44-45 0.4351152358253003 1104 46-47 0.48305972659108676 1104 48-49 0.5540102511333629 1104 50-51 0.4754076037478967 1104 52-53 0.5190836699219972 1104 54-55 0.5710057255368284 1104 56-57 0.6775626454418529 1104 58-59 0.6649623003144569 1104 60-61 0.45073090962578277 1104 62-63 0.3748533498836437 1104 64-65 0.19005991002165246 1104 66-67 0.3781565519857608 1104 68-69 0.4342844480931589 1104 70-71 0.28490967059317995 1104 72-73 0.22079854446047165 1104 74-75 0.2815824285468054 1104 76-77 0.3262109308045389 1104 78-79 0.2611537372045447 1104 80-81 0.2507032987321125 1104 82-83 0.26485943652978605 1104 84-85 0.32212707672789875 1104 86-87 0.25274693449198793 1104 88-89 0.3538598277551195 1104 90-91 0.27994896806736946 1104 92-93 0.23744695363639678 1104 94-95 0.27560262744923847 1104 96-97 0.27910938940200936 1104 98-99 0.18121331586104006 1104 100-101 0.16934177593144994 1104 102-103 0.03693390952143005 1104 104-105 0.21106410033794987 1104 106-107 0.29760317617360244 1104 108-109 0.22609702399167375 1104 110-111 -0.02209936790382727 1104 112-113 -0.03857589593201638 1104 114-115 -0.04526429717704161 1104 116-117 0.0036110204999388884 1104 118-119 -0.18530838765798663 1104 120-121 -0.1503211438419747 1104 122-123 0.04902462745482694 1104 124-125 -0.0016023529457136476 1105 1 0.13751449887365652 1105 2 0.5214796591318738 1105 3 0.15374873038486925 1105 4 0.3588062980136044 1105 5 0.18829280067840415 1105 6 0.19584810248531426 1105 7 0.17170713902617507 1105 8 0.06291629270762655 1105 9 0.1267586570802024 1105 10-11 0.18378872206545793 1105 12-13 0.12841850399559718 1105 14-15 0.2490105294501177 1105 16-17 0.2420432206711709 1105 18-19 0.20807217120763255 1105 20-21 0.1174244992031177 1105 22-23 0.12334041346338154 1105 24-25 0.1122631446911484 1105 26-27 0.12551921153484358 1105 28-29 0.24895458459515396 1105 30-31 0.3398835842370005 1105 32-33 0.32704503307056143 1105 34-35 0.24082553491299308 1105 36-37 0.39144070086445026 1105 38-39 0.32721019608281665 1105 40-41 0.48307892159861865 1105 42-43 0.4690219363421235 1105 44-45 0.3843633724315296 1105 46-47 0.3808164975904589 1105 48-49 0.5483479629270107 1105 50-51 0.5559808155513082 1105 52-53 0.3792938967388153 1105 54-55 0.438828379460638 1105 56-57 0.4436056146408305 1105 58-59 0.4820623673250637 1105 60-61 0.6038268432310616 1105 62-63 0.6520728866212906 1105 64-65 0.6271398738489324 1105 66-67 0.5572971680062864 1105 68-69 0.5533237024320385 1105 70-71 0.7506968500970359 1105 72-73 0.765749327443686 1105 74-75 0.58950323458253 1105 76-77 0.4802491333587504 1105 78-79 0.6277353936461267 1105 80-81 0.6247097959421701 1105 82-83 0.7008144244830525 1105 84-85 0.6562850309308139 1105 86-87 0.5932719810632747 1105 88-89 0.6447682877905514 1105 90-91 0.5602484989930936 1105 92-93 0.5383462837471491 1105 94-95 0.5230647903088794 1105 96-97 0.5170182029238362 1105 98-99 0.5137757018725182 1105 100-101 0.3831533351534304 1105 102-103 0.20306709365314646 1105 104-105 0.2661515936423058 1105 106-107 0.29270587156158 1105 108-109 0.28287062571443045 1105 110-111 0.3105877142286282 1105 112-113 0.36789550745234223 1105 114-115 0.37052515938642117 1105 116-117 0.3620370084727256 1105 118-119 0.1465264235954571 1105 120-121 0.18883441402678258 1105 122-123 0.23422222201243414 1105 124-125 0.28944706792212926 1106 1 0.1809341621441618 1106 2 0.43304034548325276 1106 3 0.32451958994965224 1106 4 0.45599456678341 1106 5 0.08713942262811258 1106 6 0.1229262464582277 1106 7 0.09959421013538616 1106 8 0.012837081609525569 1106 9 0.21782269229883156 1106 10-11 0.11771436943488567 1106 12-13 0.13260187295816195 1106 14-15 0.13591365228022312 1106 16-17 0.17719241850552692 1106 18-19 0.07983970327568812 1106 20-21 -0.01709072957716984 1106 22-23 -0.0803820527098722 1106 24-25 -0.10109007374580159 1106 26-27 0.07576423693073764 1106 28-29 0.08923509207869529 