##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364151_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1207253 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.835471106719137 31.0 30.0 34.0 25.0 34.0 2 29.858898673268982 31.0 30.0 34.0 25.0 34.0 3 30.03035900511326 31.0 31.0 34.0 25.0 34.0 4 33.18086184089002 37.0 35.0 37.0 26.0 37.0 5 33.333621867164545 37.0 35.0 37.0 28.0 37.0 6 33.106676065414625 37.0 35.0 37.0 26.0 37.0 7 33.031893066324955 37.0 35.0 37.0 25.0 37.0 8 32.87092017994571 37.0 35.0 37.0 23.0 37.0 9 34.150965870451344 39.0 35.0 39.0 19.0 39.0 10-11 34.18287467498528 39.0 35.0 39.0 21.0 39.0 12-13 34.083242286413864 39.0 35.0 39.0 19.0 39.0 14-15 35.1974383165749 40.0 36.0 41.0 17.0 41.0 16-17 35.25748703875658 40.0 36.0 41.0 19.0 41.0 18-19 35.185765535475994 40.0 35.5 41.0 19.0 41.0 20-21 35.184595938051096 40.0 35.0 41.0 20.5 41.0 22-23 35.18371915414582 40.0 35.0 41.0 22.0 41.0 24-25 35.163342315156804 40.0 35.0 41.0 22.5 41.0 26-27 34.969320432419714 40.0 35.0 41.0 20.5 41.0 28-29 34.853188602554724 40.0 35.0 41.0 20.0 41.0 30-31 34.90945932625556 40.0 35.0 41.0 23.0 41.0 32-33 34.61345260686865 39.0 34.5 41.0 21.0 41.0 34-35 34.66828908273577 39.0 35.0 41.0 22.0 41.0 36-37 34.65469002769096 39.5 34.5 41.0 21.5 41.0 38-39 34.522762420138946 39.5 34.0 41.0 20.0 41.0 40-41 34.20977790073829 39.0 33.0 41.0 17.5 41.0 42-43 33.9913518541681 39.0 33.0 41.0 15.5 41.0 44-45 33.75807225163243 38.0 33.0 41.0 14.5 41.0 46-47 33.51536132028664 38.0 33.0 40.0 10.5 41.0 48-49 33.24388591289481 38.0 33.0 40.0 8.5 41.0 50-51 33.137417757504025 38.0 32.5 40.0 7.5 41.0 52-53 32.969333271484935 38.0 32.0 40.0 4.0 41.0 54-55 32.68152615897414 38.0 32.0 40.0 2.0 41.0 56-57 32.32331996690006 37.5 31.0 40.0 2.0 41.0 58-59 31.921478762115314 37.0 31.0 40.0 2.0 41.0 60-61 31.61423496152008 36.0 30.0 40.0 2.0 41.0 62-63 31.08972974181882 36.0 29.5 39.0 2.0 41.0 64-65 30.83160364894517 35.5 29.0 39.0 2.0 41.0 66-67 30.39076357648314 35.0 29.0 38.5 2.0 40.5 68-69 30.025900536175932 35.0 29.0 38.0 2.0 40.0 70-71 29.47294270546439 35.0 28.0 37.0 2.0 40.0 72-73 29.274184450152536 34.0 28.0 37.0 2.0 39.0 74-75 28.971042937975717 34.0 28.0 36.0 2.0 39.0 76-77 28.32484574484387 34.0 26.0 36.0 2.0 38.5 78-79 27.902731241918637 34.0 26.0 35.0 2.0 37.0 80-81 27.761967044190406 34.0 26.0 35.0 2.0 37.0 82-83 27.58497804519848 34.0 26.0 35.0 2.0 36.5 84-85 27.33403685888542 34.0 26.0 35.0 2.0 36.0 86-87 27.031509965185425 34.0 25.5 35.0 2.0 36.0 88-89 26.698637319600778 33.0 25.0 35.0 2.0 35.5 90-91 26.53440496731008 33.0 24.5 35.0 2.0 35.0 92-93 26.226652988230306 33.0 23.5 35.0 2.0 35.0 94-95 26.13602492600971 33.0 24.0 35.0 2.0 35.0 96-97 26.04875448642497 33.0 23.5 35.0 2.0 35.0 98-99 25.87698228954494 33.0 22.5 35.0 2.0 35.0 100-101 25.37761678786468 33.0 18.5 35.0 2.0 35.0 102-103 24.20127181295056 30.5 17.0 34.0 2.0 35.0 104-105 24.941404577168164 32.0 18.0 35.0 2.0 35.0 106-107 24.821590006402964 33.0 13.5 35.0 2.0 35.0 108-109 24.71488329289718 33.0 10.0 35.0 2.0 35.0 110-111 24.81680062091376 33.0 10.0 35.0 2.0 35.0 112-113 24.729038569380236 33.0 7.5 35.0 2.0 35.0 114-115 24.56228396201956 33.0 4.5 35.0 2.0 35.0 116-117 24.22976045617613 33.0 2.0 35.0 2.0 35.0 118-119 23.93071957576415 32.5 2.0 35.0 2.0 35.0 120-121 23.704075699128516 32.5 2.0 35.0 2.0 35.0 122-123 23.234795026394632 32.0 2.0 35.0 2.0 35.0 124-125 22.196774826817574 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4617508640491401 1101 2 0.528462500490118 1101 3 0.5340181008630189 1101 4 0.5740877268948168 1101 5 0.5276170607922523 1101 6 0.5596557294136062 1101 7 0.4842013197874522 1101 8 0.6168247860316143 1101 9 0.7639063710289733 1101 10-11 0.7598064004049405 1101 12-13 0.6834199825078002 1101 14-15 0.6255451256351208 1101 16-17 0.6906761068938181 1101 18-19 0.7610558038948696 1101 20-21 0.7860711306704005 1101 22-23 0.6428601910063918 1101 24-25 0.769499574877166 1101 26-27 0.8578171352436641 1101 28-29 0.7914526829071278 1101 30-31 0.8378883895235774 