##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364155_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1936975 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07590185727746 33.0 31.0 34.0 30.0 34.0 2 31.885007292298557 33.0 31.0 34.0 30.0 34.0 3 32.13680042334052 34.0 31.0 34.0 30.0 34.0 4 35.57777204145639 37.0 35.0 37.0 33.0 37.0 5 35.70510047883943 37.0 35.0 37.0 33.0 37.0 6 35.866414383252234 37.0 35.0 37.0 35.0 37.0 7 35.8471843983531 37.0 35.0 37.0 35.0 37.0 8 35.907936860310535 37.0 35.0 37.0 35.0 37.0 9 37.57045083183831 39.0 37.0 39.0 35.0 39.0 10-11 37.54513635952968 39.0 37.0 39.0 35.0 39.0 12-13 37.52700705997754 39.0 37.0 39.0 34.5 39.0 14-15 38.816870377779786 40.0 38.0 41.0 34.0 41.0 16-17 38.778814130280466 40.0 38.0 41.0 34.0 41.0 18-19 38.83470101575911 40.0 38.0 41.0 34.5 41.0 20-21 38.89022470604938 40.0 38.0 41.0 35.0 41.0 22-23 38.890989816595464 40.0 38.0 41.0 35.0 41.0 24-25 38.87202725899921 40.0 38.0 41.0 35.0 41.0 26-27 38.657940603260236 40.0 38.0 41.0 34.0 41.0 28-29 38.50375792150131 40.0 38.0 41.0 34.0 41.0 30-31 38.375003033079935 40.0 38.0 41.0 34.0 41.0 32-33 38.063254043031016 40.0 37.0 41.0 34.0 41.0 34-35 37.88494327495192 40.0 37.0 41.0 33.0 41.0 36-37 37.83087288168407 40.0 37.0 41.0 33.0 41.0 38-39 37.73275313310704 40.0 37.0 41.0 33.0 41.0 40-41 37.4804860671924 40.0 37.0 41.0 33.0 41.0 42-43 37.30169671782031 40.0 36.5 41.0 32.0 41.0 44-45 37.06427857871165 40.0 36.0 41.0 31.0 41.0 46-47 36.76929516385085 40.0 36.0 41.0 31.0 41.0 48-49 36.459795557505906 39.5 35.5 41.0 30.0 41.0 50-51 36.33776636249823 39.0 35.5 41.0 30.0 41.0 52-53 36.27798835813575 39.0 35.0 41.0 30.0 41.0 54-55 36.064188231649865 39.0 35.0 41.0 29.0 41.0 56-57 35.75735928445127 39.0 35.0 41.0 28.0 41.0 58-59 35.5747250222641 39.0 35.0 41.0 28.0 41.0 60-61 35.39688405890628 39.0 35.0 40.0 28.0 41.0 62-63 34.98440790407723 38.0 34.0 40.0 28.0 41.0 64-65 34.69968662476283 38.0 34.0 40.0 27.0 41.0 66-67 34.211645478129554 37.0 34.0 39.5 26.0 41.0 68-69 33.88217994553363 36.5 34.0 39.0 26.0 41.0 70-71 33.35276449102338 36.0 33.0 39.0 26.0 40.5 72-73 32.97535332154519 35.0 33.0 38.0 26.0 39.0 74-75 32.63392222408653 35.0 33.0 37.0 25.0 39.0 76-77 32.07535203087288 35.0 32.5 36.5 23.5 38.5 78-79 31.630675666954915 35.0 32.0 36.0 22.0 37.0 80-81 31.412218794770197 35.0 32.0 36.0 21.5 37.0 82-83 31.23376347139225 35.0 32.0 35.0 21.0 36.5 84-85 30.97302107667884 35.0 32.0 35.0 20.0 36.0 86-87 30.659294776649155 35.0 31.0 35.0 19.5 36.0 88-89 30.42808141560939 34.0 31.0 35.0 18.0 35.5 90-91 30.245759237987066 34.0 31.0 35.0 17.0 35.0 92-93 29.976203100194894 34.0 31.0 35.0 10.5 35.0 94-95 29.894989868222357 34.0 31.0 35.0 8.5 35.0 96-97 29.80030279172421 34.0 31.0 35.0 7.0 35.0 98-99 29.652824894487537 34.0 30.5 35.0 4.5 35.0 100-101 29.36174705404045 34.0 30.0 35.0 2.0 35.0 102-103 27.826344170678507 32.5 26.0 34.5 2.0 35.0 104-105 28.752968417248546 34.0 29.0 35.0 2.0 35.0 106-107 28.793290827191885 34.0 29.0 35.0 2.0 35.0 108-109 28.687015320280334 34.0 29.0 35.0 2.0 35.0 110-111 28.694489345500074 34.0 29.0 35.0 2.0 35.0 112-113 28.6442153357684 34.0 29.0 35.0 2.0 35.0 114-115 28.5262744743737 34.0 29.0 35.0 2.0 35.0 116-117 28.213628208934033 34.0 29.0 35.0 2.0 35.0 118-119 27.994069102595542 34.0 29.0 35.0 2.0 35.0 120-121 27.763370461673485 34.0 29.0 35.0 2.0 35.0 122-123 27.329034448043988 34.0 28.0 35.0 2.0 35.0 124-125 26.178047729062065 33.