##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364158_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1844759 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08693926957397 33.0 31.0 34.0 30.0 34.0 2 31.901204981246874 33.0 31.0 34.0 30.0 34.0 3 32.12177959288991 34.0 31.0 34.0 30.0 34.0 4 35.62102420966641 37.0 35.0 37.0 33.0 37.0 5 35.746476911076186 37.0 35.0 37.0 33.0 37.0 6 35.861565115009604 37.0 35.0 37.0 35.0 37.0 7 35.84236802747676 37.0 35.0 37.0 35.0 37.0 8 35.8714921569701 37.0 35.0 37.0 35.0 37.0 9 37.52871404882698 39.0 37.0 39.0 35.0 39.0 10-11 37.49333327551187 39.0 37.0 39.0 34.0 39.0 12-13 37.46898510862394 39.0 37.0 39.0 34.0 39.0 14-15 38.7674333070065 40.0 38.0 41.0 34.0 41.0 16-17 38.76689231493111 40.0 38.0 41.0 34.0 41.0 18-19 38.872783111506706 40.0 38.0 41.0 34.5 41.0 20-21 38.93033453150249 40.0 38.0 41.0 35.0 41.0 22-23 38.886619336184296 40.0 38.0 41.0 35.0 41.0 24-25 38.85431809791957 40.0 38.0 41.0 35.0 41.0 26-27 38.71299123625362 40.0 38.0 41.0 34.5 41.0 28-29 38.585452083442874 40.0 38.0 41.0 34.0 41.0 30-31 38.46931387785613 40.0 38.0 41.0 34.0 41.0 32-33 38.254552491680485 40.0 37.5 41.0 34.0 41.0 34-35 38.08785673358959 40.0 37.0 41.0 33.5 41.0 36-37 37.97838986013892 40.0 37.0 41.0 33.0 41.0 38-39 37.893695870300675 40.0 37.0 41.0 33.0 41.0 40-41 37.73273338143356 40.0 37.0 41.0 33.0 41.0 42-43 37.58829581533414 40.0 37.0 41.0 33.0 41.0 44-45 37.37185643219521 40.0 37.0 41.0 33.0 41.0 46-47 37.06729415603881 40.0 36.5 41.0 31.5 41.0 48-49 36.75712789583897 40.0 36.0 41.0 31.0 41.0 50-51 36.56805062341476 40.0 36.0 41.0 31.0 41.0 52-53 36.433322997746586 40.0 35.5 41.0 30.0 41.0 54-55 36.26235811832332 39.5 35.0 41.0 30.0 41.0 56-57 36.04607837663348 39.0 35.0 41.0 29.5 41.0 58-59 35.8941306154354 39.0 35.0 41.0 29.0 41.0 60-61 35.748121028275236 39.0 35.0 41.0 29.0 41.0 62-63 35.46949981000228 38.0 35.0 40.5 29.0 41.0 64-65 35.18621998862724 38.0 35.0 40.0 28.5 41.0 66-67 34.82848816566283 37.0 35.0 40.0 28.0 41.0 68-69 34.515491183401195 37.0 34.5 39.5 28.0 41.0 70-71 34.18403786077205 36.0 34.0 39.0 28.0 41.0 72-73 33.922600458921735 36.0 34.0 39.0 28.0 40.5 74-75 33.59852262544864 35.0 34.0 37.5 28.0 40.0 76-77 33.21457436987704 35.0 34.0 37.0 27.0 39.0 78-79 32.87827569888533 35.0 34.0 36.5 26.5 39.0 80-81 32.62049026458199 35.0 34.0 36.0 26.5 38.0 82-83 32.37752980199582 35.0 34.0 36.0 26.0 37.0 84-85 32.15467765708149 35.0 33.5 35.0 26.0 37.0 86-87 31.934886616625803 35.0 33.0 35.0 26.0 36.5 88-89 31.76932705030847 35.0 33.0 35.0 25.5 36.0 90-91 31.637074002620395 35.0 33.0 35.0 25.0 36.0 92-93 31.490945971804447 35.0 33.0 35.0 25.0 36.0 94-95 31.408291814811584 35.0 33.0 35.0 25.0 35.5 96-97 31.33391136728429 35.0 33.0 35.0 24.0 35.0 98-99 31.258124231945743 35.0 33.0 35.0 24.0 35.0 100-101 31.13797818576844 35.0 33.0 35.0 23.5 35.0 102-103 29.96954805478656 33.5 30.5 35.0 19.0 35.0 104-105 30.819359873024066 35.0 32.5 35.0 20.0 35.0 106-107 30.921795475723385 35.0 33.0 35.0 20.0 35.0 108-109 30.889538145633114 35.0 33.0 35.0 19.0 35.0 110-111 30.856641978708332 35.0 33.0 35.0 19.0 35.0 112-113 30.787659797296016 35.0 33.0 35.0 18.5 35.0 114-115 30.71897684196147 35.0 33.0 35.0 17.5 35.0 116-117 30.58745505510476 35.0 33.0 35.0 12.5 35.0 118-119 30.478302856904342 35.0 33.0 35.0 4.5 35.0 120-121 30.360111808642756 35.0 33.0 35.0 2.0 35.0 122-123 30.149862664987676 35.0 33.0 35.0 2.0 35.0 124-125 29.2540719411045 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34934266131593006 1101 2 0.696821049455675 1101 3 0.4684417847052842 1101 4 0.5431420084374565 1101 5 0.20334171574005921 1101 6 0.2443499761732255 1101 7 0.2501310646192181 1101 8 0.1604639755568087 1101 9 0.2821377049438709 1101 10-11 0.280133832881198 1101 12-13 0.2830018031104018 