1106 30-31 0.06600254171945608 1106 32-33 0.14105923151100086 1106 34-35 0.09673262985909759 1106 36-37 0.26383498505327196 1106 38-39 0.21626368476940172 1106 40-41 0.31389438601527786 1106 42-43 0.22567237541894514 1106 44-45 0.2591420473044792 1106 46-47 0.1931618677876088 1106 48-49 0.2482489578127982 1106 50-51 0.3323809531635149 1106 52-53 0.4530516558179656 1106 54-55 0.47267597184263366 1106 56-57 0.4439342303419309 1106 58-59 0.41967513190800076 1106 60-61 0.38931103289206703 1106 62-63 0.41562110239217986 1106 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-1.8231600073745398 2216 124-125 -2.170644924791457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31.0 3 17.0 4 59.0 5 205.0 6 595.0 7 1011.0 8 1698.0 9 3549.0 10 7438.0 11 9472.0 12 7945.0 13 9394.0 14 13450.0 15 13119.0 16 7615.0 17 6895.0 18 6903.0 19 6885.0 20 6955.0 21 7007.0 22 7252.0 23 7676.0 24 8621.0 25 9763.0 26 11469.0 27 13703.0 28 16925.0 29 20641.0 30 25429.0 31 32084.0 32 41366.0 33 54927.0 34 77210.0 35 121488.0 36 182206.0 37 258431.0 38 107118.0 39 773.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.801661936622835 17.632572366052937 10.779887455706712 22.785878241617514 2 12.059884911733151 23.95531931033735 36.201328608428966 27.783467169500526 3 22.972381089133144 30.479960192211653 28.563352451547473 17.98430626710773 4 13.594924705935474 19.285846522023796 32.91734585600434 34.20188291603639 5 10.40760391032443 40.02203508455061 35.2213668073962 14.348994197728759 6 30.999093276882896 28.69218737017784 20.83376983682596 19.474949516113302 7 21.821958322985573 33.051452825502906 24.891698462511005 20.23489038900052 8 28.4888357076739 31.528232452080463 24.14665974307453 15.836272097171111 9 29.768315535186147 13.829273248594587 22.25886708960784 34.14354412661143 10-11 23.247917278125215 27.848283024405664 26.6338699117242 22.269929785744928 12-13 27.631551615918504 25.6930816019652 26.533079253837155 20.142287528279137 14-15 28.291560007604044 22.649937167294844 27.046330032678423 22.01217279242269 16-17 21.823967301143213 26.521538096136876 33.708955971957444 17.945538630762464 18-19 24.203372993475266 24.183821371322782 34.281669789808774 17.331135845393177 20-21 20.08968464077152 24.762930830122514 35.58568423258042 19.561700296525544 22-23 18.89097600072246 26.826135055200595 29.43562188156142 24.84726706251552 24-25 20.125663197344952 28.436953920484047 28.160657440227578 23.276725441943423 26-27 22.727022328584653 23.05971598220938 29.66834488519631 24.544916804009663 28-29 21.7431294981733 22.635912056492923 33.35350206737049 22.267456377963278 30-31 24.569706274129093 24.851692140970357 31.567787235003276 19.010814349897277 32-33 18.197367529857992 25.085589145011628 33.97204975955568 22.744993565574696 34-35 16.17339813216451 26.923363476559942 35.18580145205854 21.717436939217006 36-37 23.27582236470774 24.583252432664302 30.93310455376696 21.207820648860995 38-39 25.191836181789444 22.952114329578038 30.686564468426162 21.169485020206352 40-41 20.93171423552897 26.19932097591989 30.811338501044634 22.05762628750651 42-43 24.45591854243334 24.954417176529024 29.783983925225204 20.80568035581243 44-45 26.512225408078027 22.167701442665884 29.773598537014877 21.546474612241212 46-47 21.92694105163344 24.571331813153318 35.59384101325266 17.90788612196058 48-49 24.489016323121035 24.30650441378999 33.01212381188901 18.192355451199965 50-51 19.939900209965458 25.589867473415662 33.523175219560656 20.947057097058224 52-53 19.052581672047502 27.627796717314247 27.511254600049668 25.80836701058858 54-55 20.455737926986206 28.78328404036755 26.18057029327433 24.58040773937191 56-57 23.01347842774253 23.816585013433276 27.506603752285912 25.663332806538282 58-59 22.28799133045854 23.25807689702662 30.964621949292216 23.48930982322263 60-61 24.78775427268417 25.490393515905446 