##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364150_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1238523 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.097054313888396 31.0 31.0 34.0 26.0 34.0 2 30.13278962118588 31.0 31.0 34.0 26.0 34.0 3 30.326834463308312 31.0 31.0 34.0 26.0 34.0 4 33.5272917822277 37.0 35.0 37.0 27.0 37.0 5 33.6590180400364 37.0 35.0 37.0 28.0 37.0 6 33.45848643908914 37.0 35.0 37.0 27.0 37.0 7 33.41013852790784 37.0 35.0 37.0 27.0 37.0 8 33.18862386891483 37.0 35.0 37.0 25.0 37.0 9 34.50358451155126 39.0 35.0 39.0 23.0 39.0 10-11 34.51521610821922 39.0 35.0 39.0 25.0 39.0 12-13 34.40295093429835 39.0 35.0 39.0 23.5 39.0 14-15 35.54212113945401 40.0 36.0 41.0 22.5 41.0 16-17 35.60467024027814 40.0 36.0 41.0 25.0 41.0 18-19 35.545584135296636 40.0 36.0 41.0 24.5 41.0 20-21 35.55723107281818 40.0 36.0 41.0 25.0 41.0 22-23 35.566888140147576 40.0 36.0 41.0 25.0 41.0 24-25 35.56517965350663 40.0 36.0 41.0 25.0 41.0 26-27 35.38855798398576 40.0 35.0 41.0 24.5 41.0 28-29 35.27317013894776 40.0 35.0 41.0 24.0 41.0 30-31 35.34640051093116 40.0 35.0 41.0 25.0 41.0 32-33 35.05397840815229 39.0 35.0 41.0 24.5 41.0 34-35 35.10953409827674 39.5 35.0 41.0 25.0 41.0 36-37 35.09217753727626 40.0 35.0 41.0 25.0 41.0 38-39 34.94824641932367 40.0 35.0 41.0 24.5 41.0 40-41 34.62170504706009 39.0 34.0 41.0 23.5 41.0 42-43 34.38962740296304 39.0 33.0 41.0 22.0 41.0 44-45 34.14266024934539 38.5 33.0 41.0 20.5 41.0 46-47 33.884233478102544 38.0 33.0 40.0 18.0 41.0 48-49 33.60802786867906 38.0 33.0 40.0 15.0 41.0 50-51 33.50281625775218 38.0 33.0 40.0 14.0 41.0 52-53 33.31727751523387 38.0 32.5 40.0 11.0 41.0 54-55 33.015214493392534 38.0 32.0 40.0 8.0 41.0 56-57 32.64237442502077 38.0 31.0 40.0 6.5 41.0 58-59 32.22081543903505 37.0 31.0 40.0 2.0 41.0 60-61 31.908109901875058 36.5 30.0 40.0 2.0 41.0 62-63 31.386849901051495 36.0 29.5 39.0 2.0 41.0 64-65 31.1319325519187 36.0 30.0 39.0 2.0 41.0 66-67 30.67421073326858 35.0 29.5 39.0 2.0 41.0 68-69 30.287003955517985 35.0 29.0 38.0 2.0 40.0 70-71 29.711446214563637 35.0 28.0 37.5 2.0 40.0 72-73 29.484662779778816 34.0 28.0 37.0 2.0 39.0 74-75 29.157276045741582 34.0 28.0 36.5 2.0 39.0 76-77 28.475788499688743 34.0 26.0 36.0 2.0 39.0 78-79 28.0342698520738 34.0 26.0 35.5 2.0 37.0 80-81 27.89098708703835 34.0 26.0 35.0 2.0 37.0 82-83 27.693568468248067 34.0 26.0 35.0 2.0 36.5 84-85 27.42705948940795 34.0 26.0 35.0 2.0 36.0 86-87 27.10425442240475 34.0 25.5 35.0 2.0 36.0 88-89 26.74539229388554 33.0 25.0 35.0 2.0 36.0 90-91 26.573340987611857 33.0 24.5 35.0 2.0 35.0 92-93 26.259351663231122 33.0 23.5 35.0 2.0 35.0 94-95 26.164990880266252 33.0 24.0 35.0 2.0 35.0 96-97 26.067676982986995 33.0 23.5 35.0 2.0 35.0 98-99 25.87894128732369 33.0 22.0 35.0 2.0 35.0 100-101 25.371310827493716 33.0 18.5 35.0 2.0 35.0 102-103 24.147382406301702 30.5 17.0 34.0 2.0 35.0 104-105 24.897410463915485 32.0 18.0 35.0 2.0 35.0 106-107 24.777206801972994 32.5 13.5 35.0 2.0 35.0 108-109 24.66952168025947 33.0 10.0 35.0 2.0 35.0 110-111 24.7867657685808 33.0 10.0 35.0 2.0 35.0 112-113 24.694683102372746 33.0 7.5 35.0 2.0 35.0 114-115 24.522216785639024 33.0 4.5 35.0 2.0 35.0 116-117 24.190542686732506 32.5 2.0 35.0 2.0 35.0 118-119 23.891184903308215 32.0 2.0 35.0 2.0 35.0 120-121 23.6648471606906 32.0 2.0 35.0 2.0 35.0 122-123 23.193406178165446 32.0 2.0 35.0 2.0 35.0 124-125 22.14264773443852 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8183402259343779 1101 2 0.950853621343061 1101 3 0.8713103222595961 1101 4 0.9472407823407281 1101 5 0.7725391091805918 1101 6 1.0111245118079282 1101 7 0.9763717410598787 1101 8 1.0820976451926043 1101 9 1.2509262300085595 1101 10-11 1.1109508895811615 1101 12-13 0.9598794568557381 1101 14-15 0.9515041077229327 1101 16-17 0.9983462810264854 1101 18-19 1.0478546670046924 1101 20-21 