1101 14-15 0.3534994073297639 1101 16-17 0.40407400496717827 1101 18-19 0.24100142191947782 1101 20-21 0.22289696705330897 1101 22-23 0.2582387610093164 1101 24-25 0.22491231274033652 1101 26-27 0.30368330414446376 1101 28-29 0.43291318106376053 1101 30-31 0.37191168884857007 1101 32-33 0.41517366726939287 1101 34-35 0.40952270623773046 1101 36-37 0.3711079023306354 1101 38-39 0.38757451521230024 1101 40-41 0.44100156709720295 1101 42-43 0.4212854259166505 1101 44-45 0.397390426360154 1101 46-47 0.4505965800013527 1101 48-49 0.387498924130405 1101 50-51 0.41042927496919646 1101 52-53 0.3923843395718549 1101 54-55 0.27027678523904086 1101 56-57 0.30581718326183704 1101 58-59 0.3743885423565416 1101 60-61 0.32857308000896523 1101 62-63 0.3367145930756692 1101 64-65 0.30735300714051306 1101 66-67 0.28397536857793426 1101 68-69 0.28574327730289895 1101 70-71 0.3178776143530584 1101 72-73 0.2627142236270146 1101 74-75 0.22135971085391049 1101 76-77 0.24400837643797502 1101 78-79 0.2593996381873751 1101 80-81 0.2616982769518188 1101 82-83 0.2163180541157601 1101 84-85 0.1724741863938135 1101 86-87 0.21929623923332286 1101 88-89 0.28822193567636845 1101 90-91 0.29604258327905697 1101 92-93 0.27828466957002007 1101 94-95 0.19925168677201555 1101 96-97 0.1756577299925297 1101 98-99 0.23001616099365307 1101 100-101 0.23442276139643425 1101 102-103 0.20538085614434465 1101 104-105 0.28311866682177467 1101 106-107 0.29975408692627425 1101 108-109 0.30496378102228405 1101 110-111 0.257034037764015 1101 112-113 0.26892546420310737 1101 114-115 0.24420753506946724 1101 116-117 0.2891716401510678 1101 118-119 0.26090954716560333 1101 120-121 0.3317817817697417 1101 122-123 0.3821436599445107 1101 124-125 0.2719702712882466 1102 1 0.19304039460848088 1102 2 0.5002331502763262 1102 3 0.2856734470224964 1102 4 0.41391647223933603 1102 5 0.24107624479465528 1102 6 0.22243402375043786 1102 7 0.1726770918613596 1102 8 0.07628895164021543 1102 9 0.2078124132264989 1102 10-11 0.1335844931093746 1102 12-13 0.1889198315586711 1102 14-15 0.2961844545230079 1102 16-17 0.31135083222755355 1102 18-19 0.13482448684462867 1102 20-21 0.16894308215233167 1102 22-23 0.15259791558752767 1102 24-25 0.19271222466652915 1102 26-27 0.1485906964872541 1102 28-29 0.30181200898793037 1102 30-31 0.263036679900182 1102 32-33 0.24484873782605376 1102 34-35 0.25105628600417873 1102 36-37 0.3297750375673161 1102 38-39 0.26552287272253494 1102 40-41 0.24984696502378512 1102 42-43 0.28642810114774164 1102 44-45 0.4786897645050132 1102 46-47 0.4685814794648877 1102 48-49 0.399328147714229 1102 50-51 0.3509526091051782 1102 52-53 0.3093153903618031 1102 54-55 0.3703381765882767 1102 56-57 0.39842999257770373 1102 58-59 0.5081072528345061 1102 60-61 0.3726880792388556 1102 62-63 0.38182665940435356 1102 64-65 0.43540196619921545 1102 66-67 0.35280391099978914 1102 68-69 0.4471217191774386 1102 70-71 0.31766420891200653 1102 72-73 0.3418290710055203 1102 74-75 0.36620614570858123 1102 76-77 0.2604449340102519 1102 78-79 0.25536066227317633 1102 80-81 0.2653061033726658 1102 82-83 0.3649331151708637 1102 84-85 0.3067851173079319 1102 86-87 0.3132374770825024 1102 88-89 0.29898467438134446 1102 90-91 0.2251049334613846 1102 92-93 0.1940356180989049 1102 94-95 0.18705205425238702 1102 96-97 0.2130039533398964 1102 98-99 0.2385716312231878 1102 100-101 0.2718777211883534 1102 102-103 0.19739808260032987 1102 104-105 0.22625438725901859 1102 106-107 0.3002782453983919 1102 108-109 0.29536894223423005 1102 110-111 0.3090754085916281 1102 112-113 0.36187749868638974 1102 114-115 0.35663613779802006 1102 116-117 0.3757315444413045 1102 118-119 0.38335302048422903 1102 120-121 0.3138606056590021 1102 122-123 0.30879808088091565 1102 124-125 0.3524900152234096 1103 1 