##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364156_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2096803 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06841415240249 33.0 31.0 34.0 30.0 34.0 2 31.870968803459363 33.0 31.0 34.0 30.0 34.0 3 32.11341599568486 34.0 31.0 34.0 30.0 34.0 4 35.587281685499306 37.0 35.0 37.0 33.0 37.0 5 35.721255644903216 37.0 35.0 37.0 33.0 37.0 6 35.86864526615042 37.0 35.0 37.0 35.0 37.0 7 35.84861524902435 37.0 35.0 37.0 35.0 37.0 8 35.895174701676794 37.0 35.0 37.0 35.0 37.0 9 37.54158640558985 39.0 37.0 39.0 35.0 39.0 10-11 37.5203254669132 39.0 37.0 39.0 34.5 39.0 12-13 37.49829025425851 39.0 37.0 39.0 34.5 39.0 14-15 38.76927827745382 40.0 38.0 41.0 34.0 41.0 16-17 38.748483524680196 40.0 38.0 41.0 34.0 41.0 18-19 38.849628458181336 40.0 38.0 41.0 34.5 41.0 20-21 38.92320356275721 40.0 38.5 41.0 35.0 41.0 22-23 38.914824139416055 40.0 38.0 41.0 35.0 41.0 24-25 38.89240953966586 40.0 38.0 41.0 35.0 41.0 26-27 38.710433455121915 40.0 38.0 41.0 34.0 41.0 28-29 38.56733035959983 40.0 38.0 41.0 34.0 41.0 30-31 38.42248246497167 40.0 38.0 41.0 34.0 41.0 32-33 38.14273539288145 40.0 37.0 41.0 34.0 41.0 34-35 37.97803012490921 40.0 37.0 41.0 33.5 41.0 36-37 37.895905814709344 40.0 37.0 41.0 33.0 41.0 38-39 37.79126484462298 40.0 37.0 41.0 33.0 41.0 40-41 37.582787224169365 40.0 37.0 41.0 33.0 41.0 42-43 37.404561134260106 40.0 37.0 41.0 33.0 41.0 44-45 37.139618504933466 40.0 36.0 41.0 31.5 41.0 46-47 36.75609892774858 40.0 36.0 41.0 31.0 41.0 48-49 36.37979462066775 40.0 36.0 41.0 30.0 41.0 50-51 36.192854550475175 40.0 35.5 41.0 29.5 41.0 52-53 36.09774809555309 40.0 35.5 41.0 29.0 41.0 54-55 35.92271400794448 39.0 35.0 41.0 28.0 41.0 56-57 35.648646057831854 39.0 35.0 41.0 28.0 41.0 58-59 35.50364101920877 39.0 35.0 41.0 28.0 41.0 60-61 35.34243679544525 39.0 35.0 41.0 28.0 41.0 62-63 35.00553557010363 38.0 35.0 40.0 27.5 41.0 64-65 34.742360631876245 38.0 35.0 40.0 26.0 41.0 66-67 34.3491637030279 37.0 34.0 40.0 26.0 41.0 68-69 34.02024987564401 37.0 34.0 39.5 26.0 41.0 70-71 33.62407436463988 36.0 34.0 39.0 26.0 41.0 72-73 33.30367778947283 36.0 34.0 39.0 26.0 40.0 74-75 32.92218725364281 35.0 33.0 37.5 25.0 39.5 76-77 32.46864583845025 35.0 33.0 37.0 24.5 39.0 78-79 32.095564533244186 35.0 33.0 36.5 23.5 39.0 80-81 31.824050232663726 35.0 33.0 36.0 23.0 37.5 82-83 31.639603720521194 35.0 33.0 36.0 22.5 37.0 84-85 31.383490008360347 35.0 33.0 35.0 21.0 37.0 86-87 31.08877109580633 35.0 32.0 35.0 20.0 36.0 88-89 30.902872134387444 35.0 32.0 35.0 19.5 36.0 90-91 30.721763322543893 35.0 32.0 35.0 18.0 36.0 92-93 30.508793148426435 35.0 32.0 35.0 17.5 36.0 94-95 30.43723921608277 35.0 32.0 35.0 16.0 35.0 96-97 30.372810655078233 35.0 32.0 35.0 12.5 35.0 98-99 30.24880472795966 35.0 32.0 35.0 8.5 35.0 100-101 30.070127951934445 35.0 31.0 35.0 7.0 35.0 102-103 28.721630978208253 33.5 29.0 34.5 2.0 35.0 104-105 29.555662119903488 34.0 30.5 35.0 2.0 35.0 106-107 29.649972124229123 34.0 31.0 35.0 2.0 35.0 108-109 29.62034487741576 34.5 31.0 35.0 2.0 35.0 110-111 29.576310459303997 35.0 31.0 35.0 2.0 35.0 112-113 29.546980093027337 35.0 31.0 35.0 2.0 35.0 114-115 29.417574755472977 35.0 31.0 35.0 2.0 35.0 116-117 29.173918102940526 34.0 31.0 35.0 2.0 35.0 118-119 29.01545209540429 34.0 31.0 35.0 2.0 35.0 120-121 28.8098874810843 34.0 30.5 35.0 2.0 35.0 122-123 28.47046694420029 34.0 30.0 35.0 2.0 35.0 124-125 27.426106553643812 33.5 27.