##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364161_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2986487 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.682939855422106 31.0 31.0 34.0 27.0 34.0 2 30.781758969652305 31.0 31.0 34.0 27.0 34.0 3 30.894044742200453 33.0 31.0 34.0 27.0 34.0 4 34.33437078413534 37.0 35.0 37.0 30.0 37.0 5 34.33026462194545 37.0 35.0 37.0 31.0 37.0 6 34.275375047673066 37.0 35.0 37.0 30.0 37.0 7 34.2132806873092 37.0 35.0 37.0 30.0 37.0 8 34.15624477856424 37.0 35.0 37.0 30.0 37.0 9 35.59615628663376 39.0 37.0 39.0 30.0 39.0 10-11 35.50741573628146 39.0 37.0 39.0 29.0 39.0 12-13 35.41423317764316 39.0 37.0 39.0 28.5 39.0 14-15 36.64256650037318 40.0 37.0 41.0 28.0 41.0 16-17 36.682558303451515 40.0 37.0 41.0 29.0 41.0 18-19 36.6811928195234 40.0 37.0 41.0 29.0 41.0 20-21 36.69318299393234 40.0 37.0 41.0 29.0 41.0 22-23 36.65806012214351 40.0 37.0 41.0 29.0 41.0 24-25 36.63041325811899 40.0 37.0 41.0 29.5 41.0 26-27 36.51198967214657 40.0 37.0 41.0 29.0 41.0 28-29 36.43706753118296 40.0 36.0 41.0 29.0 41.0 30-31 36.524465031992435 40.0 37.0 41.0 30.0 41.0 32-33 36.33787674280852 40.0 36.0 41.0 29.5 41.0 34-35 36.41227217798035 40.0 36.0 41.0 30.0 41.0 36-37 36.39571191838438 40.0 36.0 41.0 30.0 41.0 38-39 36.31848372351864 40.0 36.0 41.0 29.5 41.0 40-41 36.164360835992255 40.0 36.0 41.0 29.0 41.0 42-43 36.031936686816316 40.0 35.0 41.0 28.5 41.0 44-45 35.87947461348401 40.0 35.0 41.0 28.0 41.0 46-47 35.70637558442411 40.0 35.0 41.0 27.5 41.0 48-49 35.55511827106564 39.0 35.0 41.0 27.0 41.0 50-51 35.44611478302099 39.0 35.0 41.0 27.0 41.0 52-53 35.297345007696336 39.0 35.0 41.0 26.5 41.0 54-55 35.095605472248835 39.0 34.0 41.0 26.0 41.0 56-57 34.85513464481848 39.0 34.0 41.0 26.0 41.0 58-59 34.608145289097195 38.0 34.0 40.5 25.0 41.0 60-61 34.37495040159224 38.0 34.0 40.0 25.0 41.0 62-63 34.0128728167911 37.5 33.0 40.0 23.5 41.0 64-65 33.77438207499313 37.0 33.0 40.0 23.0 41.0 66-67 33.45151443820114 36.5 33.0 39.5 22.5 41.0 68-69 33.094228436286514 36.0 33.0 39.0 22.0 41.0 70-71 32.78469201439685 35.5 32.0 39.0 22.0 41.0 72-73 32.60453000465095 35.0 32.0 39.0 21.5 40.5 74-75 32.321410238852536 35.0 32.5 37.5 20.5 39.5 76-77 31.932848862225082 35.0 32.0 37.0 20.0 39.0 78-79 31.584734170950686 35.0 32.0 36.5 19.0 39.0 80-81 31.30354727812309 35.0 32.0 36.0 18.0 38.0 82-83 31.036938885051235 35.0 32.0 36.0 15.5 37.0 84-85 30.780645454006667 35.0 32.0 35.0 11.5 37.0 86-87 30.496893172479908 35.0 31.0 35.0 7.0 36.0 88-89 30.282681625602255 35.0 31.0 35.0 6.5 36.0 90-91 30.134664239288504 35.0 31.0 35.0 2.0 36.0 92-93 29.92233801787853 35.0 31.0 35.0 2.0 36.0 94-95 29.84620241106022 35.0 31.0 35.0 2.0 35.0 96-97 29.76896768678384 35.0 31.0 35.0 2.0 35.0 98-99 29.620492404621217 35.0 31.0 35.0 2.0 35.0 100-101 29.379985246880366 35.0 30.0 35.0 2.0 35.0 102-103 28.27419657276258 33.5 28.0 34.5 2.0 35.0 104-105 29.048161602578546 34.5 30.0 35.0 2.0 35.0 106-107 29.12414016869988 35.0 30.0 35.0 2.0 35.0 108-109 29.07377731763105 35.0 30.0 35.0 2.0 35.0 110-111 29.044753585064996 35.0 30.0 35.0 2.0 35.0 112-113 28.9473187058909 35.0 30.0 35.0 2.0 35.0 114-115 28.81417766091063 35.0 30.0 35.0 2.0 35.0 116-117 28.60688678705114 35.0 30.0 35.0 2.0 35.0 118-119 28.43884487024387 35.0 30.0 35.0 2.0 35.0 120-121 28.264357420608228 35.0 30.0 35.0 2.0 35.0 122-123 27.974997212443917 35.0 29.0 35.0 2.0 35.0 124-125 27.040649431924532 34.0 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39014871235205106 1101 2 0.42316922795168566 1101 3 0.47214971940246997 1101 4 0.4464300915127595 1101 5 0.36293463999091813 1101 6 0.36796664208104346 1101 7 0.4261263517692484 1101 8 0.4218161116044996 1101 9 0.6120992772239404 1101 10-11 0.6730011356021208 1101 12-13 0.6601088681981508 1101 14-15 0.6744226657282937 1101 16-17 0.6146314411858569 1101 18-19 0.6140221855924182 1101 20-21 0.660507253509131 1101 22-23 0.6069097435845237 1101 24-25 0.6492819851348628 