##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364158_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1844759 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.35367492447523 31.0 31.0 34.0 26.0 34.0 2 30.4749650225314 31.0 31.0 34.0 26.0 34.0 3 30.592193885488566 31.0 31.0 34.0 26.0 34.0 4 34.00958661808941 37.0 35.0 37.0 30.0 37.0 5 34.03201176955906 37.0 35.0 37.0 30.0 37.0 6 33.94809674326023 37.0 35.0 37.0 29.0 37.0 7 33.87594910771543 37.0 35.0 37.0 29.0 37.0 8 33.800001517813435 37.0 35.0 37.0 28.0 37.0 9 35.21240335458453 39.0 35.0 39.0 28.0 39.0 10-11 35.128439812463306 39.0 35.0 39.0 27.0 39.0 12-13 35.027024126186674 39.0 35.0 39.0 27.0 39.0 14-15 36.21758506124648 40.0 36.0 41.0 27.0 41.0 16-17 36.24961200893992 40.0 36.0 41.0 27.0 41.0 18-19 36.246844709796775 40.0 36.5 41.0 27.0 41.0 20-21 36.24989497273086 40.0 37.0 41.0 27.0 41.0 22-23 36.21218815032208 40.0 36.0 41.0 27.0 41.0 24-25 36.186720867061766 40.0 36.0 41.0 27.0 41.0 26-27 36.06156359719616 40.0 36.0 41.0 27.0 41.0 28-29 35.98604424751417 40.0 36.0 41.0 27.0 41.0 30-31 36.073942449935195 40.0 36.0 41.0 27.0 41.0 32-33 35.870277093105386 40.0 36.0 41.0 27.0 41.0 34-35 35.94178263935831 40.0 36.0 41.0 27.0 41.0 36-37 35.93067007668752 40.0 36.0 41.0 27.0 41.0 38-39 35.849616399757366 40.0 35.0 41.0 27.0 41.0 40-41 35.68280843188731 40.0 35.0 41.0 27.0 41.0 42-43 35.5522406991916 40.0 35.0 41.0 26.5 41.0 44-45 35.39669544910744 40.0 35.0 41.0 26.0 41.0 46-47 35.22784466697276 39.0 35.0 41.0 25.0 41.0 48-49 35.07280815542843 39.0 35.0 41.0 25.0 41.0 50-51 34.958992746477996 39.0 34.5 41.0 24.5 41.0 52-53 34.808113417525 39.0 34.0 41.0 24.0 41.0 54-55 34.602729950091046 39.0 34.0 41.0 24.0 41.0 56-57 34.361007318571154 38.0 34.0 40.5 23.0 41.0 58-59 34.10651472631385 38.0 33.0 40.0 22.5 41.0 60-61 33.86513468696995 38.0 33.0 40.0 22.0 41.0 62-63 33.5030136727887 37.0 33.0 40.0 21.5 41.0 64-65 33.26117097138434 37.0 33.0 40.0 20.0 41.0 66-67 32.942707963479236 36.0 32.5 39.5 19.5 41.0 68-69 32.594014990576 36.0 32.0 39.0 18.0 41.0 70-71 32.27617401514236 35.0 32.0 39.0 15.5 41.0 72-73 32.098669799144496 35.0 32.0 38.5 15.0 40.0 74-75 31.82343384691442 35.0 32.0 37.0 12.5 39.5 76-77 31.4532242422994 35.0 32.0 37.0 8.0 39.0 78-79 31.115259500021413 35.0 32.0 36.0 7.0 39.0 80-81 30.839751967601188 35.0 31.0 36.0 4.0 38.0 82-83 30.580906232196185 35.0 31.0 36.0 2.0 37.0 84-85 30.332720967887948 35.0 31.0 35.0 2.0 37.0 86-87 30.058884114401934 35.0 31.0 35.0 2.0 36.0 88-89 29.845771453073276 35.0 31.0 35.0 2.0 36.0 90-91 29.701703040885015 35.0 31.0 35.0 2.0 36.0 92-93 29.492552685743775 35.0 30.0 35.0 2.0 36.0 94-95 29.413766513674688 35.0 30.0 35.0 2.0 35.0 96-97 29.334637207353374 35.0 30.0 35.0 2.0 35.0 98-99 29.191318486588223 35.0 30.0 35.0 2.0 35.0 100-101 28.947382286791935 35.0 30.0 35.0 2.0 35.0 102-103 27.87146613731116 33.5 28.0 34.5 2.0 35.0 104-105 28.63096100899901 34.0 29.0 35.0 2.0 35.0 106-107 28.703294034613734 35.0 29.5 35.0 2.0 35.0 108-109 28.662864092274383 35.0 30.0 35.0 2.0 35.0 110-111 28.634947166540456 35.0 30.0 35.0 2.0 35.0 112-113 28.54207053604292 35.0 29.5 35.0 2.0 35.0 114-115 28.424032353277582 35.0 29.5 35.0 2.0 35.0 116-117 28.232969997707016 35.0 29.0 35.0 2.0 35.0 118-119 28.076205889224553 35.0 29.0 35.0 2.0 35.0 120-121 27.920978295809913 34.5 29.0 35.0 2.0 35.0 122-123 27.649873235474118 34.5 29.0 35.0 2.0 35.0 124-125 26.761290499192576 33.5 26.