1101 32-33 0.939376819321545 1101 34-35 0.8980070926887862 1101 36-37 0.971787106116544 1101 38-39 0.9474715910427634 1101 40-41 0.9160153015558308 1101 42-43 1.0081095660174952 1101 44-45 1.020394047728871 1101 46-47 1.0070139696729044 1101 48-49 1.0423208402372452 1101 50-51 0.9587861815375831 1101 52-53 1.0383488489845902 1101 54-55 0.9840005453770573 1101 56-57 0.8661224466037467 1101 58-59 0.7175635567612133 1101 60-61 0.9481196414161168 1101 62-63 1.0238278959324312 1101 64-65 0.7817753566591712 1101 66-67 0.7715559145503299 1101 68-69 0.952450717669219 1101 70-71 0.8361036224631562 1101 72-73 0.7495855419601156 1101 74-75 0.7639482630531589 1101 76-77 0.7236206933824008 1101 78-79 0.5900681689260949 1101 80-81 0.6385952251428044 1101 82-83 0.8016984868591948 1101 84-85 0.841227054200953 1101 86-87 0.6379105947853567 1101 88-89 0.5990769274015513 1101 90-91 0.7283776412405203 1101 92-93 0.7709979029258491 1101 94-95 0.7528989930109802 1101 96-97 0.6550717623096958 1101 98-99 0.888612188024787 1101 100-101 0.9438140402199977 1101 102-103 0.7703379835436905 1101 104-105 0.9090607753951829 1101 106-107 0.9207051473193957 1101 108-109 0.8144897337006896 1101 110-111 0.7857415820868638 1101 112-113 0.9697292143122631 1101 114-115 1.1085021806761937 1101 116-117 0.8924920707749031 1101 118-119 0.9114131180370997 1101 120-121 1.0381310371644688 1101 122-123 1.0480080874654796 1101 124-125 0.9314408008455537 1102 1 0.4538098544064866 1102 2 0.5176854159750874 1102 3 0.5016868473179237 1102 4 0.612091130184659 1102 5 0.4981218821195341 1102 6 0.5556852245922812 1102 7 0.5312801626689065 1102 8 0.5334441847837397 1102 9 0.7514276415905172 1102 10-11 0.6767094066442993 1102 12-13 0.6797330851737087 1102 14-15 0.7801112061796545 1102 16-17 0.7196040705920623 1102 18-19 0.9836876813764377 1102 20-21 1.0362129344140172 1102 22-23 0.9613513991742835 1102 24-25 0.8809573173615632 1102 26-27 0.9196435674614705 1102 28-29 1.0274141122888594 1102 30-31 0.999828264736287 1102 32-33 1.101033087047007 1102 34-35 1.1586423734601965 1102 36-37 1.0409873330025263 1102 38-39 0.9471879835555299 1102 40-41 0.9846483134673463 1102 42-43 1.1879167129261745 1102 44-45 1.0935647794361856 1102 46-47 1.0257320638305885 1102 48-49 1.020199456232703 1102 50-51 1.1315031412653198 1102 52-53 1.0774866822233875 1102 54-55 1.0560368471354238 1102 56-57 1.127892157324112 1102 58-59 1.30775073770279 1102 60-61 1.271999391841522 1102 62-63 1.1574070224213706 1102 64-65 1.3574985557516293 1102 66-67 1.3532348708747755 1102 68-69 1.2751960381456833 1102 70-71 1.2657689656282187 1102 72-73 1.2149854285171209 1102 74-75 1.223108784890936 1102 76-77 1.2085894965588047 1102 78-79 1.2035111638211582 1102 80-81 1.2075118445415605 1102 82-83 1.1513865186232337 1102 84-85 1.1744658517052073 1102 86-87 1.0845923871846779 1102 88-89 1.1047490771463018 1102 90-91 1.0369425873550995 1102 92-93 1.0123478745650978 1102 94-95 1.139455998115917 1102 96-97 1.271067224589899 1102 98-99 1.1296785521767987 1102 100-101 0.8306546528121714 1102 102-103 0.6404457543888427 1102 104-105 0.775198041419003 1102 106-107 0.8844033889529719 1102 108-109 0.9582787297301856 1102 110-111 0.808997396040354 1102 112-113 0.7859515625822553 1102 114-115 0.7670387660420452 1102 116-117 0.6054812936903886 1102 118-119 0.75202571020953 1102 120-121 0.5080686435172765 1102 122-123 0.5440375826441546 1102 124-125 0.48078850362490755 1103 1 0.37707980901339866 1103 2 0.2914333723329001 1103 3 0.4270137109051042 1103 4 0.378375999208842 1103 5 0.3324884364553924 1103 6 0.3773944641587832 1103 7 0.4613554258995549 1103 8 0.2960934759239109 1103 9 0.3768604806365161 1103 10-11 0.35416523299072367 1103 12-13 0.4526418175832845 1103 14-15 0.5560814694976841 1103 16-17 0.4948388610482297 1103 18-19 0.428236777248479 1103 20-21 0.42274134044460965 1103 22-23 0.43824573036108916 1103 24-25 0.550402542984429 1103 26-27 0.6321073618277708 1103 28-29 0.7232848826049292 1103 30-31 0.5868407895420162 1103 32-33 0.6966164366659271 