FastQCFastQC Report
Thu 26 May 2016
SRR1364161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986487
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA552571.8502340710004765No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG316051.058266786361367No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC164980.5524216244704899No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164190.5497763760565507No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG157830.5284804521164833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157750.5282125788593756No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG139660.46763973859588204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104450.34974202131132665No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT103480.346494058068895No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC81000.27122167282161286No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT79250.26536194532238044No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC70760.2369338959118188No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA69290.2320117248124636No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG65180.21824973622855212No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61030.2043538110160868No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG60570.20281353978771713No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG47900.16038911269327474No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG47370.1586144523649358No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA46330.15513210002253483No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39660.13279816721117485No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC33120.11089952844261501No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC1602.604047E-729.751669
GTACCGT1954.7853973E-827.4639936
GTTATCA17700.027.2673191
GTATAGG10750.027.1592831
TACCGTA1651.1102689E-525.243417
AGTACTC14100.024.8960535
GAGTACT191050.024.3856412-13
CATGGGG81900.022.9562424
GTACTTT208900.022.39994614-15
AGTACTT196600.021.23066312-13
GGCGCGA2306.141645E-620.6968088
TTATCAA23950.019.8738522
ACTTTTT238200.019.8316216-17
GCGTATC1550.005643979619.220691
GTATACG1550.005691719219.1926944
ATGGGGA44750.018.2148285
ATGGGGG40050.018.1240355
ACCGTCC4301.0131771E-917.9893768
GTGTACG2652.0605285E-517.9876651
GTGTAGG14000.017.8752421
Produced by FastQC (version 0.11.5)