##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364159_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1353101 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0643802642966 33.0 31.0 34.0 30.0 34.0 2 31.85324598828912 33.0 31.0 34.0 30.0 34.0 3 32.08735489811921 34.0 31.0 34.0 30.0 34.0 4 35.56230244453297 37.0 35.0 37.0 33.0 37.0 5 35.71121889644601 37.0 35.0 37.0 33.0 37.0 6 35.863424829336466 37.0 35.0 37.0 35.0 37.0 7 35.8397096742963 37.0 35.0 37.0 35.0 37.0 8 35.88247366604563 37.0 35.0 37.0 35.0 37.0 9 37.533731037077054 39.0 37.0 39.0 35.0 39.0 10-11 37.49704013225916 39.0 37.0 39.0 34.5 39.0 12-13 37.473852654014735 39.0 37.0 39.0 34.0 39.0 14-15 38.719977296595005 40.0 38.0 41.0 34.0 41.0 16-17 38.713142625716785 40.0 38.0 41.0 34.0 41.0 18-19 38.80182780147232 40.0 38.0 41.0 34.5 41.0 20-21 38.832993989362215 40.0 38.0 41.0 35.0 41.0 22-23 38.74660760726657 40.0 38.0 41.0 34.5 41.0 24-25 38.69301367747123 40.0 38.0 41.0 34.5 41.0 26-27 38.48282426810711 40.0 38.0 41.0 34.0 41.0 28-29 38.30244601105165 40.0 38.0 41.0 34.0 41.0 30-31 38.097838224936645 40.0 37.0 41.0 34.0 41.0 32-33 37.70266890646005 40.0 36.5 41.0 33.0 41.0 34-35 37.40244076384542 40.0 36.0 41.0 33.0 41.0 36-37 37.23232301210331 40.0 36.0 41.0 33.0 41.0 38-39 37.115845010830675 40.0 35.5 41.0 32.5 41.0 40-41 36.86506144035072 40.0 35.0 41.0 31.0 41.0 42-43 36.65312641111048 40.0 35.0 41.0 31.0 41.0 44-45 36.3412576001348 40.0 35.0 41.0 30.0 41.0 46-47 35.899022319841606 39.0 35.0 41.0 29.0 41.0 48-49 35.46391437150663 39.0 35.0 41.0 26.5 41.0 50-51 35.280345295731806 39.0 35.0 41.0 26.0 41.0 52-53 35.17822357680616 39.0 35.0 41.0 25.5 41.0 54-55 35.00121240025689 39.0 35.0 41.0 24.5 41.0 56-57 34.737926067603226 39.0 35.0 41.0 23.0 41.0 58-59 34.566653560968476 38.0 35.0 40.0 23.0 41.0 60-61 34.3835227377705 38.0 34.5 40.0 23.0 41.0 62-63 34.05023867397925 38.0 34.0 40.0 22.0 41.0 64-65 33.77579537669398 37.0 34.0 40.0 22.0 41.0 66-67 33.328081569668484 36.5 34.0 39.0 21.5 41.0 68-69 33.032940999969696 36.0 33.5 39.0 20.0 41.0 70-71 32.58293098593527 35.0 33.0 39.0 18.5 40.5 72-73 32.27443590685396 35.0 33.0 37.5 18.0 39.5 74-75 31.96382383872305 35.0 33.0 37.0 17.5 39.0 76-77 31.49145111857873 35.0 32.5 36.5 12.5 39.0 78-79 31.088105026897473 35.0 32.0 36.0 9.5 37.5 80-81 30.887876440856964 35.0 32.0 36.0 7.0 37.0 82-83 30.6788761518911 35.0 32.0 35.0 6.5 37.0 84-85 30.44610564917179 35.0 32.0 35.0 2.0 36.0 86-87 30.205329461732717 35.0 32.0 35.0 2.0 36.0 88-89 30.002918481325487 35.0 31.0 35.0 2.0 36.0 90-91 29.837136695634694 35.0 31.0 35.0 2.0 35.5 92-93 29.65480736471261 35.0 31.0 35.0 2.0 35.0 94-95 29.5693285275822 34.5 31.0 35.0 2.0 35.0 96-97 29.471760422910044 35.0 31.0 35.0 2.0 35.0 98-99 29.376070596356072 35.0 31.0 35.0 2.0 35.0 100-101 29.13967065281897 34.0 30.5 35.0 2.0 35.0 102-103 27.786486744152874 32.5 28.0 34.5 2.0 35.0 104-105 28.677487489847394 34.0 29.5 35.0 2.0 35.0 106-107 28.708050249020584 34.0 29.5 35.0 2.0 35.0 108-109 28.619847298908212 34.0 29.0 35.0 2.0 35.0 110-111 28.634370235481313 34.0 30.0 35.0 2.0 35.0 112-113 28.544358477305096 34.0 30.0 35.0 2.0 35.0 114-115 28.42391809628402 34.0 30.0 35.0 2.0 35.0 116-117 28.21004418738882 34.0 29.0 35.0 2.0 35.0 118-119 28.00374362298158 34.0 29.0 35.0 2.0 35.0 120-121 27.79644793699805 34.0 29.0 35.0 2.0 35.0 122-123 27.489468265857465 34.0 29.0 35.0 2.0 35.0 124-125 26.467679426739025 33.0 26.