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31156151479208916 1101 2 0.5814677804503638 1101 3 0.5026165675900884 1101 4 0.5224535510816395 1101 5 0.24825183439752152 1101 6 0.2558954157566049 1101 7 0.24220247625788005 1101 8 0.1835169557632952 1101 9 0.30105281023664077 1101 10-11 0.2925022205481014 1101 12-13 0.27437643698159064 1101 14-15 0.45221889251136105 1101 16-17 0.43889120589928154 1101 18-19 0.40713956109466665 1101 20-21 0.3243450344076848 1101 22-23 0.23884207860719897 1101 24-25 0.29751837080127075 1101 26-27 0.36605016144204683 1101 28-29 0.4426269565712033 1101 30-31 0.43556422061211464 1101 32-33 0.4691000158005494 1101 34-35 0.5076536778542433 1101 36-37 0.5032139558984312 1101 38-39 0.5501142409261774 1101 40-41 0.6190628311741051 1101 42-43 0.6786683363082915 1101 44-45 0.7180386983839568 1101 46-47 0.7539490139582625 1101 48-49 0.7298108409991144 1101 50-51 0.6168776199629349 1101 52-53 0.7386971393779476 1101 54-55 0.7370958108432077 1101 56-57 0.7158124641206385 1101 58-59 0.7127061763049767 1101 60-61 0.5619511892615563 1101 62-63 0.6331618117004183 1101 64-65 0.5429831386669974 1101 66-67 0.5650883068975929 1101 68-69 0.6576065711817023 1101 70-71 0.6344585063519261 1101 72-73 0.6351230057680795 1101 74-75 0.6173640645790002 1101 76-77 0.6471373924954804 1101 78-79 0.6622250008546153 1101 80-81 0.5466897576623708 1101 82-83 0.5349587840066334 1101 84-85 0.5445526901874302 1101 86-87 0.6186643899652324 1101 88-89 0.5884616296231329 1101 90-91 0.5725748906857895 1101 92-93 0.4798965575736531 1101 94-95 0.45213124093780976 1101 96-97 0.5238775517030945 1101 98-99 0.5482008259122431 1101 100-101 0.4369318239883988 1101 102-103 0.43422433050889 1101 104-105 0.5887342054657694 1101 106-107 0.4967579104032751 1101 108-109 0.6027963360464206 1101 110-111 0.4937443113714153 1101 112-113 0.6570077286269473 1101 114-115 0.7401851872366763 1101 116-117 0.6343376232872053 1101 118-119 0.631558543953151 1101 120-121 0.5487495819091599 1101 122-123 0.4628333946947265 1101 124-125 0.45465115891694197 1102 1 0.2890942764837092 1102 2 0.6234159248520648 1102 3 0.40337930669258526 1102 4 0.4851053876814504 1102 5 0.2992917447892154 1102 6 0.24863821402826147 1102 7 0.25730871801863486 1102 8 0.2657807684514282 1102 9 0.29117579792833226 1102 10-11 0.3093571421646715 1102 12-13 0.25709918414804633 1102 14-15 0.3889240415046018 1102 16-17 0.3330605899974515 1102 18-19 0.2618077422610128 1102 20-21 0.24318121451872088 1102 22-23 0.22425077651525527 1102 24-25 0.3060359032622131 1102 26-27 0.2952466293862557 1102 28-29 0.37789102024963483 1102 30-31 0.319844550246863 1102 32-33 0.34985798449803696 1102 34-35 0.3059462958664412 1102 36-37 0.25949253385806514 1102 38-39 0.3927452183127258 1102 40-41 0.401581876423144 1102 42-43 0.3257329417981225 1102 44-45 0.44734566074237847 1102 46-47 0.44617121213692457 1102 48-49 0.42721990714037616 1102 50-51 0.5199714024331996 1102 52-53 0.47603817528936077 1102 54-55 0.5311187818959411 1102 56-57 0.45242974817086434 1102 58-59 0.5719700595344648 1102 60-61 0.6294437399354962 1102 62-63 0.6229506143055232 1102 64-65 0.6120686769678372 1102 66-67 0.5537535214148335 1102 68-69 0.5271813510700554 1102 70-71 0.38350188855195455 1102 72-73 0.24468925712098866 1102 74-75 0.3059099211668368 1102 76-77 0.37692487963268206 1102 78-79 0.2542564684118851 1102 80-81 0.26265957476464763 1102 82-83 0.2665362121910704 1102 84-85 0.2715830128230543 1102 86-87 0.3378369924363298 1102 88-89 0.3726857706843276 1102 90-91 0.34302207275424834 1102 92-93 0.29329492832507853 1102 94-95 0.30514017206022714 1102 96-97 0.317765002664931 1102 98-99 0.3521485499999386 1102 100-101 0.24917427010342408 1102 102-103 0.09305785034280945 1102 104-105 0.2025146609624251 1102 106-107 0.3589969394803738 1102 108-109 0.34314778848314376 1102 110-111 0.39562715428339956 1102 112-113 0.3478826113687532 1102 114-115 0.43995176503590727 1102 116-117 0.34431325901213583 1102 118-119 0.43214968540152654 1102 120-121 0.5110567194595461 1102 122-123 0.4714764834329408 1102 124-125 0.49115991686461413 1103 1 0.2500660868186273 1103 2 0.5500541334513009 1103 3 