##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364160_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1650898 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.45558720163208 31.0 31.0 34.0 26.0 34.0 2 30.49696892236831 31.0 31.0 34.0 26.0 34.0 3 30.630920262790312 33.0 31.0 34.0 26.0 34.0 4 33.933310234793424 37.0 35.0 37.0 28.0 37.0 5 33.976500668121226 37.0 35.0 37.0 30.0 37.0 6 33.87830138506437 37.0 35.0 37.0 29.0 37.0 7 33.78402784423992 37.0 35.0 37.0 29.0 37.0 8 33.68308399428675 37.0 35.0 37.0 28.0 37.0 9 35.049592403649406 39.0 35.0 39.0 27.0 39.0 10-11 35.01906628998279 39.0 35.0 39.0 27.0 39.0 12-13 34.915731922868645 39.0 35.0 39.0 27.0 39.0 14-15 36.11599777817891 40.0 37.0 41.0 27.0 41.0 16-17 36.16350313586908 40.0 37.0 41.0 27.0 41.0 18-19 36.12058407000312 40.0 36.5 41.0 27.0 41.0 20-21 36.1476741749036 40.0 37.0 41.0 27.0 41.0 22-23 36.142113564859855 40.0 36.0 41.0 27.0 41.0 24-25 36.14430994525404 40.0 36.0 41.0 27.0 41.0 26-27 35.99064690853099 40.0 36.0 41.0 27.0 41.0 28-29 35.88634761202691 40.0 36.0 41.0 26.5 41.0 30-31 35.95435938501349 40.0 36.0 41.0 27.0 41.0 32-33 35.68916553294025 40.0 35.5 41.0 26.0 41.0 34-35 35.74852898240836 40.0 35.0 41.0 27.0 41.0 36-37 35.74990399164576 40.0 35.0 41.0 27.0 41.0 38-39 35.63444440540845 40.0 35.0 41.0 26.5 41.0 40-41 35.344162086331195 40.0 35.0 41.0 25.0 41.0 42-43 35.141644123380125 39.0 35.0 41.0 25.0 41.0 44-45 34.92800857472721 39.0 34.5 41.0 24.0 41.0 46-47 34.689337863393135 39.0 34.0 41.0 24.0 41.0 48-49 34.461375566509865 39.0 33.5 41.0 23.0 41.0 50-51 34.368063926420646 39.0 33.0 41.0 23.0 41.0 52-53 34.208523179505946 38.0 33.0 40.5 22.5 41.0 54-55 33.947352895212184 38.0 33.0 40.0 21.5 41.0 56-57 33.62491928635204 38.0 33.0 40.0 19.0 41.0 58-59 33.27985193512864 38.0 32.0 40.0 16.0 41.0 60-61 33.01448666119893 37.0 32.0 40.0 13.5 41.0 62-63 32.51421408227522 36.0 31.0 40.0 9.5 41.0 64-65 32.273044730807115 36.0 31.0 39.0 8.0 41.0 66-67 31.87945499964262 35.5 31.0 39.0 7.5 41.0 68-69 31.481431317985727 35.0 30.5 39.0 2.0 41.0 70-71 30.95473069808068 35.0 30.0 38.5 2.0 40.0 72-73 30.710060827501152 35.0 30.0 37.0 2.0 39.5 74-75 30.43242586761871 35.0 30.0 37.0 2.0 39.0 76-77 29.959549287720986 35.0 29.5 36.5 2.0 39.0 78-79 29.573110513187366 35.0 29.0 36.0 2.0 38.0 80-81 29.305264468186408 34.0 29.0 35.5 2.0 37.0 82-83 29.05344273237959 34.0 29.0 35.0 2.0 37.0 84-85 28.768320938059166 34.0 29.0 35.0 2.0 36.0 86-87 28.420346381181634 34.0 28.5 35.0 2.0 36.0 88-89 28.12724680749507 34.0 28.0 35.0 2.0 36.0 90-91 27.966874089132098 34.0 27.0 35.0 2.0 35.5 92-93 27.640301520748103 34.0 26.5 35.0 2.0 35.0 94-95 27.5597399112483 34.0 27.0 35.0 2.0 35.0 96-97 27.47078287089814 34.0 26.5 35.0 2.0 35.0 98-99 27.258558069608178 34.0 26.0 35.0 2.0 35.0 100-101 26.792761272955687 34.0 25.0 35.0 2.0 35.0 102-103 25.56520935878534 32.0 21.5 34.5 2.0 35.0 104-105 26.341543511470725 33.5 24.0 35.0 2.0 35.0 106-107 26.393123924070416 34.0 24.0 35.0 2.0 35.0 108-109 26.316500777152797 34.0 24.0 35.0 2.0 35.0 110-111 26.323174417801706 34.0 24.0 35.0 2.0 35.0 112-113 26.20888268081977 34.0 24.0 35.0 2.0 35.0 114-115 26.038112287979025 34.0 23.5 35.0 2.0 35.0 116-117 25.70394748797321 34.0 19.5 35.0 2.0 35.0 118-119 25.456669642824693 34.0 18.5 35.0 2.0 35.0 120-121 25.250512448376583 34.0 12.5 35.0 2.0 35.0 122-123 24.802638321689166 33.5 2.0 35.0 2.0 35.0 124-125 23.790630008637724 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40476117607423845 1101 2 0.451485491345597 1101 3 0.4389173839614209 1101 4 0.5107618684251136 1101 5 0.35028089587345335 1101 6 0.3734668965274466 1101 7 0.30588287602596864 1101 8 0.24226378339387367 1101 9 0.4038563937885087 1101 10-11 0.38008600456104347 1101 12-13 0.40466270236654367 1101 14-15 0.40346326189104786 1101 16-17 0.4787015454925694 1101 18-19 0.362285709651303 1101 20-21 0.443826711411333 1101 22-23 0.42518704058267787 1101 24-25 0.3952769917302774 1101 26-27 0.5248354870101295 1101 28-29 0.5201664276997349 1101 30-31 