29.25003950962906 20.47181270178132 62-63 18.748109182037794 25.70735782177771 31.715620978484182 23.82891201770031 64-65 16.66931569322466 27.10509561330233 33.13444562346194 23.091143070011064 66-67 23.229946041135168 25.161131555776308 29.163885941345136 22.44503646174339 68-69 25.1552163998826 23.618946560404577 28.923396473483393 22.302440566229425 70-71 21.185198564107196 26.43645722800442 29.177341792156774 23.201002415731605 72-73 24.445939538979072 25.302779220192807 28.243469622739486 22.00781161808864 74-75 26.264059783713 22.597746822296976 28.40737814101551 22.73081525297451 76-77 21.817036552051114 24.837694443817306 34.25561601155939 19.08965299257219 78-79 24.07481994897614 24.45040977129569 32.084573183121485 19.390197096606688 80-81 19.631002641500913 25.740184679294696 32.65667261192514 21.972140067279252 82-83 18.885873614340866 27.382295170794478 27.055561826925246 26.676269387939406 84-85 20.157677285349827 28.29268733208408 25.943693134355318 25.605942248210777 86-87 22.415415528413067 23.649606032555933 27.255277357596007 26.67970108143499 88-89 21.820829476440974 23.041293206601495 30.444946154019824 24.692931162937708 90-91 24.066014945928252 25.128214390535753 28.792089043415437 22.01368162012056 92-93 18.669902693427858 25.4506581175355 30.698485087937144 25.180954101099495 94-95 16.784203230336445 26.69850042670785 32.17151397750414 24.345782365451566 96-97 22.80371012744137 24.71300517884373 28.636310065940062 23.846974627774838 98-99 24.311993096562777 23.462474752195593 28.362208287104956 23.86332386413667 100-101 20.810114830837755 25.82625960140862 28.665896943488562 24.697728624265064 102-103 23.657613868915057 24.74130348005296 28.026329336158266 23.574753314873718 104-105 25.298072706300097 22.203780156728953 28.246800903826813 24.25134623314414 106-107 21.362806000212263 24.314363070197427 33.31915249147004 21.00367843812027 108-109 23.42609984567769 24.241429285650735 30.9579048296064 21.37456603906518 110-111 19.321751602769645 25.315495878592344 31.915031983135016 23.447720535503 112-113 18.656193027117567 26.72336124708206 26.860513900197848 27.759931825602518 114-115 19.557231577064364 27.80601596958587 25.792290582136395 26.844461871213365 116-117 21.664715203882686 23.530575627313972 26.902992423626433 27.90171674517691 118-119 21.006913315505436 23.276695663720293 29.556670714902854 26.15972030587142 120-121 23.061012696414167 24.878721561323854 28.2285896475948 23.83167609466718 122-123 18.362193687990274 25.298567849679305 29.673408676710444 26.665829785619977 124-125 16.749013820956122 26.613468388084932 30.800259007463172 25.83725878349577 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1691.0 1 1297.5 2 3074.5 3 4166.5 4 2048.5 5 1070.0 6 1567.5 7 3302.5 8 4972.5 9 5001.5 10 3681.5 11 2263.5 12 1783.5 13 1782.5 14 2089.5 15 2493.5 16 2630.0 17 2801.5 18 3001.5 19 3055.0 20 3164.5 21 3477.0 22 3907.5 23 4214.5 24 4466.0 25 5007.0 26 5783.5 27 6621.5 28 7317.5 29 8116.5 30 9339.0 31 10600.0 32 11878.5 33 13327.5 34 14663.0 35 15532.0 36 16666.5 37 17905.5 38 17826.0 39 17259.0 40 17086.5 41 17301.5 42 17686.0 43 18039.5 44 19002.5 45 20241.5 46 22900.5 47 30792.0 48 40885.0 49 73090.0 50 187073.5 51 187366.5 52 65550.5 53 26219.0 54 19542.5 55 14673.0 56 12142.0 57 10358.5 58 9413.5 59 8694.5 60 7897.5 61 6916.5 62 5866.5 63 4684.5 64 3504.0 65 2638.0 66 1750.0 67 1291.5 68 1014.0 69 705.0 70 568.5 71 508.0 72 516.5 73 487.5 74 326.5 75 245.0 76 170.0 77 90.5 78 45.5 79 16.5 80 8.0 81 6.0 82 5.0 83 1.5 84 1.5 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14693066624523063 2 9.030772356805816E-5 3 3.6123089427223264E-4 4 0.0 5 0.0 6 0.003702616666290384 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00541846341408349 14-15 0.0017610006095771342 16-17 7.224617885444653E-4 18-19 0.0 20-21 