0.4017952183206006 1103 4 0.4797338706146874 1103 5 0.2155401621068762 1103 6 0.18275088342058865 1103 7 0.21317370410132241 1103 8 0.18113602701334486 1103 9 0.3425819665393348 1103 10-11 0.2627947802623538 1103 12-13 0.16156960885436433 1103 14-15 0.33992308336926413 1103 16-17 0.39737962764521484 1103 18-19 0.2503190928800265 1103 20-21 0.12778950684680268 1103 22-23 0.19426887063843878 1103 24-25 0.06251994983617948 1103 26-27 0.10510121945522144 1103 28-29 0.2431679419439945 1103 30-31 0.2479551425425015 1103 32-33 0.18165629096660751 1103 34-35 0.1599367973193182 1103 36-37 0.22226490150777067 1103 38-39 0.2534871814694526 1103 40-41 0.3353199432585612 1103 42-43 0.2608821851081444 1103 44-45 0.29390364300773086 1103 46-47 0.2910816850575415 1103 48-49 0.4069685135841681 1103 50-51 0.34958413841755487 1103 52-53 0.37715123729341826 1103 54-55 0.44579854726706003 1103 56-57 0.46464815755611966 1103 58-59 0.39806412815379844 1103 60-61 0.3858316977526499 1103 62-63 0.4313172047593312 1103 64-65 0.3582475334446258 1103 66-67 0.24011078467780322 1103 68-69 0.2983718237561348 1103 70-71 0.2901321563667594 1103 72-73 0.3105430224779866 1103 74-75 0.33421978694531873 1103 76-77 0.31226390721207054 1103 78-79 0.2324757917771194 1103 80-81 0.1369432917170883 1103 82-83 0.18395779784223265 1103 84-85 0.1275255041209178 1103 86-87 0.11898988281126321 1103 88-89 0.1650947469298245 1103 90-91 0.1497342134549946 1103 92-93 0.09957353282914383 1103 94-95 0.1740509958304557 1103 96-97 0.08769866313082275 1103 98-99 0.1687677646161525 1103 100-101 0.20934518124860446 1103 102-103 -0.025322720247306307 1103 104-105 0.20548138637127167 1103 106-107 0.32591189327221315 1103 108-109 0.33129141712247545 1103 110-111 0.38085533277203254 1103 112-113 0.5228818365559285 1103 114-115 0.4496918503335223 1103 116-117 0.48023888272449256 1103 118-119 0.6060394623834675 1103 120-121 0.6625643975255961 1103 122-123 0.6036037293580527 1103 124-125 0.6107460244167697 1104 1 0.18751901042055863 1104 2 0.4782087100979844 1104 3 -0.006286434219774151 1104 4 0.354281782551908 1104 5 0.1309280240449695 1104 6 0.12693594011687281 1104 7 0.08385213239316869 1104 8 0.05888489610919834 1104 9 0.20605553500048757 1104 10-11 0.15927494072774095 1104 12-13 0.21351673388119252 1104 14-15 0.26409296855876363 1104 16-17 0.22845065703801737 1104 18-19 0.22086609980843974 1104 20-21 0.1070658994874023 1104 22-23 0.07398602683620226 1104 24-25 0.09388930619981295 1104 26-27 0.1113404034817691 1104 28-29 0.18240453465720208 1104 30-31 0.1908038005172088 1104 32-33 0.1816686027978136 1104 34-35 0.2292672168922536 1104 36-37 0.23608829251201513 1104 38-39 0.13517518229927816 1104 40-41 0.16177332221982255 1104 42-43 0.25283982336821964 1104 44-45 0.26709483872801343 1104 46-47 0.19589112645965656 1104 48-49 0.21435746800398192 1104 50-51 0.17473977899678061 1104 52-53 0.29852496881793655 1104 54-55 0.3544158536810613 1104 56-57 0.32971506730314104 1104 58-59 0.39202960601700454 1104 60-61 0.4221010439813213 1104 62-63 0.46737724985750617 1104 64-65 0.294768029134417 1104 66-67 0.3651738902474122 1104 68-69 0.3464601116003223 1104 70-71 0.4110187557176417 1104 72-73 0.4124615507490432 1104 74-75 0.3615766758847556 1104 76-77 0.25910950899772445 1104 78-79 0.3158638245344392 1104 80-81 0.2515983796318899 1104 82-83 0.21720878266977195 1104 84-85 0.22845474404198995 1104 86-87 0.2029451519657357 1104 88-89 0.19912708695809656 1104 90-91 0.2263253421798872 1104 92-93 0.24152918454254646 1104 94-95 0.1855651750155367 1104 96-97 0.2504312139947409 1104 98-99 0.2703325805191845 1104 100-101 0.33513813455119745 1104 102-103 0.1991625262533958 1104 104-105 0.1002128504633113 1104 106-107 0.23865277097849358 1104 108-109 0.1977247022235673 1104 110-111 0.179549993172472 1104 112-113 