0.4003244058059465 1101 32-33 0.5027605102122621 1101 34-35 0.4844241384262915 1101 36-37 0.4812066973943061 1101 38-39 0.42329228867238555 1101 40-41 0.4799224754992366 1101 42-43 0.5105637763935462 1101 44-45 0.4777449535311149 1101 46-47 0.4678822007200907 1101 48-49 0.4595961446616954 1101 50-51 0.38151256226834107 1101 52-53 0.42730836521515414 1101 54-55 0.5209734976459472 1101 56-57 0.4481166980293665 1101 58-59 0.5030800081491336 1101 60-61 0.42072060597157446 1101 62-63 0.4778737892682159 1101 64-65 0.5351458333410761 1101 66-67 0.44513513546691996 1101 68-69 0.4009678789128195 1101 70-71 0.426878937464501 1101 72-73 0.48436358931708057 1101 74-75 0.5550473356771981 1101 76-77 0.39763642603083227 1101 78-79 0.5024369955062014 1101 80-81 0.41260009502767403 1101 82-83 0.4668770356935923 1101 84-85 0.5532263352922584 1101 86-87 0.4210534229346905 1101 88-89 0.43516978593986266 1101 90-91 0.4346730406031689 1101 92-93 0.5225248548537316 1101 94-95 0.4912867465903332 1101 96-97 0.4628641296132443 1101 98-99 0.5296222628385294 1101 100-101 0.47514820205817543 1101 102-103 0.43292444001592045 1101 104-105 0.4942429045548522 1101 106-107 0.42050597532961476 1101 108-109 0.4407651606920773 1101 110-111 0.39124223364792954 1101 112-113 0.36370513118031056 1101 114-115 0.3474629682414552 1101 116-117 0.16052790775264114 1101 118-119 0.23125182686791135 1101 120-121 0.09701341288328891 1101 122-123 0.01817235913273052 1101 124-125 0.015126228119097362 1102 1 0.39076214824041244 1102 2 0.4275777959653695 1102 3 0.4235725084242752 1102 4 0.4276835842901576 1102 5 0.3218302630163805 1102 6 0.47154360852610466 1102 7 0.34310099528880755 1102 8 0.42044913372443204 1102 9 0.5181474841083542 1102 10-11 0.40415132620528027 1102 12-13 0.5605476500486546 1102 14-15 0.6113964019465215 1102 16-17 0.5335871756716486 1102 18-19 0.6429441417898971 1102 20-21 0.5903616982524298 1102 22-23 0.6465841044427734 1102 24-25 0.6246144580077555 1102 26-27 0.762763832256212 1102 28-29 0.8435724424208786 1102 30-31 0.794148014589112 1102 32-33 0.9424650806311732 1102 34-35 0.8962590649402671 1102 36-37 0.7275402362194612 1102 38-39 0.7255155617363656 1102 40-41 0.7463035993002194 1102 42-43 0.8032274704434386 1102 44-45 0.7770698839816248 1102 46-47 0.6271924248877454 1102 48-49 0.6766363690686816 1102 50-51 0.7506325868283241 1102 52-53 0.7038218042983786 1102 54-55 0.6138864227520173 1102 56-57 0.6112735807265395 1102 58-59 0.5640886346796918 1102 60-61 0.6962729374785823 1102 62-63 0.8165131032391031 1102 64-65 0.7204343512993319 1102 66-67 0.7621556359591963 1102 68-69 0.7964312134758096 1102 70-71 0.6354571898266244 1102 72-73 0.6601572293036426 1102 74-75 0.6023053401617062 1102 76-77 0.6613097616914203 1102 78-79 0.7499008219291454 1102 80-81 0.6594179838755743 1102 82-83 0.4995640188176793 1102 84-85 0.488458363628677 1102 86-87 0.5655657594593322 1102 88-89 0.5123465757613346 1102 90-91 0.4850270394824534 1102 92-93 0.3665682738746874 1102 94-95 0.4136086887439703 1102 96-97 0.39392979044278675 1102 98-99 0.4398436245809023 1102 100-101 0.36042566141989596 1102 102-103 0.25222697268210936 1102 104-105 0.30891667183081495 1102 106-107 0.3306692381767711 1102 108-109 0.3412155921836586 1102 110-111 0.4230780349464034 1102 112-113 0.42580913645542395 1102 114-115 0.4349096455870942 1102 116-117 0.3196621021710193 1102 118-119 0.43862642527167495 1102 120-121 0.42157901223033534 1102 122-123 0.2862075683836558 1102 124-125 0.2233650475891622 1103 1 0.08331443921194648 1103 2 0.0906991896043472 1103 3 0.11133515580150188 1103 4 0.11647252611702896 1103 5 0.027072093853234946 1103 6 -0.017491436995804577 1103 7 0.10239541179101508 1103 8 0.08404947555308695 1103 9 0.22701699983223023 1103 10-11 0.2206916127495333 1103 12-13 0.26295581189497597 1103 14-15 0.24550900974827528 1103 16-17 0.23896944084433613 1103 18-19 0.1691016336019402 1103 20-21 0.32168308267464596 1103 22-23 0.31180562606537165 1103 24-25 0.36725588476239324 1103 26-27 0.5060691892157152 1103 28-29 