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23852383557496637 1101 2 0.5728770509925418 1101 3 0.4502773378372922 1101 4 0.45646452323655495 1101 5 0.17581322944280942 1101 6 0.2189842094408192 1101 7 0.22222607846518372 1101 8 0.21464234753234024 1101 9 0.2948596238231431 1101 10-11 0.3016019889163104 1101 12-13 0.24652614694871744 1101 14-15 0.3710237441224109 1101 16-17 0.36122894511358083 1101 18-19 0.21567536172494073 1101 20-21 0.15951849760160997 1101 22-23 0.19703389443224495 1101 24-25 0.2598271100249505 1101 26-27 0.1790509413759125 1101 28-29 0.23716433782215063 1101 30-31 0.25706153143777755 1101 32-33 0.1333277597449083 1101 34-35 0.2498558970049416 1101 36-37 0.1892098393038424 1101 38-39 0.16795382506176537 1101 40-41 -0.0014261260272405707 1101 42-43 -0.04448759763742771 1101 44-45 0.05931585834539277 1101 46-47 0.18775488085003644 1101 48-49 0.14404612691279794 1101 50-51 0.07490551815966029 1101 52-53 0.2150783597405166 1101 54-55 0.27055409416352205 1101 56-57 0.23502969357591752 1101 58-59 0.1909998808621438 1101 60-61 0.1964137415051539 1101 62-63 0.23981640289660788 1101 64-65 0.22961108687736242 1101 66-67 0.28273467178877354 1101 68-69 0.29319400900197223 1101 70-71 0.1806886366100855 1101 72-73 0.08790578517042746 1101 74-75 0.1476966940134119 1101 76-77 0.11175461513760254 1101 78-79 0.11041352902186219 1101 80-81 0.21411196111450792 1101 82-83 0.22398062881284275 1101 84-85 0.19719950667744612 1101 86-87 0.2174377967487402 1101 88-89 0.20950175562555629 1101 90-91 0.1616918040489459 1101 92-93 0.2837369296204031 1101 94-95 0.3847214137182071 1101 96-97 0.3662044422132631 1101 98-99 0.2287100385147376 1101 100-101 0.07059909802527287 1101 102-103 -0.07245912625137763 1101 104-105 0.19949009909941395 1101 106-107 0.2623136448261789 1101 108-109 0.09437486601881773 1101 110-111 -0.016272906280910604 1101 112-113 0.1894832434861229 1101 114-115 0.18811792591995413 1101 116-117 0.18017998868079488 1101 118-119 0.2012486121893673 1101 120-121 0.19098749662155257 1101 122-123 0.21787032228424863 1101 124-125 0.350124977882448 1102 1 0.3512093246810508 1102 2 0.7179859901274277 1102 3 0.5493633401718085 1102 4 0.49790832868518464 1102 5 0.32995852429010597 1102 6 0.28721402850036526 1102 7 0.2631098736736348 1102 8 0.2685237137439884 1102 9 0.27016812470240126 1102 10-11 0.25854253487676715 1102 12-13 0.3148458724141676 1102 14-15 0.4745147793870572 1102 16-17 0.5288038188636506 1102 18-19 0.4077398261418139 1102 20-21 0.390543674442938 1102 22-23 0.2795877849414907 1102 24-25 0.3303354469393085 1102 26-27 0.4056196557752614 1102 28-29 0.5255947548354882 1102 30-31 0.5164357980910452 1102 32-33 0.49412330953020245 1102 34-35 0.42769533940236926 1102 36-37 0.45565394951995586 1102 38-39 0.4607020353147462 1102 40-41 0.466521859307349 1102 42-43 0.4226706589707021 1102 44-45 0.4482959235046806 1102 46-47 0.5141246582783481 1102 48-49 0.41813151971236096 1102 50-51 0.4867088578016805 1102 52-53 0.4089744766980914 1102 54-55 0.3670293820925963 1102 56-57 0.2822686526121174 1102 58-59 0.34346819266365713 1102 60-61 0.56500638624685 1102 62-63 0.5473081208404054 1102 64-65 0.6001367193460752 1102 66-67 0.42879296161655134 1102 68-69 0.5350319549910836 1102 70-71 0.5625081898819104 1102 72-73 0.5484524923102185 1102 74-75 0.5947425828525255 1102 76-77 0.57372077680456 1102 78-79 0.675774343238654 1102 80-81 0.6526407418312594 1102 82-83 0.5730353924809037 1102 84-85 0.5252851250049453 1102 86-87 0.5710861682237223 1102 88-89 0.5954505272602368 1102 90-91 0.6857436724259678 1102 92-93 0.6980637439773894 1102 94-95 0.656559146370082 1102 96-97 0.6117582656736538 1102 98-99 0.742509147984169 1102 100-101 0.7329094945582391 1102 102-103 0.670494649665283 1102 104-105 0.692194346643749 1102 106-107 0.5658407188450596 1102 108-109 0.622197850972448 1102 110-111 0.7580006804361048 1102 112-113 0.7052195312353859 1102 114-115 0.6772762038618012 1102 116-117 0.5623595485356034 1102 118-119 0.5514035864517517 1102 120-121 0.515101232796539 1102 122-123 0.6332592513008564 1102 124-125 0.7089189672582563 1103 1 0.26441803436917155 1103 2 0.534511574823906 1103 3 0.34968443352364886 