1101 26-27 0.7824125902836698 1101 28-29 0.8467072285945179 1101 30-31 0.7408134196512108 1101 32-33 0.8253069889316507 1101 34-35 0.6969327705888517 1101 36-37 0.6642948703519096 1101 38-39 0.6288728092194091 1101 40-41 0.8002232280335733 1101 42-43 0.7740237611880403 1101 44-45 0.7387378112284395 1101 46-47 0.8561201818614919 1101 48-49 0.8165186381370546 1101 50-51 0.7351380570883421 1101 52-53 0.7140260775137364 1101 54-55 0.7584351763938386 1101 56-57 0.7350734583221836 1101 58-59 0.7202851581382461 1101 60-61 0.7103041283316216 1101 62-63 0.7197939622046974 1101 64-65 0.6791045815605798 1101 66-67 0.6790733943263874 1101 68-69 0.7428073822349006 1101 70-71 0.7279613351913952 1101 72-73 0.6240365577264342 1101 74-75 0.5884642687631398 1101 76-77 0.6125717203822383 1101 78-79 0.5379555446363646 1101 80-81 0.45276743300309263 1101 82-83 0.5421246826011945 1101 84-85 0.5708333918112096 1101 86-87 0.5478999012778054 1101 88-89 0.5144719272863121 1101 90-91 0.48168313881931724 1101 92-93 0.545906395323918 1101 94-95 0.512498200461895 1101 96-97 0.45368305490802996 1101 98-99 0.5384494782393041 1101 100-101 0.5483147119381897 1101 102-103 0.573031553833264 1101 104-105 0.5458630186467595 1101 106-107 0.5061848158439055 1101 108-109 0.5391312237136177 1101 110-111 0.4766133467088558 1101 112-113 0.42680014088116636 1101 114-115 0.5102776656624748 1101 116-117 0.5139924768788795 1101 118-119 0.5216156501255611 1101 120-121 0.48169342098691104 1101 122-123 0.5995756016316776 1101 124-125 0.6447991632281713 1102 1 0.22379110249188727 1102 2 0.3078016412221416 1102 3 0.3127971510990193 1102 4 0.32547169536230314 1102 5 0.23508788618937615 1102 6 0.3388380578406682 1102 7 0.3035193011402981 1102 8 0.37742640085773616 1102 9 0.4601335435908922 1102 10-11 0.4180611961775753 1102 12-13 0.4486295182176647 1102 14-15 0.5513095718168373 1102 16-17 0.47042144368985106 1102 18-19 0.5309764098055325 1102 20-21 0.4662811717720885 1102 22-23 0.48809389172004103 1102 24-25 0.529391565716594 1102 26-27 0.6279005322701039 1102 28-29 0.6745819380249642 1102 30-31 0.5624361548010484 1102 32-33 0.6977514439917059 1102 34-35 0.5883749789169173 1102 36-37 0.50671275491014 1102 38-39 0.5119873562752204 1102 40-41 0.5280633962861785 1102 42-43 0.5720299480186455 1102 44-45 0.534842282394365 1102 46-47 0.49565066965769233 1102 48-49 0.45298747839341047 1102 50-51 0.44174829261319815 1102 52-53 0.45041074506926293 1102 54-55 0.4903446760824579 1102 56-57 0.3967873861887483 1102 58-59 0.4169551956147117 1102 60-61 0.3486554390535943 1102 62-63 0.5339241459056652 1102 64-65 0.4842358156275992 1102 66-67 0.44637310149882126 1102 68-69 0.3549742023622571 1102 70-71 0.40139118662531814 1102 72-73 0.44731374727778217 1102 74-75 0.41723061090302593 1102 76-77 0.4302209019155576 1102 78-79 0.37908592537653263 1102 80-81 0.4122181676397929 1102 82-83 0.4587258496827893 1102 84-85 0.4862502409777285 1102 86-87 0.3879370228941248 1102 88-89 0.41198536320341006 1102 90-91 0.4419972447224332 1102 92-93 0.4872975669658359 1102 94-95 0.5230616266008532 1102 96-97 0.5051278974556759 1102 98-99 0.5647644143750341 1102 100-101 0.5498665867923016 1102 102-103 0.4951965870570696 1102 104-105 0.5545345362635743 1102 106-107 0.5653520449712879 1102 108-109 0.46846411815095834 1102 110-111 0.480988334087435 1102 112-113 0.5679547652995076 1102 114-115 0.6180875308991567 1102 116-117 0.550329732700142 1102 118-119 0.6152355028191181 1102 120-121 0.6426728676942943 1102 122-123 0.6133000385369272 1102 124-125 0.5924159195010112 1103 1 -0.04915983388332279 1103 2 -0.0278578185960221 1103 3 0.018623880378665802 1103 4 -0.012904573017380017 1103 5 -0.1070552656637247 1103 6 -0.033676783041542535 1103 7 -0.049995222689176444 1103 8 -0.025491803171981076 1103 9 0.07506718423586989 1103 10-11 0.07523001497077075 1103 12-13 0.06148832237119706 1103 14-15 0.02139248427383933 1103 16-17 0.022006276377290135 1103 18-19 -0.026110139359502682 