1103 34-35 0.5356777934137753 1103 36-37 0.5249143354541062 1103 38-39 0.45578804297804965 1103 40-41 0.6071608692553951 1103 42-43 0.7203205763433544 1103 44-45 0.8227579201070867 1103 46-47 0.5868862589175876 1103 48-49 0.5988761452860913 1103 50-51 0.5369971823304098 1103 52-53 0.7238338654528889 1103 54-55 0.7113833646420247 1103 56-57 0.7776267420258378 1103 58-59 0.7981457385845943 1103 60-61 0.7072471154346509 1103 62-63 0.6602218068717427 1103 64-65 0.5915586233407701 1103 66-67 0.711865356534723 1103 68-69 0.5387651072934752 1103 70-71 0.8416929937760571 1103 72-73 0.762182004254619 1103 74-75 0.7487654911355435 1103 76-77 0.610107789638942 1103 78-79 0.7026991089935599 1103 80-81 0.6407268094838621 1103 82-83 0.7665851789154843 1103 84-85 0.7996598884804378 1103 86-87 0.7287031228005354 1103 88-89 0.7980724643571158 1103 90-91 0.8331602008894521 1103 92-93 0.7568984003908561 1103 94-95 0.762257086787347 1103 96-97 0.8308405610373555 1103 98-99 0.8840077370555157 1103 100-101 0.9361682365780624 1103 102-103 0.8286476829197582 1103 104-105 0.8031415614248516 1103 106-107 0.8376618562650258 1103 108-109 0.8645443102675614 1103 110-111 0.7825028958012368 1103 112-113 0.8077882269133347 1103 114-115 0.8739098525367694 1103 116-117 0.837310315713264 1103 118-119 0.7722269841394116 1103 120-121 0.7682280674683639 1103 122-123 0.6868983798274648 1103 124-125 0.7239209920144773 1104 1 0.14401155655870213 1104 2 0.16582918991090878 1104 3 0.1397380628034952 1104 4 0.12830843724834295 1104 5 -0.03907635491671613 1104 6 0.10514215350841738 1104 7 -0.028331784926642456 1104 8 0.025658057485991037 1104 9 0.13021564047574685 1104 10-11 -0.029456432556436596 1104 12-13 0.028803014314632946 1104 14-15 0.06017573996160053 1104 16-17 0.11494038404983087 1104 18-19 -0.14084802827797915 1104 20-21 -0.03816118713992722 1104 22-23 -0.011496156332093221 1104 24-25 0.00453963661450274 1104 26-27 0.18122811689335094 1104 28-29 0.08461626814964518 1104 30-31 0.06520890593333917 1104 32-33 0.19664321093069503 1104 34-35 0.1561070271531051 1104 36-37 0.23985547121528583 1104 38-39 0.18348374954462798 1104 40-41 0.3094820884082381 1104 42-43 0.2213498980776336 1104 44-45 0.15779196726423805 1104 46-47 0.22932894868214504 1104 48-49 0.34863040137645385 1104 50-51 0.3494506600881948 1104 52-53 0.23859681926493437 1104 54-55 0.3849822172630084 1104 56-57 0.3099182276530641 1104 58-59 0.2371229616705861 1104 60-61 0.28015140291823215 1104 62-63 0.44038974883891413 1104 64-65 0.4624512397501057 1104 66-67 0.39343104038232823 1104 68-69 0.4224403213701642 1104 70-71 0.44366505146442137 1104 72-73 0.4391429842866543 1104 74-75 0.2975975225506815 1104 76-77 0.36722215019591076 1104 78-79 0.4197394410570716 1104 80-81 0.608383497648223 1104 82-83 0.37579756146492826 1104 84-85 0.49123131861761493 1104 86-87 0.47031267886443473 1104 88-89 0.25295626248229297 1104 90-91 0.15642171343186817 1104 92-93 0.15429080620729252 1104 94-95 0.22406899992599705 1104 96-97 0.1993430654488435 1104 98-99 0.19211611176369914 1104 100-101 0.23379722668724412 1104 102-103 0.1406096728582682 1104 104-105 0.24292655311856493 1104 106-107 0.29244560319029134 1104 108-109 0.24235783925657728 1104 110-111 0.27541820614001367 1104 112-113 0.1957396511946179 1104 114-115 0.29340918944313543 1104 116-117 0.12266166139407275 1104 118-119 0.22104194891794293 1104 120-121 0.15611983680885544 1104 122-123 0.18889173166791906 1104 124-125 0.42818874718809496 1105 1 0.1986571096584271 1105 2 0.23594902262971829 1105 3 0.2795626662670685 1105 4 0.2919662518236521 1105 5 0.07444742188442888 1105 6 0.028259534957740584 1105 7 0.07489717287356967 1105 8 0.18387551534517854 1105 9 0.19683645267670613 1105 10-11 0.19795329867572065 1105 12-13 0.29010632173357465 1105 14-15 0.27399852163964056 1105 16-17 0.20709135378844934 1105 18-19 0.07678915691594312 1105 20-21 -0.01923980981365503 1105 22-23 0.009936906831683245 1105 24-25 0.17153188921833618 1105 26-27 0.20858983555120858 1105 28-29 0.22352917757061874 