1103 4 0.504629843227498 1103 5 0.26879502026665847 1103 6 0.1885958244934116 1103 7 0.15779615759385734 1103 8 0.11152327937892181 1103 9 0.30789770793482063 1103 10-11 0.21432929778724485 1103 12-13 0.24506019714984717 1103 14-15 0.3662693467827296 1103 16-17 0.4017469616162259 1103 18-19 0.2584137110940077 1103 20-21 0.17446649614177545 1103 22-23 0.24631192944785596 1103 24-25 0.16941557367286464 1103 26-27 0.2344736303641639 1103 28-29 0.19424503846051522 1103 30-31 0.22425223947725925 1103 32-33 0.14935049929721345 1103 34-35 0.2458857012527389 1103 36-37 0.20304639711507377 1103 38-39 0.006614224232350807 1103 40-41 0.06892391791048169 1103 42-43 0.10594250265474159 1103 44-45 -0.006256341211354766 1103 46-47 0.0779449955774254 1103 48-49 0.09703098624073903 1103 50-51 0.20254458460042457 1103 52-53 0.03501017982219423 1103 54-55 0.12714867646977268 1103 56-57 0.14312646665137407 1103 58-59 0.0715856287310146 1103 60-61 0.0044094884157601655 1103 62-63 -0.06065462777416286 1103 64-65 -0.07070371513786 1103 66-67 -0.016370059008607996 1103 68-69 -0.01722570323146755 1103 70-71 0.012562646482308537 1103 72-73 0.0499671980687495 1103 74-75 0.05819474994785878 1103 76-77 0.10819856549511897 1103 78-79 0.019485138324832008 1103 80-81 -0.2038103965568574 1103 82-83 -0.1759392434721434 1103 84-85 -0.12446866875535534 1103 86-87 -0.2637877495163501 1103 88-89 -0.31656702168260153 1103 90-91 -0.24450346494978348 1103 92-93 -0.21512181113075712 1103 94-95 -0.16160917529186136 1103 96-97 -0.19765230044220772 1103 98-99 -0.2512371342818014 1103 100-101 -0.2514737937289091 1103 102-103 -0.23959983092671777 1103 104-105 -0.09507635491278066 1103 106-107 -0.055981469829852415 1103 108-109 -0.1605281142020729 1103 110-111 -0.08156705451106205 1103 112-113 -0.022413020686592233 1103 114-115 -0.1006973766386281 1103 116-117 -0.1117960769872326 1103 118-119 -0.1304877737448571 1103 120-121 -0.060442008319654406 1103 122-123 0.016600410139293587 1103 124-125 0.04808911206476907 1104 1 0.1662297363599734 1104 2 0.47169850194577023 1104 3 0.01630453290292877 1104 4 0.29932087095077975 1104 5 0.08261364503750457 1104 6 0.09964091502825312 1104 7 0.14116862084924975 1104 8 0.09331178483031266 1104 9 0.19282659064153762 1104 10-11 0.147399040172985 1104 12-13 0.13103885392147419 1104 14-15 0.17266643575774054 1104 16-17 0.2335557188123758 1104 18-19 0.05920890615399088 1104 20-21 0.07775034419439919 1104 22-23 -0.034157147193191406 1104 24-25 0.037395303837207905 1104 26-27 0.0961979332619265 1104 28-29 0.13904245065941012 1104 30-31 0.13294023798174948 1104 32-33 0.05120039316399527 1104 34-35 -0.10960980818668986 1104 36-37 -3.650419548222317E-4 1104 38-39 0.01084573333407235 1104 40-41 0.06078578191523576 1104 42-43 0.11277407447155241 1104 44-45 0.05993017987056248 1104 46-47 -0.10002537757729613 1104 48-49 -0.15752389749514606 1104 50-51 -0.2407877168822239 1104 52-53 -0.05804734116323118 1104 54-55 -0.15686319919355896 1104 56-57 -0.09269228676580354 1104 58-59 -0.17752088238081143 1104 60-61 -0.18990173115135178 1104 62-63 -0.195212396001736 1104 64-65 -0.25693207642496674 1104 66-67 -0.1106233374796517 1104 68-69 -0.18004847483600628 1104 70-71 -0.1812297163485539 1104 72-73 -0.188686297090932 1104 74-75 -0.1941495277030576 1104 76-77 -0.15297054813174427 1104 78-79 -0.2351536670332841 1104 80-81 -0.3048181093057032 1104 82-83 -0.3200990020994894 1104 84-85 -0.3112123691059132 1104 86-87 -0.14751660327764782 1104 88-89 -0.24194210610674816 1104 90-91 -0.2808603720510625 1104 92-93 -0.2931269589936214 1104 94-95 -0.30499931736563823 1104 96-97 -0.3134397666725448 1104 98-99 -0.3452785985985294 1104 100-101 -0.3362878997977994 1104 102-103 -0.4832954075034408 1104 104-105 -0.415753939095719 1104 106-107 -0.5111238551738211 1104 