##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364155_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1936975 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.27494521096039 31.0 31.0 34.0 26.0 34.0 2 30.356653544831502 31.0 31.0 34.0 26.0 34.0 3 30.52561803843622 33.0 31.0 34.0 26.0 34.0 4 33.79385020457156 37.0 35.0 37.0 28.0 37.0 5 33.91935363130655 37.0 35.0 37.0 30.0 37.0 6 33.74642470863073 37.0 35.0 37.0 28.0 37.0 7 33.69336878379948 37.0 35.0 37.0 28.0 37.0 8 33.57692484415132 37.0 35.0 37.0 27.0 37.0 9 34.94325120355193 39.0 35.0 39.0 27.0 39.0 10-11 34.965328153435124 39.0 35.0 39.0 27.0 39.0 12-13 34.85959550329767 39.0 35.0 39.0 26.5 39.0 14-15 36.02908039597827 40.0 36.0 41.0 27.0 41.0 16-17 36.060043624724116 40.0 36.0 41.0 27.0 41.0 18-19 35.97950747944604 40.0 36.0 41.0 27.0 41.0 20-21 35.9902040552924 40.0 36.0 41.0 27.0 41.0 22-23 35.99327043456937 40.0 36.0 41.0 27.0 41.0 24-25 35.96374940306406 40.0 36.0 41.0 27.0 41.0 26-27 35.71061345654952 40.0 36.0 41.0 25.0 41.0 28-29 35.53263180990979 40.0 36.0 41.0 25.0 41.0 30-31 35.54924069747932 40.0 35.5 41.0 25.0 41.0 32-33 35.168866144374604 39.0 35.0 41.0 24.5 41.0 34-35 35.18780882561727 39.0 35.0 41.0 25.0 41.0 36-37 35.179743930613455 39.5 35.0 41.0 25.0 41.0 38-39 34.994665909472246 39.5 35.0 41.0 24.5 41.0 40-41 34.59177118961267 39.0 34.0 41.0 23.5 41.0 42-43 34.297837349475344 39.0 33.0 41.0 21.5 41.0 44-45 34.027966545773694 38.0 33.0 40.5 18.5 41.0 46-47 33.729656810232456 38.0 33.0 40.0 16.0 41.0 48-49 33.38929697079209 38.0 32.5 40.0 14.5 41.0 50-51 33.27094283612334 38.0 32.5 40.0 11.0 41.0 52-53 33.10293602137354 38.0 32.0 40.0 8.5 41.0 54-55 32.757407555595705 38.0 32.0 40.0 7.5 41.0 56-57 32.31557557531718 37.0 31.0 40.0 3.5 41.0 58-59 31.882359090850425 37.0 31.0 40.0 2.0 41.0 60-61 31.602619548522824 36.0 30.0 40.0 2.0 41.0 62-63 30.9330739942436 36.0 28.5 39.0 2.0 41.0 64-65 30.66894539165451 35.5 28.5 39.0 2.0 41.0 66-67 30.24210637721189 35.0 28.5 38.5 2.0 40.5 68-69 29.79031247176654 35.0 28.0 38.0 2.0 40.0 70-71 29.081215555182695 34.0 26.5 37.0 2.0 39.5 72-73 28.754521870442314 34.0 26.5 37.0 2.0 39.0 74-75 28.38720453284116 34.0 26.0 36.0 2.0 39.0 76-77 27.81080834806851 34.0 26.0 36.0 2.0 38.5 78-79 27.426305966778095 33.5 26.0 35.0 2.0 37.0 80-81 27.19254068844461 34.0 25.5 35.0 2.0 37.0 82-83 27.01717162069722 34.0 25.0 35.0 2.0 36.5 84-85 26.734479278256046 33.5 24.5 35.0 2.0 36.0 86-87 26.29671110881658 33.0 23.5 35.0 2.0 36.0 88-89 25.95281069709212 33.0 21.5 35.0 2.0 35.5 90-91 25.81881722789401 33.0 20.0 35.0 2.0 35.0 92-93 25.37588817615096 32.0 19.0 35.0 2.0 35.0 94-95 25.302285780663148 32.0 19.0 35.0 2.0 35.0 96-97 25.249925786342104 32.5 18.5 35.0 2.0 35.0 98-99 24.9625199086204 32.0 17.5 35.0 2.0 35.0 100-101 24.324071296738474 32.0 8.5 35.0 2.0 35.0 102-103 23.00004052711057 29.5 7.0 34.0 2.0 35.0 104-105 23.68262161359852 31.0 7.0 35.0 2.0 35.0 106-107 23.723989984382865 31.0 7.0 35.0 2.0 35.0 108-109 23.740152557467184 32.0 2.0 35.0 2.0 35.0 110-111 23.780689219014185 32.0 2.0 35.0 2.0 35.0 112-113 23.677100375585642 32.0 2.0 35.0 2.0 35.0 114-115 23.47806760541566 32.0 2.0 35.0 2.0 35.0 116-117 23.006564875643722 31.5 2.0 35.0 2.0 35.0 118-119 22.68357361349527 31.0 2.0 35.0 2.0 35.0 120-121 22.468102840769756 31.5 2.0 35.0 2.0 35.0 122-123 21.920150234257022 31.0 2.0 35.0 2.0 35.0 124-125 20.90122872004027 29.