0.1568913610361271 1104 114-115 0.20446027608925732 1104 116-117 0.18087706381856705 1104 118-119 0.33221029968791527 1104 120-121 0.3992291379160626 1104 122-123 0.35545181254273217 1104 124-125 0.45627179789715555 1105 1 0.18309248386900379 1105 2 0.5256862673112828 1105 3 0.1551723397856648 1105 4 0.3921726121673572 1105 5 0.16674094108913096 1105 6 0.1112682939499976 1105 7 0.13723521077442058 1105 8 0.10106725807049344 1105 9 0.15161579422738924 1105 10-11 0.1537916329514104 1105 12-13 0.1825647965717394 1105 14-15 0.20510656114435477 1105 16-17 0.22888309006140872 1105 18-19 0.16341750930955357 1105 20-21 0.11032905167815699 1105 22-23 0.2155294716791829 1105 24-25 0.17484994207956106 1105 26-27 0.2316131107905619 1105 28-29 0.2087244832833477 1105 30-31 0.18287538642344003 1105 32-33 0.14448328427327795 1105 34-35 0.16021542284777723 1105 36-37 0.2808063114815553 1105 38-39 0.23913890213115963 1105 40-41 0.1823870324975374 1105 42-43 0.23484554731267338 1105 44-45 0.36050975958053044 1105 46-47 0.3074158289732978 1105 48-49 0.43238879616634307 1105 50-51 0.44164982342128667 1105 52-53 0.3935550668218326 1105 54-55 0.4738082466625073 1105 56-57 0.4546585028296235 1105 58-59 0.5322031448710973 1105 60-61 0.4514798709957617 1105 62-63 0.41308284175481447 1105 64-65 0.4186647842349558 1105 66-67 0.49304453727844333 1105 68-69 0.4348743051953221 1105 70-71 0.41043647437260944 1105 72-73 0.3896950631556777 1105 74-75 0.3248283060348527 1105 76-77 0.200100879825861 1105 78-79 0.3559762357305978 1105 80-81 0.3033112612163009 1105 82-83 0.4531591656445286 1105 84-85 0.503852073818571 1105 86-87 0.48162738035882313 1105 88-89 0.5198879361667181 1105 90-91 0.47721397481216954 1105 92-93 0.512810735873142 1105 94-95 0.5225490954944441 1105 96-97 0.48319497318409077 1105 98-99 0.40318624870152675 1105 100-101 0.36301265553566964 1105 102-103 0.13751770741657054 1105 104-105 0.2791151099050637 1105 106-107 0.30371451181433784 1105 108-109 0.4252139430903057 1105 110-111 0.4251526995210959 1105 112-113 0.43960618482590874 1105 114-115 0.5151130776468236 1105 116-117 0.46066669626356926 1105 118-119 0.45087591048440956 1105 120-121 0.46572721234729997 1105 122-123 0.44161186433487387 1105 124-125 0.40704264333164275 1106 1 0.17781378300659867 1106 2 0.4314224858799527 1106 3 0.3572943184508972 1106 4 0.4040068550521312 1106 5 0.2002637592564085 1106 6 0.13039773323971104 1106 7 0.1225639218170187 1106 8 0.05149246276501884 1106 9 0.23274190495876468 1106 10-11 0.21800613135893343 1106 12-13 0.15156441572358403 1106 14-15 0.25584537475235436 1106 16-17 0.25134972267292 1106 18-19 0.14850250934591713 1106 20-21 0.10617051249897003 1106 22-23 0.04774006798377428 1106 24-25 0.04424561149972561 1106 26-27 0.11144790540524241 1106 28-29 0.19242186442963316 1106 30-31 0.1409088056218195 1106 32-33 0.23824998614643533 1106 34-35 0.265410750686236 1106 36-37 0.22113835835405382 1106 38-39 0.1720310030313854 1106 40-41 0.24545466894843315 1106 42-43 0.27827223540622015 1106 44-45 0.26665488999602616 1106 46-47 0.2522369303243579 1106 48-49 0.32011994100134444 1106 50-51 0.3461107914845769 1106 52-53 0.29878204868654734 1106 54-55 0.36011507393377684 1106 56-57 0.49135172282351647 1106 58-59 0.4812550465668295 1106 60-61 0.36176888384461137 1106 62-63 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66-67 -1.2966341847612028 2216 68-69 -1.3261427027077985 2216 70-71 -1.2255177958062333 2216 72-73 -1.0887360315659222 2216 74-75 -1.14455477233399 2216 76-77 -1.141756371682078 2216 78-79 -1.1067222850780603 2216 80-81 -1.123444928838257 2216 82-83 -1.1409475221360665 2216 84-85 -1.265014751121484 2216 86-87 -1.2772422540961301 2216 88-89 -1.4403750636167914 2216 90-91 -1.4238723506747917 2216 92-93 -1.4071195086965496 2216 94-95 -1.5251144462457056 2216 96-97 -1.5255111783156536 2216 98-99 -1.6851360006293419 2216 100-101 -1.8065867716962742 2216 102-103 -1.8834168272377596 2216 104-105 -1.9102193350718224 2216 106-107 -2.1387324945725474 2216 108-109 -2.209290568755467 2216 110-111 -2.0437464165966013 2216 112-113 -2.21494794556639 2216 114-115 -2.2672706355994094 2216 116-117 -2.291847880375375 2216 118-119 -2.4275054400125065 2216 120-121 -2.515033083734213 2216 122-123 -2.4762401297720373 2216 124-125 -2.6647784696254497 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43.0 3 17.0 4 49.0 5 195.0 6 595.0 7 1161.0 8 1863.0 9 3629.0 10 7486.0 11 9816.0 12 9921.0 13 11890.0 14 15715.0 15 16261.0 16 11085.0 17 10883.0 18 11276.0 19 11393.0 20 11913.0 21 12181.0 22 12655.0 23 13459.0 24 15014.0 25 17190.0 26 20073.0 27 24783.0 28 30346.0 29 37183.0 30 45790.0 31 57483.0 32 73874.0 33 97930.0 34 137813.0 35 216365.0 36 337399.0 37 466316.0 38 184841.0 39 1089.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.20624679751836 17.818232959178346 10.744619960114724 24.230900283188568 2 11.666824129013806 23.51382648475377 35.29546905968133 29.523880326551094 3 23.283785053532174 29.107170698917795 27.871268778078708 19.737775469471327 4 14.016236657674982 19.59121826559455 32.31399475987042 34.07855031686005 5 11.173505078795545 39.26736276926651 35.230346287381096 14.328785864556847 6 33.2084092704359 28.147100278278288 19.25406962636364 19.39042082492217 7 22.549232695310987 32.782199047483836 23.939957924082655 20.728610333122525 8 28.534544844409453 32.34068586326618 23.091986215619716 16.032783076704654 9 29.485718710876498 14.318615366744536 21.09139250635656 35.10427341602241 10-11 23.52976252397747 27.599434065858897 25.555395798963797 23.315407611199838 12-13 28.07046710023437 25.4074668196278 25.36474337147638 21.15732270866145 14-15 29.277163824607445 22.08439933679118 25.170080846321774 23.4683559922796 16-17 22.614869813050266 26.562902933946386 32.6018881605729 18.22033909243045 18-19 25.39467468604396 24.93207191626118 31.947727771396124 17.725525626298737 20-21 20.576096100685167 25.653359876528707 32.95334952530204 20.817194497484085 22-23 19.23540572284103 27.589901779837117 26.5645400689219 26.610152428399953 24-25 21.188399437266874 29.17404716116625 24.90992397940087 24.727629422166007 26-27 23.939803043405313 23.591140825255877 26.684701661095268 25.78435447024355 28-29 22.724714837534577 22.827891661495347 31.0180457478345 23.429347753135573 30-31 25.66778094709534 25.351643671188324 28.865189277094437 20.115386104621898 32-33 18.8492107538817 25.61894190683927 31.363440416112752 24.168406923166277 34-35 16.69342664736506 27.47414521221783 33.276810403041445 22.55561773737566 36-37 24.352689115760402 25.2077853353812 28.57269711792873 21.866828430929672 38-39 26.048503465455152 23.406961886446652 28.4366344428813 22.1079002052169 40-41 21.660761833221688 26.36242899679784 28.622605638438337 23.354203531542137 42-43 25.496689425521758 25.2161230785116 27.69733734302198 21.58985015294467 44-45 27.93835749041676 21.756656642445048 27.768040372229898 22.5369454949083 46-47 22.60875850230385 24.760593192994232 34.28402534880419 18.346622955897725 48-49 25.39599117180139 24.620761754798075 31.63781153602912 18.345435537371415 50-51 20.311232721124433 25.878728429638997 32.05289175131326 21.75714709792331 52-53 19.07409749738639 27.82926986667355 25.877205436311773 27.21942719962829 54-55 21.075439796590043 28.96400314924044 24.31957046425483 25.64098658991469 56-57 23.87379806141019 23.717471831076807 25.85136617664141 26.557363930871592 58-59 22.947637424334335 22.84998515726842 29.887350120677862 24.315027297719382 60-61 25.487783786574425 25.357348442803858 28.012700215542274 21.142167555079443 62-63 19.100711160443478 25.79042063010622 