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36365164681323137 1101 2 0.6482465236865664 1101 3 0.4912941907489099 1101 4 0.5338711270019019 1101 5 0.337232108176444 1101 6 0.2787015469112504 1101 7 0.2251649128489106 1101 8 0.2316246260119641 1101 9 0.31559296543402127 1101 10-11 0.29980411218625136 1101 12-13 0.28976963984826654 1101 14-15 0.40574905851609344 1101 16-17 0.42192139846947896 1101 18-19 0.3496865959145907 1101 20-21 0.24524195801706128 1101 22-23 0.22994853115422842 1101 24-25 0.2552457409877036 1101 26-27 0.273556302999296 1101 28-29 0.32076816368166305 1101 30-31 0.31127137885074774 1101 32-33 0.3063051045432985 1101 34-35 0.3045920726744811 1101 36-37 0.30286836460730626 1101 38-39 0.32936842497412755 1101 40-41 0.4539084442757826 1101 42-43 0.4561094592679211 1101 44-45 0.4537980832022086 1101 46-47 0.40259001785677384 1101 48-49 0.3494430408531599 1101 50-51 0.30539688431799306 1101 52-53 0.3350217235073387 1101 54-55 0.40900110261954836 1101 56-57 0.49890935806670456 1101 58-59 0.49162321945682663 1101 60-61 0.39174996660805306 1101 62-63 0.3950192185002166 1101 64-65 0.3797361192498556 1101 66-67 0.3808927763988734 1101 68-69 0.41682050274562243 1101 70-71 0.36677928779639757 1101 72-73 0.21155450258983421 1101 74-75 0.20099771128376887 1101 76-77 0.14923508840116284 1101 78-79 0.18891450554110634 1101 80-81 0.18396239676046022 1101 82-83 0.28345698651111917 1101 84-85 0.3210048567388384 1101 86-87 0.3321153592947077 1101 88-89 0.3019060265288722 1101 90-91 0.244483512883523 1101 92-93 0.2643457360182282 1101 94-95 0.3253882302111215 1101 96-97 0.4079052150167364 1101 98-99 0.41047617557807925 1101 100-101 0.3810392394191595 1101 102-103 0.3590073243798635 1101 104-105 0.3740015306172957 1101 106-107 0.3922014259036537 1101 108-109 0.3694714577635594 1101 110-111 0.4490695296820171 1101 112-113 0.5568713445726665 1101 114-115 0.5704829508685592 1101 116-117 0.5642191393347602 1101 118-119 0.46606686553633736 1101 120-121 0.30848624519000367 1101 122-123 0.44368091319987357 1101 124-125 0.5838999438755721 1102 1 0.3252054565894653 1102 2 0.6658573420539469 1102 3 0.4699341562379544 1102 4 0.48725496181899786 1102 5 0.30319423291448544 1102 6 0.25676111829812953 1102 7 0.2760843115618954 1102 8 0.1843410661083098 1102 9 0.348401345401669 1102 10-11 0.26128772586437066 1102 12-13 0.31750103384067074 1102 14-15 0.4408822588030077 1102 16-17 0.44680527710595186 1102 18-19 0.3585627861638869 1102 20-21 0.20085186000628852 1102 22-23 0.29648023341385965 1102 24-25 0.21972438818552575 1102 26-27 0.3318034830305834 1102 28-29 0.4008254309347947 1102 30-31 0.37736042026133987 1102 32-33 0.37175770133094943 1102 34-35 0.3409165858326304 1102 36-37 0.27113084738799387 1102 38-39 0.29161605936563006 1102 40-41 0.37958992075923703 1102 42-43 0.4563837558392123 1102 44-45 0.4340720224115344 1102 46-47 0.38249899053474934 1102 48-49 0.3402115626254343 1102 50-51 0.31244217952023945 1102 52-53 0.3007297596417331 1102 54-55 0.31084685584070115 1102 56-57 0.393237056000558 1102 58-59 0.38999001151215396 1102 60-61 0.39086554895062875 1102 62-63 0.40697781513982534 1102 64-65 0.33174416372269633 1102 66-67 0.3406003756157361 1102 68-69 0.4529234195849483 1102 70-71 0.4108841343902725 1102 72-73 0.34010701012208244 1102 74-75 0.2861888127158494 1102 76-77 0.3450320013043964 1102 78-79 0.36546412695014396 1102 80-81 0.29859012558059206 1102 82-83 0.33200524611585536 1102 84-85 0.3403237801604284 1102 86-87 0.43450599377383625 1102 88-89 0.39524801170855994 1102 90-91 0.3810664008616129 1102 92-93 0.34667390022434574 1102 94-95 0.3072433365305578 1102 96-97 0.3085760876350676 1102 98-99 0.294044938824932 1102 100-101 0.26892703031817433 1102 102-103 0.16938357333862442 1102 104-105 0.22209345176963424 1102 106-107 0.1971076439165671 1102 108-109 0.231593079523158 1102 110-111 0.20247531673343389 1102 112-113 0.15289152361100378 1102 114-115 0.10523597546952601 1102 116-117 0.19593110942866687 1102 118-119 0.22755030904209406 1102 120-121 0.19709460967197145 1102 122-123 0.3507607959341037 1102 124-125 0.31648785491626796 1103 1 0.1895771040962586 1103 2 0.5186832471291609 1103 3 0.36129409733485573 1103 4 0.44944859994792097 1103 5 0.13609041858794768 1103 6 0.1683066220119045 1103 7 0.19824850229244362 1103 8 0.14811532749206435 1103 9 0.256532354149428 1103 