1103 20-21 0.03839062547869787 1103 22-23 0.08136181228717021 1103 24-25 0.060307088973544865 1103 26-27 0.12812058950574823 1103 28-29 0.11276774110354637 1103 30-31 -0.01901005013589696 1103 32-33 0.0714022693413412 1103 34-35 -0.033533562221997215 1103 36-37 0.037911631714749205 1103 38-39 0.12325388691971995 1103 40-41 0.15014796244370388 1103 42-43 0.10349421239555312 1103 44-45 0.17952358001133462 1103 46-47 0.11031531395786232 1103 48-49 0.06613106402615188 1103 50-51 0.04404390809479963 1103 52-53 0.09573500132721335 1103 54-55 0.08496661328607757 1103 56-57 0.08001541086714781 1103 58-59 0.1458816257764468 1103 60-61 0.07459536332440564 1103 62-63 0.11671522871707651 1103 64-65 0.11702529915565663 1103 66-67 0.0632660459902894 1103 68-69 0.06701612061748818 1103 70-71 0.08100013172867193 1103 72-73 0.06373163191815223 1103 74-75 -0.01418677058618556 1103 76-77 -0.06578743222001648 1103 78-79 -0.011703869284829693 1103 80-81 0.00693266077133714 1103 82-83 0.026712942843019505 1103 84-85 -0.008165897535995725 1103 86-87 -0.01862336723963054 1103 88-89 0.02129921041141003 1103 90-91 0.07555518909499526 1103 92-93 -0.04971963768832666 1103 94-95 -0.001984370969278615 1103 96-97 0.022105250876421678 1103 98-99 0.06205020205272049 1103 100-101 0.07468484225870498 1103 102-103 0.0828866174881071 1103 104-105 0.08431565875902436 1103 106-107 0.0988392729801788 1103 108-109 0.1569864457562602 1103 110-111 0.1920173815386903 1103 112-113 0.11977647885269604 1103 114-115 0.12327608900293541 1103 116-117 0.08983805484108842 1103 118-119 0.05109767180251268 1103 120-121 0.0983309452747001 1103 122-123 0.10414889244789549 1103 124-125 0.19697021117270452 1104 1 0.2533117734017303 1104 2 0.3141138083632349 1104 3 0.3136053967688994 1104 4 0.33738914052702995 1104 5 0.32703422640513935 1104 6 0.3536054468529386 1104 7 0.2539761876510127 1104 8 0.27470682515068034 1104 9 0.42867529114345615 1104 10-11 0.4247541020775998 1104 12-13 0.41048556676577164 1104 14-15 0.5368001743317095 1104 16-17 0.5136181189495872 1104 18-19 0.42305192212380405 1104 20-21 0.4927875969494764 1104 22-23 0.43476846143770587 1104 24-25 0.5350084974436768 1104 26-27 0.5086888961085378 1104 28-29 0.5407436773218066 1104 30-31 0.4376470714997609 1104 32-33 0.5158740108977611 1104 34-35 0.5026093183328726 1104 36-37 0.5019957650985418 1104 38-39 0.521196695430497 1104 40-41 0.5355955221731605 1104 42-43 0.524754399880571 1104 44-45 0.4845512618182113 1104 46-47 0.5040593533841573 1104 48-49 0.48058620826240883 1104 50-51 0.43820572010109515 1104 52-53 0.49417661173986005 1104 54-55 0.5568578052061071 1104 56-57 0.5939294840884486 1104 58-59 0.49569727646320416 1104 60-61 0.4676174406200957 1104 62-63 0.5087526831266658 1104 64-65 0.5047254030435582 1104 66-67 0.4977785841369524 1104 68-69 0.500030072670036 1104 70-71 0.40453484980139365 1104 72-73 0.3426023083091465 1104 74-75 0.25023442043734434 1104 76-77 0.2612668892694465 1104 78-79 0.3035811206772685 1104 80-81 0.21671686518509503 1104 82-83 0.28807200638339125 1104 84-85 0.31752983908443255 1104 86-87 0.3194796420210402 1104 88-89 0.3032745572148805 1104 90-91 0.307249458466071 1104 92-93 0.2591265635881044 1104 94-95 0.24947851760130746 1104 96-97 0.23846271816958264 1104 98-99 0.2635185568065985 1104 100-101 0.2596627665856346 1104 102-103 0.13317398255248492 1104 104-105 0.2967478689590237 1104 106-107 0.3371648644131007 1104 108-109 0.3693551204491925 1104 110-111 0.2472711645634611 1104 112-113 0.2651128103723508 1104 114-115 0.22556795114396877 1104 116-117 0.18152298606453598 1104 118-119 0.19214036198411222 1104 120-121 0.1884477715052384 1104 122-123 0.11397925010868803 1104 124-125 0.04036020621155956 1105 1 0.29778732761199933 1105 2 0.42733473814885414 1105 3 0.386812441269079 1105 4 0.32178260819689797 1105 5 0.24529722043782698 1105 6 0.2639600741603516 1105 7 0.2991772360140885 1105 8 0.26470905928830035 1105 9 0.42453432907095134 