108-109 -0.4603770552820592 1104 110-111 -0.2882632461779977 1104 112-113 -0.2755045525426354 1104 114-115 -0.4000088675690634 1104 116-117 -0.3893130723720475 1104 118-119 -0.4425010785883927 1104 120-121 -0.43843077387781904 1104 122-123 -0.46171564974556745 1104 124-125 -0.3310519425938345 1105 1 0.047678532316446365 1105 2 0.4036541471070585 1105 3 -0.05357385548159499 1105 4 0.19033420718155014 1105 5 -0.053577681985871095 1105 6 -0.0558464972439765 1105 7 0.05899425989374407 1105 8 0.013712918047751543 1105 9 0.11263817714594637 1105 10-11 -0.07437917758504398 1105 12-13 -0.06310612225521339 1105 14-15 0.07793201347327283 1105 16-17 -3.439214232798804E-4 1105 18-19 0.004978656974927276 1105 20-21 -0.10222488679524133 1105 22-23 -0.05358603136033935 1105 24-25 0.03505120171886489 1105 26-27 -0.05723109577677832 1105 28-29 0.014353823880703942 1105 30-31 -0.12671233187852948 1105 32-33 0.016718952818884247 1105 34-35 -0.10451817693306253 1105 36-37 -0.00959959703819635 1105 38-39 -0.15293507199076117 1105 40-41 -0.026686954064416568 1105 42-43 -0.0010683012608510012 1105 44-45 0.10038555943401661 1105 46-47 0.2598269942918705 1105 48-49 0.19118191668295026 1105 50-51 0.1082299735433736 1105 52-53 0.08839767203617299 1105 54-55 0.1604920097434004 1105 56-57 0.2747784169879921 1105 58-59 0.21746535482220253 1105 60-61 0.2189116903926589 1105 62-63 0.17514819330352083 1105 64-65 0.1593145633599775 1105 66-67 0.23097622549119023 1105 68-69 0.38626094261940835 1105 70-71 0.2634479645799104 1105 72-73 0.1855601696529945 1105 74-75 0.1697828897201603 1105 76-77 0.12928550427956154 1105 78-79 0.18478166391771467 1105 80-81 0.23776557413387067 1105 82-83 0.2966604225995013 1105 84-85 0.3130200129042571 1105 86-87 0.2371958267011074 1105 88-89 0.1472351844733808 1105 90-91 0.16206375952449648 1105 92-93 0.05854620677162359 1105 94-95 0.1299900788419457 1105 96-97 0.22081883306346484 1105 98-99 0.22272048388166965 1105 100-101 0.11695883254396833 1105 102-103 -0.04292463694777737 1105 104-105 0.01181330544745407 1105 106-107 0.07418989233116946 1105 108-109 0.11016230953669037 1105 110-111 0.08416578688490972 1105 112-113 0.07168815880885404 1105 114-115 0.24870227868681383 1105 116-117 0.24187867719334122 1105 118-119 0.1643301178896195 1105 120-121 0.11398505977745188 1105 122-123 0.1670731436354096 1105 124-125 0.046378177005674814 1106 1 0.1819616818221732 1106 2 0.5079315758165812 1106 3 0.344166188837697 1106 4 0.47912299017621507 1106 5 0.2069773317179937 1106 6 0.1570182517500669 1106 7 0.10606517364863777 1106 8 0.050806444865550304 1106 9 0.20420178747965423 1106 10-11 0.18026028684037954 1106 12-13 0.1572434154986695 1106 14-15 0.20922850700144124 1106 16-17 0.2617706845508536 1106 18-19 0.19477969771606496 1106 20-21 0.23109783460584765 1106 22-23 0.11381249686113648 1106 24-25 0.2273591908638224 1106 26-27 0.1557653376492638 1106 28-29 0.21964997396815988 1106 30-31 0.1846145069522649 1106 32-33 0.25193580765440515 1106 34-35 0.20110330123191744 1106 36-37 0.2646660331321016 1106 38-39 0.29501870629125193 1106 40-41 0.2350824023411917 1106 42-43 0.2568192585639153 1106 44-45 0.3833634653910707 1106 46-47 0.42848484533699605 1106 48-49 0.42708336173870265 1106 50-51 0.4781386578663742 1106 52-53 0.3042760891989502 1106 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-0.8739246099402926 2216 96-97 -0.9772777099280354 2216 98-99 -0.9430901466731996 2216 100-101 -1.0285117275465048 2216 102-103 -1.1225106223619399 2216 104-105 -1.233826770333188 2216 106-107 -1.2915604471835067 2216 108-109 -1.3087975914845096 2216 110-111 -1.2836868854943049 2216 112-113 -1.3545713077302253 2216 114-115 -1.266948622899843 2216 116-117 -1.3042306779462578 2216 118-119 -1.4102912205473075 2216 120-121 -1.4820462432339596 2216 122-123 -1.6301616845290816 2216 124-125 -1.9126952016607213 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 60.0 3 31.0 4 69.0 5 273.0 6 783.0 7 1369.0 8 2320.0 9 4542.0 10 9169.0 11 12012.0 12 10281.0 13 12743.0 14 18303.0 15 17448.0 16 9235.0 17 7930.0 18 7867.0 19 7470.0 20 7711.0 21 7373.0 22 7651.0 23 8287.0 24 9412.0 25 10565.0 26 12684.0 27 15194.0 28 18813.0 29 23146.0 30 28561.0 31 36067.0 32 46466.0 33 61596.0 34 88166.0 35 142240.0 36 221837.0 37 342789.0 38 141681.0 39 957.