##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364152_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2389579 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.688607491110357 31.0 31.0 34.0 27.0 34.0 2 30.817750741867084 31.0 31.0 34.0 27.0 34.0 3 30.90569175574442 33.0 31.0 34.0 27.0 34.0 4 34.38158102326812 37.0 35.0 37.0 30.0 37.0 5 34.35559778521656 37.0 35.0 37.0 31.0 37.0 6 34.34124421080031 37.0 35.0 37.0 30.0 37.0 7 34.26163102370752 37.0 35.0 37.0 30.0 37.0 8 34.220132918811224 37.0 35.0 37.0 30.0 37.0 9 35.69961319546246 39.0 37.0 39.0 30.0 39.0 10-11 35.61340700600398 39.0 37.0 39.0 30.0 39.0 12-13 35.5256997153055 39.0 37.0 39.0 29.5 39.0 14-15 36.764218090299586 40.0 37.0 41.0 29.5 41.0 16-17 36.789864239684064 40.0 37.0 41.0 30.0 41.0 18-19 36.79095752850188 40.0 37.0 41.0 30.0 41.0 20-21 36.798954962359474 40.0 37.0 41.0 30.0 41.0 22-23 36.75416171635254 40.0 37.0 41.0 30.0 41.0 24-25 36.71072854255917 40.0 37.0 41.0 29.5 41.0 26-27 36.600224139900796 40.0 37.0 41.0 29.0 41.0 28-29 36.53250237803395 40.0 37.0 41.0 29.0 41.0 30-31 36.63251497439507 40.0 37.0 41.0 30.0 41.0 32-33 36.466710244775335 40.0 36.0 41.0 29.5 41.0 34-35 36.54357817841553 40.0 37.0 41.0 30.0 41.0 36-37 36.52450515341823 40.0 36.5 41.0 30.0 41.0 38-39 36.45711001812453 40.0 36.0 41.0 30.0 41.0 40-41 36.334218914712594 40.0 36.0 41.0 30.0 41.0 42-43 36.22258355969817 40.0 36.0 41.0 29.0 41.0 44-45 36.08668807350583 40.0 35.5 41.0 29.0 41.0 46-47 35.93422920941304 40.0 35.0 41.0 28.0 41.0 48-49 35.80029369190137 39.5 35.0 41.0 28.0 41.0 50-51 35.68990876635591 39.0 35.0 41.0 28.0 41.0 52-53 35.54183812294969 39.0 35.0 41.0 27.5 41.0 54-55 35.35036569203194 39.0 35.0 41.0 27.0 41.0 56-57 35.116884396791235 39.0 34.5 41.0 26.0 41.0 58-59 34.878644941221864 38.5 34.0 40.5 26.0 41.0 60-61 34.63909814239244 38.0 34.0 40.0 26.0 41.0 62-63 34.302027051627086 38.0 34.0 40.0 25.5 41.0 64-65 34.05241864780365 37.0 33.0 40.0 25.0 41.0 66-67 33.73953424431667 37.0 33.0 40.0 24.5 41.0 68-69 33.398585692291405 36.0 33.0 39.0 23.0 41.0 70-71 33.137640144979514 36.0 33.0 39.0 23.0 41.0 72-73 32.9833353490301 35.5 33.0 39.0 24.0 40.5 74-75 32.687504786407985 35.0 33.0 37.5 23.0 40.0 76-77 32.26426809910867 35.0 33.0 37.0 21.5 39.0 78-79 31.91307255378458 35.0 32.0 37.0 20.5 39.0 80-81 31.665291668532404 35.0 32.0 36.0 20.0 38.5 82-83 31.41860595527497 35.0 32.0 36.0 20.0 37.0 84-85 31.177875893619756 35.0 32.0 35.5 20.0 37.0 86-87 30.923898310120734 35.0 32.0 35.0 18.0 36.5 88-89 30.70612229183467 35.0 32.0 35.0 16.0 36.0 90-91 30.551701575884287 35.0 32.0 35.0 12.5 36.0 92-93 30.368407782291357 35.0 32.0 35.0 10.0 36.0 94-95 30.292063581074324 35.0 32.0 35.0 7.0 36.0 96-97 30.227015721179335 35.0 32.0 35.0 7.0 35.0 98-99 30.10653069013412 35.0 32.0 35.0 3.5 35.0 100-101 29.9040163560192 35.0 31.0 35.0 2.0 35.0 102-103 28.84368146020701 33.5 29.5 34.5 2.0 35.0 104-105 29.617445374268854 34.5 31.0 35.0 2.0 35.0 106-107 29.647829387519728 35.0 31.0 35.0 2.0 35.0 108-109 29.597629540601083 35.0 31.0 35.0 2.0 35.0 110-111 29.604365036686378 35.0 31.0 35.0 2.0 35.0 112-113 29.51921656492629 35.0 31.0 35.0 2.0 35.0 114-115 29.416276465435963 35.0 31.0 35.0 2.0 35.0 116-117 29.248440415654805 35.0 31.0 35.0 2.0 35.0 118-119 29.091049929715652 35.0 31.0 35.0 2.0 35.0 120-121 28.92786658235614 35.0 31.0 35.0 2.0 35.0 122-123 28.677540478887703 35.0 30.0 35.0 2.0 35.0 124-125 27.76752578592296 34.0 27.