FastQCFastQC Report
Thu 26 May 2016
SRR1364152_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364152_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2389579
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA398721.6685784399678771No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT294871.2339830572665729No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG199910.8365908806530354No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG99300.4155543717115023No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC90880.3803180392864182No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN87190.36487598861556786No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG76070.3183405947240079No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT70540.2951984429056332No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC48890.2045967092948172No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT43120.1804501964572002No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA40630.17002995088256134No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG38070.1593167666773101No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG36300.15190960416039812No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC35580.14889652110267124No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG29980.1254614306536842No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA27720.11600369772248585No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG25520.10679705504609807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG78700.024.9615784
TAGGCCG1350.002548711422.0479955
ATGGGGG33900.021.072425
GTACACG4605.456968E-1219.4105971
GTCCAAG18050.018.1380631
GTATAGG7550.018.1336981
ATGGGGA45400.018.0948965
CGGTTAC5100.018.08947228-29
ATGGGAG27700.018.0522925
CTCGTAA2652.077668E-517.970061
AGTACTC13000.017.4009555
GTGTAGC12500.017.1434381
TCTATAC5751.0913936E-1116.5647543
CATGGGT42200.016.5046464
ATGGGTA20700.016.3922045
CCAAGAC17750.016.098143
ACTTGAC12250.016.034188
CGCGGAA5050.015.90448582-83
ATGGGAT32150.015.7387715
ATTCGCG5200.015.44618880-81
Produced by FastQC (version 0.11.5)