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.44845613640298865 1101 2 0.5135676648190071 1101 3 0.4763322114035482 1101 4 0.5301414285191157 1101 5 0.3922803184298047 1101 6 0.4790284662850155 1101 7 0.4863928743541308 1101 8 0.44021052466059984 1101 9 0.6158073714278842 1101 10-11 0.5516450177897383 1101 12-13 0.5143619056702704 1101 14-15 0.5510932526363632 1101 16-17 0.5971272086778043 1101 18-19 0.6500776740116052 1101 20-21 0.5956167846608054 1101 22-23 0.5806546548895497 1101 24-25 0.7826363774669716 1101 26-27 0.7941150926641427 1101 28-29 0.8352347216782121 1101 30-31 0.8113557278700299 1101 32-33 0.8601201572893515 1101 34-35 0.7694786351856777 1101 36-37 0.8058186484334442 1101 38-39 0.900914644163521 1101 40-41 0.9223181171579071 1101 42-43 1.027280388542131 1101 44-45 1.009408634176907 1101 46-47 0.9751946908556235 1101 48-49 0.9947465521193735 1101 50-51 1.0650278125054626 1101 52-53 1.007723363542155 1101 54-55 0.9411424200506673 1101 56-57 0.9205859763098374 1101 58-59 0.9366964811820111 1101 60-61 0.9498396374579876 1101 62-63 0.9819707720723798 1101 64-65 0.8706611446804438 1101 66-67 0.8711825642711482 1101 68-69 1.0776615204407243 1101 70-71 1.011413771691295 1101 72-73 1.003255664014862 1101 74-75 0.8983121082274756 1101 76-77 0.9282482306777879 1101 78-79 1.0153372782813435 1101 80-81 1.021314410477487 1101 82-83 0.8760698587170879 1101 84-85 0.8693947135877309 1101 86-87 0.9037711978762779 1101 88-89 0.8741710841861767 1101 90-91 0.7887122081717237 1101 92-93 0.6679132271691799 1101 94-95 0.6528771240064195 1101 96-97 0.7333257551247705 1101 98-99 0.6483322722306823 1101 100-101 0.5069922827621305 1101 102-103 0.5111252152064765 1101 104-105 0.42307740149861317 1101 106-107 0.3893845754122971 1101 108-109 0.48893853425276035 1101 110-111 0.3751677741932511 1101 112-113 0.4383190512332291 1101 114-115 0.45684554374718545 1101 116-117 0.4622438317881006 1101 118-119 0.4124753298615431 1101 120-121 0.4861098665476078 1101 122-123 0.46496872074673234 1101 124-125 0.2831297633751966 1102 1 0.19818873331880482 1102 2 0.3872251631878143 1102 3 0.3469354035649239 1102 4 0.42720325269900883 1102 5 0.38551103793084707 1102 6 0.31690829389025765 1102 7 0.2714250012346824 1102 8 0.3073345731622439 1102 9 0.4623154948701256 1102 10-11 0.4766461700467133 1102 12-13 0.42773070284479786 1102 14-15 0.4677559765669628 1102 16-17 0.4171353777824294 1102 18-19 0.4730425284011659 1102 20-21 0.5110590942410127 1102 22-23 0.5618459088862338 1102 24-25 0.5999094491664536 1102 26-27 0.7317282323791758 1102 28-29 0.693180447379433 1102 30-31 0.6692888612669137 1102 32-33 0.8159297880548664 1102 34-35 0.6649019927365813 1102 36-37 0.5833845168825036 1102 38-39 0.6712505127067132 1102 40-41 0.7341840778241888 1102 42-43 0.8049265176494416 1102 44-45 0.8716719309473149 1102 46-47 0.9150449818050959 1102 48-49 0.8053048080925578 1102 50-51 0.7272498236333931 1102 52-53 0.8180939575040327 1102 54-55 0.839716359922285 1102 56-57 0.8176193149857269 1102 58-59 0.8058066906189367 1102 60-61 0.827674418845195 1102 62-63 0.8588312391930799 1102 64-65 0.761783619021795 1102 66-67 0.8193837535959574 1102 68-69 0.8560481503965427 1102 70-71 0.9893255861946706 1102 72-73 0.8803795914836776 1102 74-75 1.0489754842682828 1102 76-77 0.8539728291574953 1102 78-79 0.6765944499348713 1102 80-81 0.7161663219220458 1102 82-83 0.8398477285124137 1102 84-85 0.9324702995669476 1102 86-87 0.8827954141000767 1102 88-89 0.8130683944332908 1102 90-91 0.8072030290542642 1102 92-93 0.6812797748446826 1102 94-95 0.743481321107037 1102 96-97 0.87781657918595 1102 98-99 0.6809866847513923 1102 100-101 0.5829996151509178 1102 102-103 0.4575039435524495 1102 104-105 0.6719037803956738 1102 106-107 0.5680782375343618 1102 108-109 0.5027887242192826 1102 110-111 0.45997963355212335 1102 112-113 0.5603429426244801 1102 114-115 0.6053322238399375 1102 116-117 0.49585856033408504 1102 118-119 0.42624776540514375 1102 120-121 0.4060532525253322 1102 122-123 0.28150355332226695 1102 124-125 0.21459321286704025 1103 1 0.15695756231755098 1103 2 0.12581863200031052 1103 3 0.1924833170927016 1103 4 0.2213174753068614 1103 5 0.15278478690130726 1103 6 0.1422336524550829 1103 7 0.218307902306222 1103 8 0.3661984498565545 1103 9 0.37661186251169454 1103 10-11 0.36419133206157284 1103 12-13 0.36316001556367894 1103 14-15 0.33542422792031346 1103 16-17 0.31163147840973693 1103 18-19 0.25782585064779795 