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.644955860681556 17.704133708050623 11.322566009135029 22.328344422132794 2 13.352829319953678 23.469901315424814 37.23822129792425 25.93904806669726 3 22.26964443685689 30.367683522123016 28.49663696434761 18.866035076672482 4 13.26427221619081 19.03442536809891 33.87958474644538 33.82171766926489 5 11.087790194523544 39.05732092430647 34.811665943636136 15.043222937533857 6 30.035719327237963 30.797480357326307 20.37989754990396 18.786902765531774 7 22.54288482530129 33.52942611083726 24.673693981454452 19.253995082407005 8 27.52410943455071 31.057770262530294 23.871536566745572 17.546583736173428 9 29.341933824599938 14.528701109525452 22.042330912474384 34.087034153400225 10-11 23.251857493216505 27.46961515423282 26.79619880415387 22.48232854839681 12-13 27.448581280507838 25.46869682088433 27.174899956283415 19.907821942324414 14-15 27.19790848707124 23.065655399225847 27.82103892024721 21.915397193455707 16-17 21.28410337212843 26.89562128813118 32.6827739725015 19.137501367238887 18-19 23.03323994291631 24.095134066119233 34.99794176487934 17.873684226085118 20-21 20.72662008476089 24.15548586376252 35.53150708317675 19.58638696829984 22-23 19.510701714062733 25.760050432303277 31.275011990974804 23.454235862659182 24-25 20.50759699386816 26.86325706654566 30.363993523025997 22.265152416560184 26-27 22.078063647872554 23.109250528970122 31.568596874882214 23.24408894827511 28-29 21.448681342355087 22.522327441551784 34.44318026870899 21.585810947384136 30-31 23.695274779931434 24.200965042520846 32.91827439341187 19.185485784135846 32-33 18.689587843036108 24.629905675925155 34.66005124525072 22.020455235788017 34-35 17.237670076982457 25.793464713079334 35.53287431347486 21.435990896463345 36-37 22.650600361687708 24.27775162386252 32.16666752888365 20.904980485566117 38-39 24.029211418807613 22.814926601931415 31.98209150684243 21.17377047241854 40-41 20.74158571369976 25.284485520476863 31.967581122023088 22.006347643800297 42-43 23.53105939615742 24.4042019036273 31.253764500950037 20.810974199265242 44-45 25.121369358237118 22.331777154846534 31.059950439767615 21.486903047148733 46-47 21.586008731055554 24.46661409606526 34.96967336510726 18.977703807771924 48-49 23.680087443583293 24.270176431766735 33.03482149521728 19.01491462943269 50-51 20.56712691809407 25.201888107391834 32.96682952713803 21.264155447376066 52-53 19.663129359892572 26.749185759230095 28.83243748988435 24.755247390992984 54-55 20.8317043590981 27.467572635006555 27.82918643914978 23.871536566745572 56-57 22.59875648602728 24.08412232346292 28.67125218294865 24.64586900756115 58-59 22.10064141553365 23.229012468396668 31.53537688613045 23.13496922993923 60-61 24.035456333267067 25.039631187915756 30.082159424906195 20.84275305391098 62-63 19.3687684806973 25.429919865553273 31.915503720712646 23.285807933036782 64-65 17.782560207996298 26.283329921417543 33.093612376311896 22.840497494274263 66-67 22.79611795423993 25.160132170473602 29.884834908850117 22.158914966436356 68-69 24.112279866765306 23.64047473174582 29.765885916867994 22.48135948462088 70-71 20.9497295471661 25.85464795310919 29.869536716032286 23.32608578369242 72-73 23.732818171001277 24.976886426068713 29.111056750383007 22.179238652547 74-75 