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22520164422536126 1101 2 0.30094875740750027 1101 3 0.30915802231944056 1101 4 0.37497879923114397 1101 5 0.208909030380255 1101 6 0.2135362105023475 1101 7 0.28143769684124464 1101 8 0.19676766154643133 1101 9 0.3964534374286188 1101 10-11 0.2695256065940512 1101 12-13 0.34264344035476313 1101 14-15 0.3451756713847871 1101 16-17 0.4095688001446902 1101 18-19 0.43154759767009665 1101 20-21 0.3157824614416711 1101 22-23 0.385068341711964 1101 24-25 0.36143091461664056 1101 26-27 0.5137837019392038 1101 28-29 0.5682811544324551 1101 30-31 0.47956220954046813 1101 32-33 0.5928694548741547 1101 34-35 0.4962348207275582 1101 36-37 0.4572598729097166 1101 38-39 0.4702864460548497 1101 40-41 0.42475581031739296 1101 42-43 0.4870107969214672 1101 44-45 0.4881344482977852 1101 46-47 0.48071891054964055 1101 48-49 0.3471399173580494 1101 50-51 0.3750171421435482 1101 52-53 0.4401571234073103 1101 54-55 0.4886285177654841 1101 56-57 0.5497900593707499 1101 58-59 0.55097656199883 1101 60-61 0.4477418319159412 1101 62-63 0.4460655253491481 1101 64-65 0.4612257085175031 1101 66-67 0.4585151764392634 1101 68-69 0.3372101516143218 1101 70-71 0.30391758786907985 1101 72-73 0.2548200395207658 1101 74-75 0.3134453459709903 1101 76-77 0.25222717420441754 1101 78-79 0.3676479215692723 1101 80-81 0.3963999551985822 1101 82-83 0.23588148823049693 1101 84-85 0.25256183657801756 1101 86-87 0.29555761116927926 1101 88-89 0.3021156524566493 1101 90-91 0.33026254720698134 1101 92-93 0.30232250593297394 1101 94-95 0.37365211605447257 1101 96-97 0.3087365532422339 1101 98-99 0.34607858577728834 1101 100-101 0.33437046414531935 1101 102-103 0.33002449408113677 1101 104-105 0.33990258331252576 1101 106-107 0.31723319483068835 1101 108-109 0.44125759888463634 1101 110-111 0.4930790455821068 1101 112-113 0.4855650224388697 1101 114-115 0.413560227854024 1101 116-117 0.38167391640374504 1101 118-119 0.3851167135015565 1101 120-121 0.4685087226196849 1101 122-123 0.5054905932594238 1101 124-125 0.5213787586977006 1102 1 0.04175702551336258 1102 2 0.13713038902903207 1102 3 0.2005620403073216 1102 4 0.3206913835900167 1102 5 0.08758193596990793 1102 6 0.18252869819696116 1102 7 0.16377022102357586 1102 8 0.24625711546713092 1102 9 0.3508736145741125 1102 10-11 0.2609847791775053 1102 12-13 0.295222419349372 1102 14-15 0.3613698090532651 1102 16-17 0.27825856123905623 1102 18-19 0.31845328927726513 1102 20-21 0.3077123733461775 1102 22-23 0.314505710604557 1102 24-25 0.44768060820222644 1102 26-27 0.45723126006032544 1102 28-29 0.5922904867852665 1102 30-31 0.4327887262647252 1102 32-33 0.5641296804385547 1102 34-35 0.4648189366378901 1102 36-37 0.4312344421398535 1102 38-39 0.38505171633948976 1102 40-41 0.3904791544321782 1102 42-43 0.4504403714989422 1102 44-45 0.505305044661597 1102 46-47 0.452260874682338 1102 48-49 0.5694194454039163 1102 50-51 0.564254349205946 1102 52-53 0.47475700583094493 1102 54-55 0.45758385610123753 1102 56-57 0.3715279118015644 1102 58-59 0.44513098835850684 1102 60-61 0.4242620812993181 1102 62-63 0.48798328914214295 1102 64-65 0.4491421338489161 1102 66-67 0.4572127795734815 1102 68-69 0.4119709314363078 1102 70-71 0.3047533128618767 1102 72-73 0.3300466777261448 1102 74-75 0.3472596685944609 1102 76-77 0.3042951190259586 1102 78-79 0.2930859043753564 1102 80-81 0.2566692853386989 1102 82-83 0.19243870258175377 1102 84-85 0.23105208663388055 1102 86-87 0.28520243075897866 1102 88-89 0.3225000751068059 1102 90-91 0.2574923764609345 1102 92-93 0.3917608076269836 1102 94-95 0.2466346618213997 1102 96-97 0.3175412224556631 1102 98-99 0.2525655146262338 1102 100-101 0.2234945419192691 1102 102-103 0.09595343413671742 1102 104-105 0.18303385667323013 1102 106-107 0.17250173317425066 1102 108-109 0.15365702445250307 1102 110-111 0.1949161220523017 1102 112-113 0.12795959960866554 1102 114-115 0.23704952341540775 1102 116-117 0.2659422746949396 1102 118-119 0.27848341075543104 1102 120-121 0.24433810239351672 1102 122-123 0.2221331806336302 1102 124-125 0.17536122420032285 1103 1 0.33123042930390056 1103 2 0.2836977847764537 