##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364159_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1353101 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.979660054940467 31.0 30.0 34.0 26.0 34.0 2 30.055659555347308 31.0 30.0 34.0 26.0 34.0 3 30.21250224484351 31.0 31.0 34.0 26.0 34.0 4 33.40707308619238 37.0 35.0 37.0 27.0 37.0 5 33.544505546888225 37.0 35.0 37.0 28.0 37.0 6 33.40450860652678 37.0 35.0 37.0 27.0 37.0 7 33.347507687896176 37.0 35.0 37.0 27.0 37.0 8 33.18478073698859 37.0 35.0 37.0 26.0 37.0 9 34.45438367128544 39.0 35.0 39.0 23.0 39.0 10-11 34.43182290161636 39.0 35.0 39.0 24.0 39.0 12-13 34.30659278206136 39.0 35.0 39.0 22.5 39.0 14-15 35.42272269401914 40.0 36.0 41.0 22.0 41.0 16-17 35.48762546180957 40.0 36.0 41.0 24.0 41.0 18-19 35.4414951285972 40.0 36.0 41.0 23.5 41.0 20-21 35.463284337237205 40.0 36.0 41.0 24.0 41.0 22-23 35.46791592054104 40.0 35.0 41.0 24.5 41.0 24-25 35.478731077724426 40.0 35.0 41.0 24.5 41.0 26-27 35.3234570072744 40.0 35.0 41.0 24.0 41.0 28-29 35.230956890875106 40.0 35.0 41.0 24.0 41.0 30-31 35.30937712705851 40.0 35.0 41.0 25.0 41.0 32-33 35.03566622151635 39.0 34.5 41.0 24.5 41.0 34-35 35.103305666021974 39.5 35.0 41.0 25.0 41.0 36-37 35.09968694132958 40.0 35.0 41.0 25.0 41.0 38-39 34.97792367310349 40.0 35.0 41.0 24.5 41.0 40-41 34.675574107180466 39.0 34.0 41.0 24.0 41.0 42-43 34.45606277727974 39.0 33.0 41.0 22.5 41.0 44-45 34.231192645633996 39.0 33.0 41.0 21.5 41.0 46-47 33.98786158609002 38.0 33.0 40.0 20.0 41.0 48-49 33.75213454132397 38.0 33.0 40.0 16.5 41.0 50-51 33.648451224261905 38.0 33.0 40.0 16.5 41.0 52-53 33.475460072825314 38.0 33.0 40.0 14.0 41.0 54-55 33.20304064515509 38.0 32.0 40.0 10.5 41.0 56-57 32.87263330675242 38.0 32.0 40.0 8.0 41.0 58-59 32.52058678546538 37.0 31.0 40.0 6.0 41.0 60-61 32.24642728074253 36.5 31.0 40.0 2.0 41.0 62-63 31.74441412725288 36.0 30.0 40.0 2.0 41.0 64-65 31.506795871113834 36.0 30.0 39.0 2.0 41.0 66-67 31.094844361211763 35.0 30.0 39.0 2.0 41.0 68-69 30.699675412256735 35.0 30.0 38.5 2.0 40.0 70-71 30.174324754767014 35.0 29.0 37.5 2.0 40.0 72-73 29.93564671077769 35.0 29.0 37.0 2.0 39.5 74-75 29.64752335561056 35.0 29.0 37.0 2.0 39.0 76-77 29.184333985415723 34.0 29.0 36.0 2.0 39.0 78-79 28.806763501024683 34.0 28.5 36.0 2.0 37.5 80-81 28.545426394629818 34.0 28.0 35.0 2.0 37.0 82-83 28.290145746695924 34.0 28.0 35.0 2.0 37.0 84-85 27.99853262986281 34.0 27.5 35.0 2.0 36.0 86-87 27.655134391298212 34.0 26.0 35.0 2.0 36.0 88-89 27.3554073199266 34.0 26.0 35.0 2.0 36.0 90-91 27.19149050957763 34.0 26.0 35.0 2.0 35.5 92-93 26.866393565594883 34.0 25.0 35.0 2.0 35.0 94-95 26.787513275062246 34.0 25.0 35.0 2.0 35.0 96-97 26.690267393195334 34.0 25.0 35.0 2.0 35.0 98-99 26.46818271511144 34.0 24.5 35.0 2.0 35.0 100-101 26.00298203903478 33.5 22.5 35.0 2.0 35.0 102-103 24.784604401297464 31.5 19.0 34.5 2.0 35.0 104-105 25.53508311648576 33.0 19.5 35.0 2.0 35.0 106-107 25.582997499817086 33.0 19.5 35.0 2.0 35.0 108-109 25.510064658883557 33.0 19.0 35.0 2.0 35.0 110-111 25.500173675135855 33.0 19.0 35.0 2.0 35.0 112-113 25.375748373550827 33.0 18.5 35.0 2.0 35.0 114-115 25.187521108919437 33.0 16.5 35.0 2.0 35.0 116-117 24.859128402092676 33.0 8.5 35.0 2.0 35.0 118-119 24.617715528995987 33.0 2.0 35.0 2.0 35.0 120-121 24.400948266241766 33.0 2.0 35.0 2.0 35.0 122-123 23.95696404037836 33.0 2.0 35.0 2.0 35.0 124-125 22.948633176680822 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6466005809709294 1101 2 0.6768533064948414 1101 3 0.6932421013296874 1101 4 0.7564395474326275 1101 5 0.7410428457243086 1101 6 0.6494358756874234 1101 7 0.7573786123930262 1101 8 0.7537598274924164 1101 9 0.8376948329358811 1101 10-11 0.776874619844186 1101 12-13 0.8063863800901885 1101 14-15 0.744603839081158 1101 16-17 0.8067220474227454 1101 18-19 0.8042657016926213 1101 20-21 0.8782677046530694 1101 22-23 0.9120352580058082 1101 24-25 