10-11 0.22612080451347794 1103 12-13 0.23446501661686625 1103 14-15 0.3535345828194565 1103 16-17 0.3245763108173705 1103 18-19 0.20812863631532252 1103 20-21 0.0864722465900627 1103 22-23 0.21058650456179606 1103 24-25 0.21238609900132133 1103 26-27 0.20680118666906822 1103 28-29 0.1341760315228555 1103 30-31 0.16060252360601623 1103 32-33 0.2511521199217981 1103 34-35 0.2630037641227645 1103 36-37 0.259173887585888 1103 38-39 0.17344768297780178 1103 40-41 0.13368421657647644 1103 42-43 0.17577144051207938 1103 44-45 0.23431804705946035 1103 46-47 0.22058085351343237 1103 48-49 0.2796823273226394 1103 50-51 0.3042800025300778 1103 52-53 0.2501364784270734 1103 54-55 0.3043299115988489 1103 56-57 0.2895539723643239 1103 58-59 0.19309514895809343 1103 60-61 0.11769048690734962 1103 62-63 0.08406065655014316 1103 64-65 0.15044914660403919 1103 66-67 0.1378347675449163 1103 68-69 0.19200387028831756 1103 70-71 0.1838155485846542 1103 72-73 0.18217530479576283 1103 74-75 0.3227066173797084 1103 76-77 0.21075333853512035 1103 78-79 0.225584988738035 1103 80-81 0.23034454474020905 1103 82-83 0.19164052474676652 1103 84-85 0.1870604610301143 1103 86-87 0.2418820003715858 1103 88-89 0.17360999654126275 1103 90-91 0.10750793726187524 1103 92-93 -0.011700066166472567 1103 94-95 0.12739655807407146 1103 96-97 0.11049137249754537 1103 98-99 0.14806266850026617 1103 100-101 0.1020828994497478 1103 102-103 -0.07768655479160103 1103 104-105 0.13358124804827654 1103 106-107 0.06614741814349401 1103 108-109 0.1295850683611306 1103 110-111 0.10464087126918997 1103 112-113 0.14065780683805684 1103 114-115 0.08127524641703943 1103 116-117 0.1220404751897064 1103 118-119 0.1941423746641675 1103 120-121 0.08994555105564217 1103 122-123 0.030908462618032928 1103 124-125 0.06968768548890125 1104 1 0.2079333466223119 1104 2 0.53062795106284 1104 3 0.044360239615201635 1104 4 0.409535369660162 1104 5 0.11939912879124392 1104 6 0.16882242556066984 1104 7 0.06067685055403871 1104 8 0.10642169575046978 1104 9 0.2559005785365329 1104 10-11 0.1774315871417329 1104 12-13 0.1768948302294504 1104 14-15 0.220075752640561 1104 16-17 0.23379174619670096 1104 18-19 0.12963983185855454 1104 20-21 0.1310314768621197 1104 22-23 0.07093374041264866 1104 24-25 0.11499819181037196 1104 26-27 0.23933386268781476 1104 28-29 0.2718537118722324 1104 30-31 0.2404572445220623 1104 32-33 0.23011868113552225 1104 34-35 0.21392219902323717 1104 36-37 0.22657296807814475 1104 38-39 0.21789669792176625 1104 40-41 0.20190724299727947 1104 42-43 0.3153227291051053 1104 44-45 0.23496772088802942 1104 46-47 0.21133752413131646 1104 48-49 0.202090841011902 1104 50-51 0.15506315413372818 1104 52-53 0.12875287308796857 1104 54-55 0.14534288780520654 1104 56-57 0.20081032767006946 1104 58-59 0.1901288075470049 1104 60-61 0.14276949810943762 1104 62-63 0.11987179374101942 1104 64-65 0.046669587727016904 1104 66-67 0.11132185806435757 1104 68-69 0.013660711253251634 1104 70-71 -0.004018060810331292 1104 72-73 0.012033244250602593 1104 74-75 0.0026667912331390653 1104 76-77 -0.026333910032882102 1104 78-79 -0.09146180567583428 1104 80-81 0.0029811560113799374 1104 82-83 -0.07059947357854668 1104 84-85 -0.05566985420352211 1104 86-87 0.002340759899635003 1104 88-89 0.009468181965729627 1104 90-91 -0.03548645515388671 1104 92-93 -0.02545803728335727 1104 94-95 -0.05186872540592802 1104 96-97 -0.015560527094510945 1104 98-99 -0.044017946737813674 1104 100-101 0.005458335457085184 1104 102-103 -0.08574818326755818 1104 104-105 -0.06792319641361289 1104 106-107 -0.030576423378796846 1104 108-109 -0.063831789978682 1104 110-111 5.050228857683692E-5 1104 112-113 0.0691660010284636 1104 114-115 0.010742512830933748 1104 116-117 -0.019622645850901677 1104 118-119 0.04650747733031935 1104 120-121 0.014423349679784536 1104 122-123 -0.030089546074638207 1104 124-125 -0.08161451436927081 1105 1 