30.21987893493721 24.888989274513094 64-65 17.00393655054918 27.31475625653403 32.23353424798977 23.44777294492701 66-67 24.122020160301503 25.442016546419026 27.687631487241703 22.748331806037765 68-69 25.803353166664518 23.685334090527757 27.59689722376386 22.91441551904387 70-71 21.660862943507272 26.289885001097073 27.824778327030554 24.224473728365105 72-73 25.235405722841026 25.31550484647453 27.02931116818751 22.419778262496934 74-75 27.507918274629255 22.085623201125465 27.188580131390445 23.21787839285484 76-77 22.410407981517572 24.836717045909214 33.51504278578712 19.237832186786097 78-79 24.85540598097549 24.698847429626092 31.213825681797648 19.23192090760077 80-81 19.997960737748294 26.032447501903743 31.569431716981377 22.40016004336659 82-83 18.904838730494713 27.613133881438838 25.809470953419638 27.672556434646804 84-85 20.73619948631242 28.604783231585333 24.448818389499092 26.21019889260316 86-87 23.249757998941647 23.674544070006064 25.94914751093845 27.126550420113837 88-89 22.45369713083545 22.823603815227354 29.683217387937376 25.03948166599982 90-91 24.75997367028485 25.293331096167993 27.763781153602913 22.182914079944243 92-93 18.865628105680248 25.71057964093496 29.656061642509584 25.767730610875205 94-95 16.963566160014416 27.064585126303392 31.637801031821468 24.334047681860728 96-97 23.58407546813284 25.235560413201657 27.4566858216135 23.723678297052 98-99 24.945376350747846 23.778066901063045 27.338800861174356 23.93775588701475 100-101 21.28336710592548 25.968481782160328 27.603789413905705 25.144361698008495 102-103 24.43558092480625 24.974095243798995 27.048644149965178 23.54167968142958 104-105 26.49030509678895 22.071867610828217 27.177331860598297 24.260495431784534 106-107 21.796147799777295 24.724166962928194 32.85730108458828 20.622384152706232 108-109 24.06329190726447 24.939319652015858 30.354477539686535 20.642910901033133 110-111 19.46154148022005 26.196649072784144 30.978081829626085 23.36372761736972 112-113 18.4794930903535 27.53276037582942 25.625937011163195 28.361809522653886 114-115 20.06857821069129 28.51573727386893 24.43998722228549 26.975697293154287 116-117 22.37530417473775 24.0319670444119 25.730361538936304 27.862367241914043 118-119 21.49157844565429 23.302740639260445 29.053239343378507 26.15244157170676 120-121 23.654577871499775 25.44615910490903 27.340156978608427 23.559106044982773 122-123 18.47690870430446 26.10248439588943 28.760068552505935 26.66053834730017 124-125 16.79558147963435 27.485779100828218 30.334966839413546 25.383672580123886 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2178.0 1 1636.5 2 3256.0 3 4361.5 4 2138.5 5 1141.5 6 2096.5 7 4439.5 8 6502.5 9 6540.0 10 4663.5 11 2598.0 12 1968.0 13 1946.0 14 2194.5 15 2682.0 16 2845.0 17 3162.5 18 3421.0 19 3133.0 20 3068.5 21 3536.0 22 4098.0 23 4546.0 24 5057.5 25 5768.0 26 6877.0 27 8181.0 28 9230.5 29 10527.0 30 12685.0 31 14665.0 32 16742.5 33 19012.5 34 20900.5 35 22704.0 36 25027.5 37 27248.5 38 28133.5 39 28366.5 40 28538.5 41 29568.5 42 30503.5 43 31700.5 44 33871.0 45 36231.5 46 41857.5 47 57656.5 48 76598.5 49 139433.5 50 365846.0 51 362056.5 52 118365.5 53 41001.0 54 29983.5 55 26034.5 56 22829.5 57 20062.0 58 18473.0 59 16851.5 60 14777.5 61 12414.5 62 10267.0 63 8130.5 64 6316.5 65 4945.0 66 3466.0 67 2612.5 68 2007.5 69 1351.0 70 1061.0 71 891.0 72 777.0 73 747.0 74 573.5 75 413.5 76 275.0 77 136.5 78 77.5 79 40.0 80 17.5 81 8.5 82 6.0 83 3.5 84 1.0 85 1.0 86 1.