0.527115510971754 1103 30-31 0.33330574813901137 1103 32-33 0.41855251697286633 1103 34-35 0.2331033914173588 1103 36-37 0.3397373261483736 1103 38-39 0.34567819714796855 1103 40-41 0.4089909966727987 1103 42-43 0.38038146392865 1103 44-45 0.36021792537943753 1103 46-47 0.2184605946410798 1103 48-49 0.19894335674813135 1103 50-51 0.20512870891645463 1103 52-53 0.3145556929550537 1103 54-55 0.3784696432339203 1103 56-57 0.37145943590858366 1103 58-59 0.4462262279892997 1103 60-61 0.32593481978377525 1103 62-63 0.3180254605713202 1103 64-65 0.21602558632108781 1103 66-67 0.37660493043229337 1103 68-69 0.22714938512908844 1103 70-71 0.28345015921646777 1103 72-73 0.4124063211696871 1103 74-75 0.31064553890418267 1103 76-77 0.2845782030966717 1103 78-79 0.2265293791745293 1103 80-81 0.26615058541962355 1103 82-83 0.3040189484509419 1103 84-85 0.32626042478754513 1103 86-87 0.14251722674347178 1103 88-89 0.21671920133363898 1103 90-91 0.19995964797950094 1103 92-93 0.1811200037556695 1103 94-95 0.30607180350801144 1103 96-97 0.265512494300971 1103 98-99 0.21834116313970497 1103 100-101 0.05877109107862566 1103 102-103 0.18338019163597252 1103 104-105 0.3315990404090954 1103 106-107 0.20845870309527115 1103 108-109 0.3356167331216824 1103 110-111 0.31377306767803503 1103 112-113 0.4003728157865112 1103 114-115 0.42490056626878214 1103 116-117 0.3220720426905679 1103 118-119 0.304426824179572 1103 120-121 0.2548131171575001 1103 122-123 0.38331706269877586 1103 124-125 0.35932593941591406 1104 1 0.2513501633595716 1104 2 0.46332381368620545 1104 3 0.3970345141675935 1104 4 0.292698433466704 1104 5 0.2579275680749191 1104 6 0.4471854365701873 1104 7 0.2882613602594759 1104 8 0.34262671637667097 1104 9 0.39525919654975894 1104 10-11 0.4148043831721182 1104 12-13 0.40502400181460274 1104 14-15 0.32032715385482646 1104 16-17 0.4523571944478775 1104 18-19 0.37830612114831297 1104 20-21 0.2870962600176412 1104 22-23 0.39525419807849005 1104 24-25 0.3345291979630858 1104 26-27 0.43429322074012333 1104 28-29 0.5254948738814917 1104 30-31 0.37455515507950565 1104 32-33 0.4900302298592649 1104 34-35 0.3638370844773675 1104 36-37 0.4051627726060829 1104 38-39 0.4345105884894309 1104 40-41 0.5308796307657531 1104 42-43 0.54295845183902 1104 44-45 0.5015311411300942 1104 46-47 0.4920066261002276 1104 48-49 0.4470654918627801 1104 50-51 0.43433891368201927 1104 52-53 0.511391202722649 1104 54-55 0.572981159884776 1104 56-57 0.6160832259416651 1104 58-59 0.6484617171723954 1104 60-61 0.49390872767567373 1104 62-63 0.6629681691399938 1104 64-65 0.5663907886014456 1104 66-67 0.58559125680204 1104 68-69 0.560537317591848 1104 70-71 0.5433679812509169 1104 72-73 0.5578431047914556 1104 74-75 0.49893950124458897 1104 76-77 0.48254876286953774 1104 78-79 0.38349332554939863 1104 80-81 0.49740457052849507 1104 82-83 0.5521922310259093 1104 84-85 0.3335968690161053 1104 86-87 0.4390244670900927 1104 88-89 0.43494133130216284 1104 90-91 0.41347456421062745 1104 92-93 0.4449194630360118 1104 94-95 0.41936029024606825 1104 96-97 0.40123879137546226 1104 98-99 0.3142128413788754 1104 100-101 0.36483608161477576 1104 102-103 0.18698953925795792 1104 104-105 0.29266303623697354 1104 106-107 0.3393822379558742 1104 108-109 0.2736009866072422 1104 110-111 0.32048303396464206 1104 112-113 0.31701414406409256 1104 114-115 0.19873755774378665 1104 116-117 0.21794593313584798 1104 118-119 0.18168029342080416 1104 120-121 0.017757190076835627 1104 122-123 -0.11523025529157493 1104 124-125 -0.10201622956274292 1105 1 0.14999503218673382 1105 2 0.26067142171089586 1105 3 0.23514215305648278 1105 4 0.10445576371361653 1105 5 0.31057513751936483 1105 6 -0.043473321145860666 1105 7 0.18157250995083984 1105 8 0.032455998784072904 1105 9 0.11986374901889008 1105 10-11 0.12939012777088266 1105 12-13 0.1553615789750964 1105 14-15 0.25746064150915515 1105 16-17 0.20207507639231892 1105 18-19 0.14094597454337787 1105 20-21 0.14609992514109393 1105 22-23 0.12674532462170163 1105 24-25 