0.2207600166415915 1105 2 0.5287326782441326 1105 3 0.1488090494383556 1105 4 0.4092162430152868 1105 5 0.17623038769661292 1105 6 0.1110091659847754 1105 7 0.16158111256057595 1105 8 0.12497357740330273 1105 9 0.14262863988930974 1105 10-11 0.10330389457615041 1105 12-13 0.13934496014312714 1105 14-15 0.22094198346781724 1105 16-17 0.2822973199122316 1105 18-19 0.18098564319038246 1105 20-21 0.1707491765520075 1105 22-23 0.17770255694102133 1105 24-25 0.1437256664562483 1105 26-27 0.13538239172980582 1105 28-29 0.2589150436968879 1105 30-31 0.16377040980577817 1105 32-33 0.11350680870236829 1105 34-35 0.1361686578280299 1105 36-37 0.1412741666365207 1105 38-39 0.1241468026908592 1105 40-41 0.107516946710291 1105 42-43 0.10307150330676507 1105 44-45 0.13709463816235967 1105 46-47 0.10316914972849389 1105 48-49 0.10687323561419504 1105 50-51 0.18303498172571153 1105 52-53 0.04884616996165647 1105 54-55 0.05943768287686879 1105 56-57 0.06887440444486259 1105 58-59 0.14244614264045907 1105 60-61 0.12012538822761343 1105 62-63 0.20984450139334854 1105 64-65 0.26299680254555824 1105 66-67 0.19667435366406494 1105 68-69 0.1632686129379337 1105 70-71 0.21396047200334323 1105 72-73 0.20476651262709566 1105 74-75 0.1517591766157551 1105 76-77 0.10466412030824301 1105 78-79 0.2079086368687939 1105 80-81 0.14072301545316535 1105 82-83 0.12531819887137274 1105 84-85 0.14498081422326692 1105 86-87 0.08165761305528108 1105 88-89 0.17196687235211883 1105 90-91 0.20356218913410373 1105 92-93 0.16280342445497098 1105 94-95 0.11655649041839666 1105 96-97 0.1593413566537052 1105 98-99 0.15944975295206731 1105 100-101 0.1883238224238113 1105 102-103 0.059421123580147395 1105 104-105 0.21146081372803494 1105 106-107 0.21669633246731834 1105 108-109 0.13922212419448243 1105 110-111 0.2942968982319165 1105 112-113 0.34111475846706796 1105 114-115 0.33196825878684777 1105 116-117 0.29925291990217673 1105 118-119 0.18328509446216046 1105 120-121 0.12582976992955253 1105 122-123 0.18560596352221026 1105 124-125 0.2168087108423613 1106 1 0.1754985958023525 1106 2 0.43847793329921103 1106 3 0.33007529030592764 1106 4 0.4158770390299935 1106 5 0.11864080176528802 1106 6 0.07820914248040367 1106 7 0.05982697644414969 1106 8 0.014848661464078816 1106 9 0.16738453720132895 1106 10-11 0.16114382654405546 1106 12-13 0.10045461129907807 1106 14-15 0.20459420365154557 1106 16-17 0.1564248833577082 1106 18-19 0.06667248090575839 1106 20-21 0.08864924461674661 1106 22-23 0.018920940297313393 1106 24-25 0.06579765838641549 1106 26-27 0.1512682453628642 1106 28-29 0.17922726825504753 1106 30-31 0.03610505084179749 1106 32-33 -0.002879614585239665 1106 34-35 0.08119087272327619 1106 36-37 0.06390234707173903 1106 38-39 0.01908063135704907 1106 40-41 0.08263750862799668 1106 42-43 0.08148084090788643 1106 44-45 0.08146334176570491 1106 46-47 0.06449497172858543 1106 48-49 -0.1413784227497743 1106 50-51 -0.17755747124922294 1106 52-53 -0.0648771387029754 1106 54-55 -0.012651366044870826 1106 56-57 0.10678622061455911 1106 58-59 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-1.2885700713613737 2216 104-105 -1.2447063702380596 2216 106-107 -1.2944223034738656 2216 108-109 -1.3951857302507555 2216 110-111 -1.3921523303181722 2216 112-113 -1.4128973619188692 2216 114-115 -1.429881753703551 2216 116-117 -1.5208977001856034 2216 118-119 -1.6362875929340852 2216 120-121 -1.782874828148266 2216 122-123 -1.82803215676703 2216 124-125 -2.128170350954214 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18.0 3 14.0 4 52.0 5 202.0 6 606.0 7 1171.0 8 2080.0 9 3941.0 10 7271.0 11 10030.0 12 11659.0 13 15904.0 14 23567.0 15 23860.0 16 12413.0 17 10560.0 18 10235.0 19 9978.0 20 10206.0 21 9828.0 22 10438.0 23 11383.0 24 12493.0 25 14795.0 26 17714.0 27 21539.0 28 27123.0 29 33936.0 30 42877.0 31 54521.0 32 70621.0 33 93669.0 34 132170.0 35 206845.0 36 338896.0 37 563242.0 38 278664.0 39 2282.