FastQCFastQC Report
Thu 26 May 2016
SRR1364157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3391952
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA535801.5796214097369303No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG300540.8860384816766275No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC160250.472441827006986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153340.4520700764633461No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG145570.42916291268272666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142220.41928659367821247No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG126710.373560710764775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99130.29225059788581914No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT95540.28166672169889195No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT75220.22176021358792813No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC74970.22102317485624798No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA64860.19121732854710208No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG63360.1867950961570211No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC63330.18670665150921947No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55000.16214852096963636No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG54700.16126407449162017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG46500.13708920409251074No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG44900.1323721562097577No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA42550.1254439921319641No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37420.11031995735788715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATCA20550.024.6422961
GAGTACT190050.021.93027912-13
CATGGGG90050.021.8693264
CGACCGT1653.216438E-421.6369886
GCGCGAC2004.9127702E-520.8249879
GTACTTT201850.020.75050214-15
AGTACTT193750.020.14494112-13
ATGGGCG6900.019.8321515
CATGGGT59800.019.7989864
CGTATAG1600.0067849318.6176191
TTATCAA29250.018.5100062
ACTTTTT231200.018.36036316-17
TGCACCG10550.018.0463185
ATGGGGA47400.017.9493525
GCACCGT11650.017.876056
GCAACGC2351.6951744E-417.7223474
TATACAG19550.017.6511235
TATCGCG1700.00969501217.4997317
CGCGGAA6200.016.79261282-83
TACTTTT235200.016.65724414-15
Produced by FastQC (version 0.11.5)