0.9323761786744242 1101 26-27 0.9810585657791435 1101 28-29 1.074522694334732 1101 30-31 0.9741514966358267 1101 32-33 0.9873234668535034 1101 34-35 0.881023055401279 1101 36-37 0.9613246750824089 1101 38-39 1.0887697134408398 1101 40-41 1.133976858779235 1101 42-43 1.1097282345275374 1101 44-45 1.0254655907328356 1101 46-47 1.0914243504022991 1101 48-49 1.1517803499606885 1101 50-51 1.2500529053961884 1101 52-53 1.1561950097262965 1101 54-55 1.2341319503223147 1101 56-57 1.2670533271597293 1101 58-59 1.2634956846536696 1101 60-61 1.2240619328212219 1101 62-63 1.275472490765015 1101 64-65 1.1340428726515377 1101 66-67 1.118019956374738 1101 68-69 1.1800584437651054 1101 70-71 1.1480140656408437 1101 72-73 0.9892566923314092 1101 74-75 0.8935716615701566 1101 76-77 0.856736306557611 1101 78-79 0.866589369689688 1101 80-81 1.031193120124211 1101 82-83 0.9407897030700738 1101 84-85 0.9630109232360873 1101 86-87 0.9498958606679757 1101 88-89 0.9904326243222528 1101 90-91 1.0410394712041864 1101 92-93 0.9864811033366081 1101 94-95 0.9663886689438606 1101 96-97 0.9896290584710101 1101 98-99 1.0557087127208042 1101 100-101 0.8918056740520441 1101 102-103 0.8312009045698581 1101 104-105 0.7955698623496446 1101 106-107 0.7878186764498061 1101 108-109 0.8483619256583701 1101 110-111 0.8047420696283858 1101 112-113 0.7519852690861448 1101 114-115 0.817882178618941 1101 116-117 0.7390920537143835 1101 118-119 0.7329227604710873 1101 120-121 0.6360490838261939 1101 122-123 0.6705565655055175 1101 124-125 0.6815742591778822 1102 1 0.554524992541122 1102 2 0.6833585683053442 1102 3 0.644487686010855 1102 4 0.6367444063405259 1102 5 0.5729612960426422 1102 6 0.7462909800855186 1102 7 0.7990189928647453 1102 8 0.7824285421965342 1102 9 1.0184813768422032 1102 10-11 0.9139392214064301 1102 12-13 0.8658381338746466 1102 14-15 0.9615719969750955 1102 16-17 0.9526622398529341 1102 18-19 0.8728669561155584 1102 20-21 0.8395698541780874 1102 22-23 0.8894672262856105 1102 24-25 0.8489388434393419 1102 26-27 0.9896530086489648 1102 28-29 1.066111111951507 1102 30-31 1.0174818127711305 1102 32-33 0.9617001411698354 1102 34-35 0.8549905793788639 1102 36-37 0.9445704639577883 1102 38-39 0.9014565740005693 1102 40-41 1.0778101174445283 1102 42-43 1.0836367098063207 1102 44-45 1.06802446419033 1102 46-47 1.0476342163350907 1102 48-49 1.0126802826070787 1102 50-51 1.1058502685479752 1102 52-53 1.1578057058456892 1102 54-55 1.273461431394729 1102 56-57 1.3456970509306814 1102 58-59 1.2924817384938834 1102 60-61 1.2477522707778803 1102 62-63 1.3205159810840748 1102 64-65 1.2798108714568492 1102 66-67 1.3952284539015523 1102 68-69 1.334101641507388 1102 70-71 1.420289520582493 1102 72-73 1.422028415395296 1102 74-75 1.3748856055965604 1102 76-77 1.3105011754009261 1102 78-79 1.260544382962312 1102 80-81 1.409547086215781 1102 82-83 1.3014046781248574 1102 84-85 1.2910214925722165 1102 86-87 1.222736278321328 1102 88-89 1.2432178181086542 1102 90-91 1.1741984775403012 1102 92-93 1.1160815684117793 1102 94-95 1.0994051964841738 1102 96-97 1.2528921581391188 1102 98-99 1.165264239831398 1102 100-101 1.3024230872466447 1102 102-103 1.1375775545047908 1102 104-105 1.1362568539593951 1102 106-107 1.1892294451038623 1102 108-109 1.1606773508547157 1102 110-111 1.258490938179122 1102 112-113 1.3400604034124086 1102 114-115 1.2985046814304226 1102 116-117 1.4056672901377532 1102 118-119 1.3702666052347254 1102 120-121 1.3267234275856232 1102 122-123 1.1289699003017368 1102 124-125 0.8072474143441504 1103 1 0.36195095112834963 1103 2 0.36391278246366454 1103 3 0.46350034500357395 1103 4 0.5964955552782172 1103 5 0.5409728104314198 1103 6 0.52697401790698 1103 7 0.5233710237071421 1103 8 0.6746857001811009 1103 9 0.7424906703724474 1103 10-11 0.6400137301207209 1103 12-13 0.5610987256710374 1103 14-15 0.5449601831369293 1103 16-17 0.5329895638917392 1103 18-19 0.5946071008081617 1103 