0.9787192040746433 1101 22-23 1.137340124691967 1101 24-25 1.1885586661609224 1101 26-27 1.3620282743539178 1101 28-29 1.395250265571562 1101 30-31 1.3275097654918468 1101 32-33 1.4771324159290131 1101 34-35 1.396137227390156 1101 36-37 1.4708216950846875 1101 38-39 1.3784380015191928 1101 40-41 1.3731414506808406 1101 42-43 1.4634375287763106 1101 44-45 1.5422522004042918 1101 46-47 1.6178504796971254 1101 48-49 1.5503714540473084 1101 50-51 1.4064904871321176 1101 52-53 1.457482078452344 1101 54-55 1.6521592961402405 1101 56-57 1.577194081820494 1101 58-59 1.6278097322744216 1101 60-61 1.6115344086784589 1101 62-63 1.5214398198031986 1101 64-65 1.4573401155386065 1101 66-67 1.481682960440093 1101 68-69 1.3815199117443022 1101 70-71 1.3956465613623052 1101 72-73 1.3200625105751023 1101 74-75 1.4351273445521144 1101 76-77 1.280404106860182 1101 78-79 1.3431617495328148 1101 80-81 1.268268084588442 1101 82-83 1.3301637630280112 1101 84-85 1.2667935272701385 1101 86-87 1.151821306798336 1101 88-89 1.1300003664189155 1101 90-91 1.1459353688517346 1101 92-93 1.1892285172622081 1101 94-95 1.0456318861545704 1101 96-97 1.0124537574524268 1101 98-99 0.9101961641983323 1101 100-101 1.156169705737284 1101 102-103 1.1110659542135188 1101 104-105 1.0577955543192346 1101 106-107 1.1288995123301682 1101 108-109 1.0374808430089928 1101 110-111 0.7614439166649376 1101 112-113 0.911067721282464 1101 114-115 0.7917483771127323 1101 116-117 1.0261833002619483 1101 118-119 0.880099418961759 1101 120-121 0.8287572303038218 1101 122-123 0.7360188239880543 1101 124-125 0.6260623456733541 1102 1 0.41817662104166686 1102 2 0.5105093146401494 1102 3 0.36416107712797796 1102 4 0.52144977006418 1102 5 0.4940920654391121 1102 6 0.5663392483846081 1102 7 0.5352833472864091 1102 8 0.6031453527799329 1102 9 0.9459129801920767 1102 10-11 0.6796097124035967 1102 12-13 0.8403961879156867 1102 14-15 0.8665378184609978 1102 16-17 0.7505389224000822 1102 18-19 0.8185083854958677 1102 20-21 0.7183875580456416 1102 22-23 0.7048990183293498 1102 24-25 0.8184559138046481 1102 26-27 0.8877883861024856 1102 28-29 0.9257908274486155 1102 30-31 0.7991244344349653 1102 32-33 0.963894382298399 1102 34-35 0.7848356759421051 1102 36-37 0.7999562092953951 1102 38-39 0.9063412666651658 1102 40-41 0.9541634304119739 1102 42-43 1.020076222953577 1102 44-45 1.021908518192511 1102 46-47 0.9457013995254258 1102 48-49 0.9915788546206628 1102 50-51 1.030674819238854 1102 52-53 1.1642684706728303 1102 54-55 1.1135000550265701 1102 56-57 1.126025753182624 1102 58-59 1.1656403443582093 1102 60-61 1.2204729092634778 1102 62-63 1.046208551885897 1102 64-65 0.9918249869464617 1102 66-67 1.110762330905903 1102 68-69 1.044366872534507 1102 70-71 0.9289306129193484 1102 72-73 0.9015001303919767 1102 74-75 0.9541578279671583 1102 76-77 0.8488306602229514 1102 78-79 0.9872411317814773 1102 80-81 0.9059988664631149 1102 82-83 0.8771041394429844 1102 84-85 0.8256282503907997 1102 86-87 0.7970518736359722 1102 88-89 0.8783436975017231 1102 90-91 0.7810720086888487 1102 92-93 0.7947158206506018 1102 94-95 0.7692866085446965 1102 96-97 0.5783754238775458 1102 98-99 0.6450192623577315 1102 100-101 0.7662243369281398 1102 102-103 0.5288533173409711 1102 104-105 0.7756229202274376 1102 106-107 0.5647586766196433 1102 108-109 0.7251069931625835 1102 110-111 0.7159903888202876 1102 112-113 0.7401418483793663 1102 114-115 0.6989540031886534 1102 116-117 0.5480077923326796 1102 118-119 0.49263875895711706 1102 120-121 0.4767658989476544 1102 122-123 0.6010791050561934 1102 124-125 0.5710741344162535 1103 1 0.21725245578546293 1103 2 0.2523292914989135 1103 3 0.24887788788811704 1103 4 0.23141819278533404 1103 5 0.21891587918363342 1103 6 0.29537306914036066 1103 7 0.27933611500313305 1103 8 0.3866302400331989 1103 9 0.5542941682315359 1103 10-11 