##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364160_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1650898 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06496948933247 33.0 31.0 34.0 30.0 34.0 2 31.8611180097135 33.0 31.0 34.0 30.0 34.0 3 32.1017555294149 34.0 31.0 34.0 30.0 34.0 4 35.56676427011239 37.0 35.0 37.0 33.0 37.0 5 35.708505310443165 37.0 35.0 37.0 33.0 37.0 6 35.85626671060235 37.0 35.0 37.0 35.0 37.0 7 35.82576028319133 37.0 35.0 37.0 35.0 37.0 8 35.87757693085823 37.0 35.0 37.0 35.0 37.0 9 37.53713130671913 39.0 37.0 39.0 35.0 39.0 10-11 37.49978587411215 39.0 37.0 39.0 34.5 39.0 12-13 37.47752526200892 39.0 37.0 39.0 34.0 39.0 14-15 38.75691593302554 40.0 38.0 41.0 34.0 41.0 16-17 38.739582336401156 40.0 38.0 41.0 34.0 41.0 18-19 38.81096197342295 40.0 38.0 41.0 34.0 41.0 20-21 38.8479551734874 40.0 38.0 41.0 35.0 41.0 22-23 38.798207096986005 40.0 38.0 41.0 35.0 41.0 24-25 38.76349205099285 40.0 38.0 41.0 34.5 41.0 26-27 38.573203492886904 40.0 38.0 41.0 34.0 41.0 28-29 38.41472489517826 40.0 38.0 41.0 34.0 41.0 30-31 38.25900691623589 40.0 37.5 41.0 34.0 41.0 32-33 37.93707788125008 40.0 37.0 41.0 33.5 41.0 34-35 37.70386692575798 40.0 37.0 41.0 33.0 41.0 36-37 37.581172792019856 40.0 37.0 41.0 33.0 41.0 38-39 37.479619576739445 40.0 37.0 41.0 33.0 41.0 40-41 37.24627142318907 40.0 36.0 41.0 32.0 41.0 42-43 37.070773300349266 40.0 36.0 41.0 31.5 41.0 44-45 36.817951805623366 40.0 36.0 41.0 31.0 41.0 46-47 36.46706519724417 40.0 35.0 41.0 30.0 41.0 48-49 36.10807966331052 39.5 35.0 41.0 30.0 41.0 50-51 35.934548045972555 39.0 35.0 41.0 28.5 41.0 52-53 35.82459879411084 39.0 35.0 41.0 28.0 41.0 54-55 35.64246882605709 39.0 35.0 41.0 28.0 41.0 56-57 35.35943377483042 39.0 35.0 41.0 27.0 41.0 58-59 35.17548600822098 39.0 35.0 41.0 27.0 41.0 60-61 35.014746216907405 38.0 35.0 40.0 27.0 41.0 62-63 34.67803401542675 38.0 34.0 40.0 26.0 41.0 64-65 34.39089604566727 37.5 34.0 40.0 26.0 41.0 66-67 33.958632211075425 37.0 34.0 39.5 25.5 41.0 68-69 33.64075248743411 36.0 34.0 39.0 24.5 41.0 70-71 33.18696824394966 36.0 33.5 39.0 24.0 40.5 72-73 32.864743612264355 35.0 33.0 38.5 24.0 39.5 74-75 32.48651976076051 35.0 33.0 37.0 22.5 39.0 76-77 31.97920343958258 35.0 33.0 37.0 21.0 39.0 78-79 31.62882806811808 35.0 32.0 36.0 20.0 38.0 80-81 31.428075810861724 35.0 32.0 36.0 20.0 37.0 82-83 31.216988572280055 35.0 32.0 36.0 20.0 37.0 84-85 31.0031834189635 35.0 32.0 35.0 19.5 36.0 86-87 30.75111303060516 35.0 32.0 35.0 18.0 36.0 88-89 30.524682627273158 35.0 32.0 35.0 15.5 36.0 90-91 30.380826677359835 35.0 32.0 35.0 11.5 35.5 92-93 30.208519242254823 35.0 31.5 35.0 10.0 35.0 94-95 30.126127113849556 35.0 31.0 35.0 7.0 35.0 96-97 30.0564777472624 35.0 31.0 35.0 6.0 35.0 98-99 29.959071366008075 35.0 31.0 35.0 2.0 35.0 100-101 29.73371462077003 35.0 31.0 35.0 2.0 35.0 102-103 28.380306657346487 33.5 28.0 34.5 2.0 35.0 104-105 29.20904229092288 34.0 30.0 35.0 2.0 35.0 106-107 29.239011132123245 34.0 30.0 35.0 2.0 35.0 108-109 29.231175396662906 34.0 30.0 35.0 2.0 35.0 110-111 29.261458915087424 34.0 31.0 35.0 2.0 35.0 112-113 29.183069759609616 34.0 31.0 35.0 2.0 35.0 114-115 29.088254695323393 34.5 31.0 35.0 2.0 35.0 116-117 28.86079697231446 34.0 30.0 35.0 2.0 35.0 118-119 28.646633832011425 34.0 30.0 35.0 2.0 35.0 120-121 28.464894257549528 34.0 30.0 35.0 2.0 35.0 122-123 28.160671040851707 34.0 29.5 35.0 2.0 35.0 124-125 27.121495997935668 33.0 27.