1103 3 0.3008980227122038 1103 4 0.3136184904330648 1103 5 0.17594594781080986 1103 6 0.2153090937888038 1103 7 0.3218796193667899 1103 8 0.36898499849026933 1103 9 0.3852199034230779 1103 10-11 0.45968752266085033 1103 12-13 0.43864097904725696 1103 14-15 0.32110946264932494 1103 16-17 0.32734683733923475 1103 18-19 0.3816894828832673 1103 20-21 0.3595205831236541 1103 22-23 0.3231431650236871 1103 24-25 0.4011950502242101 1103 26-27 0.5581908824983657 1103 28-29 0.5943548125507334 1103 30-31 0.37985498075887847 1103 32-33 0.5682143761363179 1103 34-35 0.47820841622213806 1103 36-37 0.41955364567080977 1103 38-39 0.33305409688084353 1103 40-41 0.37409907979783696 1103 42-43 0.35862622635198704 1103 44-45 0.44535941136726365 1103 46-47 0.3558462853949891 1103 48-49 0.28230393906468976 1103 50-51 0.36257859300577167 1103 52-53 0.3713651606236752 1103 54-55 0.3365025852288781 1103 56-57 0.280885123009071 1103 58-59 0.3113086530410314 1103 60-61 0.24120202004885982 1103 62-63 0.27906973881083985 1103 64-65 0.2902322065438767 1103 66-67 0.3149173580130622 1103 68-69 0.34785508836876033 1103 70-71 0.31562052681861985 1103 72-73 0.2223500912562244 1103 74-75 0.1901167657832019 1103 76-77 0.20745668894717184 1103 78-79 0.20081142177547306 1103 80-81 0.24399881662565903 1103 82-83 0.16160967958406403 1103 84-85 0.13915349300742008 1103 86-87 0.07800928509840332 1103 88-89 0.06429072386293555 1103 90-91 0.0450852697883839 1103 92-93 0.05976479721824646 1103 94-95 0.18581976862607874 1103 96-97 0.15276891899951295 1103 98-99 0.21289577478083288 1103 100-101 0.2738780499037752 1103 102-103 0.20262864688294968 1103 104-105 0.2539101068836871 1103 106-107 0.3459972715239843 1103 108-109 0.3063766384510238 1103 110-111 0.313626708321884 1103 112-113 0.22727447468885487 1103 114-115 0.24338688896383687 1103 116-117 0.21351948244992158 1103 118-119 0.16558112164220162 1103 120-121 0.11290270201714847 1103 122-123 0.13076525379318582 1103 124-125 0.11031831265117731 1104 1 0.14863742066443209 1104 2 0.2529796113498364 1104 3 0.28145089382606514 1104 4 0.3033112030236751 1104 5 0.2635238105631288 1104 6 0.2479656131778114 1104 7 0.24627739004436933 1104 8 0.23692543787686304 1104 9 0.29813580768775694 1104 10-11 0.2826898136975373 1104 12-13 0.2932861008457621 1104 14-15 0.3063761047641478 1104 16-17 0.3431829811905516 1104 18-19 0.328546453716406 1104 20-21 0.3460689984459151 1104 22-23 0.2892160343622123 1104 24-25 0.35121197145344496 1104 26-27 0.34918365009288266 1104 28-29 0.47299208427265427 1104 30-31 0.3606948258814171 1104 32-33 0.3889184313663634 1104 34-35 0.292795268757267 1104 36-37 0.3484230592214317 1104 38-39 0.36362042836363173 1104 40-41 0.4447862270080378 1104 42-43 0.39181382928615704 1104 44-45 0.40177165900846035 1104 46-47 0.4617634714989265 1104 48-49 0.38087492078130225 1104 50-51 0.28079390659934234 1104 52-53 0.3245456648313976 1104 54-55 0.33472123287261013 1104 56-57 0.4078782879420544 1104 58-59 0.3761996517924757 1104 60-61 0.3390099613230859 1104 62-63 0.3750004275537506 1104 64-65 0.44832483396033496 1104 66-67 0.3753216551863119 1104 68-69 0.41460497859872447 1104 70-71 0.34151007359386654 1104 72-73 0.231102765497333 1104 74-75 0.2895916660919369 1104 76-77 0.29614011163762655 1104 78-79 0.2829284564469532 1104 80-81 0.3434872565596585 1104 82-83 0.352278843530037 1104 84-85 0.32532175528039886 1104 86-87 0.26038486717019893 1104 88-89 0.2684997772621607 1104 90-91 0.28589622333201703 1104 92-93 0.2643542150170006 1104 94-95 0.3033575197431979 1104 96-97 0.3091311823070484 1104 98-99 0.30067963797804254 1104 100-101 0.19273177334035196 1104 102-103 0.09013325035554232 1104 104-105 0.13618524475009863 1104 106-107 0.1649059724438331 1104 108-109 0.16161241814586802 1104 110-111 0.21040470020920665 