1103 20-21 0.34117393286993547 1103 22-23 0.3461354450120808 1103 24-25 0.3477766198024952 1103 26-27 0.5679040219134563 1103 28-29 0.6146149053341432 1103 30-31 0.4428111914922539 1103 32-33 0.49544285139913313 1103 34-35 0.4497808054289578 1103 36-37 0.4557039187820351 1103 38-39 0.5632144412506577 1103 40-41 0.5107997360794414 1103 42-43 0.6052828597526982 1103 44-45 0.5978336326492837 1103 46-47 0.5995561562813378 1103 48-49 0.5775492655482708 1103 50-51 0.6898739896326447 1103 52-53 0.7357988434438099 1103 54-55 0.7546183978535339 1103 56-57 0.7332210178747474 1103 58-59 0.8173435040405295 1103 60-61 0.7027924805758197 1103 62-63 0.7430838954249595 1103 64-65 0.6899243063639382 1103 66-67 0.6398711689005374 1103 68-69 0.6419349975688711 1103 70-71 0.6847472620711379 1103 72-73 0.7656174267128755 1103 74-75 0.7041128762327489 1103 76-77 0.6622388435215072 1103 78-79 0.6365956202313079 1103 80-81 0.6005035793383229 1103 82-83 0.664072314382441 1103 84-85 0.5880652200017487 1103 86-87 0.583573709097827 1103 88-89 0.5431653674938595 1103 90-91 0.42578953831246835 1103 92-93 0.4436013031417474 1103 94-95 0.5739652578310945 1103 96-97 0.37236877745857555 1103 98-99 0.397347692244999 1103 100-101 0.31183187828525405 1103 102-103 0.26596602445200546 1103 104-105 0.33250841919707597 1103 106-107 0.28813908098304 1103 108-109 0.3573858532595473 1103 110-111 0.4508467741529536 1103 112-113 0.4443554966265424 1103 114-115 0.3830426118482535 1103 116-117 0.309578838184958 1103 118-119 0.3852303111158051 1103 120-121 0.35416927269894316 1103 122-123 0.40606838672793444 1103 124-125 0.36278283726769445 1104 1 0.2370023083893038 1104 2 0.2731640108645017 1104 3 0.2136726328498959 1104 4 0.2894431792224239 1104 5 0.1739737457938233 1104 6 0.25032950820943256 1104 7 0.31808571836103994 1104 8 0.23860850449491267 1104 9 0.4034463015703835 1104 10-11 0.3697649039962201 1104 12-13 0.37418094829374127 1104 14-15 0.42077668174559335 1104 16-17 0.39023389642959216 1104 18-19 0.3283899513940227 1104 20-21 0.37869337604685427 1104 22-23 0.37005192059753966 1104 24-25 0.44696953465886935 1104 26-27 0.48777999240693504 1104 28-29 0.5708045327531508 1104 30-31 0.5291126079595188 1104 32-33 0.5281667471051961 1104 34-35 0.47719235414075456 1104 36-37 0.6048603263824006 1104 38-39 0.6146918134134083 1104 40-41 0.7253654531847218 1104 42-43 0.6729941843058924 1104 44-45 0.7466893222855049 1104 46-47 0.7347601068208505 1104 48-49 0.8394456467977491 1104 50-51 0.8193930859370937 1104 52-53 0.8734669445478573 1104 54-55 0.9524700606287837 1104 56-57 0.8350027480213029 1104 58-59 0.9052654429862201 1104 60-61 0.8063339699005674 1104 62-63 0.8070470703214809 1104 64-65 0.8975583435454304 1104 66-67 0.8662959154195597 1104 68-69 0.8631951390680932 1104 70-71 0.7589366915492093 1104 72-73 0.6692049634758881 1104 74-75 0.6060313569218501 1104 76-77 0.4548999334242829 1104 78-79 0.6312076179264388 1104 80-81 0.4248741771151998 1104 82-83 0.5363421212044557 1104 84-85 0.4797917051178757 1104 86-87 0.4169920753523222 1104 88-89 0.44664828343033847 1104 90-91 0.38981115574687664 1104 92-93 0.37788812336807354 1104 94-95 0.352536200386826 1104 96-97 0.361022384147077 1104 98-99 0.31655471990962525 1104 100-101 0.30270256622993585 1104 102-103 0.18446461626400534 1104 104-105 0.3340789211438917 1104 106-107 0.23486828540797333 1104 108-109 0.31995984914941644 1104 110-111 0.3520181586498943 1104 112-113 0.408851981985336 1104 114-115 0.5078971300167083 1104 116-117 0.22622720562424448 1104 118-119 0.3358705144430232 1104 120-121 0.32950831792892643 1104 122-123 0.36316129811082476 1104 124-125 0.25091313760024647 1105 1 0.27952976117223116 1105 2 0.3464211833823434 1105 3 0.3226165218428285 1105 4 0.3822459916650871 1105 5 0.31127711294859495 1105 6 0.3176481240054656 1105 7 0.32056148357379044 1105 8 0.3234987076231661 1105 9 0.36600608641624177 1105 10-11 0.4056967728008587 1105 