20-21 0.5276228090739536 1103 22-23 0.6006932105286111 1103 24-25 0.6208636304295894 1103 26-27 0.8722449249021835 1103 28-29 0.9184984358219239 1103 30-31 0.8240552618228847 1103 32-33 0.7978525136599117 1103 34-35 0.750767604983615 1103 36-37 0.7883321495913975 1103 38-39 0.7782926578998968 1103 40-41 0.8156320465900322 1103 42-43 0.8588089575935172 1103 44-45 0.7840586765061914 1103 46-47 0.8233734417784788 1103 48-49 0.7887123453854556 1103 50-51 0.854152926396587 1103 52-53 0.8289278337491126 1103 54-55 0.9290910403324517 1103 56-57 0.8738250365713753 1103 58-59 0.8636071600434505 1103 60-61 0.9650139234750341 1103 62-63 0.8650557348976413 1103 64-65 0.9116359625026469 1103 66-67 0.907922488043539 1103 68-69 0.9199435940402516 1103 70-71 0.7917541133912493 1103 72-73 0.8503092707923443 1103 74-75 0.75683055564458 1103 76-77 0.808997365915328 1103 78-79 0.8333432491555826 1103 80-81 0.7945112944356296 1103 82-83 0.8294430171564429 1103 84-85 0.7890614016576762 1103 86-87 0.7395216153059039 1103 88-89 0.7538032996174309 1103 90-91 0.882890706863261 1103 92-93 0.7727957279698643 1103 94-95 0.8515149873302157 1103 96-97 0.7539845095003379 1103 98-99 0.857249387220822 1103 100-101 0.591239326061249 1103 102-103 0.5200362390571946 1103 104-105 0.5973325191644747 1103 106-107 0.7468363065859833 1103 108-109 0.6802657540504953 1103 110-111 0.49746935811058535 1103 112-113 0.6237057416153853 1103 114-115 0.4208159641743414 1103 116-117 0.35692974717796133 1103 118-119 0.41608518526215477 1103 120-121 0.48199259276767137 1103 122-123 0.4183334291884968 1103 124-125 0.31007474952687986 1104 1 0.27141710910603223 1104 2 0.3635544823639627 1104 3 0.32674880526794325 1104 4 0.33789384026197666 1104 5 0.3438499652572631 1104 6 0.2998951737941553 1104 7 0.31330867044434996 1104 8 0.2967695494397731 1104 9 0.44838608895540943 1104 10-11 0.29139878865479574 1104 12-13 0.240556571890437 1104 14-15 0.42956770130822264 1104 16-17 0.3725183565460384 1104 18-19 0.3182162632402168 1104 20-21 0.3059287682126737 1104 22-23 0.37347215193020844 1104 24-25 0.4700471785754772 1104 26-27 0.4718968260535732 1104 28-29 0.4730653298977643 1104 30-31 0.43648410432762574 1104 32-33 0.45355124408154524 1104 34-35 0.48740220722527283 1104 36-37 0.5793625362539885 1104 38-39 0.5560189548155989 1104 40-41 0.5682733163291971 1104 42-43 0.5398125697409384 1104 44-45 0.5344036597017663 1104 46-47 0.6621110093178046 1104 48-49 0.5246250709185318 1104 50-51 0.4575034092222978 1104 52-53 0.6491189740379255 1104 54-55 0.6889349963675713 1104 56-57 0.6506234216580538 1104 58-59 0.6844136418078293 1104 60-61 0.7572553946260925 1104 62-63 0.6793909550436545 1104 64-65 0.7841522342519092 1104 66-67 0.7172663817655973 1104 68-69 0.7647446244168634 1104 70-71 0.5371228459033901 1104 72-73 0.5354220272809442 1104 74-75 0.5033249434489093 1104 76-77 0.4811173466987846 1104 78-79 0.5411824332163064 1104 80-81 0.39315940253069925 1104 82-83 0.5005767932475251 1104 84-85 0.5307589400907631 1104 86-87 0.42845129803191995 1104 88-89 0.43984798813253434 1104 90-91 0.37883282003986096 1104 92-93 0.19674037588097093 1104 94-95 0.32339244970117065 1104 96-97 0.43726330215462994 1104 98-99 0.44183710591956427 1104 100-101 0.13804347008080597 1104 102-103 -0.08677789515308021 1104 104-105 0.09137031505101234 1104 106-107 0.25354551364223354 1104 108-109 0.24078505300845876 1104 110-111 0.16519677063109128 1104 112-113 0.3381727237335461 1104 114-115 0.47792839703704004 1104 116-117 0.3574056380537236 1104 118-119 0.3628105334112597 1104 120-121 0.40442564642926726 1104 122-123 0.3807292558215032 1104 124-125 0.3818425610117764 1105 1 0.1911280903693111 1105 2 0.2735158097092736 1105 3 0.20219046227650495 1105 4 0.07753049437310722 1105 5 0.20373402777738647 1105 6 0.21253360660595177 1105 7 0.24729754752888766 1105 8 0.24092659824538742 1105 9 0.2771257869787149 1105 10-11 0.2631015524819773 1105 12-13 