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.54790333365173 18.834941255134204 12.310870188174611 23.30628522303945 2 12.95224823326189 22.9153253383009 37.37916121789277 26.75326521054444 3 22.12977972614447 27.693832360660224 29.400767550919284 20.77562036227602 4 13.24316113626316 18.851699468190382 34.57005736828877 33.33508202725769 5 11.36673306934414 39.38238356202275 35.08188418272961 14.168999185903491 6 31.608030206993455 32.06249913555994 18.453851777980315 17.875618879466295 7 23.39452013374647 34.20493007688371 22.908732961561004 19.49181682780881 8 27.72225144660705 33.06214270010106 22.124396044835876 17.091209808456014 9 28.631969717708337 14.720982371734493 21.0639244602378 35.58312345031936 10-11 23.874720616138436 27.84040358612868 25.71807584075078 22.5667999569821 12-13 27.115851144427435 25.906571911574527 26.283282304818016 20.69429463918003 14-15 27.17502292811184 23.776320828550183 26.09056973794468 22.958086505393293 16-17 21.74793166228895 28.007373166395816 30.85848981241587 19.386205358899367 18-19 23.171299354302715 25.61485270671589 32.97124241046966 18.24260552851174 20-21 20.89023750476917 25.610835558946967 33.1372806180847 20.36164631819916 22-23 19.525534826113848 26.874746936169014 29.56093157058627 24.038786667130864 24-25 20.893331419308346 27.806665671500852 28.307666480828193 22.99233642836261 26-27 22.32951784216257 24.684102416869873 29.485697988795323 23.500681752172238 28-29 21.747850770555864 23.771197797785003 32.44897696392309 22.031974467736042 30-31 23.57584379648446 25.78518344355669 30.75868357685486 19.88028918310399 32-33 19.165343620740718 25.93400524512794 32.50305345805018 22.39759767608116 34-35 17.85951551911698 27.01062237188937 33.67105414390779 21.45880796508586 36-37 22.806219754550142 25.64375384812021 30.47525208615211 21.074774311177542 38-39 23.96856070885057 24.13753223359562 30.608431025709137 21.28547603184467 40-41 21.179768890745688 26.278823378762073 30.37556505733378 22.165842673158462 42-43 23.51222313207297 25.629184048286845 29.830198640501752 21.02839417913843 44-45 25.2968924596159 23.46679683308351 29.63423364045168 21.602077066848913 46-47 21.840392254303335 25.75640153128358 33.22303048975035 19.18017572466274 48-49 23.58054142425397 25.545222894091623 31.785413317321655 19.08882236433275 50-51 20.496346104045063 26.462834133678747 31.335728726065348 21.705091036210842 52-53 19.63563100586941 27.81973318428102 27.760786301812807 24.78384950803676 54-55 21.08560031629104 28.298223533636683 26.44869355871772 24.167482591354553 56-57 22.666340137819336 25.37100528757351 27.38077921483325 24.581875359773903 58-59 22.25578654742482 24.29875386481229 30.303776749651735 23.141682838111162 60-61 23.69342756567975 26.3574355816927 28.88564161726209 21.06349523536546 62-63 19.53218781163514 26.515080338973192 30.624860799989317 23.32787104940235 64-65 18.117152636656854 27.239564231832937 31.869159859080703 22.774123272429502 66-67 22.725120099503865 26.326436007579158 28.635427362513312 22.31301653040367 68-69 24.023525338336505 24.770877378561554 28.69144120835386 22.514156074748083 70-71 21.199368753287743 26.74049493443113 28.64839949198852 23.41173682029261 72-73 23.501230206175784 26.124271092706376 28.249339589842247 22.125159111275593 74-75 24.97134923977121 24.016300052985425 28.256946408413192 22.75540429883017 76-77 21.65007871507242 26.075697144653077 31.99706887103843 20.27715526923607 78-79 23.250014426724878 25.74438323485802 30.88752257603599 20.11807976238111 80-81 20.190189540934462 26.56491811581727 30.61224635790773 22.63264598534054 82-83 19.41448481330864 27.582634133964902 27.34687044991828 25.656010602808184 84-85 20.79737581451381 27.922294082944365 26.200840040766828 25.079490061774994 86-87 22.11361773137486 25.17506413334968 27.20043323097115 25.510884904304316 88-89 21.912525878682928 24.256355985755455 