1105 10-11 0.38239287085156093 1105 12-13 0.49473357526029815 1105 14-15 0.5375469888838751 1105 16-17 0.5115971853202907 1105 18-19 0.4597803638968898 1105 20-21 0.47710058761895624 1105 22-23 0.4537958668592097 1105 24-25 0.5299444521651537 1105 26-27 0.6777032090031128 1105 28-29 0.6961874058812896 1105 30-31 0.48883314633616237 1105 32-33 0.39384024899437264 1105 34-35 0.49171279253698685 1105 36-37 0.49238536987238746 1105 38-39 0.5160410625209266 1105 40-41 0.5273889701441803 1105 42-43 0.5689080460892555 1105 44-45 0.5616620414121343 1105 46-47 0.6635446489149146 1105 48-49 0.6438572259721482 1105 50-51 0.5804608297900486 1105 52-53 0.5850195302442813 1105 54-55 0.5993538654365338 1105 56-57 0.5653367265380211 1105 58-59 0.5618376595817764 1105 60-61 0.5452804485861193 1105 62-63 0.5823708209609535 1105 64-65 0.5668494433253031 1105 66-67 0.5549580934914928 1105 68-69 0.588399524561602 1105 70-71 0.5696688500127962 1105 72-73 0.5312881429156988 1105 74-75 0.5107327652536711 1105 76-77 0.4841034993379587 1105 78-79 0.4171635963900293 1105 80-81 0.3971901420163668 1105 82-83 0.41515029488983757 1105 84-85 0.4689725394601503 1105 86-87 0.4537948548166426 1105 88-89 0.40932102247284163 1105 90-91 0.3429280957862595 1105 92-93 0.3488304923071013 1105 94-95 0.3757030892714823 1105 96-97 0.33399762596936355 1105 98-99 0.3313819885380802 1105 100-101 0.3596974797992374 1105 102-103 0.1775966861953293 1105 104-105 0.323304077939774 1105 106-107 0.33705045494612307 1105 108-109 0.4638432266692263 1105 110-111 0.40586250475776 1105 112-113 0.42773370604533056 1105 114-115 0.4951680044749942 1105 116-117 0.3703556611680767 1105 118-119 0.4351184434124242 1105 120-121 0.44819029314994907 1105 122-123 0.4795682456596282 1105 124-125 0.48391910336435373 1106 1 0.09397039971452514 1106 2 0.22983735712197984 1106 3 0.22650477269620595 1106 4 0.24507561012819679 1106 5 0.17535387729510887 1106 6 0.1532264205908973 1106 7 0.1774637434409172 1106 8 0.20305679501352358 1106 9 0.2486107391801866 1106 10-11 0.30623610707901605 1106 12-13 0.32837560828915713 1106 14-15 0.3000957858813038 1106 16-17 0.3040093085782587 1106 18-19 0.36977587772922504 1106 20-21 0.3819629582287476 1106 22-23 0.4099627644503059 1106 24-25 0.5167513750363923 1106 26-27 0.5741543337893376 1106 28-29 0.5855537770901549 1106 30-31 0.4936616084971064 1106 32-33 0.2218387477895689 1106 34-35 0.4642333003119674 1106 36-37 0.3994211353221715 1106 38-39 0.2935641716262509 1106 40-41 0.34434879615497493 1106 42-43 0.2947777666824649 1106 44-45 0.28516674693840116 1106 46-47 0.36890431567018567 1106 48-49 0.4168880169412432 1106 50-51 0.3297402691259279 1106 52-53 0.29939492867713113 1106 54-55 0.3803240557982477 1106 56-57 0.3232213352877409 1106 58-59 0.46159982016403234 1106 60-61 0.4318242440505813 1106 62-63 0.5096281317381823 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0.4427338791778581 2207 66-67 0.3934441573151446 2207 68-69 0.35602151820103956 2207 70-71 0.43916947837501397 2207 72-73 0.4378463129069132 2207 74-75 0.3506268333219609 2207 76-77 0.41129242798038135 2207 78-79 0.3841216820652953 2207 80-81 0.40504667345691914 2207 82-83 0.440895312875103 2207 84-85 0.4529308687860727 2207 86-87 0.5560317521820082 2207 88-89 0.5667950865016671 2207 90-91 0.5606982835255003 2207 92-93 0.5519067020646702 2207 94-95 0.6127363059089781 2207 96-97 0.6164045658453574 2207 98-99 0.6179790785156989 2207 100-101 0.5546504648403712 2207 102-103 0.6049467128045443 2207 104-105 0.5230483248989515 2207 106-107 0.5658089666894455 2207 108-109 0.5590759691497169 2207 110-111 0.5059512288618606 2207 112-113 0.5354571513297302 2207 114-115 0.5943809086625826 2207 116-117 0.6691831725330957 2207 118-119 0.5336470819823198 2207 120-121 0.5697082527680593 2207 122-123 0.6241528693796639 2207 124-125 0.6479218428312734 2208 1 0.17939406788295642 2208 2 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-1.7376439760771447 2216 