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15116354108855304 2 2.0650756979310526E-4 3 4.646420320344868E-4 4 0.0 5 0.0 6 0.00335574800913796 7 0.0 8 0.0 9 0.0 10-11 2.5813446224138157E-5 12-13 0.005136875798603493 14-15 0.0014713664347758748 16-17 5.420823707069012E-4 18-19 0.0 20-21 3.35574800913796E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 4.646420320344868E-4 30-31 0.0 32-33 0.0 34-35 1.5488067734482892E-4 36-37 0.0 38-39 0.0 40-41 1.2906723112069077E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 6.195227093793157E-4 96-97 0.00265878496108623 98-99 0.004388285858103486 100-101 0.0 102-103 0.0037171362562758944 104-105 0.022819086462138125 106-107 0.01763058377108636 108-109 0.02026355528594845 110-111 0.01636572490610359 112-113 0.015410627395810478 114-115 0.040862685372810695 116-117 0.04096593915770725 118-119 0.023541862956413997 120-121 0.014352276100620813 122-123 0.003381561455362098 124-125 0.0019101950205862234 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1936975.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.038866094462485 #Duplication Level Percentage of deduplicated Percentage of total 1 86.34232576866961 43.20472077422916 2 9.646233676309006 9.65373190489442 3 2.1274564957614026 3.1936653213959767 4 0.7139069573913115 1.428923785792359 5 0.3408023858178919 0.8526682474307417 6 0.19245768334888705 0.5778218547567253 7 0.12645973473393082 0.44295312128846875 8 0.07747496695351286 0.31014075976477895 9 0.05938973735243729 0.26746156032875157 >10 0.31927038448653755 2.8787070633792187 >50 0.02622944863754301 0.8883207851768872 >100 0.01900323313733123 2.156889142020953 >500 0.004210284985071002 1.562375818938985 >1k 0.002275829721660001 2.227206901045898 >5k 0.0010241233747470004 4.144090969145352 >10k+ 0.0014792893190790006 26.210321990411416 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 111610 5.762077466152118 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 101265 5.2279972637747 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 59658 3.079957149679268 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 31220 1.6117915822351865 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 30683 1.584067940990462 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 25971 1.340802023774184 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 19176 0.9899972895881465 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 15272 0.7884459014700758 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 14820 0.7651105460834549 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 13368 0.6901482982485577 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 12953 0.668723137882523 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 12349 0.6375404948437641 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 11970 0.6179739026058675 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 9697 0.5006259760709354 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9640 0.4976832432013836 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 9537 0.4923656732792111 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 9191 0.47450276849210754 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8315 0.4292776107074175 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7393 0.38167761587010673 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 7251 0.3743465971424515 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6575 0.3394468178474167 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 5181 0.26747892977451954 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 4063 0.20976006401734665 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3408 0.17594444946372564 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3196 0.16499954826469107 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 2973 0.15348675124872546 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 2939 0.15173143690548405 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2759 0.14243859626479433 No Hit TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA 1960 