12-13 0.4881502817931249 1105 14-15 0.5918455934356146 1105 16-17 0.5354016267301773 1105 18-19 0.46754628038821267 1105 20-21 0.5574722869097997 1105 22-23 0.5602040932912544 1105 24-25 0.5866450447135563 1105 26-27 0.6560703525847842 1105 28-29 0.7370898249443059 1105 30-31 0.5628930066107145 1105 32-33 0.4362680155754006 1105 34-35 0.5422059676409319 1105 36-37 0.6229831796655674 1105 38-39 0.752783042877887 1105 40-41 0.730569860080081 1105 42-43 0.6909221515878627 1105 44-45 0.7480108632728815 1105 46-47 0.7830246945133652 1105 48-49 0.8222254484323201 1105 50-51 0.6939534041051303 1105 52-53 0.7208608671467402 1105 54-55 0.7116502607814681 1105 56-57 0.5861378401453337 1105 58-59 0.6099542100423108 1105 60-61 0.5665644154670098 1105 62-63 0.6172467619262676 1105 64-65 0.6467641830905535 1105 66-67 0.5472816725801941 1105 68-69 0.5522036636669831 1105 70-71 0.7231803389785689 1105 72-73 0.6604113268365559 1105 74-75 0.7276818131927563 1105 76-77 0.5100953327837274 1105 78-79 0.4640340496017821 1105 80-81 0.44974828551174184 1105 82-83 0.4756665037247956 1105 84-85 0.4277249390751905 1105 86-87 0.2775809685614483 1105 88-89 0.38519609276908895 1105 90-91 0.34378027059367255 1105 92-93 0.33828705025920414 1105 94-95 0.33427989772601663 1105 96-97 0.3000632313022642 1105 98-99 0.20415374338815795 1105 100-101 0.1518448776919925 1105 102-103 0.0759251157235532 1105 104-105 0.07402577430536894 1105 106-107 0.1489476819648594 1105 108-109 0.24767912679732262 1105 110-111 0.22427715834282935 1105 112-113 0.4451868132473322 1105 114-115 0.45795248239436503 1105 116-117 0.22663325773771348 1105 118-119 0.17060920558864012 1105 120-121 0.20010864761899683 1105 122-123 0.16195255333828484 1105 124-125 0.30448824999242774 1106 1 0.029512036735411584 1106 2 0.07329874660858948 1106 3 0.09268603799297281 1106 4 0.035316954856433824 1106 5 0.005137004456862826 1106 6 0.02436277693372091 1106 7 0.19453664754755806 1106 8 0.06091776602836774 1106 9 0.10591565543065684 1106 10-11 0.1227039834653354 1106 12-13 0.18893895106058523 1106 14-15 0.1980192874627491 1106 16-17 0.20861436723715343 1106 18-19 0.33551513264458066 1106 20-21 0.2577368354911158 1106 22-23 0.17337541769753528 1106 24-25 0.2657138936977219 1106 26-27 0.3602741572487602 1106 28-29 0.47512435305617373 1106 30-31 0.3329428668149035 1106 32-33 0.03440856308780127 1106 34-35 0.40258023957559885 1106 36-37 0.4087208707259009 1106 38-39 0.24950731699403406 1106 40-41 0.473291706402847 1106 42-43 0.4572718083700451 1106 44-45 0.47001659235623094 1106 46-47 0.42978223603399357 1106 48-49 0.4837342661123998 1106 50-51 0.46204721364105694 1106 52-53 0.5051667418431549 1106 54-55 0.4793961714357593 1106 56-57 0.4718006795455736 1106 58-59 0.6310856017469106 1106 60-61 0.5236832082783458 1106 62-63 0.612825310267624 1106 64-65 0.7642423184070886 1106 66-67 0.5725026372258526 1106 68-69 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2216 76-77 -3.3129182023481825 2216 78-79 -3.388651996636426 2216 80-81 -3.415400472828626 2216 82-83 -3.5196619176197323 2216 84-85 -3.6174401412088244 2216 86-87 -3.6616127979642137 2216 88-89 -3.6177604858336814 2216 90-91 -3.753791688258488 2216 92-93 -3.86394389916223 2216 94-95 -3.7403777914662335 2216 96-97 -3.744984583154615 2216 98-99 -3.7549048745251135 2216 100-101 -3.6723280384223003 2216 102-103 -3.5178719375696588 2216 104-105 -3.735975004088367 2216 106-107 -3.764207800562229 2216 108-109 -3.72509189003625 2216 110-111 -3.8709474568995788 2216 112-113 -3.9880128147453284 2216 114-115 -4.055308488700469 2216 116-117 -3.8167306721452263 2216 118-119 -3.796425312930239 2216 120-121 -3.8394194633410272 2216 122-123 -3.7211592621110476 2216 124-125 -3.6894525426232114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 65722.0 3 12781.0 4 8330.0 5 7177.0 6 9493.0 7 8728.0 8 8997.0 9 10051.0 10 12023.0 11 14044.0 12 15795.0 13 17023.0 14 17709.0 15 17940.0 16 17759.0 17 18234.0 18 18616.0 19 19581.0 20 20393.0 21 21386.0 22 23470.0 23 27681.0 24 31281.0 25 32303.0 26 37594.0 27 40611.0 28 46480.0 29 56500.0 30 67482.0 31 80992.0 32 98481.0 33 116960.0 34 134809.0 35 153627.0 36 200499.0 37 305529.0 38 140053.0 39 841.