25.000203236861108 22.950171494958617 29.344261810463518 22.705363457716757 76-77 21.418282892407884 24.790425844042684 33.52074235404452 20.270548909504907 78-79 23.17624478882212 24.508370032983496 32.11094367678392 20.204441501410464 80-81 20.088448681953526 25.34271277606032 32.167554380641214 22.40128416134494 82-83 19.34378882285949 26.5918804287337 28.221211868145836 25.843118880260974 84-85 20.400361835517085 27.090143307853587 27.40678633745744 25.102708519171884 86-87 21.96369672330447 23.762712465662208 28.413326130126283 25.86026468090704 88-89 21.5519388427028 23.16656332380214 30.860260985691383 24.421236847803673 90-91 23.143874699671347 24.848477682006 29.43693781912806 22.570709799194592 92-93 19.104006278910443 25.082902163253152 30.91336123467502 24.89973032316139 94-95 17.589443422093133 25.91483197717816 32.083786000931205 24.4119385997975 96-97 22.14888285569747 24.624548388276885 29.42307884178984 23.803489914235808 98-99 23.148070134984124 23.464503846131105 29.11127321425404 24.27615280463073 100-101 20.385950494456807 25.290536330990815 29.42322856904252 24.900284605509864 102-103 22.731759232399455 24.36448092177104 29.039889582590412 23.863870263239097 104-105 23.94313307695293 22.530515243299583 29.103290749053357 24.423060930694128 106-107 20.689121185511077 24.307508197399894 32.74853866009101 22.25483195699802 108-109 22.20825151414017 24.204584388066355 31.25605905543969 22.33110504235379 110-111 19.428487797738832 24.914654576942823 31.512963597409833 24.143894027908516 112-113 18.690066268731513 25.94928451870629 28.08469654642008 27.275952666142118 114-115 19.463378767082016 26.686150771631922 27.285756480972502 26.564713980313563 116-117 20.993473156247966 23.65869959957651 28.048904010741047 27.298923233434476 118-119 20.47348164947219 23.264822774250753 30.13345939207212 26.128236184204933 120-121 21.79330964378833 24.572045228182798 29.02579056003045 24.608854567998417 122-123 18.37192836718359 24.99765347909601 30.027079220762904 26.60333893295749 124-125 17.098756536038284 25.87853592742457 30.948247510300614 26.074460026236533 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3403.0 1 2517.5 2 5247.0 3 7073.5 4 3441.5 5 1630.5 6 2371.0 7 4940.5 8 7490.0 9 7510.0 10 5350.5 11 3237.5 12 2492.5 13 2497.5 14 2846.5 15 3217.0 16 3397.0 17 3615.5 18 3856.5 19 3882.5 20 3971.5 21 4310.0 22 4785.5 23 5280.0 24 5880.5 25 6613.5 26 7548.0 27 8512.5 28 9282.0 29 10412.5 30 12067.0 31 13636.0 32 15225.0 33 16904.5 34 18837.0 35 20254.0 36 21635.5 37 23393.5 38 23794.5 39 23363.0 40 22727.0 41 23210.5 42 24151.0 43 25067.0 44 26853.5 45 28733.5 46 31235.0 47 37652.0 48 46950.5 49 76919.0 50 184478.5 51 186604.5 52 73507.0 53 35326.0 54 27784.5 55 23830.0 56 20788.5 57 17994.0 58 16807.5 59 15686.0 60 13974.5 61 12286.0 62 10782.0 63 8489.0 64 6121.0 65 4898.5 66 3544.5 67 2547.5 68 1888.0 69 1309.0 70 1121.0 71 1042.5 72 1082.5 73 1123.5 74 802.5 75 577.0 76 414.0 77 271.5 78 223.5 79 120.0 80 61.5 81 42.5 82 19.0 83 10.5 84 8.0 85 6.0 86 3.5 87 2.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1506169901581626 2 1.4780862625923713E-4 3 5.912345050369485E-4 4 0.0 5 0.0 6 0.003843024282740165 7 0.0 8 0.0 9 0.0 10-11 3.695215656480928E-5 12-13 0.004988541136249252 14-15 9.977082272498506E-4 16-17 6.651388181665671E-4 18-19 0.0 20-21 3.695215656480928E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 1.1085646969442784E-4 30-31 0.0 32-33 0.0 34-35 3.695215656480928E-5 36-37 0.0 38-39 0.0 40-41 3.695215656480928E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 8.129474444258042E-4 96-97 0.0022540815504533663 98-99 0.003916928595869783 100-101 0.0 102-103 0.003953880752434593 104-105 0.021690915903543048 106-107 0.017219704959201124 108-109 0.01999111670156182 110-111 0.016000283792562416 112-113 0.01481781478248852 114-115 0.040314802812206924 116-117 0.03939099889808669 118-119 0.02161701159041343 120-121 0.013561441459285006 122-123 0.002512746646407031 124-125 0.0019215121413700825 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1353101.