0.26647700550185505 1103 2 0.547967586714595 1103 3 0.3880297993596642 1103 4 0.4837225977111288 1103 5 0.15722929421060172 1103 6 0.12450415701386675 1103 7 0.19773990874295322 1103 8 0.13530485246306512 1103 9 0.1932158798232635 1103 10-11 0.14976663329490947 1103 12-13 0.16339055004740288 1103 14-15 0.29960075515746354 1103 16-17 0.29614850752340516 1103 18-19 0.2206113548663069 1103 20-21 0.08135269297537207 1103 22-23 0.16902727403483198 1103 24-25 0.11826356401408589 1103 26-27 0.02549118687451113 1103 28-29 0.08405720901095037 1103 30-31 0.044306859623603145 1103 32-33 0.1700548240839126 1103 34-35 0.23136593753299195 1103 36-37 0.2202341708968092 1103 38-39 0.20600480980549207 1103 40-41 0.18254818683610807 1103 42-43 0.1030877881608987 1103 44-45 0.17317114960925295 1103 46-47 0.2649969745087972 1103 48-49 0.19290889111659482 1103 50-51 0.2760199014897893 1103 52-53 0.08095311544230555 1103 54-55 0.14133334730740188 1103 56-57 0.04154192703929027 1103 58-59 -0.0032306881357442307 1103 60-61 0.03333919033576649 1103 62-63 0.10642578220576837 1103 64-65 0.14621973650361042 1103 66-67 0.13773840881638222 1103 68-69 0.12811186028365285 1103 70-71 0.0793838996334415 1103 72-73 0.11554390900296596 1103 74-75 0.13796055354625025 1103 76-77 0.043972045968160955 1103 78-79 -0.006504215897237486 1103 80-81 -0.02918359338003995 1103 82-83 -0.10261655758461785 1103 84-85 -0.15125757472844015 1103 86-87 -0.054912072916323496 1103 88-89 -0.09654856049092686 1103 90-91 -0.11485693951531672 1103 92-93 -0.10358293477916547 1103 94-95 0.05379288639758073 1103 96-97 0.0018731642234683932 1103 98-99 -0.008542468529380898 1103 100-101 -0.09543826637400699 1103 102-103 -0.20405453780668736 1103 104-105 -0.013027848617035431 1103 106-107 0.07828823429487741 1103 108-109 0.09842096889117968 1103 110-111 0.08806707776244593 1103 112-113 0.08529594116871664 1103 114-115 0.0792058331015113 1103 116-117 0.041468478636488726 1103 118-119 0.08200434896270892 1103 120-121 0.1389979076979131 1103 122-123 0.2408404979935348 1103 124-125 0.31724889217052166 1104 1 0.18450415132771525 1104 2 0.48427679318423245 1104 3 -7.002392698751692E-4 1104 4 0.36122029531436084 1104 5 0.13465272862043065 1104 6 0.15635952686887578 1104 7 0.10206072679522293 1104 8 0.07091356495433843 1104 9 0.23508568490021275 1104 10-11 0.17790917371432613 1104 12-13 0.1795517769989985 1104 14-15 0.2209709335317882 1104 16-17 0.24707469714790875 1104 18-19 0.19399753720978907 1104 20-21 0.07820033797882076 1104 22-23 0.08447370123813869 1104 24-25 0.11364681598637816 1104 26-27 0.1352737708743419 1104 28-29 0.24353748289973254 1104 30-31 0.1644623156566709 1104 32-33 0.2092174306242569 1104 34-35 0.18855093451860938 1104 36-37 0.14301055493712056 1104 38-39 0.15124501728802642 1104 40-41 0.2268585589023644 1104 42-43 0.25575036537638596 1104 44-45 0.19139654196778366 1104 46-47 0.22972222182821156 1104 48-49 0.31076716809426586 1104 50-51 0.17774058311547236 1104 52-53 0.23276071485749839 1104 54-55 0.3374585179066898 1104 56-57 0.28863024713769647 1104 58-59 0.2994829037458686 1104 60-61 0.325792256050363 1104 62-63 0.4082519011864463 1104 64-65 0.27985240343199536 1104 66-67 0.24744609557206587 1104 68-69 0.29953969764753197 1104 70-71 0.30747117196298746 1104 72-73 0.25868793151599334 1104 74-75 0.29171904784481484 1104 76-77 0.3076704481074515 1104 78-79 0.36065977551560024 1104 80-81 0.3455017589381839 1104 82-83 0.24873975621343902 1104 84-85 0.25699647143996884 1104 86-87 0.23905498709076767 1104 88-89 0.23365912283031776 1104 90-91 0.19499044975432867 1104 92-93 0.23683739066851572 1104 94-95 0.28348549595494177 1104 96-97 0.24775256090304865 1104 98-99 0.2551532256848539 1104 100-101 0.2619347266572447 1104 102-103 0.2144875100609731 1104 104-105 