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.174643696252254 16.82343011321103 9.526057524256357 23.475868666280356 2 11.750035531662602 23.47143824908266 36.023711289939406 28.75481492931533 3 23.165405613979402 31.65415986611007 25.56054997767291 19.619884542237614 4 13.775766873392891 19.474896714681503 31.510242439141273 35.23909397278433 5 11.132151313110882 42.19340215917077 33.151699510646736 13.522747017071609 6 32.39635693867908 27.97821922789051 20.68535363411394 18.940070199316473 7 25.231051351717177 33.476554660155756 21.95709998335279 19.335294004774276 8 29.542103925342246 33.163461688099474 22.055889569597404 15.23854481696088 9 31.578180074978494 16.36644831661553 18.59966816879604 33.455703439609934 10-11 23.378168425645683 30.59943815400723 23.677146866251963 22.345246554095123 12-13 27.55996552867362 27.59228888258217 23.003540003912008 21.844205584832203 14-15 29.024996181027348 25.24196984814105 22.09785958577698 23.63517438505463 16-17 23.43272294191305 28.9606625881689 29.719571290889153 17.8870431790289 18-19 26.12032009775434 28.23164895361933 27.971003556094253 17.67702739253207 20-21 21.19085619793901 29.347758994598806 28.83339289706402 20.627991910398165 22-23 19.747904936094294 31.602593217925296 21.740183801700226 26.909318044280184 24-25 21.062709740736523 33.32491279687254 20.440298958438426 25.172078503952513 26-27 24.479733934205157 27.12726704911982 21.71783838661039 26.67516063006464 28-29 23.557137273200514 27.27728045778397 25.6947645186299 23.470817750385624 30-31 26.785357600710984 29.547344681191053 23.768840101874424 19.89845761622354 32-33 19.228577291037457 30.099067893841635 26.76882402822492 23.90353078689599 34-35 16.86711829780523 31.79978994664624 28.310538644966517 23.022553110582013 36-37 24.93772168177387 28.894495032254046 24.437394992843956 21.730388293128126 38-39 27.51240529188785 27.088710082898913 23.538228203462854 21.86065642175038 40-41 21.939604938770717 31.30246433183464 23.64360160292617 23.11432912646847 42-43 25.953584546912566 29.247976561600957 22.818797164749924 21.97964172673655 44-45 28.351360762127385 26.27279852465379 23.09654178786428 22.279298925354542 46-47 23.11608552040505 28.41268882316229 30.242068578015967 18.229157078416694 48-49 25.741425967857733 28.110856927430085 27.96298788293169 18.18472922178049 50-51 20.328049899671793 29.642188658505603 28.83772236826414 21.192039073558462 52-53 19.035545166825273 31.68030165914083 22.172133495472185 27.112019678561715 54-55 20.62505897614305 32.92929706659146 20.782104019917604 25.663539937347895 56-57 24.036632522171544 27.003214702358164 22.01149703839422 26.94865573707607 58-59 23.40319590212864 26.843836046783792 25.733546604292428 24.019421446795143 60-61 26.110806519555425 29.126625838436865 24.21461391400149 20.54795372800622 62-63 19.100010369141433 29.76389464952302 26.815092285358773 24.321002695976773 64-65 16.71123046644596 31.1723801061805 28.44057985743905 23.675809569934486 66-67 24.32650191628092 28.49192424468668 24.726686285733244 22.454887553299155 68-69 26.796497921120267 26.71828392742612 23.881011031880302 22.604207119573307 70-71 21.561096466768145 30.25558418742823 24.061921588423356 24.12139775738027 72-73 25.307794700170135 28.455848838228302 23.450214858668264 22.7861416029333 74-75 27.631479328480264 25.68917165611734 23.634957856297596 23.044391159104794 76-77 22.708555378193328 27.616050333653863 30.396166487043125 19.279227801109684 78-79 25.139149669854106 27.426308548693118 28.265056174444993 19.16948560700779 80-81 19.98732234430335 29.021253869407182 28.996520774244395 21.994903012045068 82-83 18.89319478197657 