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40152900957078685 1101 2 0.6382184340119679 1101 3 0.4623172283847907 1101 4 0.5533119666275184 1101 5 0.2566623407829951 1101 6 0.29768116337041306 1101 7 0.3069042467784584 1101 8 0.26138211564624214 1101 9 0.3168325554376281 1101 10-11 0.3367722590502069 1101 12-13 0.3115023794025973 1101 14-15 0.4295941931329139 1101 16-17 0.5030093887840508 1101 18-19 0.3245421745557735 1101 20-21 0.2480215490254949 1101 22-23 0.29477950128685393 1101 24-25 0.27494847649792575 1101 26-27 0.25736261234996505 1101 28-29 0.34779262737445293 1101 30-31 0.23385583788216024 1101 32-33 0.19393657993965974 1101 34-35 0.11320409975600398 1101 36-37 0.03310569004187158 1101 38-39 -0.021388655594755335 1101 40-41 0.0662107336575346 1101 42-43 0.16165457696225616 1101 44-45 0.16318460258684553 1101 46-47 0.04608404986377934 1101 48-49 0.09597755346910475 1101 50-51 0.0913247073925092 1101 52-53 0.16011360781506312 1101 54-55 0.0824055570045914 1101 56-57 0.08669082057067357 1101 58-59 0.10787804557473635 1101 60-61 0.027716643796914298 1101 62-63 0.02010523720670676 1101 64-65 0.0060153672725036245 1101 66-67 -0.02457064901344097 1101 68-69 -0.022529239278235025 1101 70-71 0.07451239980196078 1101 72-73 0.15509514238927125 1101 74-75 0.18794552052943914 1101 76-77 0.18092600680948578 1101 78-79 0.17190501329529795 1101 80-81 0.1649719562801728 1101 82-83 0.1683631562878567 1101 84-85 0.19474320957595737 1101 86-87 0.18702026973727115 1101 88-89 0.2556237413793987 1101 90-91 0.299401576652059 1101 92-93 0.27590190834700934 1101 94-95 0.25020376235171327 1101 96-97 0.28900110103941756 1101 98-99 0.23141194892665595 1101 100-101 0.24975681107425274 1101 102-103 0.17665764846163867 1101 104-105 0.4568740618457454 1101 106-107 0.51465054762091 1101 108-109 0.40496999477357676 1101 110-111 0.23647606022861822 1101 112-113 0.2804364883086379 1101 114-115 0.2401995089132214 1101 116-117 0.22124726923050986 1101 118-119 0.2473135505395483 1101 120-121 0.27539972751025843 1101 122-123 0.2463533290981701 1101 124-125 0.18848451427182766 1102 1 0.30320141597916006 1102 2 0.5901858326091727 1102 3 0.4125683988216977 1102 4 0.4568123425372761 1102 5 0.3285526263011036 1102 6 0.2611194625912816 1102 7 0.2785016445421462 1102 8 0.25312580048614564 1102 9 0.27526296096626623 1102 10-11 0.32362416546146733 1102 12-13 0.23379452936706713 1102 14-15 0.3997680029102 1102 16-17 0.36995606901813005 1102 18-19 0.29574270074689935 1102 20-21 0.2504038513097555 1102 22-23 0.29676897581371264 1102 24-25 0.2597229347711121 1102 26-27 0.3970097440883791 1102 28-29 0.4446120429688918 1102 30-31 0.4555814410914678 1102 32-33 0.41040629630316516 1102 34-35 0.3099947960102014 1102 36-37 0.35111659513848537 1102 38-39 0.4238002359625028 1102 40-41 0.4064924967939447 1102 42-43 0.45593249599847496 1102 44-45 0.3817301165516298 1102 46-47 0.28369590467926287 1102 48-49 0.42189527255929704 1102 50-51 0.3329842561192464 1102 52-53 0.380080976702601 1102 54-55 0.2698588364974057 1102 56-57 0.27438788434823635 1102 58-59 0.3861574765026603 1102 60-61 0.3450533046124278 1102 62-63 0.3575211733045265 1102 64-65 0.30746041223724774 1102 66-67 0.27916220500401323 1102 68-69 0.41578145281660284 1102 70-71 0.2710654187575585 1102 72-73 0.2591308699087449 1102 74-75 0.23064342195443288 1102 76-77 0.319095082847241 1102 78-79 0.24331680819629398 1102 80-81 0.2264970357741518 1102 82-83 0.3155545616126538 1102 84-85 0.38137031287562806 1102 86-87 0.3842084152518943 1102 88-89 0.4139898584998356 1102 90-91 0.35716904407664885 1102 92-93 0.29419456007466493 1102 94-95 0.22131804799526122 1102 96-97 0.2455691233744801 1102 98-99 0.19970109326362362 1102 100-101 0.1087150582701959 1102 102-103 0.1407990763575242 1102 104-105 0.1623560722325763 1102 106-107 0.23292207247758512 1102 108-109 0.30439874398853917 1102 110-111 0.2868839491342854 1102 112-113 0.2838814061820507 1102 114-115 0.4072780670372893 1102 116-117 0.38184350914263376 1102 118-119 0.3312285743031502 1102 120-121 0.4179193709612967 1102 122-123 0.30593118780727124 1102 124-125 0.2567726682867182 