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.31813738740575914 1101 2 0.38698451257881317 1101 3 0.4254988908928148 1101 4 0.4395757391164494 1101 5 0.3217753083500128 1101 6 0.38917713003577603 1101 7 0.39634060543600214 1101 8 0.43202014049232673 1101 9 0.5718330619179852 1101 10-11 0.49534716896882003 1101 12-13 0.4861475224387135 1101 14-15 0.5742111909365732 1101 16-17 0.5757773303391147 1101 18-19 0.5396049800779963 1101 20-21 0.6135241164817842 1101 22-23 0.6033668319479304 1101 24-25 0.5846012290050524 1101 26-27 0.687457313302346 1101 28-29 0.8263886113341385 1101 30-31 0.6690784644364101 1101 32-33 0.7984242005261137 1101 34-35 0.6840848447113572 1101 36-37 0.6515533843699401 1101 38-39 0.626756815052893 1101 40-41 0.6367900697943796 1101 42-43 0.6578759445181817 1101 44-45 0.6123859424201754 1101 46-47 0.6855093126369027 1101 48-49 0.8333992987769037 1101 50-51 0.7531197213844081 1101 52-53 0.8062109048774246 1101 54-55 0.6384243130337239 1101 56-57 0.6972853771826593 1101 58-59 0.7139754795856845 1101 60-61 0.7606293850846626 1101 62-63 0.7191625568449211 1101 64-65 0.7252340078325616 1101 66-67 0.6949323556618552 1101 68-69 0.7489141588135197 1101 70-71 0.7557405724400184 1101 72-73 0.8137274966271306 1101 74-75 0.7510734599486995 1101 76-77 0.6787125146389741 1101 78-79 0.7497365589958882 1101 80-81 0.7537626316571604 1101 82-83 0.7914974884580914 1101 84-85 0.7214783133853437 1101 86-87 0.6443642698398442 1101 88-89 0.6206072263237523 1101 90-91 0.6226679298024926 1101 92-93 0.6141684439262676 1101 94-95 0.6493502192959397 1101 96-97 0.6152764260237014 1101 98-99 0.6526043268401054 1101 100-101 0.5592655603020837 1101 102-103 0.6477719084055984 1101 104-105 0.7209949612948456 1101 106-107 0.6694047524538931 1101 108-109 0.6139634720346692 1101 110-111 0.6357504718625293 1101 112-113 0.7813725243351541 1101 114-115 0.706337120796114 1101 116-117 0.7774502405792383 1101 118-119 0.690871786891357 1101 120-121 0.6160751589746027 1101 122-123 0.6605894256237796 1101 124-125 0.6802753878742429 1102 1 0.1733494991559965 1102 2 0.2630256879879198 1102 3 0.2901836175897188 1102 4 0.34881903858300234 1102 5 0.24540988774568007 1102 6 0.27875339314639547 1102 7 0.3397970446187344 1102 8 0.3268256886698637 1102 9 0.4136868879991269 1102 10-11 0.48593594812240326 1102 12-13 0.4531921507590724 1102 14-15 0.5450235273389339 1102 16-17 0.47715383148366186 1102 18-19 0.5507392097219395 1102 20-21 0.5660746277275663 1102 22-23 0.4866854385370445 1102 24-25 0.6056885615043939 1102 26-27 0.6627867388782178 1102 28-29 0.5936065948583007 1102 30-31 0.4676878921209351 1102 32-33 0.5560968612953303 1102 34-35 0.5386615234689529 1102 36-37 0.5429651445436647 1102 38-39 0.4486174536894012 1102 40-41 0.4878881280133456 1102 42-43 0.46886755655937407 1102 44-45 0.4432618221217979 1102 46-47 0.5380100678978792 1102 48-49 0.5460303036085463 1102 50-51 0.5492732634063131 1102 52-53 0.525832443095652 1102 54-55 0.496014655425455 1102 56-57 0.6200771867068511 1102 58-59 0.6634919706330606 1102 60-61 0.6660904186871903 1102 62-63 0.5757718912623204 1102 64-65 0.5982222663122698 1102 66-67 0.6331103620567333 1102 68-69 0.5252606826868629 1102 70-71 0.650177593812387 1102 72-73 0.5764995578509158 1102 74-75 0.6209614380187531 1102 76-77 0.5688342282399645 1102 78-79 0.6141100167935285 1102 80-81 0.6023976672507345 1102 82-83 0.616828150837172 1102 84-85 0.6828956087603082 1102 86-87 0.6503907455386901 1102 88-89 0.6763242781904104 1102 90-91 0.6478466884170295 1102 92-93 0.7007175399447725 1102 94-95 0.6920203760455408 1102 96-97 0.6555334377854969 1102 98-99 0.5787241913189156 1102 100-101 0.6217836510834402 1102 102-103 0.6070680254517882 1102 104-105 0.6337627443960585 1102 106-107 0.658008077224224 1102 108-109 0.5601798882655871 1102 110-111 0.6069775225734908 1102 112-113 0.6062354723468388 1102 114-115 0.6745699317659515 1102 116-117 0.650317211242676 1102 118-119 0.6149933423437375 1102 120-121 0.6629300495175201 1102 122-123 0.6484659967186737 1102 124-125 0.6429410434711826 1103 1 0.32295942680060463 1103 2 0.35532408323429365 1103 3 0.434196310099523 1103 4 0.4178145517403635 1103 5 0.3296703671506691 1103 6 0.36828894430044556 1103 7 0.40052544916216704 1103 8 0.36713168325331935 1103 9 0.5116415345086835 1103 10-11 0.5167227624641342 1103 12-13 0.5663602254589435 1103 