0.2215348885495061 1105 26-27 0.3527683364808638 1105 28-29 0.37145359301729286 1105 30-31 0.20430775924616995 1105 32-33 0.03461452769877127 1105 34-35 0.22318073802674832 1105 36-37 0.23866489451966544 1105 38-39 0.2568172050017665 1105 40-41 0.28924672181513245 1105 42-43 0.40097108686988037 1105 44-45 0.4396797715313312 1105 46-47 0.41214792625454066 1105 48-49 0.4188560975726503 1105 50-51 0.43679840442275975 1105 52-53 0.46834288096338383 1105 54-55 0.510329250162556 1105 56-57 0.4553000469293238 1105 58-59 0.5172454748884405 1105 60-61 0.5784525162559149 1105 62-63 0.6123532964548133 1105 64-65 0.6538980223977404 1105 66-67 0.628960276863765 1105 68-69 0.5661290421288889 1105 70-71 0.5567625607262272 1105 72-73 0.5491770168284944 1105 74-75 0.6306404078803922 1105 76-77 0.44688614712879726 1105 78-79 0.47275354931483093 1105 80-81 0.3584554000038054 1105 82-83 0.4094694300999855 1105 84-85 0.3525662941704262 1105 86-87 0.26651048175058634 1105 88-89 0.28063722250586665 1105 90-91 0.21693714137112252 1105 92-93 0.2831717778508285 1105 94-95 0.18507209580162254 1105 96-97 0.22152476745571192 1105 98-99 0.15241934215048403 1105 100-101 0.16795625908391187 1105 102-103 0.1339320546900602 1105 104-105 0.29280047836660117 1105 106-107 0.14780237684476205 1105 108-109 0.2901904927800807 1105 110-111 0.29662115124859056 1105 112-113 0.3445218601134741 1105 114-115 0.2956487267252683 1105 116-117 0.1022606515000426 1105 118-119 0.22015444465537115 1105 120-121 0.19397005658917976 1105 122-123 0.2104810680108926 1105 124-125 0.21993834219651376 1106 1 -0.04578709562225569 1106 2 0.05446228523278762 1106 3 0.044665169813555394 1106 4 0.07022030507355481 1106 5 -0.0041128770583185315 1106 6 0.09916201278478098 1106 7 0.06315047755060732 1106 8 0.010071415531463401 1106 9 0.06908794748847669 1106 10-11 0.02623448012946028 1106 12-13 0.1743903590022171 1106 14-15 0.15837658978501423 1106 16-17 0.06877350057383325 1106 18-19 0.07117514322798968 1106 20-21 0.029705026119394518 1106 22-23 0.050321155210617974 1106 24-25 0.10247984992590631 1106 26-27 0.311699740248784 1106 28-29 0.3107367560355101 1106 30-31 0.2002747566551193 1106 32-33 -0.005465776689064228 1106 34-35 0.1214360926615825 1106 36-37 0.1943241896087784 1106 38-39 0.009059628602116732 1106 40-41 0.04196565948463871 1106 42-43 0.03407909067170323 1106 44-45 0.08297686749703814 1106 46-47 0.22793358043412582 1106 48-49 0.07856882701376122 1106 50-51 0.07932582838633806 1106 52-53 0.17328653470709554 1106 54-55 0.2704061189876228 1106 56-57 0.3923550885798477 1106 58-59 0.41742179341808594 1106 60-61 0.31451790394479673 1106 62-63 0.3068584392047171 1106 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2216 84-85 -1.3217956941561688 2216 86-87 -1.4710992312506477 2216 88-89 -1.4514061872945234 2216 90-91 -1.361553064837107 2216 92-93 -1.5418177798347124 2216 94-95 -1.455329493628554 2216 96-97 -1.3403848982803126 2216 98-99 -1.338425976944663 2216 100-101 -1.2545679505977638 2216 102-103 -1.106344776562139 2216 104-105 -1.2303684755333322 2216 106-107 -1.2638916413578407 2216 108-109 -1.217828105531563 2216 110-111 -1.24633468325721 2216 112-113 -1.2389331170233504 2216 114-115 -1.3167307057624207 2216 116-117 -1.3040737801082933 2216 118-119 -1.2789571342225727 2216 120-121 -1.3483485906364585 2216 122-123 -1.3216755584620223 2216 124-125 -1.3361013688261707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54475.0 3 8813.0 4 6038.0 5 4368.0 6 5190.0 7 4896.0 8 5186.0 9 5976.0 10 7339.0 11 8743.0 12 9768.0 13 10632.0 14 11333.0 15 11233.0 16 11043.0 17 11199.0 18 11271.0 19 11679.0 20 11970.0 21 13340.0 22 15012.0 23 18537.0 24 21210.0 25 22694.0 26 26242.0 27 28007.0 28 32201.0 29 39479.0 30 46567.0 31 55728.0 32 70740.0 33 89058.0 34 110349.0 35 140762.0 36 208901.0 37 342177.0 38 157900.0 39 842.