##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364154_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1475494 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.632981225270996 31.0 31.0 34.0 27.0 34.0 2 30.72658241917622 33.0 31.0 34.0 27.0 34.0 3 30.875994073849164 33.0 31.0 34.0 27.0 34.0 4 34.169365649741714 37.0 35.0 37.0 30.0 37.0 5 34.23438387414656 37.0 35.0 37.0 31.0 37.0 6 34.16756218595264 37.0 35.0 37.0 30.0 37.0 7 34.108623281423036 37.0 35.0 37.0 30.0 37.0 8 33.99736630579318 37.0 35.0 37.0 30.0 37.0 9 35.40850183057336 39.0 37.0 39.0 28.0 39.0 10-11 35.36442540599962 39.0 37.0 39.0 28.0 39.0 12-13 35.2452121120113 39.0 37.0 39.0 27.0 39.0 14-15 36.456114697857124 40.0 37.0 41.0 27.0 41.0 16-17 36.49327072831201 40.0 37.0 41.0 27.0 41.0 18-19 36.44196689379963 40.0 37.0 41.0 27.0 41.0 20-21 36.48155837976976 40.0 37.0 41.0 27.0 41.0 22-23 36.50851240330357 40.0 37.0 41.0 27.5 41.0 24-25 36.54235733930467 40.0 37.0 41.0 28.5 41.0 26-27 36.40216192000781 40.0 37.0 41.0 28.0 41.0 28-29 36.30485383200474 40.0 36.5 41.0 28.0 41.0 30-31 36.3411006754348 40.0 36.0 41.0 29.0 41.0 32-33 36.01896551256732 40.0 36.0 41.0 28.0 41.0 34-35 36.087371415946116 40.0 36.0 41.0 29.0 41.0 36-37 36.11042572860343 40.0 36.0 41.0 29.0 41.0 38-39 35.960992047409206 40.0 35.5 41.0 28.5 41.0 40-41 35.62939598534457 40.0 35.0 41.0 27.0 41.0 42-43 35.382928700489465 39.0 35.0 41.0 26.5 41.0 44-45 35.126058459065234 39.0 34.5 41.0 25.5 41.0 46-47 34.87114654481821 39.0 34.0 40.5 24.5 41.0 48-49 34.58896850817421 38.0 33.5 40.0 24.0 41.0 50-51 34.47959530841874 38.0 33.0 40.0 23.5 41.0 52-53 34.33069297469187 38.0 33.0 40.0 23.0 41.0 54-55 34.03366974043948 38.0 33.0 40.0 22.5 41.0 56-57 33.60978560400788 38.0 32.5 40.0 20.0 41.0 58-59 33.20619602655111 38.0 32.0 40.0 18.0 41.0 60-61 32.92128873448486 37.0 31.5 40.0 15.5 41.0 62-63 32.339103717127955 36.0 30.5 40.0 12.0 41.0 64-65 32.11368565375393 36.0 31.0 39.0 8.0 41.0 66-67 31.687282022156648 35.0 30.5 39.0 8.0 41.0 68-69 31.242778011974295 35.0 30.0 38.5 4.5 40.0 70-71 30.590397859970963 35.0 29.0 37.5 2.0 40.0 72-73 30.344522919103703 35.0 29.0 37.0 2.0 39.5 74-75 29.98246383922944 35.0 29.0 37.0 2.0 39.0 76-77 29.357314906058583 34.0 28.5 36.0 2.0 39.0 78-79 28.98901520439934 34.0 28.5 36.0 2.0 37.5 80-81 28.7717862627703 34.0 28.0 35.0 2.0 37.0 82-83 28.558421111844574 34.0 28.0 35.0 2.0 37.0 84-85 28.253331765496846 34.0 27.5 35.0 2.0 36.0 86-87 27.84178654742073 34.0 26.5 35.0 2.0 36.0 88-89 27.468252327694998 34.0 26.0 35.0 2.0 36.0 90-91 27.320982667499834 34.0 26.0 35.0 2.0 35.0 92-93 26.937418925458186 34.0 25.0 35.0 2.0 35.0 94-95 26.860880830420186 34.0 25.0 35.0 2.0 35.0 96-97 26.758219281135673 34.0 25.0 35.0 2.0 35.0 98-99 26.502875308201865 33.5 24.5 35.0 2.0 35.0 100-101 25.924545270939767 33.0 20.0 35.0 2.0 35.0 102-103 24.651110746638075 31.0 18.5 34.5 2.0 35.0 104-105 25.4096129838549 32.5 19.0 35.0 2.0 35.0 106-107 25.360990285287503 33.0 18.5 35.0 2.0 35.0 108-109 25.309504816691902 33.0 18.5 35.0 2.0 35.0 110-111 25.36223969734882 33.0 19.0 35.0 2.0 35.0 112-113 25.2592558831144 33.0 18.5 35.0 2.0 35.0 114-115 25.049494948810363 33.0 16.5 35.0 2.0 35.0 116-117 24.65046248917312 33.0 8.5 35.0 2.0 35.0 118-119 24.36641490917618 33.0 2.0 35.0 2.0 35.0 120-121 24.181981085656737 33.0 2.0 35.0 2.0 35.0 122-123 23.65496267690685 32.5 2.0 35.0 2.0 35.0 124-125 22.604483312029735 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16147168407993462 1101 2 0.29261389088512146 1101 3 0.2210629678563727 1101 4 0.3538687756256209 1101 5 0.14721864317100142 1101 6 0.11718918630850794 1101 7 0.13962132706447505 1101 8 0.18696167696660382 1101 9 0.3239787943357513 1101 10-11 0.348518669013572 1101 12-13 0.3505022602710497 1101 14-15 