0.10118870919862157 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 5.162689244827631E-5 0.0 0.0 2.839479084655197E-4 0.0 12-13 5.162689244827631E-5 0.0 0.0 9.292840640689735E-4 0.0 14-15 1.0325378489655261E-4 0.0 0.0 0.0022457698215000194 0.0 16-17 1.5488067734482892E-4 0.0 0.0 0.004285032073206933 0.0 18-19 1.8069412356896709E-4 0.0 0.0 0.00629848087868971 0.0 20-21 2.0650756979310523E-4 0.0 0.0 0.009292840640689736 0.0 22-23 2.5813446224138154E-4 0.0 0.0 0.013913447514810464 0.0 24-25 2.5813446224138154E-4 0.0 0.0 0.01861149472760361 0.0 26-27 2.5813446224138154E-4 0.0 0.0 0.023257915047948476 0.0 28-29 3.0976135468965783E-4 0.0 0.0 0.03154403128589682 0.0 30-31 3.6138824713793417E-4 0.0 0.0 0.04243730559248313 0.0 32-33 3.6138824713793417E-4 0.0 0.0 0.059345112869293615 0.0 34-35 3.6138824713793417E-4 0.0 0.0 0.08748176925360421 0.0 36-37 3.6138824713793417E-4 0.0 0.0 0.12545334864931143 0.0 38-39 4.1301513958621046E-4 0.0 0.0 0.18306896062158778 0.0 40-41 4.1301513958621046E-4 0.0 0.0 0.28629693207191625 0.0 42-43 4.90455478258625E-4 0.0 0.0 0.35648369235534794 0.0 44-45 5.678958169310394E-4 0.0 0.0 0.42669626608500366 0.0 46-47 5.937092631551775E-4 0.0 0.0 0.5227480994850218 0.0 48-49 6.195227093793157E-4 0.0 0.0 0.6009370280979363 0.0 50-51 6.195227093793157E-4 0.0 0.0 0.6873346326101266 0.0 52-53 6.195227093793157E-4 0.0 0.0 0.7999328850398173 0.0 54-55 6.195227093793157E-4 0.0 0.0 0.9174615056983182 0.0 56-57 6.195227093793157E-4 0.0 0.0 1.0425018392080436 0.0 58-59 6.195227093793157E-4 0.0 0.0 1.2016417351798552 0.0 60-61 6.195227093793157E-4 0.0 0.0 1.3409310910053045 0.0 62-63 6.195227093793157E-4 0.0 0.0 1.4762193626660127 0.0 64-65 6.195227093793157E-4 0.0 0.0 1.63938615624879 0.0 66-67 6.195227093793157E-4 0.0 0.0 1.8173698679642225 0.0 68-69 6.195227093793157E-4 0.0 0.0 2.0507750487228797 0.0 70-71 6.453361556034538E-4 0.0 0.0 2.4205784793298832 0.0 72-73 7.227764942758683E-4 0.0 0.0 2.6962660850036784 0.0 74-75 7.744033867241446E-4 0.0 0.0 2.942087533396146 0.0 76-77 7.744033867241446E-4 0.0 0.0 3.234140863976045 0.0 78-79 7.744033867241446E-4 0.0 0.0 3.50670504265672 0.0 80-81 8.518437253965591E-4 0.0 0.0 3.808722363479136 0.0 82-83 8.776571716206973E-4 0.0 0.0 4.201086746086037 0.0 84-85 9.034706178448354E-4 0.0 0.0 4.568412085855522 0.0 86-87 9.292840640689735E-4 0.0 0.0 4.928690354805818 0.0 88-89 9.292840640689735E-4 0.0 0.0 5.363311348881632 0.0 90-91 9.292840640689735E-4 0.0 0.0 5.751803714554912 0.0 92-93 9.292840640689735E-4 0.0 0.0 6.15170562345926 0.0 94-95 9.292840640689735E-4 0.0 0.0 6.607493643438867 0.0 96-97 9.292840640689735E-4 0.0 0.0 7.055950644690819 0.0 98-99 9.292840640689735E-4 0.0 0.0 7.588404599956117 0.0 100-101 9.292840640689735E-4 0.0 0.0 8.435730972263453 0.0 102-103 9.292840640689735E-4 0.0 0.0 9.079647388324577 0.0 104-105 9.8091095651725E-4 0.0 0.0 9.655932575278463 0.0 106-107 0.0010325378489655262 0.0 0.0 10.270421662644072 0.0 108-109 0.0010325378489655262 0.0 0.0 10.810981040023748 0.0 110-111 0.0011099781876379405 0.0 0.0 11.379677073787736 0.0 112-113 0.0011357916338620789 0.0 0.0 12.118225583706554 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 2595 0.0 54.108864 5 AAAGTAC 2575 0.0 51.98752 3 AAGTACT 2800 0.0 49.722347 4 AAAAAGT 2740 0.0 48.926346 1 AAAAGTA 2865 0.0 46.51759 2 GTTATCA 940 0.0 39.932228 1 TTATCAA 1160 0.0 30.77422 2 CGCATGT 125 0.0016209756 23.832567 1 CGATGTA 155 2.1077515E-4 23.031029 2 TATAACG 185 2.6928248E-5 22.512314 2 CCTAGAC 305 7.012204E-9 21.457872 3 CGGGAGT 560 0.0 21.248865 4 CGTAGCT 170 3.9363903E-4 20.99888 4 ATAACGC 200 4.91337E-5 20.823889 3 GACATGG 1345 0.0 20.790712 2 GGACATG 1330 0.0 20.607107 1 GTCGGGA 505 0.0 20.028633 2 CCGATGT 225 1.2014818E-4 18.536442 1 GTATATA 650 0.0 18.332745 1 TCGGGAG 665 0.0 17.893782 3 >>END_MODULE