0.3029987614359442 1104 106-107 0.3718107322645956 1104 108-109 0.3684680682939572 1104 110-111 0.3871672408719995 1104 112-113 0.3372875209748365 1104 114-115 0.2955596001290779 1104 116-117 0.3226088902305335 1104 118-119 0.30199802959029043 1104 120-121 0.21688912625361922 1104 122-123 0.160739678641459 1104 124-125 0.20012875593414847 1105 1 0.14860764776251045 1105 2 0.49172793038681206 1105 3 0.05339740536401649 1105 4 0.337111473389875 1105 5 0.125169380392272 1105 6 0.09868959365061869 1105 7 0.09638800165846106 1105 8 0.05914563622805247 1105 9 0.14192418286260988 1105 10-11 0.07956759982030093 1105 12-13 0.14607084694342376 1105 14-15 0.24832105277051397 1105 16-17 0.21910762421664032 1105 18-19 0.1417851913003858 1105 20-21 0.12209872118977216 1105 22-23 0.09080314580308624 1105 24-25 0.08468014162213677 1105 26-27 0.15643252101907734 1105 28-29 0.24511362252268754 1105 30-31 0.2300430311981927 1105 32-33 0.10862421941504863 1105 34-35 0.16018769869493354 1105 36-37 0.15549082314011997 1105 38-39 -0.023908233489386532 1105 40-41 0.0623232793524906 1105 42-43 0.13320939204893278 1105 44-45 0.19260380890906248 1105 46-47 0.22718098339585424 1105 48-49 0.20485535472084138 1105 50-51 0.2464506411305578 1105 52-53 0.12103186311390601 1105 54-55 0.1626746596244928 1105 56-57 0.19297058621466334 1105 58-59 0.09912909266144254 1105 60-61 0.16802553355139338 1105 62-63 0.19275033375370043 1105 64-65 0.12141271733180048 1105 66-67 0.19589469257583403 1105 68-69 0.20911787286029693 1105 70-71 0.10720715866719388 1105 72-73 0.08771313688457383 1105 74-75 0.08807195809237811 1105 76-77 0.1417290079614446 1105 78-79 0.12061612157469881 1105 80-81 0.09850806804504231 1105 82-83 0.09736751994830684 1105 84-85 0.1071723698434468 1105 86-87 0.131516027391001 1105 88-89 0.18553731960235353 1105 90-91 0.1978160261918056 1105 92-93 0.16966630560843754 1105 94-95 0.10656630863662997 1105 96-97 0.11067838078220049 1105 98-99 0.11804714200813038 1105 100-101 0.1670256115059381 1105 102-103 0.047647637191772674 1105 104-105 0.21065421134950313 1105 106-107 0.08213196793414923 1105 108-109 0.15592651407487068 1105 110-111 0.18310871870448509 1105 112-113 0.09532419563926453 1105 114-115 0.17700767588982202 1105 116-117 0.15980595427168964 1105 118-119 0.14144971987446908 1105 120-121 0.12212991233124626 1105 122-123 0.10904662645621244 1105 124-125 0.026589396237866936 1106 1 0.06868042341919534 1106 2 0.34181415328698606 1106 3 0.25764478096458987 1106 4 0.3543376631826476 1106 5 0.10258591416458529 1106 6 0.07693841015154135 1106 7 0.017252179031849835 1106 8 -0.043032911653554606 1106 9 0.07991514331290261 1106 10-11 0.11746473275665181 1106 12-13 0.014724256233428434 1106 14-15 0.17886216328766835 1106 16-17 0.14011016331267712 1106 18-19 0.029073569934610077 1106 20-21 0.006256347436924159 1106 22-23 0.045697659123952405 1106 24-25 -0.06345450378043438 1106 26-27 -0.04535385494258293 1106 28-29 0.0059811996057632655 1106 30-31 -0.06523971008115836 1106 32-33 -0.05139984198053327 1106 34-35 -0.03707119839253181 1106 36-37 -0.07225120549747288 1106 38-39 0.010983151005113712 1106 40-41 -0.026657535896895013 1106 42-43 -0.023271254830333987 1106 44-45 -0.07609956468150614 1106 46-47 -0.07446695628812705 1106 48-49 -0.10419558948142793 1106 50-51 -0.08858708247552016 1106 52-53 -0.01444538728639344 1106 54-55 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-0.9057145896013523 2216 70-71 -0.7244287749174134 2216 72-73 -0.5226903147534046 2216 74-75 -0.39560838596173653 2216 76-77 -0.43573231724236905 2216 78-79 -0.5687834253120769 2216 80-81 -0.5103863350356832 2216 82-83 -0.4822140531560386 2216 84-85 -0.5225184840870085 2216 86-87 -0.5052769061135436 2216 88-89 -0.5563830477638412 2216 90-91 -0.4993617313510086 2216 