0.40602452710903947 1105 14-15 0.29743529273826397 1105 16-17 0.4080179219313962 1105 18-19 0.40778259428780217 1105 20-21 0.2841170489093443 1105 22-23 0.29440548687322377 1105 24-25 0.2774268748281301 1105 26-27 0.41963693052205286 1105 28-29 0.5209871274317308 1105 30-31 0.31972568568104975 1105 32-33 0.18770491255106236 1105 34-35 0.36841789761868426 1105 36-37 0.30846934280218363 1105 38-39 0.3202545964355821 1105 40-41 0.30219257458688276 1105 42-43 0.26708836090055854 1105 44-45 0.3125555213011495 1105 46-47 0.23641351318104142 1105 48-49 0.3399530842467158 1105 50-51 0.44277329284216194 1105 52-53 0.24569698736610945 1105 54-55 0.4292738750617957 1105 56-57 0.39159259218796194 1105 58-59 0.3944990154154553 1105 60-61 0.37395998946866627 1105 62-63 0.4637738022524651 1105 64-65 0.35922135005256806 1105 66-67 0.21114505586900378 1105 68-69 0.246226720708151 1105 70-71 0.19573304486031518 1105 72-73 0.1458438248149072 1105 74-75 0.17497358165636712 1105 76-77 0.17158972854798904 1105 78-79 0.06934184020877154 1105 80-81 0.23954022958626808 1105 82-83 0.08688401750868024 1105 84-85 0.21232977020472887 1105 86-87 0.12971107686135497 1105 88-89 0.07784216107696551 1105 90-91 0.05520286221358006 1105 92-93 0.18692683763488205 1105 94-95 0.12468251070818681 1105 96-97 0.11953182961583764 1105 98-99 0.032821970867090045 1105 100-101 0.08195604815047375 1105 102-103 -0.15744603468047558 1105 104-105 -0.007971827277234667 1105 106-107 -0.032930928634502976 1105 108-109 0.04805609560069257 1105 110-111 0.06940599331475283 1105 112-113 -0.027174203379967565 1105 114-115 0.19469144352471446 1105 116-117 0.06802774062277805 1105 118-119 0.05591022113258859 1105 120-121 -0.06846979253152341 1105 122-123 -0.10120053379281657 1105 124-125 -0.0793333734096997 1106 1 0.15937188311248462 1106 2 0.24037450411359984 1106 3 0.21311255236409465 1106 4 0.1876934454164001 1106 5 0.18888745219968683 1106 6 0.1820563783654805 1106 7 0.2048182239141596 1106 8 0.15896654541726463 1106 9 0.25825849497982034 1106 10-11 0.4129514418767073 1106 12-13 0.2976689810720643 1106 14-15 0.35658716781845357 1106 16-17 0.3327526718612148 1106 18-19 0.3151164283113914 1106 20-21 0.24369077241448167 1106 22-23 0.3441532605380715 1106 24-25 0.3944444105638141 1106 26-27 0.45324166402266997 1106 28-29 0.6076230629073223 1106 30-31 0.46726880630302503 1106 32-33 0.24296003787316067 1106 34-35 0.4543478984193996 1106 36-37 0.4592212464381191 1106 38-39 0.38069027413769163 1106 40-41 0.3324904852903643 1106 42-43 0.40458198168219184 1106 44-45 0.3520181619936622 1106 46-47 0.41396428404144814 1106 48-49 0.44947943994517914 1106 50-51 0.4225438146308633 1106 52-53 0.4279033817305944 1106 54-55 0.31669779474297854 1106 56-57 0.37547089647836174 1106 58-59 0.45636730397125547 1106 60-61 0.45480156875571964 1106 62-63 0.39505732028165497 1106 64-65 0.2691636693771855 1106 66-67 0.32407627414776385 1106 68-69 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78-79 -3.7522570926760643 2216 80-81 -3.966894962982593 2216 82-83 -3.963606406002647 2216 84-85 -3.8940440439103803 2216 86-87 -4.024104173992868 2216 88-89 -4.163563647161279 2216 90-91 -4.271064095396625 2216 92-93 -4.434961951373058 2216 94-95 -4.386960063707146 2216 96-97 -4.304494814432626 2216 98-99 -4.17241162972833 2216 100-101 -4.378707974893032 2216 102-103 -4.0491015855641805 2216 104-105 -4.171005324447545 2216 106-107 -4.144917004908105 2216 108-109 -4.02463145907204 2216 110-111 -4.10684864027521 2216 112-113 -4.231917863758966 2216 114-115 -4.2510019155199075 2216 116-117 -4.281096649821517 2216 118-119 -4.244896156427064 2216 120-121 -4.1780629548751165 2216 122-123 -4.185794662726234 2216 124-125 -4.241283081741223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52670.0 3 8635.0 4 6572.0 5 4630.0 6 5065.0 7 4854.0 8 5239.0 9 5921.0 10 7385.0 11 8300.0 12 9139.0 13 9948.0 14 10313.0 15 10483.0 16 10225.0 17 10155.0 18 10226.0 19 10548.0 20 10800.0 21 11721.0 22 13151.0 23 16841.0 24 19834.0 25 20217.0 26 23806.0 27 24568.0 28 27594.0 29 34089.0 30 39677.0 31 46053.0 32 57797.0 33 72493.0 34 89681.0 35 114982.0 36 164833.0 37 260111.0 38 113816.0 39 729.