1103 1 0.21742021057232108 1103 2 0.6211763029631356 1103 3 0.43229307637653847 1103 4 0.5236526184903383 1103 5 0.20942808926144352 1103 6 0.20436345532458944 1103 7 0.21507512759664138 1103 8 0.2166859604697109 1103 9 0.28994105749486465 1103 10-11 0.30497388738208286 1103 12-13 0.23462743352811088 1103 14-15 0.3824794608805533 1103 16-17 0.37501421142032143 1103 18-19 0.19971181004395078 1103 20-21 0.3054140099593212 1103 22-23 0.2858756533572304 1103 24-25 0.24568127986120913 1103 26-27 0.15740491676925217 1103 28-29 0.2853191625935594 1103 30-31 0.32257112559526036 1103 32-33 0.3121586936717051 1103 34-35 0.4330550517995846 1103 36-37 0.4368588314319908 1103 38-39 0.392307290423048 1103 40-41 0.4805777938768472 1103 42-43 0.5335861349617659 1103 44-45 0.4076316411171277 1103 46-47 0.43737553490927183 1103 48-49 0.327288614015238 1103 50-51 0.5602401594952511 1103 52-53 0.5452943325981465 1103 54-55 0.5878850428218811 1103 56-57 0.438029782451288 1103 58-59 0.2508669827833927 1103 60-61 0.37959724067074063 1103 62-63 0.2545465040366821 1103 64-65 0.328138620337171 1103 66-67 0.3311016460316978 1103 68-69 0.4023925709795222 1103 70-71 0.4173482746923014 1103 72-73 0.285507461922343 1103 74-75 0.3155486307989648 1103 76-77 0.20911955577381036 1103 78-79 0.17751794541527133 1103 80-81 0.24190834991452448 1103 82-83 0.15811707995522184 1103 84-85 0.21977776972056873 1103 86-87 0.23757928398014982 1103 88-89 0.1510312134607581 1103 90-91 0.20007549314151163 1103 92-93 0.11963459978758806 1103 94-95 0.2200512284165974 1103 96-97 0.12458554942169542 1103 98-99 0.11163768841415234 1103 100-101 0.12486710895914754 1103 102-103 0.0018465370185296592 1103 104-105 0.09664695160053327 1103 106-107 0.05859633111445817 1103 108-109 0.1383182848968083 1103 110-111 0.25884591463287876 1103 112-113 0.23635582360593688 1103 114-115 0.2020421381615769 1103 116-117 0.4072140189022946 1103 118-119 0.13204700653206203 1103 120-121 0.17425739002058904 1103 122-123 0.023674665938077766 1103 124-125 0.1158297127624408 1104 1 0.24804592914706802 1104 2 0.5247314907590379 1104 3 0.0631985684213845 1104 4 0.3946034968459884 1104 5 0.1594843200889926 1104 6 0.16998294834343142 1104 7 0.14335619010825695 1104 8 0.1464350806078798 1104 9 0.23137658784695958 1104 10-11 0.20055179720189642 1104 12-13 0.1842416153695723 1104 14-15 0.16870308071497675 1104 16-17 0.21701829443108522 1104 18-19 0.17120274909876798 1104 20-21 0.1350503703437056 1104 22-23 0.09413363047831069 1104 24-25 0.10466953602777096 1104 26-27 0.26258511899941084 1104 28-29 0.3713198516722258 1104 30-31 0.2296360332913565 1104 32-33 0.2310322320467293 1104 34-35 0.2274013903945047 1104 36-37 0.18038734410149004 1104 38-39 0.08194803128930062 1104 40-41 0.13045405153954448 1104 42-43 0.07319458703251058 1104 44-45 0.13444952540713473 1104 46-47 0.17958323501822093 1104 48-49 0.04491878273282168 1104 50-51 0.06981658007673985 1104 52-53 0.04318591190619969 1104 54-55 0.06896228678334637 1104 56-57 0.23263201524189014 1104 58-59 0.32052150836089055 1104 60-61 0.19124452150857962 1104 62-63 0.17380019669504776 1104 64-65 0.13224219990630104 1104 66-67 -0.023278474990092946 1104 68-69 0.07744020401312213 1104 70-71 -0.017288408893818996 1104 72-73 -0.060625131122108655 1104 74-75 -0.029488763328934908 1104 76-77 0.021821164628164524 1104 78-79 -0.05807721961216572 1104 80-81 -0.04584867391831082 1104 82-83 -0.1169046184919047 1104 84-85 -0.1296049620182167 1104 86-87 -0.04658000239040305 1104 88-89 -0.12809789277032024 1104 90-91 -0.12285490175453617 1104 92-93 -0.07715611672870892 1104 94-95 -0.0913861281822399 1104 96-97 -0.1404510978905229 1104 98-99 -0.08868494851042286 1104 100-101 -0.04437120808230688 1104 102-103 -0.15769015798947805 1104 104-105 -0.07901805952011642 