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.392376909531265 17.249130640942685 10.07646951049896 23.282022939027087 2 13.688834032929401 23.29164970169029 36.636923943956845 26.382592321423466 3 21.918634335256527 30.778817640160828 26.72361804764029 20.57892997694236 4 13.693371776163419 18.79978531083073 32.60695208317866 34.89989082982719 5 11.853305583954043 41.55828586728981 32.54198919234702 14.046419356409132 6 31.172583108001607 30.416162294835537 20.620120221552362 17.791134375610497 7 26.021002080492355 33.54929318634189 21.74294023041803 18.68676450274772 8 28.941041270258655 33.00172015207459 21.7458021837288 16.311436393937957 9 30.97530834134879 16.38957544470554 18.761338317119794 33.87377789682588 10-11 23.83083733588864 30.130588979094984 24.577581895492898 21.460991789523476 12-13 26.75312325543027 27.214131037619087 24.180185401531844 21.8525603054188 14-15 27.03574021743695 26.273043259156285 22.924912251658654 23.766304271748105 16-17 23.18503137989684 29.471905383440944 28.12626577858145 19.216797458080762 18-19 24.893031645350078 28.37933890483635 27.780097039816997 18.947532409996573 20-21 22.278333634093205 29.077352903316704 27.926573610649154 20.717739851940937 22-23 21.114978951434136 30.638378303191267 23.012987076232303 25.233655669142298 24-25 21.71220887415719 31.91836607998208 22.122003532523685 24.24742151333704 26-27 23.881284750849968 27.58285801764063 23.20940581810147 25.326451413407934 28-29 23.359759061151685 27.789499889372888 25.865257700301264 22.985483349174164 30-31 25.547229745483456 29.270315696942134 24.600668540430505 20.581786017143905 32-33 20.28991815328985 29.70414651443808 26.851838887688295 23.15409644458378 34-35 18.69301614935567 30.897071445528645 27.378484164135404 23.031428240980283 36-37 24.296402481114647 28.54921600415689 25.078071024325304 22.07631049040316 38-39 26.235071264725747 27.043100037617386 24.461580240441094 22.260248457215773 40-41 22.093761184508008 30.51530610848604 24.645797939481035 22.745134767524917 42-43 25.129466003104934 28.76564441058719 23.943691525903084 22.161198060404796 44-45 26.430449076666928 27.13613272462275 24.07927555855848 22.35414264015184 46-47 22.93463822816959 28.60431664306798 28.762398437923082 19.69864669083935 48-49 24.767867035673987 27.893740738182977 27.622917678855934 19.715474547287098 50-51 21.178071682762695 29.1270729928939 28.09025334780044 21.604601976542963 52-53 20.442118851416872 30.547883788752394 23.409358852536204 25.600638507294533 54-55 21.16375931648349 31.37085041399665 22.59277092895184 24.872619340568022 56-57 23.431662832750845 27.51672953802024 23.48649533057032 25.565112298658594 58-59 23.20065133087006 27.303616108942812 25.783925180890606 23.711807379296523 60-61 25.011733065832615 28.9756995507825 24.81460539334742 21.197961990037463 62-63 20.001076747783696 29.485034726636854 26.725822906014518 23.788065619564932 64-65 18.36696636494032 30.360839560780544 27.516803840356292 23.755390233922842 66-67 23.50360873786644 28.419844894840452 25.326562792768897 22.749983574524208 68-69 25.387327263933713 27.103630993481424 24.550102964839866 22.958938777744994 70-71 21.540078072903736 29.991901883192423 24.811388714474152 23.65663132942969 72-73 24.30365906970391 28.42288464738571 24.324923710439478 22.9485325724709 74-75 25.592113409291215 26.795248063810217 24.417576286196795 23.195062240701773 76-77 22.203622155893367 28.01485341876858 29.00425999992699 20.77726442541106 78-79 23.920122082347184 27.686436710845573 27.76348339846156 20.62995780834568 80-81 20.511418080934984 28.876078763008277 28.30500740722714 22.3074957488296 82-83 19.906113740356307 29.962109666597918 23.847608826719863 26.28416776632591 84-85 20.554242309296313 30.73744548574682 23.14561803809222 25.56269416686464 86-87 22.68981518521482 26.70954360759507 24.23509470111221 26.3655465060779 88-89 22.530720280662564 26.629424248148144 26.372158341720787 24.467697129468505 90-91 24.009964912921077 28.401279378014227 25.310665894051322 22.278089815013377 92-93 19.736931366879382 28.583875105979157 27.07587575212118 24.603317775020283 94-95 18.25758946972958 29.38719946703739 27.866004925036925 24.48920613819611 96-97 