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.23265277631322 17.298806958345274 9.909612205768125 22.55892805957337 2 13.779116260110083 23.890944379970584 36.852566715597504 25.477372644321832 3 22.06348416830424 32.40822471646524 26.16723075426889 19.361060360961623 4 13.269745301424496 19.578783730412084 32.66523336233597 34.486237605827455 5 11.632649879392664 42.47590637396218 31.668019121960782 14.223424624684375 6 29.7842516072175 31.15221476390478 21.17376807214827 17.88976555672945 7 25.728235339531373 34.724651322997985 21.2950563138969 18.25205702357374 8 28.849092553458483 33.066146103274114 21.482447321172664 16.60231402209474 9 31.659072141062122 17.401365232321194 18.30194031712838 32.63762230948831 10-11 23.371482615965665 31.328580547393496 23.709059393238142 21.590877443402697 12-13 26.835056480843004 28.621663942975907 23.400606824191424 21.142672751989668 14-15 27.190427098619846 27.30751568389349 22.59226851523581 22.909788702250854 16-17 22.792769414166028 30.487378847666687 27.70123250815912 19.01861923000817 18-19 24.537877387450994 29.350752900353456 27.785189359097423 18.326180353098128 20-21 22.480792532623738 29.72027037461615 27.620701892978694 20.17823519978142 22-23 21.124215360189865 31.631092863184364 22.589235257905436 24.65545651872034 24-25 21.461317913289726 32.77167636173694 22.0648142270098 23.702191497963536 26-27 23.458677715100844 28.494334014145196 23.113234692964493 24.93375357778947 28-29 23.09491827222592 28.504329385242887 25.77582539000109 22.624926952530096 30-31 25.49877485426911 30.123583953208676 24.232227774994996 20.145413417527212 32-33 19.83162611536652 30.728289641994778 26.643628166478724 22.79645607615998 34-35 18.214592555134026 31.761533794766805 27.372457901806897 22.651415748292273 36-37 23.839974465984646 29.639090796063755 24.864452605267502 21.656482132684097 38-39 25.967670908232225 27.92937179345917 24.178518972779113 21.92443832552949 40-41 21.714707771021608 31.562562253236052 24.253693831119328 22.469036144623008 42-43 24.66001184245088 29.78743691075274 23.72344909155936 21.82910215523702 44-45 26.06258672653109 28.096556991508358 23.965080405398634 21.875775876561914 46-47 22.510845968870054 29.525902463665215 28.570224980783642 19.393026586681085 48-49 24.34642706036921 28.98167323579843 27.455407842875275 19.21649186095709 50-51 20.924515068411814 30.08828408251723 27.787888121541837 21.199312727529122 52-53 20.047645729510297 31.559618388876725 23.292230851723076 25.100505029889902 54-55 20.841112027670444 32.382597840853386 22.49359057851221 24.282699552963958 56-57 23.075581708124716 28.564517056574513 23.187665914120718 25.172235321180057 58-59 22.78289345288405 28.288009581471073 25.742189268188433 23.186907697456444 60-61 24.700585692827413 29.972600381160465 24.44607054437076 20.880743381641366 62-63 19.538530214912477 30.562345101563647 26.627075996201732 23.27204868732214 64-65 17.78299238208411 31.335512284153793 27.42112003812112 23.460375295640972 66-67 23.031996728560305 29.64267239811818 24.971482515832122 22.35384835748939 68-69 25.03562427081571 28.085432938794536 24.288145430148226 22.59079736024153 70-71 21.00599665541165 30.967726132695393 24.529504895524294 23.496772316368663 72-73 23.85513507094054 29.4993894993895 24.03320084171148 22.612274587958478 74-75 25.149161001839364 27.76729102243019 24.273457135604016 22.810090840126428 76-77 21.741997638276253 29.09818818232626 28.733093301447838 20.42672087794965 78-79 23.469229479021482 28.73144796077855 27.56741372067928 20.231908839520685 80-81 20.22990169336792 29.82435436404998 27.87299183932488 22.07275210325722 82-83 19.40564702127699 