1104 112-113 0.1950822653760227 1104 114-115 0.13528764364273727 1104 116-117 0.12886492386284232 1104 118-119 0.013908984526612045 1104 120-121 -0.10341823967501895 1104 122-123 0.031006219494742027 1104 124-125 0.2582066859041703 1105 1 0.1950159642170739 1105 2 0.2509618251499255 1105 3 0.247095637400097 1105 4 0.20499484008622204 1105 5 0.25237523966699626 1105 6 0.2010260608646206 1105 7 0.25173179349530983 1105 8 0.3048301097920003 1105 9 0.3034035980786669 1105 10-11 0.4039713437998742 1105 12-13 0.3848702936799526 1105 14-15 0.4200155311654967 1105 16-17 0.3722411427374226 1105 18-19 0.3349486721766226 1105 20-21 0.3500993727103605 1105 22-23 0.3557411211483341 1105 24-25 0.4462617611433828 1105 26-27 0.5665594702124395 1105 28-29 0.6387873268611841 1105 30-31 0.4669608272040833 1105 32-33 0.23882084287233596 1105 34-35 0.42328756986393046 1105 36-37 0.4739276844573084 1105 38-39 0.4237438165030767 1105 40-41 0.5148323357683608 1105 42-43 0.49123869459273095 1105 44-45 0.44315102581579424 1105 46-47 0.39409862855472966 1105 48-49 0.3756421528274956 1105 50-51 0.3981210851671193 1105 52-53 0.4603086153034255 1105 54-55 0.46673327383339824 1105 56-57 0.46212583380487615 1105 58-59 0.4017270448613104 1105 60-61 0.41570929350562835 1105 62-63 0.4010707658626913 1105 64-65 0.5010330170079911 1105 66-67 0.4009871237260825 1105 68-69 0.43285160335195627 1105 70-71 0.34905724634752744 1105 72-73 0.331635288476015 1105 74-75 0.26761252023134574 1105 76-77 0.31403618713638437 1105 78-79 0.2403586516616265 1105 80-81 0.31092873562669965 1105 82-83 0.27073861782439934 1105 84-85 0.34394238587399784 1105 86-87 0.19817052907541566 1105 88-89 0.1874715029144518 1105 90-91 0.1963222794014463 1105 92-93 0.11532445348275999 1105 94-95 0.14937913333018926 1105 96-97 0.08201457630478615 1105 98-99 0.07819690975748017 1105 100-101 0.08139460109740781 1105 102-103 0.03355102696489354 1105 104-105 0.18803287648611544 1105 106-107 0.19674767050831932 1105 108-109 0.22359154061484432 1105 110-111 0.27955776931178633 1105 112-113 0.2194472903978948 1105 114-115 0.2712906829924897 1105 116-117 0.2211739316986403 1105 118-119 0.2326498349961419 1105 120-121 0.18398439112728582 1105 122-123 0.1236883234972126 1105 124-125 0.23187949473091862 1106 1 -0.034894994702355575 1106 2 0.03267648165126147 1106 3 0.11430243045743538 1106 4 -0.06068215816894451 1106 5 -0.02649613624372904 1106 6 -0.016561323230256164 1106 7 0.04680957055239077 1106 8 -8.505118379531496E-4 1106 9 0.08407088056993928 1106 10-11 0.09663901745657455 1106 12-13 0.06418105970065113 1106 14-15 0.005320161803687995 1106 16-17 -0.03640619777257825 1106 18-19 -0.010225802763315528 1106 20-21 0.005801478616788813 1106 22-23 0.13704218358245868 1106 24-25 0.12672179305791076 1106 26-27 0.18552980053847534 1106 28-29 0.26980599966720575 1106 30-31 0.11626114060860715 1106 32-33 -0.2132363542608786 1106 34-35 -0.005807545712528395 1106 36-37 0.07348319975314155 1106 38-39 0.01854085656870552 1106 40-41 0.06201026608574978 1106 42-43 -0.00891874079353272 1106 44-45 0.01729004561090619 1106 46-47 0.013438813992991072 1106 48-49 0.04386161452521975 1106 50-51 0.02799496538153079 1106 52-53 0.01351171092796477 1106 54-55 0.029187220565816574 1106 56-57 -0.030788020583671027 1106 58-59 0.07139032697781289 1106 60-61 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-2.018364810823517 2216 86-87 -2.0039265312588945 2216 88-89 -2.1355120717039107 2216 90-91 -2.1568213505874745 2216 92-93 -2.2040044308523505 2216 94-95 -2.1513253733038233 2216 96-97 -2.112448062497041 2216 98-99 -2.250223510198502 2216 100-101 -2.3222025189260442 2216 102-103 -2.228730710374702 2216 104-105 -2.318776873738031 2216 106-107 -2.3101606514469424 2216 108-109 -2.2405699623675908 2216 110-111 -2.338527341835075 