0.2803477306478541 1101 16-17 0.3663742943526671 1101 18-19 0.3773839483429029 1101 20-21 0.3042286863645316 1101 22-23 0.3465719629009527 1101 24-25 0.36869410413878967 1101 26-27 0.402644776747195 1101 28-29 0.49017703854376293 1101 30-31 0.433072885496415 1101 32-33 0.4684210681924057 1101 34-35 0.3985122086100503 1101 36-37 0.40247254899665563 1101 38-39 0.39218074419289195 1101 40-41 0.481349741914876 1101 42-43 0.6304806801116527 1101 44-45 0.4717288504682955 1101 46-47 0.48117555292670033 1101 48-49 0.48440911143033105 1101 50-51 0.46378168251535357 1101 52-53 0.4688662510052666 1101 54-55 0.5279801827309285 1101 56-57 0.34548303058548413 1101 58-59 0.3346425587736235 1101 60-61 0.4269951502615328 1101 62-63 0.44021657682549886 1101 64-65 0.5345301264772573 1101 66-67 0.42906918868444777 1101 68-69 0.4531156630413946 1101 70-71 0.4711647821305114 1101 72-73 0.45907947577499897 1101 74-75 0.3689744719389907 1101 76-77 0.29320156329573166 1101 78-79 0.26584582508867527 1101 80-81 0.25954023704017715 1101 82-83 0.2293969288590283 1101 84-85 0.21262747709197782 1101 86-87 0.163672028076018 1101 88-89 0.13973004584119053 1101 90-91 0.07470700454900836 1101 92-93 0.2216076761864727 1101 94-95 0.22706701578541555 1101 96-97 0.039164691705117605 1101 98-99 0.10753135330804753 1101 100-101 0.12254795952756581 1101 102-103 0.17611092070970358 1101 104-105 0.1531153762621642 1101 106-107 0.12720641496152396 1101 108-109 0.11686086083603442 1101 110-111 0.025323457992200815 1101 112-113 0.17836686011958847 1101 114-115 0.1160449699511652 1101 116-117 0.016543667158078534 1101 118-119 0.12531034712871048 1101 120-121 0.15013733159590004 1101 122-123 0.12149574883832415 1101 124-125 0.0729531413859128 1102 1 0.19189314502849086 1102 2 0.33973702791474736 1102 3 0.34489069729598043 1102 4 0.39133441969171656 1102 5 0.32253977909947906 1102 6 0.3416071234208502 1102 7 0.327830506448457 1102 8 0.39124096724821555 1102 9 0.48036362634014296 1102 10-11 0.46549265822462615 1102 12-13 0.4768847158714138 1102 14-15 0.5122731945700352 1102 16-17 0.4502823844473909 1102 18-19 0.5465703256343204 1102 20-21 0.46001556075412964 1102 22-23 0.5547232858454407 1102 24-25 0.5198277994544114 1102 26-27 0.6541377424810619 1102 28-29 0.6185514312660203 1102 30-31 0.3220889403645302 1102 32-33 0.4919497637302399 1102 34-35 0.45306601579662953 1102 36-37 0.43317572584849273 1102 38-39 0.439130242117848 1102 40-41 0.3545433351002174 1102 42-43 0.486559389404718 1102 44-45 0.3922792726564879 1102 46-47 0.3575531418572808 1102 48-49 0.4415871004195324 1102 50-51 0.46179121457679173 1102 52-53 0.504739943110728 1102 54-55 0.4893074209123611 1102 56-57 0.6266884844878362 1102 58-59 0.6007736407794013 1102 60-61 0.3822813820670703 1102 62-63 0.3683851736929782 1102 64-65 0.36870322051490234 1102 66-67 0.4353163398461426 1102 68-69 0.4330926225738452 1102 70-71 0.32464891366502613 1102 72-73 0.4320599088241295 1102 74-75 0.2621848615627371 1102 76-77 0.2675845154800598 1102 78-79 0.27060203064358035 1102 80-81 0.34259730269173616 1102 82-83 0.3733201478690873 1102 84-85 0.29134990098001623 1102 86-87 0.4173358958260138 1102 88-89 0.33910419210794274 1102 90-91 0.26682393611555 1102 92-93 0.28284342097610704 1102 94-95 0.26337659621971454 1102 96-97 0.29561771218075705 1102 98-99 0.3443967625251929 1102 100-101 0.17400610561654517 1102 102-103 -0.014107917820069815 1102 104-105 0.1292227823785943 1102 106-107 0.23787283284648097 1102 108-109 0.208736056650622 1102 110-111 0.13485070785363718 1102 112-113 0.24407865556813135 1102 114-115 0.27564465055070997 1102 116-117 0.27557326789648684 1102 118-119 0.1701035430468778 1102 120-121 0.16388282717163705 1102 122-123 -0.008071528922904747 1102 124-125 -0.17020542279590956 1103 1 -0.18553335535420246 