1104 106-107 -0.11286125226531851 1104 108-109 -0.11483301054598272 1104 110-111 -0.0821167841205046 1104 112-113 -0.10225770053566308 1104 114-115 -0.16229047847973632 1104 116-117 -0.1666348337628989 1104 118-119 -0.2123592812794044 1104 120-121 -0.19959061541672796 1104 122-123 -0.2920743744979113 1104 124-125 -0.21974806840424677 1105 1 0.20362077482607788 1105 2 0.5603989290306473 1105 3 0.17166693261891908 1105 4 0.37325897524105045 1105 5 0.15805685095318722 1105 6 0.09901940667676712 1105 7 0.14652941541689302 1105 8 0.04306895406467248 1105 9 0.1503243116123869 1105 10-11 0.20535021541176945 1105 12-13 0.16309722879549327 1105 14-15 0.2490150984618893 1105 16-17 0.2518393785360189 1105 18-19 0.17843172286420383 1105 20-21 0.1877702777511132 1105 22-23 0.17376326010794685 1105 24-25 0.17222837090431398 1105 26-27 0.2172243936907634 1105 28-29 0.2963632544500072 1105 30-31 0.24867056261234666 1105 32-33 0.1058345082813048 1105 34-35 0.2142551712587064 1105 36-37 0.2041383086076678 1105 38-39 0.1369514070609057 1105 40-41 0.1988061927432483 1105 42-43 0.12160797437819326 1105 44-45 0.19591027000590344 1105 46-47 0.08007272190093317 1105 48-49 0.08294926112787948 1105 50-51 -0.010577420694865225 1105 52-53 0.026895402646943012 1105 54-55 0.15317573199939716 1105 56-57 0.24268940335917222 1105 58-59 0.21159741499668883 1105 60-61 0.12625127305178552 1105 62-63 0.10945798990491795 1105 64-65 0.05518539049271709 1105 66-67 0.08317407130619614 1105 68-69 0.10364101419830973 1105 70-71 0.0779541644086521 1105 72-73 0.019554267026045125 1105 74-75 0.01573210858465046 1105 76-77 -0.00821838012492293 1105 78-79 5.8389955736259935E-5 1105 80-81 0.07530535771749314 1105 82-83 -0.0033123152511649323 1105 84-85 0.16270068998795395 1105 86-87 0.18155946520219501 1105 88-89 0.2568413433407919 1105 90-91 0.24786510210348922 1105 92-93 0.19473817339474664 1105 94-95 0.2047630809226959 1105 96-97 0.19066145997984663 1105 98-99 0.24634350442167374 1105 100-101 0.13029844855349637 1105 102-103 0.036751875189533934 1105 104-105 0.14024071748605493 1105 106-107 0.0941073048334502 1105 108-109 0.07576546553426411 1105 110-111 0.09838331233011388 1105 112-113 0.10731502322976638 1105 114-115 0.1125262653474266 1105 116-117 0.19107639308895585 1105 118-119 0.1546377287514602 1105 120-121 0.09086800726845823 1105 122-123 0.03693073738480379 1105 124-125 -0.02315518645980319 1106 1 0.23546586878191533 1106 2 0.5422561306966642 1106 3 0.4269925089459221 1106 4 0.4813060621259808 1106 5 0.1841816572298285 1106 6 0.12095479388133867 1106 7 0.05606393449864555 1106 8 0.053244576400977905 1106 9 0.2789425494562465 1106 10-11 0.22418754227150828 1106 12-13 0.3171427931358721 1106 14-15 0.3589465234182967 1106 16-17 0.24002369118131384 1106 18-19 0.20265334844536653 1106 20-21 0.12639447864223996 1106 22-23 0.13853103422650292 1106 24-25 0.19950870677219257 1106 26-27 0.2370456233498146 1106 28-29 0.3552313954321775 1106 30-31 0.25666059191300405 1106 32-33 0.25959955211121155 1106 34-35 0.23383424671395403 1106 36-37 0.17310246373165228 1106 38-39 0.24253132097508967 1106 40-41 0.30265954718436205 1106 42-43 0.29224511235765505 1106 44-45 0.29398960880384806 1106 46-47 0.2853475215183323 1106 48-49 0.39343241930301076 1106 50-51 0.282102333397539 1106 52-53 0.2758084329646522 1106 54-55 0.3394418806578585 1106 56-57 0.3718369751578976 1106 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104-105 -0.9471747135067332 2216 106-107 -0.9617743287842089 2216 108-109 -1.156403939033975 2216 110-111 -1.1023544509261285 2216 112-113 -1.0737397329696705 2216 114-115 -1.1786381626335825 2216 116-117 -1.2340968668916972 2216 118-119 -1.2119206905019695 2216 120-121 -1.3691638280507483 2216 122-123 -1.37723446827383 2216 124-125 -1.5979216770727156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60.0 3 23.0 4 68.0 5 265.0 6 757.0 7 1347.0 8 2123.0 9 4264.0 10 8974.0 11 11482.0 12 9841.0 13 12026.0 14 17029.0 15 16613.0 16 9745.0 17 8603.0 18 9071.0 19 8840.0 20 8772.0 21 8308.0 22 8769.0 23 9524.0 24 10755.0 25 12430.0 26 14913.0 27 18352.0 28 22584.0 29 28097.0 30 35433.0 31 44655.0 32 58033.0 33 77422.0 34 109189.0 35 171929.0 36 274609.0 37 427651.0 38 187294.0 39 1048.