0.45327115101906656 1103 12-13 0.5316705528526242 1103 14-15 0.5456921244346802 1103 16-17 0.5333771551885675 1103 18-19 0.4370870339360948 1103 20-21 0.4844375604499973 1103 22-23 0.5318630698622329 1103 24-25 0.5796749309373581 1103 26-27 0.7158522103193263 1103 28-29 0.7123543361301401 1103 30-31 0.653917038975699 1103 32-33 0.6099821895742963 1103 34-35 0.5928210051822873 1103 36-37 0.6966945298875942 1103 38-39 0.7273865820490997 1103 40-41 0.771463878810529 1103 42-43 0.7898265671334457 1103 44-45 0.6593516075534183 1103 46-47 0.6603123174630952 1103 48-49 0.7333869580901293 1103 50-51 0.7804609498140849 1103 52-53 0.9509012902643974 1103 54-55 0.8545249600542988 1103 56-57 1.0708799449936706 1103 58-59 1.0185771381512723 1103 60-61 0.9974593421352012 1103 62-63 0.8124276016704286 1103 64-65 0.7015275941646486 1103 66-67 0.8664525043108178 1103 68-69 0.9622656507033795 1103 70-71 0.7510847544465875 1103 72-73 0.7310185932155022 1103 74-75 0.7106224431892016 1103 76-77 0.7522951074365309 1103 78-79 0.7495985101133726 1103 80-81 0.7600649679960298 1103 82-83 0.7768158535507652 1103 84-85 0.6559054227906316 1103 86-87 0.6920375335500459 1103 88-89 0.6048962767283967 1103 90-91 0.6071931979987575 1103 92-93 0.5640641021852844 1103 94-95 0.7359330706605682 1103 96-97 0.6412873191732515 1103 98-99 0.6117020867283358 1103 100-101 0.52229846605956 1103 102-103 0.46579686353053873 1103 104-105 0.586750245111272 1103 106-107 0.5581340674767787 1103 108-109 0.6707709501239201 1103 110-111 0.666582396010817 1103 112-113 0.7051058512313553 1103 114-115 0.6816865331288895 1103 116-117 0.6537502680174576 1103 118-119 0.45707766116728976 1103 120-121 0.4604235143317652 1103 122-123 0.4006751426687174 1103 124-125 0.3535775376264638 1104 1 0.27026561016780093 1104 2 0.38116119814816685 1104 3 0.4331523618744235 1104 4 0.41996014998600373 1104 5 0.4546000590906658 1104 6 0.3927961796268775 1104 7 0.24783155484119845 1104 8 0.3784723443513158 1104 9 0.5167570746449712 1104 10-11 0.43582295036475927 1104 12-13 0.4363012808864539 1104 14-15 0.6265399829721048 1104 16-17 0.5680087091868913 1104 18-19 0.5340816005261857 1104 20-21 0.5273185844544415 1104 22-23 0.4088637892892777 1104 24-25 0.6507111692317054 1104 26-27 0.5744575632874813 1104 28-29 0.5648065528871697 1104 30-31 0.5976619739579334 1104 32-33 0.6102569125470012 1104 34-35 0.6208496314357461 1104 36-37 0.5857181201517392 1104 38-39 0.772524643809696 1104 40-41 0.8358493073604052 1104 42-43 0.760772069038552 1104 44-45 0.7543456043477192 1104 46-47 0.7160640229600617 1104 48-49 0.8638902457879283 1104 50-51 0.8443214719433456 1104 52-53 0.8150151680240469 1104 54-55 0.9190396700031016 1104 56-57 0.8994791451248645 1104 58-59 0.7146985166261572 1104 60-61 0.8545360622661065 1104 62-63 0.8278488974291562 1104 64-65 0.9212682652548168 1104 66-67 0.7703611119381826 1104 68-69 0.9185676400807594 1104 70-71 0.9045348919277778 1104 72-73 0.7653625559537325 1104 74-75 0.8324985916090348 1104 76-77 0.5746310427575274 1104 78-79 0.597448069658828 1104 80-81 0.6373718329580171 1104 82-83 0.7389346080920731 1104 84-85 0.8463979729500188 1104 86-87 0.7222196527355251 1104 88-89 0.516802076076484 1104 90-91 0.627156101419569 1104 92-93 0.6090719847594741 1104 94-95 0.6923236417372074 1104 96-97 0.7077906284179498 1104 98-99 0.7066320413481719 1104 100-101 0.5409326439914608 1104 102-103 0.17955946697061975 1104 104-105 0.43595067557044587 1104 106-107 0.3886802694167173 1104 108-109 0.45490213722642636 1104 110-111 0.42106085928591597 1104 112-113 0.46635183662495194 1104 114-115 0.4260346616010864 1104 116-117 0.29202899392533155 1104 118-119 0.13973911029206576 1104 120-121 0.29393061942198173 1104 122-123 0.3876003641528918 1104 124-125 0.2665852214903488 1105 1 0.10940495030711261 1105 2 0.254166787527943 1105 3 0.21882841928930574 1105 