31.081402533496373 23.539213157704303 25.973737287522336 84-85 20.21885035836774 31.760095119415094 22.92273254566897 25.098321976548192 86-87 22.101412163937493 28.00346472262179 23.912078581215386 25.983044532225335 88-89 22.02613845514457 27.728470284391143 26.168252389716795 24.07713887074749 90-91 23.623700180866987 29.36761864896738 24.976417527399086 22.032263642766544 92-93 19.255031808556357 29.784179817246464 26.784058832654555 24.17672954154262 94-95 17.68760776030027 30.395511382233902 27.690621152080542 24.226259705385285 96-97 22.48814744764342 28.690318470912228 25.714890975810366 23.106643105633985 98-99 23.987735021875782 27.47758640897404 24.90994506203702 23.624733507113152 100-101 20.483180264804478 29.819655823910736 25.177195274199253 24.519968637085533 102-103 22.905103756089478 28.620616384484364 24.951226720748362 23.523053138677792 104-105 23.908308886053238 27.175585427529807 25.05133279858964 23.864772887827314 106-107 20.84822220062713 28.43826198422454 29.012390554307245 21.701125260841085 108-109 22.292110474792935 28.493251396291612 27.76232421859786 21.452313910317592 110-111 19.416058394160586 29.590851334180435 28.160120201448724 22.83297007021026 112-113 18.719625932460687 30.59828844216184 24.29012789940183 26.391957725975644 114-115 19.223090055579423 31.90196475592465 23.568316094141494 25.30662909435443 116-117 20.941793612139886 28.48727627691557 24.387327532301647 26.183602578642894 118-119 20.705085666694874 28.382984535309586 26.171060230196304 24.74086956779924 120-121 21.9553140845222 29.829075278363703 25.336246723086063 22.87936391402803 122-123 18.494814579575447 30.312282735076472 26.578423872596773 24.614478812751305 124-125 17.387362321421286 30.995069724995272 27.230328054340607 24.38723989924284 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9696.0 1 6471.0 2 4795.5 3 6186.0 4 5247.0 5 3861.5 6 2830.0 7 2373.0 8 2326.5 9 2164.5 10 1818.0 11 1519.0 12 1385.5 13 1355.0 14 1385.5 15 1406.0 16 1418.0 17 1518.0 18 1696.5 19 1869.5 20 2130.0 21 2513.5 22 2972.5 23 3616.0 24 4373.0 25 5196.0 26 6304.0 27 7583.0 28 8779.5 29 10155.0 30 12131.5 31 14024.5 32 15736.5 33 17782.5 34 20095.5 35 21618.5 36 23314.5 37 25400.0 38 25577.0 39 25223.0 40 24994.0 41 25636.0 42 26559.5 43 27598.5 44 29295.5 45 30904.5 46 33318.5 47 38753.0 48 48699.0 49 76618.5 50 166955.0 51 175898.5 52 81893.0 53 40760.5 54 31350.5 55 26570.5 56 22807.0 57 19622.0 58 18204.5 59 16842.0 60 14980.0 61 13358.5 62 11769.0 63 9152.0 64 6481.0 65 5181.5 66 3740.0 67 2714.5 68 2082.5 69 1476.0 70 1236.5 71 1159.0 72 1178.5 73 1183.5 74 875.5 75 638.5 76 478.0 77 308.5 78 269.0 79 166.0 80 89.5 81 73.0 82 52.5 83 37.0 84 26.0 85 23.0 86 19.5 87 15.0 88 12.5 89 8.5 90 5.0 91 4.0 92 3.0 93 3.5 94 5.0 95 4.5 96 3.5 97 3.0 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47734795850420625 2 0.46574498134285613 3 0.44992945833311776 4 0.4505945971512843 5 0.45584180338348723 6 0.45879797590867205 7 0.4440910175958779 8 0.469661909938726 9 0.48163440866572416 10-11 0.48721418430701036 12-13 0.48647514117571417 14-15 0.46245623940858815 16-17 0.46519069899438403 18-19 0.4515553532219694 20-21 0.46034996648439397 22-23 0.4777174800698543 24-25 0.46430384723682855 26-27 0.47479825970123446 28-29 0.47106609188818865 30-31 0.48233649964045555 32-33 0.4814496478829001 34-35 0.47505692479718803 36-37 0.4344465047324627 38-39 0.39668140072322766 40-41 0.3901778211678212 42-43 0.3996006210918475 44-45 0.4311947149547595 46-47 0.4391024764596287 48-49 0.4353703086465829 50-51 0.4246541832427882 52-53 0.41800279506112253 54-55 0.41907440760150205 56-57 0.4030371716523748 58-59 0.40647372221290207 60-61 0.41659861311165985 62-63 0.41722679977326155 64-65 0.4298644373184264 66-67 0.41992430720249263 68-69 0.4216241064044739 70-71 0.4323402318082686 72-73 0.4320446145557501 74-75 0.43596154315161983 76-77 0.42716692988919525 78-79 0.4194069770105853 