92-93 -0.5933081097574373 2216 94-95 -0.5525376945333953 2216 96-97 -0.5975375538924297 2216 98-99 -0.6510812621707025 2216 100-101 -0.7405389524318373 2216 102-103 -0.8127259152646111 2216 104-105 -0.7778256749385832 2216 106-107 -0.8909711105729663 2216 108-109 -0.9125385706165403 2216 110-111 -0.8832144923769647 2216 112-113 -0.9396972755227964 2216 114-115 -0.9172617950852562 2216 116-117 -1.0130788137909406 2216 118-119 -1.0398398583138864 2216 120-121 -1.202177363223651 2216 122-123 -1.2706699216567898 2216 124-125 -1.6104207227373912 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48.0 3 22.0 4 49.0 5 167.0 6 563.0 7 1001.0 8 1697.0 9 3148.0 10 6026.0 11 8130.0 12 8303.0 13 10992.0 14 16457.0 15 16685.0 16 8348.0 17 7170.0 18 7075.0 19 6665.0 20 6606.0 21 6376.0 22 6716.0 23 7411.0 24 8597.0 25 10251.0 26 12550.0 27 16183.0 28 20302.0 29 26067.0 30 33274.0 31 43207.0 32 56325.0 33 76185.0 34 106660.0 35 168967.0 36 300493.0 37 555502.0 38 278692.0 39 1849.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.77891708752907 18.4944390790483 12.402262478074713 23.324381355347924 2 14.721545226675456 21.80439430340772 38.40738484088918 25.06667562902764 3 20.653184420411037 26.77104231637184 29.61826201641743 22.957511246799697 4 13.129953560329561 17.350450655072017 35.5389511583898 33.98064462620863 5 12.32480773911389 38.491044087601686 34.546572208077045 14.63757596520738 6 31.472976635767335 33.754865289749006 18.299994579064347 16.47216349541931 7 25.535747487883242 33.192628413792804 22.57340931796511 18.698214780358843 8 27.032690991072545 33.471255594904264 21.379920087122493 18.116133326900695 9 27.86824728867023 14.844649084243525 20.746449807264796 36.54065381982145 10-11 24.518473425782677 26.881574710612448 26.918137772000446 21.681814091604426 12-13 26.512732789368705 24.75680563397232 27.39815028895467 21.332311287704307 14-15 25.122951363977506 24.30874161498124 26.407743991205233 24.160563029836016 16-17 22.170589153640496 27.728936418415955 29.13107915010614 20.969395277837407 18-19 22.70947045115378 25.628849080015332 31.495089602490083 20.166590866340805 20-21 22.002677862371968 25.323064580391435 31.7309073562614 20.943350200975196 22-23 21.348133102517213 25.957896385895495 29.79552608105614 22.89844443053115 24-25 21.71405587396511 26.65836025193535 29.27531455328311 22.352269320816433 26-27 22.422495296133533 24.929326811794926 29.733938145849947 22.914239746221593 28-29 22.050838242301275 24.64034480496847 31.270554919818533 22.038262032911724 30-31 23.094344572922534 25.456631462429506 30.545914127536445 20.903109837111515 32-33 20.787403666278358 25.61437564473191 31.38756878269736 22.210651906292366 34-35 20.189206389130714 26.245718950669954 31.689115862351592 21.875958797847737 36-37 22.717710009817 25.43334928844364 30.170716066434693 21.678224635304666 38-39 23.339878000324163 24.73661871279663 30.16976743303597 21.75373585384324 40-41 21.862602537189847 25.796940790320676 30.197573393792954 22.142883278696523 42-43 23.169096884742128 25.451942503058667 29.658264304443023 21.72069630775619 44-45 23.91209366643556 24.555727875565317 29.551231353255357 21.980947104743763 46-47 22.263287508015953 25.626301321744467 31.187380031754824 20.923031138484756 48-49 23.330147732034373 25.512384002463197 30.23511472230248 20.92235354319995 50-51 21.699202985322202 26.12449647894386 30.142853348323552 22.03344718741039 52-53 21.39940772751346 26.848249554548858 28.080768273796195 23.671574444141484 54-55 21.901153484005228 27.11562865393257 27.583684372863882 23.39953348919832 56-57 22.68065909964391 25.636600770073485 27.991569630504582 23.691170499778018 58-59 22.553975885196927 25.135857854603227 29.356598883648218 22.953567376551625 60-61 23.372023120635273 26.05567990181915 