30.661254169425543 22.67350850840211 27.77204254019578 84-85 20.31735036349128 31.93201279439527 21.517900798327542 26.23273604378591 86-87 23.40466021169393 26.290549478128263 22.846543419656022 27.458246890521785 88-89 22.959111592009222 25.976585941973724 26.321204625573902 24.743097840443156 90-91 25.394358485655122 28.344373573522503 24.78501085185788 21.476257088964495 92-93 19.060797115314315 28.8046744435785 27.111777940596177 25.02275050051101 94-95 16.978404956010095 29.97022401341929 28.757225246199575 24.294145784371043 96-97 24.034581594789277 27.520654538348353 25.36465688857396 23.080106978288402 98-99 26.141188608959016 26.040784895343055 24.54846182806272 23.269564667635205 100-101 21.42905817610185 28.804201234532396 24.740085147134156 25.0266554422316 102-103 24.648204579797504 27.44762024669137 24.44401440677988 23.46016076673125 104-105 26.46946664753059 25.078016639059253 24.61426905287308 23.83824766053708 106-107 22.082209868936207 26.883235536005877 30.636569653583436 20.39798494147448 108-109 24.26508238250025 27.12019884573286 28.44339260837839 20.171326163388503 110-111 19.501861759407348 28.681902330134378 29.353441941796792 22.462793968661483 112-113 18.613151644182288 30.164606120605765 23.32801621311329 27.89422602209866 114-115 19.74322426152355 31.7916249850983 22.332725171175184 26.132425582202963 116-117 22.42732264024477 26.636045400242043 23.536082631819085 27.4005493276941 118-119 21.966307079848395 26.271444402325844 26.569616426322728 25.192632091503032 120-121 24.138121485359832 28.512526879615365 25.191421237703054 22.15793039732175 122-123 18.844863959859218 29.078560654361112 26.90829406855656 25.168281317223112 124-125 17.32833189456446 30.1763867576594 28.17844604150237 24.316835306273767 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9677.0 1 6326.0 2 5144.5 3 7208.0 4 6211.5 5 4595.5 6 3326.5 7 2587.5 8 2302.0 9 2083.0 10 1786.5 11 1574.0 12 1460.0 13 1362.0 14 1386.0 15 1467.5 16 1512.5 17 1602.0 18 1748.0 19 1906.5 20 2171.0 21 2577.5 22 3106.5 23 3746.0 24 4403.5 25 5240.5 26 6486.5 27 8022.5 28 9413.0 29 10931.5 30 13055.5 31 15202.5 32 17438.0 33 19735.5 34 21955.0 35 23799.0 36 26027.0 37 28371.0 38 29377.5 39 29711.5 40 30145.0 41 31691.5 42 32736.0 43 34042.0 44 36497.5 45 38727.5 46 44242.0 47 58221.0 48 78018.0 49 134665.5 50 317924.5 51 329707.5 52 138111.5 53 57833.5 54 39017.0 55 31177.5 56 26284.5 57 22296.5 58 19769.0 59 17911.5 60 15562.0 61 13121.5 62 11057.5 63 8887.5 64 6848.0 65 5220.5 66 3751.5 67 2874.5 68 2151.0 69 1461.0 70 1191.0 71 1004.0 72 905.5 73 849.0 74 599.5 75 428.0 76 307.0 77 170.5 78 117.5 79 68.0 80 34.0 81 23.0 82 21.0 83 17.0 84 16.0 85 12.0 86 8.0 87 8.5 88 8.5 89 8.0 90 4.5 91 2.0 92 5.0 93 4.0 94 0.5 95 2.0 96 3.5 97 1.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4819886678971076 2 0.47068237845093513 3 0.45555569896359016 4 0.45540081828624523 5 0.4605118806386247 6 0.46650060016262473 7 0.4503930097187625 8 0.47729062068431444 9 0.4898875824416939 10-11 0.4945081893158146 12-13 0.4943791220846939 14-15 0.4698305347255386 16-17 0.47192142386969377 18-19 0.45826611081712465 20-21 0.46815266072096956 22-23 0.48573161759960765 24-25 0.4723086255630558 26-27 0.48175634688109037 28-29 0.47772944927012484 30-31 0.49066198582841797 32-33 0.4883129622220215 34-35 0.4837181687941249 36-37 0.4406355270460383 38-39 0.4005214316137276 40-41 0.39383574904167584 42-43 0.4043934485473483 44-45 0.4377186076227107 46-47 0.44484311878057275 48-49 0.4429329237599866 50-51 0.42876134178293474 52-53 0.42207565921088297 54-55 0.4248376979568657 56-57 0.40627783012171037 58-59 0.41164702693633115 60-61 0.42140450960905534 62-63 0.42204984576465876 64-65 0.4370990849133314 66-67 0.4253539668813485 68-69 0.4280901921811071 70-71 0.43735721937557276 72-73 0.43872533202545205 74-75 0.44259734895907277 76-77 0.43389821758153824 78-79 0.42367609287677954 80-81 0.4328914931787968 