1103 2 -0.09227503817863081 1103 3 -0.10504582064558221 1103 4 -0.06950642493482206 1103 5 -0.17902989358523058 1103 6 0.013268732533454397 1103 7 -0.025453722086957953 1103 8 0.032380582754043985 1103 9 0.06226538672914472 1103 10-11 0.013048193770075045 1103 12-13 -0.06134895213093472 1103 14-15 -0.05333087548533655 1103 16-17 -0.08306910805632128 1103 18-19 -0.20239820347288173 1103 20-21 -0.10592023682364982 1103 22-23 -0.1227024941734669 1103 24-25 -0.14924149053703673 1103 26-27 -0.032205961089239565 1103 28-29 -0.0037219467898950143 1103 30-31 -0.16571548086818666 1103 32-33 -0.026691720219155002 1103 34-35 -0.16515315488455684 1103 36-37 -0.11733726321097748 1103 38-39 -0.12485670128857151 1103 40-41 -0.0937742552829306 1103 42-43 -0.05536479230704572 1103 44-45 -0.09765796564884965 1103 46-47 -0.10995160804141335 1103 48-49 -0.13479190097617533 1103 50-51 -0.08113391261244374 1103 52-53 0.006603974446548477 1103 54-55 0.2266852967396531 1103 56-57 0.13765071041193266 1103 58-59 0.11594621292832841 1103 60-61 0.17289567228363012 1103 62-63 0.15435347768649166 1103 64-65 -0.02824657913392059 1103 66-67 0.05011567350964441 1103 68-69 0.3399723668446981 1103 70-71 0.20283782906730607 1103 72-73 0.06893208927525762 1103 74-75 0.0027986115064564387 1103 76-77 -0.06219316224719407 1103 78-79 -0.10998947806831794 1103 80-81 -0.04426695375777001 1103 82-83 -0.018291756142438942 1103 84-85 0.0940515353053506 1103 86-87 -0.0010043860163193585 1103 88-89 -0.03431483450049555 1103 90-91 -0.06106881972614886 1103 92-93 -0.08649609547970272 1103 94-95 -0.17085004823573513 1103 96-97 -0.0466360359620559 1103 98-99 0.014871752395073656 1103 100-101 0.012897465753415105 1103 102-103 -0.0916390510423497 1103 104-105 -0.06321681962041481 1103 106-107 -0.0905736126084733 1103 108-109 -0.04496400187893457 1103 110-111 0.07334388690906124 1103 112-113 -0.03308707312486092 1103 114-115 -0.09695496225375422 1103 116-117 -0.15821116324123707 1103 118-119 -0.27523147113528523 1103 120-121 -0.33305328556529545 1103 122-123 -0.391706664666458 1103 124-125 -0.44394591174282994 1104 1 -0.04789910926600882 1104 2 0.03321927192094165 1104 3 -0.0395479543582411 1104 4 0.0278173659613401 1104 5 -0.017236508094477188 1104 6 -0.08763080516214217 1104 7 -0.06372470359477944 1104 8 0.013378449041383078 1104 9 0.19111851617437736 1104 10-11 0.09785811553394552 1104 12-13 0.15191954004546204 1104 14-15 0.14093930588023085 1104 16-17 0.03667306833059314 1104 18-19 0.061681632942480746 1104 20-21 0.04627348650494412 1104 22-23 -0.04228624239537737 1104 24-25 0.039451077030527415 1104 26-27 0.11666183763053795 1104 28-29 0.126763650649643 1104 30-31 0.06521730718302621 1104 32-33 0.14925315887045798 1104 34-35 0.06420877612539755 1104 36-37 -0.06617999080699377 1104 38-39 -0.07417862518615692 1104 40-41 0.019968276722764244 1104 42-43 -0.004139774526109363 1104 44-45 0.030387286840479533 1104 46-47 -0.04055580434297923 1104 48-49 0.04690457839794249 1104 50-51 -0.16537066581300763 1104 52-53 0.005725062640010492 1104 54-55 0.08704269361020778 1104 56-57 0.09998170258832317 1104 58-59 -0.061502133444356843 1104 60-61 -0.13614255673486042 1104 62-63 -0.06291456996641642 1104 64-65 0.042955945759402425 1104 66-67 0.060738095273510595 1104 68-69 0.061775531851811394 1104 70-71 -0.06123996254009256 1104 72-73 0.06933669453841418 1104 74-75 0.06066036470702585 1104 76-77 -0.02789986566113356 1104 78-79 0.08060653900138348 1104 80-81 -0.14784483426986128 1104 82-83 0.042235093075198904 1104 84-85 -0.010529990299204428 1104 86-87 0.020915777568319527 1104 88-89 0.026100794944738936 1104 90-91 -0.07722120237195185 1104 92-93 -0.12851912179549174 1104 94-95 -0.14116308166390112 1104 96-97 -0.14655699968893998 1104 98-99 -0.08605152285385742 