23.00157982687287 27.64988968581364 25.847153650707728 23.501376836605758 98-99 24.616241487783057 26.551093975118288 25.115991734268338 23.716672802830317 100-101 21.14059507801548 28.619884637472666 25.450105691726737 24.789414592785114 102-103 23.370824494495476 27.42194289181141 25.258067722392962 23.94916489130015 104-105 24.335829794425713 26.049916455335293 25.387060896567842 24.22719285367115 106-107 21.518886128687924 27.20456230715372 29.361005200940166 21.91554636321819 108-109 22.943391384564116 27.246913437606228 27.96617384234527 21.843521335484382 110-111 19.85433689837946 28.451865795323577 28.626053320601752 23.067743985695213 112-113 19.294518683851624 29.301558292674397 24.56692721835103 26.83699580512295 114-115 19.663790725812948 30.593602584971418 23.913418296895255 25.82918839232038 116-117 21.488598467230283 27.1960528595792 24.721003687524128 26.594344985666385 118-119 21.24608988295052 27.195187323584584 26.33491593818775 25.223806855277143 120-121 22.53434351793481 28.650459171130997 25.598608004400642 23.216589306533546 122-123 19.092025427934722 28.983393505318165 26.912082404803378 25.012498661943734 124-125 18.08566440743215 29.738394448419488 27.36752096727551 24.808420176872854 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8876.0 1 5439.5 2 3256.0 3 4559.0 4 4137.0 5 3258.5 6 2494.5 7 2022.0 8 1911.5 9 1835.0 10 1615.5 11 1431.0 12 1314.5 13 1294.0 14 1390.0 15 1449.0 16 1540.5 17 1715.5 18 1854.0 19 1995.5 20 2255.5 21 2759.0 22 3364.0 23 4100.0 24 4975.0 25 5906.5 26 7147.5 27 8551.0 28 9876.0 29 11500.0 30 13551.5 31 15625.0 32 17472.0 33 19722.0 34 22274.5 35 23902.5 36 25690.5 37 27656.0 38 28624.0 39 28803.5 40 29096.0 41 32009.5 42 35352.0 43 37885.0 44 40710.0 45 43265.5 46 46787.0 47 54613.5 48 66202.5 49 98136.5 50 205218.0 51 213370.5 52 99985.5 53 51105.5 54 39423.0 55 33626.5 56 28491.5 57 24450.5 58 21903.5 59 19432.0 60 17249.5 61 15345.0 62 13560.0 63 11173.5 64 8770.0 65 6842.5 66 5047.5 67 4239.5 68 3322.0 69 2180.0 70 1750.5 71 1585.0 72 1467.5 73 1254.0 74 942.0 75 770.0 76 525.5 77 274.0 78 168.5 79 137.5 80 103.5 81 71.0 82 62.5 83 52.5 84 38.5 85 35.5 86 36.0 87 27.5 88 19.0 89 15.0 90 15.5 91 12.5 92 11.0 93 6.5 94 3.5 95 4.5 96 3.5 97 2.5 98 1.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48585678824494305 2 0.47289414609503433 3 0.4618698429581961 4 0.4598709308509672 5 0.46144583129908695 6 0.4703500761403794 7 0.4565394106722523 8 0.4857962151507846 9 0.4990011496773271 10-11 0.5028475411563889 12-13 0.5028172546093096 14-15 0.47531706986137245 16-17 0.47777028017478973 18-19 0.4636870357829497 20-21 0.47474162546686716 22-23 0.49364043084430415 24-25 0.47731598196860137 26-27 0.4871591097693498 28-29 0.48406988196726874 30-31 0.49760796851168276 32-33 0.4962753604401968 34-35 0.49112664743672835 36-37 0.44596940574160243 38-39 0.4065363214444503 40-41 0.39999442727533746 42-43 0.40829294117504533 44-45 0.44306189722199674 46-47 0.4509666860096747 48-49 0.44760487928388065 50-51 0.43427879856902124 52-53 0.42788833713530455 54-55 0.43034154744872183 56-57 0.41189704027747326 58-59 0.41671260126307014 60-61 0.42640429632842247 62-63 0.4275854716645123 64-65 0.4422138739037784 66-67 0.4306746994665933 68-69 0.4320678806322377 70-71 0.4425470259216499 72-73 0.44366762816358124 74-75 0.44821061022546516 76-77 0.43830690933055827 78-79 0.42943295103634505 80-81 0.43812519004808287 82-83 0.43655028959996317 84-85 0.4347028102281304 86-87 0.44094183892645095 88-89 0.4284637815298098 90-91 0.4319164478968416 92-93 0.4419715815271446 94-95 0.4394880846666481 96-97 0.40768721023346083 98-99 0.38018702548552363 100-101 0.3789755636023546 102-103 0.3593498810950162 104-105 0.36159108557887887 106-107 0.3810956218979004 108-109 0.3895455685330045 110-111 0.40541571920251884 112-113 0.4016299008176156 114-115 0.40126646225266493 116-117 0.3733119792985394 118-119 0.44854376224333664 120-121 0.45441935237670644 122-123 0.40717233893311394 124-125 0.40035786584028815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1650898.