4 2.174465611517462E-4 1105 5 0.23988578544847883 1105 6 0.02901165846650855 1105 7 0.16817585097772536 1105 8 -0.04748145171881646 1105 9 0.004336901370173507 1105 10-11 0.12169497811447627 1105 12-13 0.18096139106443587 1105 14-15 0.14813997814326285 1105 16-17 0.0708516874531 1105 18-19 0.13360797201672625 1105 20-21 0.21540684756172368 1105 22-23 0.08938586713819063 1105 24-25 0.257127688685884 1105 26-27 0.2899897213353384 1105 28-29 0.4494305272621304 1105 30-31 0.305894538835382 1105 32-33 -0.04531616990137621 1105 34-35 0.13929076186667544 1105 36-37 0.17577091544524137 1105 38-39 0.2453201787443291 1105 40-41 0.25809697040410384 1105 42-43 0.30595087687897404 1105 44-45 0.29469356235293276 1105 46-47 0.2842864472780917 1105 48-49 0.3879831284833841 1105 50-51 0.35037462135161235 1105 52-53 0.455991076147356 1105 54-55 0.4552760738066155 1105 56-57 0.4270045267036551 1105 58-59 0.4124444553723521 1105 60-61 0.34372550159778825 1105 62-63 0.27810583184146864 1105 64-65 0.3658804894577514 1105 66-67 0.3850621062763686 1105 68-69 0.45843673833553567 1105 70-71 0.2945993569126699 1105 72-73 0.27617814666144014 1105 74-75 0.2767164607233852 1105 76-77 0.4155268726536647 1105 78-79 0.17433523983914512 1105 80-81 0.24622993353466427 1105 82-83 0.27947068957066534 1105 84-85 0.2946881619587778 1105 86-87 0.3323050829246128 1105 88-89 0.19864705428635077 1105 90-91 -0.026465325743448176 1105 92-93 -0.15962970171213442 1105 94-95 0.11297678229260555 1105 96-97 0.09439442629889427 1105 98-99 0.14254531542093574 1105 100-101 -0.013168447728286736 1105 102-103 -0.21567185448109072 1105 104-105 -0.03794140795235279 1105 106-107 0.021832454264625767 1105 108-109 -0.0696857013455272 1105 110-111 -0.16567412202245535 1105 112-113 -0.13027522015815407 1105 114-115 -0.23899590091828316 1105 116-117 -0.13676375637948723 1105 118-119 -0.25891766798782 1105 120-121 -0.2702959416004447 1105 122-123 -0.3372635229810754 1105 124-125 -0.15566042953759052 1106 1 -0.11342325673064835 1106 2 -0.020304071206222574 1106 3 -0.08695482497279272 1106 4 -0.017451214842566287 1106 5 -0.28079151323866824 1106 6 -0.15706043203978481 1106 7 -0.09668821973104968 1106 8 -0.10447770995367023 1106 9 -0.09360119485448593 1106 10-11 -0.1104617776607455 1106 12-13 -0.11948957789355319 1106 14-15 -0.12164387512557795 1106 16-17 -0.07974829350877854 1106 18-19 -0.0942464071123652 1106 20-21 -0.09601730830133448 1106 22-23 -0.0841799873150606 1106 24-25 -0.15131598712308403 1106 26-27 0.06614068141419693 1106 28-29 0.028599137070933978 1106 30-31 0.09742878519887199 1106 32-33 -0.30086355737179105 1106 34-35 -0.030589988534146073 1106 36-37 0.009257167064745886 1106 38-39 -0.12698465312688256 1106 40-41 0.0011193236712045973 1106 42-43 0.023268559485671858 1106 44-45 -0.019857485823933985 1106 46-47 0.023911934796785772 1106 48-49 -0.08634539758965332 1106 50-51 -0.00940877596209333 1106 52-53 -0.02605655703219867 1106 54-55 0.013650596420255567 1106 56-57 0.022696443952334278 1106 58-59 0.1431313557813496 1106 60-61 -0.08023838035622077 1106 62-63 -0.009553862444612093 1106 64-65 0.07935934980456238 1106 66-67 0.014450307810673024 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-3.2916531712740635 2216 98-99 -3.352591496212252 2216 100-101 -3.1266186251764125 2216 102-103 -3.0846395705377603 2216 104-105 -3.3681223547216774 2216 106-107 -3.221410668239237 2216 108-109 -3.0601028678467443 2216 110-111 -3.0838758744490526 2216 112-113 -3.300969169993028 2216 114-115 -3.428390912620408 2216 116-117 -3.442542588349024 2216 118-119 -3.4044127851665955 2216 120-121 -3.449335149476912 2216 122-123 -3.3389635874520884 2216 124-125 -3.5110686589735245 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48732.0 3 8246.0 4 5946.0 5 4359.0 6 4936.0 7 4313.0 8 4812.0 9 5502.0 10 6784.0 11 7925.0 12 8644.0 13 9397.0 14 9867.0 15 10103.0 16 9894.0 17 10108.0 18 10192.0 19 10714.0 20 11048.0 21 11792.0 22 13483.0 23 16810.0 24 19351.0 25 19328.0 26 22556.0 27 23586.0 28 27291.0 29 33659.0 30 39567.0 31 47351.0 32 58514.0 33 71126.0 34 85214.0 35 102251.0 36 138216.0 37 216127.0 38 100116.0 39 663.