1105 30-31 0.18893038684882413 1105 32-33 -0.003851433968563356 1105 34-35 0.1660012071829513 1105 36-37 0.22413776335737623 1105 38-39 0.2803648232314302 1105 40-41 0.4723330160957886 1105 42-43 0.4883108687906059 1105 44-45 0.3937997313269861 1105 46-47 0.2900787974008736 1105 48-49 0.2639098422511168 1105 50-51 0.40624171500586215 1105 52-53 0.4099938569724273 1105 54-55 0.45266744708234086 1105 56-57 0.44046387229078476 1105 58-59 0.46442436012495847 1105 60-61 0.38421721540123954 1105 62-63 0.40942788838691513 1105 64-65 0.6028684683797039 1105 66-67 0.5670601179255179 1105 68-69 0.4505779975872066 1105 70-71 0.3454847857221992 1105 72-73 0.39221197495849935 1105 74-75 0.5183640186199341 1105 76-77 0.2647693102104718 1105 78-79 0.23872386293183112 1105 80-81 0.34977921484967567 1105 82-83 0.26577969617192565 1105 84-85 0.23112880632280763 1105 86-87 0.29115734760972245 1105 88-89 0.2540254877683168 1105 90-91 0.18738774052916085 1105 92-93 0.04770465408499547 1105 94-95 0.2884265224515197 1105 96-97 0.22988153063620587 1105 98-99 0.37039805006145343 1105 100-101 0.34173176933061455 1105 102-103 0.2988885086078312 1105 104-105 0.27077496799849143 1105 106-107 0.23997936583766233 1105 108-109 0.2524823065669999 1105 110-111 0.38423094940799984 1105 112-113 0.42686090185486947 1105 114-115 0.22721305455151963 1105 116-117 -0.0023163835903083907 1105 118-119 0.20168099051260313 1105 120-121 0.13871640041622513 1105 122-123 0.006750678545113686 1105 124-125 0.1692570622223748 1106 1 -0.09079527490446537 1106 2 -0.1594173811916484 1106 3 -0.14682706507470655 1106 4 -0.23307386999503876 1106 5 -0.10137965623622591 1106 6 -0.20907681856918714 1106 7 -0.1465664205249695 1106 8 -0.13149177305171378 1106 9 -0.07485144380268594 1106 10-11 -0.006575096198794483 1106 12-13 -0.07097093330219195 1106 14-15 -0.02921612061274459 1106 16-17 0.08352326042781755 1106 18-19 0.1880464862560487 1106 20-21 0.13477458349390048 1106 22-23 0.10110426220358448 1106 24-25 0.10254273790537383 1106 26-27 0.17195735141038426 1106 28-29 0.3142842575615248 1106 30-31 0.14744916512365336 1106 32-33 -0.22205615633821907 1106 34-35 0.11617875412863299 1106 36-37 0.09087724954536469 1106 38-39 0.0485467993731703 1106 40-41 0.05365946869517302 1106 42-43 0.03619436387489117 1106 44-45 -0.04235539794740362 1106 46-47 0.04362168035888914 1106 48-49 0.08528260869790927 1106 50-51 0.0796112527519739 1106 52-53 0.13723988901934092 1106 54-55 0.21001289910043397 1106 56-57 0.05973198081556319 1106 58-59 0.03408341944206228 1106 60-61 0.09082467869135868 1106 62-63 0.12187655141418574 1106 64-65 0.19883227660372782 1106 66-67 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-2.461894326043648 2216 96-97 -2.522685381061539 2216 98-99 -2.5771997066470433 2216 100-101 -2.457250399758646 2216 102-103 -2.2219349670586652 2216 104-105 -2.411954869896924 2216 106-107 -2.2413712071113814 2216 108-109 -2.2975974044243586 2216 110-111 -2.4258310722270657 2216 112-113 -2.571366697896469 2216 114-115 -2.648570196884812 2216 116-117 -2.5094125426143563 2216 118-119 -2.5815985339832466 2216 120-121 -2.596552285760538 2216 122-123 -2.649833533481665 2216 124-125 -2.6921960288119813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57461.0 3 8942.0 4 6651.0 5 4985.0 6 5524.0 7 4549.0 8 4750.0 9 5250.0 10 6364.0 11 7336.0 12 7903.0 13 8562.0 14 9179.0 15 8887.0 16 8888.0 17 9057.0 18 9270.0 19 9786.0 20 9876.0 21 10753.0 22 12465.0 23 16460.0 24 18929.0 25 18146.0 26 21284.0 27 22438.0 28 25616.0 29 31859.0 30 36661.0 31 44708.0 32 56377.0 33 68604.0 34 83782.0 35 102989.0 36 141404.0 37 213025.0 38 88104.0 39 429.