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.50146964818804 #Duplication Level Percentage of deduplicated Percentage of total 1 87.10571278876182 49.21600787318011 2 8.866629424450679 10.01955186614662 3 2.0910567314340622 3.5444333533128294 4 0.7609501400123716 1.7197920495877383 5 0.3523795789109567 0.9954982041239353 6 0.20246392585685402 0.686370561699242 7 0.13097118568417213 0.5180045130905017 8 0.09563384899749003 0.4322762413176865 9 0.05926627025911168 0.3013768233185857 >10 0.3018038124451384 2.9824278828992736 >50 0.015762912954748295 0.6031113791499607 >100 0.011371326239706447 1.351713192791264 >500 0.0021243107891331264 0.8155459993405145 >1k 0.0014995134982116187 2.715497764750717 >5k 0.001124635123658714 4.427572471530055 >10k+ 0.0012495945818430155 19.670819823760894 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 73590 4.457573999120479 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 53204 3.222730901606277 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 35151 2.1292048327637443 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 19286 1.1682126939399042 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 19251 1.1660926356443584 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 18721 1.133988895740379 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 17688 1.071416889474698 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 15575 0.9434259415178889 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 14104 0.8543229200108063 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 12038 0.7291789074794445 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8132 0.4925804016965313 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 8061 0.4882797120112812 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7488 0.4535713290584882 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 7053 0.4272220330995616 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6943 0.4205589927421319 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 6852 0.4150468411737127 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 6519 0.3948760008189482 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6184 0.37458401427586685 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5478 0.33181940979999974 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 4831 0.29262861787948136 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 4821 0.2920228869378968 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 4806 0.29111429052552007 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 4643 0.2812408761776924 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3839 0.23254010847429704 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 3697 0.22393872910379684 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3391 0.20540336229131054 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2813 0.17039211386772532 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 1926 0.11666377934917846 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.057309415845195E-5 0.0 3 0.0 0.0 0.0 6.057309415845195E-5 0.0 4 0.0 0.0 0.0 6.057309415845195E-5 0.0 5 0.0 0.0 0.0 6.057309415845195E-5 0.0 6 0.0 0.0 0.0 6.057309415845195E-5 0.0 7 0.0 0.0 0.0 6.057309415845195E-5 0.0 8 0.0 0.0 0.0 6.057309415845195E-5 0.0 9 0.0 0.0 0.0 6.057309415845195E-5 0.0 10-11 0.0 0.0 0.0 2.422923766338078E-4 0.0 12-13 0.0 0.0 0.0 6.057309415845194E-4 0.0 14-15 0.0 0.0 0.0 0.0013931811656443947 0.0 16-17 0.0 0.0 0.0 0.0024532103134173038 0.0 18-19 0.0 0.0 0.0 0.003361806725794083 0.0 20-21 0.0 0.0 0.0 0.004664128250200799 0.0 22-23 0.0 0.0 0.0 0.008237940805549465 0.0 24-25 0.0 0.0 0.0 0.01150888789010587 0.0 26-27 0.0 0.0 0.0 0.015627858292880603 0.0 28-29 3.0286547079225974E-5 0.0 0.0 0.023048062327290966 3.0286547079225974E-5 30-31 6.057309415845195E-5 0.0 0.0 0.03725245290744795 6.057309415845195E-5 32-33 6.057309415845195E-5 0.0 0.0 0.06444977218459287 6.057309415845195E-5 34-35 