28.863038478196877 21.709258499348696 62-63 21.171058116534464 26.19504769999767 29.736729838423337 22.89716434504453 64-65 20.44933782678388 26.523410375013757 30.20017790941798 22.827073888784387 66-67 22.696975594101996 26.1413279458184 28.617423739361076 22.544272720718535 68-69 23.305374848421934 25.308888586530813 28.736951547600526 22.648785017446723 70-71 21.89220922624581 26.381576129998553 28.611488004666192 23.11472663908944 72-73 23.09941298565287 26.155801381101814 28.189915322272448 22.55487031097287 74-75 23.870597731194156 25.1785192537345 28.163841455713186 22.78704155935816 76-77 22.19967486267854 26.005510746932252 30.113255986283306 21.681558404105903 78-79 22.89979341474957 25.88229682034347 29.551150041821185 21.66675972308578 80-81 21.40954455297413 26.42870965800953 29.43398026517285 22.727765523843495 82-83 21.173714290050896 26.958101302121307 27.589240654199276 24.278943753628525 84-85 21.60729396089137 27.13695935349821 27.11085838312755 24.144888302482872 86-87 22.305623661410515 25.544989887567972 27.730072058192967 24.419314392828547 88-89 22.22482719965047 25.248419983314896 28.7671451934914 23.75960762354324 90-91 22.933131102761934 26.22066080176327 28.070035164484903 22.7761729309899 92-93 20.995777768261327 26.317177474130766 28.74191154508529 23.945133212522613 94-95 20.267782558329266 26.592918363154112 29.291830894601578 23.847468183915048 96-97 22.420396356701517 26.080445513692723 27.98400500460237 23.515153125003387 98-99 22.931813284435897 25.406012704117657 27.856197085940497 23.80597692550595 100-101 21.512430621018787 26.338752107998932 27.949667138092295 24.199150132889987 102-103 22.648805256400216 25.84748232014743 27.742738663440818 23.760973760011534 104-105 23.295525709238603 24.922750018028157 27.886497966477926 23.89522630625531 106-107 21.734557338937215 25.80807900084362 29.592867413127916 22.86449624709125 108-109 22.507381164672015 25.846939680073174 28.636587533930584 23.009091621324227 110-111 20.9736681453305 26.31060181364932 28.881194367125733 23.834535673894443 112-113 20.62793750172814 26.68917362034669 27.26999181877475 25.412897059150417 114-115 20.94866622198361 27.112569888449627 26.698662154760548 25.24010173480621 116-117 21.633853960341675 25.758848910251558 27.069811632316565 25.537485497090202 118-119 21.42770003198994 25.50652540488958 28.03148026654666 25.034294296573822 120-121 22.025454052588778 26.312333965844402 27.505177554892924 24.157034426673896 122-123 20.364397763848892 26.476462815517532 27.997641877011688 25.161497543621884 124-125 19.762408644771405 27.040822411181736 28.315865480001868 24.88090346404499 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1364.0 1 1056.0 2 2244.5 3 3053.0 4 1523.5 5 884.5 6 1675.0 7 3660.5 8 5531.5 9 5745.5 10 4380.0 11 2759.0 12 2280.0 13 2402.5 14 2734.0 15 3074.0 16 3287.0 17 3608.5 18 3944.0 19 4068.5 20 4289.0 21 4715.5 22 5310.5 23 6059.0 24 6875.0 25 8037.5 26 9612.0 27 11536.0 28 13095.5 29 15015.5 30 17975.0 31 20951.5 32 24136.0 33 27130.5 34 30188.5 35 33193.5 36 36615.0 37 39502.5 38 40805.5 39 41873.0 40 42510.0 41 44904.0 42 47657.0 43 49901.5 44 53305.0 45 56748.5 46 60055.0 47 64237.0 48 69575.0 49 87929.0 50 153255.0 51 150837.5 52 77957.5 53 54587.5 54 50232.5 55 46242.0 56 41558.0 57 36826.0 58 33936.5 59 31205.5 60 27116.0 61 23019.5 62 19447.0 63 15905.5 64 12421.5 65 9468.0 66 6821.5 67 5322.5 68 4001.0 69 2676.0 70 2099.0 71 1748.5 72 1502.5 73 1329.0 74 1006.0 75 777.5 76 523.5 77 261.5 78 153.5 79 98.5 80 48.0 81 19.0 82 9.5 83 5.5 84 2.5 85 1.0 86 1.5 87 1.0 88 0.5 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14679424249996884 2 3.7945335949031824E-4 3 6.504914734119741E-4 4 0.0 5 0.0 6 0.00319824974427554 7 0.0 8 0.0 9 0.0 10-11 5.420762278433118E-5 