14-15 0.5822651296672419 1103 16-17 0.5308595188598701 1103 18-19 0.46994228987877307 1103 20-21 0.49100954829832943 1103 22-23 0.40328311831391517 1103 24-25 0.5792974381768516 1103 26-27 0.6730347278834401 1103 28-29 0.7453968854925179 1103 30-31 0.553492896365249 1103 32-33 0.6027662901323936 1103 34-35 0.4436091769715276 1103 36-37 0.4715065164665404 1103 38-39 0.5127605071677195 1103 40-41 0.49797786486608686 1103 42-43 0.49923236516117697 1103 44-45 0.49280202180651145 1103 46-47 0.4778780900491384 1103 48-49 0.38801259877003247 1103 50-51 0.4008264789768745 1103 52-53 0.49466768300025166 1103 54-55 0.48988486036502366 1103 56-57 0.4381888727087144 1103 58-59 0.525635347487416 1103 60-61 0.47766364315058496 1103 62-63 0.5320953077302448 1103 64-65 0.3780743217712583 1103 66-67 0.4844081105510085 1103 68-69 0.42270378871377545 1103 70-71 0.49174556341503717 1103 72-73 0.4729003824598337 1103 74-75 0.4352494043876476 1103 76-77 0.3094161030125875 1103 78-79 0.35616486945532344 1103 80-81 0.4183489991558247 1103 82-83 0.4311791755350214 1103 84-85 0.40623204111408384 1103 86-87 0.38953238424680237 1103 88-89 0.4311565268665909 1103 90-91 0.3971441539572673 1103 92-93 0.3879344848495805 1103 94-95 0.4145752489183323 1103 96-97 0.4268992973594443 1103 98-99 0.5095904349019236 1103 100-101 0.4770023341344931 1103 102-103 0.3789911023748651 1103 104-105 0.5041427112500152 1103 106-107 0.39438141486923683 1103 108-109 0.3554901271596371 1103 110-111 0.4140489482576335 1103 112-113 0.48997935851847174 1103 114-115 0.4444019783321025 1103 116-117 0.4552830546478255 1103 118-119 0.44319213776796573 1103 120-121 0.30877992218820083 1103 122-123 0.34699241249942503 1103 124-125 0.400230950096347 1104 1 0.15856093201843535 1104 2 0.2129397224992715 1104 3 0.18945099450810332 1104 4 0.23255669913274346 1104 5 0.1537929699878262 1104 6 0.19104598869402878 1104 7 0.25013621328005087 1104 8 0.18908019032033963 1104 9 0.34289872761365103 1104 10-11 0.21534572738117674 1104 12-13 0.24569516228893207 1104 14-15 0.2469679978572259 1104 16-17 0.21406580094596706 1104 18-19 0.2751861442192052 1104 20-21 0.25975526476391053 1104 22-23 0.25338753956800986 1104 24-25 0.2969997503155781 1104 26-27 0.3893443255868121 1104 28-29 0.36774044758275437 1104 30-31 0.3542495463503741 1104 32-33 0.3599073461125286 1104 34-35 0.23087867432704456 1104 36-37 0.3118087561892864 1104 38-39 0.3890672276291056 1104 40-41 0.44206109151516415 1104 42-43 0.4260104360512429 1104 44-45 0.5015443114445617 1104 46-47 0.45690671375761127 1104 48-49 0.38400628176908924 1104 50-51 0.43803439042248016 1104 52-53 0.48259328851421657 1104 54-55 0.4957634860132103 1104 56-57 0.46647426278513393 1104 58-59 0.4291239225737371 1104 60-61 0.437940084681145 1104 62-63 0.358091512357106 1104 64-65 0.35907046174100543 1104 66-67 0.37040612557732544 1104 68-69 0.4521412070841606 1104 70-71 0.3108761514069158 1104 72-73 0.3468841341247568 1104 74-75 0.34807349821806355 1104 76-77 0.3567624130305589 1104 78-79 0.3345883024899443 1104 80-81 0.43746309958126517 1104 82-83 0.38876022323904635 1104 84-85 0.3694466639690823 1104 86-87 0.3503021596279545 1104 88-89 0.3673423708360204 1104 90-91 0.3576522296319631 1104 92-93 0.3276439803947042 1104 94-95 0.32330493041734343 1104 96-97 0.3775888655791775 1104 98-99 0.318173668016712 1104 100-101 0.3156946114876895 1104 102-103 0.14048439096547227 1104 104-105 0.3231350946408931 1104 106-107 0.2912967851605863 1104 108-109 0.25671166186111805 1104 110-111 0.36249992522676067 1104 112-113 0.31368427633926643 1104 114-115 0.3298743309461898 1104 116-117 0.3163025681676288 1104 118-119 0.30718022146014334 1104 120-121 0.39346297296990684 1104 122-123 0.34663275725120357 1104 124-125 0.41700187637429664 1105 1 0.18757361552603413 1105 2 0.32986202761395944 1105 3 0.21224050205003664 1105 4 0.19755439478616665 1105 5 0.13919181397395874 1105 6 