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.422030675162944 17.978059706471992 11.272955570450664 23.3269540479144 2 13.323011615566896 23.323387171025534 36.50683753228565 26.846763681121917 3 22.164017997543144 28.866697518674755 28.839197233980173 20.130087249801925 4 13.736281708500464 18.736832923657307 33.564278350328124 33.96260701751411 5 11.532995981580934 38.98660002011027 34.795971646946086 14.684432351362714 6 31.47924692883623 30.279736376632506 19.715962782583414 18.525053911947857 7 23.14528214341528 32.93207696659636 24.220151699257013 19.70248919073135 8 27.560939561378113 31.964240068132614 23.414105535290492 17.06071483519878 9 28.802506272343898 14.454920897596338 21.59273316704 35.14983966301976 10-11 23.73860279030043 27.175460620662395 26.87204990013008 22.213886688907095 12-13 27.14677815934107 24.997470940040987 27.019265076377614 20.836485824240327 14-15 27.012614522601652 23.095025365336564 26.77071250094647 23.121647611115318 16-17 22.021061366899065 26.945132625432343 31.73909224721211 19.29471376045648 18-19 23.712791462585816 24.69368186284071 32.9721157818351 18.62141089273838 20-21 20.97796560838308 24.994268265757423 33.597775511668964 20.429990614190533 22-23 19.97267547722512 26.37906763470548 29.310502526503758 24.337754361565644 24-25 21.080587655930287 27.58913633670887 28.204316680982107 23.125959326378734 26-27 22.830514059620885 23.724633502493795 29.432436165044724 24.0124162728406 28-29 22.03316321354077 23.24087139373752 32.345233238566585 22.380732154155123 30-31 24.163122131106828 24.8873037583182 30.94842927909538 20.001144831479596 32-33 19.450959417238376 25.20976462507072 32.576210038415454 22.763065919275448 34-35 18.045875638591845 26.500274396116534 33.42611112255269 22.027738842738923 36-37 23.34865630705228 24.77818132919175 30.293846137072066 21.5793162266839 38-39 24.520897111753726 23.472982582812506 30.21476796264821 21.791352342785565 40-41 21.403957119095182 25.785875323611755 30.33424839087575 22.475919166417306 42-43 24.319067562017764 24.80674154308746 29.501247200008724 21.372943694886057 44-45 25.652160218256974 22.87854852328854 29.337760418875057 22.13153083957943 46-47 22.193042816697336 24.860500164153084 33.572576864227834 19.373880154921746 48-49 24.286933535566703 24.534435198298137 31.72346201885277 19.455169247282388 50-51 20.843201699923313 25.52471442814759 31.9329237784527 21.6991600934764 52-53 20.136646843112054 26.967959256113943 27.614577036255422 25.280816864518584 54-55 21.198856622274665 27.78000215640215 26.61318264362789 24.40795857769529 56-57 23.032434468998083 24.32812323959445 27.62593449141013 25.013507799997335 58-59 22.55563335832983 23.55272706127211 30.24405505367382 23.647584526724245 60-61 24.370524405505368 25.30074541249671 29.096830936859817 21.2318992451381 62-63 19.812974514476362 25.60639724562026 30.69508231277765 23.885545927125722 64-65 18.30391701970685 26.581836067400893 31.87447074258979 23.239776170302466 66-67 23.154095528615333 25.32194599545217 28.81846728265465 22.70549119327784 68-69 24.3175532346638 24.01595979884887 28.732604921685045 22.933882044802285 70-71 21.39741522492607 26.099795384087933 28.875860289369786 23.626929101616213 72-73 23.922495514562378 25.320492241192373 28.13780742359613 22.619204820649124 74-75 25.203979894578588 23.319611508403305 