84-85 -1.8041040260028822 2216 86-87 -1.81837541682955 2216 88-89 -1.8679184793845351 2216 90-91 -1.8843377357746007 2216 92-93 -1.9689047389824559 2216 94-95 -1.8987143150545585 2216 96-97 -1.8544384862663712 2216 98-99 -1.8397889423604887 2216 100-101 -1.9076065090419405 2216 102-103 -1.8419716017062981 2216 104-105 -1.9309333055030748 2216 106-107 -1.923387476648763 2216 108-109 -1.9663025335238444 2216 110-111 -1.9639569310539962 2216 112-113 -1.9402634204569935 2216 114-115 -2.0073097160577547 2216 116-117 -2.0238249872878917 2216 118-119 -2.135684422070316 2216 120-121 -2.189151047517843 2216 122-123 -2.224374929383437 2216 124-125 -2.3126082229791933 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72963.0 3 13440.0 4 8511.0 5 6671.0 6 7655.0 7 7550.0 8 7859.0 9 8261.0 10 9704.0 11 11230.0 12 12151.0 13 13212.0 14 14143.0 15 13417.0 16 12659.0 17 12577.0 18 12632.0 19 12783.0 20 13140.0 21 14981.0 22 17646.0 23 22705.0 24 27287.0 25 30965.0 26 35624.0 27 39271.0 28 45668.0 29 56526.0 30 67688.0 31 81837.0 32 104101.0 33 135414.0 34 180537.0 35 250681.0 36 425357.0 37 794297.0 38 383717.0 39 1627.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.0948984312571 18.51442991125878 11.737090465575976 23.653581191908145 2 14.842222974305008 21.52787262917302 38.21279561955885 25.417108776963122 3 20.44586944946287 27.09455611680451 28.332121847347384 24.12745258638523 4 13.095902833746626 17.186509799911466 34.8847785517363 34.83280881460561 5 12.77478136905791 39.57796945524415 33.69489824133196 13.95235093436598 6 32.10673179297503 33.27194102301455 18.712207987055475 15.90911919695494 7 27.617411695695576 33.09726321907432 21.16168560519426 18.12363948003584 8 27.639018183396374 34.07697066978162 20.92094846899416 17.36306267782784 9 28.693729266552015 15.908213768136667 19.784222407585055 35.61383455772626 10-11 24.72372037493674 28.042087510364063 26.0473758735423 21.186816241156897 12-13 25.755359492299355 25.748124892742858 26.166402519659606 22.33011309529818 14-15 24.903862230396253 25.92650031605786 24.133548995023478 25.03608845852241 16-17 22.983118770128442 29.405918909944685 26.878595701289804 20.732366618637073 18-19 23.571292100362744 28.255764749254595 27.770867194816102 20.40207595556656 20-21 23.05243938643403 28.106562471353158 27.316620257028934 21.524377885183874 22-23 22.3045075720616 28.728697793823244 25.150213148234755 23.816581485880402 24-25 22.48784012821608 29.367461266376043 24.85798063705684 23.286717968351027 26-27 23.222847279933518 27.733188209288162 25.222547835419913 23.82141667535841 28-29 22.875336964064175 27.465062977059105 26.885648800386903 22.773951258489813 30-31 23.891009380955207 28.251162419784748 26.077790308000655 21.780037891259386 32-33 21.485776754449276 28.549199680619218 26.994052155433323 22.97097140949818 34-35 21.046972960569658 29.022656112790013 27.210769341540107 22.719601585100218 36-37 23.622289017341043 28.03094062007357 25.949948502364688 22.396821860220705 38-39 24.263161778561166 27.297922829012318 26.017830257042196 22.421085135384324 40-41 22.382182650822493 28.765664900755976 26.02548068106927 22.82667176735226 42-43 23.78020409384525 27.98779901523408 25.65057686734707 22.5814200235736 44-45 24.40353310652102 27.253175980538956 25.670024235500527 22.673266677439493 46-47 22.77060059623746 28.253005402729347 27.60726085927074 21.369133141762454 48-49 23.687287826067653 27.692351413506355 27.306074623341875 21.314286137084117 50-51 21.814749098666567 28.278221534584585 27.396433875509302 22.510595491239542 52-53 21.57468757933459 28.94230074093253 25.36978535317448 24.113226326558404 54-55 22.002827609153734 29.078609316651033 24.95453346382458 23.96402961037066 56-57 22.978939143929523 27.74237601313597 25.257377216728727 24.02130762620578 58-59 22.99520671574209 27.06646397236561 26.59725569220367 23.341073619688625 60-61 23.655229367484615 28.125043081954402 26.178041367418707 22.04168618314227 