2216 112-113 -2.418251028552703 2216 114-115 -2.433516744979972 2216 116-117 -2.4369548939630796 2216 118-119 -2.441090623069151 2216 120-121 -2.4182196946245504 2216 122-123 -2.528040657432733 2216 124-125 -2.7404375766150295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56294.0 3 9822.0 4 6269.0 5 4607.0 6 5106.0 7 4708.0 8 4940.0 9 5561.0 10 6603.0 11 7653.0 12 8347.0 13 8918.0 14 9693.0 15 9250.0 16 8627.0 17 8482.0 18 8296.0 19 8491.0 20 8727.0 21 9747.0 22 11607.0 23 14992.0 24 18551.0 25 20216.0 26 23421.0 27 25573.0 28 29340.0 29 35690.0 30 42071.0 31 50510.0 32 64022.0 33 83300.0 34 108922.0 35 151901.0 36 260122.0 37 472423.0 38 230481.0 39 1476.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.596920589824485 17.843922663970858 11.22462874522859 23.334528000976068 2 14.978198616431806 21.60940286102993 38.224747593024865 25.1876509295134 3 20.502042836675987 28.093564805814804 27.89747644462015 23.506915912889063 4 13.059258335543294 17.15497257380405 34.96574516318219 34.820023927470466 5 12.72052997443249 40.075531703438685 33.206176500219186 13.997761821909641 6 31.538725452822586 33.31902889569015 19.270637055754634 15.871608595732631 7 27.527315337645124 33.06737656233494 21.449877321926213 17.955430778093714 8 27.493321205750437 34.19885786490998 20.937182570049046 17.370638359290535 9 28.962500231506954 16.28120305175188 19.440319686588612 35.31597703015256 10-11 24.490210588846363 28.369531851981783 26.099431099339853 21.040826459831997 12-13 25.880988846160026 25.959404282370745 26.028531888172342 22.131074983296887 14-15 24.83240391561746 26.198142834697258 24.06328227730804 24.906170972377247 16-17 22.95199328591136 29.343121626412817 26.791108951991106 20.913776135684717 18-19 23.63733986502253 28.384939831369373 27.552269260101596 20.425451043506506 20-21 22.93800417979945 28.323546430492115 27.327964492127137 21.4104848975813 22-23 22.274043442311317 29.00330953763428 24.993845000778908 23.7288020192755 24-25 22.32845053439567 29.691208102652332 24.729738671816605 23.2506026911354 26-27 23.138974153254722 27.761169722703126 25.149043563940648 23.950812560101507 28-29 22.914763219060667 27.82753028609243 26.496698724014962 22.76100777083194 30-31 24.025686984946837 28.58251758708155 25.84018845256833 21.551606975403278 32-33 21.518887708690798 28.712149685431815 26.922488220715202 22.846474385162185 34-35 20.871204714343527 29.263386547083247 27.15867552612329 22.706733212449937 36-37 23.489879719585257 28.23039917069017 25.948653787101705 22.331067322622864 38-39 24.341451268577618 27.544114825765316 25.768255829932908 22.346178075724158 40-41 22.413247481716443 29.06629427897458 25.85257289546215 22.667885343846827 42-43 23.7488672575523 28.36604793303417 25.440592590979964 22.444492218433563 44-45 24.4135160400076 27.51341907531288 25.54840040920081 22.524664475478712 46-47 22.788576350680685 28.206326761627803 27.634567145682997 21.370529742008515 48-49 23.619417287542767 27.953771318682037 27.12342893482271 21.303382458952484 50-51 21.91344633198958 28.52179901409716 27.37203269647966 22.192721957433605 52-53 21.580797785951145 29.189386044012604 25.179715911271472 24.050100258764783 54-55 21.95981725465539 29.500226594428636 24.781074397823605 23.758881753092368 56-57 22.946091606061497 27.873289203408042 25.129564981695985 24.051054208834476 58-59 22.856980345101707 27.565218550897235 26.301384178815184 23.27641692518587 60-61 23.679507599717372 28.413503882872575 25.95964055258399 21.947347964826065 62-63 21.32521072926247 28.729021316392068 26.794549958397145 23.151217995948315 64-65 20.528479352642872 29.04436914883568 27.171914901262305 23.25523659725914 66-67 22.893504392337512 28.31578339953913 25.990623582908402 22.80008862521496 68-69 23.66799061910019 27.55470558390004 