28.20495269847077 23.271455898547337 76-77 21.785264746822637 25.051093404922653 32.47786962004921 20.685772228205497 78-79 23.53394940208299 24.79738300003998 30.970811037386923 20.69785656049011 80-81 20.23883365295736 25.82237061284222 31.24223907231095 22.69655666188947 82-83 19.659633726614242 26.862471212637 27.249230418838717 26.228664641910036 84-85 20.62813692911373 27.430767982031597 26.416744099272034 25.52435098958264 86-87 22.288142574526105 23.91489359124549 27.51278395152214 26.284179882706262 88-89 21.796258763412396 23.41725533618673 29.874619752401422 24.911866147999454 90-91 23.32451792903014 25.17908435287946 28.619090943232106 22.87730677485829 92-93 19.39392984908819 25.22575592192855 30.025022745196857 25.355291483786402 94-95 17.910432391799073 26.201581331732577 31.151072120623642 24.736914155844705 96-97 22.32576805014379 24.962012051349227 28.476468975877616 24.235750922629368 98-99 23.329582711805372 23.9326402507818 28.220887882665618 24.51688915474721 100-101 20.67898804165975 25.45417706000007 28.632417023946964 25.234417874393213 102-103 22.759906350422664 24.586527424000582 28.24385841104653 24.409707814530222 104-105 23.853150145134055 22.667843249161034 28.537487360220243 24.941519245484667 106-107 20.860964264466936 24.351518757126083 32.16276733796754 22.624749640439447 108-109 22.320012141109498 24.446311789629227 30.427777210640677 22.805898858620598 110-111 19.33366815113328 25.30604628845987 30.89390900185321 24.466376558553634 112-113 18.693728964859822 26.102884269660535 27.387158049762505 27.816228715717134 114-115 19.31513681023753 26.95450121860915 26.55601420882513 27.174347762328193 116-117 20.831107651688978 23.844693222545644 27.514787969169397 27.80941115659598 118-119 20.281322865139455 23.569431153206846 29.4258457998326 26.7234001818211 120-121 21.673863228472033 24.93311059583291 28.399758280337256 24.9932678953578 122-123 18.22572667846059 25.277516044002773 29.3814915402697 27.11526573726694 124-125 17.03666372074344 26.19226601262729 30.260365904260123 26.510704362369154 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2498.0 1 1886.5 2 3951.0 3 5295.0 4 2585.5 5 1288.5 6 2085.0 7 4309.5 8 6267.5 9 6351.5 10 4728.0 11 2969.0 12 2472.0 13 2519.0 14 2830.5 15 3278.0 16 3536.5 17 3889.0 18 4169.5 19 4158.5 20 4220.0 21 4546.0 22 5064.0 23 5615.5 24 6220.5 25 7050.5 26 8220.0 27 9316.5 28 10213.5 29 11377.0 30 13102.5 31 15099.5 32 16988.5 33 18933.0 34 20875.0 35 22146.0 36 23968.5 37 25960.5 38 26588.0 39 26863.5 40 27088.0 41 29835.5 42 33114.0 43 35538.0 44 38345.5 45 41073.5 46 44974.5 47 53955.5 48 65436.0 49 100210.5 50 224848.0 51 226324.0 52 90983.5 53 42775.5 54 34620.5 55 30606.5 56 26547.5 57 23135.0 58 20811.5 59 18459.0 60 16414.0 61 14429.0 62 12538.0 63 10333.5 64 8339.5 65 6507.0 66 4645.0 67 3901.5 68 3046.5 69 1986.0 70 1638.5 71 1387.0 72 1235.5 73 1114.0 74 913.0 75 771.0 76 444.5 77 179.5 78 90.5 79 54.5 80 27.0 81 13.0 82 8.5 83 4.5 84 2.5 85 2.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1413170286716684 2 6.057309415845194E-4 3 3.634385649507117E-4 4 0.0 5 0.0 6 0.0035132394611902132 7 0.0 8 0.0 9 0.0 10-11 3.028654707922597E-5 12-13 0.005269859191785319 14-15 0.0013931811656443947 16-17 4.845847532676155E-4 18-19 0.0 20-21 9.085964123767792E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.5143273539612985E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 6.663040357429715E-4 96-97 0.0027560757842095634 98-99 0.003664672196586343 100-101 0.0 102-103 0.0038161049319824724 104-105 0.02150344842625044 106-107 0.01647588161109893 108-109 0.018626226453723974 110-111 0.015082700445454535 112-113 0.014386109862632338 114-115 0.03973594976794448 116-117 0.0396450901267068 118-119 0.022684623762340254 120-121 0.013659232732730913 122-123 0.0028772219725264675 124-125 0.0017566197305951066 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1650898.