0.21332685973703036 1105 7 0.273179198945094 1105 8 0.20099798275631997 1105 9 0.30269316069638563 1105 10-11 0.2614582227004689 1105 12-13 0.2482532270218698 1105 14-15 0.3722698432547773 1105 16-17 0.32814775887243997 1105 18-19 0.2659669576535464 1105 20-21 0.3761871761233735 1105 22-23 0.39522991266092333 1105 24-25 0.4227687059665044 1105 26-27 0.5146910577353268 1105 28-29 0.49340270718660406 1105 30-31 0.42847242947199504 1105 32-33 0.2908311970597097 1105 34-35 0.3252187430733855 1105 36-37 0.3634857684119268 1105 38-39 0.3792694340409568 1105 40-41 0.3857990297010687 1105 42-43 0.4285428169213219 1105 44-45 0.40908312528216584 1105 46-47 0.38122825171692654 1105 48-49 0.3378277765218982 1105 50-51 0.4201507407215601 1105 52-53 0.3631306813380988 1105 54-55 0.35076559833090215 1105 56-57 0.3723588678297389 1105 58-59 0.39067686013554237 1105 60-61 0.36873635644042224 1105 62-63 0.40452805641333356 1105 64-65 0.3927997346236438 1105 66-67 0.36341879468275806 1105 68-69 0.3580273723634235 1105 70-71 0.36716089663259766 1105 72-73 0.29162986388251255 1105 74-75 0.2533739754098292 1105 76-77 0.27012318249028766 1105 78-79 0.17435952408166244 1105 80-81 0.1940946969735151 1105 82-83 0.2240306479109151 1105 84-85 0.1644332699546247 1105 86-87 0.14948735708165017 1105 88-89 0.15759582495551783 1105 90-91 0.10445062811109551 1105 92-93 0.047894626091814274 1105 94-95 0.10492872663629527 1105 96-97 0.14548571726566806 1105 98-99 0.16006716879923033 1105 100-101 0.13006761318714766 1105 102-103 -0.007024634391942897 1105 104-105 0.0918051026100919 1105 106-107 0.18083109112020423 1105 108-109 0.18326721581642857 1105 110-111 0.22919527620755176 1105 112-113 0.21426012294659813 1105 114-115 0.23294403262932306 1105 116-117 0.07303798117374427 1105 118-119 0.17398550897538811 1105 120-121 0.14408634672531662 1105 122-123 0.11712211885118862 1105 124-125 0.17160396852895943 1106 1 0.03049993530588324 1106 2 -0.006838796071175324 1106 3 -0.008291145689881319 1106 4 0.029272603709465272 1106 5 -0.117204781806862 1106 6 -0.033623114348309 1106 7 -0.048468312893461984 1106 8 -0.0548147269892425 1106 9 0.02288890093945639 1106 10-11 0.07080960113239598 1106 12-13 0.07012960582736838 1106 14-15 0.0074110683301071845 1106 16-17 0.02276234611361616 1106 18-19 0.0971111115968597 1106 20-21 0.0791101848038025 1106 22-23 0.044487235420760385 1106 24-25 0.05940354360859601 1106 26-27 0.153497457516373 1106 28-29 0.26556025417314544 1106 30-31 0.06876044793236247 1106 32-33 -0.2015016808991419 1106 34-35 0.008897225095530814 1106 36-37 0.07436689836982424 1106 38-39 0.010065686599979529 1106 40-41 0.10683221855720859 1106 42-43 0.07766498233316099 1106 44-45 0.11955249132066115 1106 46-47 0.10271252871358882 1106 48-49 0.047523477833991024 1106 50-51 0.11269184043127467 1106 52-53 0.15563929924115172 1106 54-55 0.17314894499515532 1106 56-57 0.09742860933589981 1106 58-59 0.17000411709022956 1106 60-61 0.22311833304880224 1106 62-63 0.1372679872498921 1106 64-65 0.19277098861800113 1106 66-67 0.09899490631949703 1106 68-69 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-2.2260387932106056 2216 100-101 -2.3599933224834153 2216 102-103 -2.3445605621301056 2216 104-105 -2.355537682803199 2216 106-107 -2.319492791988708 2216 108-109 -2.299677198011736 2216 110-111 -2.3431379996042914 2216 112-113 -2.369530599022344 2216 114-115 -2.43484931306185 2216 116-117 -2.4956227329462983 2216 118-119 -2.5064822335287253 2216 120-121 -2.388322282240651 2216 122-123 -2.5431257060014367 2216 124-125 -2.754020278820878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57821.0 3 9603.0 4 5762.0 5 4541.0 6 4785.0 7 4937.0 8 5549.0 9 5968.0 10 7090.0 11 7984.0 12 8945.0 13 9784.0 14 10246.0 15 9761.0 16 9082.0 17 8998.0 18 8917.0 19 8967.0 20 9103.0 21 10300.0 22 12516.0 23 16145.0 24 19743.0 25 22708.0 26 26566.0 27 29675.0 28 35409.0 29 43599.0 30 52472.0 31 63187.0 32 81152.0 33 105732.0 34 141502.0 35 198557.0 36 346283.0 37 656117.0 38 328172.0 39 1901.