FastQCFastQC Report
Thu 26 May 2016
SRR1364152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364152_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2389579
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA372631.5593960275010788No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG206080.862411328522723No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC110610.46288488474329575No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG103230.432000783401595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95270.398689476263392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94220.3942953968042069No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG90600.3791462847639689No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT70900.2967049844344966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59880.25058807430095426No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC56140.23493678175109506No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT51590.2158957707612931No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA48500.20296462263854845No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC46340.19392537346536776No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG42970.17982247082017377No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG40880.1710761602776054No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37540.1570988027598167No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG33480.1401083621843011No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG31930.13362186393502787No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30810.1289348458452305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT123700.022.43825312-13
GTTATCA14250.021.321471
CATGGGG69350.021.019274
GTACTTT137200.020.31607814-15
AGTACTT128950.019.72522212-13
GTATAGT6150.018.4051911
CATGGGT40850.017.9147624
ACTTTTT159050.017.84249116-17
CCAAGAC17400.017.7808343
ATGGGGA36800.017.6228715
GTGTAAG9450.017.0213431
AGTACTC12950.016.999265
GTATAGG7400.016.101271
ATGGGTA21450.016.0878775
TACTTTT158450.016.05196614-15
ATGGGAG23850.015.7162955
TTATCAA19350.015.6814872
TAGACAG13350.015.1528975
GGGTACC8750.014.9596617
TATACAG12500.014.7553575
Produced by FastQC (version 0.11.5)