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.559614581146384 #Duplication Level Percentage of deduplicated Percentage of total 1 86.60425296332804 46.38490409803954 2 9.280758741669354 9.941477224488313 3 2.0937699209027567 3.36424529955447 4 0.7519728668874195 1.6110150770387954 5 0.36670202070531577 0.9820209447552137 6 0.22060034113909557 0.7089161548727624 7 0.13354958501722605 0.5007005010696268 8 0.10100578154157672 0.4327864583867455 9 0.06796427614862219 0.3276126392226124 >10 0.3262598976761105 3.1066863803046707 >50 0.026404520740786825 0.9571309609208986 >100 0.018731364592375087 2.3290278433802483 >500 0.0029307547933990065 1.0632335407625033 >1k 0.0019113618217819607 2.4129716327876674 >5k 0.0020387859432340915 8.018305554727839 >10k+ 0.0011468170930691763 17.858965689688066 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 64998 3.9371299741110595 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 56593 3.4280131177092708 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 35304 2.138472516169988 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 22086 1.3378173575835697 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 20023 1.2128550643346832 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 17849 1.0811691576342086 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 17596 1.0658441648121204 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 15688 0.9502707011577941 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 11541 0.6990740796826939 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9785 0.5927077263404523 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 9375 0.5678727577354871 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 8835 0.535163286889923 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8724 0.5284396734383348 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8553 0.5180816743372395 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 7956 0.48191953712464364 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 7699 0.46635225192592156 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 7680 0.46520136313691096 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7382 0.4471505810776923 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6646 0.40256878377707167 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 6619 0.40093331023479345 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 6209 0.37609834162982814 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 6052 0.36658836584695115 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 5591 0.33866416943990485 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5340 0.3234603228061334 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 5042 0.30540954074691473 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 4282 0.25937398918649124 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3968 0.2403540376207373 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3502 0.2121269757428987 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3148 0.19068410041080672 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3097 0.18759487260872568 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2651 0.1605792726140561 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2551 0.1545219631982109 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2198 0.13313966096027738 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 2175 0.131746479794633 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.057309415845195E-5 0.0 3 0.0 0.0 0.0 6.057309415845195E-5 0.0 4 0.0 0.0 0.0 6.057309415845195E-5 0.0 5 0.0 0.0 