12-13 0.005014205107550634 14-15 0.001382294381000445 16-17 5.962838506276429E-4 18-19 0.0 20-21 2.168304911373247E-4 22-23 2.710381139216559E-5 24-25 0.0 26-27 0.0 28-29 3.2524573670598706E-4 30-31 0.0 32-33 0.0 34-35 5.420762278433118E-5 36-37 0.0 38-39 0.0 40-41 8.131143417649677E-5 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 8.673219645492988E-4 96-97 0.0030898344987068775 98-99 0.004417921256922991 100-101 0.0 102-103 0.004038467897432673 104-105 0.02287561681498776 106-107 0.017509062159338968 108-109 0.01997550899602604 110-111 0.016316494458083683 112-113 0.015828625853024705 114-115 0.04060150946546406 116-117 0.03970708368952259 118-119 0.023255070174478077 120-121 0.014039774301141774 122-123 0.0038758450290796794 124-125 0.0020056820430202535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1844759.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.5180139462382 #Duplication Level Percentage of deduplicated Percentage of total 1 85.80268105987786 51.06805167946439 2 9.607268643724753 11.436110982449337 3 2.2003189788870454 3.9287584701471534 4 0.8017117280184427 1.9086515923625762 5 0.4038646422229084 1.2018610704107784 6 0.2508751252184266 0.8958953520908742 7 0.1460911105612742 0.6086536929065142 8 0.1133407145142952 0.539665138171074 9 0.08392699809543261 0.4495651418798879 >10 0.505631905299135 5.711970421997462 >50 0.0500425490907678 2.0753403297301354 >100 0.0278519636841011 3.1133340132049336 >500 0.00198942513948692 0.7073104810188132 >1k 0.0026999341178751057 4.431114518897245 >5k 8.526107740658229E-4 3.816499516438353 >10k+ 8.526107740658229E-4 8.107217598830609 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 35536 1.9263220832639927 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 19128 1.0368834086186867 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 10503 0.5693426621038303 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10453 0.5666322809646138 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 10143 0.5498279179014711 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 10094 0.5471717443850389 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 9525 0.5163276070207545 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9186 0.4979512228968662 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 8212 0.44515299830492766 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6929 0.37560461827263075 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 6177 0.33484048593881366 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5096 0.27624204570895167 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 4863 0.2636116696002025 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 4841 0.26241910189894724 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 4122 0.22344382111701314 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 3945 0.21384907188418648 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 3862 0.20934983919308703 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 3526 0.19113607793755175 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3413 0.1850106165629223 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 3114 0.1688025373504073 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 3042 0.16489958850993544 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 2875 0.15584691550495214 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2526 0.13692845515322058 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2428 0.1316161081203561 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 2037 0.11042092761168261 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.420762278433118E-5 0.0 0.0 0.0 0.0 7 5.420762278433118E-5 0.0 0.0 0.0 0.0 8 5.420762278433118E-5 0.0 0.0 0.0 0.0 9 5.420762278433118E-5 