25.883025100657402 22.89427869634236 70-71 21.963381841404793 28.802840251118965 25.84286607950286 23.390911827973383 72-73 23.193452126556213 28.25375160133475 25.61912378882471 22.933672483284326 74-75 23.94433718487738 27.396625189337637 25.641243426948613 23.01779419883637 76-77 22.286330410809303 27.962427568650373 27.814155454614497 21.93708656592583 78-79 22.97906705694895 27.81869076153495 27.31282808943685 21.889414092079253 80-81 21.46203777956711 28.43906810328542 27.37265579021925 22.726238326928218 82-83 21.19882766341952 28.979780269913974 25.37318755071537 24.448204515951133 84-85 21.474647227908193 29.222322634031617 25.007158878097645 24.295871259962546 86-87 22.31598862136081 27.352669760858028 25.71077023587538 24.620571381905787 88-89 22.358518991107534 27.172470181755138 26.671192150046448 23.797818677090884 90-91 23.056175701563422 28.133579177901424 26.138487359092576 22.671757761442578 92-93 21.026058707291902 28.278054276655617 26.901660617507 23.79422639854548 94-95 20.258899588281327 28.408778794787164 27.43995372418252 23.89236789274899 96-97 22.5883113628144 27.66508367900809 26.40465322719125 23.341951730986253 98-99 23.211421351836055 27.041808498598368 26.11447795692372 23.63229219264186 100-101 21.507778327329078 28.035658805906642 26.30326634792691 24.153296518837365 102-103 22.648157643132354 27.428914931842385 26.214685269985218 23.708242155040047 104-105 23.153698641315433 26.683096005548983 26.334652576604512 23.82855277653107 106-107 21.723718672997748 27.314417174993196 28.200253399219672 22.76161075278939 108-109 22.443884507066013 27.344751376104604 27.45646677337582 22.75489734345356 110-111 20.9071051426354 27.93020225106802 27.788479828365652 23.37421277793092 112-113 20.635751670660433 28.343810733044272 25.957320129505778 25.06311746678952 114-115 20.78895287469452 29.02518049920176 25.510871313246152 24.674995312857572 116-117 21.543518924559653 27.428233791190554 25.905776008687788 25.122471275562003 118-119 21.442357897671187 27.20833762094467 26.812979021466372 24.536325459917776 120-121 22.01058385436773 27.9960861845254 26.44923610358652 23.544093857520345 122-123 20.419319752400735 28.175614025109873 27.040970020482696 24.36409620200669 124-125 19.91958554117989 28.577908717942858 27.295310072678326 24.207195668198928 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8394.0 1 5046.5 2 2543.0 3 3347.5 4 2992.5 5 2352.0 6 1838.0 7 1590.0 8 1521.0 9 1437.5 10 1272.5 11 1195.0 12 1208.5 13 1204.5 14 1239.0 15 1350.0 16 1473.5 17 1606.5 18 1836.0 19 2095.5 20 2414.5 21 2976.5 22 3767.0 23 4653.5 24 5621.0 25 6880.0 26 8568.5 27 10671.0 28 12670.0 29 14792.0 30 17878.0 31 21335.5 32 24617.5 33 27514.0 34 30854.0 35 33999.0 36 37668.0 37 41119.0 38 42877.0 39 44089.0 40 44512.0 41 46655.5 42 49152.0 43 51633.5 44 55317.0 45 58446.0 46 61186.5 47 65054.5 48 70766.5 49 86647.5 50 140026.0 51 142956.0 52 84759.0 53 59804.5 54 52715.0 55 48063.0 56 43068.0 57 38174.5 58 35334.0 59 32075.0 60 27815.5 61 23860.0 62 20373.5 63 16555.5 64 12744.5 65 9859.5 66 7207.0 67 5569.0 68 4297.0 69 2925.0 70 2314.5 71 1913.0 72 1596.0 73 1461.5 74 1135.0 75 833.0 76 582.5 77 349.0 78 223.0 79 143.5 80 78.0 81 46.5 82 40.5 83 31.0 84 23.0 85 22.0 86 17.0 87 12.5 88 13.0 89 9.0 90 9.5 91 10.5 92 7.5 93 7.5 94 5.0 95 3.0 96 2.5 97 2.0 98 1.