FastQCFastQC Report
Thu 26 May 2016
SRR1364157_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364157_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3391952
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA580631.7117871951018173No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT450651.328586017726666No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG288890.8516924767803318No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG136650.4028653707363783No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC135220.3986495091911678No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN125400.3696986278107709No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG106790.3148334646244994No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT92800.27358877719967734No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC63990.1886524337608551No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT60980.1797784874314259No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG58010.17102246729906556No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA56360.16615801166997646No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG50970.1502674566149521No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC49640.14634641056241363No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC46140.13602786831889133No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC43570.1284511101572192No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG42160.12429421171054307No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG37840.11155818242710983No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA36410.10734232088189927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG104700.027.0107694
CATGGGT67900.025.779094
GTATAGG12050.024.7013971
ATGGGGG50350.022.3496975
TCGCGTA1350.002548736822.0482859
ATGGGTA34850.022.0392235
GTGTAGC15050.020.5686321
ATGGGAG39200.018.9859925
AGTACTC19950.018.2052355
ATGGGGA55700.018.065125
GGGTACC14250.017.5457927
GTATCGC2050.001381760317.4235236
CTACACT12550.017.0784654
GTGTACG4201.4666512E-817.0086771
GCGCGAC2502.7078003E-416.6685039
CGGTTAC5000.016.66813328-29
GTAGCGC2150.001899210316.614853
ACCGTCC4301.9606887E-816.6131258
GCGTAAA2603.6511623E-416.0274071
TAGGACA13750.016.0209794
Produced by FastQC (version 0.11.5)