0.0 6.057309415845195E-5 0.0 6 0.0 0.0 0.0 6.057309415845195E-5 0.0 7 0.0 0.0 0.0 6.057309415845195E-5 0.0 8 0.0 0.0 0.0 6.057309415845195E-5 0.0 9 0.0 0.0 0.0 6.057309415845195E-5 0.0 10-11 0.0 0.0 0.0 1.211461883169039E-4 0.0 12-13 0.0 0.0 0.0 4.845847532676155E-4 0.0 14-15 0.0 0.0 0.0 0.001241748430248265 0.0 16-17 0.0 0.0 0.0 0.0022714910309419483 0.0 18-19 0.0 0.0 0.0 0.003180087443318727 0.0 20-21 0.0 0.0 0.0 0.004573268608963121 0.0 22-23 0.0 0.0 0.0 0.008147081164311787 0.0 24-25 6.057309415845195E-5 0.0 0.0 0.01150888789010587 0.0 26-27 6.057309415845195E-5 0.0 0.0 0.015597571745801377 0.0 28-29 6.057309415845195E-5 0.0 0.0 0.023199495062687096 0.0 30-31 6.057309415845195E-5 0.0 0.0 0.037494745284081754 0.0 32-33 6.057309415845195E-5 0.0 0.0 0.06484349729662281 0.0 34-35 6.057309415845195E-5 0.0 0.0 0.10606348787144936 0.0 36-37 6.057309415845195E-5 0.0 0.0 0.1633959214924241 0.0 38-39 6.057309415845195E-5 0.0 0.0 0.2491977093678713 0.0 40-41 6.057309415845195E-5 0.0 0.0 0.3776126689837894 0.0 42-43 6.057309415845195E-5 0.0 0.0 0.4795874729995433 0.0 44-45 6.057309415845195E-5 0.0 0.0 0.5858023936063887 0.0 46-47 6.057309415845195E-5 0.0 0.0 0.7224855805749355 0.0 48-49 6.057309415845195E-5 0.0 0.0 0.846387844676049 0.0 50-51 6.057309415845195E-5 0.0 0.0 0.9753479621393932 0.0 52-53 6.057309415845195E-5 0.0 0.0 1.1328380069513684 0.0 54-55 6.057309415845195E-5 0.0 0.0 1.2985659925688928 0.0 56-57 6.057309415845195E-5 0.0 0.0 1.4795886844614263 0.0 58-59 6.057309415845195E-5 0.0 0.0 1.6973792445081404 0.0 60-61 6.057309415845195E-5 0.0 0.0 1.895271543123803 0.0 62-63 6.057309415845195E-5 0.0 0.0 2.0908014910672854 0.0 64-65 9.085964123767792E-5 0.0 0.0 2.324007903577326 0.0 66-67 1.211461883169039E-4 0.0 0.0 2.578263466307428 0.0 68-69 1.211461883169039E-4 0.0 0.0 2.885883924991126 0.0 70-71 1.211461883169039E-4 0.0 0.0 3.325614302034408 0.0 72-73 1.211461883169039E-4 0.0 0.0 3.6875385396311584 0.0 74-75 1.211461883169039E-4 0.0 0.0 4.023325487098537 0.0 76-77 1.211461883169039E-4 0.0 0.0 4.414143090608869 0.0 78-79 1.211461883169039E-4 0.0 0.0 4.797207338066919 0.0 80-81 1.211461883169039E-4 0.0 0.0 5.210194694039244 0.0 82-83 1.211461883169039E-4 0.0 0.0 5.7137085392313764 0.0 84-85 1.211461883169039E-4 0.0 0.0 6.193114292948444 0.0 86-87 1.211461883169039E-4 0.0 0.0 6.686512431416114 0.0 88-89 1.211461883169039E-4 0.0 0.0 7.2512656748024416 0.0 90-91 1.211461883169039E-4 0.0 0.0 7.789851341512316 0.0 92-93 1.211461883169039E-4 0.0 0.0 8.328194715845559 0.0 94-95 1.211461883169039E-4 0.0 0.0 8.921477886580515 0.0 96-97 1.211461883169039E-4 0.0 0.0 9.519031460453643 0.0 98-99 1.211461883169039E-4 0.0 0.0 10.20898928946549 0.0 100-101 1.211461883169039E-4 0.0 0.0 11.139846313945501 0.0 102-103 1.211461883169039E-4 0.0 0.0 11.929658888677556 0.0 104-105 1.211461883169039E-4 0.0 0.0 12.652356475082046 0.0 106-107 1.211461883169039E-4 0.0 0.0 13.429993857888253 0.0 108-109 1.211461883169039E-4 0.0 0.0 14.147058146536008 0.0 110-111 1.211461883169039E-4 0.0 0.0 14.890804883160559 0.0 112-113 1.211461883169039E-4 0.0 0.0 15.776110940833414 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1610 0.0 51.734837 5 TATAACG 215 0.0 49.81133 2 ATAACGC 230 0.0 46.562763 3 AAGTACT 1880 0.0 44.93776 4 GTTATCA 850 0.0 44.16724 1 AAAGTAC 1905 0.0 42.475452 3 TAACGCA 270 0.0 39.663376 4 AAAAGTA 2385 0.0 33.67747 2 AAAAAGT 2390 0.0 33.410698 1 TTATCAA 1110 0.0 33.232483 2 TATCACG 100 4.4408377E-4 29.748432 2 TACACCG 80 0.00536505 29.74753 5 GTATAAC 460 0.0 24.61356 1 ATGGGAG 1550 0.0 21.11115 5 CGGGAGT 435 1.8189894E-12 20.51554 4 ATAGTAC 380 1.9463187E-10 20.35419 3 CGTGCCG 90 5.779391E-4 19.837095 10-11 TACAACG 180 5.787152E-4 19.832287 2 ATGGGAT 1650 0.0 19.831688 5 GTCTTCG 185 6.886099E-4 19.326721 1 >>END_MODULE