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.478273855826154 2 0.46819123798826845 3 0.4521999892668907 4 0.45404304844155796 5 0.4577291667908925 6 0.4607105860440307 7 0.4469418498568105 8 0.4729073011705052 9 0.48575450777039164 10-11 0.489250899439981 12-13 0.4891424841944124 14-15 0.4647490539414633 16-17 0.46726970840093474 18-19 0.4541785674985188 20-21 0.4632854481262864 22-23 0.4799000845096839 24-25 0.4672426045895426 26-27 0.4771625995590752 28-29 0.4730428202274661 30-31 0.48599844207292114 32-33 0.4829357113856065 34-35 0.47865330918564436 36-37 0.43642557103665036 38-39 0.3993204532407756 40-41 0.3928697461294402 42-43 0.40135323909518805 44-45 0.4340946432569241 46-47 0.44168371044673044 48-49 0.4394611979125729 50-51 0.4262887455759804 52-53 0.42078667186337076 54-55 0.42154557858235137 56-57 0.4042262431027576 58-59 0.4083189186229746 60-61 0.4183202250266837 62-63 0.4198651422760371 64-65 0.43357967084047294 66-67 0.4233615339456265 68-69 0.4247167245152348 70-71 0.434745134730336 72-73 0.4342030585024927 74-75 0.4387022911935923 76-77 0.4300561753594914 78-79 0.4227110424722146 80-81 0.4307337706442956 82-83 0.4290262305265891 84-85 0.4271018599177454 86-87 0.43279366031010014 88-89 0.42035301088109617 90-91 0.42422885591017584 92-93 0.43379650133161024 94-95 0.4307608744556877 96-97 0.4017055886432862 98-99 0.37419522008023814 100-101 0.3738157667207478 102-103 0.3554122787854674 104-105 0.35690298841203644 106-107 0.37660745929414086 108-109 0.38235346730928 110-111 0.399212037995207 112-113 0.39636613779902957 114-115 0.39492963579524476 116-117 0.3673650596094124 118-119 0.4375639311151213 120-121 0.4438520153581037 122-123 0.3986699617673637 124-125 0.39455018243575446 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1844759.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.30290897552544 #Duplication Level Percentage of deduplicated Percentage of total 1 86.50145699710899 54.75793858538318 2 9.12847137374866 11.55717584916202 3 2.105750623074761 3.9990042005297273 4 0.791960960050331 2.0053373026494334 5 0.3774915085353142 1.1948155301922392 6 0.22393080149553896 0.8505282686333134 7 0.1551939293455588 0.6876959028041225 8 0.10325641366493794 0.5229145084296614 9 0.07640368529877899 0.4352917982276967 >10 0.472522707063588 5.6235637715687385 >50 0.041776653125476126 1.8093689587356858 >100 0.017595051701988662 1.9138217391004901 >500 8.3785915721038E-4 0.3188523192020988 >1k 0.0022342910858943466 3.747376633106563 >5k 6.982159643419833E-4 3.274357847398099 >10k+ 4.1892957860519E-4 7.3019567848768965 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 37787 2.0483434421515225 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 26764 1.4508128161998397 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 18052 0.9785560065027464 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 9422 0.5107442218739684 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 8081 0.43805179972018027 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6760 0.36644353002207875 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 6739 0.3653051699436078 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 6039 0.32735983399457597 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 4085 0.22143813907399287 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 4078 0.22105868571450255 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 3727 0.2020318101172023 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 3564 0.1931959676033563 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 3453 0.18717892147429557 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 3022 0.1638154360542488 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 3001 0.16267707597577785 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 2820 0.15286549625181392 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 2494 0.13519381122412197 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 2345 0.12711687542925662 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 2260 0.12250922749258847 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2199 0.11920256250274426 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 5.420762278433118E-5 