4.515386178402908E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 4.515386178402908E-5 30-31 0.0 32-33 0.0 34-35 4.515386178402908E-5 36-37 0.0 38-39 0.0 40-41 4.515386178402908E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 9.030772356805816E-4 96-97 0.002844693292393832 98-99 0.004560540040186937 100-101 4.515386178402908E-5 102-103 0.003883232113426501 104-105 0.022983315648070803 106-107 0.017835775404691487 108-109 0.020454699388165173 110-111 0.01616508251868241 112-113 0.014855620526945567 114-115 0.042534937800555395 116-117 0.04181247601201093 118-119 0.023344546542343032 120-121 0.014629851218025422 122-123 0.0035220012191542684 124-125 0.0018964621949292214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1107325.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.188631970967606 #Duplication Level Percentage of deduplicated Percentage of total 1 88.30837458111151 46.97001635549091 2 8.173992853748787 8.69526995262727 3 1.7272319944878427 2.7560732064988263 4 0.5906746238121952 1.2566870072214635 5 0.31020577339539734 0.8249710358198581 6 0.18353201488725102 0.5857090076836885 7 0.10983860253503028 0.40895155045087805 8 0.08470423135194438 0.36042417502098123 9 0.07026769227481655 0.3363698181468979 >10 0.3679867297057397 3.6442684177012485 >50 0.036596681921243095 1.3252745677942084 >100 0.027618279874629077 3.1071650002018223 >500 0.0034522849591087403 1.1474558411167368 >1k 0.002071370975465244 2.4259262033787437 >5k 0.002243985223420681 7.8543526765639085 >10k+ 0.001208299735688059 18.301085184282577 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 59983 5.416928182782833 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 51454 4.646693608470864 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 31495 2.8442417537759916 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 16200 1.4629851218025423 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 15364 1.3874878648996456 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 14520 1.3112681462082043 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 10318 0.931795091775224 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8091 0.7306797913891586 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 7606 0.6868805454586503 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7451 0.6728828483056014 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7087 0.6400108369268281 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7017 0.6336892962770642 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 6859 0.6194206759533109 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6668 0.6021719007518118 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6644 0.6000045153861784 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 5944 0.5367891088885377 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5915 0.5341701849050641 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5674 0.512406023525162 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5382 0.48603616824328905 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 5211 0.4705935475131511 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 4772 0.4309484568667735 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3293 0.2973833337096155 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3023 0.2730002483462398 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2648 0.23913485200821802 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2355 0.21267468900277695 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2104 0.19000745038719435 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1895 0.17113313616147022 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 1771 0.159934978439031 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 1372 0.1239021967353758 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.8061544713611632E-4 0.0 