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.9782639456507 17.167104761555965 9.470507252287721 22.384124040505604 2 13.180052648968868 24.340202556213136 36.504228313094075 25.975516481723915 3 22.16768814050154 33.76780297829316 25.69129438002155 18.373214501183742 4 13.422889720487873 19.802013978518783 31.698983357757044 35.076112943236296 5 11.172099750266502 43.40627837171429 31.181819467895387 14.239802410123817 6 29.56015669277381 30.632738203725573 21.27869226281576 18.52841284068486 7 25.18422611717247 35.149411208669775 21.28860094659273 18.37776172756502 8 29.175881295877197 33.630365536052125 21.292930739004245 15.90082242906643 9 31.925348472299103 18.118561742756896 18.221448995475793 31.73464078946821 10-11 23.074790710296767 32.3708622282085 22.98867319505971 21.565673866435024 12-13 26.493898289423246 29.53482651362755 22.625256865386927 21.346018331562277 14-15 27.505796756479633 27.774162029538907 22.114896457362004 22.605144756619456 16-17 22.6265753646981 31.013039868664986 27.947141403611713 18.4132433630252 18-19 24.69365371156472 29.823919018892987 27.443176664339337 18.039250605202955 20-21 21.735671175234945 30.663618417329413 27.727112330262894 19.87359807717275 22-23 20.48436166892103 32.70069711788576 21.812464883987097 25.002476329206118 24-25 20.905689899834794 34.06923758754573 21.016008955360522 24.009063557258962 26-27 23.329009231271208 29.005776983707833 22.17906443744431 25.486149347576653 28-29 23.031164113909405 29.21146684911349 25.150568564879322 22.606800472097788 30-31 25.687665964655253 30.968775753136157 23.660046116323013 19.683512165885574 32-33 19.40920701103648 31.47424801335515 26.441802845086027 22.67474213052235 34-35 17.385579078839324 32.69505691965075 27.214347755697272 22.70501624581265 36-37 23.767661589713637 30.224219611915114 24.425077918166373 21.583040880204877 38-39 26.430536293868993 28.38429854308877 23.585910356399534 21.5992548066427 40-41 21.509151361726616 32.50862410224509 23.660519741459893 22.321704794568394 42-43 24.75219197200131 30.4860430726033 22.991128979940815 21.77063597545457 44-45 26.442189494807806 28.364443235296687 23.449548795143883 21.743818474751624 46-47 22.39465395526353 29.85982982217986 28.884966266883723 18.86054995567289 48-49 24.429238656223596 29.24980540926872 27.388889836477226 18.93206609803046 50-51 20.543622670328197 30.664857530529172 28.035301448279476 20.756218350863154 52-53 19.594946733399134 32.41703050502303 22.566463150110202 25.421559611467636 54-55 20.51323645263236 33.37763092329487 21.61788940493155 24.491243219141218 56-57 23.090214820162554 28.764176899762788 22.471737691173978 25.673870588900684 58-59 22.659060842022345 28.71664601765127 25.3925912247715 23.231701915554883 60-61 24.967162535260243 30.546412085850733 24.04401176908046 20.442413609808565 62-63 19.158970826725163 31.11673178131295 26.535996482852447 23.188300909109437 64-65 17.233878636661256 32.042928181530016 27.32064517900331 23.402548002805414 66-67 23.14874703067879 29.89997791336632 24.623560157376623 22.32771489857826 68-69 25.42159333204109 28.31669643533073 23.944737601774307 22.31697263085387 70-71 20.982057799428414 31.63930061610014 24.033063902189422 23.345577682282027 72-73 23.998124688262703 29.95831300150965 23.35478626729349 22.688776042934155 74-75 25.624418098660513 27.956974038151927 23.66710792018165 22.75149994300591 76-77 21.798901918502036 29.264217177042866 28.841340576228646 20.095540328226452 78-79 23.69956309645819 28.968776204229947 27.45492040569139 19.876740293620475 80-81 20.054318910826385 30.28909491083637 27.809967840442674 21.846618337894576 82-83 19.15856711322015 31.639713449265862 22.899475133961886 26.3022443035521 84-85 20.029499739818284 32.69200932245021 22.048476574702516 25.230014363028992 86-87 22.21806699110748 28.15380308336637 23.126703719865503 26.501426205660643 88-89 22.019719698277385 28.04909751496863 25.670629878192074 24.26055290856191 90-91 24.032291407994734 29.799671977994326 24.383981245427098 21.784055368583843 92-93 18.867659238669457 30.292567607198734 26.414822770945655 24.42495038318615 94-95 17.17436048465729 30.94295027304402 27.415216588297543 24.467472654001153 96-97 22.531923086839612 28.992350768457918 25.212979989903044 23.262746154799423 98-99 24.482177734578 27.736240948347902 24.235271031969408 23.546310285104695 100-101 20.525732882230812 