0.0 0.0 0.0 0.0 10-11 1.0841524556866235E-4 0.0 0.0 1.0841524556866235E-4 0.0 12-13 1.0841524556866235E-4 0.0 0.0 2.9814192531382147E-4 0.0 14-15 1.0841524556866235E-4 0.0 0.0 6.504914734119741E-4 0.0 16-17 1.0841524556866235E-4 0.0 0.0 0.001382294381000445 0.0 18-19 1.8972667974515912E-4 0.0 0.0 0.0022225125341575783 0.0 20-21 2.710381139216559E-4 0.0 0.0 0.003360872612628533 0.0 22-23 3.7945335949031824E-4 0.0 0.0 0.005366554655648786 0.0 24-25 3.7945335949031824E-4 0.0 0.0 0.007263821453100378 0.0 26-27 3.7945335949031824E-4 0.0 0.0 0.009079776816375472 0.0 28-29 4.60764793666815E-4 0.0 0.0 0.012196715126474515 0.0 30-31 4.878686050589806E-4 0.0 0.0 0.018593214615025593 0.0 32-33 5.420762278433118E-4 0.0 0.0 0.032904027030089024 0.0 34-35 5.691800392354774E-4 0.0 0.0 0.05393658467040952 0.0 36-37 6.233876620198085E-4 0.0 0.0 0.08659667739796906 0.0 38-39 7.046990961963053E-4 0.0 0.0 0.13671162466208323 0.0 40-41 7.589067189806365E-4 0.0 0.0 0.2032243778184576 0.0 42-43 7.589067189806365E-4 0.0 0.0 0.2605218351014956 0.0 44-45 7.589067189806365E-4 0.0 0.0 0.3292028931692432 0.0 46-47 7.589067189806365E-4 0.0 0.0 0.4173173840051736 0.0 48-49 7.589067189806365E-4 0.0 0.0 0.5026943898904952 0.0 50-51 7.589067189806365E-4 0.0 0.0 0.5932753275631126 0.0 52-53 8.131143417649677E-4 0.0 0.0 0.6948333088495571 0.0 54-55 8.131143417649677E-4 0.0 0.0 0.8105936873054963 0.0 56-57 8.673219645492988E-4 0.0 0.0 0.946085640454932 0.0 58-59 8.673219645492988E-4 0.0 0.0 1.0964575860586667 0.0 60-61 8.673219645492988E-4 0.0 0.0 1.2460164173206363 0.0 62-63 8.673219645492988E-4 0.0 0.0 1.3960089095648809 0.0 64-65 8.673219645492988E-4 0.0 0.0 1.5657329765026218 0.0 66-67 9.2152958733363E-4 0.0 0.0 1.7696078457944915 0.0 68-69 9.2152958733363E-4 0.0 0.0 2.0109130786189415 0.0 70-71 9.2152958733363E-4 0.0 0.0 2.307781124797331 0.0 72-73 9.2152958733363E-4 0.0 0.0 2.5686011018241404 0.0 74-75 9.2152958733363E-4 0.0 0.0 2.8245423928003603 0.0 76-77 9.2152958733363E-4 0.0 0.0 3.126641474577438 0.0 78-79 9.486333987257956E-4 0.0 0.0 3.453377920909994 0.0 80-81 0.0011383600784709547 0.0 0.0 3.7991412428398506 0.0 82-83 0.0011925677012552859 0.0 0.0 4.178892744255483 0.0 84-85 0.0013009829468239482 0.0 0.0 4.559891020995154 0.0 86-87 0.0013551905696082794 0.0 0.0 4.961894751563754 0.0 88-89 0.0013551905696082794 0.0 0.0 5.41452840181292 0.0 90-91 0.0014636058151769418 0.0 0.0 5.874778223063284 0.0 92-93 0.0014636058151769418 0.0 0.0 6.341289024745238 0.0 94-95 0.0014636058151769418 0.0 0.0 6.840514126777536 0.0 96-97 0.0014636058151769418 0.0 0.0 7.354402390772996 0.0 98-99 0.001517813437961273 0.0 0.0 7.929545268514749 0.0 100-101 0.0015991248721377697 0.0 0.0 8.640152995594548 0.0 102-103 0.0016804363063142665 0.0 0.0 9.292704358672324 0.0 104-105 0.0018972667974515912 0.0 0.0 9.915360217784546 0.0 106-107 0.001978578231628088 0.0 0.0 10.555687761924457 0.0 108-109 0.0020056820430202535 0.0 0.0 11.202845466535194 0.0 110-111 0.0020598896658045847 0.0 0.0 11.893071127448085 0.0 112-113 0.0020598896658045847 0.0 0.0 12.66149128422737 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1180 0.0 32.774063 5 GTTATCA 1060 0.0 30.360262 1 AAGTACT 1775 0.0 22.79342 4 AAAGTAC 1610 0.0 22.543156 3 GAGTACT 11875 0.0 21.870634 12-13 CATGGGG 5235 0.0 21.821445 4 ACCGTCC 255 6.3872176E-7 21.000837 8 AGTACTT 12305 0.0 20.647001 12-13 TAACGCA 205 5.94135E-5 20.31623 4 TTATCAA 1560 0.0 19.451645 2 TAATCGT 155 0.0056908103 19.192753 4 GTACTTT 13680 0.0 19.049131 14-15 CATGGGT 3345 0.0 18.676355 4 ACGCTAC 160 0.006770856 18.623772 1 ATGGGGA 2600 0.0 18.306934 5 ATTAGAC 275 2.8668614E-5 17.308842 3 ACTTTTT 15505 0.0 17.056393 16-17 ATAACGC 210 0.0016290682 16.999756 3 TACTTTT 14975 0.0 16.865454 14-15 CTAGACT 680 0.0 16.624311 4 >>END_MODULE