80-81 0.42761035576797296 82-83 0.42520846559126035 84-85 0.4243585659902698 86-87 0.429162346343695 88-89 0.41837231662677066 90-91 0.42140239346508507 92-93 0.43027091104063925 94-95 0.42731473851545454 96-97 0.39875072149085694 98-99 0.37210821660762944 100-101 0.3704084174056482 102-103 0.3525605257848453 104-105 0.35411251636056734 106-107 0.37380801580961065 108-109 0.38101368633974847 110-111 0.39638578347070913 112-113 0.39339265878895957 114-115 0.3916928595869783 116-117 0.36582634999161184 118-119 0.43625716040413837 120-121 0.4425020748635911 122-123 0.3976052046373479 124-125 0.392136285465756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1353101.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.07478770702152 #Duplication Level Percentage of deduplicated Percentage of total 1 85.47187502593201 43.654578718705515 2 9.527603848283029 9.732406878153139 3 2.3851320840374273 3.6546034453625227 4 0.9018950413589777 1.8425639108570073 5 0.46830077975374906 1.195918145447769 6 0.26937400759997204 0.8254932151174905 7 0.1699151702822161 0.607486687525662 8 0.12677068670374744 0.5179828728693788 9 0.0984998396749042 0.4527772560514249 >10 0.5176998505981407 4.913800059638972 >50 0.03772622387369194 1.2988992925225837 >100 0.01815640927023121 1.632379247018595 >500 0.0019390339737736062 0.712517194691578 >1k 0.0017627581579760057 2.827470621593254 >5k 0.0017627581579760057 6.1493997260328745 >10k+ 0.0015864823421784051 19.981722728412198 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 61126 4.517475044361064 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 43921 3.2459513369659763 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 28833 2.130883060466292 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 16524 1.221194870153817 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 16217 1.1985062460230242 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 15716 1.161480185145085 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 15082 1.114624850620907 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 13009 0.9614212095032079 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 11511 0.8507125484350392 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 9817 0.7255186419934654 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 9210 0.6806587239237869 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 6769 0.5002582955743881 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 6765 0.4999626783218696 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6394 0.47254417815078104 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 6392 0.47239636952452185 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 6122 0.4524422049795248 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 5823 0.43034481535376884 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 5623 0.4155639527278452 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5387 0.39812253482925514 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4940 0.36508730686031565 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 4343 0.32096643192193336 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 3852 0.2846794141752907 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 3796 0.280540772640032 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 3072 0.22703404993418821 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 2900 0.21432250807589381 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2642 0.19525519528845223 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 2392 0.1767791170060476 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1818 0.13435804126964654 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 1650 0.12194211666387061 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.4780862625923713E-4 0.0 0.0 0.0 0.0 8 1.4780862625923713E-4 0.0 0.0 0.0 0.0 9 1.4780862625923713E-4 0.0 0.0 