30.413209465835966 24.443515040954267 24.617542610978955 102-103 23.027024870096323 29.004693200366944 24.17058459461448 23.79769733492226 104-105 24.285800981971693 27.305289113016528 24.406926342147138 24.00198356286464 106-107 20.87872341398539 28.655627677101865 28.807247834309557 21.658401074603187 108-109 22.48258204431938 28.730344642063454 27.43711054648537 21.349962767131796 110-111 19.177233403029714 29.895255822309164 27.95186624948642 22.975644525174697 112-113 18.33681588626294 31.226419997962445 23.444629487555332 26.992134628219283 114-115 19.096584038442664 32.69913690293027 22.661080127144228 25.543198931482834 116-117 21.026686908964535 28.577848319118825 23.501615448442834 26.893849323473805 118-119 20.687936626928146 28.5331914466987 25.58173589533559 25.197136031037566 120-121 22.23613177435406 30.16936093165986 24.744427581019327 22.850079712966753 122-123 18.239417408545915 30.611335116504986 26.150619785220968 24.998627689728135 124-125 16.934887631619 31.482648871575787 26.752639864806987 24.82982363199823 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9462.0 1 6309.5 2 5557.0 3 7630.0 4 6398.5 5 4792.0 6 3590.0 7 2958.5 8 2811.0 9 2594.0 10 2110.5 11 1730.0 12 1620.0 13 1535.5 14 1537.0 15 1541.0 16 1535.5 17 1646.5 18 1730.5 19 1796.5 20 1937.5 21 2255.0 22 2657.0 23 3020.0 24 3456.0 25 4074.5 26 4856.0 27 5682.0 28 6422.5 29 7466.0 30 8955.0 31 10407.0 32 11753.0 33 13478.5 34 15627.0 35 17150.5 36 19098.0 37 21330.5 38 22432.0 39 22725.0 40 22170.0 41 21643.5 42 21160.5 43 21762.0 44 23462.5 45 24875.0 46 27036.5 47 32407.0 48 41078.5 49 68053.5 50 159277.0 51 172349.0 52 81919.5 53 39528.0 54 27985.5 55 22690.5 56 19534.5 57 17052.5 58 16038.5 59 15200.0 60 13209.5 61 11073.0 62 9237.5 63 6917.0 64 4947.5 65 3850.0 66 2849.0 67 2234.5 68 1768.0 69 1251.0 70 954.0 71 797.0 72 786.0 73 754.0 74 536.0 75 418.5 76 357.5 77 255.0 78 190.0 79 135.0 80 99.5 81 78.5 82 64.0 83 50.5 84 41.5 85 39.0 86 40.5 87 35.0 88 24.0 89 19.5 90 15.5 91 14.0 92 12.0 93 10.0 94 9.5 95 6.5 96 2.0 97 2.0 98 3.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4836600116131416 2 0.47297459604573355 3 0.46038402886553187 4 0.45988703279262927 5 0.4615436863689716 6 0.46932995817778045 7 0.45301192045080857 8 0.4814235292850794 9 0.491860446816036 10-11 0.4959606644174833 12-13 0.4962919951327518 14-15 0.4737200901550876 16-17 0.4759151561437412 18-19 0.46303467458767966 20-21 0.47123510979057415 22-23 0.4868904860870091 24-25 0.47529391105261287 26-27 0.484364089383087 28-29 0.48100936589099386 30-31 0.4922746102101217 32-33 0.49024520957910234 34-35 0.4859379102806123 36-37 0.4432376643503888 38-39 0.4042648889669357 40-41 0.3983423524315119 42-43 0.4067912856708577 44-45 0.43938594478539295 46-47 0.44667522052129915 48-49 0.44493573426613975 50-51 0.43122692592190703 52-53 0.4257185527805688 54-55 0.4270852919810512 56-57 0.4096075967506396 58-59 0.4155301332860635 60-61 0.42410331554363506 62-63 0.42596705081702013 64-65 0.438350536300179 66-67 0.42812070046626516 68-69 0.43122692592190703 70-71 0.4421608395257664 72-73 0.4406284349676497 74-75 0.44514281596318256 76-77 0.4346644820928174 78-79 0.42716812465986825 80-81 0.43714946245733083 82-83 0.433380575571152 84-85 0.4314340076189498 86-87 0.437977789245502 88-89 0.42704387564164265 90-91 0.43068851350959575 92-93 0.4383091199607704 94-95 0.434788731111043 96-97 0.40670845299204067 98-99 0.3787110075518553 100-101 0.3785453421942211 102-103 0.3597009077633272 104-105 0.3617303083943465 106-107 0.3790837546065324 108-109 0.38819534927641514 110-111 0.40546596280978386 112-113 0.4001232550260798 114-115 0.40082733279602534 116-117 0.3720015605676689 118-119 0.44352757872624876 120-121 0.4506926054439293 122-123 0.4059629588826865 124-125 0.39958484261376864 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1207253.