FastQCFastQC Report
Thu 26 May 2016
SRR1364161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986487
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA606892.0321200125766494No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT472731.5828965604069263No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG305881.0242133985515423No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC188110.6298704799317727No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG147350.493389055435366No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT145170.4860895091791794No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC142720.47788589068025406No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG116450.3899230098774915No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN109740.36745514043757765No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT103860.3477664560401569No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC71800.24041624825421976No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT70220.23512575142634137No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA60650.2030814130448249No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG60630.20301444473054797No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC55860.18704250177549744No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG55290.18513390481860462No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG46170.1545963535083193No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG40100.13427147012526758No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA38410.128612647568866No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC31510.10550857914332122No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG96500.029.4862444
ATGGGGA51250.026.134985
ATGGGAG45300.023.6541775
AGTACTC15200.023.4985565
ATGGGGG43050.023.2310945
CATGGGT56950.022.0550294
GTATAGG10100.021.8027631
ATGGGAT44950.020.1301695
ATGGGTA29450.019.4052585
GCGCGAC2158.537401E-519.3811049
TGGGGAG32750.017.9946236
GGCGCGA2652.077397E-517.9706178
CTGGTCG7500.017.461459
TGGGGGG24650.017.3873946
AAGTACT22650.017.3458124
TCGCGGA5700.017.2212682-83
GTCTAGA9000.017.193411
CGTTTAA4201.4708348E-817.0044731
TGGGATA19200.016.4321176
TAGGACA10550.016.3630564
Produced by FastQC (version 0.11.5)