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.57403819856169 #Duplication Level Percentage of deduplicated Percentage of total 1 84.95525280262099 41.266196948029766 2 9.879938491892633 9.598170194092653 3 2.4303943795860805 3.5416220829475176 4 0.949188665631393 1.8442370600808433 5 0.44524585699816666 1.081369463279014 6 0.28650962393843915 0.8350157650464772 7 0.16791084457251262 0.5709275444752587 8 0.1315839184740463 0.5113249825819801 9 0.09699408381687587 0.42402548991198796 >10 0.5641372951792677 5.137607266554211 >50 0.052058531050325305 1.749295570542169 >100 0.03058912974862212 2.937440013861101 >500 0.00339879216359329 1.0143665194290832 >1k 0.00339879216359329 4.531283161016555 >5k 0.0017888379808385737 6.3042957384493485 >10k+ 0.0016099541827547163 18.65282219970206 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 54958 4.061633240977577 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 47052 3.477345741374813 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 29024 2.144998784274049 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 17127 1.265759170970977 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 16986 1.2553386628197007 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 14716 1.0875758720154667 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 13206 0.9759803591897426 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11314 0.8361533987485045 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10286 0.7601797648512565 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 9605 0.7098509276099862 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9278 0.685684217216601 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 7885 0.5827355090270423 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7390 0.5461528740278812 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 7370 0.5446747877652888 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 6788 0.5016624775238507 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6323 0.4672969719185781 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 6278 0.4639712778277453 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 6041 0.44645595561602575 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5596 0.41356853627334544 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 4967 0.3670827233148154 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 4905 0.362500655900779 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4710 0.3480893148405034 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 4397 0.3249572648309328 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 3946 0.29162641960947483 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3497 0.2584433830142761 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3251 0.24026292198438995 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3086 0.22806871031800288 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3075 0.22725576287357707 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2669 0.19725061174295194 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 2583 0.19089484081380473 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 1989 0.14699567881481132 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 1883 0.13916182162307175 No Hit TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 1582 0.11691662337105656 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.4780862625923713E-4 0.0 0.0 0.0 0.0 8 1.4780862625923713E-4 0.0 0.0 7.390431312961856E-5 