82-83 0.43118780572800375 84-85 0.4294324913847623 86-87 0.43552446469365896 88-89 0.4221272861033312 90-91 0.42710928122458985 92-93 0.4360407336181417 94-95 0.434053098258883 96-97 0.4026123207578828 98-99 0.37509518708295153 100-101 0.374630545050917 102-103 0.3568192671562617 104-105 0.358187379806141 106-107 0.37708282244221014 108-109 0.38405245292272744 110-111 0.40018585681281377 112-113 0.39610733230939993 114-115 0.3957201306160379 116-117 0.3697259902683308 118-119 0.4418229455723486 120-121 0.44923140463867633 122-123 0.40088281986086555 124-125 0.39564269027736554 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1936975.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.69218908751656 #Duplication Level Percentage of deduplicated Percentage of total 1 87.03727166739202 44.12109833024984 2 9.131235430813014 9.25764626122808 3 2.047831876969947 3.1142724218041344 4 0.7128835792323257 1.4455051678332256 5 0.3217552452763276 0.8155238866723934 6 0.18300019512050314 0.5566008296460582 7 0.11648758600406471 0.41335075152464773 8 0.07414092915566056 0.3006692799906332 9 0.05170417812717326 0.23588981768135742 >10 0.2842393171718673 2.5954411345499957 >50 0.018579553770774398 0.6501565467537248 >100 0.013654616839451319 1.4552684683938706 >500 0.002331266256369556 0.8058545214758323 >1k 0.0021092408986200743 1.8821092507513415 >5k 9.991141098726667E-4 3.9651193843007775 >10k+ 0.0017762028619958522 28.38549394714407 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 120182 6.2046231882187435 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 94693 4.8887053266046285 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 58139 3.001535900050336 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 34049 1.75784406097136 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 29253 1.5102414847894268 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 25260 1.3040953032434597 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 21459 1.1078614850475612 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 20329 1.0495230965810092 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 18677 0.9642354702564566 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 13418 0.6927296428709715 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 12474 0.6439938563997987 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 12271 0.6335135972327985 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 11461 0.5916958143496948 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 11287 0.5827127350636947 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 11113 0.5737296557776946 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 10344 0.5340285754849701 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 9501 0.4905071051510732 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 9072 0.46835916829076263 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 8744 0.45142554756772807 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 7952 0.4105370487486932 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 7799 0.4026381342041069 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 7452 0.38472360252455506 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 7137 0.368461131403348 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 6420 0.3314446495179339 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 6383 0.3295344544973477 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 4312 0.22261516023696745 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3549 0.1832238412989326 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 3525 0.181984795880174 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 2867 0.14801430064920817 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.162689244827631E-5 0.0 0.0 0.0 0.0 3 5.162689244827631E-5 0.0 0.0 0.0 0.0 4 5.162689244827631E-5 0.0 0.0 0.0 0.0 5 5.162689244827631E-5 