1104 100-101 -4.0827748982863454E-4 1104 102-103 -0.23802356384462087 1104 104-105 -0.1682094405308021 1104 106-107 -0.13010001730264165 1104 108-109 -0.14468830842232094 1104 110-111 -0.23446381223745405 1104 112-113 -0.24523332134676679 1104 114-115 -0.19316495514138055 1104 116-117 -0.18271791999799447 1104 118-119 -0.4073551375648705 1104 120-121 -0.299499409320628 1104 122-123 -0.39533530975892006 1104 124-125 -0.22744964573998416 1105 1 0.0669422455358415 1105 2 0.08087646762648504 1105 3 0.08783911249094345 1105 4 0.13939877653683652 1105 5 0.016343286119997913 1105 6 6.526632686174594E-4 1105 7 0.01023123748652921 1105 8 0.019820559842010255 1105 9 0.058812719900913635 1105 10-11 0.09418683358854452 1105 12-13 0.061907816832082574 1105 14-15 -0.07951906257088837 1105 16-17 -0.06825075199454034 1105 18-19 -0.03713328311422259 1105 20-21 -0.02232436980155228 1105 22-23 0.009004760568657844 1105 24-25 0.041989987068653534 1105 26-27 0.13563083475134619 1105 28-29 0.1622555377334578 1105 30-31 0.05354040686285799 1105 32-33 -0.15719609333277162 1105 34-35 0.09133152269469491 1105 36-37 0.004772053613940841 1105 38-39 0.02227123156155386 1105 40-41 0.10790651843873178 1105 42-43 0.25434567893174176 1105 44-45 0.19287266817723037 1105 46-47 0.14050526666943597 1105 48-49 0.06612290592387637 1105 50-51 0.07539403345616336 1105 52-53 0.10127225670514406 1105 54-55 0.19292530026458365 1105 56-57 0.21002426464074375 1105 58-59 0.25863996279063883 1105 60-61 0.23991327489207492 1105 62-63 0.012654638709605592 1105 64-65 0.1514456946648579 1105 66-67 0.18518985121006892 1105 68-69 0.02416667849238152 1105 70-71 0.1223323544137358 1105 72-73 0.33183865665645484 1105 74-75 0.18277631633781155 1105 76-77 0.30305880400162266 1105 78-79 0.2762965257650407 1105 80-81 0.24520346989449493 1105 82-83 0.2926520873507634 1105 84-85 0.23314659820328032 1105 86-87 0.1882740171913646 1105 88-89 0.1793191458039729 1105 90-91 0.10741074670772832 1105 92-93 0.07404013416934774 1105 94-95 0.07063280078719814 1105 96-97 -0.023728675472092675 1105 98-99 -0.15675962827510403 1105 100-101 -0.09592798422565707 1105 102-103 -0.07603542917897954 1105 104-105 -0.05118456653059411 1105 106-107 -0.08525881452074557 1105 108-109 0.04306208258714861 1105 110-111 -0.04416249100726688 1105 112-113 -0.13156985605539262 1105 114-115 -0.01987228066489166 1105 116-117 -0.1860202883982076 1105 118-119 -0.013934826425980873 1105 120-121 -0.08165569573471387 1105 122-123 -0.09066637033498282 1105 124-125 0.13796019299158857 1106 1 -0.10361002851859524 1106 2 -0.032097433673541786 1106 3 0.010154525899793043 1106 4 -0.024912099615349348 1106 5 -0.13376067114641188 1106 6 -0.07167213816180862 1106 7 -0.05752822934275059 1106 8 -0.09808162214090999 1106 9 0.03199897997150458 1106 10-11 -0.004156051410774353 1106 12-13 -0.03048995278070521 1106 14-15 0.038277095058298016 1106 16-17 -0.0314360129729323 1106 18-19 0.06445906315047267 1106 20-21 -0.06880739298566141 1106 22-23 -0.021080661814792734 1106 24-25 0.0531206493806593 1106 26-27 0.11435241014749664 1106 28-29 0.17129603025146878 1106 30-31 -0.05714324781641977 1106 32-33 -0.2372479255472939 1106 34-35 -0.06027942735585867 1106 36-37 0.013660787471280855 1106 38-39 -0.07146532160479069 1106 40-41 -0.04511625225065785 1106 42-43 -0.07291601604541142 1106 44-45 -0.0886993345030902 1106 46-47 0.03636764688602767 1106 48-49 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2216 120-121 -1.4339916274622517 2216 122-123 -1.3275877922876234 2216 124-125 -1.2589993765393857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43241.0 3 6667.0 4 4555.0 5 3179.0 6 3606.0 7 3459.0 8 4163.0 9 4802.0 10 6199.0 11 7802.0 12 9120.0 13 10421.0 14 10695.0 15 10960.0 16 11236.0 17 11738.0 18 11940.0 19 12678.0 20 13375.0 21 14485.0 22 16092.0 23 18623.0 24 21385.0 25 23132.0 26 26324.0 27 28112.0 28 32811.0 29 40772.0 30 47801.0 31 57637.0 32 71453.0 33 86830.0 34 103259.0 35 122831.0 36 171562.0 37 274385.0 38 127468.0 39 696.