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.445110989389164 #Duplication Level Percentage of deduplicated Percentage of total 1 86.70333987940333 41.136495837290234 2 8.549607145159127 8.112741198354989 3 2.0866388464932806 2.97002434999934 4 0.8317166762624536 1.578435600679919 5 0.43322420160647496 1.0277185164254357 6 0.28502769865248956 0.8113902478570523 7 0.1860919333072166 0.6180406700993628 8 0.14138856407580033 0.5366556892165366 9 0.10309352436162436 0.4402155335062109 >10 0.6022969854949499 5.358932517495411 >50 0.04724511560910156 1.5412189067071234 >100 0.020025200302150457 1.7998358504403116 >500 0.002916291303413045 0.915691393909848 >1k 0.003305130143868117 5.033950482506511 >5k 0.002333033042730436 7.259301029305654 >10k+ 0.0017497747820478266 20.859352176206045 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 62816 5.203217552575972 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 43819 3.6296451530872154 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 29559 2.4484511531551383 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 17396 1.4409572807025535 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 16181 1.3403155759397574 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 16178 1.340067077903306 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 15436 1.2786052302210058 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 12972 1.0745055096156315 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 11779 0.9756861237868117 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 9971 0.8259246404854658 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 9144 0.7574220151037107 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 6887 0.5704686590134794 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 6698 0.5548132827170444 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6604 0.5470270109082355 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 6464 0.5354304358738392 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 6191 0.5128171145567665 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 5739 0.4753767437314299 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 5730 0.47463124962207587 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5142 0.4259256344776116 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 5088 0.42145266982148727 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 5040 0.4174767012382657 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 4962 0.41101575229053067 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 4601 0.3811131552375517 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4500 0.37274705467702296 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4002 0.3314963806260991 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 3959 0.3279345754369631 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 3946 0.32685775061234057 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 3917 0.3244556029266442 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 3823 0.3166693311178353 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 3723 0.30838606323612366 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 3078 0.2549589853990837 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 2801 0.23201433336674251 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2442 0.2022774016713978 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2396 0.19846709844581045 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 2275 0.1884443443089394 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 1719 0.1423893748866228 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1355 0.11223827979719248 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.283267881711621E-5 0.0 12-13 0.0 0.0 0.0 5.798287517198135E-4 0.0 14-15 8.283267881711621E-5 0.0 0.0 0.0012839065216653013 0.0 16-17 8.283267881711621E-5 0.0 0.0 0.0029405600980076255 0.0 18-19 1.2424901822567433E-4 0.0 0.0 0.0040588012620386945 0.0 20-21 1.6566535763423243E-4 0.0 0.0 0.006005369214240925 0.0 22-23 1.6566535763423243E-4 0.0 0.0 0.010395501191548084 0.0 24-25 2.4849803645134866E-4 0.0 0.0 0.014247220756543989 