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.62343620559875 18.48967422614517 11.914694095532857 23.97219547272322 2 14.983675691812198 21.170136501909933 38.82713051668065 25.019057289597225 3 20.529171841065995 26.248224869235187 28.50874722221989 24.713856067478932 4 13.162940613385205 16.759189154080314 35.43181761810333 34.646052614431156 5 13.099716474734974 38.93065907011785 34.043001331862456 13.926623123284724 6 32.41020487960748 33.58310174187608 18.528718041429997 15.477975337086447 7 27.645728957968487 32.56040564410967 21.422007588591722 18.371857809330116 8 27.014345351426627 34.52353720809758 20.90188681149377 17.560230628982016 9 27.973307809669272 16.00509520068766 20.047108318011848 35.97448867163122 10-11 24.83320250558445 27.546520251736002 26.66303867615916 20.957238566520388 12-13 25.62652135660851 25.18456042782188 26.71807106701455 22.47084714855506 14-15 24.427767370373505 25.703635013979042 24.47101106361616 25.39758655203129 16-17 23.11172572813442 28.96421497963614 26.91046397395175 21.01359531827769 18-19 23.384871508403375 27.981918372022875 27.791306650915487 20.841903468658266 20-21 22.98703177056442 27.80458353164359 27.5286239482549 21.67976074953709 22-23 22.47601630178701 28.342468728731006 25.61604257993462 23.565472389547363 24-25 22.684343282508962 28.84616920928672 25.268035727725568 23.20145178047875 26-27 23.192492941790903 27.555357636952806 25.613141178479125 23.639008242777162 28-29 22.83593907024347 27.384856871585146 26.97549505637424 22.803709001797145 30-31 23.779137982514058 28.050493921132425 26.253495716826965 21.91687237952656 32-33 21.62519328615266 28.307395736918046 27.197217268143874 22.870193708785422 34-35 21.401322418030098 28.659567346265963 27.25826174861274 22.6808484870912 36-37 23.382963184801056 27.908957647021737 26.195713737790875 22.512365430386332 38-39 24.022483329828543 27.315993680029766 26.249136319052198 22.4123866710895 40-41 22.700196472568518 28.421302054569924 26.197000668632725 22.681500804228836 42-43 23.583984672478213 27.923652303067293 25.971662151672298 22.5207008727822 44-45 24.152418772502482 27.301463276033527 25.947489515304 22.59862843615999 46-47 22.809162403854216 27.881452094773255 27.590994468484613 21.718391032887915 48-49 23.563620727231534 27.64930402236006 27.248978432059506 21.5380968183489 50-51 21.991233539245766 28.097160240807646 27.38526092293067 22.526345297015922 52-53 21.861198791198493 28.572392140184665 25.78379414233045 23.782614926286392 54-55 22.191966251903754 28.621519874663377 25.48480528780691 23.701708585625962 56-57 22.90698070167109 27.636392743199746 25.654499869114733 23.802126686014436 58-59 22.98333004527022 27.17110387106927 26.687601018648255 23.157965065012256 60-61 23.56048610095529 27.905818150032697 26.35775604852605 22.175939700485966 62-63 21.70496262018243 28.18788519471776 27.13685032881086 22.97030185628895 64-65 21.027305872297717 28.282203341566447 27.39651149423959 23.29397929189625 66-67 22.864104191269487 28.024244894871547 26.217771184339373 22.893879729519597 68-69 23.53317192260186 27.173297717065005 26.27739250923643 23.016137851096712 70-71 22.057380873322433 28.14243503040951 26.35392717689634 23.44625691937172 72-73 23.125083143648475 27.73417822341489 26.09522120256031 23.04551743037632 74-75 23.615067520620617 27.012262452549358 26.187332680131696 23.185337346698326 76-77 22.401624857887978 27.57704520475689 27.78773875344904 22.23359118390609 78-79 23.0038634020716 27.410402734345613 27.37768590074675 22.20804796283603 80-81 21.593206113270814 27.995362531786977 27.394790644620272 23.01664071032194 82-83 21.472441628257915 28.26245339832613 25.950825449320934 24.314279524095017 84-85 21.791823981443947 28.44007412838315 25.503034291837118 24.265067598335786 86-87 