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.32005491886119 16.48553340216352 9.718813251354584 22.4755984276207 2 12.927423687656493 23.0331275276006 37.4654503087086 26.573998476034312 3 22.768446825373047 32.419854729462905 25.618638967037693 19.193059478126354 4 13.372096981746134 19.044412519659843 33.012670810836575 34.57081968775745 5 11.831050934055423 42.13561216937665 31.223875759768994 14.809461136798932 6 30.148525840854273 28.513325243809266 22.445773632237163 18.892375283099295 7 26.54269757701318 33.00379151243152 21.48185568287733 18.97165522767797 8 29.779244261474496 32.08766212760451 21.714355763042565 16.418737847878422 9 32.88333295873033 16.556839876285654 18.796352865134065 31.763474299849953 10-11 23.86672315568355 30.59523939264317 23.81148289118417 21.726554560489113 12-13 27.663918514472073 27.128444328802882 23.30244159608974 21.905195560635306 14-15 28.498424594708517 25.45806602742506 22.68450832169456 23.359001056171863 16-17 23.665473944485132 28.53827010680022 29.221046524336742 18.575209424377906 18-19 26.08786978380822 27.53691029321248 28.028843273216513 18.34637664976278 20-21 22.36321628064862 28.71366887271108 28.68959736227998 20.233517484360327 22-23 20.99510992453755 30.97655079412646 21.95749448334105 26.070844797994937 24-25 21.491158180317555 32.56896946221797 21.140726848787452 24.79914550867702 26-27 24.50441063046221 26.864510812472016 22.105456718613524 26.525621838452253 28-29 23.96398224718688 27.36451630258236 25.33678110743285 23.33472034279791 30-31 27.00246390557129 28.894648029301234 24.166401318623688 19.936486746503792 32-33 20.00706978036685 29.468383846845946 27.09558029751652 23.428966075270687 34-35 17.878140150371024 30.967175338307563 27.594536953071287 23.560147558250126 36-37 24.749948006885216 28.214374334768944 24.846003371808806 22.189674286537034 38-39 27.70217624887125 26.450021673345596 23.73064624676856 22.117155831014593 40-41 22.349234366154246 30.85959614379654 23.903569056743457 22.887600433305753 42-43 25.99962435614848 28.503508391080633 23.16504443417624 22.33182281859464 44-45 27.77335864323153 26.303335521911137 23.547191081222106 22.376114753635235 46-47 23.403805561597586 27.77936062017467 29.709509274605455 19.107324543622287 48-49 25.81679279141898 27.135248033243563 27.934544929730492 19.113414245606965 50-51 21.261997934799847 28.889999162756695 28.753964142843238 21.09403875960022 52-53 20.290441505528644 30.908620031901467 22.41600341407619 26.384935048493695 54-55 21.077672638762397 32.04699128621272 21.547156686174333 25.328179388850547 56-57 24.103277790443265 26.74827399646803 22.42218948099099 26.726258732097712 58-59 23.751566135672096 26.93273562132626 25.42148700711388 23.894211235887767 60-61 26.43776616634031 28.632198529717044 24.380207195415863 20.549828108526782 62-63 19.776914959037665 29.24312328586242 26.97368085212823 24.00628090297169 64-65 17.644353891166336 30.389470617306806 27.738915203193475 24.227260288333376 66-67 24.117675686363796 28.120198193065693 24.932623682635167 22.829502437935343 68-69 26.82854113879355 26.4223858475267 23.94760889430399 22.80146411937576 70-71 22.00386661538409 30.191492765412853 24.097489082597846 23.707151536605206 72-73 25.375504214966526 28.139985833818642 23.502688749390312 22.981821201824516 74-75 27.16028203091121 25.853196606751776 23.726902200737005 23.25961916160001 76-77 22.910307759749717 27.370108122973203 29.707168082076528 20.01241603520055 78-79 25.227532379313317 26.969652933783422 27.88475118713655 