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.146143197711126 16.939874747848496 9.612185713335183 22.3017963411052 2 12.900192525655891 24.29311929694466 36.51862014355453 26.288068033844926 3 22.38990251504215 33.127606568654414 25.988417323556444 18.494073592746997 4 13.317092679618629 19.650298651182073 32.33674776447645 34.69586090472285 5 11.27206737719254 42.91523823276023 31.641872893854313 14.170821496192918 6 29.82720881139328 29.992633374060713 21.498215945986733 18.681941868559274 7 25.253816577167054 34.64478657992505 21.50570255844514 18.59569428446276 8 29.131719254458808 33.40557443849512 21.68734233601932 15.775363971026746 9 31.95692659750145 17.419004069576367 18.531463140511963 32.09260619241022 10-11 23.176149562288877 31.798825331453322 23.426867246223917 21.598157860033883 12-13 26.741208949943037 29.058546948897813 23.006899428374812 21.193344672784345 14-15 27.74426555634164 27.075896189369697 22.469640226132448 22.710198028156213 16-17 22.773408690953435 30.509126401697976 28.406325886007807 18.31113902134078 18-19 24.85524373608464 29.281956200151622 27.89258000179285 17.970220061970892 20-21 21.873334269049877 29.988876872413428 28.23197440788209 19.90581445065461 22-23 20.514657702926673 32.28029674469367 22.053194143816093 25.151851408563562 24-25 21.027236659553925 33.6222647724599 21.31412622816483 24.036372339821344 26-27 23.611556266000218 28.480572539059807 22.372312673187793 25.535558521752183 28-29 23.085526833578506 28.593422785356527 25.6479078141646 22.67314256690037 30-31 25.90303908672405 30.4229614115268 24.037560323652066 19.63643917809709 32-33 19.36905775759201 31.002696920430907 26.827237638573713 22.80100768340337 34-35 17.2697567273043 32.291750344651874 27.673861553404205 22.764631374639627 36-37 23.923756022841886 29.819844992803613 24.60243464549941 21.65396433885509 38-39 26.59137980730056 27.90805527774884 23.838397433376723 21.66216748157388 40-41 21.633052796624806 31.998858503942945 23.89934404512801 22.468744654304242 42-43 24.97686224002705 30.065345169164086 23.19946066621344 21.758331924595428 44-45 26.760279508382535 27.874192840669597 23.598554848714524 21.76697280223334 46-47 22.533038352269845 29.506244689631117 29.21764451807705 18.743072440021983 48-49 24.666838080468235 28.91087077129722 27.66322703514686 18.759064113087685 50-51 20.64322692902996 30.25582529803167 28.228208379475745 20.872739393462624 52-53 19.618877628391697 32.11465054929727 22.71800577558893 25.548466046722098 54-55 20.559156440131943 33.06317575718257 21.7351690748779 24.64249872780759 56-57 23.251493027134558 28.336684054741827 22.588240731393768 25.823582186729844 58-59 22.862601083467194 28.17780959941229 25.604514203377544 23.355075113742974 60-61 25.162888671285007 30.070369852265884 24.26955039118015 20.49719108526896 62-63 19.13542878435917 30.714526390737728 26.773665257922612 23.37637956698049 64-65 17.043870673143797 31.673741142711382 27.743909239075116 23.538478945069706 66-67 23.169380250290633 29.651082872126167 24.74104614720865 22.438490730374554 68-69 25.60541678780882 27.859694879914166 24.030670152316745 22.50421817996027 70-71 21.044471849391925 31.28208518036236 24.202430190717617 23.4710127795281 72-73 24.165476754847887 29.588473678042103 23.56094342400184 22.685106143108168 74-75 25.774593566247066 27.36322059942971 23.9204924046254 22.941693429697818 76-77 21.81987467822019 28.912169319035254 29.21813386364484 20.049822139099714 78-79 23.8164884504454 28.641524872891445 27.61253248419937 19.929454192463787 80-81 