0.0 0.0 10-11 1.4780862625923713E-4 0.0 0.0 2.9561725251847426E-4 0.0 12-13 2.2171293938885568E-4 0.0 0.0 7.759952878609948E-4 0.0 14-15 2.2171293938885568E-4 0.0 0.0 0.0013302776363331341 0.0 16-17 2.2171293938885568E-4 0.0 0.0 0.002106272924194129 0.0 18-19 4.064737222129021E-4 0.0 0.0 0.003473502717092072 0.0 20-21 5.173301919073299E-4 0.0 0.0 0.0053211105453325365 0.0 22-23 5.173301919073299E-4 0.0 0.0 0.008646804636165371 0.0 24-25 5.173301919073299E-4 0.0 0.0 0.012452876762340727 0.0 26-27 5.173301919073299E-4 0.0 0.0 0.01747837005515479 0.0 28-29 5.912345050369485E-4 0.0 0.0 0.024979657837811073 0.0 30-31 5.912345050369485E-4 0.0 0.0 0.04005613771625326 0.0 32-33 5.912345050369485E-4 0.0 0.0 0.06880491552367488 0.0 34-35 5.912345050369485E-4 0.0 0.0 0.11240846027014983 0.0 36-37 6.651388181665671E-4 0.0 0.0 0.17101458058193736 0.0 38-39 6.651388181665671E-4 0.0 0.0 0.2594041390849611 0.0 40-41 6.651388181665671E-4 0.0 0.0 0.3926166635010986 0.0 42-43 7.020909747313764E-4 0.0 0.0 0.49238748622608364 0.0 44-45 7.390431312961856E-4 0.0 0.0 0.6015811088750951 0.0 46-47 7.759952878609948E-4 0.0 0.0 0.7471356535838788 0.0 48-49 8.129474444258042E-4 0.0 0.0 0.8774289576313963 0.0 50-51 8.129474444258042E-4 0.0 0.0 1.0079809267748676 0.0 52-53 8.129474444258042E-4 0.0 0.0 1.1733048752458242 0.0 54-55 8.129474444258042E-4 0.0 0.0 1.347497341292335 0.0 56-57 8.868517575554227E-4 0.0 0.0 1.5317777460810391 0.0 58-59 8.868517575554227E-4 0.0 0.0 1.7487238572730344 0.0 60-61 8.868517575554227E-4 0.0 0.0 1.9504456799603282 0.0 62-63 8.868517575554227E-4 0.0 0.0 2.1514284595163256 0.0 64-65 8.868517575554227E-4 0.0 0.0 2.388919969758355 0.0 66-67 9.607560706850413E-4 0.0 0.0 2.6493957213836956 0.0 68-69 9.607560706850413E-4 0.0 0.0 2.969401397234944 0.0 70-71 9.977082272498504E-4 0.0 0.0 3.4211045590831723 0.0 72-73 0.0010346603838146597 0.0 0.0 3.78874156474646 0.0 74-75 0.001071612540379469 0.0 0.0 4.130844630223464 0.0 76-77 0.0011085646969442784 0.0 0.0 4.533031902274849 0.0 78-79 0.0011085646969442784 0.0 0.0 4.932558619053567 0.0 80-81 0.0011085646969442784 0.0 0.0 5.362127439119474 0.0 82-83 0.0011085646969442784 0.0 0.0 5.884224459223665 0.0 84-85 0.0011085646969442784 0.0 0.0 6.3852957022424786 0.0 86-87 0.0011085646969442784 0.0 0.0 6.881710973534126 0.0 88-89 0.0011085646969442784 0.0 0.0 7.454025974409893 0.0 90-91 0.0011085646969442784 0.0 0.0 8.00967555267493 0.0 92-93 0.0011085646969442784 0.0 0.0 8.567874829742939 0.0 94-95 0.0011085646969442784 0.0 0.0 9.17019498174933 0.0 96-97 0.0011085646969442784 3.695215656480928E-5 0.0 9.770482765144656 0.0 98-99 0.001182469010073897 7.390431312961856E-5 0.0 10.462485801133841 0.0 100-101 0.0012194211666387064 7.390431312961856E-5 0.0 11.419029325970493 0.0 102-103 0.0012563733232035155 7.390431312961856E-5 0.0 12.217011146987549 0.0 104-105 0.0012563733232035155 7.390431312961856E-5 0.0 12.953172010071679 0.0 106-107 0.0012933254797683248 7.390431312961856E-5 0.0 13.739403045301128 0.0 108-109 0.0013302776363331341 7.390431312961856E-5 0.0 14.444967522749597 0.0 110-111 0.0013302776363331341 7.390431312961856E-5 0.0 15.186745113631577 0.0 112-113 0.0013302776363331341 7.390431312961856E-5 0.0 16.071934024141584 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 85 9.786163E-10 56.02804 2 ATCACGC 135 2.7284841E-11 44.09942 3 TATAACG 150 8.367351E-11 39.686535 2 AGTACTC 1320 0.0 36.53236 5 ATAACGC 175 4.4383341E-10 34.01955 3 AAGTACT 1470 0.0 33.61456 4 TCACGCA 185 8.039933E-10 32.18066 4 GAACAAA 1655 0.0 32.013 1 ATGGGGG 2085 0.0 28.553581 5 CATGGGG 5720 0.0 27.581413 4 GTACAAA 1350 0.0 26.016727 1 ATGGGAG 2835 0.0 24.779675 5 TAACGCA 265 1.369699E-9 24.712318 4 AAAGTAC 1880 0.0 23.750355 3 CGTTTAA 185 2.6805421E-5 22.524788 1 GTACATA 840 0.0 21.96933 1 GTATAGG 275 5.487891E-8 21.647198 1 ACTTGAC 445 0.0 21.399998 8 CGGTTAC 225 0.0 21.163008 28-29 AAAAAGT 2110 0.0 21.15988 1 >>END_MODULE