62-63 21.24235784807224 28.438203395734853 27.00424281918489 23.315195937008024 64-65 20.52288441124597 28.57460417441901 27.401062731634955 23.501448682700065 66-67 22.872947665145976 28.144548398063264 26.02949222040379 22.95301171638697 68-69 23.62308318800949 27.20936519988782 26.039808085094652 23.12774352700804 70-71 21.865278770802803 28.468467317164382 26.134262513292335 23.53199139874048 72-73 23.239659243430555 27.83320066144252 25.8634350551757 23.06370503995122 74-75 23.77991503942148 27.100329288664515 25.82561792492649 23.294137746987516 76-77 22.186521506799238 27.789131236756553 27.90863120329589 22.11571605314831 78-79 23.067586736561314 27.50098397577999 27.491787311932846 21.939641975725852 80-81 21.32702752600246 28.239331921157117 27.520453743718605 22.913186809121818 82-83 21.1321085920598 28.63556428462498 25.56183482857226 24.67049229474296 84-85 21.500646482513027 28.675089154057876 25.26098894170224 24.563275421726857 86-87 22.297021105873732 26.948818159515888 25.86481351666421 24.889347217946174 88-89 22.45604336759613 26.57763282906717 27.03268794056879 23.933635862767915 90-91 22.921307849240826 27.819134859469052 26.47869127646546 22.780866014824657 92-93 21.026152627983226 27.67682613806633 27.274514231829922 24.02250700212052 94-95 20.21915808925219 27.997807410247162 27.72301067458286 24.06002382591779 96-97 22.579437599679412 27.321454505845615 26.555099686135918 23.544008208339047 98-99 23.158965468797156 26.612052143319513 26.36287036968573 23.866112018197597 100-101 21.555177373518468 27.624001985651752 26.444508713357827 24.376311927471953 102-103 22.73383683460553 26.949779301651986 26.448383838876012 23.868000024866472 104-105 23.147119642169155 26.08442817180276 26.683742075082172 24.08471011094591 106-107 21.68656354984214 26.968941099523292 28.403133332672336 22.94136201796223 108-109 22.482973441190055 27.002213066098207 27.580420280881917 22.93439321182982 110-111 20.872154279263377 27.599330857668075 27.882280839413237 23.64623402365531 112-113 20.59427398868823 27.89287012740998 26.199650820581716 25.313205063320076 114-115 20.773783380791766 28.533501685963515 25.68594536237752 25.006769570867192 116-117 21.537130938953947 27.047370479955802 26.097957872294092 25.317540708796166 118-119 21.40542302128803 26.89725532822957 26.991593015446156 24.705728635036245 120-121 21.92254913831376 27.754839838021823 26.6269625055495 23.69564851811492 122-123 20.35538178496057 27.897133933816825 27.23355401115632 24.513930270066286 124-125 19.919599655566365 28.236918976239423 27.442983643772383 24.400497724421825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12566.0 1 7228.0 2 2567.0 3 3511.5 4 3543.0 5 2991.0 6 2381.5 7 2036.0 8 1968.5 9 1902.0 10 1750.5 11 1619.5 12 1615.5 13 1663.0 14 1726.5 15 1855.5 16 2044.0 17 2238.0 18 2517.5 19 2905.5 20 3535.5 21 4493.5 22 5545.0 23 7072.5 24 8832.0 25 10843.5 26 14117.5 27 17481.5 28 19647.0 29 23035.0 30 28327.5 31 33204.0 32 37932.0 33 43930.0 34 51297.0 35 56384.5 36 61622.5 37 67397.0 38 69787.5 39 71210.5 40 72470.5 41 77780.5 42 83936.0 43 87885.5 44 92979.0 45 99408.0 46 104639.5 47 108487.5 48 115487.5 49 143103.0 50 235185.5 51 235575.0 52 133740.5 53 94110.5 54 83840.5 55 74935.5 56 64863.5 57 57398.5 58 53251.0 59 48495.5 60 42837.0 61 36993.0 62 31825.0 63 26144.5 64 20882.0 65 16737.5 66 12173.0 67 9436.5 68 7201.5 69 4735.0 70 3775.0 71 3401.0 72 3029.5 73 2808.5 74 2180.0 75 1715.0 76 1188.0 77 594.0 78 383.0 79 264.5 80 158.5 81 96.5 82 67.5 83 41.0 84 30.5 85 32.5 86 25.5 87 17.5 88 15.0 89 12.0 90 10.5 91 9.0 92 9.5 93 9.5 94 8.0 95 7.0 96 5.0 97 2.5 98 1.