FastQCFastQC Report
Thu 26 May 2016
SRR1364158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844759
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA377872.0483434421515225No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT267641.4508128161998397No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG180520.9785560065027464No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG94220.5107442218739684No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC80810.43805179972018027No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67600.36644353002207875No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG67390.3653051699436078No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT60390.32735983399457597No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT40850.22143813907399287No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC40780.22105868571450255No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG37270.2020318101172023No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG35640.1931959676033563No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA34530.18717892147429557No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG30220.1638154360542488No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30010.16267707597577785No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC28200.15286549625181392No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG24940.13519381122412197No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC23450.12711687542925662No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA22600.12250922749258847No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21990.11920256250274426No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC1203.6901067E-529.758389
CATGGGG68850.028.5319884
AGTACTC12900.026.7660315
ATGGGCG4400.025.7067185
ATGGGGG34650.025.2557245
CATGGGT39200.022.7786124
ATAACGC2403.5501216E-722.3224323
GTATAGG5750.021.737741
ATGGGGA32950.020.9579245
GGCGCGA1754.775833E-420.4057468
GTCGGGA4700.020.2632332
ATGGGAG24700.020.2454785
GTACACG3251.45028025E-820.1452331
AAGTACT17700.019.5063864
TATAACG1856.939215E-419.3048362
GTACAAA18300.018.8642641
ATGGGTA23500.018.7460425
ATGGGAT26000.018.5463055
TAAGGCG2952.568444E-618.1621065
TGGGGGG21400.018.0775226
Produced by FastQC (version 0.11.5)