FastQCFastQC Report
Thu 26 May 2016
SRR1364156_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364156_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096803
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA862554.1136434848672No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG437022.084220596784724No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG209560.999426269420637No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC198080.9446762523708713No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG161880.7720324703846762No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT141790.676219940547586No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC102060.4867410052351127No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT92370.4405277939796919No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA84240.40175448051152163No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG80740.3850624021426906No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG80020.38162860316395963No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN76790.3662241994121527No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC76690.36574728288732894No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG63230.30155431864605303No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG55950.26683479563888457No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA55800.2661194208516489No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC43150.20578948046144535No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG31590.1506579301918206No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA29210.1393073169010155No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23520.11217076663854449No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC21830.10411087736902323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTC17350.033.964855
AAGTACT23800.026.0099754
CATGGGG70900.024.6822454
AAAGTAC24000.023.8091323
TCGACCG751.6846161E-423.80627618-19
ATGGGGG26500.023.1358415
CATGGGT48400.021.1527424
GTACAAA16900.021.1303391
ACCGTCC2306.127875E-620.7016098
CCTACAC7650.019.4519043
GTCGGGA7100.019.2797342
ATGGGAG31900.019.0328335
ATGGGGA38950.018.491495
ATGGGAT35500.018.4441365
ATGGGTG19200.017.9812875
CGGGAGT8100.017.6363934
AAAAAGT31550.017.355231
CTACACT5751.0913936E-1116.5628744
CTACACG4001.5599107E-716.368784
TGGGGGG17350.016.1227956
Produced by FastQC (version 0.11.5)