19.91806349976671 80-81 20.853805803221956 28.729529231896073 28.57555175263402 21.841113212247947 82-83 20.00731063315937 30.451714084812874 22.6316849920325 26.90929028999526 84-85 20.786258346890293 31.514011884746544 21.86327095996896 25.836458808394198 86-87 23.52180168295126 26.310757498247987 22.860581480320473 27.306859338480276 88-89 23.233824428761668 26.421072441879023 25.697820954758665 24.64728217460064 90-91 25.57802686233436 28.200550315401856 24.661106790796765 21.560316031467014 92-93 19.70098086933791 28.699396801776444 26.839223609482076 24.760398719403568 94-95 17.693723031389265 29.656095118745274 27.76003033213552 24.890151517729937 96-97 23.802093363665623 27.461566885629885 25.278887186875 23.457452563829488 98-99 26.14760505582318 26.117467135720645 24.189796783338288 23.545131025117882 100-101 21.677068579149097 29.119266192355788 24.379218761267605 24.824446467227514 102-103 24.792638262980493 27.385012819714916 24.017299728301627 23.805049189002965 104-105 26.18951339317446 25.403448103468275 24.2322914247021 24.174747078655162 106-107 22.282895242604774 26.963924781328814 29.629716559142416 21.123463416923997 108-109 24.323195266755295 26.8715462377209 27.721370768567137 21.08388772695666 110-111 20.31563346151569 28.606804530183645 28.550527688954432 22.527034319346235 112-113 19.553230768393266 29.93590655244755 23.14713185394669 27.363730825212496 114-115 20.090255053541306 31.433173223154665 22.388138918204998 26.088432805099032 116-117 22.632352125855054 26.729743399442928 23.207144720591753 27.43075975411027 118-119 22.26295099533232 26.89485520597795 25.569640245307557 25.27255355338217 120-121 24.26206896082194 28.42578683085294 24.815472561468578 22.49667164685654 122-123 19.350527481216798 28.9160283110734 26.437210239185156 25.29623396852465 124-125 17.87917512135972 29.99514152849963 27.09162641756453 25.03405693257612 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6754.0 1 3921.5 2 1813.5 3 2583.0 4 2464.5 5 2012.5 6 1484.5 7 1236.0 8 1204.5 9 1117.0 10 993.5 11 910.5 12 897.0 13 907.5 14 952.5 15 1012.5 16 1030.0 17 1081.5 18 1213.0 19 1382.5 20 1577.5 21 1901.5 22 2325.0 23 2907.0 24 3562.0 25 4344.5 26 5396.0 27 6581.5 28 7737.0 29 9086.5 30 11088.5 31 13006.5 32 14727.5 33 16532.5 34 18670.0 35 20326.0 36 22004.0 37 23783.5 38 24208.5 39 24277.0 40 24631.5 41 26454.0 42 28142.0 43 29296.0 44 31157.5 45 32862.5 46 36177.5 47 44704.5 48 56382.5 49 90166.5 50 211927.5 51 231268.0 52 109293.0 53 50379.5 54 34703.5 55 27573.0 56 22928.5 57 19570.0 58 17359.0 59 15575.5 60 13551.5 61 11413.0 62 9641.0 63 7708.5 64 6064.5 65 4797.0 66 3440.0 67 2751.0 68 2140.5 69 1423.5 70 1150.5 71 1023.0 72 954.0 73 878.5 74 653.0 75 516.5 76 379.0 77 220.0 78 152.5 79 108.0 80 71.0 81 46.0 82 37.0 83 34.5 84 29.5 85 20.5 86 16.5 87 17.0 88 17.5 89 14.5 90 10.0 91 8.0 92 5.0 93 3.0 94 2.0 95 1.5 96 2.0 97 1.0 98 0.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48431237266976346 2 0.4715031033674146 3 0.4599137644748132 4 0.4584227384184551 5 0.46025263403307637 6 0.46953765992948804 7 0.45320414722120184 8 0.4831602161716686 9 0.4929196594496487 10-11 0.4989515375867336 12-13 0.4985448941168178 14-15 0.4732652250703832 16-17 0.47472236417091496 18-19 0.4607270514146449 20-21 0.47123200772080404 22-23 0.488920998662143 24-25 0.47563731197822556 26-27 0.4857017378586426 28-29 0.4816691901153105 30-31 0.4945123463734858 32-33 0.4929196594496487 34-35 0.48746385956161126 36-37 0.4438852343689638 38-39 0.4030514525982485 40-41 0.3972906701077741 42-43 0.40779562641393324 44-45 0.4407676344329424 46-47 0.44849386036134337 48-49 0.4458167908510642 50-51 0.43287197372540986 52-53 0.42578959995770904 54-55 0.42887331293790415 56-57 0.4106421307033441 58-59 0.4143019219325867 