29.68545447521775 24.145663660343867 90-91 23.098450355135892 26.24118717876691 28.23610515627839 22.424257309818802 92-93 19.452089681290992 26.358532489699794 29.55091632356497 24.63846150544424 94-95 18.11697639525711 27.033902427624806 30.828073686248736 24.02104749086934 96-97 22.475695557285228 26.019131640797887 27.961960876333503 23.543211925583382 98-99 23.383509549840095 24.672814868814747 28.03474776322244 23.90892781812272 100-101 20.95165831029429 26.369382340639536 27.957418984997638 24.721540364068538 102-103 22.931378206809715 25.70109838747371 27.72929971240658 23.638223693309996 104-105 24.2545652467387 23.785840663217144 27.83985990735224 24.119734182691918 106-107 21.275635659413915 25.749614227895318 31.142267028231124 21.832483084459646 108-109 22.68792479263763 25.69535589862671 29.805372615454996 21.811346693280665 110-111 20.007498340062124 26.277722870509585 29.823192766486805 23.891586022941485 112-113 19.114719512436526 27.170397032529277 26.891522898898167 26.823360556136027 114-115 20.229634983909328 27.683014978995057 25.903498915758362 26.183851121337256 116-117 21.577250597824737 25.217301897198958 26.60401196222015 26.601435542756157 118-119 21.152087131132937 24.521158240729235 28.819875835655047 25.506878792482784 120-121 22.324853494058196 26.22086694885099 27.504147384548165 23.950132172542645 122-123 19.163636354964858 26.48481696392695 28.437355951393872 25.91419072971432 124-125 17.97019977937421 27.37925077846185 29.382314548663825 25.268234893500125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1905.0 1 1387.5 2 1408.5 3 1870.0 4 1230.0 5 878.0 6 1920.5 7 4286.5 8 6487.0 9 6854.0 10 5309.0 11 3464.5 12 2866.0 13 2953.0 14 3254.0 15 3615.0 16 3902.5 17 4327.0 18 4770.5 19 4941.5 20 5224.5 21 5884.5 22 6610.5 23 7426.5 24 8761.5 25 10382.5 26 12507.0 27 14861.0 28 16521.0 29 18427.5 30 21920.0 31 25110.0 32 27750.5 33 31437.0 34 35518.0 35 37990.5 36 40868.0 37 44060.0 38 44614.5 39 44671.0 40 44142.5 41 44207.0 42 44913.5 43 45919.0 44 47812.0 45 50314.5 46 55330.5 47 65506.5 48 77544.0 49 120796.5 50 278382.0 51 273611.5 52 103753.5 53 50319.5 54 38904.5 55 33473.0 56 29097.0 57 25117.5 58 23637.0 59 21977.5 60 19030.5 61 16336.5 62 13954.5 63 10728.0 64 7826.5 65 6361.5 66 4653.5 67 3325.0 68 2539.5 69 1850.5 70 1564.5 71 1377.0 72 1255.0 73 1291.5 74 961.0 75 688.5 76 496.5 77 276.0 78 154.5 79 66.0 80 36.5 81 21.5 82 11.5 83 5.0 84 2.0 85 3.0 86 2.5 87 1.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14321803240457018 2 4.7691652482374365E-5 3 1.9076660992949746E-4 4 0.0 5 0.0 6 0.003338415673766205 7 0.0 8 0.0 9 0.0 10-11 7.153747872356153E-5 12-13 0.004745319421996249 14-15 0.0015261328794359797 16-17 5.007623510649308E-4 18-19 0.0 20-21 1.4307495744712306E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.3845826241187176E-4 30-31 0.0 32-33 0.0 34-35 9.538330496474873E-5 36-37 0.0 38-39 0.0 40-41 1.1922913120593588E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0010015247021298616 96-97 0.0031953407163190822 98-99 0.004411477854619628 100-101 0.0 102-103 0.004435323680860815 104-105 0.022701226581610195 106-107 0.01707361158869002 108-109 0.01967280664897942 110-111 0.016167470191524907 112-113 0.016095932712801346 114-115 0.041396354354700945 116-117 0.04153942931214807 118-119 0.022343539187992388 120-121 0.014355187397194682 122-123 0.0034099531524897663 124-125 0.001907666099294974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2096803.