0.0 26-27 2.4849803645134866E-4 0.0 0.0 0.018264605679174123 0.0 28-29 2.4849803645134866E-4 0.0 0.0 0.027044869633788444 8.283267881711621E-5 30-31 2.4849803645134866E-4 0.0 0.0 0.04071226163861262 8.283267881711621E-5 32-33 2.4849803645134866E-4 0.0 0.0 0.06730155153890692 8.283267881711621E-5 34-35 2.8991437585990673E-4 0.0 0.0 0.10329235048494392 1.6566535763423243E-4 36-37 4.1416339408558106E-4 0.0 0.0 0.15448294599392176 1.6566535763423243E-4 38-39 4.1416339408558106E-4 0.0 0.0 0.23346390524604205 1.6566535763423243E-4 40-41 4.1416339408558106E-4 0.0 0.0 0.3594524097268758 1.6566535763423243E-4 42-43 4.1416339408558106E-4 0.0 0.0 0.44978144597694103 1.6566535763423243E-4 44-45 4.969960729026973E-4 0.0 0.0 0.5401933149058233 1.6566535763423243E-4 46-47 5.384124123112554E-4 0.0 0.0 0.6646908311679491 1.6566535763423243E-4 48-49 6.626614305369297E-4 0.0 0.0 0.7765563639104645 1.6566535763423243E-4 50-51 6.626614305369297E-4 0.0 0.0 0.8939716861337268 1.6566535763423243E-4 52-53 6.626614305369297E-4 0.0 0.0 1.0484960484670571 1.6566535763423243E-4 54-55 6.626614305369297E-4 0.0 0.0 1.198837360520123 1.6566535763423243E-4 56-57 6.626614305369297E-4 0.0 0.0 1.3561780339332352 1.6566535763423243E-4 58-59 6.626614305369297E-4 0.0 0.0 1.5512904088869524 1.6566535763423243E-4 60-61 6.626614305369297E-4 0.0 0.0 1.7408944106993314 1.6566535763423243E-4 62-63 6.626614305369297E-4 0.0 0.0 1.923499051151664 1.6566535763423243E-4 64-65 6.626614305369297E-4 0.0 0.0 2.130166584800369 1.6566535763423243E-4 66-67 7.454941093540459E-4 0.0 0.0 2.355927050916419 1.6566535763423243E-4 68-69 7.454941093540459E-4 0.0 0.0 2.6404573026532137 1.6566535763423243E-4 70-71 8.283267881711621E-4 0.0 0.0 3.07429345795786 1.6566535763423243E-4 72-73 8.283267881711621E-4 0.0 0.0 3.4092273947548692 1.6566535763423243E-4 74-75 8.283267881711621E-4 0.0 0.0 3.7184832011185724 1.6566535763423243E-4 76-77 8.283267881711621E-4 0.0 0.0 4.080959003622273 1.6566535763423243E-4 78-79 8.283267881711621E-4 0.0 0.0 4.433494884667919 1.6566535763423243E-4 80-81 0.0010768248246225108 0.0 0.0 4.805579277914406 1.6566535763423243E-4 82-83 0.0012424901822567433 0.0 0.0 5.268986699556763 1.6566535763423243E-4 84-85 0.0012839065216653013 0.0 0.0 5.714916425968708 1.6566535763423243E-4 86-87 0.0013253228610738594 0.0 0.0 6.171655816966286 1.6566535763423243E-4 88-89 0.0013253228610738594 0.0 0.0 6.687661989657512 1.6566535763423243E-4 90-91 0.0013253228610738594 0.0 0.0 7.17459389208393 1.6566535763423243E-4 92-93 0.0013253228610738594 0.0 0.0 7.66086313307981 1.6566535763423243E-4 94-95 0.0013253228610738594 0.0 0.0 8.22039787848943 1.6566535763423243E-4 96-97 0.0013253228610738594 0.0 0.0 8.758313294727783 1.6566535763423243E-4 98-99 0.0014909882187080917 0.0 0.0 9.39086504651469 1.6566535763423243E-4 100-101 0.001615237236933766 0.0 0.0 10.308733960487157 1.6566535763423243E-4 102-103 0.0017394862551594404 0.0 0.0 11.055926139756952 1.6566535763423243E-4 104-105 0.0021950659886535795 0.0 0.0 11.73097105577704 1.6566535763423243E-4 106-107 0.002277898667470696 0.0 0.0 12.467726317515881 1.6566535763423243E-4 108-109 0.002319315006879254 0.0 0.0 13.1221044801711 1.6566535763423243E-4 110-111 0.0024021476856963702 0.0 0.0 13.803030516387203 1.6566535763423243E-4 112-113 0.0024021476856963702 0.0 0.0 14.643243793968622 1.6566535763423243E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 205 0.0 58.07421 2 ATAACGC 260 0.0 48.08675 3 AGTACTC 1240 0.0 47.532753 5 GTACAAA 1255 0.0 43.162327 1 AAGTACT 1495 0.0 39.026924 4 GAACAAA 1725 0.0 38.30373 1 AAAAAGT 1650 0.0 34.633347 1 AAAGTAC 1700 0.0 34.320736 3 TATCACG 125 1.2737164E-6 33.3346 2 GGACCGT 90 2.3880787E-4 33.067284 6 AAAAGTA 1755 0.0 32.900448 2 ATATAGG 270 0.0 30.865366 2 ATGGGGG 1845 0.0 30.655378 5 GTATAAC 410 0.0 30.488962 1 GCGTATC 100 4.427385E-4 29.763033 1 TAACGCA 445 0.0 29.433516 4 CTTACTA 310 0.0 28.800539 9 ATCACGC 150 5.269814E-6 27.783455 3 GTACATA 715 0.0 27.473568 1 TCACGCA 175 5.670754E-7 27.216444 4 >>END_MODULE