0.0 0.0 0.0 0.0 6 5.162689244827631E-5 0.0 0.0 0.0 0.0 7 1.0325378489655261E-4 0.0 0.0 0.0 0.0 8 1.0325378489655261E-4 0.0 0.0 0.0 0.0 9 1.0325378489655261E-4 0.0 0.0 0.0 0.0 10-11 1.0325378489655261E-4 0.0 0.0 3.0976135468965783E-4 0.0 12-13 1.0325378489655261E-4 0.0 0.0 9.292840640689735E-4 0.0 14-15 1.0325378489655261E-4 0.0 0.0 0.0021425160366034665 0.0 16-17 1.0325378489655261E-4 0.0 0.0 0.004104337949637967 0.0 18-19 1.5488067734482892E-4 0.0 0.0 0.006091973308896605 0.0 20-21 2.0650756979310523E-4 0.0 0.0 0.009112146517120768 0.0 22-23 2.0650756979310523E-4 0.0 0.0 0.013706939945017359 0.0 24-25 2.0650756979310523E-4 0.0 0.0 0.018095225803120847 0.0 26-27 2.0650756979310523E-4 0.0 0.0 0.022457698215000196 0.0 28-29 2.0650756979310523E-4 0.0 0.0 0.03056312032937957 0.0 30-31 2.0650756979310523E-4 0.0 0.0 0.04135314085106932 0.0 32-33 2.0650756979310523E-4 0.0 0.0 0.057822119542069464 0.0 34-35 2.0650756979310523E-4 0.0 0.0 0.08554576078679385 0.0 36-37 2.0650756979310523E-4 0.0 0.0 0.12297525781179416 0.0 38-39 2.0650756979310523E-4 0.0 0.0 0.18053924289162226 0.0 40-41 2.0650756979310523E-4 0.0 0.0 0.28399953535796796 0.0 42-43 2.0650756979310523E-4 0.0 0.0 0.35382490739426165 0.0 44-45 2.0650756979310523E-4 0.0 0.0 0.42370190632300364 0.0 46-47 4.1301513958621046E-4 0.0 0.0 0.5192632842447631 0.0 48-49 4.6464203203448675E-4 0.0 0.0 0.5966261825785051 0.0 50-51 4.6464203203448675E-4 0.0 0.0 0.6824042643813162 0.0 52-53 4.6464203203448675E-4 0.0 0.0 0.7944604344402999 0.0 54-55 4.6464203203448675E-4 0.0 0.0 0.9105176886640252 0.0 56-57 4.6464203203448675E-4 0.0 0.0 1.0343189767549918 0.0 58-59 4.6464203203448675E-4 0.0 0.0 1.1920907600769242 0.0 60-61 4.6464203203448675E-4 0.0 0.0 1.3306831528543217 0.0 62-63 5.162689244827631E-4 0.0 0.0 1.4641902967255644 0.0 64-65 5.162689244827631E-4 0.0 0.0 1.625730843196221 0.0 66-67 5.678958169310394E-4 0.0 0.0 1.8024755094928948 0.0 68-69 5.678958169310394E-4 0.0 0.0 2.033815614553621 0.0 70-71 6.195227093793157E-4 0.0 0.0 2.402663947650331 0.0 72-73 6.195227093793157E-4 0.0 0.0 2.677473896152506 0.0 74-75 6.195227093793157E-4 0.0 0.0 2.922598381496922 0.0 76-77 6.195227093793157E-4 0.0 0.0 3.212870584287355 0.0 78-79 6.195227093793157E-4 0.0 0.0 3.4831373662540814 0.0 80-81 6.71149601827592E-4 0.0 0.0 3.781953819744705 0.0 82-83 6.71149601827592E-4 0.0 0.0 4.169413647569019 0.0 84-85 7.227764942758683E-4 0.0 0.0 4.534622284748125 0.0 86-87 8.260302791724209E-4 0.0 0.0 4.891260857780818 0.0 88-89 8.260302791724209E-4 0.0 0.0 5.321854954245667 0.0 90-91 8.776571716206973E-4 0.0 0.0 5.7057008995986 0.0 92-93 8.776571716206973E-4 0.0 0.0 6.100414305811897 0.0 94-95 8.776571716206973E-4 0.0 0.0 6.549206881864763 0.0 96-97 8.776571716206973E-4 0.0 0.0 6.9914686560229224 0.0 98-99 8.776571716206973E-4 0.0 0.0 7.5170562345926 0.0 100-101 8.776571716206973E-4 0.0 0.0 8.354341176318744 0.0 102-103 8.776571716206973E-4 0.0 0.0 8.98958427444856 0.0 104-105 0.0011357916338620789 0.0 0.0 9.555544082912789 0.0 106-107 0.001161605080086217 0.0 0.0 10.156868312704088 0.0 108-109 0.001187418526310355 0.0 0.0 10.687721834303488 0.0 110-111 0.0012390454187586313 0.0 0.0 11.244104854218563 0.0 112-113 0.0012906723112069078 0.0 0.0 11.967629938434932 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 2015 0.0 48.18047 5 GTACAAA 1430 0.0 41.222427 1 AAGTACT 2330 0.0 40.8978 4 GCGCGAC 100 1.0574398E-5 35.72517 9 AAAAAGT 2835 0.0 32.97471 1 AAAGTAC 2935 0.0 32.46742 3 GGCGCGA 115 2.751651E-5 31.065367 8 AAAAGTA 3120 0.0 30.154224 2 TATAACG 110 7.7189447E-4 27.06593 2 GAACAAA 1885 0.0 25.27045 1 GTACATA 1135 0.0 23.607555 1 TACAAAA 2235 0.0 23.178608 2 GTATAGG 350 6.366463E-11 22.116156 1 CATGGGG 6660 0.0 22.088112 4 ATGGGGG 2415 0.0 21.949839 5 GTACAAG 1065 0.0 20.686474 1 ATAACGC 175 4.7540033E-4 20.419687 3 GTCCAAG 570 0.0 19.847834 1 AGAACAA 1930 0.0 18.819939 2 CGCCTAT 80 0.006809802 18.605894 114-115 >>END_MODULE