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.86807565028888 #Duplication Level Percentage of deduplicated Percentage of total 1 82.81212082155295 39.640568642469596 2 10.777378491800029 10.3178473791456 3 3.050932130132818 4.381267500272842 4 1.2143001454459326 2.325048448974508 5 0.5792258525962504 1.3863213465340194 6 0.35315496402034846 1.0142909120400654 7 0.22072121661109412 0.7395849926054556 8 0.1450958707908082 0.555636809564716 9 0.10987108532413041 0.4733385681667337 >10 0.6220890350321379 5.756307676774684 >50 0.06241413504994725 2.0666796930057036 >100 0.04209643407455841 3.8084191938213614 >500 0.005299908784467828 1.776237465919805 >1k 0.002574241409598659 3.037036261357842 >5k 0.001211407722164075 4.71280993916237 >10k+ 0.0015142596527050935 18.008605170184772 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 78084 3.723954992433719 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 44348 2.115029404288338 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 23487 1.1201338418535265 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 21985 1.0485009798250002 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 18958 0.9041383477608531 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 13951 0.6653462437816047 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13144 0.6268590802283286 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12068 0.5755428621572937 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 11441 0.545640196050845 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 10981 0.5237020359089528 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 9724 0.4637536287386082 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 9390 0.4478246168094952 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 9049 0.4315617633130055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8543 0.40742978715692413 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8388 0.40003758102215614 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 6821 0.3253047615822755 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 6553 0.3125233987169992 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 6550 0.31238032375955205 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4856 0.23159066445440987 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 4641 0.2213369591706994 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 4297 0.20493103071676264 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3692 0.17607758096492612 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3275 0.15619016187977602 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 2825 0.13472891826270755 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2645 0.12614442081588018 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2377 0.11336305795060385 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2131 0.10163091143993974 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.538330496474872E-5 0.0 0.0 0.0 0.0 8 9.538330496474872E-5 0.0 0.0 0.0 0.0 9 9.538330496474872E-5 0.0 0.0 0.0 0.0 10-11 9.538330496474872E-5 4.769165248237436E-5 0.0 2.6230408865305895E-4 0.0 12-13 9.538330496474872E-5 4.769165248237436E-5 0.0 8.584497446827385E-4 0.0 14-15 9.538330496474872E-5 4.769165248237436E-5 0.0 0.0014069037482300436 0.0 16-17 9.538330496474872E-5 4.769165248237436E-5 0.0 0.0027184241914953384 0.0 18-19 1.1922913120593589E-4 4.769165248237436E-5 0.0 0.004387632028378441 0.0 20-21 1.4307495744712308E-4 4.769165248237436E-5 0.0 0.00667683134753241 0.0 22-23 1.4307495744712308E-4 4.769165248237436E-5 0.0 0.010325242762434048 0.0 24-25 1.4307495744712308E-4 4.769165248237436E-5 0.0 0.013782887567406189 0.0 26-27 1.4307495744712308E-4 4.769165248237436E-5 0.0 0.01776514054968445 0.0 28-29 2.6230408865305895E-4 4.769165248237436E-5 0.0 0.024227359461046174 0.0 30-31 3.338415673766205E-4 4.769165248237436E-5 0.0 0.037962555375969986 0.0 32-33 3.338415673766205E-4 9.538330496474872E-5 0.0 0.06221376066325735 0.0 34-35 3.338415673766205E-4 9.538330496474872E-5 0.0 0.10067707839029227 0.0 36-37 3.8153321985899487E-4 9.538330496474872E-5 0.0 0.15547478709254042 0.0 38-39 4.292248723413692E-4 9.538330496474872E-5 0.0 0.24432433566720382 0.0 40-41 4.292248723413692E-4 9.538330496474872E-5 0.0 0.36720187828804135 0.0 42-43 