19.890721772393256 29.979087582760577 28.115461522895995 22.01472912195017 82-83 19.101808402445375 31.440801542875956 22.926679329322592 26.530710725356077 84-85 19.972912696159927 32.39690199099793 22.16345646972953 25.466728843112605 86-87 22.33307514811856 27.71599356023894 23.17770577429205 26.77322551735045 88-89 22.057836665894907 27.532204129313637 25.881216646845516 24.528742557945943 90-91 24.10208686872373 29.46189077240297 24.643500862685944 21.79252149618735 92-93 18.793001506142577 29.842183509320318 26.67725643598164 24.687558548555458 94-95 16.978246773391014 30.632539855367785 27.818647279640256 24.57056609160094 96-97 22.661702514075188 28.696520769011236 25.24481585501907 23.39696086189451 98-99 24.595856434199007 27.30558797782317 24.441931718704407 23.656623869273417 100-101 20.518253402464047 29.98196889604171 24.604655177934045 24.895122523560197 102-103 23.19846185327231 28.64931996977193 24.219246688993294 23.93297148796246 104-105 24.403526004159108 26.803361325468956 24.558965830792644 24.23414683957929 106-107 20.91895213128999 28.187045764412733 29.169199767197927 21.724802337099344 108-109 22.546619071108395 28.408379293419163 27.62793265339293 21.417068982079517 110-111 19.05989482406625 29.516192326340523 28.27272122864171 23.151191620951515 112-113 18.380723669115433 30.84378336788527 23.46350642811914 27.311986534880155 114-115 19.021831660772573 32.25237101500035 22.845574726901553 25.880222597325524 116-117 21.002619532374567 28.08009836214387 23.584909483485276 27.332372621996292 118-119 20.70792706196197 28.080082114705096 25.799784980162777 25.41220584317016 120-121 22.361830444957274 29.684664771275493 25.012633687941825 22.940871095825408 122-123 18.15200329504252 30.147976921729768 26.31882779511062 25.38119198811709 124-125 16.802579100063358 31.017261696529296 27.1409096492085 25.03924955419884 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8856.0 1 5747.5 2 4321.5 3 5864.0 4 5051.5 5 3796.0 6 2817.5 7 2290.5 8 2095.5 9 1933.0 10 1661.5 11 1441.0 12 1343.0 13 1269.5 14 1301.5 15 1351.5 16 1341.5 17 1424.0 18 1592.0 19 1714.5 20 1860.5 21 2178.5 22 2603.0 23 3119.5 24 3765.5 25 4466.5 26 5538.5 27 6753.0 28 7603.5 29 8739.5 30 10175.0 31 11596.5 32 13017.5 33 14597.5 34 16867.0 35 18549.5 36 19965.5 37 21861.5 38 22502.5 39 22405.5 40 22175.0 41 22471.5 42 22794.5 43 23333.5 44 24722.5 45 26002.0 46 27854.5 47 33331.0 48 43359.0 49 72603.0 50 168627.0 51 182573.0 52 86759.5 53 40699.0 54 28348.5 55 22725.0 56 19027.5 57 16114.5 58 14370.5 59 12996.0 60 11529.5 61 10261.5 62 8747.0 63 6634.0 64 4905.0 65 3813.5 66 2624.0 67 1999.5 68 1504.0 69 912.5 70 690.0 71 584.5 72 582.0 73 574.5 74 404.5 75 290.5 76 223.0 77 141.0 78 86.0 79 40.5 80 22.5 81 20.5 82 22.0 83 16.0 84 10.0 85 7.0 86 6.0 87 4.0 88 3.0 89 4.0 90 4.5 91 3.0 92 1.0 93 3.0 94 4.0 95 3.0 96 1.5 97 0.0 98 0.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49486363999699645 2 0.4812183544431553 3 0.46991456759381944 4 0.4694301195859907 5 0.46999530892845753 6 0.4793613037464787 7 0.46369748482668466 8 0.4905843492611764 9 0.5012422054334074 10-11 0.5054811255019084 12-13 0.5043911174842938 14-15 0.4821065091241745 16-17 0.4844480078286798 18-19 0.47213495429636754 20-21 0.48089538910460283 22-23 0.4965995786917159 24-25 0.483882818486213 26-27 0.49397548531597724 28-29 0.4889695225684142 30-31 0.5015651707719598 32-33 0.4999099734118785 34-35 0.49413696798525336 36-37 0.4531607406564109 38-39 0.4159389853882407 40-41 0.4080667052610247 42-43 0.41577750271896446 44-45 0.4499310872708864 46-47 0.45824744473861206 48-49 0.4553003860243209 50-51 0.44201843647635125 52-53 0.4361646897150881 54-55 0.43725469773270254 56-57 0.4203797587933369 58-59 0.42469942019647594 60-61 0.43563987103994034 62-63 0.43531690570138787 64-65 0.44968886326697205 66-67 0.438950265760103 