6.057309415845195E-5 0.0 0.0 0.10536689728862716 6.057309415845195E-5 36-37 6.057309415845195E-5 0.0 0.0 0.16227531925049277 6.057309415845195E-5 38-39 6.057309415845195E-5 0.0 0.0 0.2471079376194047 6.057309415845195E-5 40-41 6.057309415845195E-5 0.0 0.0 0.37400856988136155 6.057309415845195E-5 42-43 6.057309415845195E-5 0.0 0.0 0.47604394699127384 6.057309415845195E-5 44-45 9.085964123767792E-5 0.0 0.0 0.5814714173740594 6.057309415845195E-5 46-47 1.211461883169039E-4 0.0 0.0 0.7178214523247348 6.057309415845195E-5 48-49 1.8171928247535585E-4 0.0 0.0 0.8405728276368376 6.057309415845195E-5 50-51 1.8171928247535585E-4 0.0 0.0 0.9692300796293896 6.057309415845195E-5 52-53 1.8171928247535585E-4 0.0 0.0 1.125962960764384 6.057309415845195E-5 54-55 1.8171928247535585E-4 0.0 0.0 1.2904491979516601 6.057309415845195E-5 56-57 1.8171928247535585E-4 0.0 0.0 1.4701998548668662 6.057309415845195E-5 58-59 1.8171928247535585E-4 0.0 0.0 1.6846588947348655 6.057309415845195E-5 60-61 1.8171928247535585E-4 0.0 0.0 1.8815214507498343 6.057309415845195E-5 62-63 1.8171928247535585E-4 0.0 0.0 2.075688504074752 6.057309415845195E-5 64-65 1.8171928247535585E-4 0.0 0.0 2.3069868641188007 6.057309415845195E-5 66-67 1.8171928247535585E-4 0.0 0.0 2.5593040878358324 6.057309415845195E-5 68-69 2.422923766338078E-4 0.0 0.0 2.8657736577305197 6.057309415845195E-5 70-71 2.422923766338078E-4 0.0 0.0 3.303656555401969 6.057309415845195E-5 72-73 2.422923766338078E-4 0.0 0.0 3.6642481849272333 6.057309415845195E-5 74-75 2.422923766338078E-4 6.057309415845195E-5 0.0 3.9977333548165905 6.057309415845195E-5 76-77 2.422923766338078E-4 6.057309415845195E-5 0.0 4.385673736354397 6.057309415845195E-5 78-79 2.422923766338078E-4 6.057309415845195E-5 0.0 4.766496779328584 6.057309415845195E-5 80-81 2.422923766338078E-4 6.057309415845195E-5 0.0 5.176273761310512 6.057309415845195E-5 82-83 2.422923766338078E-4 6.057309415845195E-5 0.0 5.677182963453829 6.057309415845195E-5 84-85 2.422923766338078E-4 6.057309415845195E-5 0.0 6.152409173673965 6.057309415845195E-5 86-87 2.422923766338078E-4 6.057309415845195E-5 0.0 6.641567195550543 6.057309415845195E-5 88-89 2.422923766338078E-4 6.057309415845195E-5 0.0 7.202534620551966 6.057309415845195E-5 90-91 2.422923766338078E-4 6.057309415845195E-5 0.0 7.736849884123671 6.057309415845195E-5 92-93 2.422923766338078E-4 6.057309415845195E-5 0.0 8.270710849489188 6.057309415845195E-5 94-95 2.422923766338078E-4 6.057309415845195E-5 0.0 8.856482956548497 6.057309415845195E-5 96-97 2.422923766338078E-4 6.057309415845195E-5 0.0 9.447706642082068 6.057309415845195E-5 98-99 2.422923766338078E-4 6.057309415845195E-5 0.0 10.129426530288363 6.057309415845195E-5 100-101 2.422923766338078E-4 6.057309415845195E-5 0.0 11.050713005891339 6.057309415845195E-5 102-103 2.422923766338078E-4 1.211461883169039E-4 0.0 11.831227610670071 6.057309415845195E-5 104-105 3.028654707922597E-4 1.211461883169039E-4 0.0 12.544051782726735 6.057309415845195E-5 106-107 3.028654707922597E-4 1.211461883169039E-4 0.0 13.312694061050411 6.057309415845195E-5 108-109 3.6343856495071165E-4 1.211461883169039E-4 0.0 14.018279748355138 6.057309415845195E-5 110-111 4.2401165910916364E-4 1.211461883169039E-4 0.0 14.748155246417404 6.057309415845195E-5 112-113 4.2401165910916364E-4 1.211461883169039E-4 0.0 15.61656141082005 6.057309415845195E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1550 0.0 46.089336 5 ATAACGC 175 0.0 44.22247 3 TATAACG 175 0.0 40.815773 2 AAGTACT 1735 0.0 40.48743 4 GCGCGAC 80 1.1941171E-4 37.20641 9 GTATACG 70 0.0027857162 34.009 1 GTACAAA 1535 0.0 32.568882 1 GGCGCGA 95 3.2771443E-4 31.33171 8 AAAGTAC 2260 0.0 30.55535 3 TAACGCA 290 0.0 28.73874 4 AAAAAGT 2575 0.0 26.579853 1 TACACCG 90 0.009505138 26.458693 5 AAAAGTA 2710 0.0 25.478477 2 CGTTTAA 285 1.1641532E-10 25.059263 1 CATGGGG 6510 0.0 24.41563 4 GAACAAA 1590 0.0 22.833088 1 ATGGGGC 965 0.0 22.825764 5 GTATAAC 495 0.0 21.642092 1 ATGGGAG 2345 0.0 21.324917 5 CGGTCCG 70 0.0031584566 21.248188 74-75 >>END_MODULE