60-61 0.42379026956395627 62-63 0.4255185043110985 64-65 0.44053042574215817 66-67 0.42836500860050936 68-69 0.4304659998617412 70-71 0.44114039094703195 72-73 0.44069986052128984 74-75 0.4457490169394115 76-77 0.43551515627986287 78-79 0.42748394774902504 80-81 0.43700618233622096 82-83 0.43382080848854687 84-85 0.432668651990452 86-87 0.43832777361344744 88-89 0.4266706608091934 90-91 0.42944939118695163 92-93 0.4399204605372845 94-95 0.4354473823682103 96-97 0.4061690525342699 98-99 0.37851729657999283 100-101 0.3776362357285086 102-103 0.35903229697985894 104-105 0.3607944186828276 106-107 0.379059487873214 108-109 0.38766677465309923 110-111 0.40481357430121706 112-113 0.40128933089527985 114-115 0.39857837442917426 116-117 0.37177379237055524 118-119 0.4447662952204482 120-121 0.45235697332554387 122-123 0.40413583518469065 124-125 0.39959498310396385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1475494.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.865386990961646 #Duplication Level Percentage of deduplicated Percentage of total 1 88.41852559241165 45.85861047020677 2 8.094428057853758 8.396412873821667 3 1.7332528090583321 2.6968748308494526 4 0.6257959293014301 1.2982859220234861 5 0.28843453117965623 0.747988429059473 6 0.17383573950887776 0.5409634741492769 7 0.12721893343085397 0.46187814504780417 8 0.09071287482535344 0.3763886686303695 9 0.06564585102483068 0.3064272720978475 >10 0.3361173182176843 3.191417888424603 >50 0.021997327380039056 0.7917557452749752 >100 0.015704713046668747 1.5825272483299937 >500 0.0028678160324238345 1.08771721130751 >1k 0.001775314686738564 2.0657915077811317 >5k 0.0021850026913705404 7.6520555213139385 >10k+ 0.0015021893503172467 22.944904791681747 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 85356 5.784910003022716 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 56374 3.820686495505912 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 38829 2.631593215560348 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 26835 1.8187129191985871 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 23786 1.6120702625696883 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 20343 1.37872468474965 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 17377 1.1777072627879204 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 16602 1.1251824812571247 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 15369 1.04161724818942 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 12285 0.832602504652679 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 10793 0.7314838284669406 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 9220 0.6248754654373382 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 8995 0.6096263353154943 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 8818 0.5976303529529772 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 8270 0.5604902493673305 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 8006 0.5425979366910336 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 7841 0.5314152412683482 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 6462 0.4379550170993579 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 6201 0.42026602615801895 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 6045 0.4096932959402072 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5896 0.39959498310396385 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 5594 0.37912726178486666 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 5493 0.37228209670795004 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5475 0.3710621662982025 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 5412 0.3667924098640862 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 5251 0.3558808100880112 