22.379926543816588 26.967838279552538 26.149089951566495 24.503145225064372 88-89 22.515606975283088 26.81044065146908 26.839248679894684 23.83470369335314 90-91 22.948582278608686 27.676897873964258 26.503161232082007 22.87135861534505 92-93 21.36938006260907 27.622805572274473 27.154624564140672 23.853189800975787 94-95 20.772348899017615 27.745142768039578 27.545963316768667 23.936545016174136 96-97 22.64958414127642 27.182412715886244 26.606323909723255 23.561679233114077 98-99 23.081734165911293 26.614008883034444 26.393004127326293 23.91125282372797 100-101 21.73184446713625 27.39601452182201 26.56423990829561 24.307901102746133 102-103 22.752751302419636 26.943172297361144 26.379535895546052 23.92454050467317 104-105 23.081822219282415 26.23928151080957 26.646759963588373 24.032136306319643 106-107 21.829976159857285 26.87395892337739 28.081991104524672 23.214073812240656 108-109 22.37258622989247 26.938845381135966 27.458151866270807 23.230416522700757 110-111 21.162263840185677 27.307628960926227 27.645078153426255 23.88502904546184 112-113 20.940585157381104 27.592509030958606 26.339581689526607 25.127324122133682 114-115 21.030562317398722 28.10400111587918 25.92557892534449 24.939857641377607 116-117 21.69419062101576 26.8210446999211 26.238378438429216 25.24638624063392 118-119 21.69790324482263 26.69023552111624 26.862147210627757 24.74971402343337 120-121 22.004032462309425 27.454750396552086 26.59256094144312 23.94865619969537 122-123 20.749570863191543 27.497716977473548 27.076174488255273 24.676537671079636 124-125 20.34798778337185 27.713696987877274 27.27264133642635 24.665673892324527 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9368.0 1 5165.5 2 1219.5 3 1574.0 4 1617.0 5 1457.0 6 1219.0 7 1030.5 8 978.5 9 978.0 10 932.0 11 891.0 12 920.0 13 935.0 14 986.5 15 1094.5 16 1224.5 17 1409.0 18 1630.0 19 1891.5 20 2385.5 21 3162.0 22 4078.0 23 5292.5 24 6815.5 25 8735.0 26 11525.0 27 14733.0 28 17283.0 29 20028.5 30 24039.5 31 28344.5 32 32130.0 33 36710.5 34 42649.0 35 47199.5 36 52105.5 37 56784.5 38 59005.5 39 60667.5 40 61636.5 41 64636.0 42 68502.5 43 72051.0 44 76396.5 45 79731.0 46 81147.5 47 83651.0 48 91160.0 49 109791.0 50 168500.5 51 172191.5 52 107197.0 53 79260.5 54 71472.5 55 64619.0 56 57492.5 57 50761.0 58 45679.5 59 41265.5 60 36122.5 61 29996.0 62 24885.5 63 20389.5 64 16253.0 65 12910.0 66 9340.0 67 7226.5 68 5288.5 69 3244.5 70 2453.5 71 1956.5 72 1603.5 73 1437.0 74 1086.5 75 844.5 76 613.0 77 339.0 78 202.5 79 136.0 80 103.5 81 82.0 82 66.0 83 57.5 84 50.0 85 37.5 86 31.5 87 27.5 88 27.0 89 22.0 90 15.5 91 13.5 92 11.0 93 9.5 94 8.5 95 9.0 96 8.0 97 5.5 98 3.5 99 3.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4833068921345559 2 0.46970616999898307 3 0.45535217709897857 4 0.4552266319715732 5 0.45844895690830895 6 0.4648936067817804 7 0.4496607979899388 8 0.4765274552546704 9 0.4877428199695427 10-11 0.49257630737464636 12-13 0.4924298380593402 14-15 0.46859718804023637 16-17 0.46968524581108223 18-19 0.45704703631894994 20-21 0.46665123856545443 22-23 0.4836626033288709 24-25 0.47112901477624297 26-27 0.48113077659286424 28-29 0.4756067909870316 30-31 0.48832869723076744 32-33 0.4866129138228952 34-35 0.4817166538540889 36-37 0.43800602532914795 38-39 0.39774788780785236 40-41 0.39121954118277735 42-43 0.3995055195915264 44-45 0.43509756321092546 46-47 0.4425256499157383 48-49 0.4400565957434343 50-51 0.4255770577160245 52-53 0.41982290604328215 54-55 0.4212875991963438 56-57 0.4030835557225771 58-59 0.40837737526150003 60-61 0.41810712263540983 62-63 0.41929980134576006 64-65 0.4337793393731699 66-67 0.42304523098001784 68-69 0.42442622738147595 70-71 0.4347627762045113 72-73 0.43578806141165455 74-75 0.44057970044095635 76-77 0.43078718050334386 78-79 0.42141314432374904 80-81 0.4295108050413901 82-83 0.4284645956463461 84-85 0.4265814187352668 86-87 0.4326075848507206 88-89 0.4199066027948856 90-91 0.4237985017444495 92-93 0.4340095054400796 94-95 0.43177061733468536 96-97 0.40023786616805723 98-99 0.37081845797941815 100-101 0.3718646673744622 102-103 0.3529492015120655 104-105 0.3545185156046316 106-107 0.37372692009764064 108-109 0.38067375048073326 110-111 0.3982709925053744 112-113 0.39475572893802635 114-115 0.3930817939059558 116-117 0.3646458225486582 118-119 0.4373364513163197 120-121 0.44507840083964584 122-123 0.39799897806266293 124-125 0.3925586892084338 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2389579.