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48089946482271645 2 0.46881168409572854 3 0.4538442658548321 4 0.45548498955461714 5 0.45769494392575627 6 0.46214833682517287 7 0.44731485521283026 8 0.47413566508074534 9 0.4867926764790873 10-11 0.4915139426356117 12-13 0.49077729117856533 14-15 0.4664343089388971 16-17 0.46924697813852867 18-19 0.45508317966895556 20-21 0.4642410966463273 22-23 0.4814017271797935 24-25 0.4689456207242824 26-27 0.47939267775148525 28-29 0.4746211853592532 30-31 0.48757955417184135 32-33 0.48505150030788685 34-35 0.4791248044943775 36-37 0.4369515085784736 38-39 0.39968364168335574 40-41 0.39255151621286144 42-43 0.4009058134189099 44-45 0.4338374819645959 46-47 0.44115377029935166 48-49 0.4386759426711049 50-51 0.4251985694228704 52-53 0.419623457259315 54-55 0.4217831853947464 56-57 0.4037854509328184 58-59 0.4092266264678198 60-61 0.4186859008594379 62-63 0.41925513153079186 64-65 0.4327325047790263 66-67 0.4225030947732235 68-69 0.4242107867872855 70-71 0.4342560339288267 72-73 0.4348252646001807 74-75 0.43891033177107414 76-77 0.4301374826007949 78-79 0.4213144071948078 80-81 0.42946779945802543 82-83 0.42760942873684027 84-85 0.4253157639728551 86-87 0.4313931384934875 88-89 0.42061123989489996 90-91 0.42384246105876233 92-93 0.4327325047790263 94-95 0.4297524147937024 96-97 0.4001691619618636 98-99 0.37331486793681 100-101 0.3730637367582715 102-103 0.3546809344892511 104-105 0.35719224627463636 106-107 0.37463749214377967 108-109 0.38269043193558183 110-111 0.3995664471333711 112-113 0.3950628279982468 114-115 0.39439314485547733 116-117 0.36708681470905447 118-119 0.43951304659956664 120-121 0.44356462961332155 122-123 0.3997840941547711 124-125 0.39338862014132325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2986487.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.12431326389356 #Duplication Level Percentage of deduplicated Percentage of total 1 82.34885815843884 39.62982246940648 2 10.429434970484868 10.038187913700407 3 3.177735468363162 4.58779011447883 4 1.335077483903248 2.5699874826772287 5 0.7103874649218946 1.7093454450322227 6 0.40483250913817626 1.168937189350419 7 0.27670659520988067 0.932142040904598 8 0.1810060695746063 0.6968634235899575 9 0.13533062401650447 0.5861424009931613 >10 0.8337839462818156 7.802551998027332 >50 0.10478708831761191 3.4914444675535026 >100 0.05653989721230787 4.948527248754997 >500 0.001790180801277623 0.6067199786792331 >1k 0.001342635600958217 1.751669714802344 >5k 8.950904006388115E-4 2.6882770798300655 >10k+ 0.001491817334398019 16.791591032219362 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 60689 2.0321200125766494 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 47273 1.5828965604069263 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 30588 1.0242133985515423 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 18811 0.6298704799317727 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 14735 0.493389055435366 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 14517 0.4860895091791794 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 14272 0.47788589068025406 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 11645 0.3899230098774915 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 10974 0.36745514043757765 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 10386 0.3477664560401569 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 7180 0.24041624825421976 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 7022 0.23512575142634137 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 6065 0.2030814130448249 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6063 0.20301444473054797 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 5586 0.18704250177549744 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 5529 0.18513390481860462 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 4617 0.1545963535083193 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 4010 0.13427147012526758 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 3841 0.128612647568866 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3151 0.10550857914332122 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.348415713847072E-5 0.0 3 0.0 0.0 0.0 