0.0 9 1.4780862625923713E-4 0.0 0.0 7.390431312961856E-5 0.0 10-11 1.4780862625923713E-4 0.0 0.0 2.9561725251847426E-4 0.0 12-13 1.4780862625923713E-4 0.0 0.0 7.759952878609948E-4 0.0 14-15 1.4780862625923713E-4 0.0 0.0 0.0013302776363331341 0.0 16-17 1.4780862625923713E-4 0.0 0.0 0.002143225080758938 0.0 18-19 2.2171293938885568E-4 0.0 0.0 0.003547407030221691 0.0 20-21 2.2171293938885568E-4 0.0 0.0 0.005468919171591773 0.0 22-23 2.2171293938885568E-4 0.0 0.0 0.008868517575554227 0.0 24-25 2.9561725251847426E-4 0.0 0.0 0.012970206954248057 0.0 26-27 2.9561725251847426E-4 0.0 0.0 0.018032652403626927 0.0 28-29 3.695215656480928E-4 0.0 0.0 0.025607844499412828 0.0 30-31 3.695215656480928E-4 0.0 0.0 0.04072127653441983 0.0 32-33 3.695215656480928E-4 0.0 0.0 0.0700982410034432 0.0 34-35 3.695215656480928E-4 0.0 0.0 0.11436692456808471 0.0 36-37 3.695215656480928E-4 0.0 0.0 0.17389684879399248 0.0 38-39 4.4342587877771136E-4 0.0 0.0 0.26428182375151593 0.0 40-41 4.4342587877771136E-4 0.0 0.0 0.3986029128645977 0.0 42-43 5.542823484721392E-4 0.0 0.0 0.49929753950370304 0.0 44-45 6.651388181665671E-4 0.0 0.0 0.609525822536529 0.0 46-47 7.759952878609948E-4 0.0 0.0 0.75559769743722 0.0 48-49 8.129474444258042E-4 0.0 0.0 0.8872212791210707 0.0 50-51 8.129474444258042E-4 0.0 0.0 1.0188079086483566 0.0 52-53 8.129474444258042E-4 0.0 0.0 1.1864967951394612 0.0 54-55 8.129474444258042E-4 0.0 0.0 1.3624999168576477 0.0 56-57 8.129474444258042E-4 0.0 0.0 1.5492930682927586 0.0 58-59 8.129474444258042E-4 0.0 0.0 1.7681976437826887 0.0 60-61 8.129474444258042E-4 0.0 0.0 1.972025739394177 0.0 62-63 8.129474444258042E-4 0.0 0.0 2.1745605095258966 0.0 64-65 8.129474444258042E-4 0.0 0.0 2.4150451444496754 0.0 66-67 8.129474444258042E-4 0.0 0.0 2.678994398792108 0.0 68-69 8.129474444258042E-4 0.0 0.0 3.002547481673578 0.0 70-71 8.129474444258042E-4 0.0 0.0 3.4562091078197414 0.0 72-73 8.129474444258042E-4 0.0 0.0 3.828723798149584 0.0 74-75 8.129474444258042E-4 0.0 0.0 4.173487418899255 0.0 76-77 8.129474444258042E-4 0.0 0.0 4.579591619546509 0.0 78-79 8.129474444258042E-4 0.0 0.0 4.982480982572624 0.0 80-81 9.607560706850413E-4 0.0 0.0 5.416410157113179 0.0 82-83 9.607560706850413E-4 0.0 0.0 5.94242410581324 0.0 84-85 0.0011455168535090877 0.0 0.0 6.448964268003644 0.0 86-87 0.0012563733232035155 0.0 0.0 6.950589793370931 0.0 88-89 0.0012563733232035155 0.0 0.0 7.528299809105159 0.0 90-91 0.0013302776363331341 0.0 0.0 8.089713923794307 0.0 92-93 0.0013302776363331341 0.0 0.0 8.656042675306573 0.0 94-95 0.0013302776363331341 0.0 0.0 9.267416105671344 0.0 96-97 0.0013302776363331341 0.0 0.0 9.878198301531075 0.0 98-99 0.0013302776363331341 0.0 0.0 10.581693458211914 0.0 100-101 0.0013302776363331341 0.0 0.0 11.54961824727053 0.0 102-103 0.0014041819494627528 0.0 0.0 12.358759619570158 0.0 104-105 0.0014780862625923712 0.0 0.0 13.109331823714562 0.0 106-107 0.0014780862625923712 0.0 0.0 13.91030676941337 0.0 108-109 0.0014780862625923712 0.0 0.0 14.631760674184706 0.0 110-111 0.0016258948888516083 0.0 0.0 15.392753386480388 0.0 112-113 0.0016628470454164174 0.0 0.0 16.297785605065698 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 135 0.0 74.92513 2 ATAACGC 180 0.0 62.80489 3 GCGTATC 40 1.7602387E-4 59.58084 1 TAACGCA 260 0.0 45.768745 4 AGTACTC 1190 0.0 41.499565 5 AAGTACT 1305 0.0 39.21031 4 AAAGTAC 1400 0.0 35.699623 3 GTTATCA 675 0.0 33.541805 1 AGCGTCA 120 3.6967613E-5 29.749685 3 TCAAACG 145 4.0679297E-6 28.723835 3 GCAACGC 105 5.906561E-4 28.333035 4 CGCATGT 85 0.0071631763 28.038042 1 GTATAAC 425 0.0 28.038042 1 TATCACG 85 0.007211186 27.999704 2 TTATCAA 895 0.0 26.591896 2 AAAAGTA 1880 0.0 25.951853 2 AAAAAGT 1955 0.0 24.380909 1 CGGGAGT 640 0.0 24.17162 4 GTCGGGA 605 0.0 22.619596 2 GTAAGGC 160 2.6117818E-4 22.312263 3 >>END_MODULE