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.41794922456524 #Duplication Level Percentage of deduplicated Percentage of total 1 85.39923050120002 52.450456027396456 2 9.494248542834224 11.662345498583898 3 2.4737615010051206 4.558000747872503 4 0.9319889260020472 2.289633941402033 5 0.44241004878985146 1.358595895650626 6 0.27778870245885257 1.0236727453665397 7 0.1634459093847634 0.7026958794489402 8 0.13155248027787245 0.6463746843257592 9 0.08828708419162436 0.4880170488659491 >10 0.5164410163911108 6.091552113733757 >50 0.052351550518795975 2.2408661700654022 >100 0.025110923770068048 2.6331101762271474 >500 6.505409953270785E-4 0.2521952897886628 >1k 0.001691406587850404 3.0745290794102664 >5k 6.505409953270785E-4 3.388015176318037 >10k+ 3.903245971962471E-4 7.139939525544049 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 39872 1.6685784399678771 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 29487 1.2339830572665729 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 19991 0.8365908806530354 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 9930 0.4155543717115023 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 9088 0.3803180392864182 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 8719 0.36487598861556786 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 7607 0.3183405947240079 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 7054 0.2951984429056332 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 4889 0.2045967092948172 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 4312 0.1804501964572002 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 4063 0.17002995088256134 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 3807 0.1593167666773101 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 3630 0.15190960416039812 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 3558 0.14889652110267124 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 2998 0.1254614306536842 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 2772 0.11600369772248585 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 2552 0.10679705504609807 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.184837580176257E-5 0.0 0.0 0.0 0.0 3 4.184837580176257E-5 0.0 0.0 0.0 0.0 4 4.184837580176257E-5 0.0 0.0 0.0 0.0 5 4.184837580176257E-5 0.0 0.0 0.0 0.0 6 1.255451274052877E-4 0.0 0.0 0.0 4.184837580176257E-5 7 2.510902548105754E-4 0.0 0.0 0.0 4.184837580176257E-5 8 2.510902548105754E-4 0.0 0.0 4.184837580176257E-5 4.184837580176257E-5 9 2.510902548105754E-4 0.0 0.0 8.369675160352514E-5 4.184837580176257E-5 10-11 2.510902548105754E-4 0.0 0.0 1.0462093950440641E-4 4.184837580176257E-5 12-13 2.92938630612338E-4 0.0 0.0 2.510902548105754E-4 4.184837580176257E-5 14-15 3.3478700641410055E-4 0.0 0.0 8.369675160352514E-4 4.184837580176257E-5 16-17 3.3478700641410055E-4 0.0 0.0 0.001548389904665215 4.184837580176257E-5 18-19 3.975595701167444E-4 0.0 0.0 0.0023016606690969416 4.184837580176257E-5 20-21 4.603321338193883E-4 0.0 0.0 0.002824765366618974 4.184837580176257E-5 22-23 4.603321338193883E-4 0.0 0.0 0.004205761768077138 4.184837580176257E-5 24-25 6.277256370264385E-4 0.0 0.0 0.005586758169535303 4.184837580176257E-5 26-27 6.277256370264385E-4 0.0 0.0 0.0069468303830925865 4.184837580176257E-5 28-29 6.695740128282011E-4 