3.348415713847072E-5 0.0 4 0.0 0.0 0.0 3.348415713847072E-5 0.0 5 0.0 0.0 0.0 3.348415713847072E-5 0.0 6 0.0 0.0 0.0 3.348415713847072E-5 0.0 7 0.0 0.0 0.0 3.348415713847072E-5 0.0 8 0.0 0.0 0.0 1.0045247141541215E-4 0.0 9 0.0 0.0 0.0 1.3393662855388288E-4 0.0 10-11 3.348415713847072E-5 0.0 0.0 2.009049428308243E-4 0.0 12-13 3.348415713847072E-5 0.0 0.0 6.194569070617083E-4 0.0 14-15 3.348415713847072E-5 0.0 0.0 8.87330164169474E-4 0.0 16-17 3.348415713847072E-5 0.0 0.0 0.0016239816212158297 0.0 18-19 3.348415713847072E-5 0.0 0.0 0.002193212292569832 0.0 20-21 3.348415713847072E-5 0.0 0.0 0.0031140266138777765 0.0 22-23 8.37103928461768E-5 0.0 0.0 0.005206786435032197 0.0 24-25 1.3393662855388288E-4 0.0 0.0 0.0074334828847404995 0.0 26-27 1.674207856923536E-4 0.0 0.0 0.009241627370217918 0.0 28-29 1.674207856923536E-4 0.0 0.0 0.01359456779821911 0.0 30-31 1.674207856923536E-4 0.0 0.0 0.02348913623263721 0.0 32-33 2.009049428308243E-4 0.0 0.0 0.04388098792996588 0.0 34-35 2.511311785385304E-4 0.0 0.0 0.07475338081163588 0.0 36-37 3.0135741424623647E-4 0.0 0.0 0.12392486556948012 0.0 38-39 3.348415713847072E-4 0.0 0.0 0.19705426475990018 0.0 40-41 3.348415713847072E-4 0.0 0.0 0.287026194990971 0.0 42-43 3.348415713847072E-4 0.0 0.0 0.3645922450022384 0.0 44-45 3.683257285231779E-4 0.0 0.0 0.4529234515335242 0.0 46-47 3.683257285231779E-4 0.0 0.0 0.5652125724973858 0.0 48-49 4.018098856616486E-4 0.0 0.0 0.6758274856043238 0.0 50-51 4.018098856616486E-4 0.0 0.0 0.793859139517433 0.0 52-53 4.018098856616486E-4 0.0 0.0 0.9246147731431611 0.0 54-55 4.018098856616486E-4 0.0 0.0 1.0641599980177379 0.0 56-57 4.018098856616486E-4 0.0 0.0 1.2304590644459528 0.0 58-59 4.018098856616486E-4 0.0 0.0 1.4162793944858958 0.0 60-61 4.018098856616486E-4 0.0 0.0 1.6051132986683014 0.0 62-63 4.1855196423088396E-4 0.0 0.0 1.78951055202986 0.0 64-65 4.352940428001193E-4 0.0 0.0 1.9955218288243008 0.0 66-67 4.352940428001193E-4 0.0 0.0 2.2337113806288125 0.0 68-69 5.022623570770608E-4 0.0 0.0 2.5171882549631057 0.0 70-71 5.357465142155315E-4 0.0 0.0 2.8711660221524484 0.0 72-73 5.357465142155315E-4 0.0 0.0 3.187624791268135 0.0 74-75 5.357465142155315E-4 0.0 0.0 3.4934355984137886 0.0 76-77 5.357465142155315E-4 0.0 0.0 3.842072642539546 0.0 78-79 5.357465142155315E-4 0.0 0.0 4.213947691719401 0.0 80-81 5.692306713540022E-4 0.0 0.0 4.617264364452281 0.0 82-83 5.859727499232376E-4 0.0 0.0 5.061917229172604 0.0 84-85 6.194569070617083E-4 0.0 0.0 5.4921719063233825 0.0 86-87 6.361989856309437E-4 0.0 0.0 5.938532463057767 0.0 88-89 6.361989856309437E-4 0.0 0.0 6.452179433561907 0.0 90-91 6.361989856309437E-4 0.0 0.0 6.9824010618495915 0.0 92-93 6.361989856309437E-4 0.0 0.0 7.52610006338551 0.0 94-95 6.361989856309437E-4 0.0 0.0 8.08575426579791 0.0 96-97 6.361989856309437E-4 0.0 0.0 8.656508466301712 0.0 98-99 7.031672999078851E-4 0.0 0.0 9.295202021639472 0.0 100-101 7.199093784771204E-4 0.0 0.0 10.087286500828565 0.0 102-103 7.701356141848265E-4 0.0 0.0 10.83272754912377 0.0 104-105 8.371039284617679E-4 0.0 0.0 11.53468941937467 0.0 106-107 8.705880856002386E-4 0.0 0.0 12.250279341580928 0.0 108-109 8.705880856002386E-4 0.0 0.0 12.947754334775272 0.0 110-111 8.87330164169474E-4 0.0 0.0 13.70255420499068 0.0 112-113 9.040722427387094E-4 0.0 0.0 14.55161197755088 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 9650 0.0 29.486244 4 ATGGGGA 5125 0.0 26.13498 5 ATGGGAG 4530 0.0 23.654177 5 AGTACTC 1520 0.0 23.498556 5 ATGGGGG 4305 0.0 23.231094 5 CATGGGT 5695 0.0 22.055029 4 GTATAGG 1010 0.0 21.802763 1 ATGGGAT 4495 0.0 20.130169 5 ATGGGTA 2945 0.0 19.405258 5 GCGCGAC 215 8.537401E-5 19.381104 9 TGGGGAG 3275 0.0 17.994623 6 GGCGCGA 265 2.077397E-5 17.970617 8 CTGGTCG 750 0.0 17.46145 9 TGGGGGG 2465 0.0 17.387394 6 AAGTACT 2265 0.0 17.345812 4 TCGCGGA 570 0.0 17.22126 82-83 GTCTAGA 900 0.0 17.19341 1 CGTTTAA 420 1.4708348E-8 17.004473 1 TGGGATA 1920 0.0 16.432117 6 TAGGACA 1055 0.0 16.363056 4 >>END_MODULE