68-69 0.44145324713388445 70-71 0.4500925699401626 72-73 0.45122294862509615 74-75 0.4549370500184494 76-77 0.4470647698912334 78-79 0.43790062840980754 80-81 0.44597476187361884 82-83 0.4441580818442613 84-85 0.4418973244743941 86-87 0.44876033791863373 88-89 0.4370124737287882 90-91 0.44048435111822715 92-93 0.4498907166035673 94-95 0.44678217521999997 96-97 0.41557564938236913 98-99 0.3878006302668582 100-101 0.3892943449576633 102-103 0.36902826996349686 104-105 0.37181384600851175 106-107 0.39216066233731633 108-109 0.39841811577177005 110-111 0.4167060280673028 112-113 0.4129111853393114 114-115 0.4105696866348062 116-117 0.3806953928187042 118-119 0.453241481991049 120-121 0.4608715381143508 122-123 0.4162215800594741 124-125 0.40931819594791535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1238523.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.918183032737666 #Duplication Level Percentage of deduplicated Percentage of total 1 86.6069343745142 44.09867736385813 2 8.903069794882553 9.066562747381358 3 2.1494555602636587 3.283391149147219 4 0.8149914557374633 1.659915364534299 5 0.4239683080025323 1.0793847953476516 6 0.22593980401737981 0.6902666577202693 7 0.15903422607102724 0.5668413682088042 8 0.1063739183157431 0.4333093314168394 9 0.08546805899047406 0.39166904440168004 >10 0.46241777045278165 4.294560939956384 >50 0.03415172099081183 1.1945035412683296 >100 0.019964167071372482 1.8898710371519891 >500 0.002801988320004924 0.911585894817395 >1k 0.0019263669700033854 2.910333604132851 >5k 0.0019263669700033854 6.472143932078271 >10k+ 0.0015761184300027696 21.05698322857859 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 65672 5.302444928354177 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 47284 3.8177732670285494 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 31322 2.528980083534985 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 19311 1.559195913196606 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 17592 1.4204015589536891 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 17265 1.3939991425270262 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 13840 1.117460071391488 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 12882 1.0401098728081755 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 10976 0.8862168889879316 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 9876 0.797401420886007 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 7246 0.585051710787769 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 7217 0.5827102120832637 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 6913 0.5581648463532772 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 6622 0.5346691179735863 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 6395 0.5163408350107346 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 6329 0.5110119069246191 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 5575 0.4501329406074816 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 5553 0.44835663124544317 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5538 0.44714551122587143 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 5318 0.4293824176054865 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4724 0.3814220648304472 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 4714 0.3806146514840661 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 4357 0.35178999501825964 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 4340 0.3504173923294117 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 3095 0.24989443070496065 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 2674 0.21590232882231497 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 2329 0.18804656837216588 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1963 0.1584952398946164 No Hit TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 1929 0.15575003451692054 No Hit CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT 1503 0.1213542259610843 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.0185333659528324E-4 0.0 12-13 0.0 0.0 0.0 6.459306771049064E-4 0.0 14-15 0.0 0.0 0.0 0.0018166800293575491 0.0 16-17 0.0 0.0 0.0 0.003310394720162645 0.0 18-19 0.0 0.0 0.0 0.004844480078286798 0.0 20-21 0.0 0.0 0.0 0.00662078944032529 0.0 22-23 0.0 0.0 0.0 0.010900080176145296 0.0 24-25 0.0 0.0 0.0 0.01582530158907021 0.0 26-27 0.0 0.0 0.0 0.019862368320975873 0.0 28-29 8.07413346381133E-5 0.0 0.0 0.029147621804358904 0.0 30-31 1.614826692762266E-4 0.0 0.0 0.042550683354285704 0.0 32-33 1.614826692762266E-4 0.0 0.0 0.06427010237193818 0.0 34-35 2.0185333659528327E-4 0.0 0.0 0.09906961760096503 0.0 36-37 2.422240039143399E-4 0.0 0.0 0.14513254901200867 0.0 38-39 2.422240039143399E-4 0.0 0.0 0.2196971715503063 0.0 40-41 2.422240039143399E-4 0.0 0.0 0.3331387467168555 0.0 42-43 2.8259467123339655E-4 0.0 0.0 0.42312496417103274 0.0 44-45 3.229653385524532E-4 0.0 0.0 0.5087511495547519 0.0 46-47 3.6333600587150984E-4 0.0 0.0 0.6230001380676822 0.0 48-49 4.037066731905665E-4 0.0 0.0 0.7236442116940904 0.0 50-51 4.037066731905665E-4 0.0 0.0 0.8296172134066142 0.0 52-53 4.037066731905665E-4 0.0 0.0 0.9680078609763403 0.0 54-55 4.037066731905665E-4 0.0 0.0 1.1090629725891243 0.0 56-57 4.844480078286798E-4 0.0 0.0 1.2659837564582976 0.0 58-59 4.844480078286798E-4 0.0 0.0 1.4505180767737054 0.0 60-61 5.248186751477365E-4 0.0 0.0 1.6236274982378203 0.0 62-63 5.651893424667931E-4 0.0 0.0 1.7891068635786336 0.0 64-65 6.459306771049064E-4 0.0 0.0 1.9849449707433775 0.0 66-67 7.266720117430197E-4 0.0 0.0 2.201210635571564 0.0 68-69 7.266720117430197E-4 0.0 0.0 2.4743989413196203 0.0 70-71 7.670426790620763E-4 0.0 0.0 2.886825678651103 0.0 72-73 8.07413346381133E-4 0.0 0.0 3.21047731854798 0.0 74-75 8.07413346381133E-4 0.0 0.0 3.5096239633821895 0.0 76-77 8.07413346381133E-4 0.0 0.0 3.865773990470908 0.0 78-79 8.07413346381133E-4 0.0 0.0 4.19863014251653 0.0 80-81 8.07413346381133E-4 0.0 0.0 4.562490967063187 0.0 82-83 8.07413346381133E-4 0.0 0.0 5.03010440661982 0.0 84-85 8.07413346381133E-4 0.0 0.0 5.470952093743919 0.0 86-87 8.477840137001897E-4 0.0 0.0 5.91385868490129 0.0 88-89 8.881546810192464E-4 0.0 0.0 6.427535055868967 0.0 90-91 8.881546810192464E-4 0.0 0.0 6.9059678342671065 0.0 92-93 8.881546810192464E-4 0.0 0.0 7.37741648721905 0.0 94-95 8.881546810192464E-4 0.0 0.0 7.930696482826722 0.0 96-97 8.881546810192464E-4 0.0 0.0 8.476427163645731 0.0 98-99 9.28525348338303E-4 4.037066731905665E-5 0.0 9.086104981498124 0.0 100-101 0.0010900080176145296 8.07413346381133E-5 0.0 9.995010185519366 0.0 102-103 0.0012111200195716995 8.07413346381133E-5 0.0 10.713688805133211 0.0 104-105 0.0012918613542098128 8.07413346381133E-5 0.0 11.378028506535607 0.0 106-107 0.001372602688847926 8.07413346381133E-5 0.0 12.095213411458648 0.0 108-109 0.0014533440234860394 8.07413346381133E-5 0.0 12.742839656590956 0.0 110-111 0.001493714690805096 8.07413346381133E-5 0.0 13.428656553007091 0.0 112-113 0.0015340853581241526 1.2111200195716996E-4 0.0 14.259646369102551 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 160 0.0 63.246372 2 AGTACTC 1170 0.0 50.88519 5 ATAACGC 225 0.0 50.27048 3 AAGTACT 1430 0.0 38.71586 4 GAACAAA 1455 0.0 36.823196 1 TATCACG 85 1.706168E-4 35.01529 2 AAAAAGT 1660 0.0 32.992992 1 AAAGTAC 1675 0.0 32.697533 3 ATCACGC 100 4.42537E-4 29.765417 3 AAAAGTA 1850 0.0 29.602118 2 GTATAAC 425 0.0 28.01451 1 CATGGGG 4630 0.0 27.643908 4 GTACAAA 1090 0.0 27.307722 1 TAACGCA 460 0.0 25.882969 4 ATGGGAG 2480 0.0 25.4467 5 AGAACAA 1280 0.0 24.182436 2 ATGGGGG 1515 0.0 23.578484 5 GTATAGG 280 2.59206E-9 23.387112 1 GTACAAG 725 0.0 22.991219 1 ATGGGGA 2650 0.0 22.690952 5 >>END_MODULE