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 5057 0.34273267122739914 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 4404 0.29847630691822535 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 4026 0.27285776831352754 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 3959 0.2683169162328007 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 2957 0.20040745675685567 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 2760 0.18705599616128563 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1971 0.1335823798673529 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 1584 0.10735387605778132 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.710956466105589E-4 0.0 12-13 0.0 0.0 0.0 0.0010166086747895957 0.0 14-15 0.0 0.0 0.0 0.002168765172884471 0.0 16-17 0.0 0.0 0.0 0.0038631129642004644 0.0 18-19 0.0 0.0 0.0 0.00515081728560062 0.0 20-21 0.0 0.0 0.0 0.007150147679353491 0.0 22-23 0.0 0.0 0.0 0.010708278041117077 0.0 24-25 0.0 0.0 0.0 0.014232521447054343 0.0 26-27 0.0 0.0 0.0 0.01796008658794953 0.0 28-29 0.0 0.0 0.0 0.023077016917723826 0.0 30-31 0.0 0.0 0.0 0.03022716459707732 0.0 32-33 0.0 0.0 0.0 0.04303643389942623 0.0 34-35 0.0 0.0 0.0 0.06143705091311791 0.0 36-37 0.0 0.0 0.0 0.0873266851644263 0.0 38-39 0.0 0.0 0.0 0.13073587557794203 0.0 40-41 0.0 0.0 0.0 0.20549050013080367 0.0 42-43 0.0 0.0 0.0 0.26038736856944183 0.0 44-45 0.0 0.0 0.0 0.31972342822132793 0.0 46-47 0.0 0.0 0.0 0.39691791359368456 0.0 48-49 0.0 0.0 0.0 0.46482737306962957 0.0 50-51 0.0 0.0 0.0 0.5378537628753488 0.0 52-53 0.0 0.0 0.0 0.6358209521692396 0.0 54-55 0.0 0.0 0.0 0.7371090631341097 0.0 56-57 0.0 0.0 0.0 0.8507659129755865 0.0 58-59 0.0 0.0 0.0 0.9821795276700549 0.0 60-61 0.0 0.0 0.0 1.107595151183265 0.0 62-63 0.0 0.0 0.0 1.2343662529295274 0.0 64-65 0.0 0.0 0.0 1.3829944411837662 0.0 66-67 0.0 0.0 0.0 1.544431898740354 0.0 68-69 0.0 0.0 0.0 1.753006111851353 0.0 70-71 0.0 0.0 0.0 2.0647999924093217 0.0 72-73 0.0 0.0 0.0 2.3041774483664454 0.0 74-75 0.0 0.0 0.0 2.5424705217371266 0.0 76-77 0.0 0.0 0.0 2.820784089938692 0.0 78-79 0.0 0.0 0.0 3.0895415365972347 0.0 80-81 6.777391165263972E-5 0.0 0.0 3.36877005260611 0.0 82-83 6.777391165263972E-5 0.0 0.0 3.726074114838827 0.0 84-85 6.777391165263972E-5 0.0 0.0 4.0694167512711 0.0 86-87 6.777391165263972E-5 0.0 0.0 4.410726170353794 0.0 88-89 6.777391165263972E-5 0.0 0.0 4.826722440077695 0.0 90-91 6.777391165263972E-5 0.0 0.0 5.202156023677494 0.0 92-93 6.777391165263972E-5 0.0 0.0 5.578267346393818 0.0 94-95 6.777391165263972E-5 0.0 0.0 6.012528685308107 0.0 96-97 6.777391165263972E-5 0.0 0.0 6.453770737122618 0.0 98-99 6.777391165263972E-5 0.0 0.0 6.963295004927163 0.0 100-101 6.777391165263972E-5 0.0 0.0 7.7148060242874585 0.0 102-103 6.777391165263972E-5 0.0 0.0 8.318874898847437 0.0 104-105 6.777391165263972E-5 0.0 0.0 8.890276747990843 0.0 106-107 6.777391165263972E-5 0.0 0.0 9.500343613732078 0.0 108-109 6.777391165263972E-5 0.0 0.0 10.046059150359135 0.0 110-111 6.777391165263972E-5 0.0 0.0 10.60417731281862 0.0 112-113 6.777391165263972E-5 0.0 0.0 11.29784329858339 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACTC 1535 0.0 60.500526 5 AAGTACT 1810 0.0 51.30671 4 AAAAAGT 2350 0.0 38.751854 1 AAAGTAC 2495 0.0 37.219227 3 AAAAGTA 2515 0.0 36.684063 2 ATAACGC 175 1.6647391E-8 30.613836 3 TATAACG 185 2.8598151E-8 28.957062 2 ATGGGGG 2155 0.0 27.072086 5 GCGCGAC 110 7.728736E-4 27.059528 9 CATGGGG 6515 0.0 24.5791 4 GAACAAA 1250 0.0 23.33216 1 CATGGGT 4085 0.0 22.150301 4 GTGTTAA 480 0.0 21.080292 1 GTACAAA 1280 0.0 20.925287 1 ATGGGAT 2625 0.0 20.183832 5 TAACGCA 325 1.447188E-8 20.148252 4 ATGGGGA 3670 0.0 19.951794 5 TGGGGGG 1230 0.0 19.842978 6 GTACTTG 975 0.0 18.924568 1 TGGGGGA 1170 0.0 18.82539 6 >>END_MODULE