0.0 0.0 0.009729747373909796 4.184837580176257E-5 30-31 6.695740128282011E-4 0.0 0.0 0.016048852119975945 4.184837580176257E-5 32-33 6.695740128282011E-4 0.0 0.0 0.030151754765169934 4.184837580176257E-5 34-35 7.32346576530845E-4 0.0 0.0 0.05323113401984199 4.184837580176257E-5 36-37 8.369675160352514E-4 0.0 0.0 0.08737940867408026 4.184837580176257E-5 38-39 9.206642676387766E-4 0.0 0.0 0.13826703364902354 4.184837580176257E-5 40-41 9.834368313414205E-4 0.0 0.0 0.2011232941032709 8.369675160352514E-5 42-43 0.0011299061466475894 0.0 0.0 0.25577727290037283 1.255451274052877E-4 44-45 0.001255451274052877 0.0 0.0 0.3233623998202194 1.255451274052877E-4 46-47 0.0013600722135572835 0.0 0.0 0.4090678734622291 1.255451274052877E-4 48-49 0.001548389904665215 0.0 0.0 0.495631238808175 1.255451274052877E-4 50-51 0.001548389904665215 0.0 0.0 0.5847473550780284 1.255451274052877E-4 52-53 0.001548389904665215 0.0 0.0 0.6785086410618775 1.255451274052877E-4 54-55 0.001548389904665215 0.0 0.0 0.7854730896111826 1.255451274052877E-4 56-57 0.001548389904665215 0.0 0.0 0.9147217982749263 1.255451274052877E-4 58-59 0.001548389904665215 0.0 0.0 1.0615049763996085 1.255451274052877E-4 60-61 0.001548389904665215 0.0 0.0 1.2057563277882841 1.255451274052877E-4 62-63 0.0015902382804669777 0.0 0.0 1.3473503073135475 1.255451274052877E-4 64-65 0.0015902382804669777 0.0 0.0 1.5092825974784678 1.255451274052877E-4 66-67 0.0016320866562687401 0.0 0.0 1.7080205341610384 1.255451274052877E-4 68-69 0.0016739350320705028 0.0 0.0 1.9361151064685453 1.255451274052877E-4 70-71 0.0016739350320705028 0.0 0.0 2.2162062857097418 1.255451274052877E-4 72-73 0.0017994801594757906 0.0 0.0 2.4630489303764387 1.255451274052877E-4 74-75 0.0019250252868810783 0.0 0.0 2.711293495632494 1.255451274052877E-4 76-77 0.0019250252868810783 0.0 0.0 3.0052364872640744 1.255451274052877E-4 78-79 0.0019250252868810783 0.0 0.0 3.319601486286915 1.255451274052877E-4 80-81 0.0021761155416916535 0.0 0.0 3.656962167812824 1.255451274052877E-4 82-83 0.0021761155416916535 0.0 0.0 4.027152900155215 1.255451274052877E-4 84-85 0.002197039729592535 0.0 0.0 4.395481379774429 1.255451274052877E-4 86-87 0.002217963917493416 0.0 0.0 4.781344328854581 1.255451274052877E-4 88-89 0.002217963917493416 0.0 0.0 5.211901343291014 1.255451274052877E-4 90-91 0.002364433232799585 0.0 0.0 5.668864682858361 1.255451274052877E-4 92-93 0.0024690541723039914 0.0 0.0 6.132712080245097 1.255451274052877E-4 94-95 0.0024690541723039914 0.0 0.0 6.617232575277905 1.255451274052877E-4 96-97 0.002510902548105754 0.0 0.0 7.118617965758823 1.255451274052877E-4 98-99 0.0026364476755110417 0.0 0.0 7.682064497553753 1.255451274052877E-4 100-101 0.002657371863411923 0.0 0.0 8.359003824523064 1.255451274052877E-4 102-103 0.0026992202392136858 0.0 0.0 8.997045086184638 1.255451274052877E-4 104-105 0.0029712346819251423 0.0 0.0 9.611881423464133 1.255451274052877E-4 106-107 0.00309677980933043 0.0 0.0 10.25052948657483 1.255451274052877E-4 108-109 0.0031386281851321926 0.0 0.0 10.893822719399527 1.255451274052877E-4 110-111 0.003222324936735718 0.0 0.0 11.579801295542019 1.255451274052877E-4 112-113 0.003306021688339243 0.0 0.0 12.328385041883948 1.255451274052877E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 7870 0.0 24.961578 4 TAGGCCG 135 0.0025487114 22.047995 5 ATGGGGG 3390 0.0 21.07242 5 GTACACG 460 5.456968E-12 19.410597 1 GTCCAAG 1805 0.0 18.138063 1 GTATAGG 755 0.0 18.133698 1 ATGGGGA 4540 0.0 18.094896 5 CGGTTAC 510 0.0 18.089472 28-29 ATGGGAG 2770 0.0 18.052292 5 CTCGTAA 265 2.077668E-5 17.97006 1 AGTACTC 1300 0.0 17.400955 5 GTGTAGC 1250 0.0 17.143438 1 TCTATAC 575 1.0913936E-11 16.564754 3 CATGGGT 4220 0.0 16.504646 4 ATGGGTA 2070 0.0 16.392204 5 CCAAGAC 1775 0.0 16.09814 3 ACTTGAC 1225 0.0 16.03418 8 CGCGGAA 505 0.0 15.904485 82-83 ATGGGAT 3215 0.0 15.738771 5 ATTCGCG 520 0.0 15.446188 80-81 >>END_MODULE