4.5307069858255636E-4 1.1922913120593589E-4 0.0 0.465375144923009 0.0 44-45 4.7691652482374357E-4 1.4307495744712308E-4 0.0 0.573158279533175 0.0 46-47 5.246081773061179E-4 1.4307495744712308E-4 0.0 0.7121794465192963 0.0 48-49 5.722998297884923E-4 1.4307495744712308E-4 0.0 0.8437607157181671 0.0 50-51 5.722998297884923E-4 1.4307495744712308E-4 0.0 0.9802065334702402 0.0 52-53 6.199914822708667E-4 1.4307495744712308E-4 0.0 1.13828051562307 0.0 54-55 6.67683134753241E-4 1.4307495744712308E-4 0.0 1.3055828325312393 0.0 56-57 6.67683134753241E-4 1.4307495744712308E-4 0.0 1.5049577857338052 0.0 58-59 6.67683134753241E-4 1.9076660992949743E-4 0.0 1.730801606064089 0.0 60-61 7.392206134768026E-4 1.9076660992949743E-4 0.0 1.9411933309900835 4.769165248237436E-5 62-63 7.630664397179897E-4 1.9076660992949743E-4 0.0 2.1436443957777627 4.769165248237436E-5 64-65 7.630664397179897E-4 1.9076660992949743E-4 0.0 2.3808626752250923 4.769165248237436E-5 66-67 7.630664397179897E-4 1.9076660992949743E-4 0.0 2.654088152296615 4.769165248237436E-5 68-69 7.630664397179897E-4 1.9076660992949743E-4 0.0 2.9862128201838702 4.769165248237436E-5 70-71 7.630664397179897E-4 1.9076660992949743E-4 0.0 3.427336759819592 4.769165248237436E-5 72-73 7.869122659591768E-4 1.9076660992949743E-4 0.0 3.797376291430335 4.769165248237436E-5 74-75 8.107580922003641E-4 1.9076660992949743E-4 0.0 4.135700874140298 4.769165248237436E-5 76-77 8.107580922003641E-4 1.9076660992949743E-4 0.0 4.539339174924874 4.769165248237436E-5 78-79 8.107580922003641E-4 1.9076660992949743E-4 0.0 4.954685776393871 4.769165248237436E-5 80-81 8.107580922003641E-4 1.9076660992949743E-4 0.0 5.383242965600488 4.769165248237436E-5 82-83 8.107580922003641E-4 1.9076660992949743E-4 0.0 5.887629882254079 4.769165248237436E-5 84-85 8.107580922003641E-4 1.9076660992949743E-4 0.0 6.362471820194839 4.769165248237436E-5 86-87 8.107580922003641E-4 1.9076660992949743E-4 0.0 6.842440610777455 4.769165248237436E-5 88-89 8.107580922003641E-4 1.9076660992949743E-4 0.0 7.4082782216545855 4.769165248237436E-5 90-91 9.061413971651128E-4 1.9076660992949743E-4 0.0 7.956994529290544 4.769165248237436E-5 92-93 9.061413971651128E-4 1.9076660992949743E-4 0.0 8.512983813930063 4.769165248237436E-5 94-95 9.061413971651128E-4 1.9076660992949743E-4 0.0 9.106673349856901 4.769165248237436E-5 96-97 9.061413971651128E-4 1.9076660992949743E-4 0.0 9.697286774198625 4.769165248237436E-5 98-99 9.776788758886744E-4 1.9076660992949743E-4 0.0 10.38170967897318 4.769165248237436E-5 100-101 0.0010253705283710486 1.9076660992949743E-4 0.0 11.307142349567414 4.769165248237436E-5 102-103 0.0010492163546122358 1.9076660992949743E-4 0.0 12.10530984551243 4.769165248237436E-5 104-105 0.001192291312059359 2.1461243617068462E-4 0.0 12.830270654897003 4.769165248237436E-5 106-107 0.0014069037482300436 2.3845826241187179E-4 0.0 13.593122482178822 4.769165248237436E-5 108-109 0.0014307495744712308 2.3845826241187179E-4 0.0 14.294642844368308 4.769165248237436E-5 110-111 0.0014307495744712308 2.3845826241187179E-4 0.0 15.042829488511796 4.769165248237436E-5 112-113 0.0014307495744712308 2.3845826241187179E-4 0.0 15.917995157389605 4.769165248237436E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTATCA 1305 0.0 36.070686 1 AGTACTC 1610 0.0 33.627945 5 GCGTATC 100 4.4028522E-4 29.79256 1 TAAAGCG 85 0.007214307 27.99793 5 AAAGTAC 1970 0.0 27.180084 3 TTATCAA 1810 0.0 25.638376 2 ATAGTAC 515 0.0 24.259764 3 AAGTACT 2250 0.0 24.062666 4 GTGTTAT 775 0.0 23.065208 1 AGAGCGT 130 0.0020524652 22.885105 8 TACACCG 165 3.2181077E-4 21.634764 5 GAGTACT 16140 0.0 21.308388 12-13 CGTACAC 200 4.9152983E-5 20.822966 3 CGTGTAC 385 2.3101165E-10 20.088945 3 GTATAGG 450 3.6379788E-12 19.861706 1 GTACTTT 17360 0.0 19.826862 14-15 AGTACTT 16525 0.0 19.353668 12-13 CCAAGAC 955 0.0 19.312248 3 TAGACTG 895 0.0 18.61315 5 ATCGCTA 80 0.006834762 18.594149 12-13 >>END_MODULE