0.0 0.0 0.0 8 1.8061544713611632E-4 0.0 0.0 0.0 0.0 9 1.8061544713611632E-4 0.0 0.0 9.030772356805816E-5 0.0 10-11 1.8061544713611632E-4 0.0 0.0 9.030772356805816E-5 0.0 12-13 1.8061544713611632E-4 0.0 0.0 4.966924796243198E-4 0.0 14-15 1.8061544713611632E-4 0.0 0.0 0.0015352313006569886 0.0 16-17 1.8061544713611632E-4 0.0 0.0 0.0037477705280744133 0.0 18-19 1.8061544713611632E-4 0.0 0.0 0.005147540243379315 0.0 20-21 1.8061544713611632E-4 0.0 0.0 0.007269771747228681 0.0 22-23 1.8061544713611632E-4 0.0 0.0 0.011740004063847561 0.0 24-25 1.8061544713611632E-4 0.0 0.0 0.016797236583658816 0.0 26-27 1.8061544713611632E-4 0.0 0.0 0.021628699794549927 0.0 28-29 4.966924796243198E-4 0.0 0.0 0.0293500101596189 0.0 30-31 5.41846341408349E-4 0.0 0.0 0.04384439979229224 0.0 32-33 5.41846341408349E-4 0.0 0.0 0.07012394735059715 0.0 34-35 6.321540649764071E-4 0.0 0.0 0.10963357641162261 0.0 36-37 8.127695121125235E-4 0.0 0.0 0.16440521075564987 0.0 38-39 8.127695121125235E-4 0.0 0.0 0.2519133948930982 0.0 40-41 8.127695121125235E-4 0.0 0.0 0.38764590341588967 0.0 42-43 8.127695121125235E-4 0.0 0.0 0.48752624568216196 0.0 44-45 9.030772356805816E-4 0.0 0.0 0.5815817397782945 0.0 46-47 0.0010385388210326688 0.0 0.0 0.706522475334703 0.0 48-49 0.001174000406384756 0.0 0.0 0.8234709773553384 0.0 50-51 0.001174000406384756 0.0 0.0 0.9461088659607613 0.0 52-53 0.001174000406384756 0.0 0.0 1.1065856907412006 0.0 54-55 0.001174000406384756 0.0 0.0 1.2658433612534714 0.0 56-57 0.0013546158535208725 0.0 0.0 1.4334996500575712 0.0 58-59 0.0013546158535208725 0.0 0.0 1.6361953356060777 0.0 60-61 0.0013546158535208725 0.0 0.0 1.82281624635947 0.0 62-63 0.0013546158535208725 0.0 0.0 2.009211387803942 0.0 64-65 0.0013546158535208725 0.0 0.0 2.2268981554647462 0.0 66-67 0.0013546158535208725 0.0 0.0 2.4752443952769063 0.0 68-69 0.0014449235770889305 0.0 0.0 2.7835098096764725 0.0 70-71 0.0014449235770889305 0.0 0.0 3.2459305082067145 0.0 72-73 0.0014900774388729595 0.0 0.0 3.6053100941458016 0.0 74-75 0.0015352313006569886 0.0 0.0 3.938274670941232 0.0 76-77 0.0015352313006569886 0.0 0.0 4.331022960738718 0.0 78-79 0.0015352313006569886 0.0 0.0 4.69853024179893 0.0 80-81 0.001625539024225047 0.0 0.0 5.090239992775382 0.0 82-83 0.001625539024225047 0.0 0.0 5.583681394351252 0.0 84-85 0.001670692886009076 0.0 0.0 6.054049172555483 0.0 86-87 0.001715846747793105 0.0 0.0 6.519314564378119 0.0 88-89 0.001715846747793105 0.0 0.0 7.0673921387126635 0.0 90-91 0.001715846747793105 0.0 0.0 7.579346623619985 0.0 92-93 0.001715846747793105 0.0 0.0 8.090081954259139 0.0 94-95 0.001715846747793105 0.0 0.0 8.654414918835933 0.0 96-97 0.001715846747793105 0.0 0.0 9.211839342559774 0.0 98-99 0.001761000609577134 0.0 0.0 9.864944799403968 0.0 100-101 0.0018061544713611631 0.0 0.0 10.840990675727541 0.0 102-103 0.0018061544713611631 0.0 0.0 11.61393448174655 0.0 104-105 0.0018061544713611631 0.0 0.0 12.315083647528954 0.0 106-107 0.0020770776420653376 0.0 0.0 13.072042986476418 0.0 108-109 0.0020770776420653376 0.0 0.0 13.730837829905402 0.0 110-111 0.0022576930892014542 0.0 0.0 14.42697491702978 0.0 112-113 0.0024383085363375704 0.0 0.0 15.302508297022102 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1260 0.0 56.658283 5 ATAACGC 180 0.0 52.88106 3 TATAACG 165 0.0 50.477383 2 AAGTACT 1475 0.0 47.99629 4 AAAGTAC 1500 0.0 46.40313 3 AAAAGTA 1685 0.0 41.308426 2 TAACGCA 250 0.0 40.454014 4 CGATCCG 30 0.0013097272 39.660797 70-71 AAAAAGT 1835 0.0 37.00255 1 CTGTACG 65 0.0019402782 36.609966 4 GTTATCA 540 0.0 35.295483 1 GGCGTGA 75 0.003908936 31.735804 8 TCGACCG 40 0.005367146 29.745598 18-19 GTCGGGA 320 0.0 29.745598 2 CGGGAGT 355 0.0 26.812933 4 GTTCGAC 45 0.009536661 26.441128 16-17 TTATCAA 735 0.0 25.900928 2 ACAACGC 230 8.290954E-9 25.865738 3 GTGCGTT 115 0.0010056575 25.865738 4 GTATAAC 415 0.0 25.833738 1 >>END_MODULE