0.0 0.0 1.0841524556866235E-4 0.0 12-13 5.420762278433118E-5 0.0 0.0 2.710381139216559E-4 0.0 14-15 1.0841524556866235E-4 0.0 0.0 5.691800392354774E-4 0.0 16-17 1.6262286835299353E-4 0.0 0.0 0.0011654638898631203 0.0 18-19 1.6262286835299353E-4 0.0 0.0 0.0019514744202359224 0.0 20-21 1.6262286835299353E-4 0.0 0.0 0.00300852306453038 0.0 22-23 1.6262286835299353E-4 0.0 0.0 0.005014205107550634 0.0 24-25 1.6262286835299353E-4 0.0 0.0 0.006803056659433563 0.0 26-27 1.6262286835299353E-4 0.0 0.0 0.008619012022708657 0.0 28-29 1.8972667974515912E-4 0.0 0.0 0.0117359503328077 0.0 30-31 2.710381139216559E-4 0.0 0.0 0.018132449821358778 0.0 32-33 2.710381139216559E-4 0.0 0.0 0.03238905461363788 0.0 34-35 3.7945335949031824E-4 0.0 0.0 0.053367404631174044 0.0 36-37 4.878686050589806E-4 0.0 0.0 0.08586487449038058 0.0 38-39 4.878686050589806E-4 0.0 0.0 0.13505829216716114 0.0 40-41 5.420762278433118E-4 0.0 0.0 0.20059530811341753 0.0 42-43 5.691800392354774E-4 0.0 0.0 0.2577030387167104 0.0 44-45 6.775952848041397E-4 0.0 0.0 0.32557098244269306 0.0 46-47 8.131143417649677E-4 0.0 0.0 0.41276394369128977 0.0 48-49 8.131143417649677E-4 0.0 0.0 0.49686707044117956 0.0 50-51 8.131143417649677E-4 0.0 0.0 0.5865264785264633 0.0 52-53 8.131143417649677E-4 0.0 0.0 0.6868918921116525 0.0 54-55 8.131143417649677E-4 0.0 0.0 0.8013241838093756 0.0 56-57 8.131143417649677E-4 0.0 0.0 0.9348646625385755 0.0 58-59 8.131143417649677E-4 0.0 0.0 1.0836374832701723 0.0 60-61 8.673219645492988E-4 0.0 0.0 1.231895331585318 0.0 62-63 8.673219645492988E-4 0.0 0.0 1.380830775185268 0.0 64-65 9.2152958733363E-4 0.0 0.0 1.5493893782331458 0.0 66-67 0.0010299448329022924 0.0 0.0 1.751366980727564 0.0 68-69 0.0011383600784709547 0.0 0.0 1.9899889362241896 0.0 70-71 0.0012738791354317827 0.0 0.0 2.284959715605128 0.0 72-73 0.0013551905696082794 0.0 0.0 2.5437469067775247 0.0 74-75 0.0013551905696082794 0.0 0.0 2.797438581408195 0.0 76-77 0.0013551905696082794 0.0 0.0 3.097369358273899 0.0 78-79 0.0013551905696082794 0.0 0.0 3.421422527278631 0.0 80-81 0.0014636058151769418 0.0 0.0 3.7637978727844668 0.0 82-83 0.0014636058151769418 0.0 0.0 4.1401071901532935 0.0 84-85 0.0014636058151769418 0.0 0.0 4.517066998995533 0.0 86-87 0.0014636058151769418 0.0 0.0 4.9150322616667 0.0 88-89 0.001517813437961273 0.0 0.0 5.36422372786906 0.0 90-91 0.001707540117706432 0.0 0.0 5.819459344011873 0.0 92-93 0.0017346439290985977 0.0 0.0 6.28212140447614 0.0 94-95 0.0017346439290985977 0.0 0.0 6.776305197589496 0.0 96-97 0.0017346439290985977 0.0 0.0 7.284854010740698 0.0 98-99 0.00184305917466726 0.0 0.0 7.853627492805293 0.0 100-101 0.0018701629860594256 0.0 0.0 8.556944294620598 0.0 102-103 0.0018972667974515912 0.0 0.0 9.202746808661729 0.0 104-105 0.0022225125341575783 0.0 0.0 9.82068660459171 0.0 106-107 0.0022496163455497436 0.0 0.0 10.451500710932972 5.420762278433118E-5 108-109 0.0022767201569419094 0.0 0.0 11.088928147253924 5.420762278433118E-5 110-111 0.002439343025294903 0.0 0.0 11.769098294140319 5.420762278433118E-5 112-113 0.002439343025294903 0.0 0.0 12.522530043219739 5.420762278433118E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCGAC 120 3.6901067E-5 29.75838 9 CATGGGG 6885 0.0 28.531988 4 AGTACTC 1290 0.0 26.766031 5 ATGGGCG 440 0.0 25.706718 5 ATGGGGG 3465 0.0 25.255724 5 CATGGGT 3920 0.0 22.778612 4 ATAACGC 240 3.5501216E-7 22.322432 3 GTATAGG 575 0.0 21.73774 1 ATGGGGA 3295 0.0 20.957924 5 GGCGCGA 175 4.775833E-4 20.405746 8 GTCGGGA 470 0.0 20.263233 2 ATGGGAG 2470 0.0 20.245478 5 GTACACG 325 1.45028025E-8 20.145233 1 AAGTACT 1770 0.0 19.506386 4 TATAACG 185 6.939215E-4 19.304836 2 GTACAAA 1830 0.0 18.864264 1 ATGGGTA 2350 0.0 18.746042 5 ATGGGAT 2600 0.0 18.546305 5 TAAGGCG 295 2.568444E-6 18.162106 5 TGGGGGG 2140 0.0 18.077522 6 >>END_MODULE